Chy8G147140 (gene) Cucumber (hystrix) v1

Overview
NameChy8G147140
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptiontriosephosphate isomerase, cytosolic
LocationchrH08: 2454680 .. 2457972 (+)
RNA-Seq ExpressionChy8G147140
SyntenyChy8G147140
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAAGAAAATTTTTCGTCGGCGGCAACTGGAAATGCGTATACTTCTTACTTCTTATTTCAAATTATTTTCCCTTTTTTTTTTCAATCTTTATTTTCTCTCCATCTTTTCGATGTTCGGCTTTTCATTCGATTCGATCCTTGTCGTTCGTGCTTTAAGATGCTAAATCTGTTGAACCTATGTGATTTCTACCTATTTGTTGAAATGTTATTGGGTTTTGATCACGAACTGAGGAACTTCCAGTTTTTCGGTGTTTTGACTTTTCAGATCTTGTGACTTTTCCGATCTGTGTGTGTTTTATGTGATTTTGGATTGTATTTGAGGTGTTCAAGTGTTAAAAATATGTGAAACTTTGGAAATGGAGGTTTGGGCGTTCTGGATTACTCTTGATTGAACTAAAACTAATCATCGTATTCATCGAATGAATATTGTACAGAATGGAACAAACGAGGAGGTGAAGAAAATTGTCAGCACGTTGAATGAAGCTGAAATTCCTTCAGAAGATGCTGTGGGTATGTATGGAAATCCCGTTTGGTTTTCAGCTGTATTAATGTTCTTATGGCTTACATTATCTGGCAGTTGTTGAAGTGATTGATTGGGTTTTACATGTTACTTTCCTTTGCAGAAGTTGTTGTGAGTCCACCTTTTGTGTTTCTTTCGCTGGTGAAGAGTTTGTTGAGGTCTGATATTCAAGTTGCAGCACAGAACTGTTGGGTTCGCAAAGGTGGTGCGTTTACTGGAGAAGTTAGGTATGATTTTTAGTTAGGGAGGTTTCTAATGGCTTGGCTACTTGATGATATCTGTGTTAATGATGAAATTTGATGTTGAATCGATAAGCCTCCGATTGGTAACATATTTCTGAAGTAAAATAGCTGTTTCTTTGCGAACTTCAAAAAACATGTTTTTAAATACTACTTGTTTTAGTTTTTAAAACATTGACTTGGTCTTTTAAAACATTGGTAAAAAGTAAATAGCAAAGCAGGAAATTTAGATTTCTTTAGAGGTGGAAATGGTTTTGATAGGCTTAATTTTATAAAGTTTAAAACTATAAATCAAATGATTACTAAAACAGGGCTAAATGTTGTCTAACTTCAGCTTTGCTCGCGTTTTCTAGGCCTTTTTCTTTTTTATATGTAAAAAATATTTCTCTTAAACTCTCAAGAATTAAGGGTGCATCGGCAATTGATTGAAGTTTATATCGTTTTGTCCTGTAGTGCTGAGATGCTTGTCAATTTAGGAATTCCTTGGGTTATTCTTGGGCATTCTGAAAGAAGACTTATTTTGAAAGAGTCCAATGAGGTAACCCCCTGCTAGAACAAAAACTAGTGGCCACACCCCCATCTTTATATTGTTAACTTGTCAAGGAAAAATAATTATTCACCGTCAACAAATATCACTGATGCAAAGTCCATTTCCTCATCCTGTTTATATACAGTTCGTAGGAGATAAAGTTGCATATGCACTTTCTCAAGGCATTAAAGTCATTGCTTGTGTTGGAGAAACTCTTGAGCAGCGTGAATCAGGATCAACAATTGAAGTTGTTGCTGCACAAACTAAAGCAATTGCAGGTACTTCTAAGTTTTACCTTCTTAGCTCTTACATTTAAAGTAAATGTTTGGAACGGGCAGGATCTTTGAGCTCTTGATCTTTTGCTCTTCTCATGCTGAGAAGCTTTCAATATTATACTCCCATGTTTTATATCAACATGGATAACCCTTTCTGTTACATGGCAGATAAAATCTCTAATTGGGACAATGTTGTCTTGGCTTATGAGCCAGTATGGGCTATTGGAACAGGGAAAGTTGCAACCCCTGCTCAGGCTCAAGAAGTAAGATTCGTTATTATATATTTTTGCATTTCGCATGAATTCAGGATAATGGACAATAACATTTTGTGGTTTTTTCGAAAGGTCCATGCTGAATTGAGGAAATGGTTTCAGAACAATGTTAGTGCTGAAGTTGCTGGATCTGTTAGAATTATATATGGAGGTAACCTCCTTGCTTTTATGAGAAAAGATTGACATGTTTGTTGTGCATTCTTTTCCTATATTTTGTCGAGGTGGTGAAAACTGTAAATCCAAGCATATTGATGAGGAAGCCTTTGTAATGGGGAGTGCGAACCCACATAAGCATGATGTAACCAAAGACCATTCGTGGGAGGGGTGTACTTTTTAGGAAACAACCAAATACAAACCTCAATTTGATTAATATGTATGCAATGTGTTAAAAAGCTTACCCAGTTCTTTTGAATTAAGCTGGTCTAAGTCCATGCCGTAGAACTTTTTCTTTATTCATTGGGTCCAGCTTCTATCTCACTCACCACAAGAACTTTGAAGACCACTGTATGCCTTCTGCATGACAATACACAAGCATTATGTATTAAATTAGGCGCACGTTGTCACAATCGCAACCTTTCAACCTCGGTCGATTGTGCGGCACTTGTTTGACCCGTCAACAAATCAGTCGATCGAAATATGAAAACCTTAACAAACTCACGGTACGATTCACCCTCCTGTCACGTTCTTGATGCGTTCTTGAGAAAATGTTTTAGAGAACGTGAGAGAGAGAAATTTGCTTGCAAAGAGTGAAGTTTGTCGGTTGCAAGGGAAAAAGTTTGCTTGCAAAGAGTGCAAGCATTACAATGCTTGGGCGGGGACTCAACTACTATGTCTCCCTTATAGTACATTTTGAACATTTCTAGCTCTGGTAGGCTTGCATATGAAAATGCCGAAACGTTACACCCAAGCTTTATGACTTCAAAAGGAAAACAACTAGCTAAACTAAATACAAGACATAAAGATAAGATAAATTGAGGGTATTTGGGCATACGGCCATAGACAAACAACGCCTTGGACAAGTATGATCGTGACACACGTCAGGGGCACATCTTATGTGATAAGTCCCCAGGCCAAAAGCTATGGGCCTTGAGACTTTTTATTTACTTTTGAAAAATAATAATAATTAGGATTTTACTTTTTATTAGCTAAAGAGAACAAAATTTATTAATACAAAATTTCTTCTTGGGGTTATTTAACTGTGGGACTGACTTGTTTAAGTTCTTAATTTGTCTTTTAGGTTCCGTAAACGGTGCAAACTGCAAAGAACTGGCAGCACAACCTGACTTGGATGGATTTTTGGTTGGAGGAGCTTCGCTTAAGGTTAACTCTTCTTATAAGTTTGCATCGTTCATTTTTGTTAATTTGTTCTGTTGTTGACTTGTCTCTATTTTTTCTACAAACTAGCCTGAGTTTGTTGACATTATTAAGTCTGCTATGGTGAAGAAATCTTCATGA

