Chy7G141060 (gene) Cucumber (hystrix) v1

Overview


Name	Chy7G141060
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	homeobox-leucine zipper protein ATHB-40
Location	chrH07: 17481412 .. 17483594 (+)
RNA-Seq Expression	Chy7G141060
Synteny	Chy7G141060

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTACCCCAACAAGGTTTGTACCCTTCTTTTTTGTTAAAGGCTTTTTGCCTCTTTTTGGTAAAATTTACTCAAATACTCTTTCTTTTTTCCTCTTTTCTTCTGTGAATTGAACCTCATTTTTTTAAGCATTTTAGGTCATTTGGGCGAGTCTAAACCGAGACGGCGTCGTAAGAAGAGCAGAGGATCGGAAACAGAGGCGGCGTCGGGTGGGAAAAAGAGGAAGCTGACGGCGGCGCAAGTTCATCTTTTGGAGTCGAATTTTGGTAGTGAACATAAATTGGAATCGGAAAGAAAGGACCGGTTAGCGTCGGAGTTGGGGTTGGATCCCCGGCAGGTGGCTGTGTGGTTTCAGAACCGCCGTGCACGGTGGAAGAATAAGAAACTTGAGGAGGAGTATTCTAATTTGAAGAAGCTTCATGAATCCGTCGTCGTTGAAAAATGCCGTCTTGAAACAGAGGTTTGTTTCCCTTTCTCCTTTATCTTCTTTCATCCTTAATTGAATCTAAACCATTTTCTTTATTGTTATCTAATTTAATTAGTTTAAGGGAGAAAATGGGAGAAAACTTCTAACACTATGATCAATATTTAAGATTTGGTATCATTTAATAATAATGTATTAGTCAATTGATTAGTTTTCAAAACAATACATTATTCAATTATATATTCAGTTCAAATTAATTAACCATACATACTAAACTAAAAAGTAAAAAGTAAAAAAAAAAAAAAAAAGTTACATAGTCAAACTGATTTTGCTTCATCGAAAGTTTGTTTGATCTTTCTTTTTAGAAACTTTCAATTTTCCATTCCTGCATGTGTTGTTAAAAAGTTATGTGTACACTACTCAAGACTAAGTGAACATTACTTAAGATTAGACATATATCTTAAACACTAATTACCCTTTAACATTTGGTACCTACACAAGATATCTCTACTCATTTTTACATTCGAACCTCTTACTTACCATAATCTTTCCATTTCTATATAAATCCCTAAATTTCAGGAACCTTGCATCAAAGTCAAAGGAGAGACGCAAAATTGAAAACACAAAAACCTAGAAAAAGTTTAACTAATTTTTTTTATAATTGAAGCAGTTAGTTGCAAAATCAAAAGTGAAATTTGTAAATGTAGCAAAATACAAAGAAATTGCAAATAGTTTCGCTGATCACTCAAAAATATTGGATAGTCTAAAGATTAATTTGGGTAAAAATAAAGGGTGGAAACAAAGAAAAAGAAAATAAAGAGTGGAGGAGAAAAGGTGGGTAACTTTGGGTGAAAAAGAGAGGAGTGGACCAACATGAATGAACGTGCATGGGCTCTTGGCTCGACGAAACCCCCACCCCACTTTGTTTTACAATCAGATGGCAAATATATATATATATCTATCTATCTATATACTTATATATTTATTTTCTTTTAGTAAAGAGAAAAGAAAATGGTTTGACAGAACTTTTGATAAGCCAATTGCCTTACAACACCCACCACCACCTCATTCCTAAACTATATTATTTTCTAAAATTTTAAATTACAAAGTGAGTAATAAAGTTATTTCGTACTATGTTGTCTCTGTTTCTATGGGCCATCGCCCCACTTTGGTGCCTCTCACTTCTCACTCTAACATTTCACTTATCAACACTAAATTCTATCTTCCACTTACTTCGTACTACAACACTACACCATTATGTAAATGTGAAAGAAAATATTCACTTTAGTAGCTATTTTGTTTTTCTTTTTCTTTTAATATCTTGTTTTGTTTTTGATATCTACTTTCCACCCATACTTTCAAAACAAAGTCAAAAATGTTGTTTTTAAAAACTTTTTTGTTTTCATATTTGAAAATAGTACGTTTGGGTTTAATTTTGTCATAATATTTTTTTTGTACCATATGAAAATAGAATTAGCTACTAAAGAATATTTTTCAAAATAAAACGATGCAGCTATTGAAGCTAAAAGAGCAACTATTAGAAGTAGAGAAAGAGAAAGAGAGGGTAAGAATGGGAGAAGGGGGTGATGGGCCATTAAGTAGTAGTAGCCCAAGCCCATCAATGTCAATGGAAGCTATGGAGATGGAGCAACCCATATCCCTTGGGGAAATTTCACCATTATATGAAGAAGATGTGTTTTATTACATGCCTGAAAATTATTGTATTTATGGCATGGAATGGCCTAATCCCTACCTTTGA

mRNA sequence

ATGCTACCCCAACAAGGTCATTTGGGCGAGTCTAAACCGAGACGGCGTCGTAAGAAGAGCAGAGGATCGGAAACAGAGGCGGCGTCGGGTGGGAAAAAGAGGAAGCTGACGGCGGCGCAAGTTCATCTTTTGGAGTCGAATTTTGGTAGTGAACATAAATTGGAATCGGAAAGAAAGGACCGGTTAGCGTCGGAGTTGGGGTTGGATCCCCGGCAGGTGGCTGTGTGGTTTCAGAACCGCCGTGCACGGTGGAAGAATAAGAAACTTGAGGAGGAGTATTCTAATTTGAAGAAGCTTCATGAATCCGTCGTCGTTGAAAAATGCCGTCTTGAAACAGAGCTATTGAAGCTAAAAGAGCAACTATTAGAAGTAGAGAAAGAGAAAGAGAGGGTAAGAATGGGAGAAGGGGGTGATGGGCCATTAAGTAGTAGTAGCCCAAGCCCATCAATGTCAATGGAAGCTATGGAGATGGAGCAACCCATATCCCTTGGGGAAATTTCACCATTATATGAAGAAGATGTGTTTTATTACATGCCTGAAAATTATTGTATTTATGGCATGGAATGGCCTAATCCCTACCTTTGA

