Chy8G148220 (gene) Cucumber (hystrix) v1

Overview


Name	Chy8G148220
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	RING-type E3 ubiquitin transferase
Location	chrH08: 4970739 .. 4971638 (-)
RNA-Seq Expression	Chy8G148220
Synteny	Chy8G148220

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCAGCTGGTCGGAACACCCATTGGTGTTATAGATGCAGAAGGTCGGTACGTCTCCGTGGTAGAGATGCAGTTTGCCCCAACTGCAATGGAGGATTTATCCAAGAACTTGATGATATGGTGCCTGGAAATCCATTTGATTTCTTTGGTCTAGATCATGATGAAGATCGCGATCATGGTCTGGGTGCATTCTCTGCTTTTATGAGACAACGTTTAGCCGAAAGAAATGACATGAGGGGAAGATCAGAGTCACTTTTTGAGCATAGCCCGGGATTTGGTCCTCTATTAATATTTGGTGGTCAAATACCGTTGAGAAACAGCAGGTTAGAAGCTCTCTTCAATGGGGCGCCTGGCATTGGTATTACACGGGGCGATAGTGGTGACTACTTCATAGGCCCTGGACTGGAGGAGCTATTCGAACAGCTTTCAGAAAATGGTCATCGCGGCCCTCCACCAGCATCTAGATCATCAATCGATGCAATGCCAACAGTTAAGATCACGCAACGACATCTTCGCTCAGATTCACATTGTCCAGTTTGTAAAGAGAAATTTGAACTGGGGTCTGAAGCAAGGCAGATGGCTTGTAATCACATGTACCACTCAGATTGTATTGTTCCATGGCTAGTCCAGCACAATTCTTGCCCTGTCTGCCGCCAGGAACTGCCACCACAGGGAATAGGAGGTGGGGGCGGGGGACATAGCACCAACGATCAAAACCGAAGCAACAGCTACAACAACGGGAGTGGCTCCCGGGTCAATCCAGGTAGACGCAATCCATTCTCTTATCTGTGGCCGTTTCGTTCATCTAGTTCTAACTCCAACCATGGCGGAGCAGGGAGCAGTGAACCGTCGAACCACCAAATGGGGTATTCAGGATATCGATACACTAATTCACCATGA

mRNA sequence

ATGTCAGCTGGTCGGAACACCCATTGGTGTTATAGATGCAGAAGGTCGGTACGTCTCCGTGGTAGAGATGCAGTTTGCCCCAACTGCAATGGAGGATTTATCCAAGAACTTGATGATATGGTGCCTGGAAATCCATTTGATTTCTTTGGTCTAGATCATGATGAAGATCGCGATCATGGTCTGGGTGCATTCTCTGCTTTTATGAGACAACGTTTAGCCGAAAGAAATGACATGAGGGGAAGATCAGAGTCACTTTTTGAGCATAGCCCGGGATTTGGTCCTCTATTAATATTTGGTGGTCAAATACCGTTGAGAAACAGCAGGTTAGAAGCTCTCTTCAATGGGGCGCCTGGCATTGGTATTACACGGGGCGATAGTGGTGACTACTTCATAGGCCCTGGACTGGAGGAGCTATTCGAACAGCTTTCAGAAAATGGTCATCGCGGCCCTCCACCAGCATCTAGATCATCAATCGATGCAATGCCAACAGTTAAGATCACGCAACGACATCTTCGCTCAGATTCACATTGTCCAGTTTGTAAAGAGAAATTTGAACTGGGGTCTGAAGCAAGGCAGATGGCTTGTAATCACATGTACCACTCAGATTGTATTGTTCCATGGCTAGTCCAGCACAATTCTTGCCCTGTCTGCCGCCAGGAACTGCCACCACAGGGAATAGGAGGTGGGGGCGGGGGACATAGCACCAACGATCAAAACCGAAGCAACAGCTACAACAACGGGAGTGGCTCCCGGGTCAATCCAGGTAGACGCAATCCATTCTCTTATCTGTGGCCGTTTCGTTCATCTAGTTCTAACTCCAACCATGGCGGAGCAGGGAGCAGTGAACCGTCGAACCACCAAATGGGGTATTCAGGATATCGATACACTAATTCACCATGA

Coding sequence (CDS)

ATGTCAGCTGGTCGGAACACCCATTGGTGTTATAGATGCAGAAGGTCGGTACGTCTCCGTGGTAGAGATGCAGTTTGCCCCAACTGCAATGGAGGATTTATCCAAGAACTTGATGATATGGTGCCTGGAAATCCATTTGATTTCTTTGGTCTAGATCATGATGAAGATCGCGATCATGGTCTGGGTGCATTCTCTGCTTTTATGAGACAACGTTTAGCCGAAAGAAATGACATGAGGGGAAGATCAGAGTCACTTTTTGAGCATAGCCCGGGATTTGGTCCTCTATTAATATTTGGTGGTCAAATACCGTTGAGAAACAGCAGGTTAGAAGCTCTCTTCAATGGGGCGCCTGGCATTGGTATTACACGGGGCGATAGTGGTGACTACTTCATAGGCCCTGGACTGGAGGAGCTATTCGAACAGCTTTCAGAAAATGGTCATCGCGGCCCTCCACCAGCATCTAGATCATCAATCGATGCAATGCCAACAGTTAAGATCACGCAACGACATCTTCGCTCAGATTCACATTGTCCAGTTTGTAAAGAGAAATTTGAACTGGGGTCTGAAGCAAGGCAGATGGCTTGTAATCACATGTACCACTCAGATTGTATTGTTCCATGGCTAGTCCAGCACAATTCTTGCCCTGTCTGCCGCCAGGAACTGCCACCACAGGGAATAGGAGGTGGGGGCGGGGGACATAGCACCAACGATCAAAACCGAAGCAACAGCTACAACAACGGGAGTGGCTCCCGGGTCAATCCAGGTAGACGCAATCCATTCTCTTATCTGTGGCCGTTTCGTTCATCTAGTTCTAACTCCAACCATGGCGGAGCAGGGAGCAGTGAACCGTCGAACCACCAAATGGGGTATTCAGGATATCGATACACTAATTCACCATGA

