Chy7G144250 (gene) Cucumber (hystrix) v1

Overview
NameChy7G144250
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionSNF1-related protein kinase regulatory subunit gamma-like PV42a
LocationchrH07: 20089367 .. 20091602 (+)
RNA-Seq ExpressionChy7G144250
SyntenyChy7G144250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGGCGACGGCAAAAAGAACCGAAACTCAGGCGGCGGTGGGAGAGAAGAAGGTGAAAGATCTGATGGGGGACAAGAGGCGGCTGGTGGAGGTGCCGTACACCGCTTCTCTTGCTCAAACAATGAACGTTCTAGTTGCGAATCATGTGGTGGCGGTTCCCGTGGCGGCGCCGCCTGGCCATTGGATTGGCGCAGGTGGTTCTATGATCATGGAATCTGATAAGCGGACTGGGGTTCTAAGAAAGCATTATATTGGTATGGTCACCATGCTTGATATCTTGGCTCATATTGCTGGCGATGATCACGATGGCGATGGCGGTCGTGATGATCTCATTGATCTCGATCGGAAGATGGCGGTTCCAGTATCTAATATTATTGGACATAATGTTGAAGGTTTGAGTCTCTGGACTTTGAATCCCAACACCAGGTAAGTGGATGGATAGATGGATGTTGATTTGAATTTATTGAAATATTATGTGAATTAAGAGGCTACATAATTCATGAAAAGAAGGTATTAATTCTCAACTTTTGCGTGTTTTTGGGGATGAATTTCAATTTGGTCTTTGATTTTTTTACCTTTTTAACTCTAGATTGTTTATTTATCTACAATTGAAAATATAGTCATCATGTTGAAAATTAAATTTAGCAAACATTGCTCAATACCAGAAAGAAAAAAAAAAGGAAGATATACTATTTTGCTATATATATATACAATCATTTTTAAAAAATATTATTAAACTTAATTATTGTCCCCTAAAGTTCTAACATTGCAATTACTTCTCTTTTGGTATTAAATCAAATCTTTTAACGCGAAAGATCAAAACAACTCCAAAAAGAAATGACAATGGTGTTATGGAAAGAAGCAACGTTTTTAAAACTAAAAATACAAATTTATATTGATTTGGGGTATAAGATAAATTGAATCAATGAAAATTTGAGAATATGAGAATAGTTGCGAGAAAGTAAAATTTATATGAAAGGATAAATTTGAGACTTGGGAAATGGATATGAAAGGTAGAAACGTGTAGTAAAAGCATAATTTAAGCTTTTGCAGTGTTAGAACCAATTAGAAATGATTCTAGTAGGAAATGATAAATAATTTGTTTGAATTTTCAACTGATATGATTTGTCTCGATCTGCTCGATCTTATAGCATATTAGATTGTATGGAGATATTCAGCAAGGGGATCCACCGAGCCCTGGTTCCTGTGGATGGGCAGGTTGAGGAGGCAGTAGGGGTGGAGCTGGTCGAGTCAGCGTCGAGTTACAGAATGCTGACCCAGATGGATGTGTTGAGGTTCCTAAGAGGAAAAGTGGCAGAAATTGAAGGGATTTTGAAGCAAAGTGTGAAGGAAATGGAAGGGATGGTGAATGAGAATGTTATGGCAATTACAGATAAAACCAGTGTCATTGAAGCTATCAAGTGCATGAAATCTAGTTTTCTCAATGCAGTTCCCATTGTTGGAAGTACTCAGCTCGGCGTCGACCAACAAAGCCACGCACAACTTTTTACCGTAAGTCAAATTTCTTTCCATTCTCGAGCCTTAGGACTTCACGAGGTTTGATATCATATTAGATAAACAGATTTTCTAACAAATAAAAGAAAGAAACATTCCACAAATTACAAGAGAGTAGAAAAGTTAGCGCAACAGTGGAAGAGTTTCTAATTTGACCCATGACTGACTTTTTTATTGAACTTGAGAGATCTCTTCATCTTTCTTGATCTAAGATCTCTCTTATCTCTTTTCAATGTTAGATCGTTAGCATGCGGCCTTAATCCGCAGAAGTCAAAAGATAGATCGATGTAAATTGTGTTATATTGTTATAGCCATGATCGTTATATATTTGCAACCCCGTTTTTTCTTTAAGCATTAACGAACGTGTGGATACGAGTTTCTATCAGGGAAGAGGAAAAAAACTAGTAGGGACATTCTCCGCAACGGACCTAAGGGGGTGCCATCTAGCGACACTGCAGTCATGGCTACACCAGACAGCGCTAGAGTTCACTGATTTAGTTAGAAAGAGTCCATTGTTGGAAGGGGCAGGGGTAGGGGTGAGAGAGCTGGTCACATGTCGGCCGGAAAGCTCGTTGGAGGAAGTGATGGAGAAGGTGTTGTCAAAACACGTGCACAGAATTTGGGTAACTGATGAACATGGTTTGCTGCTTGGCTTAATCTCCCTATCAGATATGATTAGAGTGATAAGGCTTTCACTTCTCTCTAAAATCCAAACCTAA

mRNA sequence

ATGCAGGCGACGGCAAAAAGAACCGAAACTCAGGCGGCGGTGGGAGAGAAGAAGGTGAAAGATCTGATGGGGGACAAGAGGCGGCTGGTGGAGGTGCCGTACACCGCTTCTCTTGCTCAAACAATGAACGTTCTAGTTGCGAATCATGTGGTGGCGGTTCCCGTGGCGGCGCCGCCTGGCCATTGGATTGGCGCAGGTGGTTCTATGATCATGGAATCTGATAAGCGGACTGGGGTTCTAAGAAAGCATTATATTGGTATGGTCACCATGCTTGATATCTTGGCTCATATTGCTGGCGATGATCACGATGGCGATGGCGGTCGTGATGATCTCATTGATCTCGATCGGAAGATGGCGGTTCCAGTATCTAATATTATTGGACATAATGTTGAAGGTTTGAGTCTCTGGACTTTGAATCCCAACACCAGCATATTAGATTGTATGGAGATATTCAGCAAGGGGATCCACCGAGCCCTGGTTCCTGTGGATGGGCAGGTTGAGGAGGCAGTAGGGGTGGAGCTGGTCGAGTCAGCGTCGAGTTACAGAATGCTGACCCAGATGGATGTGTTGAGGTTCCTAAGAGGAAAAGTGGCAGAAATTGAAGGGATTTTGAAGCAAAGTGTGAAGGAAATGGAAGGGATGGTGAATGAGAATGTTATGGCAATTACAGATAAAACCAGTGTCATTGAAGCTATCAAGTGCATGAAATCTAGTTTTCTCAATGCAGTTCCCATTGTTGGAAGTACTCAGCTCGGCGTCGACCAACAAAGCCACGCACAACTTTTTACCGGAAGAGGAAAAAAACTAGTAGGGACATTCTCCGCAACGGACCTAAGGGGGTGCCATCTAGCGACACTGCAGTCATGGCTACACCAGACAGCGCTAGAGTTCACTGATTTAGTTAGAAAGAGTCCATTGTTGGAAGGGGCAGGGGTAGGGGTGAGAGAGCTGGTCACATGTCGGCCGGAAAGCTCGTTGGAGGAAGTGATGGAGAAGGTGTTGTCAAAACACGTGCACAGAATTTGGGTAACTGATGAACATGGTTTGCTGCTTGGCTTAATCTCCCTATCAGATATGATTAGAGTGATAAGGCTTTCACTTCTCTCTAAAATCCAAACCTAA

Coding sequence (CDS)

ATGCAGGCGACGGCAAAAAGAACCGAAACTCAGGCGGCGGTGGGAGAGAAGAAGGTGAAAGATCTGATGGGGGACAAGAGGCGGCTGGTGGAGGTGCCGTACACCGCTTCTCTTGCTCAAACAATGAACGTTCTAGTTGCGAATCATGTGGTGGCGGTTCCCGTGGCGGCGCCGCCTGGCCATTGGATTGGCGCAGGTGGTTCTATGATCATGGAATCTGATAAGCGGACTGGGGTTCTAAGAAAGCATTATATTGGTATGGTCACCATGCTTGATATCTTGGCTCATATTGCTGGCGATGATCACGATGGCGATGGCGGTCGTGATGATCTCATTGATCTCGATCGGAAGATGGCGGTTCCAGTATCTAATATTATTGGACATAATGTTGAAGGTTTGAGTCTCTGGACTTTGAATCCCAACACCAGCATATTAGATTGTATGGAGATATTCAGCAAGGGGATCCACCGAGCCCTGGTTCCTGTGGATGGGCAGGTTGAGGAGGCAGTAGGGGTGGAGCTGGTCGAGTCAGCGTCGAGTTACAGAATGCTGACCCAGATGGATGTGTTGAGGTTCCTAAGAGGAAAAGTGGCAGAAATTGAAGGGATTTTGAAGCAAAGTGTGAAGGAAATGGAAGGGATGGTGAATGAGAATGTTATGGCAATTACAGATAAAACCAGTGTCATTGAAGCTATCAAGTGCATGAAATCTAGTTTTCTCAATGCAGTTCCCATTGTTGGAAGTACTCAGCTCGGCGTCGACCAACAAAGCCACGCACAACTTTTTACCGGAAGAGGAAAAAAACTAGTAGGGACATTCTCCGCAACGGACCTAAGGGGGTGCCATCTAGCGACACTGCAGTCATGGCTACACCAGACAGCGCTAGAGTTCACTGATTTAGTTAGAAAGAGTCCATTGTTGGAAGGGGCAGGGGTAGGGGTGAGAGAGCTGGTCACATGTCGGCCGGAAAGCTCGTTGGAGGAAGTGATGGAGAAGGTGTTGTCAAAACACGTGCACAGAATTTGGGTAACTGATGAACATGGTTTGCTGCTTGGCTTAATCTCCCTATCAGATATGATTAGAGTGATAAGGCTTTCACTTCTCTCTAAAATCCAAACCTAA