mRNA sequence

ATGGCAAGAAAATTTTTCGTCGGCGGCAACTGGAAATGCAATGGAACAAACGAGGAGGTGAAGAAAATTGTCAGCACGTTGAATGAAGCTGAAATTCCTTCAGAAGATGCTGTGGAAGTTGTTGTGAGTCCACCTTTTGTGTTTCTTTCGCTGGTGAAGAGTTTGTTGAGGTCTGATATTCAAGTTGCAGCACAGAACTGTTGGGTTCGCAAAGGTGGTGCGTTTACTGGAGAAGTTAGTGCTGAGATGCTTGTCAATTTAGGAATTCCTTGGGTTATTCTTGGGCATTCTGAAAGAAGACTTATTTTGAAAGAGTCCAATGAGTTCGTAGGAGATAAAGTTGCATATGCACTTTCTCAAGGCATTAAAGTCATTGCTTGTGTTGGAGAAACTCTTGAGCAGCGTGAATCAGGATCAACAATTGAAGTTGTTGCTGCACAAACTAAAGCAATTGCAGATAAAATCTCTAATTGGGACAATGTTGTCTTGGCTTATGAGCCAGTATGGGCTATTGGAACAGGGAAAGTTGCAACCCCTGCTCAGGCTCAAGAAGTCCATGCTGAATTGAGGAAATGGTTTCAGAACAATGTTAGTGCTGAAGTTGCTGGATCTGTTAGAATTATATATGGAGGTTCCGTAAACGGTGCAAACTGCAAAGAACTGGCAGCACAACCTGACTTGGATGGATTTTTGGTTGGAGGAGCTTCGCTTAAGCCTGAGTTTGTTGACATTATTAAGTCTGCTATGGTGAAGAAATCTTCATGA

Coding sequence (CDS)

ATGGCAAGAAAATTTTTCGTCGGCGGCAACTGGAAATGCAATGGAACAAACGAGGAGGTGAAGAAAATTGTCAGCACGTTGAATGAAGCTGAAATTCCTTCAGAAGATGCTGTGGAAGTTGTTGTGAGTCCACCTTTTGTGTTTCTTTCGCTGGTGAAGAGTTTGTTGAGGTCTGATATTCAAGTTGCAGCACAGAACTGTTGGGTTCGCAAAGGTGGTGCGTTTACTGGAGAAGTTAGTGCTGAGATGCTTGTCAATTTAGGAATTCCTTGGGTTATTCTTGGGCATTCTGAAAGAAGACTTATTTTGAAAGAGTCCAATGAGTTCGTAGGAGATAAAGTTGCATATGCACTTTCTCAAGGCATTAAAGTCATTGCTTGTGTTGGAGAAACTCTTGAGCAGCGTGAATCAGGATCAACAATTGAAGTTGTTGCTGCACAAACTAAAGCAATTGCAGATAAAATCTCTAATTGGGACAATGTTGTCTTGGCTTATGAGCCAGTATGGGCTATTGGAACAGGGAAAGTTGCAACCCCTGCTCAGGCTCAAGAAGTCCATGCTGAATTGAGGAAATGGTTTCAGAACAATGTTAGTGCTGAAGTTGCTGGATCTGTTAGAATTATATATGGAGGTTCCGTAAACGGTGCAAACTGCAAAGAACTGGCAGCACAACCTGACTTGGATGGATTTTTGGTTGGAGGAGCTTCGCTTAAGCCTGAGTTTGTTGACATTATTAAGTCTGCTATGGTGAAGAAATCTTCATGA

Protein sequence

MARKFFVGGNWKCNGTNEEVKKIVSTLNEAEIPSEDAVEVVVSPPFVFLSLVKSLLRSDIQVAAQNCWVRKGGAFTGEVSAEMLVNLGIPWVILGHSERRLILKESNEFVGDKVAYALSQGIKVIACVGETLEQRESGSTIEVVAAQTKAIADKISNWDNVVLAYEPVWAIGTGKVATPAQAQEVHAELRKWFQNNVSAEVAGSVRIIYGGSVNGANCKELAAQPDLDGFLVGGASLKPEFVDIIKSAMVKKSS*
Homology
BLAST of Chy8G147140 vs. ExPASy Swiss-Prot
Match: P21820 (Triosephosphate isomerase, cytosolic OS=Coptis japonica OX=3442 PE=2 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 5.8e-122
Identity = 214/253 (84.58%), Postives = 233/253 (92.09%), Query Frame = 0

Query: 1   MARKFFVGGNWKCNGTNEEVKKIVSTLNEAEIPSEDAVEVVVSPPFVFLSLVKSLLRSDI 60
           M RKFFVGGNWKCNGT+EEVKKIV+ LNEAE+PSED VEVVVSPP+VFL  VK+LLR+D 
Sbjct: 1   MGRKFFVGGNWKCNGTSEEVKKIVTLLNEAEVPSEDVVEVVVSPPYVFLPFVKNLLRADF 60

Query: 61  QVAAQNCWVRKGGAFTGEVSAEMLVNLGIPWVILGHSERRLILKESNEFVGDKVAYALSQ 120
            VAAQNCWV+KGGAFTGEVSAEMLVNLGIPWVILGHSERR +L ESNEFVGDK AYALSQ
Sbjct: 61  HVAAQNCWVKKGGAFTGEVSAEMLVNLGIPWVILGHSERRALLNESNEFVGDKTAYALSQ 120

Query: 121 GIKVIACVGETLEQRESGSTIEVVAAQTKAIADKISNWDNVVLAYEPVWAIGTGKVATPA 180
           G+KVIACVGETLEQRE+GSTI VVAAQTKAIA+K+S+W N+V+AYEPVWAIGTGKVA+PA
Sbjct: 121 GLKVIACVGETLEQREAGSTISVVAAQTKAIAEKVSDWTNIVVAYEPVWAIGTGKVASPA 180

Query: 181 QAQEVHAELRKWFQNNVSAEVAGSVRIIYGGSVNGANCKELAAQPDLDGFLVGGASLKPE 240
           QAQEVH ELRKW + NV A+VAGSVRIIYGGSVNGAN KELA QPD+DGFLVGGASLKPE
Sbjct: 181 QAQEVHFELRKWIKENVGADVAGSVRIIYGGSVNGANSKELAGQPDIDGFLVGGASLKPE 240

Query: 241 FVDIIKSAMVKKS 254
           FVDIIKSA VK S
Sbjct: 241 FVDIIKSATVKSS 253

BLAST of Chy8G147140 vs. ExPASy Swiss-Prot
Match: P48491 (Triosephosphate isomerase, cytosolic OS=Arabidopsis thaliana OX=3702 GN=CTIMC PE=1 SV=2)

HSP 1 Score: 430.3 bits (1105), Expect = 1.6e-119
Identity = 209/254 (82.28%), Postives = 233/254 (91.73%), Query Frame = 0

Query: 1   MARKFFVGGNWKCNGTNEEVKKIVSTLNEAEIPSEDAVEVVVSPPFVFLSLVKSLLRSDI 60
           MARKFFVGGNWKCNGT EEVKKIV+TLNEA++PS+D VEVVVSPP+VFL LVKS LRSD 
Sbjct: 1   MARKFFVGGNWKCNGTAEEVKKIVNTLNEAQVPSQDVVEVVVSPPYVFLPLVKSTLRSDF 60