Protein sequence

MSAGRNTHWCYRCRRSVRLRGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHGLGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIGITRGDSGDYFIGPGLEELFEQLSENGHRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVCKEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQGIGGGGGGHSTNDQNRSNSYNNGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSGYRYTNSP*

Homology

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BLAST of Chy8G148220 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 1.3e-88
Identity = 185/322 (57.45%), Postives = 226/322 (70.19%), Query Frame = 0


Query: 1   MSAGRNTHWCYRCRRSVRLRGRDAVCPNCNGGFIQELDDMVPGNPFDFF----GLDHDED 60
           MS+ RNTHWC+RC+R+VRL G++ VC  C GGF++EL DM   +PFD F    G+   + 
Sbjct: 1   MSSSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEEL-DMAQASPFDMFRSHRGVVERDQ 60

Query: 61  RDHGLGAFSAFMRQRLAERN---DMRGRS-ESLFEHSPGFGPLLIFGGQIPLR---NSRL 120
               + AFS FMR RLAER+   ++RGR+  S  E+ PG  PLLIFGGQ+P R   ++ +
Sbjct: 61  TFDLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAPLLIFGGQVPYRLTGDNAV 120

Query: 121 EALFN-GAPGIGITRGDSGDYFIGPGLEELFEQLSE-NGHRGPPPASRSSIDAMPTVKIT 180
           EALFN G+PGIGITRG++GDYF GPGLEELFEQLS     RGPPPA RS+IDA+PT+KI 
Sbjct: 121 EALFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRGPPPAPRSAIDALPTIKIA 180

Query: 181 QRHLR-SDSHCPVCKEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQGI 240
           QRHLR SDS+CPVCK++FELGSEA+QM CNH+YHSDCIVPWLVQHNSCPVCRQELP    
Sbjct: 181 QRHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASG 240

Query: 241 GGGGGGHSTNDQN-RSNSYNNGSGSRVNPG-RRNPFSYLWPFRSSSSNS----NHGGAGS 294
                  +T  +N RS+S ++ S SR N   RRNPFS  WPFRSS S+S    N GG  +
Sbjct: 241 PSSSQNRTTPTRNYRSSSSSSSSNSRENGNERRNPFSSFWPFRSSGSSSSSTQNRGGTRN 300

[Close]

BLAST of Chy8G148220 vs. ExPASy Swiss-Prot
Match: Q94AK4 (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 5.4e-79
Identity = 170/306 (55.56%), Postives = 207/306 (67.65%), Query Frame = 0


Query: 1   MSAGRNTHWCYRCRRSVRLRGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60
           MS+ RNTHWC+RC+R+V LR RDAVC  C GGF++E+ D+ P           D +RD  
Sbjct: 1   MSSIRNTHWCHRCQRAVWLRARDAVCSYCGGGFVEEI-DIGPSR------AHRDVERDPT 60

Query: 61  ---LGAFSAFMRQRLAERN---DMRGRSESLFEHS-PGFGPLLIFGGQIPLR-----NSR 120
              + AFSAFMR RLAER+   ++ GR  S    S     PLLIFGGQ P R     NS 
Sbjct: 61  FDLMEAFSAFMRSRLAERSYDREISGRLGSAGSESFSNLAPLLIFGGQAPFRLAGGDNSS 120

Query: 121 LEALFNG-APGIGITRG-DSGDYFIGPGLEELFEQLSE-NGHRGPPPASRSSIDAMPTVK 180
           +EA  NG APGIGI RG ++GDYF GPGLEEL EQLS    HRGPPPA +SSIDA+PT+K
Sbjct: 121 VEAFVNGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSGTHHRGPPPAPKSSIDALPTIK 180

Query: 181 ITQRHLR-SDSHCPVCKEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQ 240
           ITQ+HL+ SDSHCPVCK++FEL SEA+QM C+H+YHSDCIVPWLVQHNSCPVCR+ELP +
Sbjct: 181 ITQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPSR 240

Query: 241 GIGGGGGGHSTNDQNRSNSYNNGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPS 291
                  G S++ Q+  N   NG   R N  RRN FS LWPFRSSSS+S      ++  +
Sbjct: 241 -------GSSSSTQSSQNRSTNG---RENSRRRNIFSNLWPFRSSSSSSTQNRRDTNNTA 289

[Close]

BLAST of Chy8G148220 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 1.1e-34
Identity = 104/312 (33.33%), Postives = 146/312 (46.79%), Query Frame = 0


Query: 9   WCYRCRRSVRL---RGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDE---------- 68
           +CY+C ++V +      D  CP CN GF++E +D  P    +F     D           
Sbjct: 22  FCYQCNQTVTISISSSADPFCPICNQGFLEEYEDPNPNQSLNFNPNSSDSFFPMADPFST 81

Query: 69  ----------DRDHGLGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQI-PL 128
                         G+   S F           + RS      S  F P       +  L
Sbjct: 82  LLPLIFGSSAAAPSGMDFMSLF-----GPSMQPQARSTQQNPQSDAFDPFTFLQNHLQTL 141

Query: 129 RNS--RLEALFNGAPGIGITR--GDSGDYFIGPGLEELFEQLSEN--GHRGPPPASRSSI 188
           R+S    E +    P     R  G+ GDYF GPGLE+L +QL+EN     G PPAS+S+I
Sbjct: 142 RSSGTHFEFVIENHPSDPGNRMPGNFGDYFFGPGLEQLIQQLAENDPNRYGTPPASKSAI 201

Query: 189 DAMPTVKITQRHLRSD-SHCPVCKEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVC 248
           DA+PTVK+T+  L+S+ + C VC ++FE GS+ +QM C H++H DC++PWL  HNSCPVC
Sbjct: 202 DALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVC 261

Query: 249 RQELPPQGIGGGGGGHSTNDQNRS-NSYNNGSGSRVNPGRRNP---FSYLWPFRSSSSNS 286
           R ELP             + +NRS  S  +G G     G++ P       WPFR    + 
Sbjct: 262 RFELPTD---------DPDYENRSQGSQGSGDGQGSVEGQQTPRFSIQLPWPFRRQDGSG 319

