Chy8G148070 (gene) Cucumber (hystrix) v1

Overview


Name	Chy8G148070
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	NADH--cytochrome b5 reductase 1
Location	chrH08: 4773222 .. 4778678 (+)
RNA-Seq Expression	Chy8G148070
Synteny	Chy8G148070

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACTTCTTACAGTCTTTGGATCCTCAAATCCTTCTGGGTGTCGCTGTGGCTGTTTTAGCCCTTGCCGTTGGTGCTCTCTATGTATTATCCTCCTCCAAAAAAAGCAAATCTAGAGGTTACCCACCCCTTGAATTCAAGTTTTTTTAATAATTTATTTATTACCCCATTATTTGGATTTCCTTGTTGGGTGTTTCGGATTCATGTTTAGAGGTTGAAATTTCTTGTACTTGATTTCTGTTATCAGTTTCTGGTATCTGTTGTGTGTTAATGCAGCGTGAAGTTCTTTCATGTTATTTTGTGAATATGTGGATTTTGTGTTTGATCCATTCAAACTGCTTGTATGTATATTTTTGTTGTTGTTTCTATATTATCTTCTCTATTTTTAGAATATTATGTTACAGATAAGAAAAAGAAATTTCAGCATACAAGAAGAGAGTTGTTATGCTGACCGCTTGAGATTAATCTGTGTGCTCAAGTTTCCTTATGGTAGTTTACAGTTACTTGAAGTAAGCAAGTAATATTTTGACCCTCTCTAGATAGTTCCTGGAGTCTGGGTATTACTACAGTTTCAGTTTCTTCTTCTGGAACCTGTTTTGTTTTTAAATAAGAGAAGCTAATTAATTAACCTACCCTTATTTGGGGAATATTACAAATAAAGGGAAGAGAGATGATCTTTCTTACAACTCATGAATAGAGTTATATTATATGCTGACTACTTGAGGTTGAGTTTTTATTTTTAGTAGCTTACACTTTGTTGAAGTTGTTGAAGCTAATTACATTTTGACACTCTAGATAGTTTCTGGAGTCTGAACGTTACTACTCTGTCTACATTTCAGTATGAACTAAACCAACAATCTGTTTCAAATTGATCTATTGGTTAAGGAGATTTGATGGGAAATATCACATGAATAGGGATTCACATTTGAGTTGCCTGTGAAAATATTTATATGATTTGATAATAAGAAGATTAATTCAGAAAAGTGTGGAGCAAGGTTGTGTTTGGTTTATCTTTTCAAGTATTTAATTTTGAAAATAAGTCATATTGGAAAAAATTGAAGTGCTCACTTAAAATAGCTTTTGAAACACCTTTAAAGTTTACGTTGCATAGTTTTTATCAAAAGAGTTAAAATGAAAAAATATATTTTTTGAAAACACTTTTTTCCATAGTCAAACGAAATGGGACCCAAATTTAGTATTCATTCAACCTTTTGCAGGATTCTTGTGCTATGGATGGTTTTGTTGTGATGATACGTTAAAACACAGCCTGTTATTGCTACTTTTTTTTTTCCTTCATTTTCTTCATTTTTTTTATCCTTATCATATGATTCAAACATTCAGGTTGCTTGGATCCAGAAATTTTCAAGGAGTTTAAACTTATCAAGCGACTGCAACTGAGCCATAATGTGGCGAAGTTTACATTTACACTCCCTAGTCCTATTTCAGTTCTAGGTCTTCCCATTGGACAACATATAAGTTGCAGGTCTGGTGGTTGATCCTTATTTATTGTTCATAGGATTAGTTGGATTTCCACATGTCATGCCTATGGGTATGTAGCTGAACAAAAACCTAGATTTTCTGATTATGTTGGTTCTTACACTATATGGTGTGAGAATCTCAATACTATTCTCTTCTGTGTTAACTCTGTTAAAATTAAAGGAAACCTAGAAATACAGCAATTTTCCTTTAAAGATACCTGTTAAACTATCAATCCTGCCACTTCCTCATAATAGATTCTGTTTTAGCTCATTGGCATTCACAACTTTTATTGGAATAAGGTCATTTAACTTAACGGTTGTTACTTCTTTTTGTCTTTTCGCTTGAGAGAGGTTGTAGGACTTTAGAGGAAACTTTTACAAATTTTGTCAAGGTGTAGTATCTTTTGTTTCTTTGAGCTGATTGATTATGTTGTTCGTTCAAAGACTATTCTAATCATTTGCATCTTCTGAAGTAAACTAACTGGAAATGGTTTTCGGAATCTCCCTTGGGTTTGAGTGGGGATGAATGCCTTATTGCCTCTTTGCCTGGTGTTTTTGAAGATGATTTTCATATGAGAAAAGGATTTATTTCCCTGGGAAACTATAATTCATTTATCCTACAGGGGAAAGGATGGCCAAGGTGAAGAGGTTATCAAACCGTACACACCTATTACTTTGGATTCTGATCTTGGATATTTTGAATTGGTCATAAAGGTTCGTATTTTGGTATTCTCAGTTGGTGGGGAGTATTTTAATGGCATGTATGCATGTTAATATTAACTCTGGAATGTGTTTTACCAGATGTATCCCCAAGGAAGGATGTCACACCATTTCCGACAAATGCGTGTAGGAGATTTTCTTGCTGTAAAGGGTCCCAAGGTACGTATAAATCAGTAACTTTTTTTTGGTAAAAGAAACATTTCATTAATGAATGAAATTAGGGGAGAACTCCAAATGCCTAAAGGTGATTGCAAAAAGAGTGTCAATTAAAAAGAGAGAAAGGGTGCTTTGTTTTACACCATGAAAAAGCCTCGGACAAAATGAACCATAAAACAATCAAAAGAAAAAGAGTTGCTGAAAAGCCTTTTAATTGTTTTGCCCCATAAACTCCAAAGGAGAGTATGCATTAAAACCAATCAAATCGTCTTCTTTGTACCACCAAAAGGATGATACACCAACAATGATCCAAAGATGTCGTAGATGGAATTTTGTATTAGTAATGGCAGCAATTTAAGTAATGAATTTTTATGCTTTTAAGTAGTGTTTTGTATCAGTTTAAACAGGCACAGCCTTTTAGATATTATACACCCTTTTATAGATTATATAGTCTATCTTCTTTGATCTAATTGGAGATTTTTTTTTGTAATTCACCTCTTGGTGTTACCCAAGCTCCTTGTATTTCGTTGATCAATGAAATTTATTTTTCTTGAAAAAATAACGTTTTCTTTGATTAACAAATGGGGTAATTACTACTTTGTCCAAATTTGCCTTTTACATGTTACATTGCATATATATGAACTCATATTTCAGGTTCTGCGAGTTCAAATCCATTCATGTTTAACAAGTTAATTGGAACATTAATACATTTGAGCTAGTTGGAAGGATTCTCCCTTTTTATAATTTATCTTTTTTAATTGTTTTTTTCAAATCATCTTATGAATAAATTGTTAACTCAATATGGATAAACATTTCATCTTAGTTTCCCATGCCAGCTGCCTTAGTTTTTGTTATAAGTTCTGTCTACCATTCAGCTTCCTTTTGGGTTAGCCACAGACTTCTCCCCCACATTCTATTTCGAGAATTTATTGACAATGTTTTGTTTGTCACACACACAAAAAACAGCTCCCATTTTCTCTCTGAGACAGGATAAACAAAAATCTTCACAATACCTTTAAAGACACCGAGAATATTCAAAATATACAATGAAATCATTCGTGCAGGGACGTTTCAGATATCAACCTGGCCAGGTCAGAGCATTTGGTATGCTTGCTGGCGGCTCTGGCATTACTCCTATGTATCAAGTACAGATTTCCTTTTCTTTTGTCTCCATCATTTTAGTTCATCAATTTACCATCATTTGAACTTCTTGATTGTTGTTATAGATATTTGGCTTTTGTTTCTGCTCCTTAGGTTGCTCGGGCAATTTTAGAAAATCCAAACGACAAGACAAAGGTACATCTCATTTATGCCAACGTCACCTATGAGGACATTCTCTTAAAGGTATTTCGTTGTCTTTCTTCAATAAAATTCCCTCTTGCCCTCATGTTGATCGATTCATAAATTTGGATACTAGGCTATCTTTTTATTTTATAACATGTTGATAGCTAGTTTTTCATAACATGTCTGAGTGATATTGTAATCAATCTACCTCTTTCTCAGTGCTGTGAATTTTAACGGTTGAATGTTTGAACTCATTAGTCGAATACTTGACTAGAATTACATTTGTCAACTCGAGCATAGTGTAACTAGTTAAAGACATATTTTCGACCATAAAGGTTTTGTCATTTTTACCTGGATAAGAAAGGCATGCAAATTTGTGCCATTTTGGGTATTAAAGGGTGATTTGGTTGAAAGGAAACAGAAGACAACAACTTCTTGGGCAAAAGTGTAGGATCTTAACACTCTTGTAGCCTATTCTTAGACTTCCACTTTTGCATTCATTATAATAATTCAATTTCTATGATTTCTCTCTCTAATTTTGTAAGTGTTCCTCACCAAAAGAAACTCGTGTGTTTATAGGAGGAGTTGGACCTTCTTGCCAAAAGCTACCCAGATAGCTTCAAGCTCTACTATGTCTTAAACCAGGTCAGTCTTCTTCCTCAGCCACAGCCACAAATTTAAACTGTTCTCTATGGCTAATCTTTATAGCATTACAAGATCTCCATGTCATCGTACTATGGTTATGCTGTTTTGCTTTACTGACAGATATTGCGTGATATATGTGAACTTTGACAAATCTAGTGAATTAAATTGGATTTCTAGTTCTTCAAGTCTGGGGTTGTTTGATAACCGTTCTTAATAGTTGTTTTCGATTTCTTAAAACAAAAAGGTAAATGAAAATCCTTTTAGTTAACTCTTTTCTAAAAGCTGTTTATTTTTGTGGGCGATGTATCTCTCAAGAGCCACCCCAATCACAATTCTTAAAAACAGTTTCGCGAAACAAGGTACCGAGCACCTGCAGAGTTGTAAAGCTGTGTGTTATGGATGGAATTAGCTTCTCTTAGGCCATGATCGATCTTCTCTTGAGCTTCATTTAAGCTTGCTTACACATTTACTTTTGTTGAATGATTTGCGATGTCATACCTTTTTGATCTTGTGTACCAATTGCTTAATCAGCCTCCTGAAGCGTGGGATGGCGGCGTTGGATTTGTATCGAAGGAGATGATTCAAACCCACTGCCCTGCTCCTGCTTCTGATATTCGGGTATATTTGATTATTAACTCAAACCATTTTTCTTGTTTTCTTGTAACTAATAAACCAAAAGGCTCTCTCTTGTTTTCTTGTAATTTGGAAAAAGCAATTGGAGTTTTTTTTTCTTTGTAAACAAGCCCTTTTCATTCATATTATCAAAAAGACTAATTAAGGAAAAAAACATATAATTACAACTACTATTGTACATAATTAAACTGGAAAAATAAAAGCGGTCGTGCACAATACCATGTTGGATCAGAAAATATTTTTTAAAATTGTGATTGTTTAATCTAAGAGACTCATAAGTAAAAGCAATGCTTATTAATGAGCTAAATGGTTATCAAATCGGACATAACTCAATTCACGAGAGACTATAGTTGTTTTCTATCTAATCTTTTACTTAGGAAGAACCCGCACTTCAACTTCATCAAACTTGGAAAATATTGTCTCATATGAAAACTTATTTTGTAAGCAGATTTTAAGATGTGGGCCACCACCAATGAACAAGGCCATGGCTGCTCATCTTGAAGAACTTGGATATGCACCTGAGATGCTGTTCATGTTCTGA

