Chy7G135200 (gene) Cucumber (hystrix) v1

Overview


Name	Chy7G135200
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Glucan endo-1,3-beta-glucosidase
Location	chrH07: 6683113 .. 6684765 (-)
RNA-Seq Expression	Chy7G135200
Synteny	Chy7G135200

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCCTTATTCTTCCCTACAACTTCCCTTTTGTTCATCTTCATATTTTCCCATCAATTCCTTCATAGAAGTAATTAACAATCCTAAAGTTTTATATCCACAAATTTTGTTAATTTGTTCCTTTTTTTAATACGTTGGAATTTTGTGTTACAGGTGTGTTTGGCGCAGTTGGAGTGAACTATGGGTTGAATAGTGACAATCTTCCAAAACCAAATGAAGTGATAAACTTATACGAACGATGTGGGATCAATATTGTTCGGATATTCGAGCCCAACCATGAGATTCTTCATGCTTTGTGTGGCAAGGAAAATTTAGTGTTGTGGCTAGGTACAAGAAATGAAGACATTGAGGGGTTTGCTACAAATCAAGAAGTGGCTAATGCTTGGGTTAATGCTAATGTGGTTCCTTATTACAAGGATGTCAATATTGCTTATATCACTGTTGGCAATGAAGTTGTCCCTGGCGATACAGCTTCACCATTTGTTGCTAATGCCATTAAAAATATGATGCAAGCGCTTGATAATGCGGGCGTCCAAAGCGACATCAAGGTAATGAATGATTTATTGAATTAAATTATGAATTTGGTTTTGTTACATTTTTCATTTGATTGGATATTGTTTTGATTTCTTCTTTACTATTATTATGAGTTTGGAATACACTTTCATTTAAAACATAAACCATTTTAGAAAAACAAATAGAACATCAAAAGGGTAGGTATTTCATAAGAGATTAAATAAAAATGATTTTTTTGTAGTGAGGTATGTTTCAAAGTTTTTATAAAATAATAATAATAAATAATAATAATAATTTTGAGTGGTAGGCAGATTTAATTTGATTAATGAGATTTGGATTTAGATTCATTTTATCCCTTATAAAATCAGTTGCAATGTGAATAATATTTGAGTTAGGTTTTCTATATATAGTCTAATCTTTATAATTTTAAAATTTTGGATTCGTATTCTATATTTGGACTTAGGCGTGAACACCATTTTCAAAAGGAATAATGTGTAACTTTTATGATCTCTTAATTCCAAGTCCAAGTACACAAAAGATTGTAGTTTTTAGTAAATTAAACATTTACAGGTGACGACAGTGGTAGCCATGACAGTATTGGAGGTGTCATCCCCACCTTCGGCCGGAGCATTCTCAGCCATCGCCGCTAGAGCAATGAAAGACATCGGAAATGTGTTAGAATCTTCATGTGCACCAATATTGGTGAATGTGTACCCATATTTCGCTTACGCATCAAACCCACAACAAATATCACTCAGTTACGCATTGTTCACATCCACAAGCCCGGTCGTGGTAGACGGAGACTTGCAGTACTTCAATCTGTTGGATGCCATGGTGGATTCATTCTATGCAGCGCTGGAGAAGATTGGCGTTGAGGGAGTGAGAATCGGGATATCAGAGACTGGGTGGCCCACCAAAGGAAACGAGCCATTCACGAGCGTTGAAAATGCATTGACTTATAACAAAAATTTTGTGGAACATGTGACCAGTGGAGTTGGAACCCCCAGAATGCCCAATCTCCAATATGACGTCGTTTTGTTTGAAATGTTCAATGAAGATCTTAAGTCGCCTGGAGTTGAGCAGAATTTTGGCTTCTTTGATCCCAGTATGAACCCAGTTTATTCTTTTTGGAATTGTTGA

mRNA sequence

ATGCCCTTATTCTTCCCTACAACTTCCCTTTTGTTCATCTTCATATTTTCCCATCAATTCCTTCATAGAAGTGTGTTTGGCGCAGTTGGAGTGAACTATGGGTTGAATAGTGACAATCTTCCAAAACCAAATGAAGTGATAAACTTATACGAACGATGTGGGATCAATATTGTTCGGATATTCGAGCCCAACCATGAGATTCTTCATGCTTTGTGTGGCAAGGAAAATTTAGTGTTGTGGCTAGGTACAAGAAATGAAGACATTGAGGGGTTTGCTACAAATCAAGAAGTGGCTAATGCTTGGGTTAATGCTAATGTGGTTCCTTATTACAAGGATGTCAATATTGCTTATATCACTGTTGGCAATGAAGTTGTCCCTGGCGATACAGCTTCACCATTTGTTGCTAATGCCATTAAAAATATGATGCAAGCGCTTGATAATGCGGGCGTCCAAAGCGACATCAAGGTGACGACAGTGGTAGCCATGACAGTATTGGAGGTGTCATCCCCACCTTCGGCCGGAGCATTCTCAGCCATCGCCGCTAGAGCAATGAAAGACATCGGAAATGTGTTAGAATCTTCATGTGCACCAATATTGGTGAATGTGTACCCATATTTCGCTTACGCATCAAACCCACAACAAATATCACTCAGTTACGCATTGTTCACATCCACAAGCCCGGTCGTGGTAGACGGAGACTTGCAGTACTTCAATCTGTTGGATGCCATGGTGGATTCATTCTATGCAGCGCTGGAGAAGATTGGCGTTGAGGGAGTGAGAATCGGGATATCAGAGACTGGGTGGCCCACCAAAGGAAACGAGCCATTCACGAGCGTTGAAAATGCATTGACTTATAACAAAAATTTTGTGGAACATGTGACCAGTGGAGTTGGAACCCCCAGAATGCCCAATCTCCAATATGACGTCGTTTTGTTTGAAATGTTCAATGAAGATCTTAAGTCGCCTGGAGTTGAGCAGAATTTTGGCTTCTTTGATCCCAGTATGAACCCAGTTTATTCTTTTTGGAATTGTTGA

Coding sequence (CDS)

ATGCCCTTATTCTTCCCTACAACTTCCCTTTTGTTCATCTTCATATTTTCCCATCAATTCCTTCATAGAAGTGTGTTTGGCGCAGTTGGAGTGAACTATGGGTTGAATAGTGACAATCTTCCAAAACCAAATGAAGTGATAAACTTATACGAACGATGTGGGATCAATATTGTTCGGATATTCGAGCCCAACCATGAGATTCTTCATGCTTTGTGTGGCAAGGAAAATTTAGTGTTGTGGCTAGGTACAAGAAATGAAGACATTGAGGGGTTTGCTACAAATCAAGAAGTGGCTAATGCTTGGGTTAATGCTAATGTGGTTCCTTATTACAAGGATGTCAATATTGCTTATATCACTGTTGGCAATGAAGTTGTCCCTGGCGATACAGCTTCACCATTTGTTGCTAATGCCATTAAAAATATGATGCAAGCGCTTGATAATGCGGGCGTCCAAAGCGACATCAAGGTGACGACAGTGGTAGCCATGACAGTATTGGAGGTGTCATCCCCACCTTCGGCCGGAGCATTCTCAGCCATCGCCGCTAGAGCAATGAAAGACATCGGAAATGTGTTAGAATCTTCATGTGCACCAATATTGGTGAATGTGTACCCATATTTCGCTTACGCATCAAACCCACAACAAATATCACTCAGTTACGCATTGTTCACATCCACAAGCCCGGTCGTGGTAGACGGAGACTTGCAGTACTTCAATCTGTTGGATGCCATGGTGGATTCATTCTATGCAGCGCTGGAGAAGATTGGCGTTGAGGGAGTGAGAATCGGGATATCAGAGACTGGGTGGCCCACCAAAGGAAACGAGCCATTCACGAGCGTTGAAAATGCATTGACTTATAACAAAAATTTTGTGGAACATGTGACCAGTGGAGTTGGAACCCCCAGAATGCCCAATCTCCAATATGACGTCGTTTTGTTTGAAATGTTCAATGAAGATCTTAAGTCGCCTGGAGTTGAGCAGAATTTTGGCTTCTTTGATCCCAGTATGAACCCAGTTTATTCTTTTTGGAATTGTTGA