[Close]

BLAST of Chy8G148220 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.4e-34
Identity = 101/298 (33.89%), Postives = 148/298 (49.66%), Query Frame = 0


Query: 9   WCYRCRRSVRLR----GRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHGL--- 68
           +CY+C R+V +       D  CP CN GF++E D+  P     F   + +    H L   
Sbjct: 24  FCYQCNRTVNVTISPPSSDPTCPICNEGFLEEYDNPNPNQGSGFLNPNPNSIPFHDLFLT 83

Query: 69  --GAFSAFMRQRL-AERNDMRGRSESLFEHSP--------------GFGPLLIFGGQI-P 128
               F++ +     +  +     S S+  ++P               F P       +  
Sbjct: 84  LSDPFASLLPLLFPSSSSTTTSSSASIDPNNPSLSGPTRSGRGDPFAFDPFTFIQNHLND 143

Query: 129 LRNS--RLEALFNGAPGIGITR--GDSGDYFIGPGLEELFEQLSEN--GHRGPPPASRSS 188
           LR+S  ++E +    P     R   + GDYFIGPGLE+L +QL+EN     G PPAS+S+
Sbjct: 144 LRSSGAQIEFVIQNNPSDQGFRLPANIGDYFIGPGLEQLIQQLAENDPNRYGTPPASKSA 203

Query: 189 IDAMPTVKITQRHLRSD-SHCPVCKEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPV 248
           I+A+P V IT+ +L S+ + C VC + FE G+EA+QM C H+YH DC++PWL  HNSCPV
Sbjct: 204 IEALPLVNITKSNLNSEFNQCAVCMDDFEEGTEAKQMPCKHLYHKDCLLPWLELHNSCPV 263

Query: 249 CRQELP------PQGIGGGGGGHSTNDQNRSNSYNNGSGSRVNPGRRNPFSYLWPFRS 269
           CR ELP       + + G  G    ND + S   ++G    V    R   S  WPF++
Sbjct: 264 CRHELPTDDPDYERRVRGAQGTSGGNDGDNSGQRSDGDNRTVERSFR--ISLPWPFQA 319

[Close]

BLAST of Chy8G148220 vs. ExPASy Swiss-Prot
Match: O22283 (Probable E3 ubiquitin-protein ligase RHC2A OS=Arabidopsis thaliana OX=3702 GN=RHC2A PE=2 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 5.2e-29
Identity = 90/261 (34.48%), Postives = 130/261 (49.81%), Query Frame = 0


Query: 7   THWCYRCRRSVRLRGRDAV-CPNCNGGFIQELDDMVPGNPFDFFGLDHDEDR-------- 66
           ++WCY C R V +   D++ CP+C+GGF++ + + +   P D F                
Sbjct: 5   SYWCYSCSRFVWV--SDSISCPDCDGGFLELIQEPLDFTPSDSFTTTTTTQHRSPTRFPP 64

Query: 67  -DHGLGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQIPLRNSRL--EALFN 126
                   SA M    +    +  R+ S    SP   P+++  G     +S +  E L  
Sbjct: 65  PSSSSSTPSASMHADNSPTPTIVTRTRS--NRSP--NPVIVLRGSAAAPSSDVVSEGLDR 124

Query: 127 GAPGIGITRG-DSG---------DYFIGPGLEELFEQLSE---NGHRG-----PPPASRS 186
            A  +    G DSG         ++ +G G + L +Q+S+   N +R       PPAS+S
Sbjct: 125 SAFQMYYDDGTDSGLRPLPPSMTEFLLGSGFDRLLDQISQIELNTNRNLRSCEHPPASKS 184

Query: 187 SIDAMPTVKITQRHLRSD--SHCPVCKEKFELGSEARQMACNHMYHSDCIVPWLVQHNSC 236
           +I+A+P ++I   HL SD  SHC VCKE F L S AR+M CNH+YH DCI+PWL   NSC
Sbjct: 185 AIEALPLIEIDPTHLLSDSQSHCAVCKENFVLKSSAREMPCNHIYHPDCILPWLAIRNSC 244

[Close]

BLAST of Chy8G148220 vs. ExPASy TrEMBL
Match: A0A0A0L0T9 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G419610 PE=4 SV=1)

HSP 1 Score: 630.6 bits (1625), Expect = 3.4e-177
Identity = 297/299 (99.33%), Postives = 299/299 (100.00%), Query Frame = 0


Query: 1   MSAGRNTHWCYRCRRSVRLRGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60
           MSAGRNTHWCYRCRRSVRLRGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG
Sbjct: 1   MSAGRNTHWCYRCRRSVRLRGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60

Query: 61  LGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120
           LGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG
Sbjct: 61  LGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120

Query: 121 ITRGDSGDYFIGPGLEELFEQLSENGHRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180
           ITRGDSGDYFIGPGLEELFEQLSENGHRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC
Sbjct: 121 ITRGDSGDYFIGPGLEELFEQLSENGHRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180

Query: 181 KEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQGIGGGGGGHSTNDQNR 240
           KEKFELGSEARQMACNHMYHSDCIVPWL+QHNSCPVCRQELPPQGIGGGGGGHSTNDQNR
Sbjct: 181 KEKFELGSEARQMACNHMYHSDCIVPWLIQHNSCPVCRQELPPQGIGGGGGGHSTNDQNR 240

Query: 241 SNSYNNGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSGYRYTNSP 300
           SNSYNNGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSGYRYT+SP
Sbjct: 241 SNSYNNGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSGYRYTSSP 299

[Close]

BLAST of Chy8G148220 vs. ExPASy TrEMBL
Match: A0A1S3C178 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103495857 PE=4 SV=1)

HSP 1 Score: 618.6 bits (1594), Expect = 1.4e-173
Identity = 292/299 (97.66%), Postives = 295/299 (98.66%), Query Frame = 0


Query: 1   MSAGRNTHWCYRCRRSVRLRGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60
           MSAGRNTHWCYRCRRSVRL GRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG
Sbjct: 1   MSAGRNTHWCYRCRRSVRLCGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60