Coding sequence (CDS)

ATGCTACCCCAACAAGGTCATTTGGGCGAGTCTAAACCGAGACGGCGTCGTAAGAAGAGCAGAGGATCGGAAACAGAGGCGGCGTCGGGTGGGAAAAAGAGGAAGCTGACGGCGGCGCAAGTTCATCTTTTGGAGTCGAATTTTGGTAGTGAACATAAATTGGAATCGGAAAGAAAGGACCGGTTAGCGTCGGAGTTGGGGTTGGATCCCCGGCAGGTGGCTGTGTGGTTTCAGAACCGCCGTGCACGGTGGAAGAATAAGAAACTTGAGGAGGAGTATTCTAATTTGAAGAAGCTTCATGAATCCGTCGTCGTTGAAAAATGCCGTCTTGAAACAGAGCTATTGAAGCTAAAAGAGCAACTATTAGAAGTAGAGAAAGAGAAAGAGAGGGTAAGAATGGGAGAAGGGGGTGATGGGCCATTAAGTAGTAGTAGCCCAAGCCCATCAATGTCAATGGAAGCTATGGAGATGGAGCAACCCATATCCCTTGGGGAAATTTCACCATTATATGAAGAAGATGTGTTTTATTACATGCCTGAAAATTATTGTATTTATGGCATGGAATGGCCTAATCCCTACCTTTGA

Protein sequence

MLPQQGHLGESKPRRRRKKSRGSETEAASGGKKRKLTAAQVHLLESNFGSEHKLESERKDRLASELGLDPRQVAVWFQNRRARWKNKKLEEEYSNLKKLHESVVVEKCRLETELLKLKEQLLEVEKEKERVRMGEGGDGPLSSSSPSPSMSMEAMEMEQPISLGEISPLYEEDVFYYMPENYCIYGMEWPNPYL*

Homology

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BLAST of Chy7G141060 vs. ExPASy Swiss-Prot
Match: O23208 (Homeobox-leucine zipper protein ATHB-40 OS=Arabidopsis thaliana OX=3702 GN=ATHB-40 PE=2 SV=3)

HSP 1 Score: 170.6 bits (431), Expect = 1.7e-41
Identity = 106/192 (55.21%), Postives = 138/192 (71.88%), Query Frame = 0


Query: 9   GESK-PRRRRKKSRGSETEAASGG---KKRKLTAAQVHLLESNFGSEHKLESERKDRLAS 68
           GE K P+RRRKK++GS   A  G    +KRKLT  QV++LE +FG EHKLESERKDRLA+
Sbjct: 28  GEVKQPKRRRKKTKGSVASADGGNGLFRKRKLTDEQVNMLEMSFGDEHKLESERKDRLAA 87

Query: 69  ELGLDPRQVAVWFQNRRARWKNKKLEEEYSNLKKLHESVVVEKCRLETELLKLKEQLLEV 128
           ELGLDPRQVAVWFQNRRARWKNK+LEEEY+ LK  H++VVV+KCRLE+E+++LKEQL + 
Sbjct: 88  ELGLDPRQVAVWFQNRRARWKNKRLEEEYNKLKNSHDNVVVDKCRLESEVIQLKEQLYDA 147

Query: 129 EKEKERVRMGEGGDGPLSSSSPSPSMSMEAMEME--QPISLGEISPLYEEDVFYYMPENY 188
           E+E +  R+ E  +G  S+S  S S+S+EA E        +G+    Y+  +FY +PEN 
Sbjct: 148 EREIQ--RLAERVEGGSSNSPISSSVSVEANETPFFGDYKVGDDGDDYDH-LFYPVPENS 207

Query: 189 CIYGMEWPNPYL 195
            I   EW + Y+
Sbjct: 208 YIDEAEWMSLYI 216

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BLAST of Chy7G141060 vs. ExPASy Swiss-Prot
Match: Q9ZU70 (Homeobox-leucine zipper protein ATHB-21 OS=Arabidopsis thaliana OX=3702 GN=ATHB-21 PE=2 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 6.3e-36
Identity = 91/161 (56.52%), Postives = 119/161 (73.91%), Query Frame = 0


Query: 1   MLPQQGHLGESKPRRRRKKSRGS-------ETEAASGGKKRKLTAAQVHLLESNFGSEHK 60
           ++PQQG  GE+KP RRRK+   S       E E     +KRKL+  QV +LE +F  +HK
Sbjct: 25  LVPQQG--GEAKPTRRRKRKSKSVVVAEEGENEGNGWFRKRKLSDEQVRMLEISFEDDHK 84

Query: 61  LESERKDRLASELGLDPRQVAVWFQNRRARWKNKKLEEEYSNLKKLHESVVVEKCRLETE 120
           LESERKDRLASELGLDPRQVAVWFQNRRARWKNK++E+EY+ LK  +E+ VVEKCRL++E
Sbjct: 85  LESERKDRLASELGLDPRQVAVWFQNRRARWKNKRVEDEYTKLKNAYETTVVEKCRLDSE 144

Query: 121 LLKLKEQLLEVEKEKERVRMGEGGDGPLSSSSPSPSMSMEA 155
           ++ LKEQL E E+E +  R+ +  +G LS+S  S S+++EA
Sbjct: 145 VIHLKEQLYEAEREIQ--RLAKRVEGTLSNSPISSSVTIEA 181

[Close]

BLAST of Chy7G141060 vs. ExPASy Swiss-Prot
Match: Q9LVR0 (Homeobox-leucine zipper protein ATHB-53 OS=Arabidopsis thaliana OX=3702 GN=ATHB-53 PE=2 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 1.0e-33
Identity = 96/197 (48.73%), Postives = 134/197 (68.02%), Query Frame = 0


Query: 10  ESKP--RRRRKKSRGS-----ETEAASGG--KKRKLTAAQVHLLESNFGSEHKLESERKD 69
           ESKP  RRR+++S+GS     E  A  GG  +KRKLT  QV++LE +FG+EHKLES RK+
Sbjct: 40  ESKPVKRRRKRRSKGSSATNEEDVAEIGGMLRKRKLTDEQVNMLEYSFGNEHKLESGRKE 99

Query: 70  RLASELGLDPRQVAVWFQNRRARWKNKKLEEEYSNLKKLHESVVVEKCRLETELLKLKEQ 129
           ++A ELGLDPRQVAVWFQNRRARWKNKKLEEEY+ LK  H++VV+ +C+LE+++LKL EQ
Sbjct: 100 KIAGELGLDPRQVAVWFQNRRARWKNKKLEEEYAKLKNHHDNVVLGQCQLESQILKLTEQ 159

Query: 130 LLEVEKEKERV--RMGEGGDGPLSSSSPSPSMSMEAMEMEQPISLGEISPLYEEDVFYYM 189
           L E + E  ++  R+ E     + ++S S S+S+EA          E++P    ++ +YM
Sbjct: 160 LSEAQSEIRKLSERLEE-----MPTNSSSSSLSVEANNAPTDF---ELAPETNYNIPFYM 219

Query: 190 PENYCIYGME-WPNPYL 195
            +N  +  ME W   Y+
Sbjct: 220 LDNNYLQSMEYWDGLYV 228

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BLAST of Chy7G141060 vs. ExPASy Swiss-Prot
Match: Q7XI85 (Homeobox-leucine zipper protein HOX14 OS=Oryza sativa subsp. japonica OX=39947 GN=HOX14 PE=2 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 4.4e-29
Identity = 89/204 (43.63%), Postives = 118/204 (57.84%), Query Frame = 0


Query: 1   MLPQQGHLGESKPRRRRKKSRGSETEAASGG---------KKRKLTAAQVHLLESNFGSE 60
           +L   G +   +PR RR++ RG+      GG         KKR+L+  QV +LE +F  E
Sbjct: 22  LLAASGEVQGERPRARRRRRRGARCVGGGGGGGEVDGGDPKKRRLSDEQVEMLELSFREE 81

Query: 61  HKLESERKDRLASELGLDPRQVAVWFQNRRARWKNKKLEEEYSNLKKLHESVVVEKCRLE 120
            KLE+ RK  LASELGLDP+QVAVWFQNRRAR K+K LEEE+S LK  H++ ++ KC LE
Sbjct: 82  RKLETGRKVHLASELGLDPKQVAVWFQNRRARHKSKLLEEEFSKLKHAHDAAILHKCHLE 141

Query: 121 TELLKLKEQLLEVEKEKERVR---------------MGEGGDGPLSSSSPSPSMSMEAME 180
            E+L+LKE+L+  E+E  R+R               MG GG G   + SPS S S     
Sbjct: 142 NEVLRLKERLVVAEEEVRRLRSAAGSHTASGEGGDIMGLGGSGACVAGSPSSSFSTGTC- 201

[Close]

BLAST of Chy7G141060 vs. ExPASy Swiss-Prot
Match: A2YN17 (Homeobox-leucine zipper protein HOX14 OS=Oryza sativa subsp. indica OX=39946 GN=HOX14 PE=2 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 4.9e-28
Identity = 84/188 (44.68%), Postives = 109/188 (57.98%), Query Frame = 0


Query: 1   MLPQQGHLGESKPRRRRKKSRGSETEAASGG---------KKRKLTAAQVHLLESNFGSE 60
           +L   G +   +PR RR++ RG+      GG         KKR+L+  QV +LE +F  E
Sbjct: 22  LLAASGEVQGERPRARRRRRRGARCVGGGGGGGEVDGGDPKKRRLSDEQVEMLELSFREE 81

Query: 61  HKLESERKDRLASELGLDPRQVAVWFQNRRARWKNKKLEEEYSNLKKLHESVVVEKCRLE 120
            KLE+ RK  LASELGLDP+QVAVWFQNRRAR K+K LEEE+S LK  H++ ++ KC LE
Sbjct: 82  RKLETGRKVHLASELGLDPKQVAVWFQNRRARHKSKLLEEEFSKLKHAHDAAILHKCHLE 141

Query: 121 TELLKLKEQLLEVEKEKERVR---------------MGEGGDGPLSSSSPSPSMSMEAME 165
            E+L+LKE+L+  E+E  R+R               MG GG G   + SPS S S     
Sbjct: 142 NEVLRLKERLVVAEEEVRRLRSAAGSHTASGEGGDIMGLGGSGACVAGSPSSSFSTGTC- 201

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BLAST of Chy7G141060 vs. ExPASy TrEMBL
Match: A0A0A0KWL3 (Homeobox domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G043850 PE=4 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 2.7e-98
Identity = 191/194 (98.45%), Postives = 192/194 (98.97%), Query Frame = 0


Query: 1   MLPQQGHLGESKPRRRRKKSRGSETEAASGGKKRKLTAAQVHLLESNFGSEHKLESERKD 60
           MLPQQ HLGESKPRRRRKK+RGSETEAASGGKKRKLTAAQV LLESNFGSEHKLESERKD
Sbjct: 26  MLPQQVHLGESKPRRRRKKNRGSETEAASGGKKRKLTAAQVRLLESNFGSEHKLESERKD 85

Query: 61  RLASELGLDPRQVAVWFQNRRARWKNKKLEEEYSNLKKLHESVVVEKCRLETELLKLKEQ 120
           RLASELGLDPRQVAVWFQNRRARWKNKKLEEEYSNLKKLHESVVVEKCRLETELLKLKEQ
Sbjct: 86  RLASELGLDPRQVAVWFQNRRARWKNKKLEEEYSNLKKLHESVVVEKCRLETELLKLKEQ 145

Query: 121 LLEVEKEKERVRMGEGGDGPLSSSSPSPSMSMEAMEMEQPISLGEISPLYEEDVFYYMPE 180
           LLEVEKEKERVRMGEGGDGPLSSSSPSPSMSMEAMEMEQPISLGEISPLYEEDVFYYMPE
Sbjct: 146 LLEVEKEKERVRMGEGGDGPLSSSSPSPSMSMEAMEMEQPISLGEISPLYEEDVFYYMPE 205

Query: 181 NYCIYGMEWPNPYL 195
           NYCIYGMEWPNPYL
Sbjct: 206 NYCIYGMEWPNPYL 219

[Close]

BLAST of Chy7G141060 vs. ExPASy TrEMBL
Match: A0A1S3BY52 (homeobox-leucine zipper protein ATHB-40 OS=Cucumis melo OX=3656 GN=LOC103494370 PE=4 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 5.3e-94
Identity = 185/194 (95.36%), Postives = 190/194 (97.94%), Query Frame = 0


Query: 1   MLPQQGHLGESKPRRRRKKSRGSETEAASGGKKRKLTAAQVHLLESNFGSEHKLESERKD 60
           MLPQQG+LGESKPRRRRKK+RGSETEA+SGGKKRKLTAAQVHLLESNFGSEHKLESERKD
Sbjct: 25  MLPQQGNLGESKPRRRRKKNRGSETEASSGGKKRKLTAAQVHLLESNFGSEHKLESERKD 84

Query: 61  RLASELGLDPRQVAVWFQNRRARWKNKKLEEEYSNLKKLHESVVVEKCRLETELLKLKEQ 120
           RLASELGLDPRQVAVWFQNRRARWKNKKLEEEYSNLKKLHESVVVEKCRLETELLKLKEQ
Sbjct: 85  RLASELGLDPRQVAVWFQNRRARWKNKKLEEEYSNLKKLHESVVVEKCRLETELLKLKEQ 144

Query: 121 LLEVEKEKERVRMGEGGDGPLSSSSPSPSMSMEAMEMEQPISLGEISPLYEEDVFYYMPE 180
           L+EVEKEKERV MGE  DGPLSSSSPSPSMSMEAMEM+ PISLGEISPLYEEDVFYYMPE
Sbjct: 145 LIEVEKEKERVIMGERADGPLSSSSPSPSMSMEAMEMD-PISLGEISPLYEEDVFYYMPE 204

Query: 181 NYCIYGMEWPNPYL 195
           NYCIYGMEWPNPYL
Sbjct: 205 NYCIYGMEWPNPYL 217

[Close]

BLAST of Chy7G141060 vs. ExPASy TrEMBL
Match: A0A6J1FTB2 (homeobox-leucine zipper protein ATHB-40-like OS=Cucurbita moschata OX=3662 GN=LOC111447938 PE=4 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 2.1e-74
Identity = 157/196 (80.10%), Postives = 173/196 (88.27%), Query Frame = 0


Query: 1   MLPQQ--GHLGESKPRRRRKKSRGSETEAASGGKKRKLTAAQVHLLESNFGSEHKLESER 60
           M+PQQ   +LGE+K RRRRKK+RGSE +A +  KKRKLTAAQVHLLE+NFGSEHKLESER
Sbjct: 25  MVPQQQDNNLGEAKRRRRRKKNRGSEADAVA--KKRKLTAAQVHLLETNFGSEHKLESER 84

Query: 61  KDRLASELGLDPRQVAVWFQNRRARWKNKKLEEEYSNLKKLHESVVVEKCRLETELLKLK 120
           KDRLASELGLDPRQVAVWFQNRRARWKNKKLEEEYS+LKKLHESVVVEKCRLET++LKLK
Sbjct: 85  KDRLASELGLDPRQVAVWFQNRRARWKNKKLEEEYSSLKKLHESVVVEKCRLETQILKLK 144

Query: 121 EQLLEVEKEKERVRMGEGGDGPLSSSSPSPSMSMEAMEMEQPISLGEISPLYEEDVFYYM 180
           EQL E EKEKER+ MGE GDGPLSSSSPSPSMSM+A++   P  LGE    + EDVFYYM
Sbjct: 145 EQLSEAEKEKERLIMGERGDGPLSSSSPSPSMSMDAVD---PTGLGEFGASF-EDVFYYM 204

Query: 181 PENYCIYGMEWPNPYL 195
           PEN CIYGMEWPNPY+
Sbjct: 205 PENSCIYGMEWPNPYI 214

[Close]

BLAST of Chy7G141060 vs. ExPASy TrEMBL
Match: A0A6J1J034 (homeobox-leucine zipper protein ATHB-40-like OS=Cucurbita maxima OX=3661 GN=LOC111481488 PE=4 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 3.0e-73
Identity = 156/196 (79.59%), Postives = 172/196 (87.76%), Query Frame = 0


Query: 1   MLPQQ--GHLGESKPRRRRKKSRGSETEAASGGKKRKLTAAQVHLLESNFGSEHKLESER 60
           M+PQQ   +LGE+K RRRRKK+RGSE +A +  KKRKLTAAQVHLLE++FGSEHKLESER
Sbjct: 25  MVPQQQDNNLGEAKRRRRRKKNRGSEADAVA--KKRKLTAAQVHLLETHFGSEHKLESER 84

Query: 61  KDRLASELGLDPRQVAVWFQNRRARWKNKKLEEEYSNLKKLHESVVVEKCRLETELLKLK 120
           KDRLASELGLDPRQVAVWFQNRRARWKNKKLEEEYS+LKKLHESVVVEKCRLET++LKLK
Sbjct: 85  KDRLASELGLDPRQVAVWFQNRRARWKNKKLEEEYSSLKKLHESVVVEKCRLETQILKLK 144

Query: 121 EQLLEVEKEKERVRMGEGGDGPLSSSSPSPSMSMEAMEMEQPISLGEISPLYEEDVFYYM 180
           EQL EVEKEKER  MGE GDGPLSSSSPSPSMSM+A++   P  L E    + EDVFYYM
Sbjct: 145 EQLSEVEKEKERQTMGERGDGPLSSSSPSPSMSMDAVD---PTGLEEFGASF-EDVFYYM 204

Query: 181 PENYCIYGMEWPNPYL 195
           PEN CIYGMEWPNPY+
Sbjct: 205 PENSCIYGMEWPNPYI 214

[Close]

BLAST of Chy7G141060 vs. ExPASy TrEMBL
Match: A0A6J1C5L6 (homeobox-leucine zipper protein ATHB-40-like OS=Momordica charantia OX=3673 GN=LOC111008181 PE=4 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 8.5e-68
Identity = 155/202 (76.73%), Postives = 166/202 (82.18%), Query Frame = 0


Query: 1   MLPQQGHLGESKPRRRRKKSRG-SETE-AASGGKKRKLTAAQVHLLESNFGSEHKLESER 60
           M PQQG+ GESKPRRRRKK+RG SE+E AA+  KKRKLTA QVHLLE+NFGSEHKLESER
Sbjct: 25  MAPQQGNSGESKPRRRRKKNRGSSESEAAAAAAKKRKLTAQQVHLLETNFGSEHKLESER 84

Query: 61  KDRLASELGLDPRQVAVWFQNRRARWKNKKLEEEYSNLKKLHESVVVEKCRLETELLKLK 120
           KDRLASELGLDPRQVAVWFQNRRARWKNKKLEEEYS+LKKLHESVVVEKCRLETE+L LK
Sbjct: 85  KDRLASELGLDPRQVAVWFQNRRARWKNKKLEEEYSHLKKLHESVVVEKCRLETEILNLK 144

Query: 121 EQLLEVEKEKERVRMGEGGDGPLSSSSPSPSMSMEAMEMEQPISLGEISPLYEEDVFYYM 180
           EQL E EKEKER+      DGPL SSSPSPSMSMEA++      LGE      EDVFYYM
Sbjct: 145 EQLSEAEKEKERLMR---ADGPL-SSSPSPSMSMEAVDPTSYSCLGEFGSYELEDVFYYM 204

Query: 181 PE----NYCIYG--MEWPNPYL 195
           PE    N CIYG  MEWPNPY+
Sbjct: 205 PEAESDNTCIYGMEMEWPNPYI 222

[Close]

BLAST of Chy7G141060 vs. NCBI nr
Match: XP_004146482.1 (homeobox-leucine zipper protein ATHB-40 [Cucumis sativus] >KGN53269.1 hypothetical protein Csa_014985 [Cucumis sativus])

HSP 1 Score: 368 bits (945), Expect = 9.36e-128
Identity = 191/194 (98.45%), Postives = 192/194 (98.97%), Query Frame = 0


Query: 1   MLPQQGHLGESKPRRRRKKSRGSETEAASGGKKRKLTAAQVHLLESNFGSEHKLESERKD 60
           MLPQQ HLGESKPRRRRKK+RGSETEAASGGKKRKLTAAQV LLESNFGSEHKLESERKD
Sbjct: 26  MLPQQVHLGESKPRRRRKKNRGSETEAASGGKKRKLTAAQVRLLESNFGSEHKLESERKD 85

Query: 61  RLASELGLDPRQVAVWFQNRRARWKNKKLEEEYSNLKKLHESVVVEKCRLETELLKLKEQ 120
           RLASELGLDPRQVAVWFQNRRARWKNKKLEEEYSNLKKLHESVVVEKCRLETELLKLKEQ
Sbjct: 86  RLASELGLDPRQVAVWFQNRRARWKNKKLEEEYSNLKKLHESVVVEKCRLETELLKLKEQ 145

Query: 121 LLEVEKEKERVRMGEGGDGPLSSSSPSPSMSMEAMEMEQPISLGEISPLYEEDVFYYMPE 180
           LLEVEKEKERVRMGEGGDGPLSSSSPSPSMSMEAMEMEQPISLGEISPLYEEDVFYYMPE
Sbjct: 146 LLEVEKEKERVRMGEGGDGPLSSSSPSPSMSMEAMEMEQPISLGEISPLYEEDVFYYMPE 205

Query: 181 NYCIYGMEWPNPYL 194
           NYCIYGMEWPNPYL
Sbjct: 206 NYCIYGMEWPNPYL 219

[Close]

BLAST of Chy7G141060 vs. NCBI nr
Match: XP_008453727.1 (PREDICTED: homeobox-leucine zipper protein ATHB-40 [Cucumis melo])

HSP 1 Score: 354 bits (908), Expect = 3.80e-122
Identity = 185/194 (95.36%), Postives = 190/194 (97.94%), Query Frame = 0


Query: 1   MLPQQGHLGESKPRRRRKKSRGSETEAASGGKKRKLTAAQVHLLESNFGSEHKLESERKD 60
           MLPQQG+LGESKPRRRRKK+RGSETEA+SGGKKRKLTAAQVHLLESNFGSEHKLESERKD
Sbjct: 25  MLPQQGNLGESKPRRRRKKNRGSETEASSGGKKRKLTAAQVHLLESNFGSEHKLESERKD 84

Query: 61  RLASELGLDPRQVAVWFQNRRARWKNKKLEEEYSNLKKLHESVVVEKCRLETELLKLKEQ 120
           RLASELGLDPRQVAVWFQNRRARWKNKKLEEEYSNLKKLHESVVVEKCRLETELLKLKEQ
Sbjct: 85  RLASELGLDPRQVAVWFQNRRARWKNKKLEEEYSNLKKLHESVVVEKCRLETELLKLKEQ 144

Query: 121 LLEVEKEKERVRMGEGGDGPLSSSSPSPSMSMEAMEMEQPISLGEISPLYEEDVFYYMPE 180
           L+EVEKEKERV MGE  DGPLSSSSPSPSMSMEAMEM+ PISLGEISPLYEEDVFYYMPE
Sbjct: 145 LIEVEKEKERVIMGERADGPLSSSSPSPSMSMEAMEMD-PISLGEISPLYEEDVFYYMPE 204

Query: 181 NYCIYGMEWPNPYL 194
           NYCIYGMEWPNPYL
Sbjct: 205 NYCIYGMEWPNPYL 217

[Close]

BLAST of Chy7G141060 vs. NCBI nr
Match: XP_038877287.1 (homeobox-leucine zipper protein ATHB-40-like [Benincasa hispida])

HSP 1 Score: 320 bits (820), Expect = 8.73e-109
Identity = 170/194 (87.63%), Postives = 179/194 (92.27%), Query Frame = 0


Query: 1   MLPQQGHLGESKPRRRRKKSRGSETEAASGGKKRKLTAAQVHLLESNFGSEHKLESERKD 60
           M+PQQG+L ESKPRRRRKK+RGSE EAA+GGKKRKLTAAQV LLESNFGSEHKLESERKD
Sbjct: 25  MVPQQGNLSESKPRRRRKKNRGSEAEAAAGGKKRKLTAAQVLLLESNFGSEHKLESERKD 84

Query: 61  RLASELGLDPRQVAVWFQNRRARWKNKKLEEEYSNLKKLHESVVVEKCRLETELLKLKEQ 120
           RLASELGLDPRQVAVWFQNRRARWKNKKLEEEYSNLKKLHESVVVEKCRLETE+LKLKEQ
Sbjct: 85  RLASELGLDPRQVAVWFQNRRARWKNKKLEEEYSNLKKLHESVVVEKCRLETEILKLKEQ 144

Query: 121 LLEVEKEKERVRMGEGGDGPLSSSSPSPSMSMEAMEMEQPISLGEISPLYEEDVFYYMPE 180
           L E EKEKERV MGE  DGPLSSSSPSPSMSM+A++   P  LGEI PLYE DVFYYMPE
Sbjct: 145 LSEAEKEKERVIMGERADGPLSSSSPSPSMSMDAVD---PTCLGEIPPLYE-DVFYYMPE 204

Query: 181 NYCIYGMEWPNPYL 194
           NYCIYGMEWPNPY+
Sbjct: 205 NYCIYGMEWPNPYI 214

[Close]

BLAST of Chy7G141060 vs. NCBI nr
Match: KAG6600601.1 (Homeobox-leucine zipper protein ATHB-40, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 290 bits (741), Expect = 4.10e-97
Identity = 158/196 (80.61%), Postives = 174/196 (88.78%), Query Frame = 0


Query: 1   MLPQQ--GHLGESKPRRRRKKSRGSETEAASGGKKRKLTAAQVHLLESNFGSEHKLESER 60
           M+PQQ   +LGE+K RRRRKK+RGSE +A +  KKRKLTAAQVHLLE+NFGSEHKLESER
Sbjct: 1   MVPQQQDNNLGEAKRRRRRKKNRGSEADAVA--KKRKLTAAQVHLLETNFGSEHKLESER 60

Query: 61  KDRLASELGLDPRQVAVWFQNRRARWKNKKLEEEYSNLKKLHESVVVEKCRLETELLKLK 120
           KDRLASELGLDPRQVAVWFQNRRARWKNKKLEEEYS+LKKLHESVVVEKCRLET++LKLK
Sbjct: 61  KDRLASELGLDPRQVAVWFQNRRARWKNKKLEEEYSSLKKLHESVVVEKCRLETQILKLK 120

Query: 121 EQLLEVEKEKERVRMGEGGDGPLSSSSPSPSMSMEAMEMEQPISLGEISPLYEEDVFYYM 180
           EQL EVEKEKER+ MGE GDGPLSSSSPSPSMSM+A++   P  LGE    +E DVFYYM
Sbjct: 121 EQLSEVEKEKERLIMGERGDGPLSSSSPSPSMSMDAVD---PTGLGEFGASFE-DVFYYM 180

Query: 181 PENYCIYGMEWPNPYL 194
           PEN CIYGMEWPNPY+
Sbjct: 181 PENSCIYGMEWPNPYI 190

[Close]

BLAST of Chy7G141060 vs. NCBI nr
Match: KAG7031239.1 (Homeobox-leucine zipper protein ATHB-40 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 290 bits (741), Expect = 9.34e-97
Identity = 158/196 (80.61%), Postives = 174/196 (88.78%), Query Frame = 0


Query: 1   MLPQQ--GHLGESKPRRRRKKSRGSETEAASGGKKRKLTAAQVHLLESNFGSEHKLESER 60
           M+PQQ   +LGE+K RRRRKK+RGSE +A +  KKRKLTAAQVHLLE+NFGSEHKLESER
Sbjct: 25  MVPQQQDNNLGEAKRRRRRKKNRGSEADAVA--KKRKLTAAQVHLLETNFGSEHKLESER 84

Query: 61  KDRLASELGLDPRQVAVWFQNRRARWKNKKLEEEYSNLKKLHESVVVEKCRLETELLKLK 120
           KDRLASELGLDPRQVAVWFQNRRARWKNKKLEEEYS+LKKLHESVVVEKCRLET++LKLK
Sbjct: 85  KDRLASELGLDPRQVAVWFQNRRARWKNKKLEEEYSSLKKLHESVVVEKCRLETQILKLK 144

Query: 121 EQLLEVEKEKERVRMGEGGDGPLSSSSPSPSMSMEAMEMEQPISLGEISPLYEEDVFYYM 180
           EQL EVEKEKER+ MGE GDGPLSSSSPSPSMSM+A++   P  LGE    +E DVFYYM
Sbjct: 145 EQLSEVEKEKERLIMGERGDGPLSSSSPSPSMSMDAVD---PTGLGEFGASFE-DVFYYM 204

Query: 181 PENYCIYGMEWPNPYL 194
           PEN CIYGMEWPNPY+
Sbjct: 205 PENSCIYGMEWPNPYI 214

[Close]

BLAST of Chy7G141060 vs. TAIR 10
Match: AT4G36740.1 (homeobox protein 40 )

HSP 1 Score: 170.6 bits (431), Expect = 1.2e-42
Identity = 106/192 (55.21%), Postives = 138/192 (71.88%), Query Frame = 0


Query: 9   GESK-PRRRRKKSRGSETEAASGG---KKRKLTAAQVHLLESNFGSEHKLESERKDRLAS 68
           GE K P+RRRKK++GS   A  G    +KRKLT  QV++LE +FG EHKLESERKDRLA+
Sbjct: 28  GEVKQPKRRRKKTKGSVASADGGNGLFRKRKLTDEQVNMLEMSFGDEHKLESERKDRLAA 87

Query: 69  ELGLDPRQVAVWFQNRRARWKNKKLEEEYSNLKKLHESVVVEKCRLETELLKLKEQLLEV 128
           ELGLDPRQVAVWFQNRRARWKNK+LEEEY+ LK  H++VVV+KCRLE+E+++LKEQL + 
Sbjct: 88  ELGLDPRQVAVWFQNRRARWKNKRLEEEYNKLKNSHDNVVVDKCRLESEVIQLKEQLYDA 147

Query: 129 EKEKERVRMGEGGDGPLSSSSPSPSMSMEAMEME--QPISLGEISPLYEEDVFYYMPENY 188
           E+E +  R+ E  +G  S+S  S S+S+EA E        +G+    Y+  +FY +PEN 
Sbjct: 148 EREIQ--RLAERVEGGSSNSPISSSVSVEANETPFFGDYKVGDDGDDYDH-LFYPVPENS 207

Query: 189 CIYGMEWPNPYL 195
            I   EW + Y+
Sbjct: 208 YIDEAEWMSLYI 216

[Close]

BLAST of Chy7G141060 vs. TAIR 10
Match: AT2G18550.1 (homeobox protein 21 )

HSP 1 Score: 152.1 bits (383), Expect = 4.5e-37
Identity = 91/161 (56.52%), Postives = 119/161 (73.91%), Query Frame = 0


Query: 1   MLPQQGHLGESKPRRRRKKSRGS-------ETEAASGGKKRKLTAAQVHLLESNFGSEHK 60
           ++PQQG  GE+KP RRRK+   S       E E     +KRKL+  QV +LE +F  +HK
Sbjct: 25  LVPQQG--GEAKPTRRRKRKSKSVVVAEEGENEGNGWFRKRKLSDEQVRMLEISFEDDHK 84

Query: 61  LESERKDRLASELGLDPRQVAVWFQNRRARWKNKKLEEEYSNLKKLHESVVVEKCRLETE 120
           LESERKDRLASELGLDPRQVAVWFQNRRARWKNK++E+EY+ LK  +E+ VVEKCRL++E
Sbjct: 85  LESERKDRLASELGLDPRQVAVWFQNRRARWKNKRVEDEYTKLKNAYETTVVEKCRLDSE 144

Query: 121 LLKLKEQLLEVEKEKERVRMGEGGDGPLSSSSPSPSMSMEA 155
           ++ LKEQL E E+E +  R+ +  +G LS+S  S S+++EA
Sbjct: 145 VIHLKEQLYEAEREIQ--RLAKRVEGTLSNSPISSSVTIEA 181

[Close]

BLAST of Chy7G141060 vs. TAIR 10
Match: AT5G66700.1 (homeobox 53 )

HSP 1 Score: 144.8 bits (364), Expect = 7.2e-35
Identity = 96/197 (48.73%), Postives = 134/197 (68.02%), Query Frame = 0


Query: 10  ESKP--RRRRKKSRGS-----ETEAASGG--KKRKLTAAQVHLLESNFGSEHKLESERKD 69
           ESKP  RRR+++S+GS     E  A  GG  +KRKLT  QV++LE +FG+EHKLES RK+
Sbjct: 40  ESKPVKRRRKRRSKGSSATNEEDVAEIGGMLRKRKLTDEQVNMLEYSFGNEHKLESGRKE 99

Query: 70  RLASELGLDPRQVAVWFQNRRARWKNKKLEEEYSNLKKLHESVVVEKCRLETELLKLKEQ 129
           ++A ELGLDPRQVAVWFQNRRARWKNKKLEEEY+ LK  H++VV+ +C+LE+++LKL EQ
Sbjct: 100 KIAGELGLDPRQVAVWFQNRRARWKNKKLEEEYAKLKNHHDNVVLGQCQLESQILKLTEQ 159

Query: 130 LLEVEKEKERV--RMGEGGDGPLSSSSPSPSMSMEAMEMEQPISLGEISPLYEEDVFYYM 189
           L E + E  ++  R+ E     + ++S S S+S+EA          E++P    ++ +YM
Sbjct: 160 LSEAQSEIRKLSERLEE-----MPTNSSSSSLSVEANNAPTDF---ELAPETNYNIPFYM 219

Query: 190 PENYCIYGME-WPNPYL 195
            +N  +  ME W   Y+
Sbjct: 220 LDNNYLQSMEYWDGLYV 228

[Close]

BLAST of Chy7G141060 vs. TAIR 10
Match: AT3G01470.1 (homeobox 1 )

HSP 1 Score: 95.5 bits (236), Expect = 5.0e-20
Identity = 61/149 (40.94%), Postives = 88/149 (59.06%), Query Frame = 0


Query: 32  KKRKLTAAQVHLLESNFGSEHKLESERKDRLASELGLDPRQVAVWFQNRRARWKNKKLEE 91
           KKR+LT  QVHLLE +F +E+KLE ERK +LA +LGL PRQVAVWFQNRRARWK K+LE 
Sbjct: 68  KKRRLTTEQVHLLEKSFETENKLEPERKTQLAKKLGLQPRQVAVWFQNRRARWKTKQLER 127

Query: 92  EYSNLKKL-------HESVVVEKCRLETELLKLKEQLLEVEKEKERVRMGEGGDGPLSSS 151
           +Y  LK         ++S+V++  +L +E+  L E+L    + K+       G  P  + 
Sbjct: 128 DYDLLKSTYDQLLSNYDSIVMDNDKLRSEVTSLTEKL----QGKQETANEPPGQVPEPNQ 187

Query: 152 SPSPSMSMEAMEMEQPISLGEISPLYEED 174
                ++  A++ E  +S G +     +D
Sbjct: 188 LDPVYINAAAIKTEDRLSSGSVGSAVLDD 212

[Close]

BLAST of Chy7G141060 vs. TAIR 10
Match: AT1G69780.1 (Homeobox-leucine zipper protein family )

HSP 1 Score: 89.7 bits (221), Expect = 2.7e-18
Identity = 54/129 (41.86%), Postives = 81/129 (62.79%), Query Frame = 0


Query: 23  SETEAASGGKKRKLTAAQVHLLESNFGSEHKLESERKDRLASELGLDPRQVAVWFQNRRA 82
           S+  +  G KKR+L   QV  LE NF   +KLE ERK +LA  LGL PRQ+A+WFQNRRA
Sbjct: 76  SDDGSQMGEKKRRLNMEQVKTLEKNFELGNKLEPERKMQLARALGLQPRQIAIWFQNRRA 135

Query: 83  RWKNKKLEEEYSNLKKLHESVVVEKCRLETELLKLKEQL--LEVEKEKERVRMGEGGDGP 142
           RWK K+LE++Y  LK+  +++  E   L+T   KL+ ++  L+  ++ E + + +  +G 
Sbjct: 136 RWKTKQLEKDYDTLKRQFDTLKAENDLLQTHNQKLQAEIMGLKNREQTESINLNKETEGS 195

Query: 143 LSSSSPSPS 150
            S+ S + S
Sbjct: 196 CSNRSDNSS 204

The following BLAST results are available for this feature:

BLAST of Chy7G141060 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23208	1.7e-41	55.21	Homeobox-leucine zipper protein ATHB-40 OS=Arabidopsis thaliana OX=3702 GN=ATHB-...	[more]
Q9ZU70	6.3e-36	56.52	Homeobox-leucine zipper protein ATHB-21 OS=Arabidopsis thaliana OX=3702 GN=ATHB-...	[more]
Q9LVR0	1.0e-33	48.73	Homeobox-leucine zipper protein ATHB-53 OS=Arabidopsis thaliana OX=3702 GN=ATHB-...	[more]
Q7XI85	4.4e-29	43.63	Homeobox-leucine zipper protein HOX14 OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
A2YN17	4.9e-28	44.68	Homeobox-leucine zipper protein HOX14 OS=Oryza sativa subsp. indica OX=39946 GN=...	[more]

BLAST of Chy7G141060 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KWL3	2.7e-98	98.45	Homeobox domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G043850 PE...	[more]
A0A1S3BY52	5.3e-94	95.36	homeobox-leucine zipper protein ATHB-40 OS=Cucumis melo OX=3656 GN=LOC103494370 ...	[more]
A0A6J1FTB2	2.1e-74	80.10	homeobox-leucine zipper protein ATHB-40-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1J034	3.0e-73	79.59	homeobox-leucine zipper protein ATHB-40-like OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1C5L6	8.5e-68	76.73	homeobox-leucine zipper protein ATHB-40-like OS=Momordica charantia OX=3673 GN=L...	[more]

BLAST of Chy7G141060 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004146482.1	9.36e-128	98.45	homeobox-leucine zipper protein ATHB-40 [Cucumis sativus] >KGN53269.1 hypothetic...	[more]
XP_008453727.1	3.80e-122	95.36	PREDICTED: homeobox-leucine zipper protein ATHB-40 [Cucumis melo]	[more]
XP_038877287.1	8.73e-109	87.63	homeobox-leucine zipper protein ATHB-40-like [Benincasa hispida]	[more]
KAG6600601.1	4.10e-97	80.61	Homeobox-leucine zipper protein ATHB-40, partial [Cucurbita argyrosperma subsp. ...	[more]
KAG7031239.1	9.34e-97	80.61	Homeobox-leucine zipper protein ATHB-40 [Cucurbita argyrosperma subsp. argyrospe...	[more]

BLAST of Chy7G141060 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G36740.1	1.2e-42	55.21	homeobox protein 40	[more]
AT2G18550.1	4.5e-37	56.52	homeobox protein 21	[more]
AT5G66700.1	7.2e-35	48.73	homeobox 53	[more]
AT3G01470.1	5.0e-20	40.94	homeobox 1	[more]
AT1G69780.1	2.7e-18	41.86	Homeobox-leucine zipper protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 110..134
None	No IPR available	COILS	Coil	Coil	coord: 79..106
None	No IPR available	GENE3D	1.10.10.60		coord: 31..100 e-value: 5.3E-19 score: 69.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 48..64
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 128..151
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..64
None	No IPR available	PANTHER	PTHR24326	HOMEOBOX-LEUCINE ZIPPER PROTEIN	coord: 3..193
None	No IPR available	PANTHER	PTHR24326:SF577	HOMEOBOX-LEUCINE ZIPPER PROTEIN ATHB-40-LIKE	coord: 3..193
IPR000047	Helix-turn-helix motif	PRINTS	PR00031	HTHREPRESSR	coord: 67..83 score: 58.71 coord: 58..67 score: 46.12
IPR001356	Homeobox domain	SMART	SM00389	HOX_1	coord: 30..91 e-value: 1.3E-18 score: 77.9
IPR001356	Homeobox domain	PFAM	PF00046	Homeodomain	coord: 33..86 e-value: 3.8E-17 score: 61.9
IPR001356	Homeobox domain	PROSITE	PS50071	HOMEOBOX_2	coord: 27..87 score: 16.665388
IPR001356	Homeobox domain	CDD	cd00086	homeodomain	coord: 32..88 e-value: 5.86315E-16 score: 66.8832
IPR017970	Homeobox, conserved site	PROSITE	PS00027	HOMEOBOX_1	coord: 62..85
IPR009057	Homeobox-like domain superfamily	SUPERFAMILY	46689	Homeodomain-like	coord: 31..95

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy7G141060.1	Chy7G141060.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045893	positive regulation of transcription, DNA-templated
biological_process	GO:0006357	regulation of transcription by RNA polymerase II
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0000981	DNA-binding transcription factor activity, RNA polymerase II-specific
molecular_function	GO:0043565	sequence-specific DNA binding
molecular_function	GO:0003677	DNA binding