Chy7G131980 (gene) Cucumber (hystrix) v1

Overview
NameChy7G131980
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionAlpha-amylase
LocationchrH07: 2971798 .. 2978301 (+)
RNA-Seq ExpressionChy7G131980
SyntenyChy7G131980
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGACCTTGCGACGCTTGTACCTTTGTTTCTTGGGCGCGGCTGAGTTGGAATTGACATCTTCGTCGTCGGATGTGGTGGTTGTAGATGACATTTTGAGCTTCTTCGGGTGGGGTTCTTCGATCAACGGTTGTGGCGGTGACCTTCTGGTTTCAGTTGGCGGTTCTTCTTCCATTATAGCCAGGTAGGGAAATGGACTGAAGTAGACATTAGGGTTTTAGAGACCATCCCATCCAACAGATTTTAGTAACTCCCAAACTCAATAATATCAATTTTTCTTTTTTCTTTTTAAATAATAATATTTGGGTTTTTTTTTTTTTTTTATTCACTTTTGAAAATAAATTTGTAATTTTCTAAATTTTTAAAAAGTAAATTGTCAAAAATAAAAAATAAAAATGGTCAAAACTAAAAATATTTGAAAATATTTAGTGCTACAGTAAATAGTTTGTCAATTTTTAATCCGTGAAAAAAACCTAAGTATGTATATTGTTAAAAATAGAAAAAATTAGTAAGTATATTTCATAGAAGAAAAACAGGTGTTGTAAATTTTGTGTTTGAGTTTTTTATGCAGTGTAAATAGTTTGTTTTTTCTTATAAAAAATCGATTTTAAAAATATCAATATTAATCCATGCAATTGACATTCTATTAGTTTGGTAGTCTATGTATAAGAATGAGTTTAGCTAGTTGTACCTAAAAACACATTACGTATCACCTAGTTATTAAAACTATGTTTTATTTTTTAACATTAGACCAACCATGATGGTTTAATACTATGCAACTCATAATTAAAAAATAGACCAAGTTAATTTTATGAAGTTTAGGGTTTCACGGAGCAATATCATTTTGGAGTAATTATCGACAAAATTTGAGAGTGAATGATTCGTAATCGCATTTTAAACTATAGTTTCATTTCTCTTTGATATTATGGGATGATTTATTAGTTAGTCAAATGGGTTATATGTTAAAATTATTATCGTTCGAGTCGGTTGAAACCAACTTATTTCACAATTCTCTTTATTTTATGTATCTCTTCGTAAATTACTCATTTTGAGCGTAAGATAACAAAAAATTAATCAAAATTTGTGAAAGTAAAGAGATGTGTATCTTGTAATTCCCTTTGATAGTAATCGAATAAACAATGAAACTCGAACCATTTCACTTAGTATTGCTTACTTCAAATCACTCCTCAAAGGTGTCCGACATAAGCATTATCTCTAACACAAATATATGAAATTTAATACATTGCATTATTGTGTTAAAAAGGTAATAGATAGACGTTTTTTTAAAATAAAATTACGAACAAAATAGAATCATATACAAAATTTATATATATATATATAAAAAGATTTAAACCAATAAATAAAGGGCAATATCGTGATTCAATTAGCATACGTTCATTTGGACGTTCCAGTGTTAGTGTTCCATAAAACTGACTGTTGCTTAACGCTTAAGCCATTGCAAAATGGTCAGAGACGGAACTCCACATCCCGCACCCCTTCGATTGGTTCTAGCCAAGAATCTCGAGCCCACGCGGCTGCAGTTCAATTGCCTCATTGCAAATGGTTCGAACAGACAAAATCGTTTCCCTGAACCAGCAGAACCAAGAACGTCGAACCGCGAAGTGATTGCTTCTTGGTAACGTTTTTCATTATTTGCTAAGTAACTTTTATTCACGATCGCCTTATGTTCTTGCGATGTTCAAATGCGGAATTCTAATCACATTACTTAGTGAAGCGTTTTGGTTGTTTAGCTATTTTCCCATTAAATATGTATACACGACGAATCATTCTCAAAGCATCTTTCGTTGTAATTGCGTTATTCTTTTACTCCTTCTGAATTGAGGATTCTTGCTAGCTGATCTTATCTCTTTCTTGAATTTTTCAGCGGTAAATGTCTTCGCGCCAATATGAAACTGTGATTTCAACTGATTAATTCGTATTGTCAAGGTTGACGCATCTGCTGCATAGCTATTTAGAAGTTGATTTTAGACAATTTTAAGAAGTGTTTATGGGTTATCCAGTTTCAAATGGAGGAATCGATTCTCAAAATGAATTGTCCACACCACGTCACTAAGAATTCTGTTATAATTCTTGCTCGTTTTCTTATTTTCTTTTTCGTTGTTGGAGTGGCCATCTGGATAATGAAGTTAAGACCACACAAGATGATTGAGGTTACACTTTGCAGATTGTGATGCTTTTTTATTTTCGTTGTAGGACTAGGACGTCCCTATATTCTGCAACCAAGACGGTTTTCACAAGTTTCTCCCAAGTATTATGGGTGACCATGATGATGTATGCTTCTTGTTTAATTTGGCAATTGTTTCCTTGATGAGTTTTCTGCATCAGTGTGATATGCTTTTAGTTATAAATTTGTTGGAAATTAGAATATACACTGTGATATACAAATTACTTATTTATGTTGGAATTCAGGTTTTTGATGAAAGCTACGAACAGACTGATAGTGGTATGGTTCTTTCTTTGTTTTGACATTAAATTTCACATCATGGTATGCTCACGTCAATCTAGTGTTTGTGAAAAGATCATACCTCTCTTGGAAGTGACTTCAAATTCTAATACATTTGTTTGGGTCTAACTAACCAAAGAGAATGCTTGATGATGCTTAAAATTTGACTCAAGGATAAGAAATTTGAATCGAAATATATGCTTCTTAGGTGTGAGTACGTTGCAATATTTGCTTTTAATTTACATAAAATTTGTGGTTTATGAAAAGGATTGATACACGTGGCCTTGAAATCAGAGCTGGTTTTTTGCAATGTAAATTTTCTTTTATTTGAAATTATCTTATCATTTTAATAATTTCAGTGTTTCTTTATGGTCACCTGTAGGGGCAGTGATACGCAATGGAAGAGAAATCCTTTTCCAGGTAGATGTTACAATTTCAGCTTTCTGAGTTTTCTCGAGAGCAGATGTCAGCAGGAAGGAAAATATTATGTTGCTCTTAATTGAATTGAAGTCTCGGAAACAAAAAAAAAGAAAAATGTTTGCCTTTTGAAATTCATGCTCGATGTTGTTGATTCTTTTAATGTAAATTGGTTAGATCAAATCTCCAGGTCATGTTAAACTTATGAGAGAAATTTTGCACCTATAATTTCCATTGTAATCACAGGCATTCAGCTGGGAATCTCATAAATATGATTGGTGGGAAAATCTAGAGAGTAAAGTTCATGATATCGCAAAAAGTGGATTTACCTCTGCATGGTTGCCTCCTCCAACTCATTCCATTGCTCCTCAAGGTCAAAGGAACAAATTCATTTGAATTTTTATGTTCCTTTCACGTATATGCGTGCATAACTCTTGATATTTTCTTTACAATTTCAGGTTACCTCCCTCAGAACCTTTACTCCCTAAACTCTTCATATGGTTCTGAGGACATGCTGAAAGCTTTACTTCTCAAAATGAAGCAGTGCAAGGTTAGAGCAATTGCTGACATAGTCATCAATCATCGTGTTGGGACTGTTGTAGGACATGGAGGAATGCATAATCGCTTTGATGGAATACCATTACCATGGAATGAGTATGCTGTTACCTCGTGTTCTGGTGGCCTGGTATTTTTCTGTTACAAATTCCAAGTGTTCTCATAATTATAAGAAAACCTTTGGTCTTTAGTCTGTGGTATCTGTTCTAGGGCAATCGTAGCACTGGTGACAACTTCTATGGGGTTCCAAACATTGATCATACCCAACCTTTTGTCCGTAGAGATATCATAGCATGGCTGAAGTGGCTACGCAGTAGTGTTGGATTTCAGGATTTTCGTTTTGATTTTGCAAGGGGGTAATATTGTCCTTCTCTGTTTGTGTGTGTGTGTATTTATATTGTGGGGAGGCTGGGGCGTTACAGAGGTTCAAGGATAAATTGTTCTGAAGATGTCAAGATCCCTCCTCTGTTTCAAAATTTCACTGTATCATCTTCTCAACATTCGGCTTGTCTTTTCTTTTAAGGAGTTCTTCAATGGATTATAACATACATTTGCCGTTTGCTTTTATTTTTGATGTTTTAACTCAGTTTTTCTGCAAAATTCGTGAAAGAATATATTGAAGCGGCAAAGCCAATGTTTTCTATTGGGGAATATTGGGATTCTTGTAATTATAATGGCCATGACTTGGACTATAATCAAGGTAACATGATGATGCATTATGTTGATAAATTGAAATCATGTTTTCAAGTTTCTGGATTTTTAGTTTTTCTTAATGGTTTTGTCCGTGAATAATATCCTATGTTCGTCCTATCAATTCATGTGTTCTATATTGATGATTACAACAATAAATTCAGATGGACATAGACAGCGGATAATAAATTGGATTGATGGCACAGGACAACTTTCAGCTGCTTTTGATTTTACAACTAAGGGAGTCCTTCAGGTATTGGTGTTGTGTGAGGCCATTGCTAACTCCCTTCCCTCCTCAAAATTGTAATACTACGGACCTCAAATCTTAATTACTTAAAATAGAAAGGATGAAGTGACAGCTATGTCATGGTTTTTCTTTCCCTCTAGATCAGTAGATCTACAATGTTTGACTTTTCTTCACAACTCTTTCTATTTGCTTTTTTTCTCCTTCTTTATTGAGTGATGTTTGGGTTTCGTTAATATTTGCTCTTGCTAGTACATTGCTTGACTAACATGCATTTGTTTCGTTGTTTGCTTCGTAATAAACATAATATAATTAATTTTGCTTGTAGGAAGCTGTTAGAAGACAATTATGGCGTCTGCGTGACTGTCAAGGAAAGCCACCAGGTGTGATGGGATGGTGGCCTTCAAGGGCCGTGACATTCTTAGATAACCATGATACCGGTTCAACGCAGGTACCACTTCTTCAAATCTGTTTGTGAAATTATTGGTTGACAAATTTCAACATTTGTTTTCATTGCTCGTTGTGCATGTGCGTTATAAAATTGTCTCAACTTGACCACGTATCCATGTCCTTTTATTTGGATTAATATTTGAGAACACGTGTTTGGAAGTCATGCCTTGTATTGAGGATTGAGTGTGATCAAACCATACATGAAAATGTCATAGGAAGCAAGGAGAAAAGTAGTATTGATGCATACCTGAATGAAGTTGTAATGTCTGGTTGTTCTACACAGCTTCTATATGACCAAATATGGTGCCATTCAGAACAGCTACTGGACGCTGTTCCATTTGCCTGTGTTGCTATTATCTTTTGTAAGATGCGTATGTGTTTTTTGCAACATGTAACTGAGATTTATTAGCCAAGCTTTCTTTTATATAGGTTTGTTCAAATTGAACTAATTCAAATGTAAATAGTTCCAGGCTTCTAGCAATCCAACTCTGCATCTTAATAAGTAGATAGCTTTAGATTATTATTTGGAAACTCTTAATTAATTTTTTTCCCTAGGTTGAAATCAAACATCAAGTGTGCTTTCAAGTATAGATATTGACTTAAGCAGATCATGACAGTTAATGCAACTCGACATGCTTGGCAAATGAACTATATAATTCTTCATATGTATTTTCTTGAAACATTTATAGAAGCTGAAATTGTACCATTAGAATGTTGGTGACTCCACTAAACTCAGCATACCATTGTTCTTGCAGGCCCATTGGCCCTTTCCTGCCAACCATATCATGGAGGTATACAAGTGTGGCATATCATTCCATTTGCAATCTCTTTATTGCTTATAGTCTTATTCTTGCTGTGTTGTGTCTTCTTCGTGGTTTTTTGAAGCTCTCAATTGTTTGGTCCAATTACTCGTTGTGTAAAATCTAATTACCCCCCATCAGGTGGTCCTCATTGTTCTTTGTGTGTTTTGGAAGCTTTCAATTGTTTGTTTCAATTGATCCTATGTACTACTCTGTCTACATTTGAAGTTAGTTTCCTACTTTTAGCTGCAAATTCTATGGCTATAACTGTTCAGTATGCATTTAAAAAAAAAAAAACTGTTCAGCATACTAAGCGGTCAATCTTTGATGTTTCTGGGTGAATTCCATTGAAAGAACTTCATAATTATTTTCTAGTCTTACAATTGTTACCGCAACTACTTTTATGCTTGGCCTAGAGTCTTCACTTGTCTTCCAGTAAAAAAAAAAATTCCATCATGCATCTATTTTCTGATTCCTGGAGCTTTGGTTATTCCCTGCAGGGCTATGCATATATTCTCACGCACCCAGGGATACCAACAGTATTTTATGACCACTTATACGAATGGGGTGACTCAGTGCACGACGAGATAGTAAAATTAGTATGTTGCATAGTCAATAATGCTTCGATAGAGTTTGTTATCTTCTATTCAAGCAAACTTCTCATAGTACTTAAATTTTATTTTTCTTGCAGATAGACATTCGAAAACATCAAGACATCAACAGTCGGTCATCTGTGAAGATCCTTGAGGCACGATCAGATCTTTATTCTGCAATAATTGGGGAGAAATTGTGCATGAAGATCGGAGATGGTTCTTGGTGTCCTTCTGGTACGGAATGGACTCTTGCAACCTCTGGCTGGAGATATGCAGTGTGGCAGAAATAA