Query: 61  QVAAQNCWVRKGGAFTGEVSAEMLVNLGIPWVILGHSERRLILKESNEFVGDKVAYALSQ 120
            VAAQNCWV+KGGAFTGEVSAEMLVNL IPWVILGHSERR IL ES+EFVGDKVAYAL+Q
Sbjct: 61  FVAAQNCWVKKGGAFTGEVSAEMLVNLDIPWVILGHSERRAILNESSEFVGDKVAYALAQ 120

Query: 121 GIKVIACVGETLEQRESGSTIEVVAAQTKAIADKISNWDNVVLAYEPVWAIGTGKVATPA 180
           G+KVIACVGETLE+RE+GST++VVAAQTKAIAD+++NW NVV+AYEPVWAIGTGKVA+PA
Sbjct: 121 GLKVIACVGETLEEREAGSTMDVVAAQTKAIADRVTNWSNVVIAYEPVWAIGTGKVASPA 180

Query: 181 QAQEVHAELRKWFQNNVSAEVAGSVRIIYGGSVNGANCKELAAQPDLDGFLVGGASLKPE 240
           QAQEVH ELRKW   NVSA+VA + RIIYGGSVNG NCKEL  Q D+DGFLVGGASLKPE
Sbjct: 181 QAQEVHDELRKWLAKNVSADVAATTRIIYGGSVNGGNCKELGGQADVDGFLVGGASLKPE 240

Query: 241 FVDIIKSAMVKKSS 255
           F+DIIK+A VKKS+
Sbjct: 241 FIDIIKAAEVKKSA 254

BLAST of Chy8G147140 vs. ExPASy Swiss-Prot
Match: P48495 (Triosephosphate isomerase, cytosolic OS=Petunia hybrida OX=4102 GN=TPIP1 PE=2 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 1.7e-118
Identity = 207/254 (81.50%), Postives = 231/254 (90.94%), Query Frame = 0

Query: 1   MARKFFVGGNWKCNGTNEEVKKIVSTLNEAEIPSEDAVEVVVSPPFVFLSLVKSLLRSDI 60
           M RKFFVGGNWKCNGT EEVKKI++TLN A++PS+D VEVVVSPP+VFL LVK+ LR D 
Sbjct: 1   MGRKFFVGGNWKCNGTAEEVKKILATLNAADVPSQDVVEVVVSPPYVFLPLVKNELRPDF 60

Query: 61  QVAAQNCWVRKGGAFTGEVSAEMLVNLGIPWVILGHSERRLILKESNEFVGDKVAYALSQ 120
            VAAQNCWV+KGGAFTGEVSAEMLVNL IPWVILGHSERR +L ESNEFVGDKVAYALSQ
Sbjct: 61  HVAAQNCWVKKGGAFTGEVSAEMLVNLSIPWVILGHSERRALLGESNEFVGDKVAYALSQ 120

Query: 121 GIKVIACVGETLEQRESGSTIEVVAAQTKAIADKISNWDNVVLAYEPVWAIGTGKVATPA 180
           G+KVIACVGETLE+RESGST++VVAAQTKAIAD++ +W NVV+AYEPVWAIGTGKVA+PA
Sbjct: 121 GLKVIACVGETLEERESGSTMDVVAAQTKAIADRVKDWTNVVVAYEPVWAIGTGKVASPA 180

Query: 181 QAQEVHAELRKWFQNNVSAEVAGSVRIIYGGSVNGANCKELAAQPDLDGFLVGGASLKPE 240
           QAQEVHAELRKW   NVS EVA S RIIYGGSVNGANCKEL  QPD+DGFLVGGASLKPE
Sbjct: 181 QAQEVHAELRKWLAANVSPEVAASTRIIYGGSVNGANCKELGGQPDVDGFLVGGASLKPE 240

Query: 241 FVDIIKSAMVKKSS 255
           F+DIIK+A VK+++
Sbjct: 241 FIDIIKAAEVKRNA 254

BLAST of Chy8G147140 vs. ExPASy Swiss-Prot
Match: P12863 (Triosephosphate isomerase, cytosolic OS=Zea mays OX=4577 PE=1 SV=3)

HSP 1 Score: 416.4 bits (1069), Expect = 2.3e-115
Identity = 201/253 (79.45%), Postives = 224/253 (88.54%), Query Frame = 0

Query: 1   MARKFFVGGNWKCNGTNEEVKKIVSTLNEAEIPSEDAVEVVVSPPFVFLSLVKSLLRSDI 60
           M RKFFVGGNWKCNGT ++V+KIV TLNE ++P  D VEVVVSPP+VFL +VKS LR + 
Sbjct: 1   MGRKFFVGGNWKCNGTTDQVEKIVKTLNEGQVPPSDVVEVVVSPPYVFLPVVKSQLRQEF 60

Query: 61  QVAAQNCWVRKGGAFTGEVSAEMLVNLGIPWVILGHSERRLILKESNEFVGDKVAYALSQ 120
            VAAQNCWV+KGGAFTGEVSAEMLVNLG+PWVILGHSERR +L ESNEFVGDKVAYALSQ
Sbjct: 61  HVAAQNCWVKKGGAFTGEVSAEMLVNLGVPWVILGHSERRALLGESNEFVGDKVAYALSQ 120

Query: 121 GIKVIACVGETLEQRESGSTIEVVAAQTKAIADKISNWDNVVLAYEPVWAIGTGKVATPA 180
           G+KVIACVGETLEQRE+GST++VVAAQTKAIA+KI +W NVV+AYEPVWAIGTGKVATPA
Sbjct: 121 GLKVIACVGETLEQREAGSTMDVVAAQTKAIAEKIKDWSNVVVAYEPVWAIGTGKVATPA 180

Query: 181 QAQEVHAELRKWFQNNVSAEVAGSVRIIYGGSVNGANCKELAAQPDLDGFLVGGASLKPE 240
           QAQEVHA LR W + N S EVA S RIIYGGSV  ANCKELAAQPD+DGFLVGGASLKPE
Sbjct: 181 QAQEVHASLRDWLKTNASPEVAESTRIIYGGSVTAANCKELAAQPDVDGFLVGGASLKPE 240

Query: 241 FVDIIKSAMVKKS 254
           F+DII +A VK +
Sbjct: 241 FIDIINAATVKSA 253

BLAST of Chy8G147140 vs. ExPASy Swiss-Prot
Match: P48494 (Triosephosphate isomerase, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=TPI PE=1 SV=3)

HSP 1 Score: 412.1 bits (1058), Expect = 4.4e-114
Identity = 204/253 (80.63%), Postives = 224/253 (88.54%), Query Frame = 0

Query: 1   MARKFFVGGNWKCNGTNEEVKKIVSTLNEAEIPSEDAVEVVVSPPFVFLSLVKSLLRSDI 60
           M RKFFVGGNWKCNGT ++V KIV  LNE +I S D VEVVVSPP+VFL +VKS LR +I
Sbjct: 1   MGRKFFVGGNWKCNGTTDQVDKIVKILNEGQIASTDVVEVVVSPPYVFLPVVKSQLRPEI 60