Query: 61  LGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120
           LGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG
Sbjct: 61  LGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120

Query: 121 ITRGDSGDYFIGPGLEELFEQLSENGHRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180
           ITRGDSGDYFIGPGLEELFE+LS NGHRGPPPASRSSIDAMPTVKIT RHLRSDSHCPVC
Sbjct: 121 ITRGDSGDYFIGPGLEELFERLSANGHRGPPPASRSSIDAMPTVKITHRHLRSDSHCPVC 180

Query: 181 KEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQGIGGGGGGHSTNDQNR 240
           KEKFELGSEARQMACNHMYHSDCIVPWL+QHNSCPVCRQELP QGIGGGGGGHSTNDQNR
Sbjct: 181 KEKFELGSEARQMACNHMYHSDCIVPWLIQHNSCPVCRQELPSQGIGGGGGGHSTNDQNR 240

Query: 241 SNSYNNGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSGYRYTNSP 300
           SNSY+NGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSGYRYTNSP
Sbjct: 241 SNSYSNGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSGYRYTNSP 299

[Close]

BLAST of Chy8G148220 vs. ExPASy TrEMBL
Match: A0A5A7SIY2 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold447G00710 PE=4 SV=1)

HSP 1 Score: 618.6 bits (1594), Expect = 1.4e-173
Identity = 292/299 (97.66%), Postives = 295/299 (98.66%), Query Frame = 0


Query: 1   MSAGRNTHWCYRCRRSVRLRGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60
           MSAGRNTHWCYRCRRSVRL GRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG
Sbjct: 1   MSAGRNTHWCYRCRRSVRLCGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60

Query: 61  LGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120
           LGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG
Sbjct: 61  LGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120

Query: 121 ITRGDSGDYFIGPGLEELFEQLSENGHRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180
           ITRGDSGDYFIGPGLEELFE+LS NGHRGPPPASRSSIDAMPTVKIT RHLRSDSHCPVC
Sbjct: 121 ITRGDSGDYFIGPGLEELFERLSANGHRGPPPASRSSIDAMPTVKITHRHLRSDSHCPVC 180

Query: 181 KEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQGIGGGGGGHSTNDQNR 240
           KEKFELGSEARQMACNHMYHSDCIVPWL+QHNSCPVCRQELP QGIGGGGGGHSTNDQNR
Sbjct: 181 KEKFELGSEARQMACNHMYHSDCIVPWLIQHNSCPVCRQELPSQGIGGGGGGHSTNDQNR 240

Query: 241 SNSYNNGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSGYRYTNSP 300
           SNSY+NGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSGYRYTNSP
Sbjct: 241 SNSYSNGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSGYRYTNSP 299

[Close]

BLAST of Chy8G148220 vs. ExPASy TrEMBL
Match: A0A6J1DKF0 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111021749 PE=4 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 5.2e-157
Identity = 275/309 (89.00%), Postives = 286/309 (92.56%), Query Frame = 0


Query: 1   MSAGRNTHWCYRCRRSVRLRGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60
           MS+GRNTHWCYRCRRSV LRGRDAVCPNCNGGFIQELDDMVP NPFDFFGLDHDEDR  G
Sbjct: 1   MSSGRNTHWCYRCRRSVHLRGRDAVCPNCNGGFIQELDDMVPSNPFDFFGLDHDEDRGQG 60

Query: 61  LGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120
           LGAFSAFMRQRLAERNDMRGRSES+FE SPGFGPLLIFGGQIPLRNSRLEALF+GAPGIG
Sbjct: 61  LGAFSAFMRQRLAERNDMRGRSESVFERSPGFGPLLIFGGQIPLRNSRLEALFSGAPGIG 120

Query: 121 ITRGDSGDYFIGPGLEELFEQLSENGHRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180
           ITRGDSGDYFIGPGLEELFEQLS NG+RGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC
Sbjct: 121 ITRGDSGDYFIGPGLEELFEQLSVNGNRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180

Query: 181 KEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQGIGGG----GGGHSTN 240
           K+KFELGSEARQM CNHMYHSDCI+PWLVQHNSCPVCRQELPPQGIGGG    GGGHSTN
Sbjct: 181 KDKFELGSEARQMPCNHMYHSDCIIPWLVQHNSCPVCRQELPPQGIGGGGGGAGGGHSTN 240

Query: 241 DQNR-SNSYNNGS-GSRVNPGRRNPFSYLWPFRSSSSNSNH----GGAGSSEPSNHQMGY 300
            QNR S+SYNN S GSRVNPGRRNPFSYLWPFRSS+S SNH    GGAGSSEP NHQM Y
Sbjct: 241 GQNRSSSSYNNNSNGSRVNPGRRNPFSYLWPFRSSNSRSNHSGGGGGAGSSEPMNHQMEY 300

[Close]

BLAST of Chy8G148220 vs. ExPASy TrEMBL
Match: A0A6J1EAP6 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432344 PE=4 SV=1)

HSP 1 Score: 560.8 bits (1444), Expect = 3.4e-156
Identity = 269/294 (91.50%), Postives = 280/294 (95.24%), Query Frame = 0


Query: 1   MSAGRNTHWCYRCRRSVRLRGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60
           MS+G+NTHWCYRCRRS+RL GRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG
Sbjct: 1   MSSGQNTHWCYRCRRSIRLCGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60

Query: 61  LGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120
           LGAFSAFMRQRLAERNDMRGR+ESLFE +PGFGPLLIFGGQIPLRN RLEALFNGAPGIG
Sbjct: 61  LGAFSAFMRQRLAERNDMRGRAESLFERNPGFGPLLIFGGQIPLRNRRLEALFNGAPGIG 120

Query: 121 ITRGDSGDYFIGPGLEELFEQLSENGHRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180
           ITRGD GDYFIGPGLEELFEQLSENGHRGPPPASRS IDAMPTVKITQRHLRSDSHCPVC
Sbjct: 121 ITRGDGGDYFIGPGLEELFEQLSENGHRGPPPASRSLIDAMPTVKITQRHLRSDSHCPVC 180

