Chy7G143410 (gene) Cucumber (hystrix) v1

Overview
NameChy7G143410
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionDNA-directed RNA polymerases II and V subunit 8A-like
LocationchrH07: 19381506 .. 19383459 (-)
RNA-Seq ExpressionChy7G143410
SyntenyChy7G143410
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTGAGCTTCTGTTTGACGATATTTTTAAGATTGAGCGATTAAACCCTGATGGTAAAAAGTTTGATAAAGGTACATTTTTGTATAGTAGTCACTTTCATTTCATTTATACAATATTTCCAGGAATGCTTGTTAGCAGACTGTGGCAGTTTTATTGTTATATATCTGTAAAAACATTGTGCCATTCTTTAACTTTCTGATTGCGGTTAGAGATGTACTTCGTAGGCTTTTAAATCTCATCCATTCGTTATTTATTGACTTTTTAGAGGTTTCCTTTCAATCAGTTAGGATATCATCTGGAATATTATAAATTTTGTATTAAAACTCAATGTAAAATATGTGTTAAAATTTTTTTTCAACATATTTAGGCAGATGACATTTATTTTTTCACAATCTGTCCAACAGTTACTCGAATAGAGGCAAAGAGCGAGAAGTTTGACATGTTCATGCATCTAGATATAAATTCAGAGATATACCCCCTGAAAGAAGGTGAGAAGTTCTCGATGGCACTGGCTCCAACTCTGAATCTTGATGGAACCCCTGACACTGGATATTTTCTTCAGGTAACTTGTTCTTTTATTGATTTTAATCTGTTGCACACTGGATTTAATGTGGTGGTGTTGGTAAGAAAATGATGTCCTGGTAGGTTGAAGGATTTAGGCCTTGTTTGACAACTTTTTAGGCCCTGTTTAGTAGTCATTTGGTTTTTAGTTGAAAATTAAGCCTATAGACTTCAATTCCACATCTAAATTTCTTGCTGTGTTATCTACATTTTACAAGTATTTTAAAAGTGAAGCCAAAATTTGAAAACATGGAAAAGTAGTTTTTAAAAACGAAGAAATTGAGAGGAAAAGTTTAATTTTCAAAACTCAAAAAACAAAAAACGAAATAGAGTCTGAGTTTTTGGTTTTTTGTTTTTGAAAATCTTATAAATATCCCTTTGTCCTCTAAAAAATTTGTTATCTACATTAAAACCAAGCGTAAATTTGAGAACTAAAAAATGTAGTTTTAAAAAACTTGTTTTAGTTTTTAGAATTTGATTAAGAATTCAACTCTTAAGAAAAATATAAACCATTGCAAGAAATTGAGAGGAAGTAAGCTTAATCTAAAGAAACAAAAACCGAATAGTTGCCAAATGGGGTCTTAGTAGTCCATTTTGTCATAAACTACTAGTTTTGAAATATCACTTTGAACATTAATAGGGAACAAGAAACTTTCAATCTGCTGGGAAATATATGTTTTCCATAGTTATTGCATATGTCAAGACAGGTCTTCATTGTTTTCTGACAACTATATTTGTTCTTTCTTCTGTTCAAACACTAATTTGGTCTTGAGCATTTTCGACACTTCAATATGTCTACTTAACAATCATTAGGCTGCATAACAGGGCAATCGGAAGTCTCTTGCAGACAGATATGAATATGTCATGCAAGGGAAGCTGTTCAGAATTTCAGAAAGTTCTGGCCATGGCGGAAAAGCGTATGTACTATAACTTCTCGAAACAATTTTAATGTGACACTTTAATTCTACTGTATGAGCATTAGAGAACCGAGAGCTTGGCATTGCCAAATTATATTTATCTAAATTTGAGCAAGAACCTGTCACGGTGCATTCTCCTGTCTATAATGGCTACGGAAGTGTATTGCGTGTGAATAAAATTGATGTTAGTTTCTTTTCGTGAACTGTAGGAACACACACACATATATATATTCTAGTAGAATTATTATCTAAAAAAACTAAATTCATCTGAATCTAGTGAAATAGATGCTTCTTTTGGTGGACTTTTGATGATGCTGAAGGGAGACCCCTCACATTGCAGCAAGTTTGAACTTGACCAGAGGCTATTTCTTCTCATCAGGAAGGTGTGAAGCGCTTTCATTTGATTTGATCTCTAATGACTTGCTACAAAAGCTCTGGACATCGCTTATCGCTATTAGAACTGTGAATGTTTAG