mRNA sequence

ATGGACTTCTTACAGTCTTTGGATCCTCAAATCCTTCTGGGTGTCGCTGTGGCTGTTTTAGCCCTTGCCGTTGGTGCTCTCTATGTATTATCCTCCTCCAAAAAAAGCAAATCTAGAGGTTGCTTGGATCCAGAAATTTTCAAGGAGTTTAAACTTATCAAGCGACTGCAACTGAGCCATAATGTGGCGAAGTTTACATTTACACTCCCTAGTCCTATTTCAGTTCTAGGTCTTCCCATTGGACAACATATAAGTTGCAGGGGAAAGGATGGCCAAGGTGAAGAGGTTATCAAACCGTACACACCTATTACTTTGGATTCTGATCTTGGATATTTTGAATTGGTCATAAAGATGTATCCCCAAGGAAGGATGTCACACCATTTCCGACAAATGCGTGTAGGAGATTTTCTTGCTGTAAAGGGTCCCAAGGGACGTTTCAGATATCAACCTGGCCAGGTCAGAGCATTTGGTATGCTTGCTGGCGGCTCTGGCATTACTCCTATGTATCAAGTTGCTCGGGCAATTTTAGAAAATCCAAACGACAAGACAAAGGTACATCTCATTTATGCCAACGTCACCTATGAGGACATTCTCTTAAAGGAGGAGTTGGACCTTCTTGCCAAAAGCTACCCAGATAGCTTCAAGCTCTACTATGTCTTAAACCAGCCTCCTGAAGCGTGGGATGGCGGCGTTGGATTTGTATCGAAGGAGATGATTCAAACCCACTGCCCTGCTCCTGCTTCTGATATTCGGATTTTAAGATGTGGGCCACCACCAATGAACAAGGCCATGGCTGCTCATCTTGAAGAACTTGGATATGCACCTGAGATGCTGTTCATGTTCTGA