Protein sequence

MPLFFPTTSLLFIFIFSHQFLHRSVFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRIFEPNHEILHALCGKENLVLWLGTRNEDIEGFATNQEVANAWVNANVVPYYKDVNIAYITVGNEVVPGDTASPFVANAIKNMMQALDNAGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIAARAMKDIGNVLESSCAPILVNVYPYFAYASNPQQISLSYALFTSTSPVVVDGDLQYFNLLDAMVDSFYAALEKIGVEGVRIGISETGWPTKGNEPFTSVENALTYNKNFVEHVTSGVGTPRMPNLQYDVVLFEMFNEDLKSPGVEQNFGFFDPSMNPVYSFWNC*

Homology

[Close]

BLAST of Chy7G135200 vs. ExPASy Swiss-Prot
Match: O49352 (Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana OX=3702 GN=BG4 PE=2 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 3.8e-76
Identity = 153/344 (44.48%), Postives = 219/344 (63.66%), Query Frame = 0


Query: 3   LFFPTTSLLFIFIFSHQFLHRSVFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRIFE 62
           LF    S + +++ S+     +   ++G+NYGL  DNLP P+ VINLY+  GI+ +RIF+
Sbjct: 8   LFLFFLSCIVLYVNSNNSGFVTAANSIGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFD 67

Query: 63  PNHEILHALCGKENLVLWLGTRNEDIEGFATNQEVANAWVNANVVPYYKDVNIAYITVGN 122
           PN E+L+AL G  ++ + +G +++D+   A ++E    W  AN+  Y  DVNI +ITVGN
Sbjct: 68  PNTEVLNALRGHRDIEVTVGVKDQDLAALAASEEAVKGWFAANIESYLADVNITFITVGN 127

Query: 123 EVVPGDTASPFVANAIKNMMQALDNAGVQS---DIKVTTVVAMTVLEVSSPPSAGAFSAI 182
           EV+PG      +   +  +MQ+L N  V+S    I ++TVVAM+ LE S PPSAG F++ 
Sbjct: 128 EVIPGP-----IGPQVLPVMQSLTNL-VKSRNLPISISTVVAMSNLEQSYPPSAGMFTSQ 187

Query: 183 AARAMKDIGNVLESSCAPILVNVYPYFAYASNPQQISLSYALFTSTSPVVVDGDLQYFNL 242
           A   +  +  +L  +  PILVN+YPYFAYAS+P  I L YA F + S VV DG L Y N+
Sbjct: 188 AREQLVPVLKLLSQTSTPILVNIYPYFAYASDPANIRLDYASFNTKSIVVQDGSLGYSNM 247

Query: 243 LDAMVDSFYAALEKIGVEGVRIGISETGWPTKGNEPFTSVENALTYNKNFVEHVTSGVGT 302
            DA+ D+F  A+EK GV+ + + +SETGWP+ GN  FT+   A TYN+NFV+H+ SG GT
Sbjct: 248 FDAIFDAFVWAMEKEGVKNLPMVVSETGWPSAGNGNFTTPAIASTYNRNFVKHIASGKGT 307

Query: 303 PRMPNLQYDVVLFEMFNEDLKSPGVEQNFGFFDPS-MNPVYSFW 343
           P+ PN   +  LF  FNE+ K  G EQNFG ++PS M P+Y  +
Sbjct: 308 PKRPNKSMNGFLFATFNENQKPAGTEQNFGLYNPSDMKPIYKLF 345

[Close]

BLAST of Chy7G135200 vs. ExPASy Swiss-Prot
Match: O22317 (Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2)

HSP 1 Score: 285.8 bits (730), Expect = 6.5e-76
Identity = 144/312 (46.15%), Postives = 201/312 (64.42%), Query Frame = 0


Query: 28  AVGVNYGLNSDNLPKPNEVINLYERCGINIVRIFEPNHEILHALCGKENLVLWLGTRNED 87
           ++GV YG+  +NLP P+EV++LY+   I  +R+++PN   L AL    N+ + L     D
Sbjct: 28  SIGVCYGMLGNNLPPPSEVVSLYKSNNIARMRLYDPNQAALQAL-RNSNIQVLLDVPRSD 87

Query: 88  IEGFATNQEVANAWVNANVVPYYKDVNIAYITVGNEVVPGDTASPFVANAIKNMMQALDN 147
           ++  A+N   A  W+  NVV Y+  V+  YI VGNE++PG   + ++  A++N+  AL +
Sbjct: 88  VQSLASNPSAAGDWIRRNVVAYWPSVSFRYIAVGNELIPGSDLAQYILPAMRNIYNALSS 147

Query: 148 AGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIAARAMKDIGNVLESSCAPILVNVYPYFA 207
           AG+Q+ IKV+T V   VL  S PPSAGAFS+ A   +  I   L S+ AP+LVNVYPYF+
Sbjct: 148 AGLQNQIKVSTAVDTGVLGTSYPPSAGAFSSAAQAYLSPIVQFLASNGAPLLVNVYPYFS 207

Query: 208 YASNPQQISLSYALFTSTSPVVVDGDLQYFNLLDAMVDSFYAALEKIGVEGVRIGISETG 267
           Y  NP QISL YALFT++  VV DG   Y NL DA+VD+ +AALE++G   V + +SE+G
Sbjct: 208 YTGNPGQISLPYALFTASGVVVQDGRFSYQNLFDAIVDAVFAALERVGGANVAVVVSESG 267

Query: 268 WPTKGNEPFTSVENALTYNKNFVEHVTSGVGTPRMPNLQYDVVLFEMFNEDLKSPGVEQN 327
           WP+ G     S  NA TYN+N + HV  G GTPR P  + +  +FEMFNE+ K+ G+EQN
Sbjct: 268 WPSAGGGAEASTSNARTYNQNLIRHV--GGGTPRRPGKEIEAYIFEMFNENQKAGGIEQN 327

Query: 328 FGFFDPSMNPVY 340
           FG F P+  PVY
Sbjct: 328 FGLFYPNKQPVY 336

[Close]

BLAST of Chy7G135200 vs. ExPASy Swiss-Prot
Match: O49353 (Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana OX=3702 GN=BG5 PE=2 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 1.0e-73
Identity = 143/319 (44.83%), Postives = 208/319 (65.20%), Query Frame = 0


Query: 28  AVGVNYGLNSDNLPKPNEVINLYERCGINIVRIFEPNHEILHALCGKENLVLWLGTRNED 87
           ++G+NYGL  DNLP P++VI LY+   I  +RIF+PN E+L+AL G  ++ + +G R++D
Sbjct: 42  SIGLNYGLLGDNLPSPSKVITLYKSIDITKIRIFDPNTEVLNALRGHRDIAVTVGVRDQD 101

Query: 88  IEGFATNQEVANAWVNANVVPYYKDVNIAYITVGNEVVPGDTASPFVANAIKNMMQALDN 147
           +   + ++E    W   N+ PY  D+NIA+ITVGNEV+PG      +   +  +MQ+L N
Sbjct: 102 LAALSASEEAVKGWFATNIEPYLSDINIAFITVGNEVIPGP-----IGPQVLPVMQSLTN 161

Query: 148 AGVQS---DIKVTTVVAMTVLEVSSPPSAGAFSAIAARAMKDIGNVLESSCAPILVNVYP 207
             V+S    I ++TVVAM  LE S PPSAG F++ A   +  +  +L  + +PILV +YP
Sbjct: 162 L-VKSRNLPISISTVVAMWNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTNSPILVKIYP 221

Query: 208 YFAYASNPQQISLSYALFTSTSPVVVDGDLQYFNLLDAMVDSFYAALEKIGVEGVRIGIS 267
           YF+YAS+P  I L YA F + + VV DG L Y N+ DA+ D+F  A+EK GV+ + + +S
Sbjct: 222 YFSYASDPSSIRLDYATFNTEAIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKDLPMVVS 281

Query: 268 ETGWPTKGNEPFTSVENALTYNKNFVEHVTSGVGTPRMPNLQYDVVLFEMFNEDLKSPGV 327
           ETGWP+ GN   T+ + A TYN+NFV+H+ SG GTP+ PN   D  LF  FNE+ K  G 
Sbjct: 282 ETGWPSAGNGNITTPDIAGTYNRNFVKHIASGKGTPKRPNKGIDGFLFATFNENQKPVGT 341

Query: 328 EQNFGFFDPS-MNPVYSFW 343
           EQNFG ++P+ M P+Y+ +
Sbjct: 342 EQNFGLYNPNDMKPIYNLF 354

[Close]

BLAST of Chy7G135200 vs. ExPASy Swiss-Prot
Match: Q02439 (Putative glucan endo-1,3-beta-glucosidase GVI (Fragment) OS=Hordeum vulgare OX=4513 PE=3 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 4.4e-64
Identity = 140/313 (44.73%), Postives = 193/313 (61.66%), Query Frame = 0