Query: 61  QVAAQNCWVRKGGAFTGEVSAEMLVNLGIPWVILGHSERRLILKESNEFVGDKVAYALSQ 120
           QVAAQNCWV+KGGAFTGEVSAEMLVNL IPWVILGHSERR +L ESNEFVGDKVAYALSQ
Sbjct: 61  QVAAQNCWVKKGGAFTGEVSAEMLVNLSIPWVILGHSERRSLLGESNEFVGDKVAYALSQ 120

Query: 121 GIKVIACVGETLEQRESGSTIEVVAAQTKAIADKISNWDNVVLAYEPVWAIGTGKVATPA 180
           G+KVIACVGETLEQRESGST++VVAAQTKAI+++I +W NVV+AYEPVWAIGTGKVATP 
Sbjct: 121 GLKVIACVGETLEQRESGSTMDVVAAQTKAISERIKDWTNVVVAYEPVWAIGTGKVATPD 180

Query: 181 QAQEVHAELRKWFQNNVSAEVAGSVRIIYGGSVNGANCKELAAQPDLDGFLVGGASLKPE 240
           QAQEVH  LRKW   NVSAEVA S RIIYGGSV GANCKELAA+PD+DGFLVGGASLKPE
Sbjct: 181 QAQEVHDGLRKWLAANVSAEVAESTRIIYGGSVTGANCKELAAKPDVDGFLVGGASLKPE 240

Query: 241 FVDIIKSAMVKKS 254
           F+DII SA VK +
Sbjct: 241 FIDIINSATVKSA 253

BLAST of Chy8G147140 vs. ExPASy TrEMBL
Match: A0A1S3B4U7 (triosephosphate isomerase, cytosolic OS=Cucumis melo OX=3656 GN=LOC103486035 PE=3 SV=1)

HSP 1 Score: 495.0 bits (1273), Expect = 1.9e-136
Identity = 251/254 (98.82%), Postives = 251/254 (98.82%), Query Frame = 0

Query: 1   MARKFFVGGNWKCNGTNEEVKKIVSTLNEAEIPSEDAVEVVVSPPFVFLSLVKSLLRSDI 60
           MARKFFVGGNWKCNGTNEEVKKIVSTLNEAEIPSEDAVEVVVSPPFVFLSLVKSLLRSDI
Sbjct: 1   MARKFFVGGNWKCNGTNEEVKKIVSTLNEAEIPSEDAVEVVVSPPFVFLSLVKSLLRSDI 60

Query: 61  QVAAQNCWVRKGGAFTGEVSAEMLVNLGIPWVILGHSERRLILKESNEFVGDKVAYALSQ 120
           QVAAQNCWVRKGGAFTGEVSAEMLVNLGIPWVILGHSERRLIL ESNEFVGDKVAYALSQ
Sbjct: 61  QVAAQNCWVRKGGAFTGEVSAEMLVNLGIPWVILGHSERRLILNESNEFVGDKVAYALSQ 120

Query: 121 GIKVIACVGETLEQRESGSTIEVVAAQTKAIADKISNWDNVVLAYEPVWAIGTGKVATPA 180
           GIKVIACVGETLEQRESGSTIEVVAAQTKAIADKISNWDNVVLAYEPVWAIGTGKVATPA
Sbjct: 121 GIKVIACVGETLEQRESGSTIEVVAAQTKAIADKISNWDNVVLAYEPVWAIGTGKVATPA 180

Query: 181 QAQEVHAELRKWFQNNVSAEVAGSVRIIYGGSVNGANCKELAAQPDLDGFLVGGASLKPE 240
           QAQEVHAELRKWFQ NVSAEVAGSVRIIYGGSVNGANCKELAAQPDLDGFLVGGASLKPE
Sbjct: 181 QAQEVHAELRKWFQTNVSAEVAGSVRIIYGGSVNGANCKELAAQPDLDGFLVGGASLKPE 240

Query: 241 FVDIIKSAMVKKSS 255
           FVDIIKSA VKKSS
Sbjct: 241 FVDIIKSATVKKSS 254

BLAST of Chy8G147140 vs. ExPASy TrEMBL
Match: A0A0A0KZL7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G598000 PE=3 SV=1)

HSP 1 Score: 494.2 bits (1271), Expect = 3.3e-136
Identity = 250/254 (98.43%), Postives = 252/254 (99.21%), Query Frame = 0

Query: 1   MARKFFVGGNWKCNGTNEEVKKIVSTLNEAEIPSEDAVEVVVSPPFVFLSLVKSLLRSDI 60
           MARKFFVGGNWKCNGTNEEVKKIVSTLNEAEIPSEDAVEVVVSPPFVFLSLVKSLLRSDI
Sbjct: 1   MARKFFVGGNWKCNGTNEEVKKIVSTLNEAEIPSEDAVEVVVSPPFVFLSLVKSLLRSDI 60

Query: 61  QVAAQNCWVRKGGAFTGEVSAEMLVNLGIPWVILGHSERRLILKESNEFVGDKVAYALSQ 120
           QVAAQNCWVRKGGAFTGEVSAEMLVNLGIPWVILGHSERRLILKESNEFVGDKVAYALSQ
Sbjct: 61  QVAAQNCWVRKGGAFTGEVSAEMLVNLGIPWVILGHSERRLILKESNEFVGDKVAYALSQ 120

Query: 121 GIKVIACVGETLEQRESGSTIEVVAAQTKAIADKISNWDNVVLAYEPVWAIGTGKVATPA 180
           GIKVIACVGETLEQRESGSTIEVVAAQTKAIA+KISNWDNVVLAYEPVWAIGTGKVATPA
Sbjct: 121 GIKVIACVGETLEQRESGSTIEVVAAQTKAIAEKISNWDNVVLAYEPVWAIGTGKVATPA 180

Query: 181 QAQEVHAELRKWFQNNVSAEVAGSVRIIYGGSVNGANCKELAAQPDLDGFLVGGASLKPE 240
           QAQEVHAELRKWFQNNVSAEVAGSVRIIYGGSVNGANCKELAAQPDLDGFLVGGASLK E
Sbjct: 181 QAQEVHAELRKWFQNNVSAEVAGSVRIIYGGSVNGANCKELAAQPDLDGFLVGGASLKAE 240

Query: 241 FVDIIKSAMVKKSS 255
           F+DIIKSA VKKSS
Sbjct: 241 FIDIIKSATVKKSS 254

BLAST of Chy8G147140 vs. ExPASy TrEMBL
Match: A0A6J1FY28 (triosephosphate isomerase, cytosolic-like OS=Cucurbita moschata OX=3662 GN=LOC111448904 PE=3 SV=1)

HSP 1 Score: 474.6 bits (1220), Expect = 2.7e-130
Identity = 235/254 (92.52%), Postives = 246/254 (96.85%), Query Frame = 0

Query: 1   MARKFFVGGNWKCNGTNEEVKKIVSTLNEAEIPSEDAVEVVVSPPFVFLSLVKSLLRSDI 60
           MARKFFVGGNWKCNGTNEE KKIVSTLN+AEIPSED VEVVVSPPFVFLSLVKSLLRSDI
Sbjct: 1   MARKFFVGGNWKCNGTNEETKKIVSTLNQAEIPSEDVVEVVVSPPFVFLSLVKSLLRSDI 60