Coding sequence (CDS)

ATGGACTTCTTACAGTCTTTGGATCCTCAAATCCTTCTGGGTGTCGCTGTGGCTGTTTTAGCCCTTGCCGTTGGTGCTCTCTATGTATTATCCTCCTCCAAAAAAAGCAAATCTAGAGGTTGCTTGGATCCAGAAATTTTCAAGGAGTTTAAACTTATCAAGCGACTGCAACTGAGCCATAATGTGGCGAAGTTTACATTTACACTCCCTAGTCCTATTTCAGTTCTAGGTCTTCCCATTGGACAACATATAAGTTGCAGGGGAAAGGATGGCCAAGGTGAAGAGGTTATCAAACCGTACACACCTATTACTTTGGATTCTGATCTTGGATATTTTGAATTGGTCATAAAGATGTATCCCCAAGGAAGGATGTCACACCATTTCCGACAAATGCGTGTAGGAGATTTTCTTGCTGTAAAGGGTCCCAAGGGACGTTTCAGATATCAACCTGGCCAGGTCAGAGCATTTGGTATGCTTGCTGGCGGCTCTGGCATTACTCCTATGTATCAAGTTGCTCGGGCAATTTTAGAAAATCCAAACGACAAGACAAAGGTACATCTCATTTATGCCAACGTCACCTATGAGGACATTCTCTTAAAGGAGGAGTTGGACCTTCTTGCCAAAAGCTACCCAGATAGCTTCAAGCTCTACTATGTCTTAAACCAGCCTCCTGAAGCGTGGGATGGCGGCGTTGGATTTGTATCGAAGGAGATGATTCAAACCCACTGCCCTGCTCCTGCTTCTGATATTCGGATTTTAAGATGTGGGCCACCACCAATGAACAAGGCCATGGCTGCTCATCTTGAAGAACTTGGATATGCACCTGAGATGCTGTTCATGTTCTGA

Protein sequence

MDFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSHNVAKFTFTLPSPISVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDLGYFELVIKMYPQGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPNDKTKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQTHCPAPASDIRILRCGPPPMNKAMAAHLEELGYAPEMLFMF*

Homology

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BLAST of Chy8G148070 vs. ExPASy Swiss-Prot
Match: Q9ZNT1 (NADH--cytochrome b5 reductase 1 OS=Arabidopsis thaliana OX=3702 GN=CBR1 PE=1 SV=1)

HSP 1 Score: 469.9 bits (1208), Expect = 2.0e-131
Identity = 228/280 (81.43%), Postives = 250/280 (89.29%), Query Frame = 0


Query: 2   DFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSHN 61
           +FL++LD QILLGV VA +A+  GA Y L+SSK  K R CLDPE FKEFKL+KR QLSHN
Sbjct: 4   EFLRTLDRQILLGVFVAFVAVGAGAAYFLTSSK--KRRVCLDPENFKEFKLVKRHQLSHN 63

Query: 62  VAKFTFTLPSPISVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDLGYFELVIKMYPQ 121
           VAKF F LP+  SVLGLPIGQHISCRGKDGQGE+VIKPYTP TLDSD+G FELVIKMYPQ
Sbjct: 64  VAKFVFELPTSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPTTLDSDVGRFELVIKMYPQ 123

Query: 122 GRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPND 181
           GRMSHHFR+MRVGD LAVKGPKGRF+YQPGQ RAFGMLAGGSGITPM+QVARAILENP D
Sbjct: 124 GRMSHHFREMRVGDHLAVKGPKGRFKYQPGQFRAFGMLAGGSGITPMFQVARAILENPTD 183

Query: 182 KTKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQT 241
           KTKVHLIYANVTY+DILLKEEL+ L  +YP+ FK++YVLNQPPE WDGGVGFVSKEMIQT
Sbjct: 184 KTKVHLIYANVTYDDILLKEELEGLTTNYPEQFKIFYVLNQPPEVWDGGVGFVSKEMIQT 243

Query: 242 HCPAPASDIRILRCGPPPMNKAMAAHLEELGYAPEMLFMF 282
           HCPAPASDI+ILRCGPPPMNKAMAA+LE LGY+PEM F F
Sbjct: 244 HCPAPASDIQILRCGPPPMNKAMAANLEALGYSPEMQFQF 281

[Close]

BLAST of Chy8G148070 vs. ExPASy Swiss-Prot
Match: Q54NC1 (NADH-cytochrome b5 reductase 1 OS=Dictyostelium discoideum OX=44689 GN=cyb5r1 PE=3 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 2.2e-74
Identity = 139/280 (49.64%), Postives = 190/280 (67.86%), Query Frame = 0


Query: 15  VAVAVLALAVGALYVL---SSSKKSKSRG----------CLDPEIFKEFKLIKRLQLSHN 74
           V +  +ALA G  YV    S S K K  G           LDP+ +++F+L ++  ++HN
Sbjct: 9   VIIGSVALAAGVKYVFTLTSGSNKDKKGGEAEKGKQVEKALDPQEYRKFQLKEKFIVNHN 68

Query: 75  VAKFTFTLPSPISVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDLGYFELVIKMYPQ 134
              F F LP+   +LGLPIGQHIS R   G G+EV +PYTPI+ D + GYF+L+IK+Y +
Sbjct: 69  TRIFRFALPNEDDILGLPIGQHISLRAVVG-GKEVYRPYTPISSDEERGYFDLLIKVYEK 128

Query: 135 GRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPND 194
           G MS +   M +GD + VKGPKG+F YQP   ++ GMLAGG+GITPM QV +AIL+NP+D
Sbjct: 129 GAMSGYVDNMFIGDSIEVKGPKGKFNYQPNMRKSIGMLAGGTGITPMLQVIKAILKNPSD 188