Query: 181 KEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQGIGGGGGGHSTNDQNR 240
           KEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQ +  GG GH+TN QNR
Sbjct: 181 KEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQVM--GGAGHNTNSQNR 240

Query: 241 SNSY-NNGSG-SRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSG 293
           +NSY NNG+G SRVNPGRRNPFSYLWPFRSS+S+SNHGGAGSSE  NHQMGYSG
Sbjct: 241 NNSYNNNGNGSSRVNPGRRNPFSYLWPFRSSNSSSNHGGAGSSESRNHQMGYSG 292

[Close]

BLAST of Chy8G148220 vs. NCBI nr
Match: XP_004144313.1 (probable E3 ubiquitin-protein ligase RHC1A [Cucumis sativus] >XP_004144314.1 probable E3 ubiquitin-protein ligase RHC1A [Cucumis sativus] >XP_011653845.1 probable E3 ubiquitin-protein ligase RHC1A [Cucumis sativus] >XP_031739891.1 probable E3 ubiquitin-protein ligase RHC1A [Cucumis sativus] >KGN54669.1 hypothetical protein Csa_012618 [Cucumis sativus])

HSP 1 Score: 626 bits (1614), Expect = 1.27e-226
Identity = 297/299 (99.33%), Postives = 299/299 (100.00%), Query Frame = 0


Query: 1   MSAGRNTHWCYRCRRSVRLRGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60
           MSAGRNTHWCYRCRRSVRLRGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG
Sbjct: 1   MSAGRNTHWCYRCRRSVRLRGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60

Query: 61  LGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120
           LGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG
Sbjct: 61  LGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120

Query: 121 ITRGDSGDYFIGPGLEELFEQLSENGHRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180
           ITRGDSGDYFIGPGLEELFEQLSENGHRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC
Sbjct: 121 ITRGDSGDYFIGPGLEELFEQLSENGHRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180

Query: 181 KEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQGIGGGGGGHSTNDQNR 240
           KEKFELGSEARQMACNHMYHSDCIVPWL+QHNSCPVCRQELPPQGIGGGGGGHSTNDQNR
Sbjct: 181 KEKFELGSEARQMACNHMYHSDCIVPWLIQHNSCPVCRQELPPQGIGGGGGGHSTNDQNR 240

Query: 241 SNSYNNGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSGYRYTNSP 299
           SNSYNNGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSGYRYT+SP
Sbjct: 241 SNSYNNGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSGYRYTSSP 299

[Close]

BLAST of Chy8G148220 vs. NCBI nr
Match: XP_008455755.1 (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >XP_008455756.1 PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >XP_008455757.1 PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >KAA0025786.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa] >TYK09669.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa])

HSP 1 Score: 614 bits (1583), Expect = 6.77e-222
Identity = 292/299 (97.66%), Postives = 295/299 (98.66%), Query Frame = 0


Query: 1   MSAGRNTHWCYRCRRSVRLRGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60
           MSAGRNTHWCYRCRRSVRL GRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG
Sbjct: 1   MSAGRNTHWCYRCRRSVRLCGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60

Query: 61  LGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120
           LGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG
Sbjct: 61  LGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120

Query: 121 ITRGDSGDYFIGPGLEELFEQLSENGHRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180
           ITRGDSGDYFIGPGLEELFE+LS NGHRGPPPASRSSIDAMPTVKIT RHLRSDSHCPVC
Sbjct: 121 ITRGDSGDYFIGPGLEELFERLSANGHRGPPPASRSSIDAMPTVKITHRHLRSDSHCPVC 180

Query: 181 KEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQGIGGGGGGHSTNDQNR 240
           KEKFELGSEARQMACNHMYHSDCIVPWL+QHNSCPVCRQELP QGIGGGGGGHSTNDQNR
Sbjct: 181 KEKFELGSEARQMACNHMYHSDCIVPWLIQHNSCPVCRQELPSQGIGGGGGGHSTNDQNR 240

Query: 241 SNSYNNGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSGYRYTNSP 299
           SNSY+NGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSGYRYTNSP
Sbjct: 241 SNSYSNGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSGYRYTNSP 299

[Close]

BLAST of Chy8G148220 vs. NCBI nr
Match: XP_038882645.1 (probable E3 ubiquitin-protein ligase RHC1A [Benincasa hispida] >XP_038882646.1 probable E3 ubiquitin-protein ligase RHC1A [Benincasa hispida] >XP_038882647.1 probable E3 ubiquitin-protein ligase RHC1A [Benincasa hispida] >XP_038882648.1 probable E3 ubiquitin-protein ligase RHC1A [Benincasa hispida] >XP_038882649.1 probable E3 ubiquitin-protein ligase RHC1A [Benincasa hispida] >XP_038882650.1 probable E3 ubiquitin-protein ligase RHC1A [Benincasa hispida])

HSP 1 Score: 589 bits (1519), Expect = 5.02e-212
Identity = 285/307 (92.83%), Postives = 289/307 (94.14%), Query Frame = 0


Query: 1   MSAGRNTHWCYRCRRSVRLRGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60
           MS GRNTHWCYRCRRSVRL GRDAVCP+CNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG
Sbjct: 1   MSTGRNTHWCYRCRRSVRLHGRDAVCPSCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60

Query: 61  LGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120
           LGAFSAFMRQRL ERNDMRGRSESLFE SPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG
Sbjct: 61  LGAFSAFMRQRLVERNDMRGRSESLFERSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120

Query: 121 ITRGDSGDYFIGPGLEELFEQLSENGHRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180
           ITRGDSGDYFIGPGLEELFEQLS NGHRGPPPASRSSIDAMPTV ITQRHLRSDSHCPVC
Sbjct: 121 ITRGDSGDYFIGPGLEELFEQLSANGHRGPPPASRSSIDAMPTVNITQRHLRSDSHCPVC 180