Protein sequence

MQATAKRTETQAAVGEKKVKDLMGDKRRLVEVPYTASLAQTMNVLVANHVVAVPVAAPPGHWIGAGGSMIMESDKRTGVLRKHYIGMVTMLDILAHIAGDDHDGDGGRDDLIDLDRKMAVPVSNIIGHNVEGLSLWTLNPNTSILDCMEIFSKGIHRALVPVDGQVEEAVGVELVESASSYRMLTQMDVLRFLRGKVAEIEGILKQSVKEMEGMVNENVMAITDKTSVIEAIKCMKSSFLNAVPIVGSTQLGVDQQSHAQLFTGRGKKLVGTFSATDLRGCHLATLQSWLHQTALEFTDLVRKSPLLEGAGVGVRELVTCRPESSLEEVMEKVLSKHVHRIWVTDEHGLLLGLISLSDMIRVIRLSLLSKIQT*
Homology
BLAST of Chy7G144250 vs. ExPASy Swiss-Prot
Match: Q9XI37 (SNF1-related protein kinase regulatory subunit gamma-like PV42a OS=Arabidopsis thaliana OX=3702 GN=PV42A PE=2 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 1.7e-106
Identity = 207/366 (56.56%), Postives = 270/366 (73.77%), Query Frame = 0

Query: 6   KRTETQAAVGEKKVKDLMGDKRRLVEVPYTASLAQTMNVLVANHVVAVPVAAPPGHWIGA 65
           K  E  + +     KDL    RRLVEVPYTA+L+  MN LVAN + A+PVAAPPGHWIGA
Sbjct: 5   KSKEDHSRLINVTAKDLTVRNRRLVEVPYTATLSHAMNTLVANSISALPVAAPPGHWIGA 64

Query: 66  GGSMIMESDKRTGVLRKHYIGMVTMLDILAHIAGDDHDGDGGRDDLIDLDRKMAVPVSNI 125
           GGSMIMESDK+TGV+RKHYIG++TMLDILAHIAG+D        +L DLDRKM+  VS+I
Sbjct: 65  GGSMIMESDKQTGVVRKHYIGILTMLDILAHIAGED-------SNLSDLDRKMSSQVSSI 124

Query: 126 IGHNVEGLSLWTLNPNTSILDCMEIFSKGIHRALVPVDGQVEE---AVGVELVESASSYR 185
           IGH +EGLSLWTLNPNTS+L+CME+FSKGIHRALVPV+  +E      GVEL+ESAS+Y+
Sbjct: 125 IGHCLEGLSLWTLNPNTSVLECMEVFSKGIHRALVPVESSIESNNTIAGVELIESASAYK 184

Query: 186 MLTQMDVLRFLRG-KVAEIEGILKQSVKEMEGMVNENVMAITDKTSVIEAIKCMKSSFLN 245
           MLTQMD+LRFL+     +++ +L +S+ ++ G VN++V AIT++T+V  AI  MK + LN
Sbjct: 185 MLTQMDLLRFLKDHHFDDLKTVLSRSISDL-GAVNDSVYAITERTTVSNAINVMKGALLN 244

Query: 246 AVPIVGSTQLGVDQQSHAQLFTGRGKKLVGTFSATDLRGCHLATLQSWLHQTALEFTDLV 305
           AVPIV +  +   Q+ H QL  GR +K++GTFSATDL+GC L  LQ+WL  TALEFT   
Sbjct: 245 AVPIVHAPDIA--QEDHLQLVNGRHRKVIGTFSATDLKGCRLPELQTWLPLTALEFT--- 304

Query: 306 RKSPLLEGAGVGVRELVTCRPESSLEEVMEKVLSKHVHRIWVTDEHGLLLGLISLSDMIR 365
                 E      RE+V+C  ES++EE +EKV+++ VHR+WV D+ GLL G++SL+D+IR
Sbjct: 305 ------EKTSGKEREVVSCGVESTMEEAIEKVVTRGVHRVWVMDQQGLLQGVVSLTDIIR 351

Query: 366 VIRLSL 368
            +R +L
Sbjct: 365 SLRSTL 351

BLAST of Chy7G144250 vs. ExPASy Swiss-Prot
Match: Q8GXI9 (SNF1-related protein kinase regulatory subunit gamma-like PV42b OS=Arabidopsis thaliana OX=3702 GN=PV42B PE=2 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 2.8e-101
Identity = 196/353 (55.52%), Postives = 256/353 (72.52%), Query Frame = 0

Query: 18  KVKDLMGDKRRLVEVPYTASLAQTMNVLVANHVVAVPVAAPPGHWIGAGGSMIMESDKRT 77
           KVKDLM DKRRLVEVP  A+L   +N +VAN V AVPVAA PG W+GAGGSMI+E DK++
Sbjct: 11  KVKDLMIDKRRLVEVPDNATLGDALNTMVANRVRAVPVAAKPGQWLGAGGSMIVELDKQS 70

Query: 78  GVLRKHYIGMVTMLDILAHIAGDDHDGDGGRDDLIDLDRKMAVPVSNIIGHNVEGLSLWT 137
           G  RK YIGMVTMLD++AHIAGD  DG+ G      LD+KMA PVS+IIGH  EGLSLW+
Sbjct: 71  GSARKQYIGMVTMLDVVAHIAGD--DGESG------LDKKMAAPVSSIIGHCPEGLSLWS 130

Query: 138 LNPNTSILDCMEIFSKGIHRALVPVDGQVEEAVGVELVESASSYRMLTQMDVLRFLRGKV 197
           LNPNTSI+DCME+ SKGIHR LVP+D   E   G ELVESAS+Y ML+QMD++ F   + 
Sbjct: 131 LNPNTSIMDCMEMLSKGIHRVLVPLDSNTENITGPELVESASAYAMLSQMDLISFFFDQS 190

Query: 198 AEIEGILKQSVKEMEGMVNENVMAITDKTSVIEAIKCMKSSFLNAVPIVGSTQLGVDQQS 257
           +++ GIL  +V ++   ++  V+A+T +  V +AI+CM  + LNAVPIV ++  G D   
Sbjct: 191 SQLHGILSHTVTDLSA-IHNTVLALTSQARVKDAIQCMSIAMLNAVPIVEASGEGED--- 250

Query: 258 HAQLFTGRGKKLVGTFSATDLRGCHLATLQSWLHQTALEFTDLVRKSPLLEGA-GVGVRE 317
           H QL  G+ +++VGTFSA+DL+GCHLATL+SWL   ALEF + + ++ L   A     RE
Sbjct: 251 HKQLVDGKNRRVVGTFSASDLKGCHLATLRSWLPLNALEFVEKIPRTLLFTAATSTPGRE 310

Query: 318 LVTCRPESSLEEVMEKVLSKHVHRIWVTDEHGLLLGLISLSDMIRVIRLSLLS 370
           LVTC   S+L +V+  V +K VHR+WV D++G L GL+SL+D+I V+R +LLS
Sbjct: 311 LVTCHVTSTLAQVIHMVTTKRVHRVWVVDQNGGLQGLVSLTDIIAVVRSALLS 351

BLAST of Chy7G144250 vs. ExPASy Swiss-Prot
Match: Q9UGI9 (5'-AMP-activated protein kinase subunit gamma-3 OS=Homo sapiens OX=9606 GN=PRKAG3 PE=1 SV=3)

HSP 1 Score: 62.8 bits (151), Expect = 9.7e-09
Identity = 61/237 (25.74%), Postives = 110/237 (46.41%), Query Frame = 0

Query: 135 LWTLNPNTSILDCMEIFSKG-IHR--ALVPVDGQVEEAVGVELVESASSYRMLTQMDVLR 194
           L +++PN S+ + +    K  IHR   L PV G V                +LT   +L+
Sbjct: 284 LVSISPNDSLFEAVYTLIKNRIHRLPVLDPVSGNV--------------LHILTHKRLLK 343

Query: 195 FLR--GKVAEIEGILKQSVKEMEGMVNENVMAITDKTSVIEAIKCMKSSFLNAVPIVGST 254
           FL   G +      L ++++++       +    D   V+E             PI+ + 
Sbjct: 344 FLHIFGSLLPRPSFLYRTIQDL------GIGTFRDLAVVLE-----------TAPILTAL 403

Query: 255 QLGVDQQSHAQLFTGRGKKLVGTFSATDLRGCHLATLQSWLHQTALEFTDLVRKSPLLEG 314
            + VD++  A        ++VG +S  D+   HLA  Q++ H        L +++  LEG
Sbjct: 404 DIFVDRRVSALPVVNECGQVVGLYSRFDV--IHLAAQQTYNHLDMSVGEALRQRTLCLEG 463

Query: 315 AGVGVRELVTCRPESSLEEVMEKVLSKHVHRIWVTDEHGLLLGLISLSDMIRVIRLS 367
                  +++C+P  SL EV++++  + VHR+ + DE   LLG++SLSD+++ + LS
Sbjct: 464 -------VLSCQPHESLGEVIDRIAREQVHRLVLVDETQHLLGVVSLSDILQALVLS 480

BLAST of Chy7G144250 vs. ExPASy Swiss-Prot
Match: Q9P869 (5'-AMP-activated protein kinase subunit gamma OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) OX=284590 GN=SNF4 PE=3 SV=2)

HSP 1 Score: 62.8 bits (151), Expect = 9.7e-09
Identity = 72/283 (25.44%), Postives = 133/283 (47.00%), Query Frame = 0

Query: 84  YIGMVTMLDILAHIAGDDHDGDGGRDDLID-LDRKMAVPVSNIIGHNVEGLSLWTLNPNT 143
           + G++T  D +  I    H+ D  + +L+D L       +   IG  ++     +++P  
Sbjct: 80  FAGLLTSSDFINVIQYYFHNPD--KFELVDKLQLNGLKDIERAIG--IQPYDTRSIHPFR 139

Query: 144 SILD-CMEIFSKGIHRALVPVDGQVEEAVGVELVESASSYRMLTQMDVLRFLRGKVAEIE 203
            + + C+++      R  +P+  Q EE    E+V S     +LTQ  +L+F+     EI 
Sbjct: 140 PLYEACVKMIESRSRR--IPLIDQDEETQR-EIVVS-----VLTQYRILKFVALNCKEIR 199

Query: 204 GILKQSVKEMEGMVNENVMAITDKTSVIEAIKCMK-SSFLNAVPIVGSTQLGVDQQSHAQ 263
             LK+ ++E++ +   N+M+    T VI+ I+ +  +  +++VPI       VD+Q    
Sbjct: 200 -YLKRPLRELDIISTNNIMSCQMSTPVIDVIQLLTLAGGVSSVPI-------VDEQG--- 259

Query: 264 LFTGRGKKLVGTFSATDLRGCHLATLQSWLHQTALEFTDLVRKSPLLEGAGVGVRELVTC 323
                  KLV  + A D+ G     + + L  +  E   L+R+S   EG       + TC
Sbjct: 260 -------KLVNVYEAVDVLGLIKGGIYNDLSLSVGE--ALMRRSDDFEG-------VFTC 319

Query: 324 RPESSLEEVMEKVLSKHVHRIWVTDEHGLLLGLISLSDMIRVI 364
                L  +++ V    VHR +V D +G L G+++LSD+++ I
Sbjct: 320 TENDKLSSILDTVRKSRVHRFFVVDSNGFLTGVLTLSDILKYI 323

BLAST of Chy7G144250 vs. ExPASy Swiss-Prot
Match: Q2LL38 (5'-AMP-activated protein kinase subunit gamma-3 OS=Bos taurus OX=9913 GN=PRKAG3 PE=2 SV=2)

HSP 1 Score: 61.6 bits (148), Expect = 2.2e-08
Identity = 39/127 (30.71%), Postives = 70/127 (55.12%), Query Frame = 0

Query: 240 LNAVPIVGSTQLGVDQQSHAQLFTGRGKKLVGTFSATDLRGCHLATLQSWLHQTALEFTD 299
           L   PI+ +  + VD++  A        ++VG +S  D+   HLA  Q++ H        
Sbjct: 371 LETAPILTALDIFVDRRVSALPVINEAGQVVGLYSRFDV--IHLAAQQTYNHLDISVGEA 430

Query: 300 LVRKSPLLEGAGVGVRELVTCRPESSLEEVMEKVLSKHVHRIWVTDEHGLLLGLISLSDM 359
           L R++  LEG       +++C+P  +L EV++++  + VHR+ + DE   LLG++SLSD+
Sbjct: 431 LRRRTLCLEG-------VLSCQPHETLGEVIDRIAREQVHRLVLVDETQHLLGVVSLSDI 488

Query: 360 IRVIRLS 367
           ++ + LS
Sbjct: 491 LQALVLS 488

BLAST of Chy7G144250 vs. ExPASy TrEMBL
Match: A0A0A0KYI0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G006180 PE=4 SV=1)

HSP 1 Score: 721.1 bits (1860), Expect = 2.4e-204
Identity = 369/373 (98.93%), Postives = 372/373 (99.73%), Query Frame = 0

Query: 1   MQATAKRTETQAAVGEKKVKDLMGDKRRLVEVPYTASLAQTMNVLVANHVVAVPVAAPPG 60
           MQATAKRTETQA+VGEKKVKDLMGDK+RLVEVPYTASLAQTMNVLVANHVVAVPVAAPPG
Sbjct: 1   MQATAKRTETQASVGEKKVKDLMGDKKRLVEVPYTASLAQTMNVLVANHVVAVPVAAPPG 60

Query: 61  HWIGAGGSMIMESDKRTGVLRKHYIGMVTMLDILAHIAGDDHDGDGGRDDLIDLDRKMAV 120
           HWIGAGGSMIMESDKRTGVLRKHYIGMVTMLDILAHIAGDDHDGDGGRDDLIDLDRKMAV
Sbjct: 61  HWIGAGGSMIMESDKRTGVLRKHYIGMVTMLDILAHIAGDDHDGDGGRDDLIDLDRKMAV 120

Query: 121 PVSNIIGHNVEGLSLWTLNPNTSILDCMEIFSKGIHRALVPVDGQVEEAVGVELVESASS 180
           PVSNIIGHNVEGLSLWTLNPNTSILDCMEIFSKGIHRALVPVDGQVEEAVGVELVESASS
Sbjct: 121 PVSNIIGHNVEGLSLWTLNPNTSILDCMEIFSKGIHRALVPVDGQVEEAVGVELVESASS 180

Query: 181 YRMLTQMDVLRFLRGKVAEIEGILKQSVKEMEGMVNENVMAITDKTSVIEAIKCMKSSFL 240
           YRMLTQMDVLRFLRGKV EIEGIL+QSVKEMEGMVNENVMAITDKTSVIEAIKCMKSSFL
Sbjct: 181 YRMLTQMDVLRFLRGKVVEIEGILRQSVKEMEGMVNENVMAITDKTSVIEAIKCMKSSFL 240

Query: 241 NAVPIVGSTQLGVDQQSHAQLFTGRGKKLVGTFSATDLRGCHLATLQSWLHQTALEFTDL 300
           NAVPIVGSTQLGVDQQSHAQLFTGRGKKLVGTFSATDLRGCHLATLQSWLHQTALEFTDL
Sbjct: 241 NAVPIVGSTQLGVDQQSHAQLFTGRGKKLVGTFSATDLRGCHLATLQSWLHQTALEFTDL 300

Query: 301 VRKSPLLEGAGVGVRELVTCRPESSLEEVMEKVLSKHVHRIWVTDEHGLLLGLISLSDMI 360
           VRKSPLLEGAGVGVRELVTCRPESSLEEVMEKVLSKHVHRIWVTDEHGLLLGLISLSDMI
Sbjct: 301 VRKSPLLEGAGVGVRELVTCRPESSLEEVMEKVLSKHVHRIWVTDEHGLLLGLISLSDMI 360

Query: 361 RVIRLSLLSKIQT 374
           RVIRLSLLSKIQT
Sbjct: 361 RVIRLSLLSKIQT 373

BLAST of Chy7G144250 vs. ExPASy TrEMBL
Match: A0A5A7TQS5 (SNF1-related protein kinase regulatory subunit gamma-like PV42a OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001890 PE=4 SV=1)

HSP 1 Score: 714.5 bits (1843), Expect = 2.3e-202
Identity = 368/374 (98.40%), Postives = 372/374 (99.47%), Query Frame = 0

Query: 1   MQATAKR-TETQAAVGEKKVKDLMGDKRRLVEVPYTASLAQTMNVLVANHVVAVPVAAPP 60
           MQATAKR TETQAAVGEKKVKDLMGDKRRLVEVPYTASLAQTMNVLVANHVVAVPVAAPP
Sbjct: 1   MQATAKRITETQAAVGEKKVKDLMGDKRRLVEVPYTASLAQTMNVLVANHVVAVPVAAPP 60

Query: 61  GHWIGAGGSMIMESDKRTGVLRKHYIGMVTMLDILAHIAGDDHDGDGGRDDLIDLDRKMA 120
           GHWIGAGGSMIMESDKRTGVLRKHYIGMVTMLDILAHIAGDDHDGDGGRDDLIDL+RKMA
Sbjct: 61  GHWIGAGGSMIMESDKRTGVLRKHYIGMVTMLDILAHIAGDDHDGDGGRDDLIDLERKMA 120

Query: 121 VPVSNIIGHNVEGLSLWTLNPNTSILDCMEIFSKGIHRALVPVDGQVEEAVGVELVESAS 180
           VPVSNIIGHNVEGLSLWTLNPNTSILDCMEIFSKGIHRALVPVDGQVEEAVGVELVESAS
Sbjct: 121 VPVSNIIGHNVEGLSLWTLNPNTSILDCMEIFSKGIHRALVPVDGQVEEAVGVELVESAS 180

Query: 181 SYRMLTQMDVLRFLRGKVAEIEGILKQSVKEMEGMVNENVMAITDKTSVIEAIKCMKSSF 240
           SYRMLTQMDVLRFLRGKVAEIEGIL+ SVKEMEGM+NENVMAITDKTSVIEAIKCMKSSF
Sbjct: 181 SYRMLTQMDVLRFLRGKVAEIEGILRLSVKEMEGMMNENVMAITDKTSVIEAIKCMKSSF 240

Query: 241 LNAVPIVGSTQLGVDQQSHAQLFTGRGKKLVGTFSATDLRGCHLATLQSWLHQTALEFTD 300
           LNAVPIVGSTQLGVDQQSHAQLFTGRGKKLVGTFSATDLRGCHLATLQSWLHQTALEFTD
Sbjct: 241 LNAVPIVGSTQLGVDQQSHAQLFTGRGKKLVGTFSATDLRGCHLATLQSWLHQTALEFTD 300

Query: 301 LVRKSPLLEGAGVGVRELVTCRPESSLEEVMEKVLSKHVHRIWVTDEHGLLLGLISLSDM 360
           LVRKSPLLEGAGVGVRELVTCRPESSLEEVMEKVLSKHVHR+WVTDEHGLLLGLISLSDM
Sbjct: 301 LVRKSPLLEGAGVGVRELVTCRPESSLEEVMEKVLSKHVHRVWVTDEHGLLLGLISLSDM 360

Query: 361 IRVIRLSLLSKIQT 374
           IRVIRLSLLSKIQT
Sbjct: 361 IRVIRLSLLSKIQT 374

BLAST of Chy7G144250 vs. ExPASy TrEMBL
Match: A0A1S3BY75 (SNF1-related protein kinase regulatory subunit gamma-like PV42a OS=Cucumis melo OX=3656 GN=LOC103494687 PE=4 SV=1)

HSP 1 Score: 714.5 bits (1843), Expect = 2.3e-202
Identity = 368/374 (98.40%), Postives = 372/374 (99.47%), Query Frame = 0

Query: 1   MQATAKR-TETQAAVGEKKVKDLMGDKRRLVEVPYTASLAQTMNVLVANHVVAVPVAAPP 60
           MQATAKR TETQAAVGEKKVKDLMGDKRRLVEVPYTASLAQTMNVLVANHVVAVPVAAPP
Sbjct: 1   MQATAKRITETQAAVGEKKVKDLMGDKRRLVEVPYTASLAQTMNVLVANHVVAVPVAAPP 60

Query: 61  GHWIGAGGSMIMESDKRTGVLRKHYIGMVTMLDILAHIAGDDHDGDGGRDDLIDLDRKMA 120
           GHWIGAGGSMIMESDKRTGVLRKHYIGMVTMLDILAHIAGDDHDGDGGRDDLIDL+RKMA
Sbjct: 61  GHWIGAGGSMIMESDKRTGVLRKHYIGMVTMLDILAHIAGDDHDGDGGRDDLIDLERKMA 120

Query: 121 VPVSNIIGHNVEGLSLWTLNPNTSILDCMEIFSKGIHRALVPVDGQVEEAVGVELVESAS 180
           VPVSNIIGHNVEGLSLWTLNPNTSILDCMEIFSKGIHRALVPVDGQVEEAVGVELVESAS
Sbjct: 121 VPVSNIIGHNVEGLSLWTLNPNTSILDCMEIFSKGIHRALVPVDGQVEEAVGVELVESAS 180

Query: 181 SYRMLTQMDVLRFLRGKVAEIEGILKQSVKEMEGMVNENVMAITDKTSVIEAIKCMKSSF 240
           SYRMLTQMDVLRFLRGKVAEIEGIL+ SVKEMEGM+NENVMAITDKTSVIEAIKCMKSSF
Sbjct: 181 SYRMLTQMDVLRFLRGKVAEIEGILRLSVKEMEGMMNENVMAITDKTSVIEAIKCMKSSF 240

Query: 241 LNAVPIVGSTQLGVDQQSHAQLFTGRGKKLVGTFSATDLRGCHLATLQSWLHQTALEFTD 300
           LNAVPIVGSTQLGVDQQSHAQLFTGRGKKLVGTFSATDLRGCHLATLQSWLHQTALEFTD
Sbjct: 241 LNAVPIVGSTQLGVDQQSHAQLFTGRGKKLVGTFSATDLRGCHLATLQSWLHQTALEFTD 300

Query: 301 LVRKSPLLEGAGVGVRELVTCRPESSLEEVMEKVLSKHVHRIWVTDEHGLLLGLISLSDM 360
           LVRKSPLLEGAGVGVRELVTCRPESSLEEVMEKVLSKHVHR+WVTDEHGLLLGLISLSDM
Sbjct: 301 LVRKSPLLEGAGVGVRELVTCRPESSLEEVMEKVLSKHVHRVWVTDEHGLLLGLISLSDM 360

Query: 361 IRVIRLSLLSKIQT 374
           IRVIRLSLLSKIQT
Sbjct: 361 IRVIRLSLLSKIQT 374

BLAST of Chy7G144250 vs. ExPASy TrEMBL
Match: A0A6J1ITT2 (SNF1-related protein kinase regulatory subunit gamma-like PV42a OS=Cucurbita maxima OX=3661 GN=LOC111479295 PE=4 SV=1)

HSP 1 Score: 560.8 bits (1444), Expect = 4.2e-156
Identity = 297/374 (79.41%), Postives = 328/374 (87.70%), Query Frame = 0

Query: 2   QATAKRTETQAAVGEKKVKDLMGDKRRLVEVPYTASLAQTMNVLVANHVVAVPVAAPPGH 61
           Q TAK+ +TQA V +KKVKDLM D RRLVEVPYTASLA TMNVLVAN VVAVPVAAPPGH
Sbjct: 3   QPTAKKPDTQAVV-DKKVKDLMRDWRRLVEVPYTASLAHTMNVLVANCVVAVPVAAPPGH 62

Query: 62  WIGAGGSMIMESDKRTGVLRKHYIGMVTMLDILAHIAG--DDHDGDGGRDDLIDLDRKMA 121
           WIGAGGSMIME+DK +GV+RKHYIGMVTMLDILAHIAG  D HD  G   DL+DLDRKMA
Sbjct: 63  WIGAGGSMIMEADKSSGVVRKHYIGMVTMLDILAHIAGSEDVHDSSG---DLLDLDRKMA 122

Query: 122 VPVSNIIGHNVEGLSLWTLNPNTSILDCMEIFSKGIHRALVPVDGQVEEAVGVELVESAS 181
           VPVS+IIGH+VEGLSLWTLNPNTSILDCMEIFSKGIHRALVP+DGQVEEA+GV+LVESA+
Sbjct: 123 VPVSSIIGHSVEGLSLWTLNPNTSILDCMEIFSKGIHRALVPIDGQVEEAMGVKLVESAT 182

Query: 182 SYRMLTQMDVLRFLRGKVAEIEGILKQSVKEMEGMVNENVMAITDKTSVIEAIKCMKSSF 241
           SYRM+TQMDVLRFLR K  EI+ IL+QSVKEMEG +NENVMAITDKT +IEAIKCMKSSF
Sbjct: 183 SYRMVTQMDVLRFLREKAPEIKEILRQSVKEMEG-INENVMAITDKTKLIEAIKCMKSSF 242

Query: 242 LNAVPIVGSTQLGVDQQSHAQLFTGRGKKLVGTFSATDLRGCHLATLQSWLHQTALEFTD 301
           LNAVPIVG   +      H QLFTGRG KLVGTFSATDLRGC LATLQ+WLH++ L+FTD
Sbjct: 243 LNAVPIVGGDHV------HTQLFTGRGMKLVGTFSATDLRGCQLATLQTWLHRSVLDFTD 302

Query: 302 LVRKSPLLEGAGVGVRELVTCRPESSLEEVMEKVLSKHVHRIWVTDEHGLLLGLISLSDM 361
            VR S L EG+    RELVTC+PESSLEEVMEKV+SKHVHRIWV DEHGLLLG++SLSDM
Sbjct: 303 AVRNSSLFEGSVAFGRELVTCQPESSLEEVMEKVVSKHVHRIWVIDEHGLLLGVVSLSDM 362

Query: 362 IRVIRLSLLSKIQT 374
           IRV+R SLLS++QT
Sbjct: 363 IRVVRHSLLSQLQT 365

BLAST of Chy7G144250 vs. ExPASy TrEMBL
Match: A0A6J1GXB5 (SNF1-related protein kinase regulatory subunit gamma-like PV42a OS=Cucurbita moschata OX=3662 GN=LOC111457684 PE=4 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 2.1e-155
Identity = 295/374 (78.88%), Postives = 325/374 (86.90%), Query Frame = 0

Query: 2   QATAKRTETQAAVGEKKVKDLMGDKRRLVEVPYTASLAQTMNVLVANHVVAVPVAAPPGH 61
           Q TAK+ +T  AV +KKVKDLM D RRLVEVPYTASLA TMNVLVAN VVAVPVAAPPGH
Sbjct: 3   QPTAKKPDTIQAVVDKKVKDLMRDWRRLVEVPYTASLAHTMNVLVANCVVAVPVAAPPGH 62

Query: 62  WIGAGGSMIMESDKRTGVLRKHYIGMVTMLDILAHIAG--DDHDGDGGRDDLIDLDRKMA 121
           WIGAGGSMIME+DK TGV+RKHYIGMVTMLDILAHIAG  D HD  G   DL+DLDRKMA
Sbjct: 63  WIGAGGSMIMEADKSTGVVRKHYIGMVTMLDILAHIAGSEDAHDSSG---DLLDLDRKMA 122

Query: 122 VPVSNIIGHNVEGLSLWTLNPNTSILDCMEIFSKGIHRALVPVDGQVEEAVGVELVESAS 181
           VP+S+IIGH+VEGLSLWTLNPNTSILDCMEIFSKGIHRALVPVDGQVEEAVGV+LVESA+
Sbjct: 123 VPISSIIGHSVEGLSLWTLNPNTSILDCMEIFSKGIHRALVPVDGQVEEAVGVKLVESAT 182

Query: 182 SYRMLTQMDVLRFLRGKVAEIEGILKQSVKEMEGMVNENVMAITDKTSVIEAIKCMKSSF 241
           SY M+TQMDVLRFLR +  EI+ IL+QSVKEMEG +NENVMAITD+T +IEAIKCMKSSF
Sbjct: 183 SYMMVTQMDVLRFLRERTPEIKAILRQSVKEMEG-INENVMAITDQTKLIEAIKCMKSSF 242

Query: 242 LNAVPIVGSTQLGVDQQSHAQLFTGRGKKLVGTFSATDLRGCHLATLQSWLHQTALEFTD 301
           LNAVPIVG   L      H QL TGRG+KLVGTFSATDLRGC LATLQ+WLH++ L+FTD
Sbjct: 243 LNAVPIVGGDHL------HTQLITGRGRKLVGTFSATDLRGCQLATLQTWLHRSVLDFTD 302

Query: 302 LVRKSPLLEGAGVGVRELVTCRPESSLEEVMEKVLSKHVHRIWVTDEHGLLLGLISLSDM 361
            VR S L EG+    RELVTCRPESSLEEVMEKV+SKHVHRIWV DEHGLLLG++SLSDM
Sbjct: 303 AVRNSLLFEGSVAVGRELVTCRPESSLEEVMEKVVSKHVHRIWVIDEHGLLLGVVSLSDM 362

Query: 362 IRVIRLSLLSKIQT 374
           IRV+R SLLS++ T
Sbjct: 363 IRVVRHSLLSQLHT 366

BLAST of Chy7G144250 vs. NCBI nr
Match: XP_004152338.2 (SNF1-related protein kinase regulatory subunit gamma-like PV42a [Cucumis sativus] >KAE8649061.1 hypothetical protein Csa_015412 [Cucumis sativus])

HSP 1 Score: 719 bits (1856), Expect = 3.55e-260
Identity = 369/373 (98.93%), Postives = 372/373 (99.73%), Query Frame = 0

Query: 1   MQATAKRTETQAAVGEKKVKDLMGDKRRLVEVPYTASLAQTMNVLVANHVVAVPVAAPPG 60
           MQATAKRTETQA+VGEKKVKDLMGDK+RLVEVPYTASLAQTMNVLVANHVVAVPVAAPPG
Sbjct: 52  MQATAKRTETQASVGEKKVKDLMGDKKRLVEVPYTASLAQTMNVLVANHVVAVPVAAPPG 111

Query: 61  HWIGAGGSMIMESDKRTGVLRKHYIGMVTMLDILAHIAGDDHDGDGGRDDLIDLDRKMAV 120
           HWIGAGGSMIMESDKRTGVLRKHYIGMVTMLDILAHIAGDDHDGDGGRDDLIDLDRKMAV
Sbjct: 112 HWIGAGGSMIMESDKRTGVLRKHYIGMVTMLDILAHIAGDDHDGDGGRDDLIDLDRKMAV 171

Query: 121 PVSNIIGHNVEGLSLWTLNPNTSILDCMEIFSKGIHRALVPVDGQVEEAVGVELVESASS 180
           PVSNIIGHNVEGLSLWTLNPNTSILDCMEIFSKGIHRALVPVDGQVEEAVGVELVESASS
Sbjct: 172 PVSNIIGHNVEGLSLWTLNPNTSILDCMEIFSKGIHRALVPVDGQVEEAVGVELVESASS 231

Query: 181 YRMLTQMDVLRFLRGKVAEIEGILKQSVKEMEGMVNENVMAITDKTSVIEAIKCMKSSFL 240
           YRMLTQMDVLRFLRGKV EIEGIL+QSVKEMEGMVNENVMAITDKTSVIEAIKCMKSSFL
Sbjct: 232 YRMLTQMDVLRFLRGKVVEIEGILRQSVKEMEGMVNENVMAITDKTSVIEAIKCMKSSFL 291

Query: 241 NAVPIVGSTQLGVDQQSHAQLFTGRGKKLVGTFSATDLRGCHLATLQSWLHQTALEFTDL 300
           NAVPIVGSTQLGVDQQSHAQLFTGRGKKLVGTFSATDLRGCHLATLQSWLHQTALEFTDL
Sbjct: 292 NAVPIVGSTQLGVDQQSHAQLFTGRGKKLVGTFSATDLRGCHLATLQSWLHQTALEFTDL 351

Query: 301 VRKSPLLEGAGVGVRELVTCRPESSLEEVMEKVLSKHVHRIWVTDEHGLLLGLISLSDMI 360
           VRKSPLLEGAGVGVRELVTCRPESSLEEVMEKVLSKHVHRIWVTDEHGLLLGLISLSDMI
Sbjct: 352 VRKSPLLEGAGVGVRELVTCRPESSLEEVMEKVLSKHVHRIWVTDEHGLLLGLISLSDMI 411

Query: 361 RVIRLSLLSKIQT 373
           RVIRLSLLSKIQT
Sbjct: 412 RVIRLSLLSKIQT 424

BLAST of Chy7G144250 vs. NCBI nr
Match: XP_008454212.1 (PREDICTED: SNF1-related protein kinase regulatory subunit gamma-like PV42a [Cucumis melo] >KAA0044456.1 SNF1-related protein kinase regulatory subunit gamma-like PV42a [Cucumis melo var. makuwa] >TYK29583.1 SNF1-related protein kinase regulatory subunit gamma-like PV42a [Cucumis melo var. makuwa])

HSP 1 Score: 712 bits (1839), Expect = 2.12e-258
Identity = 368/374 (98.40%), Postives = 372/374 (99.47%), Query Frame = 0

Query: 1   MQATAKR-TETQAAVGEKKVKDLMGDKRRLVEVPYTASLAQTMNVLVANHVVAVPVAAPP 60
           MQATAKR TETQAAVGEKKVKDLMGDKRRLVEVPYTASLAQTMNVLVANHVVAVPVAAPP
Sbjct: 1   MQATAKRITETQAAVGEKKVKDLMGDKRRLVEVPYTASLAQTMNVLVANHVVAVPVAAPP 60

Query: 61  GHWIGAGGSMIMESDKRTGVLRKHYIGMVTMLDILAHIAGDDHDGDGGRDDLIDLDRKMA 120
           GHWIGAGGSMIMESDKRTGVLRKHYIGMVTMLDILAHIAGDDHDGDGGRDDLIDL+RKMA
Sbjct: 61  GHWIGAGGSMIMESDKRTGVLRKHYIGMVTMLDILAHIAGDDHDGDGGRDDLIDLERKMA 120

Query: 121 VPVSNIIGHNVEGLSLWTLNPNTSILDCMEIFSKGIHRALVPVDGQVEEAVGVELVESAS 180
           VPVSNIIGHNVEGLSLWTLNPNTSILDCMEIFSKGIHRALVPVDGQVEEAVGVELVESAS
Sbjct: 121 VPVSNIIGHNVEGLSLWTLNPNTSILDCMEIFSKGIHRALVPVDGQVEEAVGVELVESAS 180

Query: 181 SYRMLTQMDVLRFLRGKVAEIEGILKQSVKEMEGMVNENVMAITDKTSVIEAIKCMKSSF 240
           SYRMLTQMDVLRFLRGKVAEIEGIL+ SVKEMEGM+NENVMAITDKTSVIEAIKCMKSSF
Sbjct: 181 SYRMLTQMDVLRFLRGKVAEIEGILRLSVKEMEGMMNENVMAITDKTSVIEAIKCMKSSF 240

Query: 241 LNAVPIVGSTQLGVDQQSHAQLFTGRGKKLVGTFSATDLRGCHLATLQSWLHQTALEFTD 300
           LNAVPIVGSTQLGVDQQSHAQLFTGRGKKLVGTFSATDLRGCHLATLQSWLHQTALEFTD
Sbjct: 241 LNAVPIVGSTQLGVDQQSHAQLFTGRGKKLVGTFSATDLRGCHLATLQSWLHQTALEFTD 300

Query: 301 LVRKSPLLEGAGVGVRELVTCRPESSLEEVMEKVLSKHVHRIWVTDEHGLLLGLISLSDM 360
           LVRKSPLLEGAGVGVRELVTCRPESSLEEVMEKVLSKHVHR+WVTDEHGLLLGLISLSDM
Sbjct: 301 LVRKSPLLEGAGVGVRELVTCRPESSLEEVMEKVLSKHVHRVWVTDEHGLLLGLISLSDM 360

Query: 361 IRVIRLSLLSKIQT 373
           IRVIRLSLLSKIQT
Sbjct: 361 IRVIRLSLLSKIQT 374

BLAST of Chy7G144250 vs. NCBI nr
Match: XP_038877427.1 (SNF1-related protein kinase regulatory subunit gamma-like PV42a isoform X2 [Benincasa hispida])

HSP 1 Score: 639 bits (1647), Expect = 3.96e-229
Identity = 336/377 (89.12%), Postives = 351/377 (93.10%), Query Frame = 0

Query: 1   MQATAKRTETQAAVGEKKVKDLMGDKRRLVEVPYTASLAQTMNVLVANHVVAVPVAAPPG 60
           MQ TAKRTET+A V EKKVKDLMG+KRRLVEVPYTASLAQTMNVLVAN VVAVPVAAPPG
Sbjct: 1   MQRTAKRTETEAVV-EKKVKDLMGEKRRLVEVPYTASLAQTMNVLVANRVVAVPVAAPPG 60

Query: 61  HWIGAGGSMIMESDKRTGVLRKHYIGMVTMLDILAHIAGDD--HDGDGGRDDLIDLDRKM 120
           HWIGAGGSMIMESDKRTGVLRKHYIGMVTMLDILAHIAGDD  HD DGG D L+D+DRKM
Sbjct: 61  HWIGAGGSMIMESDKRTGVLRKHYIGMVTMLDILAHIAGDDQDHDHDGGHD-LLDIDRKM 120

Query: 121 AVPVSNIIGHNVEGLSLWTLNPNTSILDCMEIFSKGIHRALVPVDGQVEEAVGVELVESA 180
           AVPVSNIIGHNVEGLSLWTLNPNTSILDCMEIFSKGIHRALVPVDGQVEEAVGVELVESA
Sbjct: 121 AVPVSNIIGHNVEGLSLWTLNPNTSILDCMEIFSKGIHRALVPVDGQVEEAVGVELVESA 180

Query: 181 SSYRMLTQMDVLRFLRGKVAEIEGILKQSVKEMEGMVNENVMAITDKTSVIEAIKCMKSS 240
           SSYRMLTQMDVLRFLR KV EIEGIL+ SVKEMEGM+NENVMAITDKT VIEAIKCMKSS
Sbjct: 181 SSYRMLTQMDVLRFLREKVPEIEGILRLSVKEMEGMINENVMAITDKTKVIEAIKCMKSS 240

Query: 241 FLNAVPIVGSTQLGVDQQSHAQLFTGRGKKLVGTFSATDLRGCHLATLQSWLHQTALEFT 300
           FLNAVPIVGSTQLG DQQSHAQLFTGRGKKLVGTFSATDLRGC LATLQ WLH+TAL+FT
Sbjct: 241 FLNAVPIVGSTQLGGDQQSHAQLFTGRGKKLVGTFSATDLRGCQLATLQLWLHETALDFT 300

Query: 301 DLVRK--SPLLEGAGVGVRELVTCRPESSLEEVMEKVLSKHVHRIWVTDEHGLLLGLISL 360
           +   K  SPL EG  V VRELVTCRPESSLEEVMEKV+S HVHRIWVTDEHGLLLGL+SL
Sbjct: 301 ETRGKKLSPLFEGTAVAVRELVTCRPESSLEEVMEKVVSNHVHRIWVTDEHGLLLGLVSL 360

Query: 361 SDMIRVIRLSLLSKIQT 373
           SDMIRVIRLSLLS++++
Sbjct: 361 SDMIRVIRLSLLSQLES 375

BLAST of Chy7G144250 vs. NCBI nr
Match: XP_038877421.1 (SNF1-related protein kinase regulatory subunit gamma-like PV42a isoform X1 [Benincasa hispida])

HSP 1 Score: 638 bits (1645), Expect = 8.93e-229
Identity = 337/380 (88.68%), Postives = 352/380 (92.63%), Query Frame = 0

Query: 1   MQATAKRTETQAAVGEKKVKDLMGDKRRLVEVPYTASLAQTMNVLVANHVVAVPVAAPPG 60
           MQ TAKRTET+A V EKKVKDLMG+KRRLVEVPYTASLAQTMNVLVAN VVAVPVAAPPG
Sbjct: 1   MQRTAKRTETEAVV-EKKVKDLMGEKRRLVEVPYTASLAQTMNVLVANRVVAVPVAAPPG 60

Query: 61  HWIGAGGSMIMESDKRTGVLRKHYIGMVTMLDILAHIAGDD--HDGDGGRDDLIDLDRKM 120
           HWIGAGGSMIMESDKRTGVLRKHYIGMVTMLDILAHIAGDD  HD DGG D L+D+DRKM
Sbjct: 61  HWIGAGGSMIMESDKRTGVLRKHYIGMVTMLDILAHIAGDDQDHDHDGGHD-LLDIDRKM 120

Query: 121 AVPVSNIIGHNVEGLSLWTLNPNTSILDCMEIFSKGIHRALVPVDGQVEEAVGVELVESA 180
           AVPVSNIIGHNVEGLSLWTLNPNTSILDCMEIFSKGIHRALVPVDGQVEEAVGVELVESA
Sbjct: 121 AVPVSNIIGHNVEGLSLWTLNPNTSILDCMEIFSKGIHRALVPVDGQVEEAVGVELVESA 180

Query: 181 SSYRMLTQMDVLRFLRGKVAEIEGILKQSVKEMEGMVNENVMAITDKTSVIEAIKCMKSS 240
           SSYRMLTQMDVLRFLR KV EIEGIL+ SVKEMEGM+NENVMAITDKT VIEAIKCMKSS
Sbjct: 181 SSYRMLTQMDVLRFLREKVPEIEGILRLSVKEMEGMINENVMAITDKTKVIEAIKCMKSS 240

Query: 241 FLNAVPIVGSTQLGVDQQSHAQLFT-----GRGKKLVGTFSATDLRGCHLATLQSWLHQT 300
           FLNAVPIVGSTQLG DQQSHAQLFT      RGKKLVGTFSATDLRGC LATLQSWL +T
Sbjct: 241 FLNAVPIVGSTQLGGDQQSHAQLFTVNFTETRGKKLVGTFSATDLRGCQLATLQSWLLET 300

Query: 301 ALEFTDLVRKSPLLEGAGVGVRELVTCRPESSLEEVMEKVLSKHVHRIWVTDEHGLLLGL 360
           AL+FT+ VRKSPL EG  V VRELVTCRPESSLEEVMEKV+S HVHRIWVTDEHGLLLGL
Sbjct: 301 ALDFTETVRKSPLFEGTAVAVRELVTCRPESSLEEVMEKVVSNHVHRIWVTDEHGLLLGL 360

Query: 361 ISLSDMIRVIRLSLLSKIQT 373
           +SLSDMIRVIRLSLLS++++
Sbjct: 361 VSLSDMIRVIRLSLLSQLES 378

BLAST of Chy7G144250 vs. NCBI nr
Match: XP_022979620.1 (SNF1-related protein kinase regulatory subunit gamma-like PV42a [Cucurbita maxima])

HSP 1 Score: 559 bits (1440), Expect = 9.40e-198
Identity = 297/374 (79.41%), Postives = 328/374 (87.70%), Query Frame = 0

Query: 2   QATAKRTETQAAVGEKKVKDLMGDKRRLVEVPYTASLAQTMNVLVANHVVAVPVAAPPGH 61
           Q TAK+ +TQA V +KKVKDLM D RRLVEVPYTASLA TMNVLVAN VVAVPVAAPPGH
Sbjct: 3   QPTAKKPDTQAVV-DKKVKDLMRDWRRLVEVPYTASLAHTMNVLVANCVVAVPVAAPPGH 62

Query: 62  WIGAGGSMIMESDKRTGVLRKHYIGMVTMLDILAHIAG--DDHDGDGGRDDLIDLDRKMA 121
           WIGAGGSMIME+DK +GV+RKHYIGMVTMLDILAHIAG  D HD  G   DL+DLDRKMA
Sbjct: 63  WIGAGGSMIMEADKSSGVVRKHYIGMVTMLDILAHIAGSEDVHDSSG---DLLDLDRKMA 122

Query: 122 VPVSNIIGHNVEGLSLWTLNPNTSILDCMEIFSKGIHRALVPVDGQVEEAVGVELVESAS 181
           VPVS+IIGH+VEGLSLWTLNPNTSILDCMEIFSKGIHRALVP+DGQVEEA+GV+LVESA+
Sbjct: 123 VPVSSIIGHSVEGLSLWTLNPNTSILDCMEIFSKGIHRALVPIDGQVEEAMGVKLVESAT 182

Query: 182 SYRMLTQMDVLRFLRGKVAEIEGILKQSVKEMEGMVNENVMAITDKTSVIEAIKCMKSSF 241
           SYRM+TQMDVLRFLR K  EI+ IL+QSVKEMEG +NENVMAITDKT +IEAIKCMKSSF
Sbjct: 183 SYRMVTQMDVLRFLREKAPEIKEILRQSVKEMEG-INENVMAITDKTKLIEAIKCMKSSF 242

Query: 242 LNAVPIVGSTQLGVDQQSHAQLFTGRGKKLVGTFSATDLRGCHLATLQSWLHQTALEFTD 301
           LNAVPIVG   +      H QLFTGRG KLVGTFSATDLRGC LATLQ+WLH++ L+FTD
Sbjct: 243 LNAVPIVGGDHV------HTQLFTGRGMKLVGTFSATDLRGCQLATLQTWLHRSVLDFTD 302

Query: 302 LVRKSPLLEGAGVGVRELVTCRPESSLEEVMEKVLSKHVHRIWVTDEHGLLLGLISLSDM 361
            VR S L EG+    RELVTC+PESSLEEVMEKV+SKHVHRIWV DEHGLLLG++SLSDM
Sbjct: 303 AVRNSSLFEGSVAFGRELVTCQPESSLEEVMEKVVSKHVHRIWVIDEHGLLLGVVSLSDM 362

Query: 362 IRVIRLSLLSKIQT 373
           IRV+R SLLS++QT
Sbjct: 363 IRVVRHSLLSQLQT 365

BLAST of Chy7G144250 vs. TAIR 10
Match: AT1G15330.1 (Cystathionine beta-synthase (CBS) protein )

HSP 1 Score: 387.5 bits (994), Expect = 1.2e-107
Identity = 207/366 (56.56%), Postives = 270/366 (73.77%), Query Frame = 0

Query: 6   KRTETQAAVGEKKVKDLMGDKRRLVEVPYTASLAQTMNVLVANHVVAVPVAAPPGHWIGA 65
           K  E  + +     KDL    RRLVEVPYTA+L+  MN LVAN + A+PVAAPPGHWIGA
Sbjct: 5   KSKEDHSRLINVTAKDLTVRNRRLVEVPYTATLSHAMNTLVANSISALPVAAPPGHWIGA 64

Query: 66  GGSMIMESDKRTGVLRKHYIGMVTMLDILAHIAGDDHDGDGGRDDLIDLDRKMAVPVSNI 125
           GGSMIMESDK+TGV+RKHYIG++TMLDILAHIAG+D        +L DLDRKM+  VS+I
Sbjct: 65  GGSMIMESDKQTGVVRKHYIGILTMLDILAHIAGED-------SNLSDLDRKMSSQVSSI 124

Query: 126 IGHNVEGLSLWTLNPNTSILDCMEIFSKGIHRALVPVDGQVEE---AVGVELVESASSYR 185
           IGH +EGLSLWTLNPNTS+L+CME+FSKGIHRALVPV+  +E      GVEL+ESAS+Y+
Sbjct: 125 IGHCLEGLSLWTLNPNTSVLECMEVFSKGIHRALVPVESSIESNNTIAGVELIESASAYK 184

Query: 186 MLTQMDVLRFLRG-KVAEIEGILKQSVKEMEGMVNENVMAITDKTSVIEAIKCMKSSFLN 245
           MLTQMD+LRFL+     +++ +L +S+ ++ G VN++V AIT++T+V  AI  MK + LN
Sbjct: 185 MLTQMDLLRFLKDHHFDDLKTVLSRSISDL-GAVNDSVYAITERTTVSNAINVMKGALLN 244

Query: 246 AVPIVGSTQLGVDQQSHAQLFTGRGKKLVGTFSATDLRGCHLATLQSWLHQTALEFTDLV 305
           AVPIV +  +   Q+ H QL  GR +K++GTFSATDL+GC L  LQ+WL  TALEFT   
Sbjct: 245 AVPIVHAPDIA--QEDHLQLVNGRHRKVIGTFSATDLKGCRLPELQTWLPLTALEFT--- 304

Query: 306 RKSPLLEGAGVGVRELVTCRPESSLEEVMEKVLSKHVHRIWVTDEHGLLLGLISLSDMIR 365
                 E      RE+V+C  ES++EE +EKV+++ VHR+WV D+ GLL G++SL+D+IR
Sbjct: 305 ------EKTSGKEREVVSCGVESTMEEAIEKVVTRGVHRVWVMDQQGLLQGVVSLTDIIR 351

Query: 366 VIRLSL 368
            +R +L
Sbjct: 365 SLRSTL 351

BLAST of Chy7G144250 vs. TAIR 10
Match: AT1G80090.1 (Cystathionine beta-synthase (CBS) family protein )

HSP 1 Score: 363.2 bits (931), Expect = 2.5e-100
Identity = 196/360 (54.44%), Postives = 256/360 (71.11%), Query Frame = 0

Query: 18  KVKDLMGDKRRLVEVPYTASLAQTMNVL-------VANHVVAVPVAAPPGHWIGAGGSMI 77
           KVKDLM DKRRLVEVP  A+L   +N +       VAN V AVPVAA PG W+GAGGSMI
Sbjct: 49  KVKDLMIDKRRLVEVPDNATLGDALNTMTILGIKPVANRVRAVPVAAKPGQWLGAGGSMI 108

Query: 78  MESDKRTGVLRKHYIGMVTMLDILAHIAGDDHDGDGGRDDLIDLDRKMAVPVSNIIGHNV 137
           +E DK++G  RK YIGMVTMLD++AHIAGD  DG+ G      LD+KMA PVS+IIGH  
Sbjct: 109 VELDKQSGSARKQYIGMVTMLDVVAHIAGD--DGESG------LDKKMAAPVSSIIGHCP 168

Query: 138 EGLSLWTLNPNTSILDCMEIFSKGIHRALVPVDGQVEEAVGVELVESASSYRMLTQMDVL 197
           EGLSLW+LNPNTSI+DCME+ SKGIHR LVP+D   E   G ELVESAS+Y ML+QMD++
Sbjct: 169 EGLSLWSLNPNTSIMDCMEMLSKGIHRVLVPLDSNTENITGPELVESASAYAMLSQMDLI 228

Query: 198 RFLRGKVAEIEGILKQSVKEMEGMVNENVMAITDKTSVIEAIKCMKSSFLNAVPIVGSTQ 257
            F   + +++ GIL  +V ++   ++  V+A+T +  V +AI+CM  + LNAVPIV ++ 
Sbjct: 229 SFFFDQSSQLHGILSHTVTDLSA-IHNTVLALTSQARVKDAIQCMSIAMLNAVPIVEASG 288

Query: 258 LGVDQQSHAQLFTGRGKKLVGTFSATDLRGCHLATLQSWLHQTALEFTDLVRKSPLLEGA 317
            G D   H QL  G+ +++VGTFSA+DL+GCHLATL+SWL   ALEF + + ++ L   A
Sbjct: 289 EGED---HKQLVDGKNRRVVGTFSASDLKGCHLATLRSWLPLNALEFVEKIPRTLLFTAA 348

Query: 318 -GVGVRELVTCRPESSLEEVMEKVLSKHVHRIWVTDEHGLLLGLISLSDMIRVIRLSLLS 370
                RELVTC   S+L +V+  V +K VHR+WV D++G L GL+SL+D+I V+R +LLS
Sbjct: 349 TSTPGRELVTCHVTSTLAQVIHMVTTKRVHRVWVVDQNGGLQGLVSLTDIIAVVRSALLS 396

BLAST of Chy7G144250 vs. TAIR 10
Match: AT1G65320.1 (Cystathionine beta-synthase (CBS) family protein )

HSP 1 Score: 57.0 bits (136), Expect = 3.8e-08
Identity = 83/425 (19.53%), Postives = 161/425 (37.88%), Query Frame = 0

Query: 19  VKDLMGDKRRLVEVPYTASLAQTMNVLVANHVVAVPVAAPPGHWIGAGGSMIMESDKRTG 78
           V DL   K  +VE   T ++   +  +  +    +PV      W       +    + + 
Sbjct: 10  VGDLTVGKPEMVEFYETETVESAIRAIGESTECGIPV------WRKRTTPSLPGFVENSE 69

Query: 79  VLRKHYIGMVTMLDILAHIAGDDHDGDGGRDDLIDLDRKMAVPVSNIIGHNVEGLSLWTL 138
           + ++ ++G++  LDI+A +A         + + +  ++ M +PVS ++  + +   L  +
Sbjct: 70  MRQQRFVGILNSLDIVAFLA---------KTECLQEEKAMKIPVSEVV--SPDNTLLKQV 129

Query: 139 NPNTSILDCMEIFSKGIHRALVP--------------------------------VDGQV 198
           +P T ++D +E+  +G+ R LVP                                +    
Sbjct: 130 DPGTRLIDALEMMKQGVRRLLVPKSVVWRGMSKRFSILYNGKWLKNSENSSSSSGLSADS 189

Query: 199 EEAVGVELVESASSYRMLTQMDVLRFLRGKVAEIEGILKQSVKEMEGMVNENVMAITDKT 258
                  +  S   +  L++ DV+RFL G +  +  +   S+  + G++N+N   I    
Sbjct: 190 TNRPTTSMTSSRDKFCCLSREDVIRFLIGVLGALAPLPLTSISTL-GIINQNYNFIEASL 249

Query: 259 SVIEAIKCMKSSFLNAVPIVGSTQLGV-DQQSHAQLFTGRGKKLVGTFSATDLRGCH--- 318
             IEA +          P+   + + V +Q  + Q F     K++G  SA+ L  C    
Sbjct: 250 PAIEATR---------RPLCDPSAIAVLEQTENEQQF-----KIIGEISASKLWKCDYLA 309

Query: 319 ----LATLQSWLHQTALE-------FTDLVRKS-PLLEGAGV------------------ 364
               LA L +      +E       F+D ++ S P  E  G                   
Sbjct: 310 AAWALANLYAGQFVMGVEDNMSSRSFSDFLQTSFPGGEQNGTATNAKKFSSRSIGFNPTS 369

BLAST of Chy7G144250 vs. TAIR 10
Match: AT1G69800.1 (Cystathionine beta-synthase (CBS) protein )

HSP 1 Score: 49.7 bits (117), Expect = 6.0e-06
Identity = 40/150 (26.67%), Postives = 70/150 (46.67%), Query Frame = 0

Query: 214 MVNENVMAITDKTSVIEAIKCMKSSFLNAVPIVGSTQLGVDQQSHAQLFTGRGKKLVGTF 273
           M    V++I  +  ++EA K M+ + +  +P+V                 G  KK+VG  
Sbjct: 292 MSPNEVISIESEELILEAFKRMRDNNIGGLPVV----------------EGLNKKIVGNI 351

Query: 274 SATDLRGCHL--ATLQSWLHQTALEFTDLVRKSPLLEGAGVGVRELVTCRPESSLEEVME 333
           S  D+R   L      ++   T   F   +  +   E  G+ +   +TCRP+S+L  V+ 
Sbjct: 352 SMRDIRYLLLQPEVFSNFRQLTVKSFATKIATAG--EEYGLAI-PAITCRPDSTLGSVIN 411

Query: 334 KVLSKHVHRIWV-TDEHGLLLGLISLSDMI 361
            + S+ VHR++V   +   L G+I+L D+I
Sbjct: 412 SLASRSVHRVYVAAGDENELYGVITLRDVI 422

BLAST of Chy7G144250 vs. TAIR 10
Match: AT1G69800.2 (Cystathionine beta-synthase (CBS) protein )

HSP 1 Score: 49.7 bits (117), Expect = 6.0e-06
Identity = 40/150 (26.67%), Postives = 70/150 (46.67%), Query Frame = 0

Query: 214 MVNENVMAITDKTSVIEAIKCMKSSFLNAVPIVGSTQLGVDQQSHAQLFTGRGKKLVGTF 273
           M    V++I  +  ++EA K M+ + +  +P+V                 G  KK+VG  
Sbjct: 321 MSPNEVISIESEELILEAFKRMRDNNIGGLPVV----------------EGLNKKIVGNI 380

Query: 274 SATDLRGCHL--ATLQSWLHQTALEFTDLVRKSPLLEGAGVGVRELVTCRPESSLEEVME 333
           S  D+R   L      ++   T   F   +  +   E  G+ +   +TCRP+S+L  V+ 
Sbjct: 381 SMRDIRYLLLQPEVFSNFRQLTVKSFATKIATAG--EEYGLAI-PAITCRPDSTLGSVIN 440

Query: 334 KVLSKHVHRIWV-TDEHGLLLGLISLSDMI 361
            + S+ VHR++V   +   L G+I+L D+I
Sbjct: 441 SLASRSVHRVYVAAGDENELYGVITLRDVI 451

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9XI371.7e-10656.56SNF1-related protein kinase regulatory subunit gamma-like PV42a OS=Arabidopsis t... [more]
Q8GXI92.8e-10155.52SNF1-related protein kinase regulatory subunit gamma-like PV42b OS=Arabidopsis t... [more]
Q9UGI99.7e-0925.745'-AMP-activated protein kinase subunit gamma-3 OS=Homo sapiens OX=9606 GN=PRKAG... [more]
Q9P8699.7e-0925.445'-AMP-activated protein kinase subunit gamma OS=Kluyveromyces lactis (strain AT... [more]
Q2LL382.2e-0830.715'-AMP-activated protein kinase subunit gamma-3 OS=Bos taurus OX=9913 GN=PRKAG3 ... [more]
Match NameE-valueIdentityDescription
A0A0A0KYI02.4e-20498.93Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G006180 PE=4 SV=1[more]
A0A5A7TQS52.3e-20298.40SNF1-related protein kinase regulatory subunit gamma-like PV42a OS=Cucumis melo ... [more]
A0A1S3BY752.3e-20298.40SNF1-related protein kinase regulatory subunit gamma-like PV42a OS=Cucumis melo ... [more]
A0A6J1ITT24.2e-15679.41SNF1-related protein kinase regulatory subunit gamma-like PV42a OS=Cucurbita max... [more]
A0A6J1GXB52.1e-15578.88SNF1-related protein kinase regulatory subunit gamma-like PV42a OS=Cucurbita mos... [more]
Match NameE-valueIdentityDescription
XP_004152338.23.55e-26098.93SNF1-related protein kinase regulatory subunit gamma-like PV42a [Cucumis sativus... [more]
XP_008454212.12.12e-25898.40PREDICTED: SNF1-related protein kinase regulatory subunit gamma-like PV42a [Cucu... [more]
XP_038877427.13.96e-22989.12SNF1-related protein kinase regulatory subunit gamma-like PV42a isoform X2 [Beni... [more]
XP_038877421.18.93e-22988.68SNF1-related protein kinase regulatory subunit gamma-like PV42a isoform X1 [Beni... [more]
XP_022979620.19.40e-19879.41SNF1-related protein kinase regulatory subunit gamma-like PV42a [Cucurbita maxim... [more]
Match NameE-valueIdentityDescription
AT1G15330.11.2e-10756.56Cystathionine beta-synthase (CBS) protein [more]
AT1G80090.12.5e-10054.44Cystathionine beta-synthase (CBS) family protein [more]
AT1G65320.13.8e-0819.53Cystathionine beta-synthase (CBS) family protein [more]
AT1G69800.16.0e-0626.67Cystathionine beta-synthase (CBS) protein [more]
AT1G69800.26.0e-0626.67Cystathionine beta-synthase (CBS) protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000644CBS domainSMARTSM00116cbs_1coord: 316..364
e-value: 1.8E-8
score: 44.1
coord: 218..283
e-value: 30.0
score: 5.4
IPR000644CBS domainPFAMPF00571CBScoord: 137..164
e-value: 0.0059
score: 17.0
coord: 315..363
e-value: 1.1E-11
score: 45.0
IPR000644CBS domainPROSITEPS51371CBScoord: 313..369
score: 11.784509
NoneNo IPR availableGENE3D3.10.580.10coord: 202..366
e-value: 9.1E-24
score: 85.9
NoneNo IPR availableGENE3D3.10.580.10coord: 1..201
e-value: 5.1E-12
score: 47.8
NoneNo IPR availablePANTHERPTHR13780AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNITcoord: 12..371
NoneNo IPR availablePANTHERPTHR13780:SF101SNF1-RELATED PROTEIN KINASE REGULATORY SUBUNIT GAMMA-LIKE PV42Acoord: 12..371
NoneNo IPR availableCDDcd02205CBS_pair_SFcoord: 216..350
e-value: 2.75287E-4
score: 37.9944
NoneNo IPR availableSUPERFAMILY54631CBS-domain paircoord: 183..365
NoneNo IPR availableSUPERFAMILY54631CBS-domain paircoord: 15..195

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy7G144250.1Chy7G144250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042149 cellular response to glucose starvation
biological_process GO:0006468 protein phosphorylation
biological_process GO:0050790 regulation of catalytic activity
cellular_component GO:0005737 cytoplasm
cellular_component GO:0031588 nucleotide-activated protein kinase complex
cellular_component GO:0005634 nucleus
molecular_function GO:0016208 AMP binding
molecular_function GO:0019901 protein kinase binding
molecular_function GO:0019887 protein kinase regulator activity