Chy7G142540 (gene) Cucumber (hystrix) v1

BLAST of Chy7G142540 vs. ExPASy Swiss-Prot
Match: Q0QLE4 (2,3-dimethylmalate lyase OS=Eubacterium barkeri OX=1528 GN=Dml PE=1 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 4.2e-52
Identity = 117/290 (40.34%), Postives = 176/290 (60.69%), Query Frame = 0

Query: 79  AKALRRILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFSISAARLALPDTGYMSYGE 138
           A  +R +L    +   P   DA++AK++ R GF   + +G+  SA+ L  PD G ++  E
Sbjct: 4   AAKMRELLSTKKMVVAPGAHDAMTAKVIGRLGFDAVYMTGYGQSASHLGQPDVGLLTMTE 63

Query: 139 MLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGFAGIILEDQVSPKACGHTQ 198
           M+ +   I ++  +PVI D D G+GNA+NV RTV+ Y +AG A I LEDQV PK CGH  
Sbjct: 64  MVARANAIVEAAGVPVIADADTGFGNAVNVMRTVREYEKAGVAVIQLEDQVMPKKCGHMV 123

Query: 199 GRKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSLSESLRRARAFADAGADVL 258
           GR++VS+EE V +IKAAVD R     D +I+ARTD+R    + E+L R  A+ +AGAD++
Sbjct: 124 GREIVSKEEMVGKIKAAVDTR--VNPDFMIMARTDARTTKGIDEALERGLAYKEAGADII 183

Query: 259 FIDALASKDEMDAFCKISPKVP--KMANMLEGGGKTPILNPLELEEMGFKIVAYPLSLVG 318
           FI++   ++EM    +I+  +P   +ANM+E GG+TP+L   ELE +G+ I  YP + + 
Sbjct: 184 FIESPEGEEEMK---RINETIPGYTLANMVE-GGRTPLLKNAELEALGYNITIYPTASIY 243

Query: 319 VSIRAMQDALLAIKGGRLPS--PGTLPTFAEMKELLGFNSYYEEERKYAS 365
           V+ +AM D   A+K     +    T+ TF+E  +L+G     E E  YA+
Sbjct: 244 VATKAMVDLWTALKNDDTTAGVMDTMVTFSEFNDLMGLEKIREVEHNYAT 287

BLAST of Chy7G142540 vs. ExPASy Swiss-Prot
Match: Q05957 (Petal death protein OS=Dianthus caryophyllus OX=3570 GN=PDP PE=1 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 4.8e-48
Identity = 118/286 (41.26%), Postives = 174/286 (60.84%), Query Frame = 0

Query: 82  LRRILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFSISAARLALPDTGYMSYGEMLD 141
           + R++E  G    P   DALSA +VE+ GF   F SG+S+SAA L LPD G ++  E+++
Sbjct: 31  MHRLIEEHGSVLMPGVQDALSAAVVEKTGFHAAFVSGYSVSAAMLGLPDFGLLTTTEVVE 90

Query: 142 QGQLITQSV-SIPVIGDGDNGYGNAMNVKRTVKGYIRAGFAGIILEDQVSPKACGHTQGR 201
             + IT +  ++ V+ DGD G G  +NV+R ++  I AG  G+ LEDQV PK CGH +G+
Sbjct: 91  ATRRITAAAPNLCVVVDGDTGGGGPLNVQRFIRELISAGAKGVFLEDQVWPKKCGHMRGK 150

Query: 202 KVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSLSESLRRARAFADAGADVLFI 261
            VV  EE  L+I AA +A  +  SD  +VARTD+R    L E +RRA  + +AGAD  F+
Sbjct: 151 AVVPAEEHALKIAAAREAIGD--SDFFLVARTDARAPHGLEEGIRRANLYKEAGADATFV 210

Query: 262 DALASKDEMDAFCKISPKVP--KMANMLEGGGKTPILNPLELEEMGFKIVAYPLSLVGVS 321
           +A A+ DE+    ++S K    ++ANM+E GGKTP+  P E +EMGF ++A+ L+ V  +
Sbjct: 211 EAPANVDELK---EVSAKTKGLRIANMIE-GGKTPLHTPEEFKEMGFHLIAHSLTAVYAT 270

Query: 322 IRAMQD--ALLAIKGGRLPSPGTLPTFAEMKELLGFNSYYEEERKY 363
            RA+ +   +L  KG        + TF+E  EL+   S+YE E K+
Sbjct: 271 ARALVNIMKILKEKGTTRDDLDQMATFSEFNELISLESWYEMESKF 310

BLAST of Chy7G142540 vs. ExPASy Swiss-Prot
Match: O49290 (Carboxyvinyl-carboxyphosphonate phosphorylmutase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g77060 PE=1 SV=2)

HSP 1 Score: 188.3 bits (477), Expect = 2.0e-46
Identity = 121/335 (36.12%), Postives = 187/335 (55.82%), Query Frame = 0

Query: 36  LSLSWRPSIKRNISLSNNPHHKPLSRVMAMSARINDEYVVESPAKALRRILEMPGVHQGP 95
           L+L+  P+ +RN            +R++  +ARI   +          R++E  G+   P
Sbjct: 17  LNLTASPTFRRN---------PRAARLVNPTARIQTRF---------HRLIEEQGIVLMP 76

Query: 96  ACFDALSAKLVERAGFLYCFTSGFSISAARLALPDTGYMSYGEMLDQGQLITQSV-SIPV 155
            C+DALSA +V++ GF   F SG+++SA+ L  PD G ++  EM    + +  S  +IP+
Sbjct: 77  GCYDALSAAIVQQTGFSAGFISGYALSASLLGKPDFGLLTPPEMAATARSVCASAPNIPI 136

Query: 156 IGDGDNGYGNAMNVKRTVKGYIRAGFAGIILEDQVSPKACGHTQGRKVVSREEAVLRIKA 215
           I D D G GNA+N++RTVK  I AG AG  LEDQ  PK CGH +G++V+  EE   +I +
Sbjct: 137 IADADTGGGNALNIQRTVKDLIAAGAAGCFLEDQAWPKKCGHMRGKQVIPAEEHAAKIAS 196

Query: 216 AVDARKESGSDIVIVARTDSRQAVS---LSESLRRARAFADAGADVLFIDALASKDEMDA 275
           A DA  +  SD  +VARTD R   +   L +++ R   + +AGAD  F++A    DE+  
Sbjct: 197 ARDAIGD--SDFFLVARTDVRATSAKSGLEDAIARVNLYMEAGADASFVEAPRDDDELKE 256

Query: 276 FCKISPKVPKMANMLEGGGKTPILNPLELEEMGFKIVAYPLSLVGVSIRAMQDALLAIK- 335
             K   K  ++ NM+E GG TP+  P EL+EMGF ++ +PL+ +  S RA+ D L  +K 
Sbjct: 257 IGK-RTKGYRVCNMIE-GGVTPLHTPDELKEMGFHLIVHPLTALYASTRALVDVLKTLKE 316

Query: 336 -GGRLPSPGTLPTFAEMKELLGFNSYYEEERKYAS 365
            G        + TF E   L+  +S++E E +Y++
Sbjct: 317 NGSTRDHLQKMATFEEFNSLVDLDSWFELEARYSN 329

BLAST of Chy7G142540 vs. ExPASy Swiss-Prot
Match: P11435 (Carboxyvinyl-carboxyphosphonate phosphorylmutase OS=Streptomyces hygroscopicus OX=1912 GN=bcpA PE=1 SV=3)

HSP 1 Score: 186.8 bits (473), Expect = 5.9e-46
Identity = 107/289 (37.02%), Postives = 168/289 (58.13%), Query Frame = 0