mRNA sequence

ATGGTTGAGCTTCTGTTTGACGATATTTTTAAGATTGAGCGATTAAACCCTGATGGTAAAAAGTTTGATAAAGTTACTCGAATAGAGGCAAAGAGCGAGAAGTTTGACATGTTCATGCATCTAGATATAAATTCAGAGATATACCCCCTGAAAGAAGGTGAGAAGTTCTCGATGGCACTGGCTCCAACTCTGAATCTTGATGGAACCCCTGACACTGGATATTTTCTTCAGGGCAATCGGAAGTCTCTTGCAGACAGATATGAATATGTCATGCAAGGGAAGCTGTTCAGAATTTCAGAAAGTTCTGGCCATGGCGGAAAAGCGGAGACCCCTCACATTGCAGCAAGTTTGAACTTGACCAGAGGCTATTTCTTCTCATCAGGAAGGTGTGAAGCGCTTTCATTTGATTTGATCTCTAATGACTTGCTACAAAAGCTCTGGACATCGCTTATCGCTATTAGAACTGTGAATGTTTAG

Coding sequence (CDS)

ATGGTTGAGCTTCTGTTTGACGATATTTTTAAGATTGAGCGATTAAACCCTGATGGTAAAAAGTTTGATAAAGTTACTCGAATAGAGGCAAAGAGCGAGAAGTTTGACATGTTCATGCATCTAGATATAAATTCAGAGATATACCCCCTGAAAGAAGGTGAGAAGTTCTCGATGGCACTGGCTCCAACTCTGAATCTTGATGGAACCCCTGACACTGGATATTTTCTTCAGGGCAATCGGAAGTCTCTTGCAGACAGATATGAATATGTCATGCAAGGGAAGCTGTTCAGAATTTCAGAAAGTTCTGGCCATGGCGGAAAAGCGGAGACCCCTCACATTGCAGCAAGTTTGAACTTGACCAGAGGCTATTTCTTCTCATCAGGAAGGTGTGAAGCGCTTTCATTTGATTTGATCTCTAATGACTTGCTACAAAAGCTCTGGACATCGCTTATCGCTATTAGAACTGTGAATGTTTAG

Protein sequence

MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMALAPTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAETPHIAASLNLTRGYFFSSGRCEALSFDLISNDLLQKLWTSLIAIRTVNV*
Homology
[Close]
BLAST of Chy7G143410 vs. ExPASy Swiss-Prot
Match: Q9M1A8 (DNA-directed RNA polymerases II, IV and V subunit 8B OS=Arabidopsis thaliana OX=3702 GN=NRPB8B PE=1 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 5.5e-38
Identity = 68/106 (64.15%), Postives = 91/106 (85.85%), Query Frame = 0 

Query: 4   LLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMALAPT 63
           ++F+DIF +++L+PDGKKFDKVTR+EA+S   +MFMHLD+N+E+YPL  G+KF++A+APT
Sbjct: 6   IMFEDIFVVDKLDPDGKKFDKVTRVEARSHNLEMFMHLDVNTEVYPLAVGDKFTLAMAPT 65

Query: 64  LNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAE 110
           LNLDGTPDTGYF  G +K+LAD+YEY+M GKL++ISE  G   KAE
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKISERDGKTPKAE 111