Query: 181 KEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQGIGGGGGG-------- 240
           KEKFELG EARQM CNHMYHSDCIVPWLVQHNSCPVCRQELPPQGIGGGGGG        
Sbjct: 181 KEKFELGCEARQMPCNHMYHSDCIVPWLVQHNSCPVCRQELPPQGIGGGGGGGGGGGGGG 240

Query: 241 HSTNDQNRSNSYNNGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSG 299
           H+TN QNRSNSYN GSGSRVNPGRRNPFSYLWPFRSSSS+SNHGGAGSSEP+NHQMGYSG
Sbjct: 241 HNTNGQNRSNSYN-GSGSRVNPGRRNPFSYLWPFRSSSSSSNHGGAGSSEPTNHQMGYSG 300

[Close]

BLAST of Chy8G148220 vs. NCBI nr
Match: XP_022154478.1 (probable E3 ubiquitin-protein ligase RHC1A [Momordica charantia])

HSP 1 Score: 559 bits (1440), Expect = 6.17e-200
Identity = 275/309 (89.00%), Postives = 286/309 (92.56%), Query Frame = 0


Query: 1   MSAGRNTHWCYRCRRSVRLRGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60
           MS+GRNTHWCYRCRRSV LRGRDAVCPNCNGGFIQELDDMVP NPFDFFGLDHDEDR  G
Sbjct: 1   MSSGRNTHWCYRCRRSVHLRGRDAVCPNCNGGFIQELDDMVPSNPFDFFGLDHDEDRGQG 60

Query: 61  LGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120
           LGAFSAFMRQRLAERNDMRGRSES+FE SPGFGPLLIFGGQIPLRNSRLEALF+GAPGIG
Sbjct: 61  LGAFSAFMRQRLAERNDMRGRSESVFERSPGFGPLLIFGGQIPLRNSRLEALFSGAPGIG 120

Query: 121 ITRGDSGDYFIGPGLEELFEQLSENGHRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180
           ITRGDSGDYFIGPGLEELFEQLS NG+RGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC
Sbjct: 121 ITRGDSGDYFIGPGLEELFEQLSVNGNRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180

Query: 181 KEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQGIGGGGGG----HSTN 240
           K+KFELGSEARQM CNHMYHSDCI+PWLVQHNSCPVCRQELPPQGIGGGGGG    HSTN
Sbjct: 181 KDKFELGSEARQMPCNHMYHSDCIIPWLVQHNSCPVCRQELPPQGIGGGGGGAGGGHSTN 240

Query: 241 DQNRSNS-YNNGS-GSRVNPGRRNPFSYLWPFRSSSSNSNH----GGAGSSEPSNHQMGY 299
            QNRS+S YNN S GSRVNPGRRNPFSYLWPFRSS+S SNH    GGAGSSEP NHQM Y
Sbjct: 241 GQNRSSSSYNNNSNGSRVNPGRRNPFSYLWPFRSSNSRSNHSGGGGGAGSSEPMNHQMEY 300

[Close]

BLAST of Chy8G148220 vs. NCBI nr
Match: XP_023517925.1 (probable E3 ubiquitin-protein ligase RHC1A [Cucurbita pepo subsp. pepo] >XP_023517926.1 probable E3 ubiquitin-protein ligase RHC1A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 558 bits (1438), Expect = 6.64e-200
Identity = 270/294 (91.84%), Postives = 280/294 (95.24%), Query Frame = 0


Query: 1   MSAGRNTHWCYRCRRSVRLRGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60
           MS+G+NTHWCYRCRRS+RL GRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG
Sbjct: 1   MSSGQNTHWCYRCRRSIRLCGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60

Query: 61  LGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120
           LGAFSAFMRQRLAERNDMRGRSESLFE +PGFGPLLIFGGQIPLRN RLEALFNGAPGIG
Sbjct: 61  LGAFSAFMRQRLAERNDMRGRSESLFERNPGFGPLLIFGGQIPLRNRRLEALFNGAPGIG 120

Query: 121 ITRGDSGDYFIGPGLEELFEQLSENGHRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180
           ITRGD GDYFIGPGLEELFEQLSENGHRGPPPASRS IDAMPTVKITQRHLRSDSHCPVC
Sbjct: 121 ITRGDGGDYFIGPGLEELFEQLSENGHRGPPPASRSLIDAMPTVKITQRHLRSDSHCPVC 180

Query: 181 KEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQGIGGGGGGHSTNDQNR 240
           KEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQ +GGGG  H+TN QNR
Sbjct: 181 KEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQVMGGGG--HNTNSQNR 240

Query: 241 SNSYNN-GSGS-RVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSG 292
           +NSYNN G+GS RVNPGRRNPFSYLWPFRSS+S+SNHGGAGSSE  NH MGYSG
Sbjct: 241 NNSYNNNGNGSSRVNPGRRNPFSYLWPFRSSNSSSNHGGAGSSESRNHPMGYSG 292

[Close]

BLAST of Chy8G148220 vs. TAIR 10
Match: AT2G40830.2 (RING-H2 finger C1A )

HSP 1 Score: 327.8 bits (839), Expect = 9.2e-90
Identity = 185/322 (57.45%), Postives = 226/322 (70.19%), Query Frame = 0


Query: 1   MSAGRNTHWCYRCRRSVRLRGRDAVCPNCNGGFIQELDDMVPGNPFDFF----GLDHDED 60
           MS+ RNTHWC+RC+R+VRL G++ VC  C GGF++EL DM   +PFD F    G+   + 
Sbjct: 1   MSSSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEEL-DMAQASPFDMFRSHRGVVERDQ 60

Query: 61  RDHGLGAFSAFMRQRLAERN---DMRGRS-ESLFEHSPGFGPLLIFGGQIPLR---NSRL 120
               + AFS FMR RLAER+   ++RGR+  S  E+ PG  PLLIFGGQ+P R   ++ +
Sbjct: 61  TFDLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAPLLIFGGQVPYRLTGDNAV 120

Query: 121 EALFN-GAPGIGITRGDSGDYFIGPGLEELFEQLSE-NGHRGPPPASRSSIDAMPTVKIT 180
           EALFN G+PGIGITRG++GDYF GPGLEELFEQLS     RGPPPA RS+IDA+PT+KI 
Sbjct: 121 EALFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRGPPPAPRSAIDALPTIKIA 180