mRNA sequence

ATGGCGACCTTGCGACGCTTGTACCTTTGTTTCTTGGGCGCGGCTGAGTTGGAATTGACATCTTCGTCGTCGGATGTGGTGGTTGTAGATGACATTTTGAGCTTCTTCGGGTGGGGTTCTTCGATCAACGGTTGTGGCGGTGACCTTCTGGTTTCAGTTGGCGGTTCTTCTTCCATTATAGCCAGAGACGGAACTCCACATCCCGCACCCCTTCGATTGGTTCTAGCCAAGAATCTCGAGCCCACGCGGCTGCAGTTCAATTGCCTCATTGCAAATGGTTCGAACAGACAAAATCGTTTCCCTGAACCAGCAGAACCAAGAACGTCGAACCGCGAAGTTTTTGATGAAAGCTACGAACAGACTGATAGTGGGGCAGTGATACGCAATGGAAGAGAAATCCTTTTCCAGGCATTCAGCTGGGAATCTCATAAATATGATTGGTGGGAAAATCTAGAGAGTAAAGTTCATGATATCGCAAAAAGTGGATTTACCTCTGCATGGTTGCCTCCTCCAACTCATTCCATTGCTCCTCAAGGTTACCTCCCTCAGAACCTTTACTCCCTAAACTCTTCATATGGTTCTGAGGACATGCTGAAAGCTTTACTTCTCAAAATGAAGCAGTGCAAGGTTAGAGCAATTGCTGACATAGTCATCAATCATCGTGTTGGGACTGTTGTAGGACATGGAGGAATGCATAATCGCTTTGATGGAATACCATTACCATGGAATGAGTATGCTGTTACCTCGTGTTCTGGTGGCCTGGGCAATCGTAGCACTGGTGACAACTTCTATGGGGTTCCAAACATTGATCATACCCAACCTTTTGTCCGTAGAGATATCATAGCATGGCTGAAGTGGCTACGCAGTAGTGTTGGATTTCAGGATTTTCGTTTTGATTTTGCAAGGGGTTTTTCTGCAAAATTCGTGAAAGAATATATTGAAGCGGCAAAGCCAATGTTTTCTATTGGGGAATATTGGGATTCTTGTAATTATAATGGCCATGACTTGGACTATAATCAAGATGGACATAGACAGCGGATAATAAATTGGATTGATGGCACAGGACAACTTTCAGCTGCTTTTGATTTTACAACTAAGGGAGTCCTTCAGGAAGCTGTTAGAAGACAATTATGGCGTCTGCGTGACTGTCAAGGAAAGCCACCAGGTGTGATGGGATGGTGGCCTTCAAGGGCCGTGACATTCTTAGATAACCATGATACCGGTTCAACGCAGGCCCATTGGCCCTTTCCTGCCAACCATATCATGGAGGGCTATGCATATATTCTCACGCACCCAGGGATACCAACAGTATTTTATGACCACTTATACGAATGGGGTGACTCAGTGCACGACGAGATAGTAAAATTAATAGACATTCGAAAACATCAAGACATCAACAGTCGGTCATCTGTGAAGATCCTTGAGGCACGATCAGATCTTTATTCTGCAATAATTGGGGAGAAATTGTGCATGAAGATCGGAGATGGTTCTTGGTGTCCTTCTGGTACGGAATGGACTCTTGCAACCTCTGGCTGGAGATATGCAGTGTGGCAGAAATAA