Query: 79  AKALRRILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFSISAARLALPDTGYMSYGE 138
           A+  R ++  P +   P+ +DALSAK++++AGF     +G   SA+ L LPD G+ S  E
Sbjct: 6   ARTFRELMNAPEILVVPSAYDALSAKVIQQAGFPAVHMTGSGTSASMLGLPDLGFTSVSE 65

Query: 139 MLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGFAGIILEDQVSPKACGHTQ 198
                + I  +V +PVI D D GYGNAM+V R  + + R G  G  LEDQV+PK CGH +
Sbjct: 66  QAINLKNIVLTVDVPVIMDADAGYGNAMSVWRATREFERVGIVGYHLEDQVNPKRCGHLE 125

Query: 199 GRKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSLSESLRRARAFADAGADVL 258
           G++++S EE   +I+AAV+AR++   D  I+ARTD+R++  L E++RR+R +  AGAD +
Sbjct: 126 GKRLISTEEMTGKIEAAVEARED--EDFTIIARTDARESFGLDEAIRRSREYVAAGADCI 185

Query: 259 FIDALASKDEMDAFCKISPKVPKMANMLEGGGKTPILNPLELEEMGFKIVAYPLSLVGVS 318
           F++A+   +EM    +     P +ANM+E GGKTP L   ELE +G+ +  YPLS    +
Sbjct: 186 FLEAMLDVEEMKR-VRDEIDAPLLANMVE-GGKTPWLTTKELESIGYNLAIYPLSGWMAA 245

Query: 319 IRAMQDALLAIKGGRLPSP-----GTLPTFAEMKELLGFNSYYEEERKY 363
              ++     ++            G   +FAE+ E+  ++   E E ++
Sbjct: 246 ASVLRKLFTELREAGTTQKFWDDMGLKMSFAELFEVFEYSKISELEARF 290

BLAST of Chy7G142540 vs. ExPASy Swiss-Prot
Match: Q9YFM7 (2-methylisocitrate lyase OS=Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) OX=272557 GN=prpB PE=3 SV=2)

HSP 1 Score: 180.3 bits (456), Expect = 5.5e-44
Identity = 112/306 (36.60%), Postives = 175/306 (57.19%), Query Frame = 0

Query: 75  VESPAKALRRILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFSISAARLALPDTGYM 134
           +E P   LR ++E   +   P  ++   A L ER GF   + SG +I+ + LA+PD G +
Sbjct: 9   LERPGLVLRELIEKRDIVVAPGVYNPAVALLAERMGFEALYLSGAAITGS-LAMPDLGLI 68

Query: 135 SYGEMLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGFAGIILEDQVSPKAC 194
           +  E+      IT+ V +PVI D D G+G A+NV+RTV+   RAG A I +EDQV PK C
Sbjct: 69  TLSELAMFTSYITRVVRVPVIVDADTGFGEAINVERTVRELERAGAAAIQIEDQVMPKKC 128

Query: 195 GHTQGRKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSLSESLRRARAFADAG 254
           GH QG+ ++S E+ V +I AAV AR+    D +IVARTD+R      +++ RA+ + +AG
Sbjct: 129 GHLQGKALISPEDMVKKIIAAVGARR----DALIVARTDARGVEGFEKAVERAQLYVEAG 188

Query: 255 ADVLFIDALASKDEMDAFCKISPKVPKMANMLEGGGKTPILNPLELEEMGFKIVAYPLSL 314
           AD++F +AL S +E   F +   K P +ANM E  GKTP +   +  E G+KIV +P++ 
Sbjct: 189 ADIIFPEALTSLEEFREFAR-RVKAPLLANMTE-FGKTPYITVDQFREAGYKIVIFPVTT 248

Query: 315 VGVSIRAMQDALLAI--KGGRLPSPGTLPTFAEMKELLGFNSYYEEERKYASAVSQPSIK 374
              S++A +  L  I  KG +      L T  E  +L+G++ Y + + + +    +   +
Sbjct: 249 FRASLKASETVLREIMEKGTQKDILDKLYTRTEFYDLIGYHDYEKRDAEVSRKAEELLAR 307

Query: 375 VDSSIT 379
            ++S T
Sbjct: 309 HNNSRT 307

BLAST of Chy7G142540 vs. ExPASy TrEMBL
Match: A0A0A0KU11 (Isocitrate lyase OS=Cucumis sativus OX=3659 GN=Csa_4G017030 PE=4 SV=1)

HSP 1 Score: 944.9 bits (2441), Expect = 1.4e-271
Identity = 485/496 (97.78%), Postives = 489/496 (98.59%), Query Frame = 0

Query: 1   MKLPVASKDGSLSLPLSHTESLRLQLPSPPPHSFPLSLSWRPSIKRNISLSNNPHHKPLS 60
           MKLPVASKDGSL+LPLSHT SLRLQLPS PPHS P SLSWRPS+K NISLSNNP+HKPLS
Sbjct: 1   MKLPVASKDGSLTLPLSHTASLRLQLPSLPPHSIPRSLSWRPSVKGNISLSNNPYHKPLS 60

Query: 61  RVMAMSARINDEYVVESPAKALRRILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFS 120
           RVMAMSARINDE VVESPAKALRRILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFS
Sbjct: 61  RVMAMSARINDESVVESPAKALRRILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFS 120

Query: 121 ISAARLALPDTGYMSYGEMLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGF 180
           ISAARLALPDTGYMSYGEMLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGF
Sbjct: 121 ISAARLALPDTGYMSYGEMLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGF 180

Query: 181 AGIILEDQVSPKACGHTQGRKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSL 240
           AGIILEDQVSPKACGHTQGRKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSL
Sbjct: 181 AGIILEDQVSPKACGHTQGRKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSL 240

Query: 241 SESLRRARAFADAGADVLFIDALASKDEMDAFCKISPKVPKMANMLEGGGKTPILNPLEL 300
           SESLRRARAFADAGADVLFIDALASKDEM+AFCKISPKVPKMANMLEGGGKTPILNPLEL
Sbjct: 241 SESLRRARAFADAGADVLFIDALASKDEMEAFCKISPKVPKMANMLEGGGKTPILNPLEL 300

Query: 301 EEMGFKIVAYPLSLVGVSIRAMQDALLAIKGGRLPSPGTLPTFAEMKELLGFNSYYEEER 360
           EEMGFKIVAYPLSLVGVSIRAMQDALLAIKGGRLPSPGTLPTFAEMKELLGFNSYYEEER
Sbjct: 301 EEMGFKIVAYPLSLVGVSIRAMQDALLAIKGGRLPSPGTLPTFAEMKELLGFNSYYEEER 360

Query: 361 KYASAVSQPSIKVDSSITSLQRRVEDDKEKGQSSQGPAVEVITPEIYRSYDDDGSRGPFS 420
           KYASAVSQPSIKVDSSITSLQRRVEDDKEKGQ SQGPAVEVITPEIYRSYDDDGSRGPFS
Sbjct: 361 KYASAVSQPSIKVDSSITSLQRRVEDDKEKGQGSQGPAVEVITPEIYRSYDDDGSRGPFS 420

Query: 421 GIWSRRLRVKITGRDGFERLDVRIPAGFLEGLTNIVPALGGVNIKELMDDAAGEVGGKQL 480
           GIWSRRLRVKITGRDGFERLDVRIPAGFLEGLTNIVPALGGVNIKELMDDAAGEVGGKQL
Sbjct: 421 GIWSRRLRVKITGRDGFERLDVRIPAGFLEGLTNIVPALGGVNIKELMDDAAGEVGGKQL 480

Query: 481 LDFVDGMGDRIEVFLE 497
           LDFVDGMGDRIEVFLE
Sbjct: 481 LDFVDGMGDRIEVFLE 496

BLAST of Chy7G142540 vs. ExPASy TrEMBL
Match: A0A1S3BXJ9 (Isocitrate lyase OS=Cucumis melo OX=3656 GN=LOC103494513 PE=4 SV=1)

HSP 1 Score: 932.6 bits (2409), Expect = 7.0e-268
Identity = 480/497 (96.58%), Postives = 487/497 (97.99%), Query Frame = 0

Query: 1   MKLPVASKDGSLSLPLSHTESLRLQLPSPPPHSFPLSLSWRPSIKRNISLSNNPHHKPLS 60
           MKLPVASKDGSLSLPLSH  SLRL LPSPPPHS P SLSWRPSIKRNISLSNNPHHKPLS
Sbjct: 1   MKLPVASKDGSLSLPLSHIASLRLHLPSPPPHSLPRSLSWRPSIKRNISLSNNPHHKPLS 60

Query: 61  RVMAMSARINDEYVVESPAKALRRILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFS 120
           RVMAMSARINDE +VESPAKALRRILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFS
Sbjct: 61  RVMAMSARINDESIVESPAKALRRILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFS 120

Query: 121 ISAARLALPDTGYMSYGEMLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGF 180
           ISAARLALPDTGY+SYGEMLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGF
Sbjct: 121 ISAARLALPDTGYISYGEMLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGF 180

Query: 181 AGIILEDQVSPKACGHTQGRKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSL 240
           AGIILEDQVSPKACGHTQGRKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSL
Sbjct: 181 AGIILEDQVSPKACGHTQGRKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSL 240

Query: 241 SESLRRARAFADAGADVLFIDALASKDEMDAFCKISPKVPKMANMLEGGGKTPILNPLEL 300
           SESLRRARAFADAGADVLFIDALASKDEM+AFCKISPKVPKMANMLEGGGKTPIL+PLEL
Sbjct: 241 SESLRRARAFADAGADVLFIDALASKDEMEAFCKISPKVPKMANMLEGGGKTPILSPLEL 300

Query: 301 EEMGFKIVAYPLSLVGVSIRAMQDALLAIKGGRLPSPGTLPTFAEMKELLGFNSYYEEER 360
           EEMGFKIVAYPLSLVGVSIRAMQDALLAIKGGRLPSPGTLPTFAEMKELLGFNSYYEEER
Sbjct: 301 EEMGFKIVAYPLSLVGVSIRAMQDALLAIKGGRLPSPGTLPTFAEMKELLGFNSYYEEER 360

Query: 361 KYASAVSQPSIKVDSSITSLQRRVEDDKE-KGQSSQGPAVEVITPEIYRSYDDDGSRGPF 420
           KYASAVSQPSIKVDSSITSLQR+VED+KE KGQS  GPAVEVITPEI RSYDDDGSRGPF
Sbjct: 361 KYASAVSQPSIKVDSSITSLQRKVEDNKEKKGQSPPGPAVEVITPEIVRSYDDDGSRGPF 420

Query: 421 SGIWSRRLRVKITGRDGFERLDVRIPAGFLEGLTNIVPALGGVNIKELMDDAAGEVGGKQ 480
           SGIWSRRLRVKITGRDGFERLDVRIPAGFL+GLTNIVPALGGVNIKELMDDAAGEVGGKQ
Sbjct: 421 SGIWSRRLRVKITGRDGFERLDVRIPAGFLDGLTNIVPALGGVNIKELMDDAAGEVGGKQ 480

Query: 481 LLDFVDGMGDRIEVFLE 497
           LLDFVDGMGDRIEVFLE
Sbjct: 481 LLDFVDGMGDRIEVFLE 497

BLAST of Chy7G142540 vs. ExPASy TrEMBL
Match: A0A5D3CZH8 (Isocitrate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001010 PE=4 SV=1)

HSP 1 Score: 919.1 bits (2374), Expect = 8.0e-264
Identity = 476/497 (95.77%), Postives = 483/497 (97.18%), Query Frame = 0

Query: 1   MKLPVASKDGSLSLPLSHTESLRLQLPSPPPHSFPLSLSWRPSIKRNISLSNNPHHKPLS 60
           MKLPVASKDGSLSLPLSH  SLRL LPSPPPHS P SLSWRPSIKRNISLSNNPHHKPLS
Sbjct: 1   MKLPVASKDGSLSLPLSHIASLRLHLPSPPPHSLPRSLSWRPSIKRNISLSNNPHHKPLS 60

Query: 61  RVMAMSARINDEYVVESPAKALRRILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFS 120
           RVMAMSARINDE +VESPAKALRRILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFS
Sbjct: 61  RVMAMSARINDESIVESPAKALRRILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFS 120

Query: 121 ISAARLALPDTGYMSYGEMLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGF 180
           ISAARLALPDTGY+SYGEMLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGF
Sbjct: 121 ISAARLALPDTGYISYGEMLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGF 180

Query: 181 AGIILEDQVSPKACGHTQGRKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSL 240
           AGIILEDQVSPKACGHTQGRKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSL
Sbjct: 181 AGIILEDQVSPKACGHTQGRKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSL 240

Query: 241 SESLRRARAFADAGADVLFIDALASKDEMDAFCKISPKVPKMANMLEGGGKTPILNPLEL 300
           SESLRRARAFADAGADVLFIDALASKDEM+AFCKISPKVPKMANMLEGGGKTPIL+PLEL
Sbjct: 241 SESLRRARAFADAGADVLFIDALASKDEMEAFCKISPKVPKMANMLEGGGKTPILSPLEL 300

Query: 301 EEMGFKIVAYPLSLVGVSIRAMQDALLAIKGGRLPSPGTLPTFAEMKELLGFNSYYEEER 360
           EEMGFKIVAYPLSLVGVSIRAMQDALLAIKGGRLPSPGTLPTFAEMKELLGFNSYYEEER
Sbjct: 301 EEMGFKIVAYPLSLVGVSIRAMQDALLAIKGGRLPSPGTLPTFAEMKELLGFNSYYEEER 360

Query: 361 KYASAVSQPSIKVDSSITSLQRRVEDDKE-KGQSSQGPAVEVITPEIYRSYDDDGSRGPF 420
           KYASAVSQPSIKVDSSITSLQR+VED+KE KGQS  GPAVEVITPEI RSYDDDGSRGPF
Sbjct: 361 KYASAVSQPSIKVDSSITSLQRKVEDNKEKKGQSPPGPAVEVITPEIVRSYDDDGSRGPF 420

Query: 421 SGIWSRRLRVKITGRDGFERLDVRIPAGFLEGLTNIVPALGGVNIKELMDDAAGEVGGKQ 480
           SGIWSRRLRVKITGRDGFERLD    AGFL+GLTNIVPALGGVNIKELMDDAAGEVGGKQ
Sbjct: 421 SGIWSRRLRVKITGRDGFERLD----AGFLDGLTNIVPALGGVNIKELMDDAAGEVGGKQ 480

Query: 481 LLDFVDGMGDRIEVFLE 497
           LLDFVDGMGDRIEVFLE
Sbjct: 481 LLDFVDGMGDRIEVFLE 493

BLAST of Chy7G142540 vs. ExPASy TrEMBL
Match: A0A6J1GWR7 (Isocitrate lyase OS=Cucurbita moschata OX=3662 GN=LOC111457859 PE=4 SV=1)

HSP 1 Score: 877.5 bits (2266), Expect = 2.7e-251
Identity = 450/497 (90.54%), Postives = 470/497 (94.57%), Query Frame = 0

Query: 1   MKLPVASKDGSLSLPLSHTESLRLQLPSPPPHSFPLSLSWRPSIKRNISLSNNPHHKPLS 60
           M+L VASKD  LSLPLSH   LR QLPSPPPHS  LS SWRPSIKR ISLS NPH K +S
Sbjct: 1   MELRVASKDACLSLPLSHRSPLRFQLPSPPPHSLHLSPSWRPSIKRTISLSRNPHQKRVS 60