Query: 195 KTKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQT 254
           KT++ L++ N+T EDILLK+ELD LA+ +P  FK+YYVLN PP+ W  GVGFVSKE+I++
Sbjct: 189 KTEISLVFGNITEEDILLKKELDELAEKHP-QFKVYYVLNNPPKGWTQGVGFVSKEIIES 248

Query: 255 HCPAPASDIRILRCGPPPMNKAMAAHLEELGYAPEMLFMF 282
             P+P+    ++ CGPP MNKAM  HLE +G+    +F F
Sbjct: 249 RLPSPSDQTMVIMCGPPMMNKAMTGHLETIGFNESNIFTF 286

[Close]

BLAST of Chy8G148070 vs. ExPASy Swiss-Prot
Match: Q4X0B5 (NADH-cytochrome b5 reductase 1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=cbr1 PE=3 SV=2)

HSP 1 Score: 271.6 bits (693), Expect = 1.0e-71
Identity = 135/264 (51.14%), Postives = 178/264 (67.42%), Query Frame = 0


Query: 16  AVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSHNVAKFTFTLPSPISV 75
           AVAV A+  G       +K  K    L+P  F+EF L +++ +SHNV  + F LP P  +
Sbjct: 34  AVAVTAILAGLKLFTGGNKPRK---VLNPTEFQEFVLKEKIDVSHNVCIYRFALPRPTDI 93

Query: 76  LGLPIGQHISCRGK-DGQGEEVIKPYTPITLDSDLGYFELVIKMYPQGRMSHHFRQMRVG 135
           LGLPIGQHIS     +GQ +EV++ YTPI+ D++ GYF+L++K YPQG +S +   +++G
Sbjct: 94  LGLPIGQHISLAATIEGQPKEVVRSYTPISSDNEAGYFDLLVKAYPQGNISKYLTTLKIG 153

Query: 136 DFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILEN-----PNDKTKVHLIY 195
           D + V+GPKG   Y P   R  GM+AGG+GITPM Q+ +A++ N      ND TK+ LI+
Sbjct: 154 DTMKVRGPKGAMVYTPNMCRHIGMIAGGTGITPMLQIIKAVIRNRPRNGGNDTTKLDLIF 213

Query: 196 ANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQTHCPAPASD 255
           ANV  +DILLKEELD+LA   PD F +YYVLN PP+ W GGVGFV+ EMI+ H PAPASD
Sbjct: 214 ANVNPDDILLKEELDMLAAEDPD-FNIYYVLNNPPQGWTGGVGFVTPEMIKEHLPAPASD 273

Query: 256 IRILRCGPPPMNKAMAAHLEELGY 274
           ++IL CGPPPM  AM    E LGY
Sbjct: 274 VKILLCGPPPMISAMKKATESLGY 293

[Close]

BLAST of Chy8G148070 vs. ExPASy Swiss-Prot
Match: A5DQ25 (NADH-cytochrome b5 reductase 1 OS=Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324) OX=294746 GN=CBR1 PE=3 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 3.0e-71
Identity = 135/266 (50.75%), Postives = 178/266 (66.92%), Query Frame = 0


Query: 8   DPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSHNVAKFTF 67
           +P ++     A++  AV A+YV+  +KK+     L P++F++F LI++ +LSHN   + F
Sbjct: 7   NPLVIFSTLAAIILAAV-AVYVVKLNKKNGP--VLKPDVFQKFPLIEKTRLSHNTCIYRF 66

Query: 68  TLPSPISVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDLGYFELVIKMYPQGRMSHH 127
            LP     LGLPIGQHIS  G    G+EV++ YTPI+ D +LGYF+L+IK Y QG +S H
Sbjct: 67  GLPKSTDRLGLPIGQHISI-GATINGKEVVRSYTPISRDDELGYFDLLIKTYEQGNISRH 126

Query: 128 FRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPNDKTKVHL 187
                VGD + V+GPKG F Y P  V   GM+AGG+GI PMYQV  AIL NP+DKTK+ L
Sbjct: 127 VDSKSVGDHIEVRGPKGFFTYTPNMVEHLGMIAGGTGIAPMYQVLTAILTNPDDKTKISL 186

Query: 188 IYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQTHCPAPA 247
           +YANVT EDILL+ ELDL AK +PD FK++YVLN  PE W+G VGFV+ E+++ H P   
Sbjct: 187 VYANVTEEDILLRAELDLFAKEHPDRFKVHYVLNNAPENWNGSVGFVTPEIMEKHLPNKD 246

Query: 248 SDIRILRCGPPPMNKAMAAHLEELGY 274
            D  +L CGPPPM  AM  +   LGY
Sbjct: 247 QDGYLLLCGPPPMISAMKKNAVTLGY 268

[Close]

BLAST of Chy8G148070 vs. ExPASy Swiss-Prot
Match: A1DHW1 (NADH-cytochrome b5 reductase 1 OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=cbr1 PE=3 SV=2)

HSP 1 Score: 269.2 bits (687), Expect = 5.1e-71
Identity = 135/264 (51.14%), Postives = 176/264 (66.67%), Query Frame = 0


Query: 16  AVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSHNVAKFTFTLPSPISV 75
           AVA+ A+  G       SK  K    L+P  F+EF L ++  +SHNV  + F LP P  +
Sbjct: 34  AVALTAILAGLKLFTGGSKPRK---VLNPTEFQEFVLKEKTDISHNVCIYRFALPRPADI 93

Query: 76  LGLPIGQHISCRGK-DGQGEEVIKPYTPITLDSDLGYFELVIKMYPQGRMSHHFRQMRVG 135
           LGLPIGQHIS     +GQ +EV++ YTPI+ D++ GYF+L++K YPQG +S +   +++G
Sbjct: 94  LGLPIGQHISLAATIEGQPKEVVRSYTPISSDNEAGYFDLLVKAYPQGNISKYLTTLKIG 153

Query: 136 DFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILEN-----PNDKTKVHLIY 195
           D + V+GPKG   Y P   R  GM+AGG+GITPM Q+ +A++ N      ND TK+ LI+
Sbjct: 154 DTMKVRGPKGAMVYTPNMCRHIGMIAGGTGITPMLQIIKAVIRNRPRNGGNDTTKLDLIF 213

Query: 196 ANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQTHCPAPASD 255
           ANV  +DILLKEELD LA   PD F +YYVLN PP+ W GGVGFV+ EMI+ H PAPASD
Sbjct: 214 ANVNPDDILLKEELDKLAAEDPD-FNIYYVLNNPPQGWTGGVGFVTPEMIKEHLPAPASD 273

Query: 256 IRILRCGPPPMNKAMAAHLEELGY 274
           ++IL CGPPPM  AM    E LGY
Sbjct: 274 VKILLCGPPPMISAMKKATESLGY 293

[Close]

BLAST of Chy8G148070 vs. ExPASy TrEMBL
Match: A0A0A0L226 (FAD-binding FR-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G416480 PE=4 SV=1)

HSP 1 Score: 563.1 bits (1450), Expect = 6.3e-157
Identity = 276/281 (98.22%), Postives = 278/281 (98.93%), Query Frame = 0


Query: 1   MDFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60
           MDFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH
Sbjct: 1   MDFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60

Query: 61  NVAKFTFTLPSPISVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDLGYFELVIKMYP 120
           NVAKFTFTLPSP SVLGLPIGQHISC GKDGQGEEVIKPYTPITLDSD+GYFELVIKMYP
Sbjct: 61  NVAKFTFTLPSPTSVLGLPIGQHISCSGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DKTKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQ 240
           DKTKVHLIYANVTYEDILLKEELDLLAK YPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQ
Sbjct: 181 DKTKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQ 240

Query: 241 THCPAPASDIRILRCGPPPMNKAMAAHLEELGYAPEMLFMF 282
           THCPAPASDI+ILRCGPPPMNKAMAAHLEELGYAPEMLFMF
Sbjct: 241 THCPAPASDIQILRCGPPPMNKAMAAHLEELGYAPEMLFMF 281

[Close]

BLAST of Chy8G148070 vs. ExPASy TrEMBL
Match: A0A5A7TLD5 (NADH--cytochrome b5 reductase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold447G001310 PE=4 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 1.8e-156
Identity = 274/281 (97.51%), Postives = 278/281 (98.93%), Query Frame = 0


Query: 1   MDFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60
           MDFLQSLDPQILLG+AVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH
Sbjct: 1   MDFLQSLDPQILLGLAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60

Query: 61  NVAKFTFTLPSPISVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDLGYFELVIKMYP 120
           NVAKFTFTLP+P SVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSD+GYFELVIKMYP
Sbjct: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DKTKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQ 240
           DKTKVHLIYANVTYEDILLKEELDLLAK YPDSFKLYYVLNQPPE WDGGVGFVSKEMIQ
Sbjct: 181 DKTKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEVWDGGVGFVSKEMIQ 240

Query: 241 THCPAPASDIRILRCGPPPMNKAMAAHLEELGYAPEMLFMF 282
           THCPAPASDI+ILRCGPPPMNKAMAAHLEELGYAPEMLFMF
Sbjct: 241 THCPAPASDIQILRCGPPPMNKAMAAHLEELGYAPEMLFMF 281

[Close]

BLAST of Chy8G148070 vs. ExPASy TrEMBL
Match: A0A1S3C2D1 (NADH--cytochrome b5 reductase 1 OS=Cucumis melo OX=3656 GN=LOC103495874 PE=4 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 1.8e-156
Identity = 274/281 (97.51%), Postives = 278/281 (98.93%), Query Frame = 0


Query: 1   MDFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60
           MDFLQSLDPQILLG+AVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH
Sbjct: 1   MDFLQSLDPQILLGLAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60

Query: 61  NVAKFTFTLPSPISVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDLGYFELVIKMYP 120
           NVAKFTFTLP+P SVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSD+GYFELVIKMYP
Sbjct: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DKTKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQ 240
           DKTKVHLIYANVTYEDILLKEELDLLAK YPDSFKLYYVLNQPPE WDGGVGFVSKEMIQ
Sbjct: 181 DKTKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEVWDGGVGFVSKEMIQ 240

Query: 241 THCPAPASDIRILRCGPPPMNKAMAAHLEELGYAPEMLFMF 282
           THCPAPASDI+ILRCGPPPMNKAMAAHLEELGYAPEMLFMF
Sbjct: 241 THCPAPASDIQILRCGPPPMNKAMAAHLEELGYAPEMLFMF 281

[Close]

BLAST of Chy8G148070 vs. ExPASy TrEMBL
Match: A0A6J1GFP5 (NADH--cytochrome b5 reductase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111453751 PE=4 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 2.3e-154
Identity = 268/281 (95.37%), Postives = 277/281 (98.58%), Query Frame = 0


Query: 1   MDFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60
           MDFLQSLDPQ+LLG+AVAVLALAVG+LYVLSSSKKSKSRGCLDPEIFKEFKLIKR+QLSH
Sbjct: 1   MDFLQSLDPQVLLGLAVAVLALAVGSLYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSH 60

Query: 61  NVAKFTFTLPSPISVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDLGYFELVIKMYP 120
           NVAKFTFTLP+P SVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSD+GYFELVIKMYP
Sbjct: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DKTKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQ 240
           D+TKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPE WDGGVGFVSKEMIQ
Sbjct: 181 DETKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240

Query: 241 THCPAPASDIRILRCGPPPMNKAMAAHLEELGYAPEMLFMF 282
           THCPAPASDI++LRCGPPPMNKAM AHLE LGYAPEMLFMF
Sbjct: 241 THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 281

[Close]

BLAST of Chy8G148070 vs. ExPASy TrEMBL
Match: A0A6J1HVM7 (NADH--cytochrome b5 reductase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111467252 PE=4 SV=1)

HSP 1 Score: 547.4 bits (1409), Expect = 3.6e-152
Identity = 266/281 (94.66%), Postives = 274/281 (97.51%), Query Frame = 0


Query: 1   MDFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60
           MDF QSLDPQILLG+AVA LAL VGALYVLSSSKKSKSRGCLDPEIFKEFKLIKR+QLSH
Sbjct: 1   MDFSQSLDPQILLGLAVAGLALVVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSH 60

Query: 61  NVAKFTFTLPSPISVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDLGYFELVIKMYP 120
           NVAKFTFTLP+P SVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSD+GYFELVIKMYP
Sbjct: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVG+FLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGNFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DKTKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQ 240
           D+TKVHLIYANVTYEDILLKEELDLLAK YPDSFKLYYVLNQPPE WDGGVGFVSKEMIQ
Sbjct: 181 DETKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240