Coding sequence (CDS)

ATGGCGACCTTGCGACGCTTGTACCTTTGTTTCTTGGGCGCGGCTGAGTTGGAATTGACATCTTCGTCGTCGGATGTGGTGGTTGTAGATGACATTTTGAGCTTCTTCGGGTGGGGTTCTTCGATCAACGGTTGTGGCGGTGACCTTCTGGTTTCAGTTGGCGGTTCTTCTTCCATTATAGCCAGAGACGGAACTCCACATCCCGCACCCCTTCGATTGGTTCTAGCCAAGAATCTCGAGCCCACGCGGCTGCAGTTCAATTGCCTCATTGCAAATGGTTCGAACAGACAAAATCGTTTCCCTGAACCAGCAGAACCAAGAACGTCGAACCGCGAAGTTTTTGATGAAAGCTACGAACAGACTGATAGTGGGGCAGTGATACGCAATGGAAGAGAAATCCTTTTCCAGGCATTCAGCTGGGAATCTCATAAATATGATTGGTGGGAAAATCTAGAGAGTAAAGTTCATGATATCGCAAAAAGTGGATTTACCTCTGCATGGTTGCCTCCTCCAACTCATTCCATTGCTCCTCAAGGTTACCTCCCTCAGAACCTTTACTCCCTAAACTCTTCATATGGTTCTGAGGACATGCTGAAAGCTTTACTTCTCAAAATGAAGCAGTGCAAGGTTAGAGCAATTGCTGACATAGTCATCAATCATCGTGTTGGGACTGTTGTAGGACATGGAGGAATGCATAATCGCTTTGATGGAATACCATTACCATGGAATGAGTATGCTGTTACCTCGTGTTCTGGTGGCCTGGGCAATCGTAGCACTGGTGACAACTTCTATGGGGTTCCAAACATTGATCATACCCAACCTTTTGTCCGTAGAGATATCATAGCATGGCTGAAGTGGCTACGCAGTAGTGTTGGATTTCAGGATTTTCGTTTTGATTTTGCAAGGGGTTTTTCTGCAAAATTCGTGAAAGAATATATTGAAGCGGCAAAGCCAATGTTTTCTATTGGGGAATATTGGGATTCTTGTAATTATAATGGCCATGACTTGGACTATAATCAAGATGGACATAGACAGCGGATAATAAATTGGATTGATGGCACAGGACAACTTTCAGCTGCTTTTGATTTTACAACTAAGGGAGTCCTTCAGGAAGCTGTTAGAAGACAATTATGGCGTCTGCGTGACTGTCAAGGAAAGCCACCAGGTGTGATGGGATGGTGGCCTTCAAGGGCCGTGACATTCTTAGATAACCATGATACCGGTTCAACGCAGGCCCATTGGCCCTTTCCTGCCAACCATATCATGGAGGGCTATGCATATATTCTCACGCACCCAGGGATACCAACAGTATTTTATGACCACTTATACGAATGGGGTGACTCAGTGCACGACGAGATAGTAAAATTAATAGACATTCGAAAACATCAAGACATCAACAGTCGGTCATCTGTGAAGATCCTTGAGGCACGATCAGATCTTTATTCTGCAATAATTGGGGAGAAATTGTGCATGAAGATCGGAGATGGTTCTTGGTGTCCTTCTGGTACGGAATGGACTCTTGCAACCTCTGGCTGGAGATATGCAGTGTGGCAGAAATAA

Protein sequence

MATLRRLYLCFLGAAELELTSSSSDVVVVDDILSFFGWGSSINGCGGDLLVSVGGSSSIIARDGTPHPAPLRLVLAKNLEPTRLQFNCLIANGSNRQNRFPEPAEPRTSNREVFDESYEQTDSGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAIADIVINHRVGTVVGHGGMHNRFDGIPLPWNEYAVTSCSGGLGNRSTGDNFYGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQRIINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVKLIDIRKHQDINSRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK*
Homology
BLAST of Chy7G131980 vs. ExPASy Swiss-Prot
Match: Q8LFG1 (Probable alpha-amylase 2 OS=Arabidopsis thaliana OX=3702 GN=AMY2 PE=2 SV=1)

HSP 1 Score: 726.1 bits (1873), Expect = 2.8e-208
Identity = 320/407 (78.62%), Postives = 371/407 (91.15%), Query Frame = 0

Query: 113 VFDESYEQTDSGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSAWLPPPT 172
           VFDE  +QTD G VIR+GRE++ QA++WESHKYDWW NL+ KV DIAKSGFTSAWLPPP+
Sbjct: 7   VFDECNDQTDIGRVIRDGREVILQAYNWESHKYDWWRNLDGKVPDIAKSGFTSAWLPPPS 66

Query: 173 HSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAIADIVINHRVGTVVGHGGMH 232
            S+AP+GYLPQ+LYSLNS+YGSE +LK+LL KMKQ KVRA+ADIVINHRVGT  GHGGM+
Sbjct: 67  QSLAPEGYLPQDLYSLNSAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTRGHGGMY 126

Query: 233 NRFDGIPLPWNEYAVTSCSGGLGNRSTGDNFYGVPNIDHTQPFVRRDIIAWLKWLRSSVG 292
           NR+DGI LPW+E+AVTSC+GGLGNRSTGDNF GVPN+DHTQ FVR+DII WL+WLR++VG
Sbjct: 127 NRYDGISLPWDEHAVTSCTGGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRWLRNTVG 186

Query: 293 FQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQRIINWID 352
           FQDFRFDFARG+SA +VKEYI AAKP+FS+GE WDSCNYNGH LDYNQD HRQRII+WID
Sbjct: 187 FQDFRFDFARGYSANYVKEYIGAAKPLFSVGECWDSCNYNGHGLDYNQDSHRQRIISWID 246

Query: 353 GTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQA 412
            TGQ+SAAFDFTTKG+LQEAV+ Q WRL D QGKPPGVMGWWPSRAVTFLDNHDTGSTQA
Sbjct: 247 ATGQISAAFDFTTKGILQEAVKGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHDTGSTQA 306

Query: 413 HWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVKLIDIRKHQDINSRSSVK 472
           HWPFP++H+MEGYAYILTHPGIP VFYDH Y+WG S+HD+IVKLIDIR+ QDI+SRS+V+
Sbjct: 307 HWPFPSHHVMEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVR 366

Query: 473 ILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 520
           +L+A S+LY+AI+GEK+CMK+GDGSWCPSG +WTLATSG RYAVW K
Sbjct: 367 VLKAESNLYAAIVGEKICMKLGDGSWCPSGRDWTLATSGHRYAVWHK 413

BLAST of Chy7G131980 vs. ExPASy Swiss-Prot
Match: Q94A41 (Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AMY3 PE=1 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 2.5e-116
Identity = 196/397 (49.37%), Postives = 272/397 (68.51%), Query Frame = 0

Query: 129 NGREILFQAFSWESHKYD-WWENLESKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYS 188
           +G EIL Q F+WES+K   W+  L+ K  ++A  GFT  WLPPPT S++P+GY+P++LY+
Sbjct: 493 SGFEILCQGFNWESNKSGRWYLELQEKADELASLGFTVLWLPPPTESVSPEGYMPKDLYN 552

Query: 189 LNSSYGSEDMLKALLLKMKQCKVRAIADIVINHRVGTVVGHGGMHNRFDGIPLPWNEYAV 248
           LNS YG+ D LK  + K  +  ++ + D V+NHR        G+ N F G  L W++ AV
Sbjct: 553 LNSRYGTIDELKDTVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGG-RLNWDDRAV 612

Query: 249 TSCS---GGLGNRSTGDNFYGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGF 308
            +      G GN+S+GDNF+  PNIDH+Q FVR+DI  WL W+   VG+  +R DF RGF
Sbjct: 613 VADDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWMMEEVGYDGWRLDFVRGF 672

Query: 309 SAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQRIINWIDGTGQLSAAFDFT 368
              +VK+Y++A+KP F++GEYWDS +Y   ++DYNQD HRQRI++WI+ T   + AFD T
Sbjct: 673 WGGYVKDYMDASKPYFAVGEYWDSLSYTYGEMDYNQDAHRQRIVDWINATSGAAGAFDVT 732

