Chy7G138430 (gene) Cucumber (hystrix) v1

Overview
NameChy7G138430
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptiongibberellin 2-beta-dioxygenase 1
LocationchrH07: 14931474 .. 14932951 (+)
RNA-Seq ExpressionChy7G138430
SyntenyChy7G138430
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCTGCTGCTTCTTCCTCTCCAGCATTTCTCTCGGAGGTTCCATTAATAGACCTTTCTTCACCAGATGCTAAACAACTCATTGTTAAAGCCTGTGAAGAACTTGGTTTTTTCAAGGTCGTCAACCATGGCGTTCCTATGGAATTTATCTCCACTCTCGAATCAGAATCCACTAATTTCTTCTCCCTTCCCCTCTCTGAAAAACAAAAAGCTGCTCCTCCTTCCCCTTTTGGCTATGGCAACAAACAAATTGGCCGCAATGGCGACGTGGGTTGGGTCGAATACATTCTCTTAAACACTCACCTAGAATCCAACTCCGATGGGTTTCTCTCCATTTTTGGCCAAGACCCACAAAAACTCCGGTAACACAAAAACACCCAAAGCTCCATGCTCTGTTTTCTCTTACGCTCTGTTTTTTTTTCTTCTGATTTTTGTTCTCTGTTTTCCTTTTTTTTTTTTGGGTATTGTAGCTCTGCTGTGAACAATTACATATCAGCTGTGAGGAATATGGCGTGTGAAATCCTTGAGTTAATGGCGGAAGGATTGAAGATTCAGCAGAGGAATGTGTTCAGTAAACTGGTGATGGATGAAGAGAGTGACTCTGTTTTTAGGGTGAACCATTATCCGCCATGTCCACAGATTCAAGCCTTGAAAGGGATGAACATGATTGGATTTGGAGAACATACAGACCCACAGATTATATCGGTCTTAAGATCAAATAATACTTCTGGACTTCAAATTTCTCTTGCTGATGGGAATTGGATTTCTGTTCCTCCTGATCAAAACTCTTTTTTCATCAATGTTGGTGACTCTTTACAGGTACAACAATAAAACACATACATCACAGTTTCCACTAACCATTCCAAATCTTAAAAGTAAAATAAAAATTCTGTAGTTTTTTTTTTTCTCTCTATCTTTCTCTCTTGATTTGTAAATCTTTGTACTATATATATATATATATATATTCTTTAATCTCTATAGACAAGACATGATCTTAATAATCATAATTATGTCTGGTCAGCTGGCTTTGTGTTTAGGTAAAATCTCCCCAAGCCATTCATGTTGCTTCTTTTTGTTCCTTTGATAACTCAAAAAGAAACAAAATTTGAGGTTTCTTACCCTCTTTTCAAAAGCTGGTTTTTTTCCCTTAAAAAAAAAATATCATTAATGGTTCATTAAAAAAAAAAAAAAAAGAATTTGTGATTATCATGTTTGATATGTAGGTGATGACTAATGGAAGATTCAAAAGTGTGAAGCATAGGGTATTGACAAACAGCTTGAAGTCAAGAATTTCAATGATATATTTTGGTGGGCCACCATTAAGTGAAAAAATAGCTCCTTTGCCTTCACTTATGAAAGGAGAAGAAAGAAGTTTGTACAAAGAGTTTACTTGGTTTGAGTACAAAAGATCAGCTTACAACTCCAGGTTGGCAGATAACAGGCTTGTTCACTTTGAAAGAATTGCAGCCTCATAA

mRNA sequence

ATGGCTTCTGCTGCTTCTTCCTCTCCAGCATTTCTCTCGGAGGTTCCATTAATAGACCTTTCTTCACCAGATGCTAAACAACTCATTGTTAAAGCCTGTGAAGAACTTGGTTTTTTCAAGGTCGTCAACCATGGCGTTCCTATGGAATTTATCTCCACTCTCGAATCAGAATCCACTAATTTCTTCTCCCTTCCCCTCTCTGAAAAACAAAAAGCTGCTCCTCCTTCCCCTTTTGGCTATGGCAACAAACAAATTGGCCGCAATGGCGACGTGGGTTGGGTCGAATACATTCTCTTAAACACTCACCTAGAATCCAACTCCGATGGGTTTCTCTCCATTTTTGGCCAAGACCCACAAAAACTCCGCTCTGCTGTGAACAATTACATATCAGCTGTGAGGAATATGGCGTGTGAAATCCTTGAGTTAATGGCGGAAGGATTGAAGATTCAGCAGAGGAATGTGTTCAGTAAACTGGTGATGGATGAAGAGAGTGACTCTGTTTTTAGGGTGAACCATTATCCGCCATGTCCACAGATTCAAGCCTTGAAAGGGATGAACATGATTGGATTTGGAGAACATACAGACCCACAGATTATATCGGTCTTAAGATCAAATAATACTTCTGGACTTCAAATTTCTCTTGCTGATGGGAATTGGATTTCTGTTCCTCCTGATCAAAACTCTTTTTTCATCAATGTTGGTGACTCTTTACAGGTGATGACTAATGGAAGATTCAAAAGTGTGAAGCATAGGGTATTGACAAACAGCTTGAAGTCAAGAATTTCAATGATATATTTTGGTGGGCCACCATTAAGTGAAAAAATAGCTCCTTTGCCTTCACTTATGAAAGGAGAAGAAAGAAGTTTGTACAAAGAGTTTACTTGGTTTGAGTACAAAAGATCAGCTTACAACTCCAGGTTGGCAGATAACAGGCTTGTTCACTTTGAAAGAATTGCAGCCTCATAA