Query: 241 THCPAPASDIRILRCGPPPMNKAMAAHLEELGYAPEMLFMF 282
           THCPAPASDI++LRCGPPPMNKAMAAHLE LGYAPEMLFMF
Sbjct: 241 THCPAPASDIQVLRCGPPPMNKAMAAHLEALGYAPEMLFMF 281

[Close]

BLAST of Chy8G148070 vs. NCBI nr
Match: XP_004144304.1 (NADH--cytochrome b5 reductase 1 [Cucumis sativus] >KGN54652.1 hypothetical protein Csa_012318 [Cucumis sativus])

HSP 1 Score: 559 bits (1441), Expect = 7.45e-201
Identity = 276/281 (98.22%), Postives = 278/281 (98.93%), Query Frame = 0


Query: 1   MDFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60
           MDFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH
Sbjct: 1   MDFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60

Query: 61  NVAKFTFTLPSPISVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDLGYFELVIKMYP 120
           NVAKFTFTLPSP SVLGLPIGQHISC GKDGQGEEVIKPYTPITLDSD+GYFELVIKMYP
Sbjct: 61  NVAKFTFTLPSPTSVLGLPIGQHISCSGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DKTKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQ 240
           DKTKVHLIYANVTYEDILLKEELDLLAK YPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQ
Sbjct: 181 DKTKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQ 240

Query: 241 THCPAPASDIRILRCGPPPMNKAMAAHLEELGYAPEMLFMF 281
           THCPAPASDI+ILRCGPPPMNKAMAAHLEELGYAPEMLFMF
Sbjct: 241 THCPAPASDIQILRCGPPPMNKAMAAHLEELGYAPEMLFMF 281

[Close]

BLAST of Chy8G148070 vs. NCBI nr
Match: XP_008455773.1 (PREDICTED: NADH--cytochrome b5 reductase 1 [Cucumis melo] >KAA0043648.1 NADH--cytochrome b5 reductase 1 [Cucumis melo var. makuwa] >TYK09714.1 NADH--cytochrome b5 reductase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 558 bits (1437), Expect = 3.04e-200
Identity = 274/281 (97.51%), Postives = 278/281 (98.93%), Query Frame = 0


Query: 1   MDFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60
           MDFLQSLDPQILLG+AVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH
Sbjct: 1   MDFLQSLDPQILLGLAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60

Query: 61  NVAKFTFTLPSPISVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDLGYFELVIKMYP 120
           NVAKFTFTLP+P SVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSD+GYFELVIKMYP
Sbjct: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DKTKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQ 240
           DKTKVHLIYANVTYEDILLKEELDLLAK YPDSFKLYYVLNQPPE WDGGVGFVSKEMIQ
Sbjct: 181 DKTKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEVWDGGVGFVSKEMIQ 240

Query: 241 THCPAPASDIRILRCGPPPMNKAMAAHLEELGYAPEMLFMF 281
           THCPAPASDI+ILRCGPPPMNKAMAAHLEELGYAPEMLFMF
Sbjct: 241 THCPAPASDIQILRCGPPPMNKAMAAHLEELGYAPEMLFMF 281

[Close]

BLAST of Chy8G148070 vs. NCBI nr
Match: XP_022950742.1 (NADH--cytochrome b5 reductase 1-like [Cucurbita moschata])

HSP 1 Score: 551 bits (1419), Expect = 1.68e-197
Identity = 268/281 (95.37%), Postives = 277/281 (98.58%), Query Frame = 0


Query: 1   MDFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60
           MDFLQSLDPQ+LLG+AVAVLALAVG+LYVLSSSKKSKSRGCLDPEIFKEFKLIKR+QLSH
Sbjct: 1   MDFLQSLDPQVLLGLAVAVLALAVGSLYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSH 60

Query: 61  NVAKFTFTLPSPISVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDLGYFELVIKMYP 120
           NVAKFTFTLP+P SVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSD+GYFELVIKMYP
Sbjct: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DKTKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQ 240
           D+TKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPE WDGGVGFVSKEMIQ
Sbjct: 181 DETKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240

Query: 241 THCPAPASDIRILRCGPPPMNKAMAAHLEELGYAPEMLFMF 281
           THCPAPASDI++LRCGPPPMNKAM AHLE LGYAPEMLFMF
Sbjct: 241 THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 281

[Close]

BLAST of Chy8G148070 vs. NCBI nr
Match: KAG7033625.1 (NADH--cytochrome b5 reductase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 549 bits (1414), Expect = 9.74e-197
Identity = 267/281 (95.02%), Postives = 276/281 (98.22%), Query Frame = 0


Query: 1   MDFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60
           MDFLQSLDPQ+LLG+AVAVLALAVG+LYVLSSSKKSKSRGCLDPEIFKEFKLIKR+QLSH
Sbjct: 1   MDFLQSLDPQVLLGLAVAVLALAVGSLYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSH 60

Query: 61  NVAKFTFTLPSPISVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDLGYFELVIKMYP 120
           NVAKFTFTLP+P SVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSD+GYFELVIKMYP
Sbjct: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DKTKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQ 240
           D+TKVHLIYANVTYEDILLKEELDLLAK YPDSFKLYYVLNQPPE WDGGVGFVSKEMIQ
Sbjct: 181 DETKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240

Query: 241 THCPAPASDIRILRCGPPPMNKAMAAHLEELGYAPEMLFMF 281
           THCPAPASDI++LRCGPPPMNKAM AHLE LGYAPEMLFMF
Sbjct: 241 THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 281

[Close]

BLAST of Chy8G148070 vs. NCBI nr
Match: KAG6603441.1 (NADH--cytochrome b5 reductase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 547 bits (1410), Expect = 3.97e-196
Identity = 266/281 (94.66%), Postives = 275/281 (97.86%), Query Frame = 0


Query: 1   MDFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60
           MDFLQSLDPQ+LLG+AVAVLALAVG+LYVLSSSKKSKSRGCLDPEIFKEFKLIKR+QLSH
Sbjct: 1   MDFLQSLDPQVLLGLAVAVLALAVGSLYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSH 60

Query: 61  NVAKFTFTLPSPISVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDLGYFELVIKMYP 120
           NVAKFTFTLP+P SVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSD+GYFELVIKMYP
Sbjct: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMR GDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRAGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DKTKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQ 240
           D+TKVHLIYANVTYEDILLKEELDLLAK YPDSFKLYYVLNQPPE WDGGVGFVSKEMIQ
Sbjct: 181 DETKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240

Query: 241 THCPAPASDIRILRCGPPPMNKAMAAHLEELGYAPEMLFMF 281
           THCPAPASDI++LRCGPPPMNKAM AHLE LGYAPEMLFMF
Sbjct: 241 THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 281

[Close]

BLAST of Chy8G148070 vs. TAIR 10
Match: AT5G17770.1 (NADH:cytochrome B5 reductase 1 )

HSP 1 Score: 469.9 bits (1208), Expect = 1.4e-132
Identity = 228/280 (81.43%), Postives = 250/280 (89.29%), Query Frame = 0


Query: 2   DFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSHN 61
           +FL++LD QILLGV VA +A+  GA Y L+SSK  K R CLDPE FKEFKL+KR QLSHN
Sbjct: 4   EFLRTLDRQILLGVFVAFVAVGAGAAYFLTSSK--KRRVCLDPENFKEFKLVKRHQLSHN 63

Query: 62  VAKFTFTLPSPISVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDLGYFELVIKMYPQ 121
           VAKF F LP+  SVLGLPIGQHISCRGKDGQGE+VIKPYTP TLDSD+G FELVIKMYPQ
Sbjct: 64  VAKFVFELPTSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPTTLDSDVGRFELVIKMYPQ 123

Query: 122 GRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPND 181
           GRMSHHFR+MRVGD LAVKGPKGRF+YQPGQ RAFGMLAGGSGITPM+QVARAILENP D
Sbjct: 124 GRMSHHFREMRVGDHLAVKGPKGRFKYQPGQFRAFGMLAGGSGITPMFQVARAILENPTD 183

Query: 182 KTKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQT 241
           KTKVHLIYANVTY+DILLKEEL+ L  +YP+ FK++YVLNQPPE WDGGVGFVSKEMIQT
Sbjct: 184 KTKVHLIYANVTYDDILLKEELEGLTTNYPEQFKIFYVLNQPPEVWDGGVGFVSKEMIQT 243

Query: 242 HCPAPASDIRILRCGPPPMNKAMAAHLEELGYAPEMLFMF 282
           HCPAPASDI+ILRCGPPPMNKAMAA+LE LGY+PEM F F
Sbjct: 244 HCPAPASDIQILRCGPPPMNKAMAANLEALGYSPEMQFQF 281

[Close]

BLAST of Chy8G148070 vs. TAIR 10
Match: AT5G20080.1 (FAD/NAD(P)-binding oxidoreductase )

HSP 1 Score: 216.5 bits (550), Expect = 2.8e-56
Identity = 111/273 (40.66%), Postives = 168/273 (61.54%), Query Frame = 0


Query: 27  LYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSHNVAKFTFTLPSPISVLGLPIGQHISC 86
           +Y+  + +++  +  L+P+ + EFKL    ++SHN   F F+   P + LGL +   +  
Sbjct: 58  VYLDQAKEETGPKTALNPDKWLEFKLQDTARVSHNTQLFRFSF-DPSAELGLHVASCLLT 117

Query: 87  R-----GKDGQGEEVIKPYTPITLDSDLGYFELVIKMYPQGRMSHHFRQMRVGDFLAVKG 146
           R       +G+ + VI+PYTPI+     GYF+L+IK+YP G+MS HF  ++ GD L VKG
Sbjct: 118 RAPLGYNAEGKTKYVIRPYTPISDPEAKGYFDLLIKVYPDGKMSQHFASLKPGDVLEVKG 177

Query: 147 PKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPNDKTKVHLIYANVTYEDILLKE 206
           P  +F+Y P   +  GM+AGGSGITPM QV  AI++NP D T++ L+YANV+ +DILLK+
Sbjct: 178 PVEKFKYSPNMKKHIGMIAGGSGITPMLQVIDAIVKNPEDNTQISLLYANVSPDDILLKQ 237

Query: 207 ELDLLAKSYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQTHCPAPASDIRILRCGPPPMN 266
           +LD+L  ++P + K++Y ++ P + W GGVG++SK+M     P P  D  IL CGPP M 
Sbjct: 238 KLDVLQANHP-NLKIFYTVDNPTKNWKGGVGYISKDMALKGLPLPTDDTLILVCGPPGMM 297

Query: 267 KAMAAH-------------LEELGYAPEMLFMF 282
           + ++               L+ELGY  EM+F F
Sbjct: 298 EHISGGKAPDWSQGEVKGILKELGYTEEMVFKF 328

[Close]

BLAST of Chy8G148070 vs. TAIR 10
Match: AT1G37130.1 (nitrate reductase 2 )

HSP 1 Score: 174.1 bits (440), Expect = 1.6e-43
Identity = 96/253 (37.94%), Postives = 147/253 (58.10%), Query Frame = 0


Query: 42  LDPEIFKEFKLIKRLQLSHNVAKFTFTLPSPISVLGLPIGQHISCRGKDGQGEEVIKPYT 101
           ++P      +L+++  +SH+V KF F LP    VLGLP+G+HI         +  ++ YT
Sbjct: 657 VNPRAKVPVQLVEKTSISHDVRKFRFALPVEDMVLGLPVGKHIFLCATIND-KLCLRAYT 716

Query: 102 PITLDSDLGYFELVIKMY-----PQ----GRMSHHFRQMRVGDFLAVKGPKGRFRY---- 161
           P +    +GYFELV+K+Y     P+    G MS +   + +G  L +KGP G   Y    
Sbjct: 717 PSSTVDVVGYFELVVKIYFGGVHPRFPNGGLMSQYLDSLPIGSTLEIKGPLGHVEYLGKG 776

Query: 162 ------QPGQVRAFGMLAGGSGITPMYQVARAILENPNDKTKVHLIYANVTYEDILLKEE 221
                 +P       MLAGG+GITP+YQ+ +AIL++P D+T++++IYAN T EDILL+EE
Sbjct: 777 SFTVHGKPKFADKLAMLAGGTGITPVYQIIQAILKDPEDETEMYVIYANRTEEDILLREE 836

Query: 222 LDLLAKSYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQTHCPAPASDIRI-LRCGPPPMN 274
           LD  A+ YPD  K++YV+    E W    GF+S+ +++ H P       + + CGPPPM 
Sbjct: 837 LDGWAEQYPDRLKVWYVVESAKEGWAYSTGFISEAIMREHIPDGLDGSALAMACGPPPMI 896

[Close]

BLAST of Chy8G148070 vs. TAIR 10
Match: AT1G77760.1 (nitrate reductase 1 )

HSP 1 Score: 164.9 bits (416), Expect = 9.7e-41
Identity = 85/244 (34.84%), Postives = 142/244 (58.20%), Query Frame = 0