Query: 61  QVAAQNCWVRKGGAFTGEVSAEMLVNLGIPWVILGHSERRLILKESNEFVGDKVAYALSQ 120
           QVAAQNCWVRKGGAFTGE+SAEMLVNLGIPWVI+GHSERRL+L ESNEFVGDKVAYALSQ
Sbjct: 61  QVAAQNCWVRKGGAFTGEISAEMLVNLGIPWVIIGHSERRLLLNESNEFVGDKVAYALSQ 120

Query: 121 GIKVIACVGETLEQRESGSTIEVVAAQTKAIADKISNWDNVVLAYEPVWAIGTGKVATPA 180
           GIKVIACVGETLEQRESGST++VVAAQTKAIADKIS+WDNVVLAYEPVWAIGTGKVATPA
Sbjct: 121 GIKVIACVGETLEQRESGSTLDVVAAQTKAIADKISHWDNVVLAYEPVWAIGTGKVATPA 180

Query: 181 QAQEVHAELRKWFQNNVSAEVAGSVRIIYGGSVNGANCKELAAQPDLDGFLVGGASLKPE 240
           QAQEVH+ELRKWF NNVSAEV  S+RIIYGGSVNGANCKELAAQPDLDGFLVGGAS+KPE
Sbjct: 181 QAQEVHSELRKWFHNNVSAEVPESIRIIYGGSVNGANCKELAAQPDLDGFLVGGASVKPE 240

Query: 241 FVDIIKSAMVKKSS 255
           FVDII SA VKKS+
Sbjct: 241 FVDIINSATVKKSA 254

BLAST of Chy8G147140 vs. ExPASy TrEMBL
Match: A0A6J1JHF9 (triosephosphate isomerase, cytosolic-like OS=Cucurbita maxima OX=3661 GN=LOC111484521 PE=3 SV=1)

HSP 1 Score: 473.8 bits (1218), Expect = 4.6e-130
Identity = 234/254 (92.13%), Postives = 246/254 (96.85%), Query Frame = 0

Query: 1   MARKFFVGGNWKCNGTNEEVKKIVSTLNEAEIPSEDAVEVVVSPPFVFLSLVKSLLRSDI 60
           MARKFF+GGNWKCNGTNEE KKIVSTLN+AEIPSED VEVVVSPPFVFLSLVKSLLRSDI
Sbjct: 1   MARKFFIGGNWKCNGTNEETKKIVSTLNQAEIPSEDVVEVVVSPPFVFLSLVKSLLRSDI 60

Query: 61  QVAAQNCWVRKGGAFTGEVSAEMLVNLGIPWVILGHSERRLILKESNEFVGDKVAYALSQ 120
           QVAAQNCWVRKGGAFTGE+SAEMLVNLGIPWVI+GHSERRL+L ESNEFVGDKVAYALSQ
Sbjct: 61  QVAAQNCWVRKGGAFTGEISAEMLVNLGIPWVIIGHSERRLLLNESNEFVGDKVAYALSQ 120

Query: 121 GIKVIACVGETLEQRESGSTIEVVAAQTKAIADKISNWDNVVLAYEPVWAIGTGKVATPA 180
           GIKVIACVGETLEQRESGST++VVAAQTKAIADKIS+WDNVVLAYEPVWAIGTGKVATPA
Sbjct: 121 GIKVIACVGETLEQRESGSTLDVVAAQTKAIADKISHWDNVVLAYEPVWAIGTGKVATPA 180

Query: 181 QAQEVHAELRKWFQNNVSAEVAGSVRIIYGGSVNGANCKELAAQPDLDGFLVGGASLKPE 240
           QAQEVH+ELRKWF  NVSAEVA S+RIIYGGSVNGANCKELAAQPDLDGFLVGGAS+KPE
Sbjct: 181 QAQEVHSELRKWFHKNVSAEVAESIRIIYGGSVNGANCKELAAQPDLDGFLVGGASVKPE 240

Query: 241 FVDIIKSAMVKKSS 255
           FVDII SA VKKS+
Sbjct: 241 FVDIINSATVKKSA 254

BLAST of Chy8G147140 vs. ExPASy TrEMBL
Match: A0A6J1L7L1 (triosephosphate isomerase, cytosolic OS=Cucurbita maxima OX=3661 GN=LOC111499917 PE=3 SV=1)

HSP 1 Score: 470.3 bits (1209), Expect = 5.1e-129
Identity = 234/252 (92.86%), Postives = 244/252 (96.83%), Query Frame = 0

Query: 1   MARKFFVGGNWKCNGTNEEVKKIVSTLNEAEIPSEDAVEVVVSPPFVFLSLVKSLLRSDI 60
           MARKFFVGGNWKCNGT EEVKKIV TLNEAEIPSED VEVVVSPPFVFLSLVK+LLRS+I
Sbjct: 1   MARKFFVGGNWKCNGTTEEVKKIVGTLNEAEIPSEDVVEVVVSPPFVFLSLVKNLLRSEI 60

Query: 61  QVAAQNCWVRKGGAFTGEVSAEMLVNLGIPWVILGHSERRLILKESNEFVGDKVAYALSQ 120
           QVAAQNCWVRKGGAFTGEVSAEMLVNL IPWVI+GHSERRLIL ESNEFVGDKVAYALSQ
Sbjct: 61  QVAAQNCWVRKGGAFTGEVSAEMLVNLAIPWVIIGHSERRLILNESNEFVGDKVAYALSQ 120

Query: 121 GIKVIACVGETLEQRESGSTIEVVAAQTKAIADKISNWDNVVLAYEPVWAIGTGKVATPA 180
           GIKVIACVGETLEQRESG+T+EVVAAQTKAIA+KISNWDNVVLAYEPVWAIGTGKVA+PA
Sbjct: 121 GIKVIACVGETLEQRESGATLEVVAAQTKAIAEKISNWDNVVLAYEPVWAIGTGKVASPA 180

Query: 181 QAQEVHAELRKWFQNNVSAEVAGSVRIIYGGSVNGANCKELAAQPDLDGFLVGGASLKPE 240
           QAQEVH ELRKWF +NVSA+VAGS+RIIYGGSVNGANCKELAAQPDLDGFLVGGASLKPE
Sbjct: 181 QAQEVHHELRKWFSDNVSADVAGSIRIIYGGSVNGANCKELAAQPDLDGFLVGGASLKPE 240

Query: 241 FVDIIKSAMVKK 253
           FVDIIKSA VKK
Sbjct: 241 FVDIIKSATVKK 252

BLAST of Chy8G147140 vs. NCBI nr
Match: XP_008442057.1 (PREDICTED: triosephosphate isomerase, cytosolic [Cucumis melo])

HSP 1 Score: 492 bits (1267), Expect = 3.11e-175
Identity = 251/254 (98.82%), Postives = 251/254 (98.82%), Query Frame = 0

Query: 1   MARKFFVGGNWKCNGTNEEVKKIVSTLNEAEIPSEDAVEVVVSPPFVFLSLVKSLLRSDI 60
           MARKFFVGGNWKCNGTNEEVKKIVSTLNEAEIPSEDAVEVVVSPPFVFLSLVKSLLRSDI
Sbjct: 1   MARKFFVGGNWKCNGTNEEVKKIVSTLNEAEIPSEDAVEVVVSPPFVFLSLVKSLLRSDI 60