Coding sequence (CDS)

ATGGCTTCTGCTGCTTCTTCCTCTCCAGCATTTCTCTCGGAGGTTCCATTAATAGACCTTTCTTCACCAGATGCTAAACAACTCATTGTTAAAGCCTGTGAAGAACTTGGTTTTTTCAAGGTCGTCAACCATGGCGTTCCTATGGAATTTATCTCCACTCTCGAATCAGAATCCACTAATTTCTTCTCCCTTCCCCTCTCTGAAAAACAAAAAGCTGCTCCTCCTTCCCCTTTTGGCTATGGCAACAAACAAATTGGCCGCAATGGCGACGTGGGTTGGGTCGAATACATTCTCTTAAACACTCACCTAGAATCCAACTCCGATGGGTTTCTCTCCATTTTTGGCCAAGACCCACAAAAACTCCGCTCTGCTGTGAACAATTACATATCAGCTGTGAGGAATATGGCGTGTGAAATCCTTGAGTTAATGGCGGAAGGATTGAAGATTCAGCAGAGGAATGTGTTCAGTAAACTGGTGATGGATGAAGAGAGTGACTCTGTTTTTAGGGTGAACCATTATCCGCCATGTCCACAGATTCAAGCCTTGAAAGGGATGAACATGATTGGATTTGGAGAACATACAGACCCACAGATTATATCGGTCTTAAGATCAAATAATACTTCTGGACTTCAAATTTCTCTTGCTGATGGGAATTGGATTTCTGTTCCTCCTGATCAAAACTCTTTTTTCATCAATGTTGGTGACTCTTTACAGGTGATGACTAATGGAAGATTCAAAAGTGTGAAGCATAGGGTATTGACAAACAGCTTGAAGTCAAGAATTTCAATGATATATTTTGGTGGGCCACCATTAAGTGAAAAAATAGCTCCTTTGCCTTCACTTATGAAAGGAGAAGAAAGAAGTTTGTACAAAGAGTTTACTTGGTTTGAGTACAAAAGATCAGCTTACAACTCCAGGTTGGCAGATAACAGGCTTGTTCACTTTGAAAGAATTGCAGCCTCATAA

Protein sequence

MASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQIQALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS*
Homology
BLAST of Chy7G138430 vs. ExPASy Swiss-Prot
Match: Q9SQ80 (Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 1.8e-128
Identity = 222/311 (71.38%), Postives = 262/311 (84.24%), Query Frame = 0

Query: 11  FLSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQ 70
           F S +PL+DLS PDAK LIVKACE+ GFFKV+NHG+P++ IS LESE+  FFSLP +EK+
Sbjct: 23  FSSSIPLVDLSKPDAKTLIVKACEDFGFFKVINHGIPLDAISQLESEAFKFFSLPQTEKE 82

Query: 71  KAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQKLRSAVNNYIS 130
           KA P +PFGYGNK+IG NGD+GW+EY+LL T    N D   S++G+D  K R  + +Y  
Sbjct: 83  KAGPANPFGYGNKRIGLNGDIGWIEYLLLTT----NQDHNFSLYGEDIDKFRGLLKDYKC 142

Query: 131 AVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQIQALKGMNMIGF 190
           A+RNMACEIL+LMAEGLKIQ +NVFSKLVMD++SD +FRVNHYP CP++ A+ G N+IGF
Sbjct: 143 AMRNMACEILDLMAEGLKIQPKNVFSKLVMDKQSDCLFRVNHYPACPEL-AINGENLIGF 202

Query: 191 GEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKH 250
           GEHTDPQIIS+LRSNNTSG QISL DG+WISVPPD +SFFINVGDSLQVMTNGRFKSV+H
Sbjct: 203 GEHTDPQIISILRSNNTSGFQISLRDGSWISVPPDHSSFFINVGDSLQVMTNGRFKSVRH 262

Query: 251 RVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKRSAYNSRLADN 310
           RVL N +  R+SMIYF GPPLSEKIAPLPSLMKG+E SLYKEFTWFEYK S Y SRLADN
Sbjct: 263 RVLANGIDPRLSMIYFCGPPLSEKIAPLPSLMKGKE-SLYKEFTWFEYKSSTYGSRLADN 322

Query: 311 RLVHFERIAAS 322
           RL ++ERIAA+
Sbjct: 323 RLGNYERIAAT 327

BLAST of Chy7G138430 vs. ExPASy Swiss-Prot
Match: Q9XG83 (Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 2.6e-119
Identity = 207/314 (65.92%), Postives = 252/314 (80.25%), Query Frame = 0

Query: 7   SSPAFLSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTNFFSLPL 66
           S+P F + +P++DL+ PDAK LIV AC + GFFK+VNHGVP+E ++ LE+E+  FF    
Sbjct: 20  STPLF-TGIPVVDLTHPDAKNLIVNACRDFGFFKLVNHGVPLELMANLENEALRFFKKSQ 79

Query: 67  SEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQKLRSAVN 126
           SEK +A PP PFGYG+K+IG NGDVGWVEY+LLNT+ +  S   L IF ++P   R+ V 
Sbjct: 80  SEKDRAGPPDPFGYGSKRIGPNGDVGWVEYLLLNTNPDVISPKSLCIFRENPHHFRAVVE 139