Query: 181 QRHLR-SDSHCPVCKEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQGI 240
           QRHLR SDS+CPVCK++FELGSEA+QM CNH+YHSDCIVPWLVQHNSCPVCRQELP    
Sbjct: 181 QRHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASG 240

Query: 241 GGGGGGHSTNDQN-RSNSYNNGSGSRVNPG-RRNPFSYLWPFRSSSSNS----NHGGAGS 294
                  +T  +N RS+S ++ S SR N   RRNPFS  WPFRSS S+S    N GG  +
Sbjct: 241 PSSSQNRTTPTRNYRSSSSSSSSNSRENGNERRNPFSSFWPFRSSGSSSSSTQNRGGTRN 300

[Close]

BLAST of Chy8G148220 vs. TAIR 10
Match: AT2G40830.1 (RING-H2 finger C1A )

HSP 1 Score: 327.8 bits (839), Expect = 9.2e-90
Identity = 185/322 (57.45%), Postives = 226/322 (70.19%), Query Frame = 0


Query: 1   MSAGRNTHWCYRCRRSVRLRGRDAVCPNCNGGFIQELDDMVPGNPFDFF----GLDHDED 60
           MS+ RNTHWC+RC+R+VRL G++ VC  C GGF++EL DM   +PFD F    G+   + 
Sbjct: 1   MSSSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEEL-DMAQASPFDMFRSHRGVVERDQ 60

Query: 61  RDHGLGAFSAFMRQRLAERN---DMRGRS-ESLFEHSPGFGPLLIFGGQIPLR---NSRL 120
               + AFS FMR RLAER+   ++RGR+  S  E+ PG  PLLIFGGQ+P R   ++ +
Sbjct: 61  TFDLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAPLLIFGGQVPYRLTGDNAV 120

Query: 121 EALFN-GAPGIGITRGDSGDYFIGPGLEELFEQLSE-NGHRGPPPASRSSIDAMPTVKIT 180
           EALFN G+PGIGITRG++GDYF GPGLEELFEQLS     RGPPPA RS+IDA+PT+KI 
Sbjct: 121 EALFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRGPPPAPRSAIDALPTIKIA 180

Query: 181 QRHLR-SDSHCPVCKEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQGI 240
           QRHLR SDS+CPVCK++FELGSEA+QM CNH+YHSDCIVPWLVQHNSCPVCRQELP    
Sbjct: 181 QRHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASG 240

Query: 241 GGGGGGHSTNDQN-RSNSYNNGSGSRVNPG-RRNPFSYLWPFRSSSSNS----NHGGAGS 294
                  +T  +N RS+S ++ S SR N   RRNPFS  WPFRSS S+S    N GG  +
Sbjct: 241 PSSSQNRTTPTRNYRSSSSSSSSNSRENGNERRNPFSSFWPFRSSGSSSSSTQNRGGTRN 300

[Close]

BLAST of Chy8G148220 vs. TAIR 10
Match: AT2G40830.3 (RING-H2 finger C1A )

HSP 1 Score: 327.8 bits (839), Expect = 9.2e-90
Identity = 185/322 (57.45%), Postives = 226/322 (70.19%), Query Frame = 0


Query: 1   MSAGRNTHWCYRCRRSVRLRGRDAVCPNCNGGFIQELDDMVPGNPFDFF----GLDHDED 60
           MS+ RNTHWC+RC+R+VRL G++ VC  C GGF++EL DM   +PFD F    G+   + 
Sbjct: 1   MSSSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEEL-DMAQASPFDMFRSHRGVVERDQ 60

Query: 61  RDHGLGAFSAFMRQRLAERN---DMRGRS-ESLFEHSPGFGPLLIFGGQIPLR---NSRL 120
               + AFS FMR RLAER+   ++RGR+  S  E+ PG  PLLIFGGQ+P R   ++ +
Sbjct: 61  TFDLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAPLLIFGGQVPYRLTGDNAV 120

Query: 121 EALFN-GAPGIGITRGDSGDYFIGPGLEELFEQLSE-NGHRGPPPASRSSIDAMPTVKIT 180
           EALFN G+PGIGITRG++GDYF GPGLEELFEQLS     RGPPPA RS+IDA+PT+KI 
Sbjct: 121 EALFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRGPPPAPRSAIDALPTIKIA 180

Query: 181 QRHLR-SDSHCPVCKEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQGI 240
           QRHLR SDS+CPVCK++FELGSEA+QM CNH+YHSDCIVPWLVQHNSCPVCRQELP    
Sbjct: 181 QRHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASG 240

Query: 241 GGGGGGHSTNDQN-RSNSYNNGSGSRVNPG-RRNPFSYLWPFRSSSSNS----NHGGAGS 294
                  +T  +N RS+S ++ S SR N   RRNPFS  WPFRSS S+S    N GG  +
Sbjct: 241 PSSSQNRTTPTRNYRSSSSSSSSNSRENGNERRNPFSSFWPFRSSGSSSSSTQNRGGTRN 300

[Close]

BLAST of Chy8G148220 vs. TAIR 10
Match: AT3G56580.2 (RING/U-box superfamily protein )

HSP 1 Score: 295.8 bits (756), Expect = 3.9e-80
Identity = 170/306 (55.56%), Postives = 207/306 (67.65%), Query Frame = 0


Query: 1   MSAGRNTHWCYRCRRSVRLRGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60
           MS+ RNTHWC+RC+R+V LR RDAVC  C GGF++E+ D+ P           D +RD  
Sbjct: 1   MSSIRNTHWCHRCQRAVWLRARDAVCSYCGGGFVEEI-DIGPSR------AHRDVERDPT 60

Query: 61  ---LGAFSAFMRQRLAERN---DMRGRSESLFEHS-PGFGPLLIFGGQIPLR-----NSR 120
              + AFSAFMR RLAER+   ++ GR  S    S     PLLIFGGQ P R     NS 
Sbjct: 61  FDLMEAFSAFMRSRLAERSYDREISGRLGSAGSESFSNLAPLLIFGGQAPFRLAGGDNSS 120