Query: 29  VGVNYGLNSDNLPKPNEVINLYERCGINIVRIFEPNHEILHALCGKENLVLWLGTRNEDI 88
           +GVNYG+   +LP P++V+ LY+   I  VRIF P+  +L AL     L + LGT N D+
Sbjct: 7   IGVNYGMMGSDLPSPDKVVALYKANNITDVRIFHPDTNVLEAL-RNSGLGVVLGTLNSDL 66

Query: 89  EGFATNQEVANAWVNANVVPYYKDVNIAYITVGNEVVPGDTASPFVANAIKNMMQALDNA 148
              A++   A +WV++ V P+   V+  YI  GNEV+PG++A+  V  A+KN+  AL  A
Sbjct: 67  APLASDASYAASWVHSYVQPFAGAVSFRYINAGNEVIPGESAA-LVLPAMKNLEAALQAA 126

Query: 149 GVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIAARAMKDIGNVLESSCAPILVNVYPYFAY 208
           G+   + VTT +A +VL  S PPS G FS  A   +  I + L SS  P+LVNVYPYFAY
Sbjct: 127 GL--SVPVTTAMATSVLGTSYPPSQGTFSEAALPTVGPIVSHLASSGTPLLVNVYPYFAY 186

Query: 209 ASNPQQISLSYALFTSTSPV-VVDGDLQYFNLLDAMVDSFYAALEKI-GVEGVRIGISET 268
           +++P  + L YAL +S++ V V D  ++Y N+ DA++D+ YAA+EK  G E + + +SET
Sbjct: 187 SADPSSVRLDYALLSSSAAVAVTDNGVEYANMFDAILDAVYAAVEKAGGGESLELVVSET 246

Query: 269 GWPTKGNEPFTSVENALTYNKNFVEHVTSGVGTPRMPNLQYDVVLFEMFNEDLKSPGVEQ 328
           GWP+ G     SVENA  Y  N V HV    GTPR P    +  +F MFNE+ K  GVEQ
Sbjct: 247 GWPSGGGGYGASVENAAAYINNLVRHVG---GTPRRPGKAVETYIFAMFNENQKPEGVEQ 306

Query: 329 NFGFFDPSMNPVY 340
           NFG F P M+ VY
Sbjct: 307 NFGMFQPDMSQVY 312

[Close]

BLAST of Chy7G135200 vs. ExPASy Swiss-Prot
Match: P23431 (Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform OS=Nicotiana plumbaginifolia OX=4092 GN=GN2 PE=3 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 3.7e-63
Identity = 138/316 (43.67%), Postives = 195/316 (61.71%), Query Frame = 0


Query: 28  AVGVNYGLNSDNLPKPNEVINLYERCGINIVRIFEPNHEILHALCGKENLVLWLGTRNED 87
           ++GV YG+  +NLP   EVI LY+   I  +R+++PNH  L AL G  N+ + LG  N D
Sbjct: 34  SIGVCYGMLGNNLPNHWEVIQLYKSRNIGRLRLYDPNHGALQALKG-SNIEVMLGLPNSD 93

Query: 88  IEGFATNQEVANAWVNANVVPYYKDVNIAYITVGNEVVPGDTAS---PFVANAIKNMMQA 147
           ++  A+  E A  WV  NV  ++ DV I YI VGNE+ P    S    F+  A+ N+ +A
Sbjct: 94  VKHIASGMEHARWWVQKNVKDFWPDVKIKYIAVGNEISPVTGTSYLTSFLTPAMVNIYKA 153

Query: 148 LDNAGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIAARAMKDIGNVLESSCAPILVNVYP 207
           +  AG+ ++IKV+T V MT++  S PPS G+F   A   +  I   L  + AP+LVN+YP
Sbjct: 154 IGEAGLGNNIKVSTSVDMTLIGNSYPPSQGSFRNDARWFVDPIVGFLRDTRAPLLVNIYP 213

Query: 208 YFAYASNPQQISLSYALFTSTSPVVVDGDLQYFNLLDAMVDSFYAALEKIGVEGVRIGIS 267
           YF+Y+ NP QISL Y+LFT+ + VV DG  QY NL DAM+DS YAALE+ G   V I +S
Sbjct: 214 YFSYSGNPGQISLPYSLFTAPNVVVQDGSRQYRNLFDAMLDSVYAALERSGGASVGIVVS 273

Query: 268 ETGWPTKGNEPFTSVENALTYNKNFVEHVTSGVGTPRMPNLQYDVVLFEMFNEDLKSPGV 327
           E+GWP+ G    T  +NA TY KN ++H     G+PR P    +  +F MF+E+ K+P +
Sbjct: 274 ESGWPSAGAFGAT-YDNAATYLKNLIQHAKE--GSPRKPR-PIETYIFAMFDENNKNPEL 333

Query: 328 EQNFGFFDPSMNPVYS 341
           E++FG F P+  P Y+
Sbjct: 334 EKHFGLFSPNKQPKYN 344

[Close]

BLAST of Chy7G135200 vs. ExPASy TrEMBL
Match: A0A0A0L1Z1 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_4G377700 PE=3 SV=1)

HSP 1 Score: 683.7 bits (1763), Expect = 3.9e-193
Identity = 338/344 (98.26%), Postives = 340/344 (98.84%), Query Frame = 0


Query: 1   MPLFFPTTSLLFIFIFSHQFLHRSVFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRI 60
           MPLFFPTTSLLFIFIFSHQFLHRSVFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRI
Sbjct: 1   MPLFFPTTSLLFIFIFSHQFLHRSVFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRI 60

Query: 61  FEPNHEILHALCGKENLVLWLGTRNEDIEGFATNQEVANAWVNANVVPYYKDVNIAYITV 120
           FEPNHEILHALCGKENLVLWLGTRNEDIEGFATNQEVANAWVNANVV YYKDVNIAYITV
Sbjct: 61  FEPNHEILHALCGKENLVLWLGTRNEDIEGFATNQEVANAWVNANVVRYYKDVNIAYITV 120

Query: 121 GNEVVPGDTASPFVANAIKNMMQALDNAGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIA 180
           GNEVVPGD ASPFVANAIKNMMQALDNAGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIA
Sbjct: 121 GNEVVPGDAASPFVANAIKNMMQALDNAGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIA 180

Query: 181 ARAMKDIGNVLESSCAPILVNVYPYFAYASNPQQISLSYALFTSTSPVVVDGDLQYFNLL 240
           AR MKDIGNVLESSCAPILVNVYPYFAYASNPQQIS+SYALFTSTSPVVVDGDLQYFNLL
Sbjct: 181 ARTMKDIGNVLESSCAPILVNVYPYFAYASNPQQISMSYALFTSTSPVVVDGDLQYFNLL 240

Query: 241 DAMVDSFYAALEKIGVEGVRIGISETGWPTKGNEPFTSVENALTYNKNFVEHVTSGVGTP 300
           DAMVDSFYAALEKIGVEGVRIGISETGWPTKGNEPFTSVENALTYNKN VEHV+SGVGTP
Sbjct: 241 DAMVDSFYAALEKIGVEGVRIGISETGWPTKGNEPFTSVENALTYNKNIVEHVSSGVGTP 300

Query: 301 RMPNLQYDVVLFEMFNEDLKSPGVEQNFGFFDPSMNPVYSFWNC 345
           RMPNLQYDVVLFEMFNEDLKSPGVEQNFGFFDPSMNPVYSFWNC
Sbjct: 301 RMPNLQYDVVLFEMFNEDLKSPGVEQNFGFFDPSMNPVYSFWNC 344

[Close]

BLAST of Chy7G135200 vs. ExPASy TrEMBL
Match: A0A5A7V7B6 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold548G002240 PE=3 SV=1)

HSP 1 Score: 629.8 bits (1623), Expect = 6.8e-177
Identity = 310/344 (90.12%), Postives = 330/344 (95.93%), Query Frame = 0


Query: 1   MPLFFPTTSLLFIFIFSHQFLHRSVFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRI 60
           M LFFPTTSLL IFIFSHQ LH SVFGAVGVNYGLNSDNLPKP+EVINLYERCGINIVRI
Sbjct: 1   MALFFPTTSLLLIFIFSHQILHISVFGAVGVNYGLNSDNLPKPSEVINLYERCGINIVRI 60