Query: 127 NYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQIQALKGMN 186
           NYI+AV+NM   +LELMAEGL I+QRN  S+L+ DE+SDS FR+NHYPPCP++QAL   N
Sbjct: 140 NYITAVKNMCYAVLELMAEGLGIRQRNTLSRLLKDEKSDSCFRLNHYPPCPEVQAL-NRN 199

Query: 187 MIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVMTNGRFK 246
           ++GFGEHTDPQIISVLRSN+TSGLQI L DG W+SVPPDQ SFFINVGD+LQVMTNGRFK
Sbjct: 200 LVGFGEHTDPQIISVLRSNSTSGLQICLTDGTWVSVPPDQTSFFINVGDALQVMTNGRFK 259

Query: 247 SVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKRSAYNSR 306
           SVKHRVL ++ KSR+SMIYFGGP LSE IAPLPS+M   E  LYKEFTW EYK++AY SR
Sbjct: 260 SVKHRVLADTTKSRLSMIYFGGPALSENIAPLPSVMLKGEECLYKEFTWCEYKKAAYTSR 319

Query: 307 LADNRLVHFERIAA 321
           LADNRL  F++ AA
Sbjct: 320 LADNRLAPFQKSAA 331

BLAST of Chy7G138430 vs. ExPASy Swiss-Prot
Match: Q8LEA2 (Gibberellin 2-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA2OX1 PE=2 SV=2)

HSP 1 Score: 387.5 bits (994), Expect = 1.5e-106
Identity = 187/307 (60.91%), Postives = 231/307 (75.24%), Query Frame = 0

Query: 13  SEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKA 72
           S +P+ID+S P++K  +VKACE+ GFFKV+NHGV  E +S LE E+ +FFSLP SEK + 
Sbjct: 16  SLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKSEKTQV 75

Query: 73  APPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAV 132
           A   PFGYGN +IGRNGDVGWVEY+L+N + +S S        + P   R+A+  Y ++V
Sbjct: 76  A-GYPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEEYTTSV 135

Query: 133 RNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCP--QIQALKGMNMIGF 192
           R M  ++LE + +GL I+ RN  SKLV D+ +DS+ R+NHYPPCP    +   G N+IGF
Sbjct: 136 RKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGKNVIGF 195

Query: 193 GEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKH 252
           GEHTDPQIISVLRSNNTSGLQI+L DG+WISVPPD  SFF NVGDSLQVMTNGRFKSV+H
Sbjct: 196 GEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRFKSVRH 255

Query: 253 RVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKRSAYNSRLADN 312
           RVL N  KSR+SMIYF GP L+++IAPL  L+  E+  LY+EFTW EYK S YNSRL+DN
Sbjct: 256 RVLANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTYNSRLSDN 315

Query: 313 RLVHFER 318
           RL  FER
Sbjct: 316 RLQQFER 321

BLAST of Chy7G138430 vs. ExPASy Swiss-Prot
Match: Q9XFR9 (Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 2.9e-102
Identity = 177/303 (58.42%), Postives = 232/303 (76.57%), Query Frame = 0

Query: 15  VPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAP 74
           +P+++L+ P+AK  IVKACEE GFFKVVNHGV  E ++ LE E+  FF LP S K +A P
Sbjct: 31  IPVVNLADPEAKTRIVKACEEFGFFKVVNHGVRPELMTRLEQEAIGFFGLPQSLKNRAGP 90

Query: 75  PSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRN 134
           P P+GYGNK+IG NGDVGW+EY+LLN + + +S    ++F Q PQ  R +V  Y+  ++ 
Sbjct: 91  PEPYGYGNKRIGPNGDVGWIEYLLLNANPQLSSPKTSAVFRQTPQIFRESVEEYMKEIKE 150

Query: 135 MACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQIQALKGMNMIGFGEHT 194
           ++ ++LE++AE L I+ R+  SK++ DE+SDS  R+NHYP     +  + M  +GFGEHT
Sbjct: 151 VSYKVLEMVAEELGIEPRDTLSKMLRDEKSDSCLRLNHYPAAE--EEAEKMVKVGFGEHT 210

Query: 195 DPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLT 254
           DPQIISVLRSNNT+GLQI + DG+W++VPPD +SFFINVGD+LQVMTNGRFKSVKHRVL 
Sbjct: 211 DPQIISVLRSNNTAGLQICVKDGSWVAVPPDHSSFFINVGDALQVMTNGRFKSVKHRVLA 270

Query: 255 NSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKRSAYNSRLADNRLVH 314
           ++ +SRISMIYFGGPPLS+KIAPLP L+  ++  LYKEFTW +YK SAY S+L D RL  
Sbjct: 271 DTRRSRISMIYFGGPPLSQKIAPLPCLVPEQDDWLYKEFTWSQYKSSAYKSKLGDYRLGL 330

Query: 315 FER 318
           FE+
Sbjct: 331 FEK 331

BLAST of Chy7G138430 vs. ExPASy Swiss-Prot
Match: Q8S0S6 (Gibberellin 2-beta-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX3 PE=1 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 4.6e-100
Identity = 180/305 (59.02%), Postives = 225/305 (73.77%), Query Frame = 0

Query: 13  SEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKA 72
           S VP++DL SP A + +V ACE  GFFKVVNHGV  + +   ESE+  FFS    +K ++
Sbjct: 26  SGVPVVDLGSPGAARAVVDACERYGFFKVVNHGVATDTMDKAESEAVRFFSQTQPDKDRS 85