Query: 61  QVAAQNCWVRKGGAFTGEVSAEMLVNLGIPWVILGHSERRLILKESNEFVGDKVAYALSQ 120
           QVAAQNCWVRKGGAFTGEVSAEMLVNLGIPWVILGHSERRLIL ESNEFVGDKVAYALSQ
Sbjct: 61  QVAAQNCWVRKGGAFTGEVSAEMLVNLGIPWVILGHSERRLILNESNEFVGDKVAYALSQ 120

Query: 121 GIKVIACVGETLEQRESGSTIEVVAAQTKAIADKISNWDNVVLAYEPVWAIGTGKVATPA 180
           GIKVIACVGETLEQRESGSTIEVVAAQTKAIADKISNWDNVVLAYEPVWAIGTGKVATPA
Sbjct: 121 GIKVIACVGETLEQRESGSTIEVVAAQTKAIADKISNWDNVVLAYEPVWAIGTGKVATPA 180

Query: 181 QAQEVHAELRKWFQNNVSAEVAGSVRIIYGGSVNGANCKELAAQPDLDGFLVGGASLKPE 240
           QAQEVHAELRKWFQ NVSAEVAGSVRIIYGGSVNGANCKELAAQPDLDGFLVGGASLKPE
Sbjct: 181 QAQEVHAELRKWFQTNVSAEVAGSVRIIYGGSVNGANCKELAAQPDLDGFLVGGASLKPE 240

Query: 241 FVDIIKSAMVKKSS 254
           FVDIIKSA VKKSS
Sbjct: 241 FVDIIKSATVKKSS 254

BLAST of Chy8G147140 vs. NCBI nr
Match: XP_004144643.1 (triosephosphate isomerase, cytosolic [Cucumis sativus] >KGN54918.1 hypothetical protein Csa_012408 [Cucumis sativus])

HSP 1 Score: 491 bits (1265), Expect = 6.27e-175
Identity = 250/254 (98.43%), Postives = 252/254 (99.21%), Query Frame = 0

Query: 1   MARKFFVGGNWKCNGTNEEVKKIVSTLNEAEIPSEDAVEVVVSPPFVFLSLVKSLLRSDI 60
           MARKFFVGGNWKCNGTNEEVKKIVSTLNEAEIPSEDAVEVVVSPPFVFLSLVKSLLRSDI
Sbjct: 1   MARKFFVGGNWKCNGTNEEVKKIVSTLNEAEIPSEDAVEVVVSPPFVFLSLVKSLLRSDI 60

Query: 61  QVAAQNCWVRKGGAFTGEVSAEMLVNLGIPWVILGHSERRLILKESNEFVGDKVAYALSQ 120
           QVAAQNCWVRKGGAFTGEVSAEMLVNLGIPWVILGHSERRLILKESNEFVGDKVAYALSQ
Sbjct: 61  QVAAQNCWVRKGGAFTGEVSAEMLVNLGIPWVILGHSERRLILKESNEFVGDKVAYALSQ 120

Query: 121 GIKVIACVGETLEQRESGSTIEVVAAQTKAIADKISNWDNVVLAYEPVWAIGTGKVATPA 180
           GIKVIACVGETLEQRESGSTIEVVAAQTKAIA+KISNWDNVVLAYEPVWAIGTGKVATPA
Sbjct: 121 GIKVIACVGETLEQRESGSTIEVVAAQTKAIAEKISNWDNVVLAYEPVWAIGTGKVATPA 180

Query: 181 QAQEVHAELRKWFQNNVSAEVAGSVRIIYGGSVNGANCKELAAQPDLDGFLVGGASLKPE 240
           QAQEVHAELRKWFQNNVSAEVAGSVRIIYGGSVNGANCKELAAQPDLDGFLVGGASLK E
Sbjct: 181 QAQEVHAELRKWFQNNVSAEVAGSVRIIYGGSVNGANCKELAAQPDLDGFLVGGASLKAE 240

Query: 241 FVDIIKSAMVKKSS 254
           F+DIIKSA VKKSS
Sbjct: 241 FIDIIKSATVKKSS 254

BLAST of Chy8G147140 vs. NCBI nr
Match: XP_038881012.1 (triosephosphate isomerase, cytosolic [Benincasa hispida])

HSP 1 Score: 485 bits (1248), Expect = 2.45e-172
Identity = 245/254 (96.46%), Postives = 249/254 (98.03%), Query Frame = 0

Query: 1   MARKFFVGGNWKCNGTNEEVKKIVSTLNEAEIPSEDAVEVVVSPPFVFLSLVKSLLRSDI 60
           MARKFFVGGNWKCNGT EEVKKIVSTLNEAEIPSEDAVEVVVSPPFVFLSLVKSLLRSDI
Sbjct: 1   MARKFFVGGNWKCNGTTEEVKKIVSTLNEAEIPSEDAVEVVVSPPFVFLSLVKSLLRSDI 60

Query: 61  QVAAQNCWVRKGGAFTGEVSAEMLVNLGIPWVILGHSERRLILKESNEFVGDKVAYALSQ 120
           QVAAQNCWVRKGGAFTGEVSAEMLVNLGIPWVI+GHSERRL+L ESNEFVGDKVAYALSQ
Sbjct: 61  QVAAQNCWVRKGGAFTGEVSAEMLVNLGIPWVIIGHSERRLLLNESNEFVGDKVAYALSQ 120

Query: 121 GIKVIACVGETLEQRESGSTIEVVAAQTKAIADKISNWDNVVLAYEPVWAIGTGKVATPA 180
           GIKVIACVGETLEQRESGST++VVAAQTKAIADKISNWDNVVLAYEPVWAIGTGKVATPA
Sbjct: 121 GIKVIACVGETLEQRESGSTLDVVAAQTKAIADKISNWDNVVLAYEPVWAIGTGKVATPA 180

Query: 181 QAQEVHAELRKWFQNNVSAEVAGSVRIIYGGSVNGANCKELAAQPDLDGFLVGGASLKPE 240
           QAQEVHAELRKW QNNVSAEVAGSVRIIYGGSVNGANCKELA QPDLDGFLVGGASLKPE
Sbjct: 181 QAQEVHAELRKWLQNNVSAEVAGSVRIIYGGSVNGANCKELAGQPDLDGFLVGGASLKPE 240

Query: 241 FVDIIKSAMVKKSS 254
           FVDIIKSA VKKSS
Sbjct: 241 FVDIIKSATVKKSS 254

BLAST of Chy8G147140 vs. NCBI nr
Match: KAG6570705.1 (Triosephosphate isomerase, cytosolic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7010550.1 Triosephosphate isomerase, cytosolic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 474 bits (1219), Expect = 6.45e-168
Identity = 236/254 (92.91%), Postives = 247/254 (97.24%), Query Frame = 0

Query: 1   MARKFFVGGNWKCNGTNEEVKKIVSTLNEAEIPSEDAVEVVVSPPFVFLSLVKSLLRSDI 60
           MARKFFVGGNWKCNGTNEE KKIVSTLN+AEIPSED VEVVVSPPFVFLSLVKSLLRSDI
Sbjct: 1   MARKFFVGGNWKCNGTNEETKKIVSTLNQAEIPSEDVVEVVVSPPFVFLSLVKSLLRSDI 60

Query: 61  QVAAQNCWVRKGGAFTGEVSAEMLVNLGIPWVILGHSERRLILKESNEFVGDKVAYALSQ 120
           QVAAQNCWVRKGGAFTGE+SAEMLVNLGIPWVI+GHSERRL+L ESNEFVGDKVAYALSQ
Sbjct: 61  QVAAQNCWVRKGGAFTGEISAEMLVNLGIPWVIIGHSERRLLLNESNEFVGDKVAYALSQ 120

Query: 121 GIKVIACVGETLEQRESGSTIEVVAAQTKAIADKISNWDNVVLAYEPVWAIGTGKVATPA 180
           GIKVIACVGETLEQRESGST++VVAAQTKAIADKIS+WDNVVLAYEPVWAIGTGKVATPA
Sbjct: 121 GIKVIACVGETLEQRESGSTLDVVAAQTKAIADKISHWDNVVLAYEPVWAIGTGKVATPA 180

Query: 181 QAQEVHAELRKWFQNNVSAEVAGSVRIIYGGSVNGANCKELAAQPDLDGFLVGGASLKPE 240
           QAQEVH+ELRKWF NNVSAEVA S+RIIYGGSVNGANCKELAAQPDLDGFLVGGAS+KPE
Sbjct: 181 QAQEVHSELRKWFHNNVSAEVAESIRIIYGGSVNGANCKELAAQPDLDGFLVGGASVKPE 240

Query: 241 FVDIIKSAMVKKSS 254
           FVDII SA VKKS+
Sbjct: 241 FVDIINSATVKKSA 254

BLAST of Chy8G147140 vs. NCBI nr
Match: XP_022944453.1 (triosephosphate isomerase, cytosolic-like [Cucurbita moschata])

HSP 1 Score: 472 bits (1214), Expect = 3.73e-167
Identity = 235/254 (92.52%), Postives = 246/254 (96.85%), Query Frame = 0

Query: 1   MARKFFVGGNWKCNGTNEEVKKIVSTLNEAEIPSEDAVEVVVSPPFVFLSLVKSLLRSDI 60
           MARKFFVGGNWKCNGTNEE KKIVSTLN+AEIPSED VEVVVSPPFVFLSLVKSLLRSDI
Sbjct: 1   MARKFFVGGNWKCNGTNEETKKIVSTLNQAEIPSEDVVEVVVSPPFVFLSLVKSLLRSDI 60

Query: 61  QVAAQNCWVRKGGAFTGEVSAEMLVNLGIPWVILGHSERRLILKESNEFVGDKVAYALSQ 120
           QVAAQNCWVRKGGAFTGE+SAEMLVNLGIPWVI+GHSERRL+L ESNEFVGDKVAYALSQ
Sbjct: 61  QVAAQNCWVRKGGAFTGEISAEMLVNLGIPWVIIGHSERRLLLNESNEFVGDKVAYALSQ 120

Query: 121 GIKVIACVGETLEQRESGSTIEVVAAQTKAIADKISNWDNVVLAYEPVWAIGTGKVATPA 180
           GIKVIACVGETLEQRESGST++VVAAQTKAIADKIS+WDNVVLAYEPVWAIGTGKVATPA
Sbjct: 121 GIKVIACVGETLEQRESGSTLDVVAAQTKAIADKISHWDNVVLAYEPVWAIGTGKVATPA 180

Query: 181 QAQEVHAELRKWFQNNVSAEVAGSVRIIYGGSVNGANCKELAAQPDLDGFLVGGASLKPE 240
           QAQEVH+ELRKWF NNVSAEV  S+RIIYGGSVNGANCKELAAQPDLDGFLVGGAS+KPE
Sbjct: 181 QAQEVHSELRKWFHNNVSAEVPESIRIIYGGSVNGANCKELAAQPDLDGFLVGGASVKPE 240

Query: 241 FVDIIKSAMVKKSS 254
           FVDII SA VKKS+
Sbjct: 241 FVDIINSATVKKSA 254

BLAST of Chy8G147140 vs. TAIR 10
Match: AT3G55440.1 (triosephosphate isomerase )

HSP 1 Score: 430.3 bits (1105), Expect = 1.1e-120
Identity = 209/254 (82.28%), Postives = 233/254 (91.73%), Query Frame = 0

Query: 1   MARKFFVGGNWKCNGTNEEVKKIVSTLNEAEIPSEDAVEVVVSPPFVFLSLVKSLLRSDI 60
           MARKFFVGGNWKCNGT EEVKKIV+TLNEA++PS+D VEVVVSPP+VFL LVKS LRSD 
Sbjct: 1   MARKFFVGGNWKCNGTAEEVKKIVNTLNEAQVPSQDVVEVVVSPPYVFLPLVKSTLRSDF 60

Query: 61  QVAAQNCWVRKGGAFTGEVSAEMLVNLGIPWVILGHSERRLILKESNEFVGDKVAYALSQ 120
            VAAQNCWV+KGGAFTGEVSAEMLVNL IPWVILGHSERR IL ES+EFVGDKVAYAL+Q
Sbjct: 61  FVAAQNCWVKKGGAFTGEVSAEMLVNLDIPWVILGHSERRAILNESSEFVGDKVAYALAQ 120

Query: 121 GIKVIACVGETLEQRESGSTIEVVAAQTKAIADKISNWDNVVLAYEPVWAIGTGKVATPA 180
           G+KVIACVGETLE+RE+GST++VVAAQTKAIAD+++NW NVV+AYEPVWAIGTGKVA+PA
Sbjct: 121 GLKVIACVGETLEEREAGSTMDVVAAQTKAIADRVTNWSNVVIAYEPVWAIGTGKVASPA 180

Query: 181 QAQEVHAELRKWFQNNVSAEVAGSVRIIYGGSVNGANCKELAAQPDLDGFLVGGASLKPE 240
           QAQEVH ELRKW   NVSA+VA + RIIYGGSVNG NCKEL  Q D+DGFLVGGASLKPE
Sbjct: 181 QAQEVHDELRKWLAKNVSADVAATTRIIYGGSVNGGNCKELGGQADVDGFLVGGASLKPE 240

Query: 241 FVDIIKSAMVKKSS 255
           F+DIIK+A VKKS+
Sbjct: 241 FIDIIKAAEVKKSA 254

BLAST of Chy8G147140 vs. TAIR 10
Match: AT2G21170.1 (triosephosphate isomerase )

HSP 1 Score: 319.3 bits (817), Expect = 2.8e-87
Identity = 155/250 (62.00%), Postives = 189/250 (75.60%), Query Frame = 0

Query: 4   KFFVGGNWKCNGTNEEVKKIVSTLNEAEIPSEDAVEVVVSPPFVFLSLVKSLLRSDIQVA 63
           KFFVGGNWKCNGT + + K++S LN A + ++  V+VVVSPPFV++  VKS L   I ++
Sbjct: 65  KFFVGGNWKCNGTKDSIAKLISDLNSATLEAD--VDVVVSPPFVYIDQVKSSLTDRIDIS 124