Query: 61  RVMAMSARINDEYVVESPAKALRRILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFS 120
           ++MAMSAR NDE++ ESPAKALRRILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFS
Sbjct: 61  QIMAMSARNNDEFMAESPAKALRRILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFS 120

Query: 121 ISAARLALPDTGYMSYGEMLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGF 180
           ISAARLALPDTG++SYGEMLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGF
Sbjct: 121 ISAARLALPDTGFISYGEMLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGF 180

Query: 181 AGIILEDQVSPKACGHTQGRKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSL 240
           AGIILEDQVSPKACGHTQGRKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSL
Sbjct: 181 AGIILEDQVSPKACGHTQGRKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSL 240

Query: 241 SESLRRARAFADAGADVLFIDALASKDEMDAFCKISPKVPKMANMLEGGGKTPILNPLEL 300
           SESL RARAFADAGADVLFIDALASKDEM+AFCKISPK+PKMANMLEGGGKTPI NPLEL
Sbjct: 241 SESLWRARAFADAGADVLFIDALASKDEMEAFCKISPKIPKMANMLEGGGKTPIFNPLEL 300

Query: 301 EEMGFKIVAYPLSLVGVSIRAMQDALLAIKGGRLPSPGTLPTFAEMKELLGFNSYYEEER 360
           EE+G+K+VAYPLSLVGVSIRAMQDALLAIKGGRLPSPGTLPTF EMKE+LGFNSYYEEER
Sbjct: 301 EEIGYKLVAYPLSLVGVSIRAMQDALLAIKGGRLPSPGTLPTFGEMKEILGFNSYYEEER 360

Query: 361 KYASAVSQPSIKVDSSITSLQRRVEDDKE-KGQSSQGPAVEVITPEIYRSYDDDGSRGPF 420
           KYASAVSQPSIKV+S+I+SLQRRVEDD E K ++ QGP VEVITPEIYRSYD D S+GPF
Sbjct: 361 KYASAVSQPSIKVESNISSLQRRVEDDSEKKDENPQGPVVEVITPEIYRSYDADESKGPF 420

Query: 421 SGIWSRRLRVKITGRDGFERLDVRIPAGFLEGLTNIVPALGGVNIKELMDDAAGEVGGKQ 480
           SGIWSRRLRVKITGRDGFERLDVRIPAGFLEGLTNIVPALGGVNIKELMDDAAGEVGGK 
Sbjct: 421 SGIWSRRLRVKITGRDGFERLDVRIPAGFLEGLTNIVPALGGVNIKELMDDAAGEVGGKL 480

Query: 481 LLDFVDGMGDRIEVFLE 497
           LLDFVDGMGDRIEVFLE
Sbjct: 481 LLDFVDGMGDRIEVFLE 497

BLAST of Chy7G142540 vs. ExPASy TrEMBL
Match: A0A6J1IXH9 (Isocitrate lyase OS=Cucurbita maxima OX=3661 GN=LOC111479372 PE=4 SV=1)

HSP 1 Score: 874.8 bits (2259), Expect = 1.7e-250
Identity = 450/497 (90.54%), Postives = 468/497 (94.16%), Query Frame = 0

Query: 1   MKLPVASKDGSLSLPLSHTESLRLQLPSPPPHSFPLSLSWRPSIKRNISLSNNPHHKPLS 60
           M+L VASKD  LSLPLSH   LR QLPSPPPHS  LS SWRPSIKR ISL  NPH K +S
Sbjct: 1   MELRVASKDACLSLPLSHRSPLRFQLPSPPPHSLHLSPSWRPSIKRAISLFRNPHQKRVS 60

Query: 61  RVMAMSARINDEYVVESPAKALRRILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFS 120
           ++MA+SAR NDE + ESPAKALRRILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFS
Sbjct: 61  QIMAISARNNDEIIAESPAKALRRILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFS 120

Query: 121 ISAARLALPDTGYMSYGEMLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGF 180
           ISAARLALPDTG++SYGEMLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGF
Sbjct: 121 ISAARLALPDTGFISYGEMLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGF 180

Query: 181 AGIILEDQVSPKACGHTQGRKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSL 240
           AGIILEDQVSPKACGHTQGRKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSL
Sbjct: 181 AGIILEDQVSPKACGHTQGRKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSL 240

Query: 241 SESLRRARAFADAGADVLFIDALASKDEMDAFCKISPKVPKMANMLEGGGKTPILNPLEL 300
           SESL RARAFADAGADVLFIDALASKDEM+AFCKISPK+PKMANMLEGGGKTPI NPLEL
Sbjct: 241 SESLWRARAFADAGADVLFIDALASKDEMEAFCKISPKIPKMANMLEGGGKTPIFNPLEL 300

Query: 301 EEMGFKIVAYPLSLVGVSIRAMQDALLAIKGGRLPSPGTLPTFAEMKELLGFNSYYEEER 360
           EE+G+K+VAYPLSLVGVSIRAMQDALLAIKGGRLPSPGTLPTF EMKE+LGFNSYYEEER
Sbjct: 301 EEIGYKLVAYPLSLVGVSIRAMQDALLAIKGGRLPSPGTLPTFGEMKEILGFNSYYEEER 360

Query: 361 KYASAVSQPSIKVDSSITSLQRRVEDDKE-KGQSSQGPAVEVITPEIYRSYDDDGSRGPF 420
           KYASAVSQPSIKVDS+I+SLQRRVEDD E K Q+ QGP VEVITPEIYRSYD D S+GPF
Sbjct: 361 KYASAVSQPSIKVDSNISSLQRRVEDDSEKKDQNPQGPVVEVITPEIYRSYDADESKGPF 420

Query: 421 SGIWSRRLRVKITGRDGFERLDVRIPAGFLEGLTNIVPALGGVNIKELMDDAAGEVGGKQ 480
           SGIWSRRLRVKITGRDGFERLDVRIPAGFLEGLTNIVPALGGVNIKELMDDAAGEVGGK 
Sbjct: 421 SGIWSRRLRVKITGRDGFERLDVRIPAGFLEGLTNIVPALGGVNIKELMDDAAGEVGGKL 480

Query: 481 LLDFVDGMGDRIEVFLE 497
           LLDFVDGMGDRIEVFLE
Sbjct: 481 LLDFVDGMGDRIEVFLE 497

BLAST of Chy7G142540 vs. NCBI nr
Match: XP_004152091.1 (uncharacterized protein LOC101208914 [Cucumis sativus] >KGN53115.1 hypothetical protein Csa_015112 [Cucumis sativus])

HSP 1 Score: 944 bits (2440), Expect = 0.0
Identity = 485/496 (97.78%), Postives = 489/496 (98.59%), Query Frame = 0

Query: 1   MKLPVASKDGSLSLPLSHTESLRLQLPSPPPHSFPLSLSWRPSIKRNISLSNNPHHKPLS 60
           MKLPVASKDGSL+LPLSHT SLRLQLPS PPHS P SLSWRPS+K NISLSNNP+HKPLS
Sbjct: 1   MKLPVASKDGSLTLPLSHTASLRLQLPSLPPHSIPRSLSWRPSVKGNISLSNNPYHKPLS 60

Query: 61  RVMAMSARINDEYVVESPAKALRRILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFS 120
           RVMAMSARINDE VVESPAKALRRILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFS
Sbjct: 61  RVMAMSARINDESVVESPAKALRRILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFS 120

Query: 121 ISAARLALPDTGYMSYGEMLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGF 180
           ISAARLALPDTGYMSYGEMLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGF
Sbjct: 121 ISAARLALPDTGYMSYGEMLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGF 180

Query: 181 AGIILEDQVSPKACGHTQGRKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSL 240
           AGIILEDQVSPKACGHTQGRKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSL
Sbjct: 181 AGIILEDQVSPKACGHTQGRKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSL 240

Query: 241 SESLRRARAFADAGADVLFIDALASKDEMDAFCKISPKVPKMANMLEGGGKTPILNPLEL 300
           SESLRRARAFADAGADVLFIDALASKDEM+AFCKISPKVPKMANMLEGGGKTPILNPLEL
Sbjct: 241 SESLRRARAFADAGADVLFIDALASKDEMEAFCKISPKVPKMANMLEGGGKTPILNPLEL 300

Query: 301 EEMGFKIVAYPLSLVGVSIRAMQDALLAIKGGRLPSPGTLPTFAEMKELLGFNSYYEEER 360
           EEMGFKIVAYPLSLVGVSIRAMQDALLAIKGGRLPSPGTLPTFAEMKELLGFNSYYEEER
Sbjct: 301 EEMGFKIVAYPLSLVGVSIRAMQDALLAIKGGRLPSPGTLPTFAEMKELLGFNSYYEEER 360

Query: 361 KYASAVSQPSIKVDSSITSLQRRVEDDKEKGQSSQGPAVEVITPEIYRSYDDDGSRGPFS 420
           KYASAVSQPSIKVDSSITSLQRRVEDDKEKGQ SQGPAVEVITPEIYRSYDDDGSRGPFS
Sbjct: 361 KYASAVSQPSIKVDSSITSLQRRVEDDKEKGQGSQGPAVEVITPEIYRSYDDDGSRGPFS 420

Query: 421 GIWSRRLRVKITGRDGFERLDVRIPAGFLEGLTNIVPALGGVNIKELMDDAAGEVGGKQL 480
           GIWSRRLRVKITGRDGFERLDVRIPAGFLEGLTNIVPALGGVNIKELMDDAAGEVGGKQL
Sbjct: 421 GIWSRRLRVKITGRDGFERLDVRIPAGFLEGLTNIVPALGGVNIKELMDDAAGEVGGKQL 480

Query: 481 LDFVDGMGDRIEVFLE 496
           LDFVDGMGDRIEVFLE
Sbjct: 481 LDFVDGMGDRIEVFLE 496

BLAST of Chy7G142540 vs. NCBI nr
Match: XP_008453941.1 (PREDICTED: 2,3-dimethylmalate lyase [Cucumis melo] >XP_008453942.1 PREDICTED: 2,3-dimethylmalate lyase [Cucumis melo])

HSP 1 Score: 932 bits (2408), Expect = 0.0
Identity = 480/497 (96.58%), Postives = 487/497 (97.99%), Query Frame = 0

Query: 1   MKLPVASKDGSLSLPLSHTESLRLQLPSPPPHSFPLSLSWRPSIKRNISLSNNPHHKPLS 60
           MKLPVASKDGSLSLPLSH  SLRL LPSPPPHS P SLSWRPSIKRNISLSNNPHHKPLS
Sbjct: 1   MKLPVASKDGSLSLPLSHIASLRLHLPSPPPHSLPRSLSWRPSIKRNISLSNNPHHKPLS 60

Query: 61  RVMAMSARINDEYVVESPAKALRRILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFS 120
           RVMAMSARINDE +VESPAKALRRILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFS
Sbjct: 61  RVMAMSARINDESIVESPAKALRRILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFS 120

Query: 121 ISAARLALPDTGYMSYGEMLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGF 180
           ISAARLALPDTGY+SYGEMLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGF
Sbjct: 121 ISAARLALPDTGYISYGEMLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGF 180

Query: 181 AGIILEDQVSPKACGHTQGRKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSL 240
           AGIILEDQVSPKACGHTQGRKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSL
Sbjct: 181 AGIILEDQVSPKACGHTQGRKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSL 240

Query: 241 SESLRRARAFADAGADVLFIDALASKDEMDAFCKISPKVPKMANMLEGGGKTPILNPLEL 300
           SESLRRARAFADAGADVLFIDALASKDEM+AFCKISPKVPKMANMLEGGGKTPIL+PLEL
Sbjct: 241 SESLRRARAFADAGADVLFIDALASKDEMEAFCKISPKVPKMANMLEGGGKTPILSPLEL 300

Query: 301 EEMGFKIVAYPLSLVGVSIRAMQDALLAIKGGRLPSPGTLPTFAEMKELLGFNSYYEEER 360
           EEMGFKIVAYPLSLVGVSIRAMQDALLAIKGGRLPSPGTLPTFAEMKELLGFNSYYEEER
Sbjct: 301 EEMGFKIVAYPLSLVGVSIRAMQDALLAIKGGRLPSPGTLPTFAEMKELLGFNSYYEEER 360

Query: 361 KYASAVSQPSIKVDSSITSLQRRVEDDKEK-GQSSQGPAVEVITPEIYRSYDDDGSRGPF 420
           KYASAVSQPSIKVDSSITSLQR+VED+KEK GQS  GPAVEVITPEI RSYDDDGSRGPF
Sbjct: 361 KYASAVSQPSIKVDSSITSLQRKVEDNKEKKGQSPPGPAVEVITPEIVRSYDDDGSRGPF 420

Query: 421 SGIWSRRLRVKITGRDGFERLDVRIPAGFLEGLTNIVPALGGVNIKELMDDAAGEVGGKQ 480
           SGIWSRRLRVKITGRDGFERLDVRIPAGFL+GLTNIVPALGGVNIKELMDDAAGEVGGKQ
Sbjct: 421 SGIWSRRLRVKITGRDGFERLDVRIPAGFLDGLTNIVPALGGVNIKELMDDAAGEVGGKQ 480

Query: 481 LLDFVDGMGDRIEVFLE 496
           LLDFVDGMGDRIEVFLE
Sbjct: 481 LLDFVDGMGDRIEVFLE 497

BLAST of Chy7G142540 vs. NCBI nr
Match: TYK16962.1 (2,3-dimethylmalate lyase [Cucumis melo var. makuwa])

HSP 1 Score: 918 bits (2373), Expect = 0.0
Identity = 476/497 (95.77%), Postives = 483/497 (97.18%), Query Frame = 0

Query: 1   MKLPVASKDGSLSLPLSHTESLRLQLPSPPPHSFPLSLSWRPSIKRNISLSNNPHHKPLS 60
           MKLPVASKDGSLSLPLSH  SLRL LPSPPPHS P SLSWRPSIKRNISLSNNPHHKPLS
Sbjct: 1   MKLPVASKDGSLSLPLSHIASLRLHLPSPPPHSLPRSLSWRPSIKRNISLSNNPHHKPLS 60

Query: 61  RVMAMSARINDEYVVESPAKALRRILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFS 120
           RVMAMSARINDE +VESPAKALRRILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFS
Sbjct: 61  RVMAMSARINDESIVESPAKALRRILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFS 120

Query: 121 ISAARLALPDTGYMSYGEMLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGF 180
           ISAARLALPDTGY+SYGEMLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGF
Sbjct: 121 ISAARLALPDTGYISYGEMLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGF 180

Query: 181 AGIILEDQVSPKACGHTQGRKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSL 240
           AGIILEDQVSPKACGHTQGRKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSL
Sbjct: 181 AGIILEDQVSPKACGHTQGRKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSL 240

Query: 241 SESLRRARAFADAGADVLFIDALASKDEMDAFCKISPKVPKMANMLEGGGKTPILNPLEL 300
           SESLRRARAFADAGADVLFIDALASKDEM+AFCKISPKVPKMANMLEGGGKTPIL+PLEL
Sbjct: 241 SESLRRARAFADAGADVLFIDALASKDEMEAFCKISPKVPKMANMLEGGGKTPILSPLEL 300

Query: 301 EEMGFKIVAYPLSLVGVSIRAMQDALLAIKGGRLPSPGTLPTFAEMKELLGFNSYYEEER 360
           EEMGFKIVAYPLSLVGVSIRAMQDALLAIKGGRLPSPGTLPTFAEMKELLGFNSYYEEER
Sbjct: 301 EEMGFKIVAYPLSLVGVSIRAMQDALLAIKGGRLPSPGTLPTFAEMKELLGFNSYYEEER 360

Query: 361 KYASAVSQPSIKVDSSITSLQRRVEDDKEK-GQSSQGPAVEVITPEIYRSYDDDGSRGPF 420
           KYASAVSQPSIKVDSSITSLQR+VED+KEK GQS  GPAVEVITPEI RSYDDDGSRGPF
Sbjct: 361 KYASAVSQPSIKVDSSITSLQRKVEDNKEKKGQSPPGPAVEVITPEIVRSYDDDGSRGPF 420

Query: 421 SGIWSRRLRVKITGRDGFERLDVRIPAGFLEGLTNIVPALGGVNIKELMDDAAGEVGGKQ 480
           SGIWSRRLRVKITGRDGFERLD    AGFL+GLTNIVPALGGVNIKELMDDAAGEVGGKQ
Sbjct: 421 SGIWSRRLRVKITGRDGFERLD----AGFLDGLTNIVPALGGVNIKELMDDAAGEVGGKQ 480

Query: 481 LLDFVDGMGDRIEVFLE 496
           LLDFVDGMGDRIEVFLE
Sbjct: 481 LLDFVDGMGDRIEVFLE 493

BLAST of Chy7G142540 vs. NCBI nr
Match: XP_038905100.1 (2,3-dimethylmalate lyase isoform X2 [Benincasa hispida])

HSP 1 Score: 887 bits (2293), Expect = 0.0
Identity = 456/497 (91.75%), Postives = 470/497 (94.57%), Query Frame = 0

Query: 1   MKLPVASKDGSLSLPLSHTESLRLQLPSPPPHSFPLSLSWRPSIKRNISLSNNPHHKPLS 60
           MKLPVAS DGSLSL LSH+ SLRLQLPSPPPHS   SLSWRPSIKR ISL  NP  KP+S
Sbjct: 1   MKLPVASNDGSLSLSLSHSASLRLQLPSPPPHSLRQSLSWRPSIKRTISLFTNPQQKPVS 60

Query: 61  RVMAMSARINDEYVVESPAKALRRILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFS 120
            VMAMSARINDE  VESPAKALR+ILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFS
Sbjct: 61  LVMAMSARINDESAVESPAKALRKILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFS 120

Query: 121 ISAARLALPDTGYMSYGEMLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGF 180
           ISAARLALPDTGY+SYGEMLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGF
Sbjct: 121 ISAARLALPDTGYISYGEMLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGF 180

Query: 181 AGIILEDQVSPKACGHTQGRKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSL 240
           AGIILEDQVSPKACGHTQGRKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSL
Sbjct: 181 AGIILEDQVSPKACGHTQGRKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSL 240

Query: 241 SESLRRARAFADAGADVLFIDALASKDEMDAFCKISPKVPKMANMLEGGGKTPILNPLEL 300
           SESLRRARAFAD GADVLFIDALASKDEM+AFCKISPK+PKMANMLEGGGKTPILNPLEL
Sbjct: 241 SESLRRARAFADVGADVLFIDALASKDEMEAFCKISPKIPKMANMLEGGGKTPILNPLEL 300

Query: 301 EEMGFKIVAYPLSLVGVSIRAMQDALLAIKGGRLPSPGTLPTFAEMKELLGFNSYYEEER 360
           EE+GFK+V YPLSLVGVSIRAMQDALLAIKGGRLPSPGTLPTF EMKE+LGFNSYYEEER
Sbjct: 301 EEVGFKLVVYPLSLVGVSIRAMQDALLAIKGGRLPSPGTLPTFGEMKEILGFNSYYEEER 360

Query: 361 KYASAVSQPSIKVDSSITSLQRRVEDDKEK-GQSSQGPAVEVITPEIYRSYDDDGSRGPF 420
           KYASAVSQPSIKV SSI SLQRRVEDD EK  Q+ Q P VEVITPEIYR+YD DGS+GPF
Sbjct: 361 KYASAVSQPSIKVGSSINSLQRRVEDDSEKKDQNPQSPVVEVITPEIYRNYDGDGSKGPF 420

Query: 421 SGIWSRRLRVKITGRDGFERLDVRIPAGFLEGLTNIVPALGGVNIKELMDDAAGEVGGKQ 480
           SGIWSRRLRVKITGRDGFERLDVRIPAGFLEGLTNIVPALGG+NIKELMDDAAGEVGGK 
Sbjct: 421 SGIWSRRLRVKITGRDGFERLDVRIPAGFLEGLTNIVPALGGINIKELMDDAAGEVGGKL 480

Query: 481 LLDFVDGMGDRIEVFLE 496
           LLDF+DGMGDRIEVFLE
Sbjct: 481 LLDFIDGMGDRIEVFLE 497

BLAST of Chy7G142540 vs. NCBI nr
Match: XP_038905074.1 (2,3-dimethylmalate lyase isoform X1 [Benincasa hispida] >XP_038905080.1 2,3-dimethylmalate lyase isoform X1 [Benincasa hispida] >XP_038905090.1 2,3-dimethylmalate lyase isoform X1 [Benincasa hispida])

HSP 1 Score: 882 bits (2279), Expect = 0.0
Identity = 456/500 (91.20%), Postives = 470/500 (94.00%), Query Frame = 0

Query: 1   MKLPVASKDGSLSLPLSHTESLRLQLPSPPPHSFPLSLSWRPSIKRNISLSNNPHHKPLS 60
           MKLPVAS DGSLSL LSH+ SLRLQLPSPPPHS   SLSWRPSIKR ISL  NP  KP+S
Sbjct: 1   MKLPVASNDGSLSLSLSHSASLRLQLPSPPPHSLRQSLSWRPSIKRTISLFTNPQQKPVS 60

Query: 61  RVMAMSARINDEYVVESPAKALRRILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFS 120
            VMAMSARINDE  VESPAKALR+ILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFS
Sbjct: 61  LVMAMSARINDESAVESPAKALRKILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFS 120

Query: 121 ISAARLALPDTGYMSYGEMLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGF 180
           ISAARLALPDTGY+SYGEMLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGF
Sbjct: 121 ISAARLALPDTGYISYGEMLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGF 180

Query: 181 AGIILEDQVSPKACGHTQGRKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSL 240
           AGIILEDQVSPKACGHTQGRKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSL
Sbjct: 181 AGIILEDQVSPKACGHTQGRKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSL 240

Query: 241 SESLRRARAFADAGADVLFIDALASKDEMDAFCKISPKVPKM---ANMLEGGGKTPILNP 300
           SESLRRARAFAD GADVLFIDALASKDEM+AFCKISPK+PKM   ANMLEGGGKTPILNP
Sbjct: 241 SESLRRARAFADVGADVLFIDALASKDEMEAFCKISPKIPKMSLKANMLEGGGKTPILNP 300

Query: 301 LELEEMGFKIVAYPLSLVGVSIRAMQDALLAIKGGRLPSPGTLPTFAEMKELLGFNSYYE 360
           LELEE+GFK+V YPLSLVGVSIRAMQDALLAIKGGRLPSPGTLPTF EMKE+LGFNSYYE
Sbjct: 301 LELEEVGFKLVVYPLSLVGVSIRAMQDALLAIKGGRLPSPGTLPTFGEMKEILGFNSYYE 360

Query: 361 EERKYASAVSQPSIKVDSSITSLQRRVEDDKEK-GQSSQGPAVEVITPEIYRSYDDDGSR 420
           EERKYASAVSQPSIKV SSI SLQRRVEDD EK  Q+ Q P VEVITPEIYR+YD DGS+
Sbjct: 361 EERKYASAVSQPSIKVGSSINSLQRRVEDDSEKKDQNPQSPVVEVITPEIYRNYDGDGSK 420

Query: 421 GPFSGIWSRRLRVKITGRDGFERLDVRIPAGFLEGLTNIVPALGGVNIKELMDDAAGEVG 480
           GPFSGIWSRRLRVKITGRDGFERLDVRIPAGFLEGLTNIVPALGG+NIKELMDDAAGEVG
Sbjct: 421 GPFSGIWSRRLRVKITGRDGFERLDVRIPAGFLEGLTNIVPALGGINIKELMDDAAGEVG 480

Query: 481 GKQLLDFVDGMGDRIEVFLE 496
           GK LLDF+DGMGDRIEVFLE
Sbjct: 481 GKLLLDFIDGMGDRIEVFLE 500

BLAST of Chy7G142540 vs. TAIR 10
Match: AT2G43180.1 (Phosphoenolpyruvate carboxylase family protein )

HSP 1 Score: 560.5 bits (1443), Expect = 1.4e-159
Identity = 291/421 (69.12%), Postives = 345/421 (81.95%), Query Frame = 0

Query: 77  SPAKALRRILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFSISAARLALPDTGYMSY 136
           SPAK LR I++ PGV QGP CFDALSAKL+ERAGF YC TSGFSISA+RL LPD G +SY
Sbjct: 71  SPAKKLRNIMQSPGVLQGPCCFDALSAKLIERAGFPYCITSGFSISASRLGLPDKGLISY 130

Query: 137 GEMLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGFAGIILEDQVSPKACGH 196
           GEM+DQGQ ITQSVSIPVIGDG NGYGNAMNVKRTVKGYI+AGFAGII+ D+V    C +
Sbjct: 131 GEMVDQGQQITQSVSIPVIGDGGNGYGNAMNVKRTVKGYIKAGFAGIIINDEV---CCEN 190

Query: 197 TQG-RKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSLSESLRRARAFADAGA 256
           T+  R+VVSREEAV+R+KAAVDAR+E  SDIVIVA+TDSR+A+SL ESL RARAF DAGA
Sbjct: 191 TKSERRVVSREEAVMRVKAAVDARRECDSDIVIVAQTDSREAISLEESLIRARAFTDAGA 250

Query: 257 DVLFIDALASKDEMDAFCKISPKVPKMANMLEGGGKTPILNPLELEEMGFKIVAYPLSLV 316
           DVL +D+L S++EM AFC + P VPK+ANMLE GGK PILNPLELEE+G+K+VAYP+SL+
Sbjct: 251 DVLSVDSLVSREEMKAFCNVYPLVPKLANMLESGGKIPILNPLELEEIGYKLVAYPISLI 310

Query: 317 GVSIRAMQDALLAIKGGRLPSPGTLPTFAEMKELLGFNSYYEEERKYASAVSQPSIKVDS 376
           GVSI+AMQDALLAIKGGR+P PG++ +  E+ E+LGF++Y EEE++YA++ S   +   S
Sbjct: 311 GVSIQAMQDALLAIKGGRIPPPGSMASLEEIGEILGFDTYEEEEKRYATSSSDREVS-SS 370

Query: 377 SITSLQRRVEDDKEKGQSSQGPAVEVITPEIYRSYDDDGSRGPFSGIWSRRLRVKITGRD 436
           S+   QR  +DD E+ +      VEVITPE+Y        R PFS IWSR LR+KI GRD
Sbjct: 371 SVYRNQRVAKDDPEQREDL---IVEVITPEVYNE-----PRNPFSRIWSRSLRIKIIGRD 430

Query: 437 GFERLDVRIPAGFLEGLTNIVPALGGVNIKELMDDAAGEVGGKQLLDFVDGMGDRIEVFL 496
           GFE+LDVRIPAGFLEG+TNIVPALGGVN+K+LMDDAA EVGGK LLDF D  GDRI+VFL
Sbjct: 431 GFEKLDVRIPAGFLEGVTNIVPALGGVNLKQLMDDAADEVGGKILLDFKDTAGDRIQVFL 479

BLAST of Chy7G142540 vs. TAIR 10
Match: AT2G43180.3 (Phosphoenolpyruvate carboxylase family protein )

HSP 1 Score: 557.4 bits (1435), Expect = 1.2e-158
Identity = 291/421 (69.12%), Postives = 344/421 (81.71%), Query Frame = 0

Query: 77  SPAKALRRILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFSISAARLALPDTGYMSY 136
           SPAK LR I++ PGV QGP CFDALSAKL+ERAGF YC TSGFSISA+RL LPD G +SY
Sbjct: 71  SPAKKLRNIMQSPGVLQGPCCFDALSAKLIERAGFPYCITSGFSISASRLGLPDKGLISY 130

Query: 137 GEMLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGFAGIILEDQVSPKACGH 196
           GEM+DQGQ ITQSVSIPVIGDG NGYGNAMNVKRTVKGYI+AGFAGII+ D+V    C +
Sbjct: 131 GEMVDQGQQITQSVSIPVIGDGGNGYGNAMNVKRTVKGYIKAGFAGIIINDEV---CCEN 190

Query: 197 TQG-RKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSLSESLRRARAFADAGA 256
           T+  R+VVSREEAV+R+KAAVDAR+E  SDIVIVA+TDSR+A+SL ESL RARAF DAGA
Sbjct: 191 TKSERRVVSREEAVMRVKAAVDARRECDSDIVIVAQTDSREAISLEESLIRARAFTDAGA 250

Query: 257 DVLFIDALASKDEMDAFCKISPKVPKMANMLEGGGKTPILNPLELEEMGFKIVAYPLSLV 316
           DVL +D+L S++EM AFC + P VPK+ANMLE GGK PILNPLELEE+G+K+VAYP+SL+
Sbjct: 251 DVLSVDSLVSREEMKAFCNVYPLVPKLANMLESGGKIPILNPLELEEIGYKLVAYPISLI 310

Query: 317 GVSIRAMQDALLAIKGGRLPSPGTLPTFAEMKELLGFNSYYEEERKYASAVSQPSIKVDS 376
           GVSI+AMQDALLAIKGGR+P PG++ +  E+ E+LGF++Y EEE++YA++ S       S
Sbjct: 311 GVSIQAMQDALLAIKGGRIPPPGSMASLEEIGEILGFDTYEEEEKRYATSSSDR--VSSS 370

Query: 377 SITSLQRRVEDDKEKGQSSQGPAVEVITPEIYRSYDDDGSRGPFSGIWSRRLRVKITGRD 436
           S+   QR  +DD E+ +      VEVITPE+Y        R PFS IWSR LR+KI GRD
Sbjct: 371 SVYRNQRVAKDDPEQREDL---IVEVITPEVYNE-----PRNPFSRIWSRSLRIKIIGRD 430

Query: 437 GFERLDVRIPAGFLEGLTNIVPALGGVNIKELMDDAAGEVGGKQLLDFVDGMGDRIEVFL 496
           GFE+LDVRIPAGFLEG+TNIVPALGGVN+K+LMDDAA EVGGK LLDF D  GDRI+VFL
Sbjct: 431 GFEKLDVRIPAGFLEGVTNIVPALGGVNLKQLMDDAADEVGGKILLDFKDTAGDRIQVFL 478

BLAST of Chy7G142540 vs. TAIR 10
Match: AT2G43180.2 (Phosphoenolpyruvate carboxylase family protein )

HSP 1 Score: 501.9 bits (1291), Expect = 5.9e-142
Identity = 260/384 (67.71%), Postives = 312/384 (81.25%), Query Frame = 0

Query: 77  SPAKALRRILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFSISAARLALPDTGYMSY 136
           SPAK LR I++ PGV QGP CFDALSAKL+ERAGF YC TSGFSISA+RL LPD G +SY
Sbjct: 71  SPAKKLRNIMQSPGVLQGPCCFDALSAKLIERAGFPYCITSGFSISASRLGLPDKGLISY 130

Query: 137 GEMLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGFAGIILEDQVSPKACGH 196
           GEM+DQGQ ITQSVSIPVIGDG NGYGNAMNVKRTVKGYI+AGFAGII+ D+V    C +
Sbjct: 131 GEMVDQGQQITQSVSIPVIGDGGNGYGNAMNVKRTVKGYIKAGFAGIIINDEV---CCEN 190

Query: 197 TQG-RKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSLSESLRRARAFADAGA 256
           T+  R+VVSREEAV+R+KAAVDAR+E  SDIVIVA+TDSR+A+SL ESL RARAF DAGA
Sbjct: 191 TKSERRVVSREEAVMRVKAAVDARRECDSDIVIVAQTDSREAISLEESLIRARAFTDAGA 250

Query: 257 DVLFIDALASKDEMDAFCKISPKVPKMANMLEGGGKTPILNPLELEEMGFKIVAYPLSLV 316
           DVL +D+L S++EM AFC + P VPK+ANMLE GGK PILNPLELEE+G+K+VAYP+SL+
Sbjct: 251 DVLSVDSLVSREEMKAFCNVYPLVPKLANMLESGGKIPILNPLELEEIGYKLVAYPISLI 310

Query: 317 GVSIRAMQDALLAIKGGRLPSPGTLPTFAEMKELLGFNSYYEEERKYASAVSQPSIKVDS 376
           GVSI+AMQDALLAIKGGR+P PG++ +  E+ E+LGF++Y EEE++YA++ S   +   S
Sbjct: 311 GVSIQAMQDALLAIKGGRIPPPGSMASLEEIGEILGFDTYEEEEKRYATSSSDREVS-SS 370

Query: 377 SITSLQRRVEDDKEKGQSSQGPAVEVITPEIYRSYDDDGSRGPFSGIWSRRLRVKITGRD 436
           S+   QR  +DD E+ +      VEVITPE+Y        R PFS IWSR LR+KI GRD
Sbjct: 371 SVYRNQRVAKDDPEQREDL---IVEVITPEVYNE-----PRNPFSRIWSRSLRIKIIGRD 430

Query: 437 GFERLDVRIPAGFLEGLTNIVPAL 460
           GFE+LDVRIPAGFLEG+TNIVP +
Sbjct: 431 GFEKLDVRIPAGFLEGVTNIVPVI 442

BLAST of Chy7G142540 vs. TAIR 10
Match: AT2G43180.4 (Phosphoenolpyruvate carboxylase family protein )

HSP 1 Score: 478.0 bits (1229), Expect = 9.1e-135
Identity = 249/370 (67.30%), Postives = 299/370 (80.81%), Query Frame = 0

Query: 77  SPAKALRRILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFSISAARLALPDTGYMSY 136
           SPAK LR I++ PGV QGP CFDALSAKL+ERAGF YC TSGFSISA+RL LPD G +SY
Sbjct: 71  SPAKKLRNIMQSPGVLQGPCCFDALSAKLIERAGFPYCITSGFSISASRLGLPDKGLISY 130

Query: 137 GEMLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGFAGIILEDQVSPKACGH 196
           GEM+DQGQ ITQSVSIPVIGDG NGYGNAMNVKRTVKGYI+AGFAGII+ D+V    C +
Sbjct: 131 GEMVDQGQQITQSVSIPVIGDGGNGYGNAMNVKRTVKGYIKAGFAGIIINDEV---CCEN 190

Query: 197 TQG-RKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSLSESLRRARAFADAGA 256
           T+  R+VVSREEAV+R+KAAVDAR+E  SDIVIVA+TDSR+A+SL ESL RARAF DAGA
Sbjct: 191 TKSERRVVSREEAVMRVKAAVDARRECDSDIVIVAQTDSREAISLEESLIRARAFTDAGA 250

Query: 257 DVLFIDALASKDEMDAFCKISPKVPKMANMLEGGGKTPILNPLELEEMGFKIVAYPLSLV 316
           DVL +D+L S++EM AFC + P VPK+ANMLE GGK PILNPLELEE+G+K+VAYP+SL+
Sbjct: 251 DVLSVDSLVSREEMKAFCNVYPLVPKLANMLESGGKIPILNPLELEEIGYKLVAYPISLI 310

Query: 317 GVSIRAMQDALLAIKGGRLPSPGTLPTFAEMKELLGFNSYYEEERKYASAVSQPSIKVDS 376
           GVSI+AMQDALLAIKGGR+P PG++ +  E+ E+LGF++Y EEE++YA++ S   +   S
Sbjct: 311 GVSIQAMQDALLAIKGGRIPPPGSMASLEEIGEILGFDTYEEEEKRYATSSSDREVS-SS 370

Query: 377 SITSLQRRVEDDKEKGQSSQGPAVEVITPEIYRSYDDDGSRGPFSGIWSRRLRVKITGRD 436
           S+   QR  +DD E+ +      VEVITPE+Y        R PFS IWSR LR+KI GRD
Sbjct: 371 SVYRNQRVAKDDPEQREDL---IVEVITPEVYNE-----PRNPFSRIWSRSLRIKIIGRD 428

Query: 437 GFERLDVRIP 446
           GFE+LDVRIP
Sbjct: 431 GFEKLDVRIP 428

BLAST of Chy7G142540 vs. TAIR 10
Match: AT1G77060.1 (Phosphoenolpyruvate carboxylase family protein )

HSP 1 Score: 188.3 bits (477), Expect = 1.4e-47
Identity = 121/335 (36.12%), Postives = 187/335 (55.82%), Query Frame = 0

Query: 36  LSLSWRPSIKRNISLSNNPHHKPLSRVMAMSARINDEYVVESPAKALRRILEMPGVHQGP 95
           L+L+  P+ +RN            +R++  +ARI   +          R++E  G+   P
Sbjct: 17  LNLTASPTFRRN---------PRAARLVNPTARIQTRF---------HRLIEEQGIVLMP 76

Query: 96  ACFDALSAKLVERAGFLYCFTSGFSISAARLALPDTGYMSYGEMLDQGQLITQSV-SIPV 155
            C+DALSA +V++ GF   F SG+++SA+ L  PD G ++  EM    + +  S  +IP+
Sbjct: 77  GCYDALSAAIVQQTGFSAGFISGYALSASLLGKPDFGLLTPPEMAATARSVCASAPNIPI 136

Query: 156 IGDGDNGYGNAMNVKRTVKGYIRAGFAGIILEDQVSPKACGHTQGRKVVSREEAVLRIKA 215
           I D D G GNA+N++RTVK  I AG AG  LEDQ  PK CGH +G++V+  EE   +I +
Sbjct: 137 IADADTGGGNALNIQRTVKDLIAAGAAGCFLEDQAWPKKCGHMRGKQVIPAEEHAAKIAS 196

Query: 216 AVDARKESGSDIVIVARTDSRQAVS---LSESLRRARAFADAGADVLFIDALASKDEMDA 275
           A DA  +  SD  +VARTD R   +   L +++ R   + +AGAD  F++A    DE+  
Sbjct: 197 ARDAIGD--SDFFLVARTDVRATSAKSGLEDAIARVNLYMEAGADASFVEAPRDDDELKE 256

Query: 276 FCKISPKVPKMANMLEGGGKTPILNPLELEEMGFKIVAYPLSLVGVSIRAMQDALLAIK- 335
             K   K  ++ NM+E GG TP+  P EL+EMGF ++ +PL+ +  S RA+ D L  +K 
Sbjct: 257 IGK-RTKGYRVCNMIE-GGVTPLHTPDELKEMGFHLIVHPLTALYASTRALVDVLKTLKE 316

Query: 336 -GGRLPSPGTLPTFAEMKELLGFNSYYEEERKYAS 365
            G        + TF E   L+  +S++E E +Y++
Sbjct: 317 NGSTRDHLQKMATFEEFNSLVDLDSWFELEARYSN 329