Query: 73  APPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAV 132
            P  PFGYG+K+IG NGD+GW+EY+LL     S +D   +         R+A+N YIS V
Sbjct: 86  GPAYPFGYGSKRIGFNGDMGWLEYLLLALDDASLAD---ACTVPSCAVFRAALNEYISGV 145

Query: 133 RNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQIQALKGMNMIGFGE 192
           R +A  ++E M+EGL I Q +  S LV  E SD VFRVNHYPPC  +Q L G ++ GFGE
Sbjct: 146 RKVAVRVMEAMSEGLGIAQADALSALVTAEGSDQVFRVNHYPPCRALQGL-GCSVTGFGE 205

Query: 193 HTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRV 252
           HTDPQ++SVLRSN TSGLQI+L DG W+SVP D++SFF+NVGDSLQV+TNGRFKSVKHRV
Sbjct: 206 HTDPQLVSVLRSNGTSGLQIALRDGQWVSVPSDRDSFFVNVGDSLQVLTNGRFKSVKHRV 265

Query: 253 LTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKRSAYNSRLADNRL 312
           + NSLKSR+S IYFGGPPL+++IAPLP L+   E+SLYKEFTW EYK++AY SRL DNRL
Sbjct: 266 VANSLKSRVSFIYFGGPPLAQRIAPLPQLLGEGEQSLYKEFTWDEYKKAAYKSRLGDNRL 325

Query: 313 VHFER 318
             FE+
Sbjct: 326 AQFEK 326

BLAST of Chy7G138430 vs. ExPASy TrEMBL
Match: A0A0A0KXI6 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G075200 PE=3 SV=1)

HSP 1 Score: 642.1 bits (1655), Expect = 1.2e-180
Identity = 321/321 (100.00%), Postives = 321/321 (100.00%), Query Frame = 0

Query: 1   MASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN 60
           MASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN
Sbjct: 22  MASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN 81

Query: 61  FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQK 120
           FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQK
Sbjct: 82  FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQK 141

Query: 121 LRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQIQ 180
           LRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQIQ
Sbjct: 142 LRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQIQ 201

Query: 181 ALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM 240
           ALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM
Sbjct: 202 ALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM 261

Query: 241 TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR 300
           TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR
Sbjct: 262 TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR 321

Query: 301 SAYNSRLADNRLVHFERIAAS 322
           SAYNSRLADNRLVHFERIAAS
Sbjct: 322 SAYNSRLADNRLVHFERIAAS 342

BLAST of Chy7G138430 vs. ExPASy TrEMBL
Match: S0BDZ9 (GA 2-oxidase OS=Cucumis sativus OX=3659 GN=GA2ox1 PE=2 SV=1)

HSP 1 Score: 642.1 bits (1655), Expect = 1.2e-180
Identity = 321/321 (100.00%), Postives = 321/321 (100.00%), Query Frame = 0

Query: 1   MASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN 60
           MASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN
Sbjct: 1   MASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN 60

Query: 61  FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQK 120
           FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQK
Sbjct: 61  FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQK 120

Query: 121 LRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQIQ 180
           LRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQIQ
Sbjct: 121 LRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQIQ 180

Query: 181 ALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM 240
           ALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM
Sbjct: 181 ALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM 240

Query: 241 TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR 300
           TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR
Sbjct: 241 TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR 300

Query: 301 SAYNSRLADNRLVHFERIAAS 322
           SAYNSRLADNRLVHFERIAAS
Sbjct: 301 SAYNSRLADNRLVHFERIAAS 321

BLAST of Chy7G138430 vs. ExPASy TrEMBL
Match: A0A5A7V705 (Gibberellin 2-beta-dioxygenase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G001240 PE=3 SV=1)

HSP 1 Score: 636.0 bits (1639), Expect = 8.8e-179
Identity = 318/321 (99.07%), Postives = 318/321 (99.07%), Query Frame = 0

Query: 1   MASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN 60
           MASAASSS AF SEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN
Sbjct: 1   MASAASSSAAFFSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN 60

Query: 61  FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQK 120
           FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQK
Sbjct: 61  FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQK 120

Query: 121 LRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQIQ 180
           LRSAVNNYISAVRNMACEILELMAEGLKIQQRN FSKLVMDEESDSVFRVNHYPPCPQIQ
Sbjct: 121 LRSAVNNYISAVRNMACEILELMAEGLKIQQRNAFSKLVMDEESDSVFRVNHYPPCPQIQ 180

Query: 181 ALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM 240
           ALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM
Sbjct: 181 ALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM 240

Query: 241 TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR 300
           TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR
Sbjct: 241 TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR 300

Query: 301 SAYNSRLADNRLVHFERIAAS 322
           SAYNSRLADNRLVHFERIAAS
Sbjct: 301 SAYNSRLADNRLVHFERIAAS 321

BLAST of Chy7G138430 vs. ExPASy TrEMBL
Match: A0A1S3BRQ7 (gibberellin 2-beta-dioxygenase 1 OS=Cucumis melo OX=3656 GN=LOC103492969 PE=3 SV=1)

HSP 1 Score: 636.0 bits (1639), Expect = 8.8e-179
Identity = 318/321 (99.07%), Postives = 318/321 (99.07%), Query Frame = 0

Query: 1   MASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN 60
           MASAASSS AF SEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN
Sbjct: 22  MASAASSSAAFFSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN 81

Query: 61  FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQK 120
           FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQK
Sbjct: 82  FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQK 141

Query: 121 LRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQIQ 180
           LRSAVNNYISAVRNMACEILELMAEGLKIQQRN FSKLVMDEESDSVFRVNHYPPCPQIQ
Sbjct: 142 LRSAVNNYISAVRNMACEILELMAEGLKIQQRNAFSKLVMDEESDSVFRVNHYPPCPQIQ 201

Query: 181 ALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM 240
           ALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM
Sbjct: 202 ALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM 261

Query: 241 TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR 300
           TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR
Sbjct: 262 TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR 321

Query: 301 SAYNSRLADNRLVHFERIAAS 322
           SAYNSRLADNRLVHFERIAAS
Sbjct: 322 SAYNSRLADNRLVHFERIAAS 342

BLAST of Chy7G138430 vs. ExPASy TrEMBL
Match: A0A6J1FNB4 (gibberellin 2-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111447347 PE=3 SV=1)

HSP 1 Score: 602.1 bits (1551), Expect = 1.4e-168
Identity = 299/321 (93.15%), Postives = 309/321 (96.26%), Query Frame = 0

Query: 1   MASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN 60
           MA+A SSS AF S VPLIDLS+PDAK LIVKACEELGFFKVVNHGVPMEFISTLESEST 
Sbjct: 21  MAAAGSSSAAFFSGVPLIDLSAPDAKHLIVKACEELGFFKVVNHGVPMEFISTLESESTK 80

Query: 61  FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQK 120
           FFSLPLSEKQ+A PPSPFGYGNKQIGRNGDVGWVEY+LLNTHLESNSDGFLSIFGQDPQK
Sbjct: 81  FFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQK 140

Query: 121 LRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQIQ 180
           LRSAVN+YISAVRNMACEILELMAEGLKIQQRN FSKL+MDEESDSVFRVNHYPPCP++Q
Sbjct: 141 LRSAVNDYISAVRNMACEILELMAEGLKIQQRNAFSKLLMDEESDSVFRVNHYPPCPELQ 200

Query: 181 ALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM 240
            LKG NMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQ+SFFINVGDSLQVM
Sbjct: 201 TLKGTNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQSSFFINVGDSLQVM 260

Query: 241 TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR 300
           TNGRFKSVKHRVLTN LKSR+SMIYFGGPPLSEKIAPLPSLMKGEE SLYKEFTWFEYKR
Sbjct: 261 TNGRFKSVKHRVLTNRLKSRVSMIYFGGPPLSEKIAPLPSLMKGEE-SLYKEFTWFEYKR 320

Query: 301 SAYNSRLADNRLVHFERIAAS 322
           SAYNSRLADNRLVHFERIAAS
Sbjct: 321 SAYNSRLADNRLVHFERIAAS 340

BLAST of Chy7G138430 vs. NCBI nr
Match: NP_001292640.1 (gibberellin 2-beta-dioxygenase 1 [Cucumis sativus] >CCD28485.1 GA 2-oxidase [Cucumis sativus])

HSP 1 Score: 639 bits (1649), Expect = 3.26e-231
Identity = 321/321 (100.00%), Postives = 321/321 (100.00%), Query Frame = 0

Query: 1   MASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN 60
           MASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN
Sbjct: 1   MASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN 60

Query: 61  FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQK 120
           FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQK
Sbjct: 61  FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQK 120

Query: 121 LRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQIQ 180
           LRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQIQ
Sbjct: 121 LRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQIQ 180

Query: 181 ALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM 240
           ALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM
Sbjct: 181 ALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM 240

Query: 241 TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR 300
           TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR
Sbjct: 241 TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR 300

Query: 301 SAYNSRLADNRLVHFERIAAS 321
           SAYNSRLADNRLVHFERIAAS
Sbjct: 301 SAYNSRLADNRLVHFERIAAS 321

BLAST of Chy7G138430 vs. NCBI nr
Match: KGN53539.1 (hypothetical protein Csa_014892 [Cucumis sativus])

HSP 1 Score: 639 bits (1649), Expect = 7.14e-231
Identity = 321/321 (100.00%), Postives = 321/321 (100.00%), Query Frame = 0

Query: 1   MASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN 60
           MASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN
Sbjct: 22  MASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN 81

Query: 61  FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQK 120
           FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQK
Sbjct: 82  FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQK 141

Query: 121 LRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQIQ 180
           LRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQIQ
Sbjct: 142 LRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQIQ 201

Query: 181 ALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM 240
           ALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM
Sbjct: 202 ALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM 261

Query: 241 TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR 300
           TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR
Sbjct: 262 TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR 321

Query: 301 SAYNSRLADNRLVHFERIAAS 321
           SAYNSRLADNRLVHFERIAAS
Sbjct: 322 SAYNSRLADNRLVHFERIAAS 342

BLAST of Chy7G138430 vs. NCBI nr
Match: KAA0063004.1 (gibberellin 2-beta-dioxygenase 1 [Cucumis melo var. makuwa] >TYK16342.1 gibberellin 2-beta-dioxygenase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 633 bits (1633), Expect = 8.96e-229
Identity = 318/321 (99.07%), Postives = 318/321 (99.07%), Query Frame = 0

Query: 1   MASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN 60
           MASAASSS AF SEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN
Sbjct: 1   MASAASSSAAFFSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN 60

Query: 61  FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQK 120
           FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQK
Sbjct: 61  FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQK 120

Query: 121 LRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQIQ 180
           LRSAVNNYISAVRNMACEILELMAEGLKIQQRN FSKLVMDEESDSVFRVNHYPPCPQIQ
Sbjct: 121 LRSAVNNYISAVRNMACEILELMAEGLKIQQRNAFSKLVMDEESDSVFRVNHYPPCPQIQ 180

Query: 181 ALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM 240
           ALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM
Sbjct: 181 ALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM 240

Query: 241 TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR 300
           TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR
Sbjct: 241 TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR 300

Query: 301 SAYNSRLADNRLVHFERIAAS 321
           SAYNSRLADNRLVHFERIAAS
Sbjct: 301 SAYNSRLADNRLVHFERIAAS 321

BLAST of Chy7G138430 vs. NCBI nr
Match: XP_008451787.1 (PREDICTED: gibberellin 2-beta-dioxygenase 1 [Cucumis melo])

HSP 1 Score: 633 bits (1633), Expect = 1.96e-228
Identity = 318/321 (99.07%), Postives = 318/321 (99.07%), Query Frame = 0

Query: 1   MASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN 60
           MASAASSS AF SEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN
Sbjct: 22  MASAASSSAAFFSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN 81

Query: 61  FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQK 120
           FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQK
Sbjct: 82  FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQK 141

Query: 121 LRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQIQ 180
           LRSAVNNYISAVRNMACEILELMAEGLKIQQRN FSKLVMDEESDSVFRVNHYPPCPQIQ
Sbjct: 142 LRSAVNNYISAVRNMACEILELMAEGLKIQQRNAFSKLVMDEESDSVFRVNHYPPCPQIQ 201

Query: 181 ALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM 240
           ALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM
Sbjct: 202 ALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM 261

Query: 241 TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR 300
           TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR
Sbjct: 262 TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR 321

Query: 301 SAYNSRLADNRLVHFERIAAS 321
           SAYNSRLADNRLVHFERIAAS
Sbjct: 322 SAYNSRLADNRLVHFERIAAS 342

BLAST of Chy7G138430 vs. NCBI nr
Match: XP_038879745.1 (gibberellin 2-beta-dioxygenase 1-like [Benincasa hispida])

HSP 1 Score: 624 bits (1610), Expect = 7.02e-225
Identity = 312/321 (97.20%), Postives = 317/321 (98.75%), Query Frame = 0

Query: 1   MASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN 60
           MA+AASSS AF +EVPLIDLS+PDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN
Sbjct: 25  MAAAASSSAAFFTEVPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN 84

Query: 61  FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQK 120
           FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEY+LLNTHLESNSDGFLSIFGQDPQK
Sbjct: 85  FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQK 144

Query: 121 LRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQIQ 180
           LRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCP+ Q
Sbjct: 145 LRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPEPQ 204

Query: 181 ALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM 240
           ALKG NMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM
Sbjct: 205 ALKGRNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM 264

Query: 241 TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR 300
           TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR
Sbjct: 265 TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR 324

Query: 301 SAYNSRLADNRLVHFERIAAS 321
           SAYNSRLADNRLVHFERIAAS
Sbjct: 325 SAYNSRLADNRLVHFERIAAS 345

BLAST of Chy7G138430 vs. TAIR 10
Match: AT1G78440.1 (Arabidopsis thaliana gibberellin 2-oxidase 1 )

HSP 1 Score: 387.5 bits (994), Expect = 1.0e-107
Identity = 187/307 (60.91%), Postives = 231/307 (75.24%), Query Frame = 0

Query: 13  SEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKA 72
           S +P+ID+S P++K  +VKACE+ GFFKV+NHGV  E +S LE E+ +FFSLP SEK + 
Sbjct: 16  SLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKSEKTQV 75

Query: 73  APPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAV 132
           A   PFGYGN +IGRNGDVGWVEY+L+N + +S S        + P   R+A+  Y ++V
Sbjct: 76  A-GYPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEEYTTSV 135

Query: 133 RNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCP--QIQALKGMNMIGF 192
           R M  ++LE + +GL I+ RN  SKLV D+ +DS+ R+NHYPPCP    +   G N+IGF
Sbjct: 136 RKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGKNVIGF 195

Query: 193 GEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKH 252
           GEHTDPQIISVLRSNNTSGLQI+L DG+WISVPPD  SFF NVGDSLQVMTNGRFKSV+H
Sbjct: 196 GEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRFKSVRH 255

Query: 253 RVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKRSAYNSRLADN 312
           RVL N  KSR+SMIYF GP L+++IAPL  L+  E+  LY+EFTW EYK S YNSRL+DN
Sbjct: 256 RVLANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTYNSRLSDN 315

Query: 313 RLVHFER 318
           RL  FER
Sbjct: 316 RLQQFER 321

BLAST of Chy7G138430 vs. TAIR 10
Match: AT1G30040.1 (gibberellin 2-oxidase )

HSP 1 Score: 373.2 bits (957), Expect = 2.0e-103
Identity = 177/303 (58.42%), Postives = 232/303 (76.57%), Query Frame = 0

Query: 15  VPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAP 74
           +P+++L+ P+AK  IVKACEE GFFKVVNHGV  E ++ LE E+  FF LP S K +A P
Sbjct: 31  IPVVNLADPEAKTRIVKACEEFGFFKVVNHGVRPELMTRLEQEAIGFFGLPQSLKNRAGP 90

