Chy8G146190 (gene) Cucumber (hystrix) v1

Overview


Name	Chy8G146190
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Aspartic proteinase nepenthesin-1
Location	chrH08: 484359 .. 486413 (+)
RNA-Seq Expression	Chy8G146190
Synteny	Chy8G146190

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGCTGTCTCGTTACGTTCATTTGGATATCTGCAGCTTCTTTTGCTTATTGTATTCATTACAACATTATTTATTAATACATTGGCGTTCAGTTCATCTCTATCAAGGCGAGCTCTACAGAAACCAAATAAGTTGCCTAGTCATGGCTTCAAGGTGAGGCTTAAACATGTGGATCATGTGAAGAATTTGACGAGATTCGAGCGGTTGCGGCGTGGGGTGGCACGTGGGAAAAATAGATTGCATAGACTAAATGCCATGGTGTTGGCCGCTGCCAACGCTACGGTTGGTGACCAAGTGAAGGCACCGGTGGTTGCAGGTAATGGTGAGTTTCTTATGAAGTTGGCCATCGGAAGTCCGCCGAGAAGCTTTTCGGCGATCATGGATACAGGCAGCGATCTGATATGGACACAATGCAAGCCTTGTCAACAGTGTTTTGATCAAGCAACACCTATTTTTGATCCCAAACAATCTTCTTCTTTCTCTAAGATTTCATGCTCGAGCGAGCTCTGTGGCGGTCTCCCAACGTCAACATGCAGTAGCGACGGGTGCGAGTATTTGTACACATATGGAGATTCTTCCTCCACCCAAGGTGTTTTGGCTTTTGAGACCTTCACCTTTGGAGATTCAACTGAAGATCAGGTACTATTAATTTCCACTTGAAAAAAAAAAAAAAAAAAATATATATATATATATATATATATATATTTAATATTTTATATTAAAAAAATAAAAAAAAGGATTTAATATATATTGTATATATATATATATATATATATATATATATATATTATTAATTTACTCACTCATGTCTTGATATACAAGTGATTATCAATATGCTCCATCCTTACAAATAACCAAATATTAAACTAATTAATTACAGCTTTTAAGTTTGATAATTAATTCTGTTTGATGAAAATTTTGTTTTTTATTTTCTATTTGTAAACTATATTATGCACTACTTGAATTGTTTAGTCAAATTCTAAAAACTGAGGTATACTTTTAAGAACTGTTTTCTTAATTAGTTTTCAAATTTTAGTTTGTGAATAATAATAAAAAGACTAAAATCTAAATGATTATCCAACAAGATTTAAATTACAATATATTAAATTCAATTTGAATACATTTTGTTAATCCAGATATCGATCCCTGGACTCGGGTTTGGATGCGGAAACGATAACAATGGAGATGGGTTTAGCCAAGGCGCAGGGCTAGTGGGGCTTGGGCGAGGACCCTTATCATTGGTTTCTCAACTAAAAGAGCAAAAGTTTGCTTATTGTTTAACCGCCATTGATGATTCAAAACCAAGCTCTCTTTTGTTAGGATCTCTAGCAAACATAACACCTAAAACATCAAAAGATGAAATGAAAACAACCCCATTGATAAAAAACCCTTCTCAACCATCCTTTTACTATCTTTCTCTCCAAGGAATCTCAGTTGGTGGAACTCAATTATCAATACCGAAGTCCACTTTTGAGCTCCATGATGATGGGAGTGGAGGAGTAATCATAGACTCAGGCACAACAATCACTTACATTGAGAACTCAGCTTTCACTTCACTCAAAAATGAGTTCATTGCTCAAATGAATCTTCCCGTTGACGATTCCGGTACCGGTGGTCTTGACCTCTGCTTTAACCTACCTGCCGGGACAAATCAGGTAGGTCTACTTTGAAAAGTTTAACTTAATAGGTTACAGTATACAACAATATATCAGTACGTGTTTATGAGTAAAGTAAATGTTCCCATGTTCATTAATATAATATATATGCTTCCAAAACACACCTTGATCATGTCATCGATCGTTTTGTTTATGTCTTTTTTTAACAAAGGTGGAGGTTCCGAAGTTGACGTTTCATTTCAAGGGCGCGGATTTGGAGCTGCCCGGGGAGAATTACATGATTGGGGATTCAAAAGCGGGATTGCTGTGCTTGGCCATTGGGAGTTCTAGAGGAATGTCCATCTTTGGAAATCTACAGCAACAAAATTTCATGGTTGTTCATGATCTTCAAGAAGAAACCCTATCGTTTTTGCCAACTCAATGTGATATTATATAA

mRNA sequence

ATGGCGGCTGTCTCGTTACGTTCATTTGGATATCTGCAGCTTCTTTTGCTTATTGTATTCATTACAACATTATTTATTAATACATTGGCGTTCAGTTCATCTCTATCAAGGCGAGCTCTACAGAAACCAAATAAGTTGCCTAGTCATGGCTTCAAGGTGAGGCTTAAACATGTGGATCATGTGAAGAATTTGACGAGATTCGAGCGGTTGCGGCGTGGGGTGGCACGTGGGAAAAATAGATTGCATAGACTAAATGCCATGGTGTTGGCCGCTGCCAACGCTACGGTTGGTGACCAAGTGAAGGCACCGGTGGTTGCAGGTAATGGTGAGTTTCTTATGAAGTTGGCCATCGGAAGTCCGCCGAGAAGCTTTTCGGCGATCATGGATACAGGCAGCGATCTGATATGGACACAATGCAAGCCTTGTCAACAGTGTTTTGATCAAGCAACACCTATTTTTGATCCCAAACAATCTTCTTCTTTCTCTAAGATTTCATGCTCGAGCGAGCTCTGTGGCGGTCTCCCAACGTCAACATGCAGTAGCGACGGGTGCGAGTATTTGTACACATATGGAGATTCTTCCTCCACCCAAGGTGTTTTGGCTTTTGAGACCTTCACCTTTGGAGATTCAACTGAAGATCAGATATCGATCCCTGGACTCGGGTTTGGATGCGGAAACGATAACAATGGAGATGGGTTTAGCCAAGGCGCAGGGCTAGTGGGGCTTGGGCGAGGACCCTTATCATTGGTTTCTCAACTAAAAGAGCAAAAGTTTGCTTATTGTTTAACCGCCATTGATGATTCAAAACCAAGCTCTCTTTTGTTAGGATCTCTAGCAAACATAACACCTAAAACATCAAAAGATGAAATGAAAACAACCCCATTGATAAAAAACCCTTCTCAACCATCCTTTTACTATCTTTCTCTCCAAGGAATCTCAGTTGGTGGAACTCAATTATCAATACCGAAGTCCACTTTTGAGCTCCATGATGATGGGAGTGGAGGAGTAATCATAGACTCAGGCACAACAATCACTTACATTGAGAACTCAGCTTTCACTTCACTCAAAAATGAGTTCATTGCTCAAATGAATCTTCCCGTTGACGATTCCGGTACCGGTGGTCTTGACCTCTGCTTTAACCTACCTGCCGGGACAAATCAGGTGGAGGTTCCGAAGTTGACGTTTCATTTCAAGGGCGCGGATTTGGAGCTGCCCGGGGAGAATTACATGATTGGGGATTCAAAAGCGGGATTGCTGTGCTTGGCCATTGGGAGTTCTAGAGGAATGTCCATCTTTGGAAATCTACAGCAACAAAATTTCATGGTTGTTCATGATCTTCAAGAAGAAACCCTATCGTTTTTGCCAACTCAATGTGATATTATATAA

Coding sequence (CDS)

ATGGCGGCTGTCTCGTTACGTTCATTTGGATATCTGCAGCTTCTTTTGCTTATTGTATTCATTACAACATTATTTATTAATACATTGGCGTTCAGTTCATCTCTATCAAGGCGAGCTCTACAGAAACCAAATAAGTTGCCTAGTCATGGCTTCAAGGTGAGGCTTAAACATGTGGATCATGTGAAGAATTTGACGAGATTCGAGCGGTTGCGGCGTGGGGTGGCACGTGGGAAAAATAGATTGCATAGACTAAATGCCATGGTGTTGGCCGCTGCCAACGCTACGGTTGGTGACCAAGTGAAGGCACCGGTGGTTGCAGGTAATGGTGAGTTTCTTATGAAGTTGGCCATCGGAAGTCCGCCGAGAAGCTTTTCGGCGATCATGGATACAGGCAGCGATCTGATATGGACACAATGCAAGCCTTGTCAACAGTGTTTTGATCAAGCAACACCTATTTTTGATCCCAAACAATCTTCTTCTTTCTCTAAGATTTCATGCTCGAGCGAGCTCTGTGGCGGTCTCCCAACGTCAACATGCAGTAGCGACGGGTGCGAGTATTTGTACACATATGGAGATTCTTCCTCCACCCAAGGTGTTTTGGCTTTTGAGACCTTCACCTTTGGAGATTCAACTGAAGATCAGATATCGATCCCTGGACTCGGGTTTGGATGCGGAAACGATAACAATGGAGATGGGTTTAGCCAAGGCGCAGGGCTAGTGGGGCTTGGGCGAGGACCCTTATCATTGGTTTCTCAACTAAAAGAGCAAAAGTTTGCTTATTGTTTAACCGCCATTGATGATTCAAAACCAAGCTCTCTTTTGTTAGGATCTCTAGCAAACATAACACCTAAAACATCAAAAGATGAAATGAAAACAACCCCATTGATAAAAAACCCTTCTCAACCATCCTTTTACTATCTTTCTCTCCAAGGAATCTCAGTTGGTGGAACTCAATTATCAATACCGAAGTCCACTTTTGAGCTCCATGATGATGGGAGTGGAGGAGTAATCATAGACTCAGGCACAACAATCACTTACATTGAGAACTCAGCTTTCACTTCACTCAAAAATGAGTTCATTGCTCAAATGAATCTTCCCGTTGACGATTCCGGTACCGGTGGTCTTGACCTCTGCTTTAACCTACCTGCCGGGACAAATCAGGTGGAGGTTCCGAAGTTGACGTTTCATTTCAAGGGCGCGGATTTGGAGCTGCCCGGGGAGAATTACATGATTGGGGATTCAAAAGCGGGATTGCTGTGCTTGGCCATTGGGAGTTCTAGAGGAATGTCCATCTTTGGAAATCTACAGCAACAAAATTTCATGGTTGTTCATGATCTTCAAGAAGAAACCCTATCGTTTTTGCCAACTCAATGTGATATTATATAA

Protein sequence

MAAVSLRSFGYLQLLLLIVFITTLFINTLAFSSSLSRRALQKPNKLPSHGFKVRLKHVDHVKNLTRFERLRRGVARGKNRLHRLNAMVLAAANATVGDQVKAPVVAGNGEFLMKLAIGSPPRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCSSELCGGLPTSTCSSDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLVGLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKTTPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIENSAFTSLKNEFIAQMNLPVDDSGTGGLDLCFNLPAGTNQVEVPKLTFHFKGADLELPGENYMIGDSKAGLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDII*

Homology

[Close]

BLAST of Chy8G146190 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 6.3e-119
Identity = 233/449 (51.89%), Postives = 305/449 (67.93%), Query Frame = 0


Query: 12  LQLLLLIVFITTLFINTLAFSSSLSRRALQKPNKLPSHGFKVRLKHVDHVKNLTRFERLR 71
           L   LL + I  +F+   A + S SR AL   ++    GF++ L+HVD  KNLT+F+ L 
Sbjct: 5   LYSFLLALSIVYIFV---APTHSTSRTALNHRHEAKVTGFQIMLEHVDSGKNLTKFQLLE 64

Query: 72  RGVARGKNRLHRLNAMVLAAANATVGDQVKAPVVAGNGEFLMKLAIGSPPRSFSAIMDTG 131
           R + RG  RL RL AM+    N   G  V+  V AG+GE+LM L+IG+P + FSAIMDTG
Sbjct: 65  RAIERGSRRLQRLEAML----NGPSG--VETSVYAGDGEYLMNLSIGTPAQPFSAIMDTG 124

Query: 132 SDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCSSELCGGLPTSTCSSDGCEYLYTYG 191
           SDLIWTQC+PC QCF+Q+TPIF+P+ SSSFS + CSS+LC  L + TCS++ C+Y Y YG
Sbjct: 125 SDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPCSSQLCQALSSPTCSNNFCQYTYGYG 184

Query: 192 DSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLVGLGRGPLSLVS 251
           D S TQG +  ET TFG      +SIP + FGCG +N G G   GAGLVG+GRGPLSL S
Sbjct: 185 DGSETQGSMGTETLTFG-----SVSIPNITFGCGENNQGFGQGNGAGLVGMGRGPLSLPS 244

Query: 252 QLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKTTPLIKNPSQPSFYYLSLQG 311
           QL   KF+YC+T I  S PS+LLLGSLAN     S +    T LI++   P+FYY++L G
Sbjct: 245 QLDVTKFSYCMTPIGSSTPSNLLLGSLANSVTAGSPN----TTLIQSSQIPTFYYITLNG 304

Query: 312 ISVGGTQLSIPKSTFELH-DDGSGGVIIDSGTTITYIENSAFTSLKNEFIAQMNLPVDDS 371
           +SVG T+L I  S F L+ ++G+GG+IIDSGTT+TY  N+A+ S++ EFI+Q+NLPV + 
Sbjct: 305 LSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQINLPVVNG 364

Query: 372 GTGGLDLCFNLPAGTNQVEVPKLTFHFKGADLELPGENYMIGDSKAGLLCLAIG-SSRGM 431
            + G DLCF  P+  + +++P    HF G DLELP ENY I  S  GL+CLA+G SS+GM
Sbjct: 365 SSSGFDLCFQTPSDPSNLQIPTFVMHFDGGDLELPSENYFISPSN-GLICLAMGSSSQGM 424

Query: 432 SIFGNLQQQNFMVVHDLQEETLSFLPTQC 459
           SIFGN+QQQN +VV+D     +SF   QC
Sbjct: 425 SIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

[Close]

BLAST of Chy8G146190 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 2.3e-113
Identity = 213/433 (49.19%), Postives = 294/433 (67.90%), Query Frame = 0


Query: 29  LAFSSSLSR-RALQKPNKLPSHGFKVRLKHVDHVKNLTRFERLRRGVARGKNRLHRLNAM 88
           +A +SS SR   L    K P  G +V L+ VD  KNLT++E ++R + RG+ R+  +NAM
Sbjct: 19  VAPTSSTSRGTLLHHGQKRPQPGLRVDLEQVDSGKNLTKYELIKRAIKRGERRMRSINAM 78

Query: 89  VLAAANATVGDQVKAPVVAGNGEFLMKLAIGSPPRSFSAIMDTGSDLIWTQCKPCQQCFD 148
           + +++       ++ PV AG+GE+LM +AIG+P  SFSAIMDTGSDLIWTQC+PC QCF 
Sbjct: 79  LQSSSG------IETPVYAGDGEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQCFS 138

Query: 149 QATPIFDPKQSSSFSKISCSSELCGGLPTSTCSSDGCEYLYTYGDSSSTQGVLAFETFTF 208
           Q TPIF+P+ SSSFS + C S+ C  LP+ TC+++ C+Y Y YGD S+TQG +A ETFTF
Sbjct: 139 QPTPIFNPQDSSSFSTLPCESQYCQDLPSETCNNNECQYTYGYGDGSTTQGYMATETFTF 198

Query: 209 GDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLVGLGRGPLSLVSQLKEQKFAYCLTAIDD 268
             S     S+P + FGCG DN G G   GAGL+G+G GPLSL SQL   +F+YC+T+   
Sbjct: 199 ETS-----SVPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYGS 258

Query: 269 SKPSSLLLGSLANITPKTSKDEMKTTPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFE 328
           S PS+L LGS A+  P+ S     +T LI +   P++YY++LQGI+VGG  L IP STF+
Sbjct: 259 SSPSTLALGSAASGVPEGS----PSTTLIHSSLNPTYYYITLQGITVGGDNLGIPSSTFQ 318

Query: 329 LHDDGSGGVIIDSGTTITYIENSAFTSLKNEFIAQMNLPVDDSGTGGLDLCFNLPAGTNQ 388
           L DDG+GG+IIDSGTT+TY+   A+ ++   F  Q+NLP  D  + GL  CF  P+  + 
Sbjct: 319 LQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQINLPTVDESSSGLSTCFQQPSDGST 378

Query: 389 VEVPKLTFHFKGADLELPGENYMIGDSKAGLLCLAIGSSR--GMSIFGNLQQQNFMVVHD 448
           V+VP+++  F G  L L  +N +I  ++ G++CLA+GSS   G+SIFGN+QQQ   V++D
Sbjct: 379 VQVPEISMQFDGGVLNLGEQNILISPAE-GVICLAMGSSSQLGISIFGNIQQQETQVLYD 435

Query: 449 LQEETLSFLPTQC 459
           LQ   +SF+PTQC
Sbjct: 439 LQNLAVSFVPTQC 435

[Close]

BLAST of Chy8G146190 vs. ExPASy Swiss-Prot
Match: Q6XBF8 (Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 5.4e-70
Identity = 160/444 (36.04%), Postives = 238/444 (53.60%), Query Frame = 0


Query: 21  ITTLFINTLAFSSSLSRRALQKPNKLPSHGFKVRLKHVDHVKNLTRFERLRRGVARGKNR 80
           + +LF + L     LS   L   N  P  GF   L H D  K+   +  +     R +N 
Sbjct: 1   MASLFSSVLLSLCLLSSLFLSNANAKPKLGFTADLIHRDSPKS-PFYNPMETSSQRLRNA 60

Query: 81  LHRLNAMVLAAANATVGDQVKAPVVAGNGEFLMKLAIGSPPRSFSAIMDTGSDLIWTQCK 140
           +HR    V          Q +  + + +GE+LM ++IG+PP    AI DTGSDL+WTQC 
Sbjct: 61  IHRSVNRVFHFTEKDNTPQPQIDLTSNSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCA 120

Query: 141 PCQQCFDQATPIFDPKQSSSFSKISCSSELCGGLPT-STCSSDG--CEYLYTYGDSSSTQ 200
           PC  C+ Q  P+FDPK SS++  +SCSS  C  L   ++CS++   C Y  +YGD+S T+
Sbjct: 121 PCDDCYTQVDPLFDPKTSSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSYGDNSYTK 180

Query: 201 GVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLVGLGRGPLSLVSQLKEQ- 260
           G +A +T T G S    + +  +  GCG++N G    +G+G+VGLG GP+SL+ QL +  
Sbjct: 181 GNIAVDTLTLGSSDTRPMQLKNIIIGCGHNNAGTFNKKGSGIVGLGGGPVSLIKQLGDSI 240

Query: 261 --KFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKTTPLIKNPSQPSFYYLSLQGISV 320
             KF+YCL  +   K  +  +    N     S   + +TPLI   SQ +FYYL+L+ ISV
Sbjct: 241 DGKFSYCLVPLTSKKDQTSKINFGTNAI--VSGSGVVSTPLIAKASQETFYYLTLKSISV 300

Query: 321 GGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIENSAFTSLKNEFIAQMNLPVDDSGTGG 380
           G  Q+    S  E      G +IIDSGTT+T +    ++ L++   + ++         G
Sbjct: 301 GSKQIQYSGSDSE---SSEGNIIIDSGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQSG 360

Query: 381 LDLCFNLPAGTNQVEVPKLTFHFKGADLELPGENYMIGDSKAGLLCLAIGSSRGMSIFGN 440
           L LC+   + T  ++VP +T HF GAD++L   N  +  S+  L+C A   S   SI+GN
Sbjct: 361 LSLCY---SATGDLKVPVITMHFDGADVKLDSSNAFVQVSE-DLVCFAFRGSPSFSIYGN 420

Query: 441 LQQQNFMVVHDLQEETLSFLPTQC 459
           + Q NF+V +D   +T+SF PT C
Sbjct: 421 VAQMNFLVGYDTVSKTVSFKPTDC 434

[Close]

BLAST of Chy8G146190 vs. ExPASy Swiss-Prot
Match: Q7XV21 (Aspartyl protease 37 OS=Oryza sativa subsp. japonica OX=39947 GN=AP37 PE=3 SV=2)

HSP 1 Score: 266.2 bits (679), Expect = 7.1e-70
Identity = 157/447 (35.12%), Postives = 237/447 (53.02%), Query Frame = 0


Query: 47  PSHGFKVRLKHVD----HVKNLTRFERLRRGVARGKNRLHRLNAMVLAAANATVGDQVKA 106
           P   F++ L  VD       NLT  E LRR + R + RL  +      AA+A      + 
Sbjct: 21  PPRSFRLELASVDASAADAANLTEHELLRRAIQRSRYRLAGIGMARGEAASARKAVVAET 80

Query: 107 PVVAGNGEFLMKLAIGSPPRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFS 166
           P++   GE+L+KL IG+PP  F+A +DT SDLIWTQC+PC  C+ Q  P+F+P+ SS+++
Sbjct: 81  PIMPAGGEYLVKLGIGTPPYKFTAAIDTASDLIWTQCQPCTGCYHQVDPMFNPRVSSTYA 140

Query: 167 KISCSSELCGGLPTSTCSSD---GCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPG 226
            + CSS+ C  L    C  D    C+Y YTY  +++T+G LA +    G+      +  G
Sbjct: 141 ALPCSSDTCDELDVHRCGHDDDESCQYTYTYSGNATTEGTLAVDKLVIGED-----AFRG 200

Query: 227 LGFGCGNDNNGDG-FSQGAGLVGLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSL 286
           + FGC   + G     Q +G+VGLGRGPLSLVSQL  ++FAYCL       P  L+LG+ 
Sbjct: 201 VAFGCSTSSTGGAPPPQASGVVGLGRGPLSLVSQLSVRRFAYCLPPPASRIPGKLVLGAD 260

Query: 287 ANITPKTSKDEMKTTPLIKNPSQPSFYYLSLQGISVGGTQLSIPKST------------- 346
           A+     +       P+ ++P  PS+YYL+L G+ +G   +S+P +T             
Sbjct: 261 ADAARNAT--NRIAVPMRRDPRYPSYYYLNLDGLLIGDRAMSLPPTTTTTATATATAPAP 320

Query: 347 ----------FELHDDGSGGVIIDSGTTITYIENSAFTSLKNEFIAQMNLPVDDSGTGGL 406
                       + D    G+IID  +TIT++E S +  L N+   ++ LP     + GL
Sbjct: 321 APTPSPNATAVAVGDANRYGMIIDIASTITFLEASLYDELVNDLEVEIRLPRGTGSSLGL 380

Query: 407 DLCFNLPAGT--NQVEVPKLTFHFKGADLELPGENYMIGDSKAGLLCLAIGSSR--GMSI 459
           DLCF LP G   ++V VP +   F G  L L        D ++G++CL +G +    +SI
Sbjct: 381 DLCFILPDGVAFDRVYVPAVALAFDGRWLRLDKARLFAEDRESGMMCLMVGRAEAGSVSI 440

[Close]

BLAST of Chy8G146190 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 5.6e-67
Identity = 138/368 (37.50%), Postives = 212/368 (57.61%), Query Frame = 0


Query: 98  DQVKAPVVA----GNGEFLMKLAIGSPPRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIF 157
           + +  PVV+    G+GE+  ++ +G+P +    ++DTGSD+ W QC+PC  C+ Q+ P+F
Sbjct: 145 EDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVF 204

Query: 158 DPKQSSSFSKISCSSELCGGLPTSTCSSDGCEYLYTYGDSSSTQGVLAFETFTFGDSTED 217
           +P  SS++  ++CS+  C  L TS C S+ C Y  +YGD S T G LA +T TFG+S + 
Sbjct: 205 NPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGDGSFTVGELATDTVTFGNSGK- 264

Query: 218 QISIPGLGFGCGNDNNGDGFSQGAGLVGLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSL 277
              I  +  GCG+DN G  F+  AGL+GLG G LS+ +Q+K   F+YCL   D  K SSL
Sbjct: 265 ---INNVALGCGHDNEG-LFTGAAGLLGLGGGVLSITNQMKATSFSYCLVDRDSGKSSSL 324

Query: 278 LLGSLANITPKTSKDEMKTTPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGS 337
              S+             T PL++N    +FYY+ L G SVGG ++ +P + F++   GS
Sbjct: 325 DFNSV------QLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDASGS 384

Query: 338 GGVIIDSGTTITYIENSAFTSLKNEFI-AQMNLPVDDSGTGGLDLCFNLPAGTNQVEVPK 397
           GGVI+D GT +T ++  A+ SL++ F+   +NL    S     D C++  +  + V+VP 
Sbjct: 385 GGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDF-SSLSTVKVPT 444

Query: 398 LTFHFKGA-DLELPGENYMIGDSKAGLLCLAIG-SSRGMSIFGNLQQQNFMVVHDLQEET 457
           + FHF G   L+LP +NY+I    +G  C A   +S  +SI GN+QQQ   + +DL +  
Sbjct: 445 VAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITYDLSKNV 500

Query: 458 LSFLPTQC 459
           +     +C
Sbjct: 505 IGLSGNKC 500

[Close]

BLAST of Chy8G146190 vs. ExPASy TrEMBL
Match: A0A0A0KYT9 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G554680 PE=3 SV=1)

HSP 1 Score: 892.9 bits (2306), Expect = 5.7e-256
Identity = 451/461 (97.83%), Postives = 455/461 (98.70%), Query Frame = 0


Query: 1   MAAVSLRSFGYLQLLLLIVFITTLFINTLAFSSSLSRRALQKPNKLPSHGFKVRLKHVDH 60
           MAAVSLRSFGYL  LLLI+ ITTLFINTLAFSSSLSRRALQKPNKLPSHGF+VRLKHVDH
Sbjct: 1   MAAVSLRSFGYLHRLLLIILITTLFINTLAFSSSLSRRALQKPNKLPSHGFRVRLKHVDH 60

Query: 61  VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANATVGDQVKAPVVAGNGEFLMKLAIGSP 120
           VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANATVGDQVKAPVVAGNGEFLMKLAIGSP
Sbjct: 61  VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANATVGDQVKAPVVAGNGEFLMKLAIGSP 120

Query: 121 PRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCSSELCGGLPTSTCS 180
           PRSFSAIMDTGSDLIWTQCKPCQQCFDQ+TPIFDPKQSSSF KISCSSELCG LPTSTCS
Sbjct: 121 PRSFSAIMDTGSDLIWTQCKPCQQCFDQSTPIFDPKQSSSFYKISCSSELCGALPTSTCS 180

Query: 181 SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV 240
           SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV
Sbjct: 181 SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV 240

Query: 241 GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKTTPLIKNPS 300
           GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKTTPLIKNPS
Sbjct: 241 GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKTTPLIKNPS 300

Query: 301 QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIENSAFTSLKNEFI 360
           QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITY+ENSAFTSLKNEFI
Sbjct: 301 QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYVENSAFTSLKNEFI 360

Query: 361 AQMNLPVDDSGTGGLDLCFNLPAGTNQVEVPKLTFHFKGADLELPGENYMIGDSKAGLLC 420
           AQMNLPVDDSGTGGLDLCFNLPAGTNQVEVPKLTFHFKGADLELPGENYMIGDSKAGLLC
Sbjct: 361 AQMNLPVDDSGTGGLDLCFNLPAGTNQVEVPKLTFHFKGADLELPGENYMIGDSKAGLLC 420

Query: 421 LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDII 462
           LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCD I
Sbjct: 421 LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461

[Close]

BLAST of Chy8G146190 vs. ExPASy TrEMBL
Match: A0A5D3BTY9 (Aspartic proteinase nepenthesin-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1738G00580 PE=3 SV=1)

HSP 1 Score: 888.6 bits (2295), Expect = 1.1e-254
Identity = 450/461 (97.61%), Postives = 454/461 (98.48%), Query Frame = 0


Query: 1   MAAVSLRSFGYLQLLLLIVFITTLFINTLAFSSSLSRRALQKPNKLPSHGFKVRLKHVDH 60
           MAAVSLRSFGYLQLLLLIVFITTLFINTLAFSSSLSRRALQKPNKLPSHGF VRLKHVDH
Sbjct: 1   MAAVSLRSFGYLQLLLLIVFITTLFINTLAFSSSLSRRALQKPNKLPSHGFMVRLKHVDH 60

Query: 61  VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANATVGDQVKAPVVAGNGEFLMKLAIGSP 120
           VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANA+VGDQVKAPVVAGNGEFLMKLAIGSP
Sbjct: 61  VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLAIGSP 120

Query: 121 PRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCSSELCGGLPTSTCS 180
           PRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISC SELCG LPTSTCS
Sbjct: 121 PRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPTSTCS 180

Query: 181 SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV 240
           SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV
Sbjct: 181 SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV 240

Query: 241 GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKTTPLIKNPS 300
           GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMK TPLIKNPS
Sbjct: 241 GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPS 300

Query: 301 QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIENSAFTSLKNEFI 360
           QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIE++AF+SLKNEFI
Sbjct: 301 QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFI 360

Query: 361 AQMNLPVDDSGTGGLDLCFNLPAGTNQVEVPKLTFHFKGADLELPGENYMIGDSKAGLLC 420
           AQMNLPVDDSGTGGLDLCFNLPAGT QVEVPKLTFHFKGADLELPGENYMIGDSK GLLC
Sbjct: 361 AQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLC 420

Query: 421 LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDII 462
           LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCD I
Sbjct: 421 LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461

[Close]

BLAST of Chy8G146190 vs. ExPASy TrEMBL
Match: A0A5A7TD10 (Aspartic proteinase nepenthesin-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold128G001020 PE=3 SV=1)

HSP 1 Score: 887.5 bits (2292), Expect = 2.4e-254
Identity = 449/461 (97.40%), Postives = 454/461 (98.48%), Query Frame = 0


Query: 1   MAAVSLRSFGYLQLLLLIVFITTLFINTLAFSSSLSRRALQKPNKLPSHGFKVRLKHVDH 60
           MAAVSLRSFGYLQLLLLIVFITTLFINTLAFSSSLS RALQKPNKLPSHGF+VRLKHVDH
Sbjct: 1   MAAVSLRSFGYLQLLLLIVFITTLFINTLAFSSSLSTRALQKPNKLPSHGFRVRLKHVDH 60

Query: 61  VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANATVGDQVKAPVVAGNGEFLMKLAIGSP 120
           VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANA+VGDQVKAPVVAGNGEFLMKLAIGSP
Sbjct: 61  VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLAIGSP 120

Query: 121 PRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCSSELCGGLPTSTCS 180
           PRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISC SELCG LPTSTCS
Sbjct: 121 PRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPTSTCS 180

Query: 181 SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV 240
           SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV
Sbjct: 181 SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV 240

Query: 241 GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKTTPLIKNPS 300
           GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMK TPLIKNPS
Sbjct: 241 GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPS 300

Query: 301 QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIENSAFTSLKNEFI 360
           QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIE++AF+SLKNEFI
Sbjct: 301 QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFI 360

Query: 361 AQMNLPVDDSGTGGLDLCFNLPAGTNQVEVPKLTFHFKGADLELPGENYMIGDSKAGLLC 420
           AQMNLPVDDSGTGGLDLCFNLPAGT QVEVPKLTFHFKGADLELPGENYMIGDSK GLLC
Sbjct: 361 AQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLC 420

Query: 421 LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDII 462
           LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCD I
Sbjct: 421 LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461

[Close]

BLAST of Chy8G146190 vs. ExPASy TrEMBL
Match: A0A1S3B573 (aspartic proteinase nepenthesin-1 OS=Cucumis melo OX=3656 GN=LOC103486136 PE=3 SV=1)

HSP 1 Score: 887.5 bits (2292), Expect = 2.4e-254
Identity = 449/461 (97.40%), Postives = 454/461 (98.48%), Query Frame = 0


Query: 1   MAAVSLRSFGYLQLLLLIVFITTLFINTLAFSSSLSRRALQKPNKLPSHGFKVRLKHVDH 60
           MAAVSLRSFGYLQLLLLIVFITTLFINTLAFSSSLS RALQKPNKLPSHGF+VRLKHVDH
Sbjct: 1   MAAVSLRSFGYLQLLLLIVFITTLFINTLAFSSSLSTRALQKPNKLPSHGFRVRLKHVDH 60

Query: 61  VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANATVGDQVKAPVVAGNGEFLMKLAIGSP 120
           VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANA+VGDQVKAPVVAGNGEFLMKLAIGSP
Sbjct: 61  VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLAIGSP 120

Query: 121 PRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCSSELCGGLPTSTCS 180
           PRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISC SELCG LPTSTCS
Sbjct: 121 PRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPTSTCS 180

Query: 181 SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV 240
           SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV
Sbjct: 181 SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV 240

Query: 241 GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKTTPLIKNPS 300
           GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMK TPLIKNPS
Sbjct: 241 GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPS 300

Query: 301 QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIENSAFTSLKNEFI 360
           QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIE++AF+SLKNEFI
Sbjct: 301 QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFI 360

Query: 361 AQMNLPVDDSGTGGLDLCFNLPAGTNQVEVPKLTFHFKGADLELPGENYMIGDSKAGLLC 420
           AQMNLPVDDSGTGGLDLCFNLPAGT QVEVPKLTFHFKGADLELPGENYMIGDSK GLLC
Sbjct: 361 AQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLC 420

Query: 421 LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDII 462
           LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCD I
Sbjct: 421 LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461

[Close]

BLAST of Chy8G146190 vs. ExPASy TrEMBL
Match: A0A6J1HXS0 (aspartic proteinase nepenthesin-1 OS=Cucurbita maxima OX=3661 GN=LOC111467205 PE=3 SV=1)

HSP 1 Score: 746.5 bits (1926), Expect = 6.6e-212
Identity = 381/454 (83.92%), Postives = 410/454 (90.31%), Query Frame = 0


Query: 8   SFGYLQLLLLIVFITTLFINTLAFSSSLSRRALQKPNKLPSHGFKVRLKHVDHVKNLTRF 67
           S  YL ++ +++ IT LFI+T A SSSLSRRAL++P KLPS GF+V L HVDHVKNLTRF
Sbjct: 4   SLRYL-IVSVVLSITMLFIHTSASSSSLSRRALRQP-KLPSDGFRVSLNHVDHVKNLTRF 63

Query: 68  ERLRRGVARGKNRLHRLNAMVLAAANATVGDQVKAPVVAGNGEFLMKLAIGSPPRSFSAI 127
           ERL+RGVARGK RLHRLNAM+L AAN  VG +V+APVVAGNGEFLMKLAIGSPPRSFSAI
Sbjct: 64  ERLQRGVARGKTRLHRLNAMML-AANIGVGGRVQAPVVAGNGEFLMKLAIGSPPRSFSAI 123

Query: 128 MDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCSSELCGGLPTSTCSSDGCEYL 187
           MDTGSDLIWTQCKPCQQCFDQATPIFDPK+SSSFSKISCSSELC  LPTSTCSSD CEY 
Sbjct: 124 MDTGSDLIWTQCKPCQQCFDQATPIFDPKESSSFSKISCSSELCDALPTSTCSSDECEYF 183

Query: 188 YTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLVGLGRGPL 247
           YTYGD SST GVLA ETFTFGDS++DQ+SIPGLGFGCG+DN GDGFSQG GLVGLGRGPL
Sbjct: 184 YTYGDYSSTHGVLAAETFTFGDSSQDQVSIPGLGFGCGDDNEGDGFSQGEGLVGLGRGPL 243

Query: 248 SLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKTTPLIKNPSQPSFYYL 307
           SLVSQLKEQKF+YCLTAIDD+KPSSLLLGSLAN+ PK S  E+KTTPLI+NPSQPSFYYL
Sbjct: 244 SLVSQLKEQKFSYCLTAIDDTKPSSLLLGSLANVKPKASDGEIKTTPLIRNPSQPSFYYL 303

Query: 308 SLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIENSAFTSLKNEFIAQMNLPV 367
           SLQGISVGGTQL IPK+TFELHDDGSGGVIIDSGTTITYIE +AFT LK EF++QM LPV
Sbjct: 304 SLQGISVGGTQLPIPKNTFELHDDGSGGVIIDSGTTITYIEKNAFTLLKKEFVSQMKLPV 363

Query: 368 DDSGTGGLDLCFNLPAGTNQVEVPKLTFHFKGADLELPGENYMIGDSKAGLLCLAIGSSR 427
           DDSGT GLDLCFNLP  TNQVEVPKLTFHFKGADLELPGENYMIGDSKA L+CL IGSS 
Sbjct: 364 DDSGTSGLDLCFNLPPKTNQVEVPKLTFHFKGADLELPGENYMIGDSKAELICLTIGSSN 423

Query: 428 GMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDII 462
           GMSIFGNLQQQN MVVHDLQEET+SFLPTQC  I
Sbjct: 424 GMSIFGNLQQQNIMVVHDLQEETVSFLPTQCSDI 454

[Close]

BLAST of Chy8G146190 vs. NCBI nr
Match: XP_011653928.1 (aspartic proteinase nepenthesin-1 [Cucumis sativus] >KGN54860.1 hypothetical protein Csa_012038 [Cucumis sativus])

HSP 1 Score: 890 bits (2300), Expect = 0.0
Identity = 451/461 (97.83%), Postives = 455/461 (98.70%), Query Frame = 0


Query: 1   MAAVSLRSFGYLQLLLLIVFITTLFINTLAFSSSLSRRALQKPNKLPSHGFKVRLKHVDH 60
           MAAVSLRSFGYL  LLLI+ ITTLFINTLAFSSSLSRRALQKPNKLPSHGF+VRLKHVDH
Sbjct: 1   MAAVSLRSFGYLHRLLLIILITTLFINTLAFSSSLSRRALQKPNKLPSHGFRVRLKHVDH 60

Query: 61  VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANATVGDQVKAPVVAGNGEFLMKLAIGSP 120
           VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANATVGDQVKAPVVAGNGEFLMKLAIGSP
Sbjct: 61  VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANATVGDQVKAPVVAGNGEFLMKLAIGSP 120

Query: 121 PRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCSSELCGGLPTSTCS 180
           PRSFSAIMDTGSDLIWTQCKPCQQCFDQ+TPIFDPKQSSSF KISCSSELCG LPTSTCS
Sbjct: 121 PRSFSAIMDTGSDLIWTQCKPCQQCFDQSTPIFDPKQSSSFYKISCSSELCGALPTSTCS 180

Query: 181 SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV 240
           SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV
Sbjct: 181 SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV 240

Query: 241 GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKTTPLIKNPS 300
           GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKTTPLIKNPS
Sbjct: 241 GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKTTPLIKNPS 300

Query: 301 QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIENSAFTSLKNEFI 360
           QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITY+ENSAFTSLKNEFI
Sbjct: 301 QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYVENSAFTSLKNEFI 360

Query: 361 AQMNLPVDDSGTGGLDLCFNLPAGTNQVEVPKLTFHFKGADLELPGENYMIGDSKAGLLC 420
           AQMNLPVDDSGTGGLDLCFNLPAGTNQVEVPKLTFHFKGADLELPGENYMIGDSKAGLLC
Sbjct: 361 AQMNLPVDDSGTGGLDLCFNLPAGTNQVEVPKLTFHFKGADLELPGENYMIGDSKAGLLC 420

Query: 421 LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDII 461
           LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCD I
Sbjct: 421 LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461

[Close]

BLAST of Chy8G146190 vs. NCBI nr
Match: TYK02448.1 (aspartic proteinase nepenthesin-1 [Cucumis melo var. makuwa])

HSP 1 Score: 886 bits (2289), Expect = 0.0
Identity = 450/461 (97.61%), Postives = 454/461 (98.48%), Query Frame = 0


Query: 1   MAAVSLRSFGYLQLLLLIVFITTLFINTLAFSSSLSRRALQKPNKLPSHGFKVRLKHVDH 60
           MAAVSLRSFGYLQLLLLIVFITTLFINTLAFSSSLSRRALQKPNKLPSHGF VRLKHVDH
Sbjct: 1   MAAVSLRSFGYLQLLLLIVFITTLFINTLAFSSSLSRRALQKPNKLPSHGFMVRLKHVDH 60

Query: 61  VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANATVGDQVKAPVVAGNGEFLMKLAIGSP 120
           VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANA+VGDQVKAPVVAGNGEFLMKLAIGSP
Sbjct: 61  VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLAIGSP 120

Query: 121 PRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCSSELCGGLPTSTCS 180
           PRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISC SELCG LPTSTCS
Sbjct: 121 PRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPTSTCS 180

Query: 181 SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV 240
           SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV
Sbjct: 181 SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV 240

Query: 241 GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKTTPLIKNPS 300
           GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMK TPLIKNPS
Sbjct: 241 GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPS 300

Query: 301 QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIENSAFTSLKNEFI 360
           QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIE++AF+SLKNEFI
Sbjct: 301 QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFI 360

Query: 361 AQMNLPVDDSGTGGLDLCFNLPAGTNQVEVPKLTFHFKGADLELPGENYMIGDSKAGLLC 420
           AQMNLPVDDSGTGGLDLCFNLPAGT QVEVPKLTFHFKGADLELPGENYMIGDSK GLLC
Sbjct: 361 AQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLC 420

Query: 421 LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDII 461
           LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCD I
Sbjct: 421 LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461

[Close]

BLAST of Chy8G146190 vs. NCBI nr
Match: XP_008442220.1 (PREDICTED: aspartic proteinase nepenthesin-1 [Cucumis melo] >KAA0041170.1 aspartic proteinase nepenthesin-1 [Cucumis melo var. makuwa])

HSP 1 Score: 885 bits (2286), Expect = 0.0
Identity = 449/461 (97.40%), Postives = 454/461 (98.48%), Query Frame = 0


Query: 1   MAAVSLRSFGYLQLLLLIVFITTLFINTLAFSSSLSRRALQKPNKLPSHGFKVRLKHVDH 60
           MAAVSLRSFGYLQLLLLIVFITTLFINTLAFSSSLS RALQKPNKLPSHGF+VRLKHVDH
Sbjct: 1   MAAVSLRSFGYLQLLLLIVFITTLFINTLAFSSSLSTRALQKPNKLPSHGFRVRLKHVDH 60

Query: 61  VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANATVGDQVKAPVVAGNGEFLMKLAIGSP 120
           VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANA+VGDQVKAPVVAGNGEFLMKLAIGSP
Sbjct: 61  VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLAIGSP 120

Query: 121 PRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCSSELCGGLPTSTCS 180
           PRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISC SELCG LPTSTCS
Sbjct: 121 PRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPTSTCS 180

Query: 181 SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV 240
           SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV
Sbjct: 181 SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV 240

Query: 241 GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKTTPLIKNPS 300
           GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMK TPLIKNPS
Sbjct: 241 GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPS 300

Query: 301 QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIENSAFTSLKNEFI 360
           QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIE++AF+SLKNEFI
Sbjct: 301 QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFI 360

Query: 361 AQMNLPVDDSGTGGLDLCFNLPAGTNQVEVPKLTFHFKGADLELPGENYMIGDSKAGLLC 420
           AQMNLPVDDSGTGGLDLCFNLPAGT QVEVPKLTFHFKGADLELPGENYMIGDSK GLLC
Sbjct: 361 AQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLC 420

Query: 421 LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDII 461
           LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCD I
Sbjct: 421 LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461

[Close]

BLAST of Chy8G146190 vs. NCBI nr
Match: XP_038883313.1 (aspartic proteinase nepenthesin-1 [Benincasa hispida])

HSP 1 Score: 799 bits (2063), Expect = 1.08e-289
Identity = 410/458 (89.52%), Postives = 432/458 (94.32%), Query Frame = 0


Query: 4   VSLRSFGYLQLLLLIVFITTLFINTLAFSSSLSRRALQKPNKLPSHGFKVRLKHVDHVKN 63
           VSL S+  L +LL+IV ITTLFI+TLA    LSRRALQK N+LPS+GFKV+L HVDHVKN
Sbjct: 3   VSLHSYRCL-ILLVIVLITTLFIDTLA----LSRRALQKSNELPSNGFKVKLNHVDHVKN 62

Query: 64  LTRFERLRRGVARGKNRLHRLNAMVLAAANATVGDQVKAPVVAGNGEFLMKLAIGSPPRS 123
           LTRFERLRRGVARGKNRLHRLNAMVLAA NA VG+QV+APVVAGNGEFLMKLAIG+PP+S
Sbjct: 63  LTRFERLRRGVARGKNRLHRLNAMVLAA-NAAVGEQVQAPVVAGNGEFLMKLAIGTPPKS 122

Query: 124 FSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCSSELCGGLPTSTCSSDG 183
           FSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPK SSSFSKISCSSELCG LPTSTCSSDG
Sbjct: 123 FSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKASSSFSKISCSSELCGPLPTSTCSSDG 182

Query: 184 CEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLVGLG 243
           CEYLYTYGDSSSTQGVLA ETFTFGDS++DQ+SI GLGFGCG+DN GDGFSQGAGLVGLG
Sbjct: 183 CEYLYTYGDSSSTQGVLALETFTFGDSSDDQVSITGLGFGCGDDNEGDGFSQGAGLVGLG 242

Query: 244 RGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKTTPLIKNPSQPS 303
           RGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANI PKT+KDEMKTTPLI+NPSQPS
Sbjct: 243 RGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANIKPKTTKDEMKTTPLIRNPSQPS 302

Query: 304 FYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIENSAFTSLKNEFIAQM 363
           FYYLSLQGISVG TQLSIPK+TFELHDDGSGGVIIDSGTTITYIEN+AFT LKNEFIAQM
Sbjct: 303 FYYLSLQGISVGSTQLSIPKTTFELHDDGSGGVIIDSGTTITYIENTAFTLLKNEFIAQM 362

Query: 364 NLPVDDSGTGGLDLCFNLPAGTNQVEVPKLTFHFKGADLELPGENYMIGDSKAGLLCLAI 423
           NLPVDDSGTGGLDLCFNLPA   QVEVPKLTFHF+GADLELPGENYMIGDS+ GL+CLAI
Sbjct: 363 NLPVDDSGTGGLDLCFNLPAEATQVEVPKLTFHFEGADLELPGENYMIGDSRTGLICLAI 422

Query: 424 GSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDII 461
           GSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCD I
Sbjct: 423 GSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 454

[Close]

BLAST of Chy8G146190 vs. NCBI nr
Match: XP_022967799.1 (aspartic proteinase nepenthesin-1 [Cucurbita maxima])

HSP 1 Score: 744 bits (1922), Expect = 3.20e-268
Identity = 378/441 (85.71%), Postives = 402/441 (91.16%), Query Frame = 0


Query: 21  ITTLFINTLAFSSSLSRRALQKPNKLPSHGFKVRLKHVDHVKNLTRFERLRRGVARGKNR 80
           IT LFI+T A SSSLSRRAL++P KLPS GF+V L HVDHVKNLTRFERL+RGVARGK R
Sbjct: 16  ITMLFIHTSASSSSLSRRALRQP-KLPSDGFRVSLNHVDHVKNLTRFERLQRGVARGKTR 75

Query: 81  LHRLNAMVLAAANATVGDQVKAPVVAGNGEFLMKLAIGSPPRSFSAIMDTGSDLIWTQCK 140
           LHRLNAM+LAA N  VG +V+APVVAGNGEFLMKLAIGSPPRSFSAIMDTGSDLIWTQCK
Sbjct: 76  LHRLNAMMLAA-NIGVGGRVQAPVVAGNGEFLMKLAIGSPPRSFSAIMDTGSDLIWTQCK 135

Query: 141 PCQQCFDQATPIFDPKQSSSFSKISCSSELCGGLPTSTCSSDGCEYLYTYGDSSSTQGVL 200
           PCQQCFDQATPIFDPK+SSSFSKISCSSELC  LPTSTCSSD CEY YTYGD SST GVL
Sbjct: 136 PCQQCFDQATPIFDPKESSSFSKISCSSELCDALPTSTCSSDECEYFYTYGDYSSTHGVL 195

Query: 201 AFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLVGLGRGPLSLVSQLKEQKFAY 260
           A ETFTFGDS++DQ+SIPGLGFGCG+DN GDGFSQG GLVGLGRGPLSLVSQLKEQKF+Y
Sbjct: 196 AAETFTFGDSSQDQVSIPGLGFGCGDDNEGDGFSQGEGLVGLGRGPLSLVSQLKEQKFSY 255

Query: 261 CLTAIDDSKPSSLLLGSLANITPKTSKDEMKTTPLIKNPSQPSFYYLSLQGISVGGTQLS 320
           CLTAIDD+KPSSLLLGSLAN+ PK S  E+KTTPLI+NPSQPSFYYLSLQGISVGGTQL 
Sbjct: 256 CLTAIDDTKPSSLLLGSLANVKPKASDGEIKTTPLIRNPSQPSFYYLSLQGISVGGTQLP 315

Query: 321 IPKSTFELHDDGSGGVIIDSGTTITYIENSAFTSLKNEFIAQMNLPVDDSGTGGLDLCFN 380
           IPK+TFELHDDGSGGVIIDSGTTITYIE +AFT LK EF++QM LPVDDSGT GLDLCFN
Sbjct: 316 IPKNTFELHDDGSGGVIIDSGTTITYIEKNAFTLLKKEFVSQMKLPVDDSGTSGLDLCFN 375

Query: 381 LPAGTNQVEVPKLTFHFKGADLELPGENYMIGDSKAGLLCLAIGSSRGMSIFGNLQQQNF 440
           LP  TNQVEVPKLTFHFKGADLELPGENYMIGDSKA L+CL IGSS GMSIFGNLQQQN 
Sbjct: 376 LPPKTNQVEVPKLTFHFKGADLELPGENYMIGDSKAELICLTIGSSNGMSIFGNLQQQNI 435

Query: 441 MVVHDLQEETLSFLPTQCDII 461
           MVVHDLQEET+SFLPTQC  I
Sbjct: 436 MVVHDLQEETVSFLPTQCSDI 454

[Close]

BLAST of Chy8G146190 vs. TAIR 10
Match: AT2G03200.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 538.1 bits (1385), Expect = 6.9e-153
Identity = 279/456 (61.18%), Postives = 349/456 (76.54%), Query Frame = 0


Query: 16  LLIVFITTLFINTLAFSSSLSRRAL---QKPNKLPSHGFKVRLKHVDHVKNLTRFERLRR 75
           LL  F   LF   ++ SS  SRR+L     P  LP  GF++ L+HVD  KNLT+ ++++R
Sbjct: 9   LLFPFFLILFSCLISVSS--SRRSLIDRTLPKNLPRSGFRLSLRHVDSGKNLTKIQKIQR 68

Query: 76  GVARGKNRLHRLNAMVLAAANATVGD--QVKAPVVAGNGEFLMKLAIGSPPRSFSAIMDT 135
           G+ RG +RL+RL A+ + A  +   D   +KAP   G+GEFLM+L+IG+P   +SAI+DT
Sbjct: 69  GINRGFHRLNRLGAVAVLAVASKPDDTNNIKAPTHGGSGEFLMELSIGNPAVKYSAIVDT 128

Query: 136 GSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCSSELCGGLPTSTCS--SDGCEYLY 195
           GSDLIWTQCKPC +CFDQ TPIFDP++SSS+SK+ CSS LC  LP S C+   D CEYLY
Sbjct: 129 GSDLIWTQCKPCTECFDQPTPIFDPEKSSSYSKVGCSSGLCNALPRSNCNEDKDACEYLY 188

Query: 196 TYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLVGLGRGPLS 255
           TYGD SST+G+LA ETFTF    ED+ SI G+GFGCG +N GDGFSQG+GLVGLGRGPLS
Sbjct: 189 TYGDYSSTRGLLATETFTF----EDENSISGIGFGCGVENEGDGFSQGSGLVGLGRGPLS 248

Query: 256 LVSQLKEQKFAYCLTAIDDSK-PSSLLLGSLAN-ITPKTSK----DEMKTTPLIKNPSQP 315
           L+SQLKE KF+YCLT+I+DS+  SSL +GSLA+ I  KT      +  KT  L++NP QP
Sbjct: 249 LISQLKETKFSYCLTSIEDSEASSSLFIGSLASGIVNKTGASLDGEVTKTMSLLRNPDQP 308

Query: 316 SFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIENSAFTSLKNEFIAQ 375
           SFYYL LQGI+VG  +LS+ KSTFEL +DG+GG+IIDSGTTITY+E +AF  LK EF ++
Sbjct: 309 SFYYLELQGITVGAKRLSVEKSTFELAEDGTGGMIIDSGTTITYLEETAFKVLKEEFTSR 368

Query: 376 MNLPVDDSGTGGLDLCFNLPAGTNQVEVPKLTFHFKGADLELPGENYMIGDSKAGLLCLA 435
           M+LPVDDSG+ GLDLCF LP     + VPK+ FHFKGADLELPGENYM+ DS  G+LCLA
Sbjct: 369 MSLPVDDSGSTGLDLCFKLPDAAKNIAVPKMIFHFKGADLELPGENYMVADSSTGVLCLA 428

Query: 436 IGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQC 459
           +GSS GMSIFGN+QQQNF V+HDL++ET+SF+PT+C
Sbjct: 429 MGSSNGMSIFGNVQQQNFNVLHDLEKETVSFVPTEC 458

[Close]

BLAST of Chy8G146190 vs. TAIR 10
Match: AT5G33340.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 266.5 bits (680), Expect = 3.9e-71
Identity = 160/444 (36.04%), Postives = 238/444 (53.60%), Query Frame = 0


Query: 21  ITTLFINTLAFSSSLSRRALQKPNKLPSHGFKVRLKHVDHVKNLTRFERLRRGVARGKNR 80
           + +LF + L     LS   L   N  P  GF   L H D  K+   +  +     R +N 
Sbjct: 1   MASLFSSVLLSLCLLSSLFLSNANAKPKLGFTADLIHRDSPKS-PFYNPMETSSQRLRNA 60

Query: 81  LHRLNAMVLAAANATVGDQVKAPVVAGNGEFLMKLAIGSPPRSFSAIMDTGSDLIWTQCK 140
           +HR    V          Q +  + + +GE+LM ++IG+PP    AI DTGSDL+WTQC 
Sbjct: 61  IHRSVNRVFHFTEKDNTPQPQIDLTSNSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCA 120

Query: 141 PCQQCFDQATPIFDPKQSSSFSKISCSSELCGGLPT-STCSSDG--CEYLYTYGDSSSTQ 200
           PC  C+ Q  P+FDPK SS++  +SCSS  C  L   ++CS++   C Y  +YGD+S T+
Sbjct: 121 PCDDCYTQVDPLFDPKTSSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSYGDNSYTK 180

Query: 201 GVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLVGLGRGPLSLVSQLKEQ- 260
           G +A +T T G S    + +  +  GCG++N G    +G+G+VGLG GP+SL+ QL +  
Sbjct: 181 GNIAVDTLTLGSSDTRPMQLKNIIIGCGHNNAGTFNKKGSGIVGLGGGPVSLIKQLGDSI 240

Query: 261 --KFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKTTPLIKNPSQPSFYYLSLQGISV 320
             KF+YCL  +   K  +  +    N     S   + +TPLI   SQ +FYYL+L+ ISV
Sbjct: 241 DGKFSYCLVPLTSKKDQTSKINFGTNAI--VSGSGVVSTPLIAKASQETFYYLTLKSISV 300

Query: 321 GGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIENSAFTSLKNEFIAQMNLPVDDSGTGG 380
           G  Q+    S  E      G +IIDSGTT+T +    ++ L++   + ++         G
Sbjct: 301 GSKQIQYSGSDSE---SSEGNIIIDSGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQSG 360

Query: 381 LDLCFNLPAGTNQVEVPKLTFHFKGADLELPGENYMIGDSKAGLLCLAIGSSRGMSIFGN 440
           L LC+   + T  ++VP +T HF GAD++L   N  +  S+  L+C A   S   SI+GN
Sbjct: 361 LSLCY---SATGDLKVPVITMHFDGADVKLDSSNAFVQVSE-DLVCFAFRGSPSFSIYGN 420

Query: 441 LQQQNFMVVHDLQEETLSFLPTQC 459
           + Q NF+V +D   +T+SF PT C
Sbjct: 421 VAQMNFLVGYDTVSKTVSFKPTDC 434

[Close]

BLAST of Chy8G146190 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 263.5 bits (672), Expect = 3.3e-70
Identity = 164/458 (35.81%), Postives = 240/458 (52.40%), Query Frame = 0


Query: 26  INTLAFSSSLSRRALQKPNKLPSHGFKVRL---------KHVDHVKNLTRFERLRRGVAR 85
           I+   ++SS      ++     S  F ++L         +H D+ K+LT   RL R  AR
Sbjct: 42  IHRTKYTSSFRLNQQEEQTHSASSSFSLQLHSRVSVRGTEHSDY-KSLT-LARLNRDTAR 101

Query: 86  GKNRLHRLNAMV----------LAAANATVGDQVKAPVVA----GNGEFLMKLAIGSPPR 145
            K+ + RL+  +          ++    T    ++AP+++    G+GE+  ++ IG P R
Sbjct: 102 VKSLITRLDLAINNISKADLKPISTMYTTEEQDIEAPLISGTTQGSGEYFTRVGIGKPAR 161

Query: 146 SFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCSSELCGGLPTSTCSSD 205
               ++DTGSD+ W QC PC  C+ Q  PIF+P  SSS+  +SC +  C  L  S C + 
Sbjct: 162 EVYMVLDTGSDVNWLQCTPCADCYHQTEPIFEPSSSSSYEPLSCDTPQCNALEVSECRNA 221

Query: 206 GCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLVGL 265
            C Y  +YGD S T G  A ET T G +    +++     GCG+ N G  F   AGL+GL
Sbjct: 222 TCLYEVSYGDGSYTVGDFATETLTIGSTLVQNVAV-----GCGHSNEG-LFVGAAGLLGL 281

Query: 266 GRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKTTPLIKNPSQP 325
           G G L+L SQL    F+YCL   D    S++  G+  +++P     +    PL++N    
Sbjct: 282 GGGLLALPSQLNTTSFSYCLVDRDSDSASTVDFGT--SLSP-----DAVVAPLLRNHQLD 341

Query: 326 SFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIENSAFTSLKNEFIAQ 385
           +FYYL L GISVGG  L IP+S+FE+ + GSGG+IIDSGT +T ++   + SL++ F+  
Sbjct: 342 TFYYLGLTGISVGGELLQIPQSSFEMDESGSGGIIIDSGTAVTRLQTEIYNSLRDSFVKG 401

Query: 386 MNLPVDDSGTGGLDLCFNLPAGTNQVEVPKLTFHFKGAD-LELPGENYMIGDSKAGLLCL 445
                  +G    D C+NL A T  VEVP + FHF G   L LP +NYMI     G  CL
Sbjct: 402 TLDLEKAAGVAMFDTCYNLSAKTT-VEVPTVAFHFPGGKMLALPAKNYMIPVDSVGTFCL 461

Query: 446 AIG-SSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQC 459
           A   ++  ++I GN+QQQ   V  DL    + F   +C
Sbjct: 462 AFAPTASSLAIIGNVQQQGTRVTFDLANSLIGFSSNKC 483

[Close]

BLAST of Chy8G146190 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 256.5 bits (654), Expect = 4.0e-68
Identity = 138/368 (37.50%), Postives = 212/368 (57.61%), Query Frame = 0


Query: 98  DQVKAPVVA----GNGEFLMKLAIGSPPRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIF 157
           + +  PVV+    G+GE+  ++ +G+P +    ++DTGSD+ W QC+PC  C+ Q+ P+F
Sbjct: 145 EDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVF 204

Query: 158 DPKQSSSFSKISCSSELCGGLPTSTCSSDGCEYLYTYGDSSSTQGVLAFETFTFGDSTED 217
           +P  SS++  ++CS+  C  L TS C S+ C Y  +YGD S T G LA +T TFG+S + 
Sbjct: 205 NPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGDGSFTVGELATDTVTFGNSGK- 264

Query: 218 QISIPGLGFGCGNDNNGDGFSQGAGLVGLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSL 277
              I  +  GCG+DN G  F+  AGL+GLG G LS+ +Q+K   F+YCL   D  K SSL
Sbjct: 265 ---INNVALGCGHDNEG-LFTGAAGLLGLGGGVLSITNQMKATSFSYCLVDRDSGKSSSL 324

Query: 278 LLGSLANITPKTSKDEMKTTPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGS 337
              S+             T PL++N    +FYY+ L G SVGG ++ +P + F++   GS
Sbjct: 325 DFNSV------QLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDASGS 384

Query: 338 GGVIIDSGTTITYIENSAFTSLKNEFI-AQMNLPVDDSGTGGLDLCFNLPAGTNQVEVPK 397
           GGVI+D GT +T ++  A+ SL++ F+   +NL    S     D C++  +  + V+VP 
Sbjct: 385 GGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDF-SSLSTVKVPT 444

Query: 398 LTFHFKGA-DLELPGENYMIGDSKAGLLCLAIG-SSRGMSIFGNLQQQNFMVVHDLQEET 457
           + FHF G   L+LP +NY+I    +G  C A   +S  +SI GN+QQQ   + +DL +  
Sbjct: 445 VAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITYDLSKNV 500

Query: 458 LSFLPTQC 459
           +     +C
Sbjct: 505 IGLSGNKC 500

[Close]

BLAST of Chy8G146190 vs. TAIR 10
Match: AT1G64830.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 255.8 bits (652), Expect = 6.8e-68
Identity = 154/439 (35.08%), Postives = 238/439 (54.21%), Query Frame = 0


Query: 28  TLAFSSSLSRRALQKPNKLPSHGFKVRLKHVDHVKNLTRFERLRRGVARGKNRLHRLNAM 87
           +L F++ LS   L   N  P  GF + L H D  K+   +        R +N + R    
Sbjct: 3   SLIFATLLSLLLLSNVNAYPKDGFTIDLIHRDSPKS-PFYNSAETSSQRMRNAIRRSARS 62

Query: 88  VLAAANATVGDQVKAPVVAGN-GEFLMKLAIGSPPRSFSAIMDTGSDLIWTQCKPCQQCF 147
            L  +N           +  N GE+LM ++IG+PP    AI DTGSDLIWTQC PC+ C+
Sbjct: 63  TLQFSNDDASPNSPQSFITSNRGEYLMNISIGTPPVPILAIADTGSDLIWTQCNPCEDCY 122

Query: 148 DQATPIFDPKQSSSFSKISCSSELCGGLPTSTCSSD--GCEYLYTYGDSSSTQGVLAFET 207
            Q +P+FDPK+SS++ K+SCSS  C  L  ++CS+D   C Y  TYGD+S T+G +A +T
Sbjct: 123 QQTSPLFDPKESSTYRKVSCSSSQCRALEDASCSTDENTCSYTITYGDNSYTKGDVAVDT 182

Query: 208 FTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLVGLGRGPLSLVSQLKEQ---KFAYC 267
            T G S    +S+  +  GCG++N G     G+G++GLG G  SLVSQL++    KF+YC
Sbjct: 183 VTMGSSGRRPVSLRNMIIGCGHENTGTFDPAGSGIIGLGGGSTSLVSQLRKSINGKFSYC 242

Query: 268 LTAI--DDSKPSSLLLGSLANITPKTSKDEMKTTPLIKNPSQPSFYYLSLQGISVGGTQL 327
           L     +    S +  G+   +    S D + +T ++K     ++Y+L+L+ ISVG  ++
Sbjct: 243 LVPFTSETGLTSKINFGTNGIV----SGDGVVSTSMVKK-DPATYYFLNLEAISVGSKKI 302

Query: 328 SIPKSTFELHDDGSGGVIIDSGTTITYIENSAFTSLKNEFIAQMNLPVDDSGTGGLDLCF 387
               + F     G G ++IDSGTT+T + ++ +  L++   + +         G L LC+
Sbjct: 303 QFTSTIF---GTGEGNIVIDSGTTLTLLPSNFYYELESVVASTIKAERVQDPDGILSLCY 362

Query: 388 NLPAGTNQVEVPKLTFHFKGADLELPGENYMIGDSKAGLLCLAIGSSRGMSIFGNLQQQN 447
                ++  +VP +T HFKG D++L   N  +  S+  + C A  ++  ++IFGNL Q N
Sbjct: 363 R---DSSSFKVPDITVHFKGGDVKLGNLNTFVAVSE-DVSCFAFAANEQLTIFGNLAQMN 422

Query: 448 FMVVHDLQEETLSFLPTQC 459
           F+V +D    T+SF  T C
Sbjct: 423 FLVGYDTVSGTVSFKKTDC 428

The following BLAST results are available for this feature:

BLAST of Chy8G146190 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q766C3	6.3e-119	51.89	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q766C2	2.3e-113	49.19	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q6XBF8	5.4e-70	36.04	Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1	[more]
Q7XV21	7.1e-70	35.12	Aspartyl protease 37 OS=Oryza sativa subsp. japonica OX=39947 GN=AP37 PE=3 SV=2	[more]
Q9LS40	5.6e-67	37.50	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]

BLAST of Chy8G146190 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KYT9	5.7e-256	97.83	Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G55468...	[more]
A0A5D3BTY9	1.1e-254	97.61	Aspartic proteinase nepenthesin-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A5A7TD10	2.4e-254	97.40	Aspartic proteinase nepenthesin-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2...	[more]
A0A1S3B573	2.4e-254	97.40	aspartic proteinase nepenthesin-1 OS=Cucumis melo OX=3656 GN=LOC103486136 PE=3 S...	[more]
A0A6J1HXS0	6.6e-212	83.92	aspartic proteinase nepenthesin-1 OS=Cucurbita maxima OX=3661 GN=LOC111467205 PE...	[more]

BLAST of Chy8G146190 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011653928.1	0.0	97.83	aspartic proteinase nepenthesin-1 [Cucumis sativus] >KGN54860.1 hypothetical pro...	[more]
TYK02448.1	0.0	97.61	aspartic proteinase nepenthesin-1 [Cucumis melo var. makuwa]	[more]
XP_008442220.1	0.0	97.40	PREDICTED: aspartic proteinase nepenthesin-1 [Cucumis melo] >KAA0041170.1 aspart...	[more]
XP_038883313.1	1.08e-289	89.52	aspartic proteinase nepenthesin-1 [Benincasa hispida]	[more]
XP_022967799.1	3.20e-268	85.71	aspartic proteinase nepenthesin-1 [Cucurbita maxima]	[more]

BLAST of Chy8G146190 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G03200.1	6.9e-153	61.18	Eukaryotic aspartyl protease family protein	[more]
AT5G33340.1	3.9e-71	36.04	Eukaryotic aspartyl protease family protein	[more]
AT1G25510.1	3.3e-70	35.81	Eukaryotic aspartyl protease family protein	[more]
AT3G18490.1	4.0e-68	37.50	Eukaryotic aspartyl protease family protein	[more]
AT1G64830.1	6.8e-68	35.08	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Aspartic peptidase A1 family	PRINTS	PR00792	PEPSIN	coord: 430..445 score: 26.0 coord: 117..137 score: 43.19 coord: 336..347 score: 38.11
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 111..277 e-value: 4.0E-52 score: 177.0
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 93..276 e-value: 2.6E-55 score: 189.4
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 281..460 e-value: 6.1E-54 score: 184.6
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 105..458
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 304..453 e-value: 6.6E-36 score: 123.5
None	No IPR available	PANTHER	PTHR47967:SF23	OS08G0469000 PROTEIN	coord: 27..460
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 27..460
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 336..347
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 111..454 score: 42.793736
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 110..458 e-value: 2.94548E-112 score: 329.609

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy8G146190.1	Chy8G146190.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004190	aspartic-type endopeptidase activity