Query: 369 TKGVLQEAVRR-QLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIME 428
           TKG+L  A+++ + WRL D +GKPPGV+GWWPSRAVTF++NHDTGSTQ HW FP    M+
Sbjct: 733 TKGILHTALQKCEYWRLSDPKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPEGKEMQ 792

Query: 429 GYAYILTHPGIPTVFYDHLYEWGDSVHDEIVKLIDIRKHQDINSRSSVKILEARSDLYSA 488
           GYAYILTHPG P VF+DH++      H EI  L+ +R  Q ++ RS V I ++  D+Y+A
Sbjct: 793 GYAYILTHPGTPAVFFDHIF---SDYHSEIAALLSLRNRQKLHCRSEVNIDKSERDVYAA 852

Query: 489 IIGEKLCMKIGDGSW-CPSGTE-WTLATSGWRYAVWQ 519
           II EK+ MKIG G +  P+G++ W++A  G  Y VW+
Sbjct: 853 IIDEKVAMKIGPGHYEPPNGSQNWSVAVEGRDYKVWE 885

BLAST of Chy7G131980 vs. ExPASy Swiss-Prot
Match: P17859 (Alpha-amylase OS=Vigna mungo OX=3915 GN=AMY1.1 PE=2 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 4.2e-103
Identity = 183/402 (45.52%), Postives = 261/402 (64.93%), Query Frame = 0

Query: 133 ILFQAFSWESHKY-DWWENLESKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLNSS 192
           +LFQ F+WES K   W+ +L++ + D+A +G T  WLPPP+ S++P+GYLP  LY L++S
Sbjct: 25  LLFQGFNWESSKKGGWYNSLKNSIPDLANAGITHVWLPPPSQSVSPEGYLPGRLYDLDAS 84

Query: 193 -YGSEDMLKALLLKMKQCKVRAIADIVINHRVGTVVGHGGMHNRFDG----IPLPWNEYA 252
            YGS++ LK+L+    +  ++ +ADIVINHR        G++  F+G        W    
Sbjct: 85  KYGSKNELKSLIAAFHEKGIKCLADIVINHRTAERKDGRGIYCIFEGGTPDSRQDWGPSF 144

Query: 253 V----TSCSGGLGNRSTGDNFYGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFAR 312
           +    T+ S G GN  +G+ +   P+IDH  P V+R++  W+ WL++ +GF  +RFDF +
Sbjct: 145 ICRDDTAYSDGTGNNDSGEGYDAAPDIDHLNPQVQRELSEWMNWLKTEIGFDGWRFDFVK 204

Query: 313 GFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHD--LDYNQDGHRQRIINWIDGTGQLSAA 372
           G++    K Y+E  KP F++GE WDS +Y G D   +YNQD HR  ++NW++  G    A
Sbjct: 205 GYAPSISKIYMEQTKPDFAVGEKWDSISY-GQDGKPNYNQDSHRGALVNWVESAGGAITA 264

Query: 373 FDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANH 432
           FDFTTKG+LQ AV+ +LWRL D  GKPPG++G  P  AVTF+DNHDTGSTQ  WPFP++ 
Sbjct: 265 FDFTTKGILQAAVQGELWRLIDPNGKPPGMIGVKPENAVTFIDNHDTGSTQRLWPFPSDK 324

Query: 433 IMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVKLIDIRKHQDINSRSSVKILEARSDL 492
           +M+GYAYILTHPG P++FYDH ++WG  + ++I KL  IR    IN +S+VKI+ +  DL
Sbjct: 325 VMQGYAYILTHPGTPSIFYDHFFDWG--LKEQIAKLSSIRLRNGINEKSTVKIMASEGDL 384

Query: 493 YSAIIGEKLCMKIGD----GSWCPSGTEWTLATSGWRYAVWQ 519
           Y A I  K+ +KIG     G+  PS     +ATSG  YAVW+
Sbjct: 385 YVAKIDNKIMVKIGPKMDLGNLIPSNLH--VATSGQDYAVWE 421

BLAST of Chy7G131980 vs. ExPASy Swiss-Prot
Match: P27932 (Alpha-amylase isozyme 3A OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.2 PE=2 SV=2)

HSP 1 Score: 375.9 bits (964), Expect = 7.2e-103
Identity = 182/404 (45.05%), Postives = 260/404 (64.36%), Query Frame = 0

Query: 132 EILFQAFSWESHKYD--WWENLESKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLN 191
           +ILFQ F+W+S K    W+  L+ +V DIA +G T  WLPPPTHS++PQGY+P  LY LN
Sbjct: 29  QILFQGFNWDSWKKQGGWYNMLKDQVGDIASAGVTHVWLPPPTHSVSPQGYMPGRLYDLN 88

Query: 192 SS-YGSEDMLKALLLKMKQCKVRAIADIVINHRVGTVVGHGGMHNRFDGIP----LPWNE 251
           +S YG++  LK+L+       ++ +ADIV+NHR        G++  F G      L W  
Sbjct: 89  ASKYGTKAELKSLIAAFHAKGIKCVADIVVNHRCADDKDGRGVYCIFKGGGPRGCLDWGP 148

Query: 252 YAV----TSCSGGLGNRSTGDNFYGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDF 311
             +    T  S G G+R TG +F   P+IDH  P V+R++  WL+WLR  VGF  +R DF
Sbjct: 149 SMICCDDTQYSDGTGHRDTGADFAAAPDIDHLNPLVQRELSDWLRWLRRDVGFDGWRLDF 208

Query: 312 ARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLD-YNQDGHRQRIINWIDGTGQLSA 371
           A+G+SA   + Y++ A+P F + E W+S +Y+G      NQDG RQ ++NW+   G  + 
Sbjct: 209 AKGYSAAVARTYVQNARPSFVVAEIWNSLSYDGDGKPAANQDGQRQELVNWVKQVGGPAT 268

Query: 372 AFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPAN 431
           AFDFTTKG+LQ AV+ +LWR+RD  GK PG++GW+P +AVTF+DNHDTGSTQ  WPFP++
Sbjct: 269 AFDFTTKGILQSAVQGELWRMRDKDGKAPGMIGWYPEKAVTFVDNHDTGSTQRMWPFPSD 328

Query: 432 HIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVKLIDIRKHQDINSRSSVKILEARSD 491
            ++ GYAYILTHPG+P +FYDH+++W  ++  EI  L   RK   IN+ S +++L A SD
Sbjct: 329 KVILGYAYILTHPGVPCIFYDHVFDW--NLKQEINALAATRKRNGINAGSKLRVLAAESD 388

Query: 492 LYSAIIGEKLCMKIGD----GSWCPSGTEWTLATSGWRYAVWQK 520
           +Y A++ E++  KIG     G+  PS  ++ +   G  Y VW+K
Sbjct: 389 MYVAMVDERVITKIGPRIDVGNIIPS--DFHIVAHGNDYCVWEK 428

BLAST of Chy7G131980 vs. ExPASy Swiss-Prot
Match: P27934 (Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.4 PE=2 SV=1)

HSP 1 Score: 375.9 bits (964), Expect = 7.2e-103
Identity = 185/404 (45.79%), Postives = 257/404 (63.61%), Query Frame = 0

Query: 132 EILFQAFSWESHKYD--WWENLESKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLN 191
           ++LFQ F+WES +    W+  L  KV +IA +G T  WLPPP+HS++PQGY+P  LY L+
Sbjct: 26  QVLFQGFNWESWRKQGGWYNFLHEKVEEIASTGATHVWLPPPSHSVSPQGYMPGRLYDLD 85

Query: 192 SS-YGSEDMLKALLLKMKQCKVRAIADIVINHRVGTVVGHGGMHNRFD-GIP---LPWNE 251
           +S YG+E  LK+L+       V  +ADIVINHR        G++  F+ G P   L W  
Sbjct: 86  ASKYGTEAELKSLIEAFHDKNVECLADIVINHRCADYKDSRGVYCVFEGGTPDGRLDWGP 145

Query: 252 YAV----TSCSGGLGNRSTGDNFYGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDF 311
             +    T  S G G+R TG  F   P+IDH  P V+R++  WL WLR+ +GF  +R DF
Sbjct: 146 DMICSDDTQYSNGRGHRDTGAGFGAAPDIDHLNPRVQRELTDWLNWLRTDLGFDGWRLDF 205

Query: 312 ARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDL-DYNQDGHRQRIINWIDGTGQLSA 371
           A+G+SA   + Y++   P F +GE W S  YNG      NQD  RQ ++NW++G G+ + 
Sbjct: 206 AKGYSAPLARIYVDNTNPTFVVGEIWSSLIYNGDGKPSTNQDADRQELVNWVEGVGKPAT 265