Query: 121 LEALFNG-APGIGITRG-DSGDYFIGPGLEELFEQLSE-NGHRGPPPASRSSIDAMPTVK 180
           +EA  NG APGIGI RG ++GDYF GPGLEEL EQLS    HRGPPPA +SSIDA+PT+K
Sbjct: 121 VEAFVNGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSGTHHRGPPPAPKSSIDALPTIK 180

Query: 181 ITQRHLR-SDSHCPVCKEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQ 240
           ITQ+HL+ SDSHCPVCK++FEL SEA+QM C+H+YHSDCIVPWLVQHNSCPVCR+ELP +
Sbjct: 181 ITQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPSR 240

Query: 241 GIGGGGGGHSTNDQNRSNSYNNGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPS 291
                  G S++ Q+  N   NG   R N  RRN FS LWPFRSSSS+S      ++  +
Sbjct: 241 -------GSSSSTQSSQNRSTNG---RENSRRRNIFSNLWPFRSSSSSSTQNRRDTNNTA 289

[Close]

BLAST of Chy8G148220 vs. TAIR 10
Match: AT3G56580.1 (RING/U-box superfamily protein )

HSP 1 Score: 295.8 bits (756), Expect = 3.9e-80
Identity = 170/306 (55.56%), Postives = 207/306 (67.65%), Query Frame = 0


Query: 1   MSAGRNTHWCYRCRRSVRLRGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60
           MS+ RNTHWC+RC+R+V LR RDAVC  C GGF++E+ D+ P           D +RD  
Sbjct: 1   MSSIRNTHWCHRCQRAVWLRARDAVCSYCGGGFVEEI-DIGPSR------AHRDVERDPT 60

Query: 61  ---LGAFSAFMRQRLAERN---DMRGRSESLFEHS-PGFGPLLIFGGQIPLR-----NSR 120
              + AFSAFMR RLAER+   ++ GR  S    S     PLLIFGGQ P R     NS 
Sbjct: 61  FDLMEAFSAFMRSRLAERSYDREISGRLGSAGSESFSNLAPLLIFGGQAPFRLAGGDNSS 120

Query: 121 LEALFNG-APGIGITRG-DSGDYFIGPGLEELFEQLSE-NGHRGPPPASRSSIDAMPTVK 180
           +EA  NG APGIGI RG ++GDYF GPGLEEL EQLS    HRGPPPA +SSIDA+PT+K
Sbjct: 121 VEAFVNGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSGTHHRGPPPAPKSSIDALPTIK 180

Query: 181 ITQRHLR-SDSHCPVCKEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQ 240
           ITQ+HL+ SDSHCPVCK++FEL SEA+QM C+H+YHSDCIVPWLVQHNSCPVCR+ELP +
Sbjct: 181 ITQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPSR 240

Query: 241 GIGGGGGGHSTNDQNRSNSYNNGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPS 291
                  G S++ Q+  N   NG   R N  RRN FS LWPFRSSSS+S      ++  +
Sbjct: 241 -------GSSSSTQSSQNRSTNG---RENSRRRNIFSNLWPFRSSSSSSTQNRRDTNNTA 289

The following BLAST results are available for this feature:

BLAST of Chy8G148220 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22197	1.3e-88	57.45	Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q94AK4	5.4e-79	55.56	E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV...	[more]
Q8LPN7	1.1e-34	33.33	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
P0CH30	1.4e-34	33.89	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
O22283	5.2e-29	34.48	Probable E3 ubiquitin-protein ligase RHC2A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]

BLAST of Chy8G148220 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L0T9	3.4e-177	99.33	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G419610 PE...	[more]
A0A1S3C178	1.4e-173	97.66	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103495857 PE=4 ...	[more]
A0A5A7SIY2	1.4e-173	97.66	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A6J1DKF0	5.2e-157	89.00	RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11102174...	[more]
A0A6J1EAP6	3.4e-156	91.50	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432344...	[more]

BLAST of Chy8G148220 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004144313.1	1.27e-226	99.33	probable E3 ubiquitin-protein ligase RHC1A [Cucumis sativus] >XP_004144314.1 pro...	[more]
XP_008455755.1	6.77e-222	97.66	PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >XP_008455756.1...	[more]
XP_038882645.1	5.02e-212	92.83	probable E3 ubiquitin-protein ligase RHC1A [Benincasa hispida] >XP_038882646.1 p...	[more]
XP_022154478.1	6.17e-200	89.00	probable E3 ubiquitin-protein ligase RHC1A [Momordica charantia]	[more]
XP_023517925.1	6.64e-200	91.84	probable E3 ubiquitin-protein ligase RHC1A [Cucurbita pepo subsp. pepo] >XP_0235...	[more]

BLAST of Chy8G148220 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G40830.2	9.2e-90	57.45	RING-H2 finger C1A	[more]
AT2G40830.1	9.2e-90	57.45	RING-H2 finger C1A	[more]
AT2G40830.3	9.2e-90	57.45	RING-H2 finger C1A	[more]
AT3G56580.2	3.9e-80	55.56	RING/U-box superfamily protein	[more]
AT3G56580.1	3.9e-80	55.56	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 177..217 e-value: 1.0E-6 score: 38.3
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 176..218 e-value: 2.1E-10 score: 40.8
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 177..218 score: 11.972923
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 139..224 e-value: 8.4E-22 score: 78.6
IPR039525	E3 ubiquitin-protein ligase RNF126-like, zinc-ribbon	PFAM	PF14369	zinc_ribbon_9	coord: 6..37 e-value: 4.0E-12 score: 46.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 225..299
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 263..299
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 231..255
None	No IPR available	PANTHER	PTHR15710:SF86	E3 UBIQUITIN-PROTEIN LIGASE RHC1A-RELATED	coord: 2..284
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 2..284
None	No IPR available	CDD	cd16667	RING-H2_RNF126_like	coord: 177..218 e-value: 1.14207E-23 score: 88.914
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 157..224

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy8G148220.1	Chy8G148220.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0061630	ubiquitin protein ligase activity