Query: 51  KLIKRLQLSHNVAKFTFTLPSPISVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDLG 110
           +LI++  +SH+V KF F LPS    LGLP+G+H+         +  ++ YTP +    +G
Sbjct: 666 RLIEKTSISHDVRKFRFALPSEDQQLGLPVGKHVFVCANIND-KLCLRAYTPTSAIDAVG 725

Query: 111 YFELVIKMY-----PQ----GRMSHHFRQMRVGDFLAVKGPKGRFRY----------QPG 170
           + +LV+K+Y     P+    G MS H   + +G  + +KGP G   Y          +P 
Sbjct: 726 HIDLVVKVYFKDVHPRFPNGGLMSQHLDSLPIGSMIDIKGPLGHIEYKGKGNFLVSGKPK 785

Query: 171 QVRAFGMLAGGSGITPMYQVARAILENPNDKTKVHLIYANVTYEDILLKEELDLLAKSYP 230
             +   MLAGG+GITP+YQ+ ++IL +P D+T+++++YAN T +DIL++EEL+  A  + 
Sbjct: 786 FAKKLAMLAGGTGITPIYQIIQSILSDPEDETEMYVVYANRTEDDILVREELEGWASKHK 845

Query: 231 DSFKLYYVLNQPPEAWDGGVGFVSKEMIQTHCP-APASDIRILRCGPPPMNK-AMAAHLE 274
           +  K++YV+    E W    GF+++ +++ H P     +   L CGPPPM + A+  +LE
Sbjct: 846 ERLKIWYVVEIAKEGWSYSTGFITEAVLREHIPEGLEGESLALACGPPPMIQFALQPNLE 905

The following BLAST results are available for this feature:

BLAST of Chy8G148070 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZNT1	2.0e-131	81.43	NADH--cytochrome b5 reductase 1 OS=Arabidopsis thaliana OX=3702 GN=CBR1 PE=1 SV=...	[more]
Q54NC1	2.2e-74	49.64	NADH-cytochrome b5 reductase 1 OS=Dictyostelium discoideum OX=44689 GN=cyb5r1 PE...	[more]
Q4X0B5	1.0e-71	51.14	NADH-cytochrome b5 reductase 1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / A...	[more]
A5DQ25	3.0e-71	50.75	NADH-cytochrome b5 reductase 1 OS=Meyerozyma guilliermondii (strain ATCC 6260 / ...	[more]
A1DHW1	5.1e-71	51.14	NADH-cytochrome b5 reductase 1 OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3...	[more]

BLAST of Chy8G148070 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L226	6.3e-157	98.22	FAD-binding FR-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_...	[more]
A0A5A7TLD5	1.8e-156	97.51	NADH--cytochrome b5 reductase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A1S3C2D1	1.8e-156	97.51	NADH--cytochrome b5 reductase 1 OS=Cucumis melo OX=3656 GN=LOC103495874 PE=4 SV=...	[more]
A0A6J1GFP5	2.3e-154	95.37	NADH--cytochrome b5 reductase 1-like OS=Cucurbita moschata OX=3662 GN=LOC1114537...	[more]
A0A6J1HVM7	3.6e-152	94.66	NADH--cytochrome b5 reductase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111467252...	[more]

BLAST of Chy8G148070 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004144304.1	7.45e-201	98.22	NADH--cytochrome b5 reductase 1 [Cucumis sativus] >KGN54652.1 hypothetical prote...	[more]
XP_008455773.1	3.04e-200	97.51	PREDICTED: NADH--cytochrome b5 reductase 1 [Cucumis melo] >KAA0043648.1 NADH--cy...	[more]
XP_022950742.1	1.68e-197	95.37	NADH--cytochrome b5 reductase 1-like [Cucurbita moschata]	[more]
KAG7033625.1	9.74e-197	95.02	NADH--cytochrome b5 reductase 1 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6603441.1	3.97e-196	94.66	NADH--cytochrome b5 reductase 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Chy8G148070 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G17770.1	1.4e-132	81.43	NADH:cytochrome B5 reductase 1	[more]
AT5G20080.1	2.8e-56	40.66	FAD/NAD(P)-binding oxidoreductase	[more]
AT1G37130.1	1.6e-43	37.94	nitrate reductase 2	[more]
AT1G77760.1	9.7e-41	34.84	nitrate reductase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001834	NADH:cytochrome b5 reductase-like	PRINTS	PR00406	CYTB5RDTASE	coord: 157..176 score: 66.67 coord: 134..148 score: 49.31 coord: 76..87 score: 57.47 coord: 98..105 score: 41.41 coord: 251..259 score: 59.26 coord: 195..206 score: 64.06
IPR008333	Flavoprotein pyridine nucleotide cytochrome reductase-like, FAD-binding domain	PFAM	PF00970	FAD_binding_6	coord: 50..148 e-value: 6.2E-31 score: 106.7
IPR039261	Ferredoxin-NADP reductase (FNR), nucleotide-binding domain	GENE3D	3.40.50.80		coord: 140..281 e-value: 1.5E-44 score: 153.3
IPR039261	Ferredoxin-NADP reductase (FNR), nucleotide-binding domain	SUPERFAMILY	52343	Ferredoxin reductase-like, C-terminal NADP-linked domain	coord: 137..278
None	No IPR available	GENE3D	2.40.30.10	Translation factors	coord: 40..139 e-value: 9.7E-33 score: 114.8
None	No IPR available	PANTHER	PTHR19370	NADH-CYTOCHROME B5 REDUCTASE	coord: 2..281
None	No IPR available	PANTHER	PTHR19370:SF203	NADH-CYTOCHROME B5 REDUCTASE	coord: 2..281
None	No IPR available	CDD	cd06183	cyt_b5_reduct_like	coord: 50..281 e-value: 3.6101E-117 score: 334.15
IPR001433	Oxidoreductase FAD/NAD(P)-binding	PFAM	PF00175	NAD_binding_1	coord: 158..264 e-value: 4.7E-30 score: 104.4
IPR017927	FAD-binding domain, ferredoxin reductase-type	PROSITE	PS51384	FAD_FR	coord: 45..149 score: 13.82414
IPR017938	Riboflavin synthase-like beta-barrel	SUPERFAMILY	63380	Riboflavin synthase domain-like	coord: 42..150

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy8G148070.1	Chy8G148070.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0022900	electron transport chain
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004128	cytochrome-b5 reductase activity, acting on NAD(P)H
molecular_function	GO:0009703	nitrate reductase (NADH) activity
molecular_function	GO:0016491	oxidoreductase activity