Query: 64  AQNCWVRKGGAFTGEVSAEMLVNLGIPWVILGHSERRLILKESNEFVGDKVAYALSQGIK 123
            QN WV KGGAFTGE+S E L +LG  WVILGHSERR ++ E +EF+G K AYALS+G+ 
Sbjct: 125 GQNSWVGKGGAFTGEISVEQLKDLGCKWVILGHSERRHVIGEKDEFIGKKAAYALSEGLG 184

Query: 124 VIACVGETLEQRESGSTIEVVAAQTKAIADKISNWDNVVLAYEPVWAIGTGKVATPAQAQ 183
           VIAC+GE LE+RE+G T +V  AQ KA AD + +WDN+V+AYEPVWAIGTGKVA+P QAQ
Sbjct: 185 VIACIGEKLEEREAGKTFDVCFAQLKAFADAVPSWDNIVVAYEPVWAIGTGKVASPQQAQ 244

Query: 184 EVHAELRKWFQNNVSAEVAGSVRIIYGGSVNGANCKELAAQPDLDGFLVGGASLK-PEFV 243
           EVH  +R W + NVS EVA   RIIYGGSVNG N  ELA + D+DGFLVGGASLK PEF 
Sbjct: 245 EVHVAVRGWLKKNVSEEVASKTRIIYGGSVNGGNSAELAKEEDIDGFLVGGASLKGPEFA 304

Query: 244 DIIKSAMVKK 253
            I+ S   KK
Sbjct: 305 TIVNSVTSKK 312

BLAST of Chy8G147140 vs. TAIR 10
Match: AT2G21170.2 (triosephosphate isomerase )

HSP 1 Score: 294.7 bits (753), Expect = 7.3e-80
Identity = 145/240 (60.42%), Postives = 179/240 (74.58%), Query Frame = 0

Query: 14  NGTNEEVKKIVSTLNEAEIPSEDAVEVVVSPPFVFLSLVKSLLRSDIQVAAQNCWVRKGG 73
           NGT + + K++S LN A + ++  V+VVVSPPFV++  VKS L   I ++ QN WV KGG
Sbjct: 66  NGTKDSIAKLISDLNSATLEAD--VDVVVSPPFVYIDQVKSSLTDRIDISGQNSWVGKGG 125

Query: 74  AFTGEVSAEMLVNLGIPWVILGHSERRLILKESNEFVGDKVAYALSQGIKVIACVGETLE 133
           AFTGE+S E L +LG  WVILGHSERR ++ E +EF+G K AYALS+G+ VIAC+GE LE
Sbjct: 126 AFTGEISVEQLKDLGCKWVILGHSERRHVIGEKDEFIGKKAAYALSEGLGVIACIGEKLE 185

Query: 134 QRESGSTIEVVAAQTKAIADKISNWDNVVLAYEPVWAIGTGKVATPAQAQEVHAELRKWF 193
           +RE+G T +V  AQ KA AD + +WDN+V+AYEPVWAIGTGKVA+P QAQEVH  +R W 
Sbjct: 186 EREAGKTFDVCFAQLKAFADAVPSWDNIVVAYEPVWAIGTGKVASPQQAQEVHVAVRGWL 245

Query: 194 QNNVSAEVAGSVRIIYGGSVNGANCKELAAQPDLDGFLVGGASLK-PEFVDIIKSAMVKK 253
           + NVS EVA   RIIYGGSVNG N  ELA + D+DGFLVGGASLK PEF  I+ S   KK
Sbjct: 246 KKNVSEEVASKTRIIYGGSVNGGNSAELAKEEDIDGFLVGGASLKGPEFATIVNSVTSKK 303

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P218205.8e-12284.58Triosephosphate isomerase, cytosolic OS=Coptis japonica OX=3442 PE=2 SV=1[more]
P484911.6e-11982.28Triosephosphate isomerase, cytosolic OS=Arabidopsis thaliana OX=3702 GN=CTIMC PE... [more]
P484951.7e-11881.50Triosephosphate isomerase, cytosolic OS=Petunia hybrida OX=4102 GN=TPIP1 PE=2 SV... [more]
P128632.3e-11579.45Triosephosphate isomerase, cytosolic OS=Zea mays OX=4577 PE=1 SV=3[more]
P484944.4e-11480.63Triosephosphate isomerase, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Match NameE-valueIdentityDescription
A0A1S3B4U71.9e-13698.82triosephosphate isomerase, cytosolic OS=Cucumis melo OX=3656 GN=LOC103486035 PE=... [more]
A0A0A0KZL73.3e-13698.43Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G598000 PE=3 SV=1[more]
A0A6J1FY282.7e-13092.52triosephosphate isomerase, cytosolic-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1JHF94.6e-13092.13triosephosphate isomerase, cytosolic-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1L7L15.1e-12992.86triosephosphate isomerase, cytosolic OS=Cucurbita maxima OX=3661 GN=LOC111499917... [more]
Match NameE-valueIdentityDescription
XP_008442057.13.11e-17598.82PREDICTED: triosephosphate isomerase, cytosolic [Cucumis melo][more]
XP_004144643.16.27e-17598.43triosephosphate isomerase, cytosolic [Cucumis sativus] >KGN54918.1 hypothetical ... [more]
XP_038881012.12.45e-17296.46triosephosphate isomerase, cytosolic [Benincasa hispida][more]
KAG6570705.16.45e-16892.91Triosephosphate isomerase, cytosolic, partial [Cucurbita argyrosperma subsp. sor... [more]
XP_022944453.13.73e-16792.52triosephosphate isomerase, cytosolic-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT3G55440.11.1e-12082.28triosephosphate isomerase [more]
AT2G21170.12.8e-8762.00triosephosphate isomerase [more]
AT2G21170.27.3e-8060.42triosephosphate isomerase [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000652Triosephosphate isomerasePFAMPF00121TIMcoord: 6..245
e-value: 6.8E-89
score: 297.4
IPR000652Triosephosphate isomeraseTIGRFAMTIGR00419TIGR00419coord: 6..240
e-value: 8.9E-95
score: 314.8
IPR000652Triosephosphate isomerasePANTHERPTHR21139TRIOSEPHOSPHATE ISOMERASEcoord: 1..252
IPR000652Triosephosphate isomerasePROSITEPS51440TIM_2coord: 4..247
score: 82.397606
IPR000652Triosephosphate isomeraseCDDcd00311TIMcoord: 5..246
e-value: 3.10939E-128
score: 361.083
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 1..253
e-value: 7.8E-117
score: 391.2
NoneNo IPR availablePANTHERPTHR21139:SF28TRIOSEPHOSPHATE ISOMERASEcoord: 1..252
IPR020861Triosephosphate isomerase, active sitePROSITEPS00171TIM_1coord: 164..174
IPR022896Triosephosphate isomerase, bacterial/eukaryoticHAMAPMF_00147_BTIM_Bcoord: 3..247
score: 58.422859
IPR035990Triosephosphate isomerase superfamilySUPERFAMILY51351Triosephosphate isomerase (TIM)coord: 2..246

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy8G147140.1Chy8G147140.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006094 gluconeogenesis
biological_process GO:0046166 glyceraldehyde-3-phosphate biosynthetic process
biological_process GO:0019563 glycerol catabolic process
biological_process GO:0006096 glycolytic process
cellular_component GO:0005829 cytosol
molecular_function GO:0004807 triose-phosphate isomerase activity
molecular_function GO:0003824 catalytic activity