Query: 61  FEPNHEILHALCGKENLVLWLGTRNEDIEGFATNQEVANAWVNANVVPYYKDVNIAYITV 120
           FEPNHE+L AL  KENLVLWLGTRNEDI+GFATNQEVA+AWVNANVVPYYKDVNIAYITV
Sbjct: 61  FEPNHEVLDALRCKENLVLWLGTRNEDIKGFATNQEVADAWVNANVVPYYKDVNIAYITV 120

Query: 121 GNEVVPGDTASPFVANAIKNMMQALDNAGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIA 180
           GNEVVPGD A+PFVANAI+N+MQAL NA VQSDIKVTTVVAMT LEVSSPPSAGAF+AIA
Sbjct: 121 GNEVVPGDAATPFVANAIQNIMQALVNADVQSDIKVTTVVAMTALEVSSPPSAGAFTAIA 180

Query: 181 ARAMKDIGNVLESSCAPILVNVYPYFAYASNPQQISLSYALFTSTSPVVVDGDLQYFNLL 240
           ARAMKDIG+VLESSCAPIL+NVYPYFAYASNPQQI LSYALFTST+PVVVDGDL+YFNLL
Sbjct: 181 ARAMKDIGSVLESSCAPILMNVYPYFAYASNPQQIPLSYALFTSTTPVVVDGDLEYFNLL 240

Query: 241 DAMVDSFYAALEKIGVEGVRIGISETGWPTKGNEPFTSVENALTYNKNFVEHVTSGVGTP 300
           DAMVDSFY ALEKIGVEGVRIGISETGWP+KGNEPFTSV+NALTYN+NFV+HV+SGVGTP
Sbjct: 241 DAMVDSFYTALEKIGVEGVRIGISETGWPSKGNEPFTSVDNALTYNQNFVKHVSSGVGTP 300

Query: 301 RMPNLQYDVVLFEMFNEDLKSPGVEQNFGFFDPSMNPVYSFWNC 345
           +MPNLQYD+VLFEMFNEDLKSPGVEQNFGFF P+MNPVYSFWNC
Sbjct: 301 KMPNLQYDIVLFEMFNEDLKSPGVEQNFGFFYPNMNPVYSFWNC 344

[Close]

BLAST of Chy7G135200 vs. ExPASy TrEMBL
Match: A0A5D3D504 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold119G001690 PE=3 SV=1)

HSP 1 Score: 629.8 bits (1623), Expect = 6.8e-177
Identity = 310/344 (90.12%), Postives = 330/344 (95.93%), Query Frame = 0


Query: 1   MPLFFPTTSLLFIFIFSHQFLHRSVFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRI 60
           M LFFPTTSLL IFIFSHQ LH SVFGAVGVNYGLNSDNLPKP+EVINLYERCGINIVRI
Sbjct: 1   MALFFPTTSLLLIFIFSHQILHISVFGAVGVNYGLNSDNLPKPSEVINLYERCGINIVRI 60

Query: 61  FEPNHEILHALCGKENLVLWLGTRNEDIEGFATNQEVANAWVNANVVPYYKDVNIAYITV 120
           FEPNHE+L AL  KENLVLWLGTRNEDI+GFATNQEVA+AWVNANVVPYYKDVNIAYITV
Sbjct: 61  FEPNHEVLDALRCKENLVLWLGTRNEDIKGFATNQEVADAWVNANVVPYYKDVNIAYITV 120

Query: 121 GNEVVPGDTASPFVANAIKNMMQALDNAGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIA 180
           GNEVVPGD A+PFVANAI+N+MQAL NA VQSDIKVTTVVAMT LEVSSPPSAGAF+AIA
Sbjct: 121 GNEVVPGDAATPFVANAIQNIMQALVNADVQSDIKVTTVVAMTALEVSSPPSAGAFTAIA 180

Query: 181 ARAMKDIGNVLESSCAPILVNVYPYFAYASNPQQISLSYALFTSTSPVVVDGDLQYFNLL 240
           ARAMKDIG+VLESSCAPIL+NVYPYFAYASNPQQI LSYALFTST+PVVVDGDL+YFNLL
Sbjct: 181 ARAMKDIGSVLESSCAPILMNVYPYFAYASNPQQIPLSYALFTSTTPVVVDGDLEYFNLL 240

Query: 241 DAMVDSFYAALEKIGVEGVRIGISETGWPTKGNEPFTSVENALTYNKNFVEHVTSGVGTP 300
           DAMVDSFY ALEKIGVEGVRIGISETGWP+KGNEPFTSV+NALTYN+NFV+HV+SGVGTP
Sbjct: 241 DAMVDSFYTALEKIGVEGVRIGISETGWPSKGNEPFTSVDNALTYNQNFVKHVSSGVGTP 300

Query: 301 RMPNLQYDVVLFEMFNEDLKSPGVEQNFGFFDPSMNPVYSFWNC 345
           +MPNLQYD+VLFEMFNEDLKSPGVEQNFGFF P+MNPVYSFWNC
Sbjct: 301 KMPNLQYDIVLFEMFNEDLKSPGVEQNFGFFYPNMNPVYSFWNC 344

[Close]

BLAST of Chy7G135200 vs. ExPASy TrEMBL
Match: A0A1S4DV56 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103487671 PE=3 SV=1)

HSP 1 Score: 560.8 bits (1444), Expect = 3.9e-156
Identity = 279/314 (88.85%), Postives = 299/314 (95.22%), Query Frame = 0


Query: 1   MPLFFPTTSLLFIFIFSHQFLHRSVFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRI 60
           M LFFPTTSLL IFIFSHQ LH SVFGAVGVNYGLNSDNLPKP+EVINLYERCGINIVRI
Sbjct: 1   MALFFPTTSLLLIFIFSHQILHISVFGAVGVNYGLNSDNLPKPSEVINLYERCGINIVRI 60

Query: 61  FEPNHEILHALCGKENLVLWLGTRNEDIEGFATNQEVANAWVNANVVPYYKDVNIAYITV 120
           FEPNHE+L AL  KENLVLWLGTRNEDI+GFATNQEVA+AWVNANVVPYYKDVNIAYITV
Sbjct: 61  FEPNHEVLDALRCKENLVLWLGTRNEDIKGFATNQEVADAWVNANVVPYYKDVNIAYITV 120

Query: 121 GNEVVPGDTASPFVANAIKNMMQALDNAGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIA 180
           GNEVVPGD A+PFVANAI+N+MQAL NA VQSDIKVTTVVAMT LEVSSPPSAGAF+AIA
Sbjct: 121 GNEVVPGDAATPFVANAIQNIMQALVNADVQSDIKVTTVVAMTALEVSSPPSAGAFTAIA 180

Query: 181 ARAMKDIGNVLESSCAPILVNVYPYFAYASNPQQISLSYALFTSTSPVVVDGDLQYFNLL 240
           ARAMKDIG+VLESSCAPIL+NVYPYFAYASNPQQI LSYALFTST+PVVVDGDL+YFNLL
Sbjct: 181 ARAMKDIGSVLESSCAPILMNVYPYFAYASNPQQIPLSYALFTSTTPVVVDGDLEYFNLL 240

Query: 241 DAMVDSFYAALEKIGVEGVRIGISETGWPTKGNEPFTSVENALTYNKNFVEHVTSGVGTP 300
           DAMVDSFY ALEKIGVEGVRIGISETGWP+KGNEPFTSV+NALTYN+NFV+HV+SGVGTP
Sbjct: 241 DAMVDSFYTALEKIGVEGVRIGISETGWPSKGNEPFTSVDNALTYNQNFVKHVSSGVGTP 300

Query: 301 RMPNLQYDVVLFEM 315
           +MPNLQYD+V F +
Sbjct: 301 KMPNLQYDIVFFSI 314

[Close]

BLAST of Chy7G135200 vs. ExPASy TrEMBL
Match: A0A1S4DV56 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103487671 PE=3 SV=1)

HSP 1 Score: 542.0 bits (1395), Expect = 1.9e-150
Identity = 269/335 (80.30%), Postives = 291/335 (86.87%), Query Frame = 0


Query: 10  LLFIFIFSHQFLHRSVFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRIFEPNHEILH 69
           LLF+      FL     G +GVNYGLN DNLP P++VINLY RCGINIVR+FEPNH +L 
Sbjct: 318 LLFLCFNVLNFLFPGGSGTIGVNYGLNGDNLPIPSDVINLYRRCGINIVRLFEPNHGVLD 377