Query: 75  PSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRN 134
           P P+GYGNK+IG NGDVGW+EY+LLN + + +S    ++F Q PQ  R +V  Y+  ++ 
Sbjct: 91  PEPYGYGNKRIGPNGDVGWIEYLLLNANPQLSSPKTSAVFRQTPQIFRESVEEYMKEIKE 150

Query: 135 MACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQIQALKGMNMIGFGEHT 194
           ++ ++LE++AE L I+ R+  SK++ DE+SDS  R+NHYP     +  + M  +GFGEHT
Sbjct: 151 VSYKVLEMVAEELGIEPRDTLSKMLRDEKSDSCLRLNHYPAAE--EEAEKMVKVGFGEHT 210

Query: 195 DPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLT 254
           DPQIISVLRSNNT+GLQI + DG+W++VPPD +SFFINVGD+LQVMTNGRFKSVKHRVL 
Sbjct: 211 DPQIISVLRSNNTAGLQICVKDGSWVAVPPDHSSFFINVGDALQVMTNGRFKSVKHRVLA 270

Query: 255 NSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKRSAYNSRLADNRLVH 314
           ++ +SRISMIYFGGPPLS+KIAPLP L+  ++  LYKEFTW +YK SAY S+L D RL  
Sbjct: 271 DTRRSRISMIYFGGPPLSQKIAPLPCLVPEQDDWLYKEFTWSQYKSSAYKSKLGDYRLGL 330

Query: 315 FER 318
           FE+
Sbjct: 331 FEK 331

BLAST of Chy7G138430 vs. TAIR 10
Match: AT2G34555.1 (gibberellin 2-oxidase 3 )

HSP 1 Score: 346.3 bits (887), Expect = 2.7e-95
Identity = 172/303 (56.77%), Postives = 217/303 (71.62%), Query Frame = 0

Query: 15  VPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAP 74
           +P+IDL+  DAK  IVKACEE GFFKV+NHGV  + ++ LE E+ NFF+L  S K KA P
Sbjct: 27  IPVIDLTDSDAKTQIVKACEEFGFFKVINHGVRPDLLTQLEQEAINFFALHHSLKDKAGP 86

Query: 75  PSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRN 134
           P PFGYG K+IG NGD+GW+EYILLN +L   S    +IF   P   R AV  YI  ++ 
Sbjct: 87  PDPFGYGTKRIGPNGDLGWLEYILLNANLCLESHKTTAIFRHTPAIFREAVEEYIKEMKR 146

Query: 135 MACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQIQALKGMNMIGFGEHT 194
           M+ + LE++ E LKI+ +   S+LV  +ESDS  R+NHY   P+ +       IGFGEHT
Sbjct: 147 MSSKFLEMVEEELKIEPKEKLSRLVKVKESDSCLRMNHY---PEKEETPVKEEIGFGEHT 206

Query: 195 DPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLT 254
           DPQ+IS+LRSN+T GLQI + DG W+ V PD +SFF+ VGD+LQVMTNGRFKSVKHRV+T
Sbjct: 207 DPQLISLLRSNDTEGLQICVKDGTWVDVTPDHSSFFVLVGDTLQVMTNGRFKSVKHRVVT 266

Query: 255 NSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKRSAYNSRLADNRLVH 314
           N+ +SRISMIYF GPPLSEKIAPL  L+  ++  LY EFTW +YK SAY ++L D RL  
Sbjct: 267 NTKRSRISMIYFAGPPLSEKIAPLSCLVPKQDDCLYNEFTWSQYKLSAYKTKLGDYRLGL 326

Query: 315 FER 318
           FE+
Sbjct: 327 FEK 326

BLAST of Chy7G138430 vs. TAIR 10
Match: AT1G02400.1 (gibberellin 2-oxidase 6 )

HSP 1 Score: 300.1 bits (767), Expect = 2.2e-81
Identity = 157/316 (49.68%), Postives = 205/316 (64.87%), Query Frame = 0

Query: 7   SSPAFLSEVPLIDLSSPDAKQL---IVKACEELGFFKVVNHGVPMEFISTLESESTNFFS 66
           SSP +    P+ID S  D  +L   IVKACE  GFFKV+NHGV  E I   E E   FF+
Sbjct: 18  SSPEY--NFPVIDFSLNDRSKLSEKIVKACEVNGFFKVINHGVKPEIIKRFEHEGEEFFN 77

Query: 67  LPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQKLRS 126
            P S+K +A P SPFGYG K IG NGD+G +EY+LL+ +  + +D   +I   DP K  S
Sbjct: 78  KPESDKLRAGPASPFGYGCKNIGFNGDLGELEYLLLHANPTAVADKSETISHDDPFKFSS 137

Query: 127 AVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQIQALK 186
           A N+YI  VR++ACEI++L  E L  Q+ +  S+L+ D  SDS+ R+NHYPP P   AL 
Sbjct: 138 ATNDYIRTVRDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSILRLNHYPPAP--YALS 197

Query: 187 GMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVMTNG 246
           G+  IGFGEH+DPQI++VLRSN+  GL+I   DG WI +P D   FF+ VGD LQ +TNG
Sbjct: 198 GVGQIGFGEHSDPQILTVLRSNDVDGLEICSRDGLWIPIPSDPTCFFVLVGDCLQALTNG 257

Query: 247 RFKSVKHRVLTNSLKS-RISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKRSA 306
           RF SV+HRVL N+ K  R+S +YF  PPL  KI+PLP ++  E    Y  FTW +YK++ 
Sbjct: 258 RFTSVRHRVLANTAKKPRMSAMYFAAPPLEAKISPLPKMVSPENPRRYNSFTWGDYKKAT 317

Query: 307 YNSRLADNRLVHFERI 319
           Y+ RL   RL  F+ +
Sbjct: 318 YSLRLDVPRLEFFKTL 329

BLAST of Chy7G138430 vs. TAIR 10
Match: AT1G47990.1 (gibberellin 2-oxidase 4 )

HSP 1 Score: 283.5 bits (724), Expect = 2.1e-76
Identity = 160/326 (49.08%), Postives = 201/326 (61.66%), Query Frame = 0

Query: 1   MASAASSSPAFLSEVPLIDLSS--PDAKQLIVKACEELGFFKVVNHGVPMEFISTLESES 60
           M   +    A   ++P+ID+S         IVKACE LGFFKV+NHGV    IS +E ES
Sbjct: 1   MVKGSQKIVAVDQDIPIIDMSQERSQVSMQIVKACESLGFFKVINHGVDQTTISRMEQES 60

Query: 61  TNFFSLPLSEKQKAAPPS-PFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQD 120
            NFF+ P  EK+   P + PF YG + IG NGD G VEY+L +T    N   F S     
Sbjct: 61  INFFAKPAHEKKSVRPVNQPFRYGFRDIGLNGDSGEVEYLLFHT----NDPAFRS----- 120

Query: 121 PQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCP 180
                SAVN YI AV+ +A EIL+L AEGL +   + FS+L+   +SDSV RVNHYPP  
Sbjct: 121 QLSFSSAVNCYIEAVKQLAREILDLTAEGLHVPPHS-FSRLISSVDSDSVLRVNHYPPSD 180

Query: 181 QI-------QALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFF 240
           Q             +  +GFGEHTDPQI++VLRSN   GLQ+S +DG W+SV PD ++F 
Sbjct: 181 QFFGEANLSDQSVSLTRVGFGEHTDPQILTVLRSNGVGGLQVSNSDGMWVSVSPDPSAFC 240

Query: 241 INVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSL-MKGEERSL 300
           +NVGD LQVMTNGRF SV+HR LT   +SR+S  YF GPPL  KI PL ++ M   +  L
Sbjct: 241 VNVGDLLQVMTNGRFISVRHRALTYGEESRLSTAYFAGPPLQAKIGPLSAMVMTMNQPRL 300

Query: 301 YKEFTWFEYKRSAYNSRLADNRLVHF 316
           Y+ FTW EYK+ AY+ RL D+RL  F
Sbjct: 301 YQTFTWGEYKKRAYSLRLEDSRLDMF 316

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SQ801.8e-12871.38Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1[more]
Q9XG832.6e-11965.92Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=... [more]
Q8LEA21.5e-10660.91Gibberellin 2-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA2OX1 PE=2 ... [more]
Q9XFR92.9e-10258.42Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 ... [more]
Q8S0S64.6e-10059.02Gibberellin 2-beta-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2... [more]
Match NameE-valueIdentityDescription
A0A0A0KXI61.2e-180100.00Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G... [more]
S0BDZ91.2e-180100.00GA 2-oxidase OS=Cucumis sativus OX=3659 GN=GA2ox1 PE=2 SV=1[more]
A0A5A7V7058.8e-17999.07Gibberellin 2-beta-dioxygenase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3BRQ78.8e-17999.07gibberellin 2-beta-dioxygenase 1 OS=Cucumis melo OX=3656 GN=LOC103492969 PE=3 SV... [more]
A0A6J1FNB41.4e-16893.15gibberellin 2-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111447... [more]
Match NameE-valueIdentityDescription
NP_001292640.13.26e-231100.00gibberellin 2-beta-dioxygenase 1 [Cucumis sativus] >CCD28485.1 GA 2-oxidase [Cuc... [more]
KGN53539.17.14e-231100.00hypothetical protein Csa_014892 [Cucumis sativus][more]
KAA0063004.18.96e-22999.07gibberellin 2-beta-dioxygenase 1 [Cucumis melo var. makuwa] >TYK16342.1 gibberel... [more]
XP_008451787.11.96e-22899.07PREDICTED: gibberellin 2-beta-dioxygenase 1 [Cucumis melo][more]
XP_038879745.17.02e-22597.20gibberellin 2-beta-dioxygenase 1-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G78440.11.0e-10760.91Arabidopsis thaliana gibberellin 2-oxidase 1 [more]
AT1G30040.12.0e-10358.42gibberellin 2-oxidase [more]
AT2G34555.12.7e-9556.77gibberellin 2-oxidase 3 [more]
AT1G02400.12.2e-8149.68gibberellin 2-oxidase 6 [more]
AT1G47990.12.1e-7649.08gibberellin 2-oxidase 4 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00682IPNSYNTHASEcoord: 29..46
score: 54.55
coord: 193..214
score: 32.23
coord: 229..255
score: 33.0
NoneNo IPR availablePANTHERPTHR47990:SF150GIBBERELLIN 2-BETA-DIOXYGENASE 1coord: 12..318
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 12..318
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 12..309
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 15..106
e-value: 2.0E-16
score: 60.8
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 2..309
e-value: 7.0E-82
score: 277.4
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 165..267
e-value: 2.6E-27
score: 95.2
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 163..269
score: 12.434464

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy7G138430.1Chy7G138430.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045487 gibberellin catabolic process
biological_process GO:0009416 response to light stimulus
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0052634 C-19 gibberellin 2-beta-dioxygenase activity
molecular_function GO:0046872 metal ion binding