Query: 372 AFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPAN 431
           AFDFTTKG+LQ AV+ +LWRL D  GK PG+MGW P +AVTF+DNHDTGSTQ+ WPFP++
Sbjct: 266 AFDFTTKGILQAAVQGELWRLHDGNGKAPGLMGWMPDQAVTFVDNHDTGSTQSLWPFPSD 325

Query: 432 HIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVKLIDIRKHQDINSRSSVKILEARSD 491
            +M+GYAYILTHPGIP +FYDH+++W  ++  EI  L +IR    I++ S++ IL+A  D
Sbjct: 326 KVMQGYAYILTHPGIPCIFYDHVFDW--NLQHEIATLAEIRSRNGIHAESTLDILKAEGD 385

Query: 492 LYSAIIGEKLCMKIGD----GSWCPSGTEWTLATSGWRYAVWQK 520
           +Y A+I  K+  K+G     G   PS  ++ +   G  Y VW+K
Sbjct: 386 IYVAMIDGKVITKLGPRYDAGGIIPS--DFHVVAHGNDYCVWEK 425

BLAST of Chy7G131980 vs. ExPASy TrEMBL
Match: A0A5A7VG02 (Alpha-amylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G003440 PE=3 SV=1)

HSP 1 Score: 870.9 bits (2249), Expect = 2.6e-249
Identity = 416/470 (88.51%), Postives = 429/470 (91.28%), Query Frame = 0

Query: 62  RDGTPHPAPLRLVLAKNLEPTRLQFNCLIANGSNRQNRFPEPAEPRT------------S 121
           RDG+ HP PLRL LAKNLEPTRLQ NCLIANG  R    P   +PR              
Sbjct: 3   RDGSLHPTPLRLALAKNLEPTRLQINCLIANGLGR----PYLLQPRRQGFHKFLPSIMGD 62

Query: 122 NREVFDESYEQTDSGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSAWLP 181
           +  VFDE+YEQTD+GAVIRNGREILFQAF+WESHK DW  NLE KVHDIAKSGFTSAWLP
Sbjct: 63  HDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSAWLP 122

Query: 182 PPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAIADIVINHRVGTVVGHG 241
           PPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRA+ADIVINHRVGTVVGHG
Sbjct: 123 PPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHG 182

Query: 242 GMHNRFDGIPLPWNEYAVTSCSGGLGNRSTGDNFYGVPNIDHTQPFVRRDIIAWLKWLRS 301
           GMHNRFDGI LPW+E+AVTSCSGGLGNRSTGDNF+GVPNIDHTQPFVRRDIIAWLKWLRS
Sbjct: 183 GMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRS 242

Query: 302 SVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQRIIN 361
           SVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQRIIN
Sbjct: 243 SVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQRIIN 302

Query: 362 WIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGS 421
           WIDGTGQLSAAFDFTTKGVLQEAV+ QLWRL DC+GKPPGVMGWWPSRAVTFLDNHDTGS
Sbjct: 303 WIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHDTGS 362

Query: 422 TQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVKLIDIRKHQDINSRS 481
           TQAHWPFPANHIMEGYAYILTHPGIPTVFYDH Y+WGDSVHDEIVKLIDIRKHQDINSRS
Sbjct: 363 TQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDINSRS 422

Query: 482 SVKILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 520
           SVKILEARSDLYSAIIGE LCMKIGDGSWCP GTEWTLAT G RYAVWQK
Sbjct: 423 SVKILEARSDLYSAIIGENLCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 467

BLAST of Chy7G131980 vs. ExPASy TrEMBL
Match: A0A5D3D8R2 (Alpha-amylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00850 PE=3 SV=1)

HSP 1 Score: 865.1 bits (2234), Expect = 1.4e-247
Identity = 416/474 (87.76%), Postives = 429/474 (90.51%), Query Frame = 0

Query: 62  RDGTPHPAPLRLVLAKNLEPTRLQFNCLIANGSNRQNRFPEPAEPRT------------S 121
           RDG+ HP PLRL LAKNLEPTRLQ NCLIANG  R    P   +PR              
Sbjct: 3   RDGSLHPTPLRLALAKNLEPTRLQINCLIANGLGR----PYLLQPRRQGFHKFLPSIMGD 62

Query: 122 NREVFDESYEQTDSGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSAWLP 181
           +  VFDE+YEQTD+GAVIRNGREILFQAF+WESHK DW  NLE KVHDIAKSGFTSAWLP
Sbjct: 63  HDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSAWLP 122

Query: 182 PPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAIADIVINHRVGTVVGHG 241
           PPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRA+ADIVINHRVGTVVGHG
Sbjct: 123 PPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHG 182

Query: 242 GMHNRFDGIPLPWNEYAVTSCSGGLGNRSTGDNFYGVPNIDHTQPFVRRDIIAWLKWLRS 301
           GMHNRFDGI LPW+E+AVTSCSGGLGNRSTGDNF+GVPNIDHTQPFVRRDIIAWLKWLRS
Sbjct: 183 GMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRS 242

Query: 302 SVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQ----DGHRQ 361
           SVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQ    DGHRQ
Sbjct: 243 SVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQALNADGHRQ 302

Query: 362 RIINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNH 421
           RIINWIDGTGQLSAAFDFTTKGVLQEAV+ QLWRL DC+GKPPGVMGWWPSRAVTFLDNH
Sbjct: 303 RIINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNH 362

Query: 422 DTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVKLIDIRKHQDI 481
           DTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDH Y+WGDSVHDEIVKLIDIRKHQDI
Sbjct: 363 DTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDI 422

Query: 482 NSRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 520
           NSRSSVKILEARSDLYSAIIGE LCMKIGDGSWCP GTEWTLAT G RYAVWQK
Sbjct: 423 NSRSSVKILEARSDLYSAIIGENLCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 471

BLAST of Chy7G131980 vs. ExPASy TrEMBL
Match: A0A0A0L5R6 (Alpha-amylase OS=Cucumis sativus OX=3659 GN=Csa_4G652880 PE=3 SV=1)

HSP 1 Score: 855.1 bits (2208), Expect = 1.5e-244
Identity = 397/407 (97.54%), Postives = 400/407 (98.28%), Query Frame = 0

Query: 113 VFDESYEQTDSGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSAWLPPPT 172
           VFDESYE+TD+GAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSAWLPPPT
Sbjct: 7   VFDESYEKTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSAWLPPPT 66

Query: 173 HSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAIADIVINHRVGTVVGHGGMH 232
           HSIAPQGYLPQNLYSLNSSYGSEDMLK LLLKMKQCKVRAIADIVINHRVGTVVGHGGMH
Sbjct: 67  HSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVVGHGGMH 126

Query: 233 NRFDGIPLPWNEYAVTSCSGGLGNRSTGDNFYGVPNIDHTQPFVRRDIIAWLKWLRSSVG 292
           NRFDGI LPWNEYAVTSCSGGLGNRSTGDNF+GVPNIDHTQPFVRRDIIAWLKWLRSSVG
Sbjct: 127 NRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVG 186

Query: 293 FQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQRIINWID 352
           FQDFRFDFARGFSAKFVKEYIEA KPM SIGEYWDSCNYNGHDLDYNQDGHRQRIINWID
Sbjct: 187 FQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQRIINWID 246

Query: 353 GTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQA 412
           GTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQA
Sbjct: 247 GTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQA 306

Query: 413 HWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVKLIDIRKHQDINSRSSVK 472
           HWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEI   IDIRKHQDINSRSSVK
Sbjct: 307 HWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEI---IDIRKHQDINSRSSVK 366

Query: 473 ILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 520
           ILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK
Sbjct: 367 ILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 410

BLAST of Chy7G131980 vs. ExPASy TrEMBL
Match: A0A1S3BW59 (Alpha-amylase OS=Cucumis melo OX=3656 GN=LOC103493790 PE=3 SV=1)

HSP 1 Score: 825.1 bits (2130), Expect = 1.7e-235
Identity = 385/407 (94.59%), Postives = 395/407 (97.05%), Query Frame = 0

Query: 113 VFDESYEQTDSGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSAWLPPPT 172
           VFDE+YEQTD+GAVIRNGREILFQAF+WESHK DW  NLE KVHDIAKSGFTSAWLPPPT
Sbjct: 32  VFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSAWLPPPT 91

Query: 173 HSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAIADIVINHRVGTVVGHGGMH 232
           HSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRA+ADIVINHRVGTVVGHGGMH
Sbjct: 92  HSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHGGMH 151

Query: 233 NRFDGIPLPWNEYAVTSCSGGLGNRSTGDNFYGVPNIDHTQPFVRRDIIAWLKWLRSSVG 292
           NRFDGI LPW+E+AVTSCSGGLGNRSTGDNF+GVPNIDHTQPFVRRDIIAWLKWLRSSVG
Sbjct: 152 NRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVG 211

Query: 293 FQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQRIINWID 352
           FQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQRIINWID
Sbjct: 212 FQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQRIINWID 271

Query: 353 GTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQA 412
           GTGQLSAAFDFTTKGVLQEAV+ QLWRL DC+GKPPGVMGWWPSRAVTFLDNHDTGSTQA
Sbjct: 272 GTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHDTGSTQA 331

Query: 413 HWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVKLIDIRKHQDINSRSSVK 472
           HWPFPANHIMEGYAYILTHPGIPTVFYDH Y+WGDSVHDEIVKLIDIRKHQDINSRSSVK
Sbjct: 332 HWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDINSRSSVK 391

Query: 473 ILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 520
           ILEARSDLYSAIIGE LCMKIGDGSWCP GTEWTLAT G RYAVWQK
Sbjct: 392 ILEARSDLYSAIIGENLCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 437

BLAST of Chy7G131980 vs. ExPASy TrEMBL
Match: E5GCD3 (Alpha-amylase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 819.3 bits (2115), Expect = 9.1e-234
Identity = 385/411 (93.67%), Postives = 395/411 (96.11%), Query Frame = 0

Query: 113 VFDESYEQTDSGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSAWLPPPT 172
           VFDE+YEQTD+GAVIRNGREILFQAF+WESHK DW  NLE KVHDIAKSGFTSAWLPPPT
Sbjct: 7   VFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSAWLPPPT 66

Query: 173 HSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAIADIVINHRVGTVVGHGGMH 232
           HSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRA+ADIVINHRVGTVVGHGGMH
Sbjct: 67  HSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHGGMH 126

Query: 233 NRFDGIPLPWNEYAVTSCSGGLGNRSTGDNFYGVPNIDHTQPFVRRDIIAWLKWLRSSVG 292
           NRFDGI LPW+E+AVTSCSGGLGNRSTGDNF+GVPNIDHTQPFVRRDIIAWLKWLRSSVG
Sbjct: 127 NRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVG 186

Query: 293 FQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQ----DGHRQRII 352
           FQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQ    DGHRQRII
Sbjct: 187 FQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQALNADGHRQRII 246

Query: 353 NWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTG 412
           NWIDGTGQLSAAFDFTTKGVLQEAV+ QLWRL DC+GKPPGVMGWWPSRAVTFLDNHDTG
Sbjct: 247 NWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHDTG 306

Query: 413 STQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVKLIDIRKHQDINSR 472
           STQAHWPFPANHIMEGYAYILTHPGIPTVFYDH Y+WGDSVHDEIVKLIDIRKHQDINSR
Sbjct: 307 STQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDINSR 366

Query: 473 SSVKILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 520
           SSVKILEARSDLYSAIIGE LCMKIGDGSWCP GTEWTLAT G RYAVWQK
Sbjct: 367 SSVKILEARSDLYSAIIGENLCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 416

BLAST of Chy7G131980 vs. NCBI nr
Match: KAA0064621.1 (putative alpha-amylase 2 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 865 bits (2235), Expect = 1.15e-314
Identity = 416/470 (88.51%), Postives = 429/470 (91.28%), Query Frame = 0

Query: 62  RDGTPHPAPLRLVLAKNLEPTRLQFNCLIANGSNRQNRFPEPAEPRTS------------ 121
           RDG+ HP PLRL LAKNLEPTRLQ NCLIANG  R    P   +PR              
Sbjct: 3   RDGSLHPTPLRLALAKNLEPTRLQINCLIANGLGR----PYLLQPRRQGFHKFLPSIMGD 62

Query: 122 NREVFDESYEQTDSGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSAWLP 181
           +  VFDE+YEQTD+GAVIRNGREILFQAF+WESHK DW  NLE KVHDIAKSGFTSAWLP
Sbjct: 63  HDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSAWLP 122

Query: 182 PPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAIADIVINHRVGTVVGHG 241
           PPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRA+ADIVINHRVGTVVGHG
Sbjct: 123 PPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHG 182

Query: 242 GMHNRFDGIPLPWNEYAVTSCSGGLGNRSTGDNFYGVPNIDHTQPFVRRDIIAWLKWLRS 301
           GMHNRFDGI LPW+E+AVTSCSGGLGNRSTGDNF+GVPNIDHTQPFVRRDIIAWLKWLRS
Sbjct: 183 GMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRS 242

Query: 302 SVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQRIIN 361
           SVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQRIIN
Sbjct: 243 SVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQRIIN 302

Query: 362 WIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGS 421
           WIDGTGQLSAAFDFTTKGVLQEAV+ QLWRL DC+GKPPGVMGWWPSRAVTFLDNHDTGS
Sbjct: 303 WIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHDTGS 362

Query: 422 TQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVKLIDIRKHQDINSRS 481
           TQAHWPFPANHIMEGYAYILTHPGIPTVFYDH Y+WGDSVHDEIVKLIDIRKHQDINSRS
Sbjct: 363 TQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDINSRS 422

Query: 482 SVKILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 519
           SVKILEARSDLYSAIIGE LCMKIGDGSWCP GTEWTLAT G RYAVWQK
Sbjct: 423 SVKILEARSDLYSAIIGENLCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 467

BLAST of Chy7G131980 vs. NCBI nr
Match: XP_011654252.1 (probable alpha-amylase 2 [Cucumis sativus] >XP_031739646.1 probable alpha-amylase 2 [Cucumis sativus])

HSP 1 Score: 860 bits (2221), Expect = 1.92e-313
Identity = 399/407 (98.03%), Postives = 403/407 (99.02%), Query Frame = 0

Query: 113 VFDESYEQTDSGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSAWLPPPT 172
           VFDESYE+TD+GAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSAWLPPPT
Sbjct: 7   VFDESYEKTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSAWLPPPT 66

Query: 173 HSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAIADIVINHRVGTVVGHGGMH 232
           HSIAPQGYLPQNLYSLNSSYGSEDMLK LLLKMKQCKVRAIADIVINHRVGTVVGHGGMH
Sbjct: 67  HSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVVGHGGMH 126

Query: 233 NRFDGIPLPWNEYAVTSCSGGLGNRSTGDNFYGVPNIDHTQPFVRRDIIAWLKWLRSSVG 292
           NRFDGI LPWNEYAVTSCSGGLGNRSTGDNF+GVPNIDHTQPFVRRDIIAWLKWLRSSVG
Sbjct: 127 NRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVG 186

Query: 293 FQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQRIINWID 352
           FQDFRFDFARGFSAKFVKEYIEA KPM SIGEYWDSCNYNGHDLDYNQDGHRQRIINWID
Sbjct: 187 FQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQRIINWID 246

Query: 353 GTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQA 412
           GTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQA
Sbjct: 247 GTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQA 306

Query: 413 HWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVKLIDIRKHQDINSRSSVK 472
           HWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIV+LIDIRKHQDINSRSSVK
Sbjct: 307 HWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVRLIDIRKHQDINSRSSVK 366

Query: 473 ILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 519
           ILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK
Sbjct: 367 ILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 413

BLAST of Chy7G131980 vs. NCBI nr
Match: TYK19971.1 (putative alpha-amylase 2 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 859 bits (2220), Expect = 2.47e-312
Identity = 416/474 (87.76%), Postives = 429/474 (90.51%), Query Frame = 0

Query: 62  RDGTPHPAPLRLVLAKNLEPTRLQFNCLIANGSNRQNRFPEPAEPRTS------------ 121
           RDG+ HP PLRL LAKNLEPTRLQ NCLIANG  R    P   +PR              
Sbjct: 3   RDGSLHPTPLRLALAKNLEPTRLQINCLIANGLGR----PYLLQPRRQGFHKFLPSIMGD 62

Query: 122 NREVFDESYEQTDSGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSAWLP 181
           +  VFDE+YEQTD+GAVIRNGREILFQAF+WESHK DW  NLE KVHDIAKSGFTSAWLP
Sbjct: 63  HDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSAWLP 122

Query: 182 PPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAIADIVINHRVGTVVGHG 241
           PPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRA+ADIVINHRVGTVVGHG
Sbjct: 123 PPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHG 182

Query: 242 GMHNRFDGIPLPWNEYAVTSCSGGLGNRSTGDNFYGVPNIDHTQPFVRRDIIAWLKWLRS 301
           GMHNRFDGI LPW+E+AVTSCSGGLGNRSTGDNF+GVPNIDHTQPFVRRDIIAWLKWLRS
Sbjct: 183 GMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRS 242

Query: 302 SVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQ----DGHRQ 361
           SVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQ    DGHRQ
Sbjct: 243 SVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQALNADGHRQ 302

Query: 362 RIINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNH 421
           RIINWIDGTGQLSAAFDFTTKGVLQEAV+ QLWRL DC+GKPPGVMGWWPSRAVTFLDNH
Sbjct: 303 RIINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNH 362

Query: 422 DTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVKLIDIRKHQDI 481
           DTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDH Y+WGDSVHDEIVKLIDIRKHQDI
Sbjct: 363 DTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDI 422

Query: 482 NSRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 519
           NSRSSVKILEARSDLYSAIIGE LCMKIGDGSWCP GTEWTLAT G RYAVWQK
Sbjct: 423 NSRSSVKILEARSDLYSAIIGENLCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 471

BLAST of Chy7G131980 vs. NCBI nr
Match: KGN55476.1 (hypothetical protein Csa_012097 [Cucumis sativus])

HSP 1 Score: 850 bits (2197), Expect = 7.76e-310
Identity = 397/407 (97.54%), Postives = 400/407 (98.28%), Query Frame = 0

Query: 113 VFDESYEQTDSGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSAWLPPPT 172
           VFDESYE+TD+GAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSAWLPPPT
Sbjct: 7   VFDESYEKTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSAWLPPPT 66

Query: 173 HSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAIADIVINHRVGTVVGHGGMH 232
           HSIAPQGYLPQNLYSLNSSYGSEDMLK LLLKMKQCKVRAIADIVINHRVGTVVGHGGMH
Sbjct: 67  HSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVVGHGGMH 126

Query: 233 NRFDGIPLPWNEYAVTSCSGGLGNRSTGDNFYGVPNIDHTQPFVRRDIIAWLKWLRSSVG 292
           NRFDGI LPWNEYAVTSCSGGLGNRSTGDNF+GVPNIDHTQPFVRRDIIAWLKWLRSSVG
Sbjct: 127 NRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVG 186

Query: 293 FQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQRIINWID 352
           FQDFRFDFARGFSAKFVKEYIEA KPM SIGEYWDSCNYNGHDLDYNQDGHRQRIINWID
Sbjct: 187 FQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQRIINWID 246

Query: 353 GTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQA 412
           GTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQA
Sbjct: 247 GTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQA 306

Query: 413 HWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVKLIDIRKHQDINSRSSVK 472
           HWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEI   IDIRKHQDINSRSSVK
Sbjct: 307 HWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEI---IDIRKHQDINSRSSVK 366

Query: 473 ILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 519
           ILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK
Sbjct: 367 ILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 410

BLAST of Chy7G131980 vs. NCBI nr
Match: XP_008452915.1 (PREDICTED: probable alpha-amylase 2 isoform X2 [Cucumis melo])

HSP 1 Score: 820 bits (2119), Expect = 1.65e-297
Identity = 385/407 (94.59%), Postives = 395/407 (97.05%), Query Frame = 0

Query: 113 VFDESYEQTDSGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSAWLPPPT 172
           VFDE+YEQTD+GAVIRNGREILFQAF+WESHK DW  NLE KVHDIAKSGFTSAWLPPPT
Sbjct: 32  VFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSAWLPPPT 91

Query: 173 HSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAIADIVINHRVGTVVGHGGMH 232
           HSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRA+ADIVINHRVGTVVGHGGMH
Sbjct: 92  HSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHGGMH 151

Query: 233 NRFDGIPLPWNEYAVTSCSGGLGNRSTGDNFYGVPNIDHTQPFVRRDIIAWLKWLRSSVG 292
           NRFDGI LPW+E+AVTSCSGGLGNRSTGDNF+GVPNIDHTQPFVRRDIIAWLKWLRSSVG
Sbjct: 152 NRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVG 211

Query: 293 FQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQRIINWID 352
           FQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQRIINWID
Sbjct: 212 FQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQRIINWID 271

Query: 353 GTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQA 412
           GTGQLSAAFDFTTKGVLQEAV+ QLWRL DC+GKPPGVMGWWPSRAVTFLDNHDTGSTQA
Sbjct: 272 GTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHDTGSTQA 331

Query: 413 HWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVKLIDIRKHQDINSRSSVK 472
           HWPFPANHIMEGYAYILTHPGIPTVFYDH Y+WGDSVHDEIVKLIDIRKHQDINSRSSVK
Sbjct: 332 HWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDINSRSSVK 391

Query: 473 ILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 519
           ILEARSDLYSAIIGE LCMKIGDGSWCP GTEWTLAT G RYAVWQK
Sbjct: 392 ILEARSDLYSAIIGENLCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 437

BLAST of Chy7G131980 vs. TAIR 10
Match: AT1G76130.1 (alpha-amylase-like 2 )

HSP 1 Score: 726.1 bits (1873), Expect = 2.0e-209
Identity = 320/407 (78.62%), Postives = 371/407 (91.15%), Query Frame = 0

Query: 113 VFDESYEQTDSGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSAWLPPPT 172
           VFDE  +QTD G VIR+GRE++ QA++WESHKYDWW NL+ KV DIAKSGFTSAWLPPP+
Sbjct: 7   VFDECNDQTDIGRVIRDGREVILQAYNWESHKYDWWRNLDGKVPDIAKSGFTSAWLPPPS 66

Query: 173 HSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAIADIVINHRVGTVVGHGGMH 232
            S+AP+GYLPQ+LYSLNS+YGSE +LK+LL KMKQ KVRA+ADIVINHRVGT  GHGGM+
Sbjct: 67  QSLAPEGYLPQDLYSLNSAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTRGHGGMY 126

Query: 233 NRFDGIPLPWNEYAVTSCSGGLGNRSTGDNFYGVPNIDHTQPFVRRDIIAWLKWLRSSVG 292
           NR+DGI LPW+E+AVTSC+GGLGNRSTGDNF GVPN+DHTQ FVR+DII WL+WLR++VG
Sbjct: 127 NRYDGISLPWDEHAVTSCTGGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRWLRNTVG 186

Query: 293 FQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQRIINWID 352
           FQDFRFDFARG+SA +VKEYI AAKP+FS+GE WDSCNYNGH LDYNQD HRQRII+WID
Sbjct: 187 FQDFRFDFARGYSANYVKEYIGAAKPLFSVGECWDSCNYNGHGLDYNQDSHRQRIISWID 246

Query: 353 GTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQA 412
            TGQ+SAAFDFTTKG+LQEAV+ Q WRL D QGKPPGVMGWWPSRAVTFLDNHDTGSTQA
Sbjct: 247 ATGQISAAFDFTTKGILQEAVKGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHDTGSTQA 306

Query: 413 HWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVKLIDIRKHQDINSRSSVK 472
           HWPFP++H+MEGYAYILTHPGIP VFYDH Y+WG S+HD+IVKLIDIR+ QDI+SRS+V+
Sbjct: 307 HWPFPSHHVMEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVR 366

Query: 473 ILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 520
           +L+A S+LY+AI+GEK+CMK+GDGSWCPSG +WTLATSG RYAVW K
Sbjct: 367 VLKAESNLYAAIVGEKICMKLGDGSWCPSGRDWTLATSGHRYAVWHK 413

BLAST of Chy7G131980 vs. TAIR 10
Match: AT1G69830.1 (alpha-amylase-like 3 )

HSP 1 Score: 420.6 bits (1080), Expect = 1.8e-117
Identity = 196/397 (49.37%), Postives = 272/397 (68.51%), Query Frame = 0

Query: 129 NGREILFQAFSWESHKYD-WWENLESKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYS 188
           +G EIL Q F+WES+K   W+  L+ K  ++A  GFT  WLPPPT S++P+GY+P++LY+
Sbjct: 493 SGFEILCQGFNWESNKSGRWYLELQEKADELASLGFTVLWLPPPTESVSPEGYMPKDLYN 552

Query: 189 LNSSYGSEDMLKALLLKMKQCKVRAIADIVINHRVGTVVGHGGMHNRFDGIPLPWNEYAV 248
           LNS YG+ D LK  + K  +  ++ + D V+NHR        G+ N F G  L W++ AV
Sbjct: 553 LNSRYGTIDELKDTVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGG-RLNWDDRAV 612

Query: 249 TSCS---GGLGNRSTGDNFYGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGF 308
            +      G GN+S+GDNF+  PNIDH+Q FVR+DI  WL W+   VG+  +R DF RGF
Sbjct: 613 VADDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWMMEEVGYDGWRLDFVRGF 672

Query: 309 SAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQRIINWIDGTGQLSAAFDFT 368
              +VK+Y++A+KP F++GEYWDS +Y   ++DYNQD HRQRI++WI+ T   + AFD T
Sbjct: 673 WGGYVKDYMDASKPYFAVGEYWDSLSYTYGEMDYNQDAHRQRIVDWINATSGAAGAFDVT 732

Query: 369 TKGVLQEAVRR-QLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIME 428
           TKG+L  A+++ + WRL D +GKPPGV+GWWPSRAVTF++NHDTGSTQ HW FP    M+
Sbjct: 733 TKGILHTALQKCEYWRLSDPKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPEGKEMQ 792

Query: 429 GYAYILTHPGIPTVFYDHLYEWGDSVHDEIVKLIDIRKHQDINSRSSVKILEARSDLYSA 488
           GYAYILTHPG P VF+DH++      H EI  L+ +R  Q ++ RS V I ++  D+Y+A
Sbjct: 793 GYAYILTHPGTPAVFFDHIF---SDYHSEIAALLSLRNRQKLHCRSEVNIDKSERDVYAA 852

Query: 489 IIGEKLCMKIGDGSW-CPSGTE-WTLATSGWRYAVWQ 519
           II EK+ MKIG G +  P+G++ W++A  G  Y VW+
Sbjct: 853 IIDEKVAMKIGPGHYEPPNGSQNWSVAVEGRDYKVWE 885

BLAST of Chy7G131980 vs. TAIR 10
Match: AT4G25000.1 (alpha-amylase-like )

HSP 1 Score: 351.3 bits (900), Expect = 1.3e-96
Identity = 183/403 (45.41%), Postives = 253/403 (62.78%), Query Frame = 0

Query: 133 ILFQAFSWESHKYD--WWENLESKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLNS 192
           +LFQ+F+WES K +  ++ +L + + DIA +G T  WLPPP+ S+AP+GYLP  LY LNS
Sbjct: 27  LLFQSFNWESWKKEGGFYNSLHNSIDDIANAGITHLWLPPPSQSVAPEGYLPGKLYDLNS 86

Query: 193 S-YGSEDMLKALLLKMKQCKVRAIADIVINHRVGTVVGHGGMHNRFDG----IPLPWNEY 252
           S YGSE  LK+L+  + Q  ++A+ADIVINHR          +  F+G      L W+  
Sbjct: 87  SKYGSEAELKSLIKALNQKGIKALADIVINHRTAERKDDKCGYCYFEGGTSDDRLDWDPS 146

Query: 253 AVTSCS---GGLGNRSTGDNFYGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFAR 312
            V        G GN  TG +F G P+IDH  P V++++  W+ WL++ +GF  +RFD+ R
Sbjct: 147 FVCRNDPKFPGTGNLDTGGDFDGAPDIDHLNPRVQKELSEWMNWLKTEIGFHGWRFDYVR 206

Query: 313 GFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGH-DLDYNQDGHRQRIINWI-DGTGQLSAA 372
           G+++   K Y++   P F++GE WD   Y G   LDY+Q+ HR  +  WI +  G +  A
Sbjct: 207 GYASSITKLYVQNTSPDFAVGEKWDDMKYGGDGKLDYDQNEHRSGLKQWIEEAGGGVLTA 266

Query: 373 FDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANH 432
           FDFTTKG+LQ AV+ +LWRL+D QGKPPG++G  P  AVTF+DNHDT  T   W FP++ 
Sbjct: 267 FDFTTKGILQSAVKGELWRLKDSQGKPPGMIGIMPGNAVTFIDNHDTFRT---WVFPSDK 326

Query: 433 IMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVKLIDIRKHQDINSRSSVKILEARSDL 492
           ++ GY YILTHPG P +FY+H  EWG  + + I KL+ IR    I S SSV I  A +DL
Sbjct: 327 VLLGYVYILTHPGTPCIFYNHYIEWG--LKESISKLVAIRNKNGIGSTSSVTIKAAEADL 386

Query: 493 YSAIIGEKLCMKIGD----GSWCPSGTEWTLATSGWRYAVWQK 520
           Y A+I +K+ MKIG     G+  PS   + LA SG  +AVW+K
Sbjct: 387 YLAMIDDKVIMKIGPKQDVGTLVPS--NFALAYSGLDFAVWEK 422

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LFG12.8e-20878.62Probable alpha-amylase 2 OS=Arabidopsis thaliana OX=3702 GN=AMY2 PE=2 SV=1[more]
Q94A412.5e-11649.37Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AMY3 PE=1 SV=1[more]
P178594.2e-10345.52Alpha-amylase OS=Vigna mungo OX=3915 GN=AMY1.1 PE=2 SV=1[more]
P279327.2e-10345.05Alpha-amylase isozyme 3A OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.2 PE=2... [more]
P279347.2e-10345.79Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.4 PE=2... [more]
Match NameE-valueIdentityDescription
A0A5A7VG022.6e-24988.51Alpha-amylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G003440... [more]
A0A5D3D8R21.4e-24787.76Alpha-amylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00850 ... [more]
A0A0A0L5R61.5e-24497.54Alpha-amylase OS=Cucumis sativus OX=3659 GN=Csa_4G652880 PE=3 SV=1[more]
A0A1S3BW591.7e-23594.59Alpha-amylase OS=Cucumis melo OX=3656 GN=LOC103493790 PE=3 SV=1[more]
E5GCD39.1e-23493.67Alpha-amylase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
KAA0064621.11.15e-31488.51putative alpha-amylase 2 isoform X2 [Cucumis melo var. makuwa][more]
XP_011654252.11.92e-31398.03probable alpha-amylase 2 [Cucumis sativus] >XP_031739646.1 probable alpha-amylas... [more]
TYK19971.12.47e-31287.76putative alpha-amylase 2 isoform X1 [Cucumis melo var. makuwa][more]
KGN55476.17.76e-31097.54hypothetical protein Csa_012097 [Cucumis sativus][more]
XP_008452915.11.65e-29794.59PREDICTED: probable alpha-amylase 2 isoform X2 [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT1G76130.12.0e-20978.62alpha-amylase-like 2 [more]
AT1G69830.11.8e-11749.37alpha-amylase-like 3 [more]
AT4G25000.11.3e-9645.41alpha-amylase-like [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006046Alpha amylasePRINTSPR00110ALPHAAMYLASEcoord: 209..220
score: 52.04
coord: 293..304
score: 35.54
coord: 396..408
score: 53.54
coord: 147..164
score: 21.79
IPR006047Glycosyl hydrolase, family 13, catalytic domainSMARTSM00642aamycoord: 132..472
e-value: 9.4E-24
score: 94.9
IPR006047Glycosyl hydrolase, family 13, catalytic domainPFAMPF00128Alpha-amylasecoord: 153..220
e-value: 2.4E-8
score: 33.9
IPR012850Alpha-amylase, C-terminal beta-sheetSMARTSM00810alpha-amyl_c2coord: 461..519
e-value: 6.1E-28
score: 108.8
IPR012850Alpha-amylase, C-terminal beta-sheetPFAMPF07821Alpha-amyl_C2coord: 463..518
e-value: 1.9E-21
score: 76.1
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 132..464
e-value: 1.7E-117
score: 394.8
NoneNo IPR availablePANTHERPTHR43447ALPHA-AMYLASEcoord: 127..519
NoneNo IPR availablePANTHERPTHR43447:SF18ALPHA-AMYLASE 2-RELATEDcoord: 127..519
NoneNo IPR availableCDDcd11314AmyAc_arch_bac_plant_AmyAcoord: 133..471
e-value: 7.81312E-166
score: 469.779
NoneNo IPR availableSUPERFAMILY51011Glycosyl hydrolase domaincoord: 465..519
IPR013780Glycosyl hydrolase, all-betaGENE3D2.60.40.1180coord: 465..519
e-value: 1.7E-18
score: 68.5
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 139..451

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy7G131980.1Chy7G131980.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004556 alpha-amylase activity
molecular_function GO:0103025 alpha-amylase activity (releasing maltohexaose)
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0003824 catalytic activity
molecular_function GO:0043169 cation binding