[Close]
BLAST of Chy7G143410 vs. ExPASy Swiss-Prot
Match: O81097 (DNA-directed RNA polymerases II and V subunit 8A OS=Arabidopsis thaliana OX=3702 GN=NRPB8A PE=1 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 1.2e-37
Identity = 69/106 (65.09%), Postives = 90/106 (84.91%), Query Frame = 0 

Query: 4   LLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMALAPT 63
           +LF+DIF +++L+PDGKKFDKVTR++A S   +MFMHLD+N+E+YPL  G+KF++ALAPT
Sbjct: 6   ILFEDIFVVDQLDPDGKKFDKVTRVQATSHNLEMFMHLDVNTEVYPLAVGDKFTLALAPT 65

Query: 64  LNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAE 110
           LNLDGTPDTGYF  G +K+LAD+YEY+M GKL++ISE  G   KAE
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKISERDGKTPKAE 111

[Close]
BLAST of Chy7G143410 vs. ExPASy Swiss-Prot
Match: Q54YW8 (DNA-directed RNA polymerases I, II, and III subunit rpabc3 OS=Dictyostelium discoideum OX=44689 GN=polr2h PE=3 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 1.0e-20
Identity = 46/102 (45.10%), Postives = 70/102 (68.63%), Query Frame = 0 

Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60
           M  +LF+D F+++ ++PDGKKFD+V+R    SE ++M + +DI + IYPL   E+F M L
Sbjct: 1   MTSILFEDTFEVKEIDPDGKKFDRVSRFVCYSENYEMDLQIDIATHIYPLGH-ERFKMVL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESS 103
           A +LNLDG+ D       N+ SLAD+Y+YVM GK+F+  + +
Sbjct: 61  ASSLNLDGSSDNSGSYDTNKPSLADKYDYVMYGKIFKYQKEN 101

[Close]
BLAST of Chy7G143410 vs. ExPASy Swiss-Prot
Match: P52434 (DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Homo sapiens OX=9606 GN=POLR2H PE=1 SV=4)

HSP 1 Score: 100.5 bits (249), Expect = 1.8e-20
Identity = 48/99 (48.48%), Postives = 71/99 (71.72%), Query Frame = 0 

Query: 1  MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60
          M  +LF+DIF ++ ++P+GKKFD+V+R+  +SE F M + LD+N +IYP+  G+KF + +
Sbjct: 1  MAGILFEDIFDVKDIDPEGKKFDRVSRLHCESESFKMDLILDVNIQIYPVDLGDKFRLVI 60

Query: 61 APTLNLDGTPDTG-YFLQGNRKSLADRYEYVMQGKLFRI 99
          A TL  DGT D G Y    +R S AD++EYVM GK++RI
Sbjct: 61 ASTLYEDGTLDDGEYNPTDDRPSRADQFEYVMYGKVYRI 99

[Close]
BLAST of Chy7G143410 vs. ExPASy Swiss-Prot
Match: Q923G2 (DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Mus musculus OX=10090 GN=Polr2h PE=1 SV=3)

HSP 1 Score: 100.5 bits (249), Expect = 1.8e-20
Identity = 48/99 (48.48%), Postives = 71/99 (71.72%), Query Frame = 0 

Query: 1  MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60
          M  +LF+DIF ++ ++P+GKKFD+V+R+  +SE F M + LD+N +IYP+  G+KF + +
Sbjct: 1  MAGILFEDIFDVKDIDPEGKKFDRVSRLHCESESFKMDLILDVNIQIYPVDLGDKFRLVI 60

Query: 61 APTLNLDGTPDTG-YFLQGNRKSLADRYEYVMQGKLFRI 99
          A TL  DGT D G Y    +R S AD++EYVM GK++RI
Sbjct: 61 ASTLYEDGTLDDGEYNPTDDRPSRADQFEYVMYGKVYRI 99