Query: 70  ALCGKENLVLWLGTRNEDIEGFATNQEVANAWVNANVVPYYKDVNIAYITVGNEVVPGDT 129
           AL GK++LVLWLGTRNEDIEGFATNQ  AN WVNANVVPYYKDVNI+YITVGNEVVPGD 
Sbjct: 378 ALQGKKDLVLWLGTRNEDIEGFATNQLAANTWVNANVVPYYKDVNISYITVGNEVVPGDA 437

Query: 130 ASPFVANAIKNMMQALDNAGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIAARAMKDIGN 189
           A+PFVANAIKN+MQAL N G++SDIKVTTVVAMT LEVS PPSAGAF+  AA  MKDI N
Sbjct: 438 AAPFVANAIKNIMQALVNIGIKSDIKVTTVVAMTGLEVSYPPSAGAFTDSAAGIMKDIAN 497

Query: 190 VLESSCAPILVNVYPYFAYASNPQQISLSYALFTSTSPVVVDGDLQYFNLLDAMVDSFYA 249
           VL SS APILVNVYPYFAYASNPQQISL+YALFTST+PVVVDGDLQYFNL DAMVDSFYA
Sbjct: 498 VLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLFDAMVDSFYA 557

Query: 250 ALEKIGVEGVRIGISETGWPTKGNEPFTSVENALTYNKNFVEHVTSGVGTPRMPNLQYDV 309
           ALEKI  E +RIGISETGWPT GNEPFTSV+NALTYNKN V HVTSGVGTP+ PNL+YD 
Sbjct: 558 ALEKIDAEEIRIGISETGWPTSGNEPFTSVDNALTYNKNLVNHVTSGVGTPKRPNLKYDA 617

Query: 310 VLFEMFNEDLKSPGVEQNFGFFDPSMNPVYSFWNC 345
           VLFEMFNEDLK+PGVEQNFGFF P+MNPVY FWNC
Sbjct: 618 VLFEMFNEDLKAPGVEQNFGFFFPNMNPVYPFWNC 652

HSP 2 Score: 557.8 bits (1436), Expect = 3.3e-155
Identity = 276/344 (80.23%), Postives = 300/344 (87.21%), Query Frame = 0


Query: 1   MPLFFPTTSLLFIFIFSHQFLHRSVFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRI 60
           M  FFPT+ LL + +FSHQ    S  G +GVNYGLN DNLP P++VINLY RCGINIVR+
Sbjct: 1   MDFFFPTSFLLLLCLFSHQIPPISGSGTIGVNYGLNGDNLPIPSDVINLYRRCGINIVRL 60

Query: 61  FEPNHEILHALCGKENLVLWLGTRNEDIEGFATNQEVANAWVNANVVPYYKDVNIAYITV 120
           FEPNH +L AL GK++LVLWLGTRNEDIEGFATNQ  AN WVNANVVPYYKDVNI+YITV
Sbjct: 61  FEPNHGVLDALQGKKDLVLWLGTRNEDIEGFATNQLAANTWVNANVVPYYKDVNISYITV 120

Query: 121 GNEVVPGDTASPFVANAIKNMMQALDNAGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIA 180
           GNEVVPGD A+PFVANAIKN+MQAL N G++SDIKVTTVVAMT LEVS PPSAGAF+  A
Sbjct: 121 GNEVVPGDAAAPFVANAIKNIMQALVNIGIKSDIKVTTVVAMTGLEVSYPPSAGAFTDSA 180

Query: 181 ARAMKDIGNVLESSCAPILVNVYPYFAYASNPQQISLSYALFTSTSPVVVDGDLQYFNLL 240
           A  MKDI NVL SS APILVNVYPYFAYASNPQQISL+YALFTST+PVVVDGDLQYFNL 
Sbjct: 181 AGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLF 240

Query: 241 DAMVDSFYAALEKIGVEGVRIGISETGWPTKGNEPFTSVENALTYNKNFVEHVTSGVGTP 300
           DAMVDSFYAALEKI  E +RIGISETGWPT GNEPFTSV+NALTYNKN V HVTSGVGTP
Sbjct: 241 DAMVDSFYAALEKIDAEEIRIGISETGWPTSGNEPFTSVDNALTYNKNLVNHVTSGVGTP 300

Query: 301 RMPNLQYDVVLFEMFNEDLKSPGVEQNFGFFDPSMNPVYSFWNC 345
           + PNL+YD VLFEMFNEDLK+PGVEQNFGFF P+MNPVY FWNC
Sbjct: 301 KRPNLKYDAVLFEMFNEDLKAPGVEQNFGFFFPNMNPVYPFWNC 344

[Close]

BLAST of Chy7G135200 vs. NCBI nr
Match: XP_031740439.1 (glucan endo-1,3-beta-glucosidase [Cucumis sativus])

HSP 1 Score: 680 bits (1754), Expect = 1.93e-246
Identity = 338/344 (98.26%), Postives = 340/344 (98.84%), Query Frame = 0


Query: 1   MPLFFPTTSLLFIFIFSHQFLHRSVFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRI 60
           MPLFFPTTSLLFIFIFSHQFLHRSVFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRI
Sbjct: 1   MPLFFPTTSLLFIFIFSHQFLHRSVFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRI 60

Query: 61  FEPNHEILHALCGKENLVLWLGTRNEDIEGFATNQEVANAWVNANVVPYYKDVNIAYITV 120
           FEPNHEILHALCGKENLVLWLGTRNEDIEGFATNQEVANAWVNANVV YYKDVNIAYITV
Sbjct: 61  FEPNHEILHALCGKENLVLWLGTRNEDIEGFATNQEVANAWVNANVVRYYKDVNIAYITV 120

Query: 121 GNEVVPGDTASPFVANAIKNMMQALDNAGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIA 180
           GNEVVPGD ASPFVANAIKNMMQALDNAGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIA
Sbjct: 121 GNEVVPGDAASPFVANAIKNMMQALDNAGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIA 180

Query: 181 ARAMKDIGNVLESSCAPILVNVYPYFAYASNPQQISLSYALFTSTSPVVVDGDLQYFNLL 240
           AR MKDIGNVLESSCAPILVNVYPYFAYASNPQQIS+SYALFTSTSPVVVDGDLQYFNLL
Sbjct: 181 ARTMKDIGNVLESSCAPILVNVYPYFAYASNPQQISMSYALFTSTSPVVVDGDLQYFNLL 240

Query: 241 DAMVDSFYAALEKIGVEGVRIGISETGWPTKGNEPFTSVENALTYNKNFVEHVTSGVGTP 300
           DAMVDSFYAALEKIGVEGVRIGISETGWPTKGNEPFTSVENALTYNKN VEHV+SGVGTP
Sbjct: 241 DAMVDSFYAALEKIGVEGVRIGISETGWPTKGNEPFTSVENALTYNKNIVEHVSSGVGTP 300

Query: 301 RMPNLQYDVVLFEMFNEDLKSPGVEQNFGFFDPSMNPVYSFWNC 344
           RMPNLQYDVVLFEMFNEDLKSPGVEQNFGFFDPSMNPVYSFWNC
Sbjct: 301 RMPNLQYDVVLFEMFNEDLKSPGVEQNFGFFDPSMNPVYSFWNC 344

[Close]

BLAST of Chy7G135200 vs. NCBI nr
Match: KAE8649635.1 (hypothetical protein Csa_012736 [Cucumis sativus])

HSP 1 Score: 654 bits (1686), Expect = 3.19e-231
Identity = 328/334 (98.20%), Postives = 330/334 (98.80%), Query Frame = 0


Query: 1   MPLFFPTTSLLFIFIFSHQFLHRSVFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRI 60
           MPLFFPTTSLLFIFIFSHQFLHRSVFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRI
Sbjct: 1   MPLFFPTTSLLFIFIFSHQFLHRSVFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRI 60

Query: 61  FEPNHEILHALCGKENLVLWLGTRNEDIEGFATNQEVANAWVNANVVPYYKDVNIAYITV 120
           FEPNHEILHALCGKENLVLWLGTRNEDIEGFATNQEVANAWVNANVV YYKDVNIAYITV
Sbjct: 61  FEPNHEILHALCGKENLVLWLGTRNEDIEGFATNQEVANAWVNANVVRYYKDVNIAYITV 120

Query: 121 GNEVVPGDTASPFVANAIKNMMQALDNAGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIA 180
           GNEVVPGD ASPFVANAIKNMMQALDNAGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIA
Sbjct: 121 GNEVVPGDAASPFVANAIKNMMQALDNAGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIA 180

Query: 181 ARAMKDIGNVLESSCAPILVNVYPYFAYASNPQQISLSYALFTSTSPVVVDGDLQYFNLL 240
           AR MKDIGNVLESSCAPILVNVYPYFAYASNPQQIS+SYALFTSTSPVVVDGDLQYFNLL
Sbjct: 181 ARTMKDIGNVLESSCAPILVNVYPYFAYASNPQQISMSYALFTSTSPVVVDGDLQYFNLL 240

Query: 241 DAMVDSFYAALEKIGVEGVRIGISETGWPTKGNEPFTSVENALTYNKNFVEHVTSGVGTP 300
           DAMVDSFYAALEKIGVEGVRIGISETGWPTKGNEPFTSVENALTYNKN VEHV+SGVGTP
Sbjct: 241 DAMVDSFYAALEKIGVEGVRIGISETGWPTKGNEPFTSVENALTYNKNIVEHVSSGVGTP 300

Query: 301 RMPNLQYDVVLFEMFNEDLKSPGVEQNFGFFDPS 334
           RMPNLQYDVVLFEMFNEDLKSPGVEQNFGFFDPS
Sbjct: 301 RMPNLQYDVVLFEMFNEDLKSPGVEQNFGFFDPS 334

[Close]

BLAST of Chy7G135200 vs. NCBI nr
Match: TYK18631.1 (putative glucan endo-1,3-beta-glucosidase BG4 [Cucumis melo var. makuwa])

HSP 1 Score: 626 bits (1614), Expect = 4.19e-225
Identity = 310/344 (90.12%), Postives = 330/344 (95.93%), Query Frame = 0


Query: 1   MPLFFPTTSLLFIFIFSHQFLHRSVFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRI 60
           M LFFPTTSLL IFIFSHQ LH SVFGAVGVNYGLNSDNLPKP+EVINLYERCGINIVRI
Sbjct: 1   MALFFPTTSLLLIFIFSHQILHISVFGAVGVNYGLNSDNLPKPSEVINLYERCGINIVRI 60

Query: 61  FEPNHEILHALCGKENLVLWLGTRNEDIEGFATNQEVANAWVNANVVPYYKDVNIAYITV 120
           FEPNHE+L AL  KENLVLWLGTRNEDI+GFATNQEVA+AWVNANVVPYYKDVNIAYITV
Sbjct: 61  FEPNHEVLDALRCKENLVLWLGTRNEDIKGFATNQEVADAWVNANVVPYYKDVNIAYITV 120

Query: 121 GNEVVPGDTASPFVANAIKNMMQALDNAGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIA 180
           GNEVVPGD A+PFVANAI+N+MQAL NA VQSDIKVTTVVAMT LEVSSPPSAGAF+AIA
Sbjct: 121 GNEVVPGDAATPFVANAIQNIMQALVNADVQSDIKVTTVVAMTALEVSSPPSAGAFTAIA 180

Query: 181 ARAMKDIGNVLESSCAPILVNVYPYFAYASNPQQISLSYALFTSTSPVVVDGDLQYFNLL 240
           ARAMKDIG+VLESSCAPIL+NVYPYFAYASNPQQI LSYALFTST+PVVVDGDL+YFNLL
Sbjct: 181 ARAMKDIGSVLESSCAPILMNVYPYFAYASNPQQIPLSYALFTSTTPVVVDGDLEYFNLL 240

Query: 241 DAMVDSFYAALEKIGVEGVRIGISETGWPTKGNEPFTSVENALTYNKNFVEHVTSGVGTP 300
           DAMVDSFY ALEKIGVEGVRIGISETGWP+KGNEPFTSV+NALTYN+NFV+HV+SGVGTP
Sbjct: 241 DAMVDSFYTALEKIGVEGVRIGISETGWPSKGNEPFTSVDNALTYNQNFVKHVSSGVGTP 300

Query: 301 RMPNLQYDVVLFEMFNEDLKSPGVEQNFGFFDPSMNPVYSFWNC 344
           +MPNLQYD+VLFEMFNEDLKSPGVEQNFGFF P+MNPVYSFWNC
Sbjct: 301 KMPNLQYDIVLFEMFNEDLKSPGVEQNFGFFYPNMNPVYSFWNC 344

[Close]

BLAST of Chy7G135200 vs. NCBI nr
Match: KAA0064262.1 (putative glucan endo-1,3-beta-glucosidase BG4 [Cucumis melo var. makuwa])

HSP 1 Score: 626 bits (1614), Expect = 3.85e-220
Identity = 310/344 (90.12%), Postives = 330/344 (95.93%), Query Frame = 0


Query: 1   MPLFFPTTSLLFIFIFSHQFLHRSVFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRI 60
           M LFFPTTSLL IFIFSHQ LH SVFGAVGVNYGLNSDNLPKP+EVINLYERCGINIVRI
Sbjct: 1   MALFFPTTSLLLIFIFSHQILHISVFGAVGVNYGLNSDNLPKPSEVINLYERCGINIVRI 60

Query: 61  FEPNHEILHALCGKENLVLWLGTRNEDIEGFATNQEVANAWVNANVVPYYKDVNIAYITV 120
           FEPNHE+L AL  KENLVLWLGTRNEDI+GFATNQEVA+AWVNANVVPYYKDVNIAYITV
Sbjct: 61  FEPNHEVLDALRCKENLVLWLGTRNEDIKGFATNQEVADAWVNANVVPYYKDVNIAYITV 120

Query: 121 GNEVVPGDTASPFVANAIKNMMQALDNAGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIA 180
           GNEVVPGD A+PFVANAI+N+MQAL NA VQSDIKVTTVVAMT LEVSSPPSAGAF+AIA
Sbjct: 121 GNEVVPGDAATPFVANAIQNIMQALVNADVQSDIKVTTVVAMTALEVSSPPSAGAFTAIA 180

Query: 181 ARAMKDIGNVLESSCAPILVNVYPYFAYASNPQQISLSYALFTSTSPVVVDGDLQYFNLL 240
           ARAMKDIG+VLESSCAPIL+NVYPYFAYASNPQQI LSYALFTST+PVVVDGDL+YFNLL
Sbjct: 181 ARAMKDIGSVLESSCAPILMNVYPYFAYASNPQQIPLSYALFTSTTPVVVDGDLEYFNLL 240

Query: 241 DAMVDSFYAALEKIGVEGVRIGISETGWPTKGNEPFTSVENALTYNKNFVEHVTSGVGTP 300
           DAMVDSFY ALEKIGVEGVRIGISETGWP+KGNEPFTSV+NALTYN+NFV+HV+SGVGTP
Sbjct: 241 DAMVDSFYTALEKIGVEGVRIGISETGWPSKGNEPFTSVDNALTYNQNFVKHVSSGVGTP 300

Query: 301 RMPNLQYDVVLFEMFNEDLKSPGVEQNFGFFDPSMNPVYSFWNC 344
           +MPNLQYD+VLFEMFNEDLKSPGVEQNFGFF P+MNPVYSFWNC
Sbjct: 301 KMPNLQYDIVLFEMFNEDLKSPGVEQNFGFFYPNMNPVYSFWNC 344

[Close]

BLAST of Chy7G135200 vs. NCBI nr
Match: TYK18632.1 (putative glucan endo-1,3-beta-glucosidase BG4 [Cucumis melo var. makuwa])

HSP 1 Score: 554 bits (1427), Expect = 1.28e-196
Identity = 276/344 (80.23%), Postives = 300/344 (87.21%), Query Frame = 0


Query: 1   MPLFFPTTSLLFIFIFSHQFLHRSVFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRI 60
           M  FFPT+ LL + +FSHQ    S  G +GVNYGLN DNLP P++VINLY RCGINIVR+
Sbjct: 1   MDFFFPTSFLLLLCLFSHQIPPISGSGTIGVNYGLNGDNLPIPSDVINLYRRCGINIVRL 60

Query: 61  FEPNHEILHALCGKENLVLWLGTRNEDIEGFATNQEVANAWVNANVVPYYKDVNIAYITV 120
           FEPNH +L AL GK++LVLWLGTRNEDIEGFATNQ  AN WVNANVVPYYKDVNI+YITV
Sbjct: 61  FEPNHGVLDALQGKKDLVLWLGTRNEDIEGFATNQLAANTWVNANVVPYYKDVNISYITV 120

Query: 121 GNEVVPGDTASPFVANAIKNMMQALDNAGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIA 180
           GNEVVPGD A+PFVANAIKN+MQAL N G++SDIKVTTVVAMT LEVS PPSAGAF+  A
Sbjct: 121 GNEVVPGDAAAPFVANAIKNIMQALVNIGIKSDIKVTTVVAMTGLEVSYPPSAGAFTDSA 180

Query: 181 ARAMKDIGNVLESSCAPILVNVYPYFAYASNPQQISLSYALFTSTSPVVVDGDLQYFNLL 240
           A  MKDI NVL SS APILVNVYPYFAYASNPQQISL+YALFTST+PVVVDGDLQYFNL 
Sbjct: 181 AGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLF 240

Query: 241 DAMVDSFYAALEKIGVEGVRIGISETGWPTKGNEPFTSVENALTYNKNFVEHVTSGVGTP 300
           DAMVDSFYAALEKI  E +RIGISETGWPT GNEPFTSV+NALTYNKN V HVTSGVGTP
Sbjct: 241 DAMVDSFYAALEKIDAEEIRIGISETGWPTSGNEPFTSVDNALTYNKNLVNHVTSGVGTP 300

Query: 301 RMPNLQYDVVLFEMFNEDLKSPGVEQNFGFFDPSMNPVYSFWNC 344
           + PNL+YD VLFEMFNEDLK+PGVEQNFGFF P+MNPVY FWNC
Sbjct: 301 KRPNLKYDAVLFEMFNEDLKAPGVEQNFGFFFPNMNPVYPFWNC 344

[Close]

BLAST of Chy7G135200 vs. TAIR 10
Match: AT1G77780.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 293.5 bits (750), Expect = 2.2e-79
Identity = 150/314 (47.77%), Postives = 206/314 (65.61%), Query Frame = 0


Query: 30  GVNYGLNSDNLPKPNEVINLYERCGINIVRIFEPNHEILHALCGKENLVLWLGTRNEDIE 89
           GV YG N DNLP P + ++LY++  +  +R++EP  +++ +L G   L++ +G RNE I+
Sbjct: 25  GVCYGRNGDNLPSPAKTVSLYKKINVGGIRLYEPFPDLIVSLQG-TGLLVAIGPRNEAIK 84

Query: 90  GFATNQEVANAWVNANVVPYYKDVNIAYITVGNEVVPGDTASPFVANAIKNMMQALDNAG 149
             A   + A  W    + P YK+V   +ITVGNEV+ G+    +V  A+KN+  AL   G
Sbjct: 85  TLAEEYQFALNWDKTFIAP-YKNVAFNWITVGNEVIEGEIGR-YVPQAMKNIKAALTEIG 144

Query: 150 VQSDIKVTTVVAMTVLEVSSPPSAGAFSAIAARAMKDIGNVLESSCAPILVNVYPYFAYA 209
             S I VTTV++   L  S PPSAG F       + +I ++L S+ +P++VNVYPYFAYA
Sbjct: 145 -NSKIHVTTVISTAALANSYPPSAGVFKPAITELITEIVSILSSTDSPLMVNVYPYFAYA 204

Query: 210 SNPQQISLSYALFTSTSPVVVDGDLQYFNLLDAMVDSFYAALEKIGVEGVRIGISETGWP 269
           S+P  +SL YA F STSPVV DG  QY N+ DA +D+F  ALEKI    V++ ++ETGWP
Sbjct: 205 SDPSHVSLEYATFRSTSPVVTDGKYQYTNIFDATLDAFNVALEKINHGSVKVYVAETGWP 264

Query: 270 TKGNEPFTSVENALTYNKNFVEHVTSGVGTPRMPNLQYDVVLFEMFNEDLKSPGVEQNFG 329
           T+GN+P+TSVENA  YN+  ++ +T+G GTPR PN+      FEMFNEDLK   VEQ+FG
Sbjct: 265 TRGNDPYTSVENARAYNQGLLKKLTTGKGTPRRPNVPVITFFFEMFNEDLKQGAVEQSFG 324

Query: 330 FFDPSMNPVYSFWN 344
           FFDP+M PVY  WN
Sbjct: 325 FFDPNMAPVYDMWN 334

[Close]

BLAST of Chy7G135200 vs. TAIR 10
Match: AT5G20330.1 (beta-1,3-glucanase 4 )

HSP 1 Score: 286.6 bits (732), Expect = 2.7e-77
Identity = 153/344 (44.48%), Postives = 219/344 (63.66%), Query Frame = 0


Query: 3   LFFPTTSLLFIFIFSHQFLHRSVFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRIFE 62
           LF    S + +++ S+     +   ++G+NYGL  DNLP P+ VINLY+  GI+ +RIF+
Sbjct: 8   LFLFFLSCIVLYVNSNNSGFVTAANSIGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFD 67

Query: 63  PNHEILHALCGKENLVLWLGTRNEDIEGFATNQEVANAWVNANVVPYYKDVNIAYITVGN 122
           PN E+L+AL G  ++ + +G +++D+   A ++E    W  AN+  Y  DVNI +ITVGN
Sbjct: 68  PNTEVLNALRGHRDIEVTVGVKDQDLAALAASEEAVKGWFAANIESYLADVNITFITVGN 127

Query: 123 EVVPGDTASPFVANAIKNMMQALDNAGVQS---DIKVTTVVAMTVLEVSSPPSAGAFSAI 182
           EV+PG      +   +  +MQ+L N  V+S    I ++TVVAM+ LE S PPSAG F++ 
Sbjct: 128 EVIPGP-----IGPQVLPVMQSLTNL-VKSRNLPISISTVVAMSNLEQSYPPSAGMFTSQ 187

Query: 183 AARAMKDIGNVLESSCAPILVNVYPYFAYASNPQQISLSYALFTSTSPVVVDGDLQYFNL 242
           A   +  +  +L  +  PILVN+YPYFAYAS+P  I L YA F + S VV DG L Y N+
Sbjct: 188 AREQLVPVLKLLSQTSTPILVNIYPYFAYASDPANIRLDYASFNTKSIVVQDGSLGYSNM 247

Query: 243 LDAMVDSFYAALEKIGVEGVRIGISETGWPTKGNEPFTSVENALTYNKNFVEHVTSGVGT 302
            DA+ D+F  A+EK GV+ + + +SETGWP+ GN  FT+   A TYN+NFV+H+ SG GT
Sbjct: 248 FDAIFDAFVWAMEKEGVKNLPMVVSETGWPSAGNGNFTTPAIASTYNRNFVKHIASGKGT 307

Query: 303 PRMPNLQYDVVLFEMFNEDLKSPGVEQNFGFFDPS-MNPVYSFW 343
           P+ PN   +  LF  FNE+ K  G EQNFG ++PS M P+Y  +
Sbjct: 308 PKRPNKSMNGFLFATFNENQKPAGTEQNFGLYNPSDMKPIYKLF 345

[Close]

BLAST of Chy7G135200 vs. TAIR 10
Match: AT5G20340.1 (beta-1,3-glucanase 5 )

HSP 1 Score: 278.5 bits (711), Expect = 7.4e-75
Identity = 143/319 (44.83%), Postives = 208/319 (65.20%), Query Frame = 0


Query: 28  AVGVNYGLNSDNLPKPNEVINLYERCGINIVRIFEPNHEILHALCGKENLVLWLGTRNED 87
           ++G+NYGL  DNLP P++VI LY+   I  +RIF+PN E+L+AL G  ++ + +G R++D
Sbjct: 42  SIGLNYGLLGDNLPSPSKVITLYKSIDITKIRIFDPNTEVLNALRGHRDIAVTVGVRDQD 101

Query: 88  IEGFATNQEVANAWVNANVVPYYKDVNIAYITVGNEVVPGDTASPFVANAIKNMMQALDN 147
           +   + ++E    W   N+ PY  D+NIA+ITVGNEV+PG      +   +  +MQ+L N
Sbjct: 102 LAALSASEEAVKGWFATNIEPYLSDINIAFITVGNEVIPGP-----IGPQVLPVMQSLTN 161

Query: 148 AGVQS---DIKVTTVVAMTVLEVSSPPSAGAFSAIAARAMKDIGNVLESSCAPILVNVYP 207
             V+S    I ++TVVAM  LE S PPSAG F++ A   +  +  +L  + +PILV +YP
Sbjct: 162 L-VKSRNLPISISTVVAMWNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTNSPILVKIYP 221

Query: 208 YFAYASNPQQISLSYALFTSTSPVVVDGDLQYFNLLDAMVDSFYAALEKIGVEGVRIGIS 267
           YF+YAS+P  I L YA F + + VV DG L Y N+ DA+ D+F  A+EK GV+ + + +S
Sbjct: 222 YFSYASDPSSIRLDYATFNTEAIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKDLPMVVS 281

Query: 268 ETGWPTKGNEPFTSVENALTYNKNFVEHVTSGVGTPRMPNLQYDVVLFEMFNEDLKSPGV 327
           ETGWP+ GN   T+ + A TYN+NFV+H+ SG GTP+ PN   D  LF  FNE+ K  G 
Sbjct: 282 ETGWPSAGNGNITTPDIAGTYNRNFVKHIASGKGTPKRPNKGIDGFLFATFNENQKPVGT 341

Query: 328 EQNFGFFDPS-MNPVYSFW 343
           EQNFG ++P+ M P+Y+ +
Sbjct: 342 EQNFGLYNPNDMKPIYNLF 354

[Close]

BLAST of Chy7G135200 vs. TAIR 10
Match: AT1G77790.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 269.6 bits (688), Expect = 3.4e-72
Identity = 148/312 (47.44%), Postives = 194/312 (62.18%), Query Frame = 0


Query: 33  YGLNSDNLPKPNEVINLYERCGINIVRIFEPNHEILHALCGKENLVLWLGTRNEDIEGFA 92
           YG N +NLP P + + LY+   I+ +R++EP  ++L AL G   L++  G RNEDI+  A
Sbjct: 34  YGRNGNNLPTPADTVALYKSNNIDAIRMYEPFADMLEALRG-SGLLVAFGPRNEDIQSLA 93

Query: 93  TNQEVANAWVNANVVPYYKDVNIAYITVGNEVVPGDTASPFVANAIKNMMQALDNAGVQS 152
            +   A  +V+  + PY  DV I +IT+GNEV PG+ A  FVA AIKN+  AL N+GV +
Sbjct: 94  HDPAAATNFVSTWITPYQNDVAIKWITIGNEVFPGEIAQ-FVAAAIKNVNVALTNSGV-T 153

Query: 153 DIKVTTVVAMTVLEVSSPPSAGAFSAIAARAMKDIGNVLESSCAPILVNVYPYFAYASNP 212
            I VTTV+AMT L  + PPSA  F       M +I ++L  + +P++ N+YPYFAYAS+P
Sbjct: 154 GISVTTVLAMTALTNTYPPSAATFLPDLTEIMTEITSILSETNSPLMTNIYPYFAYASDP 213

Query: 213 QQISLSYALFTSTSPVVVDGDLQYFNLLDAMVDSFYAALEKIGVEGVRIGISETGWPTKG 272
             ISL YA F S +PVV+DGDL Y N+ +AMVD F AALEKI    V + ++ETGWPT+G
Sbjct: 214 YHISLDYASFKSNTPVVIDGDLYYNNMFEAMVDGFNAALEKINAANVVVMVAETGWPTEG 273

Query: 273 NEPFTSVENALTYNKNF--VEHVTSGVGTPRMPNLQYDVVLFEMFNEDLKSPGVEQNFGF 332
           N P TSV+NA  YN              TPR  N   DV LF MF E+ K   VEQ+FG 
Sbjct: 274 NPPHTSVDNAKAYNMGIRTCGRSAERKRTPRRQNTPVDVFLFAMFKENQKDGPVEQSFGI 333

Query: 333 FDPSMNPVYSFW 343
           F P M PVY  +
Sbjct: 334 FAPDMTPVYDLF 342

[Close]

BLAST of Chy7G135200 vs. TAIR 10
Match: AT5G20560.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 265.8 bits (678), Expect = 4.9e-71
Identity = 135/331 (40.79%), Postives = 201/331 (60.73%), Query Frame = 0


Query: 3   LFFPTTSLLFIFIFSHQFLHRSVFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRIFE 62
           L F  T+L+  +   +     S    VG+NYGL  DNLP P+ VI  Y+   +  +RIFE
Sbjct: 11  LLFSCTALIISY---YNVDSLSTASVVGLNYGLLGDNLPSPSNVIKFYKSQNVAKIRIFE 70

Query: 63  PNHEILHALCGKENLVLWLGTRNEDIEGFATNQEVANAWVNANVVPYYKDVNIAYITVGN 122
           PN ++L+AL G  ++ + +G +NED+E  A N++   +W + N+ PY  DVNI +ITVGN
Sbjct: 71  PNKDVLNALRGNRDIGVTVGIKNEDLEALAANKDAVKSWFSTNIDPYIADVNITFITVGN 130

Query: 123 EVVPGDTASPFVANAIKNMMQALDNAGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIAAR 182
           + +PGD   P V   I+++   + +  +Q  I ++T V  T L    PPSAG  +  A +
Sbjct: 131 QAIPGDKHGPHVLPVIQSLTDLVKSRNLQ--ISISTTVTTTSLARLKPPSAGVLTPQARQ 190

Query: 183 AMKDIGNVLESSCAPILVNVYPYFAYASNPQQISLSYALFTSTSPVVVDGDLQYFNLLDA 242
            +  +  +L  +  PI VN+YPY+ +AS+P+ + L YA F +   VV DG L+Y NL DA
Sbjct: 191 QLVPVLRLLSQTSTPIFVNIYPYYFHASDPKNVPLEYANFNNDQIVVKDGALKYSNLFDA 250

Query: 243 MVDSFYAALEKIGVEGVRIGISETGWPTKGNEPFTSVENALTYNKNFVEHVTSGVGTPRM 302
           + D+F  A+EK GV+G+ + +SETGWP+ GN   T+     TY  NFV+HV SG GTP+ 
Sbjct: 251 IFDAFLWAMEKEGVKGLPLVVSETGWPSAGNGGMTTPALQYTYIGNFVKHVASGKGTPKR 310

Query: 303 PNLQYDVVLFEMFNEDLKSPGVEQNFGFFDP 334
           PN + D  +FE +NE+ K  G+ Q+FG +DP
Sbjct: 311 PNSRIDAYIFETYNENQKPVGIYQHFGLYDP 336

The following BLAST results are available for this feature:

BLAST of Chy7G135200 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49352	3.8e-76	44.48	Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana OX=3702 GN...	[more]
O22317	6.5e-76	46.15	Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2	[more]
O49353	1.0e-73	44.83	Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q02439	4.4e-64	44.73	Putative glucan endo-1,3-beta-glucosidase GVI (Fragment) OS=Hordeum vulgare OX=4...	[more]
P23431	3.7e-63	43.67	Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform OS=Nicotiana plumbagini...	[more]

BLAST of Chy7G135200 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L1Z1	3.9e-193	98.26	(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_4G377700 PE=3...	[more]
A0A5A7V7B6	6.8e-177	90.12	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A5D3D504	6.8e-177	90.12	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S4DV56	3.9e-156	88.85	(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103487671 PE=3 SV...	[more]
A0A1S4DV56	1.9e-150	80.30	(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103487671 PE=3 SV...	[more]

BLAST of Chy7G135200 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031740439.1	1.93e-246	98.26	glucan endo-1,3-beta-glucosidase [Cucumis sativus]	[more]
KAE8649635.1	3.19e-231	98.20	hypothetical protein Csa_012736 [Cucumis sativus]	[more]
TYK18631.1	4.19e-225	90.12	putative glucan endo-1,3-beta-glucosidase BG4 [Cucumis melo var. makuwa]	[more]
KAA0064262.1	3.85e-220	90.12	putative glucan endo-1,3-beta-glucosidase BG4 [Cucumis melo var. makuwa]	[more]
TYK18632.1	1.28e-196	80.23	putative glucan endo-1,3-beta-glucosidase BG4 [Cucumis melo var. makuwa]	[more]

BLAST of Chy7G135200 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G77780.1	2.2e-79	47.77	Glycosyl hydrolase superfamily protein	[more]
AT5G20330.1	2.7e-77	44.48	beta-1,3-glucanase 4	[more]
AT5G20340.1	7.4e-75	44.83	beta-1,3-glucanase 5	[more]
AT1G77790.1	3.4e-72	47.44	Glycosyl hydrolase superfamily protein	[more]
AT5G20560.1	4.9e-71	40.79	Glycosyl hydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 29..342 e-value: 2.5E-113 score: 380.2
None	No IPR available	PANTHER	PTHR32227:SF248	GLYCOSYL HYDROLASE SUPERFAMILY PROTEIN	coord: 21..341
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 29..340 e-value: 5.1E-90 score: 302.2
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 21..341
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 29..341

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy7G135200.1	Chy7G135200.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds