Chy8G147730 (gene) Cucumber (hystrix) v1

Overview


Name	Chy8G147730
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	P-loop containing nucleoside triphosphate hydrolase
Location	chrH08: 3781555 .. 3781827 (-)
RNA-Seq Expression	Chy8G147730
Synteny	Chy8G147730

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTATGTCCTTTCTGTTTCTTCGATTTACATGAAGGGAAAGAATCTCAGCCCCCAGGAAGTGGATCTTGGGTGAACGTTGATGAGGCTGATTTTGTCCTCCACCTTTATCATAAGTTGGTTATTTCATATCCAGAGTTGAAGTCAAATTCTCAAGTTGCAATCATATCACCCTATAACCAGCAAGTAAAACTTCTCCAAGAAAAGTTTGTGGATCTGTTTGGAATGGATCCTAGTGGCATAATAGATATTACAAGTGTTGACGGTTGCCAG

mRNA sequence

ATGTTATGTCCTTTCTGTTTCTTCGATTTACATGAAGGGAAAGAATCTCAGCCCCCAGGAAGTGGATCTTGGGTGAACGTTGATGAGGCTGATTTTGTCCTCCACCTTTATCATAAGTTGGTTATTTCATATCCAGAGTTGAAGTCAAATTCTCAAGTTGCAATCATATCACCCTATAACCAGCAAGTAAAACTTCTCCAAGAAAAGTTTGTGGATCTGTTTGGAATGGATCCTAGTGGCATAATAGATATTACAAGTGTTGACGGTTGCCAG

Coding sequence (CDS)

ATGTTATGTCCTTTCTGTTTCTTCGATTTACATGAAGGGAAAGAATCTCAGCCCCCAGGAAGTGGATCTTGGGTGAACGTTGATGAGGCTGATTTTGTCCTCCACCTTTATCATAAGTTGGTTATTTCATATCCAGAGTTGAAGTCAAATTCTCAAGTTGCAATCATATCACCCTATAACCAGCAAGTAAAACTTCTCCAAGAAAAGTTTGTGGATCTGTTTGGAATGGATCCTAGTGGCATAATAGATATTACAAGTGTTGACGGTTGCCAG

Protein sequence

MLCPFCFFDLHEGKESQPPGSGSWVNVDEADFVLHLYHKLVISYPELKSNSQVAIISPYNQQVKLLQEKFVDLFGMDPSGIIDITSVDGCQ

Homology

[Close]

BLAST of Chy8G147730 vs. ExPASy Swiss-Prot
Match: B6SFA4 (Probable helicase MAGATAMA 3 OS=Arabidopsis thaliana OX=3702 GN=MAA3 PE=2 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 2.5e-27
Identity = 55/89 (61.80%), Postives = 72/89 (80.90%), Query Frame = 0


Query: 4   PFCFFDLHEGKESQPPG-SGSWVNVDEADFVLHLYHKLVISYPELKSNSQVAIISPYNQQ 63
           PFCFFD+HEGKESQ PG +GS VN+DE +FVL +YH+LV  YPELKS+SQ+AIISPYN Q
Sbjct: 597 PFCFFDIHEGKESQHPGATGSRVNLDEVEFVLLIYHRLVTMYPELKSSSQLAIISPYNYQ 656

Query: 64  VKLLQEKFVDLFGMDPSGIIDITSVDGCQ 92
           VK  +++F ++FG +   ++DI +VDG Q
Sbjct: 657 VKTFKDRFKEMFGTEAEKVVDINTVDGFQ 685

[Close]

BLAST of Chy8G147730 vs. ExPASy Swiss-Prot
Match: O94387 (Uncharacterized ATP-dependent helicase C29A10.10c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC29A10.10c PE=3 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 4.9e-07
Identity = 30/85 (35.29%), Postives = 49/85 (57.65%), Query Frame = 0


Query: 7    FFDLHEGKESQPPGSGSWVNVDEADFVLHLYHKLVISYPELKSNSQVAIISPYNQQVKLL 66
            FF++H G E+    S S  NV+EA F+L LY +L+  Y  +    ++ +++PY  QV+ L
Sbjct: 1627 FFNVH-GTEAF-SNSKSLYNVEEASFILLLYERLIQCYLNIDFEGKIGVVTPYRSQVQQL 1686

Query: 67   QEKFVDLFGMDPSGIIDITSVDGCQ 92
            + +F   +G      +DI +VDG Q
Sbjct: 1687 RSQFQRKYGSIIFKHLDIHTVDGFQ 1709

[Close]

BLAST of Chy8G147730 vs. ExPASy TrEMBL
Match: A0A5A7UB64 (Putative helicase MAGATAMA 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2510G00490 PE=4 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 4.0e-44
Identity = 86/88 (97.73%), Postives = 88/88 (100.00%), Query Frame = 0


Query: 4   PFCFFDLHEGKESQPPGSGSWVNVDEADFVLHLYHKLVISYPELKSNSQVAIISPYNQQV 63
           PFCFFDLHEGKESQPPGSGSWVNVDEADFVLHLYHKLVISYPELKSNSQVAIISPY+QQV
Sbjct: 602 PFCFFDLHEGKESQPPGSGSWVNVDEADFVLHLYHKLVISYPELKSNSQVAIISPYSQQV 661

Query: 64  KLLQEKFVDLFGMDPSGIIDITSVDGCQ 92
           KLLQEKFVDLFGMDPSGI+DITSVDGCQ
Sbjct: 662 KLLQEKFVDLFGMDPSGIVDITSVDGCQ 689

[Close]

BLAST of Chy8G147730 vs. ExPASy TrEMBL
Match: A0A1S3CEF6 (probable helicase MAGATAMA 3 OS=Cucumis melo OX=3656 GN=LOC103499555 PE=4 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 4.0e-44
Identity = 86/88 (97.73%), Postives = 88/88 (100.00%), Query Frame = 0


Query: 4   PFCFFDLHEGKESQPPGSGSWVNVDEADFVLHLYHKLVISYPELKSNSQVAIISPYNQQV 63
           PFCFFDLHEGKESQPPGSGSWVNVDEADFVLHLYHKLVISYPELKSNSQVAIISPY+QQV
Sbjct: 602 PFCFFDLHEGKESQPPGSGSWVNVDEADFVLHLYHKLVISYPELKSNSQVAIISPYSQQV 661

Query: 64  KLLQEKFVDLFGMDPSGIIDITSVDGCQ 92
           KLLQEKFVDLFGMDPSGI+DITSVDGCQ
Sbjct: 662 KLLQEKFVDLFGMDPSGIVDITSVDGCQ 689

[Close]

BLAST of Chy8G147730 vs. ExPASy TrEMBL
Match: A0A0A0KM05 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G523480 PE=4 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 5.2e-44
Identity = 85/88 (96.59%), Postives = 88/88 (100.00%), Query Frame = 0


Query: 4   PFCFFDLHEGKESQPPGSGSWVNVDEADFVLHLYHKLVISYPELKSNSQVAIISPYNQQV 63
           PFCFFDLHEGKESQPPGSGSWVN+DEADFVLHLYHKLVISYPELKSNSQVAIISPY+QQV
Sbjct: 602 PFCFFDLHEGKESQPPGSGSWVNIDEADFVLHLYHKLVISYPELKSNSQVAIISPYSQQV 661

Query: 64  KLLQEKFVDLFGMDPSGIIDITSVDGCQ 92
           KLLQEKFVDLFGMDPSGI+DITSVDGCQ
Sbjct: 662 KLLQEKFVDLFGMDPSGIVDITSVDGCQ 689

[Close]

BLAST of Chy8G147730 vs. ExPASy TrEMBL
Match: A0A6J1J637 (probable helicase MAGATAMA 3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482100 PE=4 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 9.2e-41
Identity = 81/88 (92.05%), Postives = 85/88 (96.59%), Query Frame = 0


Query: 4   PFCFFDLHEGKESQPPGSGSWVNVDEADFVLHLYHKLVISYPELKSNSQVAIISPYNQQV 63
           PFCFFDLHEGKESQPPGSGSWVNVDEA+FVL LYHKLVISYPELKSNSQVAIISPY+QQV
Sbjct: 602 PFCFFDLHEGKESQPPGSGSWVNVDEAEFVLLLYHKLVISYPELKSNSQVAIISPYSQQV 661

Query: 64  KLLQEKFVDLFGMDPSGIIDITSVDGCQ 92
           KLLQEKF D FG+DPSGI+DITSVDGCQ
Sbjct: 662 KLLQEKFKDTFGLDPSGIVDITSVDGCQ 689

[Close]

BLAST of Chy8G147730 vs. ExPASy TrEMBL
Match: A0A6J1J2J7 (probable helicase MAGATAMA 3 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111482100 PE=4 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 9.2e-41
Identity = 81/88 (92.05%), Postives = 85/88 (96.59%), Query Frame = 0


Query: 4   PFCFFDLHEGKESQPPGSGSWVNVDEADFVLHLYHKLVISYPELKSNSQVAIISPYNQQV 63
           PFCFFDLHEGKESQPPGSGSWVNVDEA+FVL LYHKLVISYPELKSNSQVAIISPY+QQV
Sbjct: 551 PFCFFDLHEGKESQPPGSGSWVNVDEAEFVLLLYHKLVISYPELKSNSQVAIISPYSQQV 610

Query: 64  KLLQEKFVDLFGMDPSGIIDITSVDGCQ 92
           KLLQEKF D FG+DPSGI+DITSVDGCQ
Sbjct: 611 KLLQEKFKDTFGLDPSGIVDITSVDGCQ 638

[Close]

BLAST of Chy8G147730 vs. NCBI nr
Match: XP_008460794.1 (PREDICTED: probable helicase MAGATAMA 3 [Cucumis melo] >KAA0053183.1 putative helicase MAGATAMA 3 [Cucumis melo var. makuwa] >TYK00157.1 putative helicase MAGATAMA 3 [Cucumis melo var. makuwa])

HSP 1 Score: 184 bits (468), Expect = 4.13e-52
Identity = 86/88 (97.73%), Postives = 88/88 (100.00%), Query Frame = 0


Query: 4   PFCFFDLHEGKESQPPGSGSWVNVDEADFVLHLYHKLVISYPELKSNSQVAIISPYNQQV 63
           PFCFFDLHEGKESQPPGSGSWVNVDEADFVLHLYHKLVISYPELKSNSQVAIISPY+QQV
Sbjct: 602 PFCFFDLHEGKESQPPGSGSWVNVDEADFVLHLYHKLVISYPELKSNSQVAIISPYSQQV 661

Query: 64  KLLQEKFVDLFGMDPSGIIDITSVDGCQ 91
           KLLQEKFVDLFGMDPSGI+DITSVDGCQ
Sbjct: 662 KLLQEKFVDLFGMDPSGIVDITSVDGCQ 689

[Close]

BLAST of Chy8G147730 vs. NCBI nr
Match: XP_011658296.1 (probable helicase MAGATAMA 3 [Cucumis sativus] >KGN49407.1 hypothetical protein Csa_002757 [Cucumis sativus])

HSP 1 Score: 184 bits (467), Expect = 5.70e-52
Identity = 85/88 (96.59%), Postives = 88/88 (100.00%), Query Frame = 0


Query: 4   PFCFFDLHEGKESQPPGSGSWVNVDEADFVLHLYHKLVISYPELKSNSQVAIISPYNQQV 63
           PFCFFDLHEGKESQPPGSGSWVN+DEADFVLHLYHKLVISYPELKSNSQVAIISPY+QQV
Sbjct: 602 PFCFFDLHEGKESQPPGSGSWVNIDEADFVLHLYHKLVISYPELKSNSQVAIISPYSQQV 661

Query: 64  KLLQEKFVDLFGMDPSGIIDITSVDGCQ 91
           KLLQEKFVDLFGMDPSGI+DITSVDGCQ
Sbjct: 662 KLLQEKFVDLFGMDPSGIVDITSVDGCQ 689

[Close]

BLAST of Chy8G147730 vs. NCBI nr
Match: XP_022935507.1 (probable helicase MAGATAMA 3 isoform X2 [Cucurbita moschata])

HSP 1 Score: 173 bits (439), Expect = 2.91e-48
Identity = 81/88 (92.05%), Postives = 85/88 (96.59%), Query Frame = 0


Query: 4   PFCFFDLHEGKESQPPGSGSWVNVDEADFVLHLYHKLVISYPELKSNSQVAIISPYNQQV 63
           PFCFFDLHEGKESQPPGSGSWVNVDEA+FVL LYHKLVISYPELKSNSQVAIISPY+QQV
Sbjct: 527 PFCFFDLHEGKESQPPGSGSWVNVDEAEFVLLLYHKLVISYPELKSNSQVAIISPYSQQV 586

Query: 64  KLLQEKFVDLFGMDPSGIIDITSVDGCQ 91
           KLLQEKF D FG+DPSGI+DITSVDGCQ
Sbjct: 587 KLLQEKFKDTFGLDPSGIVDITSVDGCQ 614

[Close]

BLAST of Chy8G147730 vs. NCBI nr
Match: XP_023528759.1 (probable helicase MAGATAMA 3 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 173 bits (439), Expect = 2.93e-48
Identity = 81/88 (92.05%), Postives = 85/88 (96.59%), Query Frame = 0


Query: 4   PFCFFDLHEGKESQPPGSGSWVNVDEADFVLHLYHKLVISYPELKSNSQVAIISPYNQQV 63
           PFCFFDLHEGKESQPPGSGSWVNVDEA+FVL LYHKLVISYPELKSNSQVAIISPY+QQV
Sbjct: 527 PFCFFDLHEGKESQPPGSGSWVNVDEAEFVLLLYHKLVISYPELKSNSQVAIISPYSQQV 586

Query: 64  KLLQEKFVDLFGMDPSGIIDITSVDGCQ 91
           KLLQEKF D FG+DPSGI+DITSVDGCQ
Sbjct: 587 KLLQEKFKDTFGLDPSGIVDITSVDGCQ 614

[Close]

BLAST of Chy8G147730 vs. NCBI nr
Match: XP_022983526.1 (probable helicase MAGATAMA 3 isoform X2 [Cucurbita maxima])

HSP 1 Score: 173 bits (439), Expect = 3.42e-48
Identity = 81/88 (92.05%), Postives = 85/88 (96.59%), Query Frame = 0


Query: 4   PFCFFDLHEGKESQPPGSGSWVNVDEADFVLHLYHKLVISYPELKSNSQVAIISPYNQQV 63
           PFCFFDLHEGKESQPPGSGSWVNVDEA+FVL LYHKLVISYPELKSNSQVAIISPY+QQV
Sbjct: 551 PFCFFDLHEGKESQPPGSGSWVNVDEAEFVLLLYHKLVISYPELKSNSQVAIISPYSQQV 610

Query: 64  KLLQEKFVDLFGMDPSGIIDITSVDGCQ 91
           KLLQEKF D FG+DPSGI+DITSVDGCQ
Sbjct: 611 KLLQEKFKDTFGLDPSGIVDITSVDGCQ 638

[Close]

BLAST of Chy8G147730 vs. TAIR 10
Match: AT4G15570.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 122.5 bits (306), Expect = 1.8e-28
Identity = 55/89 (61.80%), Postives = 72/89 (80.90%), Query Frame = 0


Query: 4   PFCFFDLHEGKESQPPG-SGSWVNVDEADFVLHLYHKLVISYPELKSNSQVAIISPYNQQ 63
           PFCFFD+HEGKESQ PG +GS VN+DE +FVL +YH+LV  YPELKS+SQ+AIISPYN Q
Sbjct: 597 PFCFFDIHEGKESQHPGATGSRVNLDEVEFVLLIYHRLVTMYPELKSSSQLAIISPYNYQ 656

Query: 64  VKLLQEKFVDLFGMDPSGIIDITSVDGCQ 92
           VK  +++F ++FG +   ++DI +VDG Q
Sbjct: 657 VKTFKDRFKEMFGTEAEKVVDINTVDGFQ 685

[Close]

BLAST of Chy8G147730 vs. TAIR 10
Match: AT2G19120.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 60.8 bits (146), Expect = 6.4e-10
Identity = 34/92 (36.96%), Postives = 51/92 (55.43%), Query Frame = 0


Query: 1   MLCPFCFFDLHEGKESQPPGSGSWVNVDEADFVLHLYHKLVISYPELKSNS-QVAIISPY 60
           +L P+ FF++  G+ES   GS S+ NVDEA F + +Y  L  +   L +    V +I+PY
Sbjct: 885 VLRPYLFFNISHGRESHRGGSVSYENVDEARFCVGVYMHLQKTLKSLGAGKVSVGVITPY 944

Query: 61  NQQVKLLQEKFVDLFGMDPSGIIDITSVDGCQ 92
             Q+K L+ +F +  G D    I I +VD  Q
Sbjct: 945 KLQLKCLKHEFGNALGQDELKEIYINTVDAFQ 976

[Close]

BLAST of Chy8G147730 vs. TAIR 10
Match: AT4G30100.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 58.2 bits (139), Expect = 4.1e-09
Identity = 33/92 (35.87%), Postives = 49/92 (53.26%), Query Frame = 0


Query: 1    MLCPFCFFDLHEGKESQPPGSGSWVNVDEADFVLHLYHKLVISYPELKSNS-QVAIISPY 60
            +L P+ FFD+  G+ES   GS S+ N+DEA F + +Y  L  +   L      V +I+PY
Sbjct: 1048 VLKPYLFFDISHGRESHRGGSVSYENIDEARFCVGVYLHLQRTLKSLGGGKVSVGVITPY 1107

Query: 61   NQQVKLLQEKFVDLFGMDPSGIIDITSVDGCQ 92
              Q+K L+ +F +    D    I I +VD  Q
Sbjct: 1108 KLQLKCLKIEFGNALSQDELQEIYINTVDAFQ 1139

The following BLAST results are available for this feature:

BLAST of Chy8G147730 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B6SFA4	2.5e-27	61.80	Probable helicase MAGATAMA 3 OS=Arabidopsis thaliana OX=3702 GN=MAA3 PE=2 SV=1	[more]
O94387	4.9e-07	35.29	Uncharacterized ATP-dependent helicase C29A10.10c OS=Schizosaccharomyces pombe (...	[more]

BLAST of Chy8G147730 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UB64	4.0e-44	97.73	Putative helicase MAGATAMA 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A1S3CEF6	4.0e-44	97.73	probable helicase MAGATAMA 3 OS=Cucumis melo OX=3656 GN=LOC103499555 PE=4 SV=1	[more]
A0A0A0KM05	5.2e-44	96.59	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G523480 PE=4 SV=1	[more]
A0A6J1J637	9.2e-41	92.05	probable helicase MAGATAMA 3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482...	[more]
A0A6J1J2J7	9.2e-41	92.05	probable helicase MAGATAMA 3 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111482...	[more]

BLAST of Chy8G147730 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008460794.1	4.13e-52	97.73	PREDICTED: probable helicase MAGATAMA 3 [Cucumis melo] >KAA0053183.1 putative he...	[more]
XP_011658296.1	5.70e-52	96.59	probable helicase MAGATAMA 3 [Cucumis sativus] >KGN49407.1 hypothetical protein ...	[more]
XP_022935507.1	2.91e-48	92.05	probable helicase MAGATAMA 3 isoform X2 [Cucurbita moschata]	[more]
XP_023528759.1	2.93e-48	92.05	probable helicase MAGATAMA 3 isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_022983526.1	3.42e-48	92.05	probable helicase MAGATAMA 3 isoform X2 [Cucurbita maxima]	[more]

BLAST of Chy8G147730 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G15570.1	1.8e-28	61.80	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]
AT2G19120.1	6.4e-10	36.96	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]
AT4G30100.1	4.1e-09	35.87	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR041679	DNA2/NAM7 helicase-like, C-terminal	PFAM	PF13087	AAA_12	coord: 3..91 e-value: 1.9E-15 score: 57.0
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 1..91 e-value: 1.3E-12 score: 49.4
None	No IPR available	PANTHER	PTHR10887:SF489	BNAC01G22370D PROTEIN	coord: 3..91
IPR045055	DNA2/NAM7-like helicase	PANTHER	PTHR10887	DNA2/NAM7 HELICASE FAMILY	coord: 3..91

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy8G147730.1	Chy8G147730.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009451	RNA modification
cellular_component	GO:0043231	intracellular membrane-bounded organelle
molecular_function	GO:0004386	helicase activity
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0003723	RNA binding