[Close]
BLAST of Chy7G143410 vs. ExPASy TrEMBL
Match: A0A0A0KYV5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G011050 PE=3 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 6.7e-55
Identity = 109/109 (100.00%), Postives = 109/109 (100.00%), Query Frame = 0 

Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAE 110
           APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAE
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAE 109

[Close]
BLAST of Chy7G143410 vs. ExPASy TrEMBL
Match: A0A1S3BXS2 (DNA-directed RNA polymerases II and V subunit 8A-like OS=Cucumis melo OX=3656 GN=LOC103494598 PE=3 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 8.8e-55
Identity = 108/109 (99.08%), Postives = 109/109 (100.00%), Query Frame = 0 

Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLD+NSEIYPLKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDVNSEIYPLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAE 110
           APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAE
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAE 109

[Close]
BLAST of Chy7G143410 vs. ExPASy TrEMBL
Match: A0A5D3CZU6 (DNA-directed RNA polymerases II and V subunit 8A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001940 PE=3 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 3.3e-54
Identity = 106/108 (98.15%), Postives = 107/108 (99.07%), Query Frame = 0 

Query: 25  VTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMALAPTLNLDGTPDTGYFLQGNRKSLA 84
           VTRIEAKSEKFDMFMHLD+NSEIYPLKEGEKFSMALAPTLNLDGTPDTGYFLQGNRKSLA
Sbjct: 79  VTRIEAKSEKFDMFMHLDVNSEIYPLKEGEKFSMALAPTLNLDGTPDTGYFLQGNRKSLA 138

Query: 85  DRYEYVMQGKLFRISESSGHGGKAETPHIAASLNLTRGYFFSSGRCEA 133
           DRYEYVMQGKLFRISESSGHGGKAE PHIAASLNLTRGYFFSSGRCEA
Sbjct: 139 DRYEYVMQGKLFRISESSGHGGKAEIPHIAASLNLTRGYFFSSGRCEA 186

[Close]
BLAST of Chy7G143410 vs. ExPASy TrEMBL
Match: A0A6J1EXM3 (DNA-directed RNA polymerases II and V subunit 8A-like OS=Cucurbita moschata OX=3662 GN=LOC111439203 PE=3 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 6.3e-53
Identity = 106/109 (97.25%), Postives = 107/109 (98.17%), Query Frame = 0 

Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDIN+EIY LKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAE 110
           APTLNLDGTPDTGYFLQGNRKSLADRYEYVM GKLFRISESSGHGGKAE
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMHGKLFRISESSGHGGKAE 109

[Close]
BLAST of Chy7G143410 vs. ExPASy TrEMBL
Match: A0A6J1CWD5 (DNA-directed RNA polymerases II and V subunit 8A-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111015176 PE=3 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 1.1e-52
Identity = 104/109 (95.41%), Postives = 108/109 (99.08%), Query Frame = 0 

Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDIN+EIY LKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAE 110
           APTLNLDGTPDTGY+LQGNRKSLADRYEY+MQGKLFRISESSGHGG+AE
Sbjct: 61  APTLNLDGTPDTGYYLQGNRKSLADRYEYIMQGKLFRISESSGHGGRAE 109

[Close]
BLAST of Chy7G143410 vs. NCBI nr
Match: XP_004152137.1 (DNA-directed RNA polymerases II and V subunit 8A [Cucumis sativus] >KGN53006.1 hypothetical protein Csa_014470 [Cucumis sativus])

HSP 1 Score: 223 bits (568), Expect = 4.66e-72
Identity = 109/109 (100.00%), Postives = 109/109 (100.00%), Query Frame = 0 

Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAE 109
           APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAE
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAE 109

[Close]
BLAST of Chy7G143410 vs. NCBI nr
Match: XP_008454076.1 (PREDICTED: DNA-directed RNA polymerases II and V subunit 8A-like [Cucumis melo])

HSP 1 Score: 223 bits (567), Expect = 6.62e-72
Identity = 108/109 (99.08%), Postives = 109/109 (100.00%), Query Frame = 0 

Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLD+NSEIYPLKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDVNSEIYPLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAE 109
           APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAE
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAE 109

[Close]
BLAST of Chy7G143410 vs. NCBI nr
Match: XP_038879439.1 (DNA-directed RNA polymerases II and V subunit 8A-like [Benincasa hispida] >XP_038879448.1 DNA-directed RNA polymerases II and V subunit 8A-like [Benincasa hispida])

HSP 1 Score: 219 bits (559), Expect = 1.09e-70
Identity = 108/109 (99.08%), Postives = 108/109 (99.08%), Query Frame = 0 

Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIY LKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYNLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAE 109
           APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAE
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAE 109

[Close]
BLAST of Chy7G143410 vs. NCBI nr
Match: KAA0044538.1 (DNA-directed RNA polymerases II and V subunit 8A-like [Cucumis melo var. makuwa] >TYK17045.1 DNA-directed RNA polymerases II and V subunit 8A-like [Cucumis melo var. makuwa])

HSP 1 Score: 219 bits (559), Expect = 4.83e-70
Identity = 106/108 (98.15%), Postives = 107/108 (99.07%), Query Frame = 0 

Query: 25  VTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMALAPTLNLDGTPDTGYFLQGNRKSLA 84
           VTRIEAKSEKFDMFMHLD+NSEIYPLKEGEKFSMALAPTLNLDGTPDTGYFLQGNRKSLA
Sbjct: 79  VTRIEAKSEKFDMFMHLDVNSEIYPLKEGEKFSMALAPTLNLDGTPDTGYFLQGNRKSLA 138

Query: 85  DRYEYVMQGKLFRISESSGHGGKAETPHIAASLNLTRGYFFSSGRCEA 132
           DRYEYVMQGKLFRISESSGHGGKAE PHIAASLNLTRGYFFSSGRCEA
Sbjct: 139 DRYEYVMQGKLFRISESSGHGGKAEIPHIAASLNLTRGYFFSSGRCEA 186

[Close]
BLAST of Chy7G143410 vs. NCBI nr
Match: XP_022932746.1 (DNA-directed RNA polymerases II and V subunit 8A-like [Cucurbita moschata] >XP_022932747.1 DNA-directed RNA polymerases II and V subunit 8A-like [Cucurbita moschata] >XP_023538873.1 DNA-directed RNA polymerases II and V subunit 8A-like [Cucurbita pepo subsp. pepo] >XP_023538874.1 DNA-directed RNA polymerases II and V subunit 8A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 216 bits (551), Expect = 1.81e-69
Identity = 106/109 (97.25%), Postives = 107/109 (98.17%), Query Frame = 0 

Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDIN+EIY LKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAE 109
           APTLNLDGTPDTGYFLQGNRKSLADRYEYVM GKLFRISESSGHGGKAE
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMHGKLFRISESSGHGGKAE 109

[Close]
BLAST of Chy7G143410 vs. TAIR 10
Match: AT3G59600.1 (RNA polymerase Rpb8 )

HSP 1 Score: 158.7 bits (400), Expect = 3.9e-39
Identity = 68/106 (64.15%), Postives = 91/106 (85.85%), Query Frame = 0 

Query: 4   LLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMALAPT 63
           ++F+DIF +++L+PDGKKFDKVTR+EA+S   +MFMHLD+N+E+YPL  G+KF++A+APT
Sbjct: 6   IMFEDIFVVDKLDPDGKKFDKVTRVEARSHNLEMFMHLDVNTEVYPLAVGDKFTLAMAPT 65

Query: 64  LNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAE 110
           LNLDGTPDTGYF  G +K+LAD+YEY+M GKL++ISE  G   KAE
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKISERDGKTPKAE 111

[Close]
BLAST of Chy7G143410 vs. TAIR 10
Match: AT1G54250.1 (RNA polymerase Rpb8 )

HSP 1 Score: 157.5 bits (397), Expect = 8.7e-39
Identity = 69/106 (65.09%), Postives = 90/106 (84.91%), Query Frame = 0 

Query: 4   LLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMALAPT 63
           +LF+DIF +++L+PDGKKFDKVTR++A S   +MFMHLD+N+E+YPL  G+KF++ALAPT
Sbjct: 6   ILFEDIFVVDQLDPDGKKFDKVTRVQATSHNLEMFMHLDVNTEVYPLAVGDKFTLALAPT 65

Query: 64  LNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAE 110
           LNLDGTPDTGYF  G +K+LAD+YEY+M GKL++ISE  G   KAE
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKISERDGKTPKAE 111

The following BLAST results are available for this feature:
BLAST of Chy7G143410 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9M1A85.5e-3864.15DNA-directed RNA polymerases II, IV and V subunit 8B OS=Arabidopsis thaliana OX=... [more]
O810971.2e-3765.09DNA-directed RNA polymerases II and V subunit 8A OS=Arabidopsis thaliana OX=3702... [more]
Q54YW81.0e-2045.10DNA-directed RNA polymerases I, II, and III subunit rpabc3 OS=Dictyostelium disc... [more]
P524341.8e-2048.48DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Homo sapiens OX=96... [more]
Q923G21.8e-2048.48DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Mus musculus OX=10... [more]
BLAST of Chy7G143410 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KYV56.7e-55100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G011050 PE=3 SV=1[more]
A0A1S3BXS28.8e-5599.08DNA-directed RNA polymerases II and V subunit 8A-like OS=Cucumis melo OX=3656 GN... [more]
A0A5D3CZU63.3e-5498.15DNA-directed RNA polymerases II and V subunit 8A-like OS=Cucumis melo var. makuw... [more]
A0A6J1EXM36.3e-5397.25DNA-directed RNA polymerases II and V subunit 8A-like OS=Cucurbita moschata OX=3... [more]
A0A6J1CWD51.1e-5295.41DNA-directed RNA polymerases II and V subunit 8A-like isoform X2 OS=Momordica ch... [more]
BLAST of Chy7G143410 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004152137.14.66e-72100.00DNA-directed RNA polymerases II and V subunit 8A [Cucumis sativus] >KGN53006.1 h... [more]
XP_008454076.16.62e-7299.08PREDICTED: DNA-directed RNA polymerases II and V subunit 8A-like [Cucumis melo][more]
XP_038879439.11.09e-7099.08DNA-directed RNA polymerases II and V subunit 8A-like [Benincasa hispida] >XP_03... [more]
KAA0044538.14.83e-7098.15DNA-directed RNA polymerases II and V subunit 8A-like [Cucumis melo var. makuwa]... [more]
XP_022932746.11.81e-6997.25DNA-directed RNA polymerases II and V subunit 8A-like [Cucurbita moschata] >XP_0... [more]
BLAST of Chy7G143410 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G59600.13.9e-3964.15RNA polymerase Rpb8 [more]
AT1G54250.18.7e-3965.09RNA polymerase Rpb8 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005570RNA polymerase, Rpb8SMARTSM00658rpol8neucoord: 2..121
e-value: 7.2E-40
score: 148.5
IPR005570RNA polymerase, Rpb8PFAMPF03870RNA_pol_Rpb8coord: 7..104
e-value: 3.2E-33
score: 114.8
IPR005570RNA polymerase, Rpb8PANTHERPTHR10917DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3coord: 1..109
NoneNo IPR availableGENE3D2.40.50.140coord: 6..118
e-value: 6.6E-38
score: 131.9
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 4..104

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy7G143410.1Chy7G143410.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006351 transcription, DNA-templated
cellular_component GO:0005736 RNA polymerase I complex
cellular_component GO:0005665 RNA polymerase II, core complex
cellular_component GO:0005666 RNA polymerase III complex
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity