Chy7G129860 (gene) Cucumber (hystrix) v1

Overview


Name	Chy7G129860
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	low-temperature-induced cysteine proteinase-like
Location	chrH07: 1328055 .. 1332167 (+)
RNA-Seq Expression	Chy7G129860
Synteny	Chy7G129860

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGACCTTCCAATCCTCCCCGATCATTGCTCTTCTTTTCTTCCTCTTCATTGCTTTATCAACCGCCTCCCCTTCTTCCATCATTCCCCACAGGACGGACGACGAGGTCATGGCGCTCTACGACCAGTGGCGTGCCAAGCACGGGAAGCTCCACAACAACTTGGGAGCTGAGCCTGAAGATCGTTTCCACATCTTCAAGGACAATCTTAAGTTCATCGATGAAATCAACGCCCAAAATTTGCCCTACCGATTAGGTTTGAATGTTTTCGCAGATCTGACCAACGAGGAGTACCGATCTAGGTACTTGGGAGGTAAATTCGCCTCTGGTTCGAGGAGAAACAGGACGAGCAACCGGTATCTACCTCGCCTTGGAGATGACCTCCCAGACTCTATTGATTGGAGGGCGAAAGGAGCCGTCGTTGCTGTTAAAGACCAGGGTAGCTGTGGTATGTTCCATTTTTCTCGCCTTTTTTTTTTTTGGTTTCCTATTTACTTGTTTAACGTCTTTCCTATTTTTACTCTTTGATTTTGTGTCTTTTTCTTTAACTTTCTTCACTCTTTTGTATATTTGGAGCACACACACACACACTAATTTGTCTTTTCAGTAATCAGTATTAATTGCAATGAATTTTAATTATTTGCTTTAGTTTATTATGATCCATTGGTGTGATGGTTTTTAAAAGGTCGACCGATGATTGATCATTGTAAGAGTGTTGATTGTCTGTTTCTTAATTTTGGTTTCAATGACTAAAAATTGTTGTTGCTGCCTTGTTTTTCCTTCTTTAGTATACAGTAGATTCTGTTTTGATACTTTTAAAATATATCTATCCATCGAATTTTATTTAGAAGAATTTTTACAGACTGACCTTTTGATGATCTTTCTATGTGTTTGGTACTAATTTAATTAACCTGCTTTCCAATTCAAAATCAACTAACAAGGAAAGAGGTAACTCATACCTGTTATAAGAACGTTTCATTGATTTTTCAATGAGTTGGAATTGAAATTTTGATTTAGAAGAATTGAGGGTTGTCCGTTTGGTGTAGAGTGTTTTCACTTGCTTTTCAGGTGGAGCGCTTTTTGAGTTGCTCTGCCTTATCTTTAACTCGAACTGGTCCCGCCATATGGCTTTTTGGTCTCCATCTAACTTCAATCATGAGATATTTTTTAGAGATTTAATCATGATTTTTTCTTTGATATATTAATCATGATATTTTTTAAATTTGAATGTGGACTTTGTTTTTTTTAACACTTATCTTTATATTAATTGTTAGTTATATGTCAGTTTTCTAAAAATATTATTGCCACATGACGCCTTAATACAAGACCAACAAGTTGTTCTAAACCAAATTGAAGAACTTGTTAAATTTTTATGATGCTTTAATTTTAGGCAATTGGCAGCTTATCTGAAATATTATTTAATCATTGAGTTTAAATTCTATATTGGTTGACTTTCCCTCCTTTCATTTAACTACTTTCCAAGCCCCCTTTTTTTTCTTTTGAAAATTACTATTTTTCCAATTTCTAGCACAATGTACGCACAAGAAAGATCTTTTGATACGAATGTGGAATACTATTTCAAGAAAATGATCAGGTATAATGTTGCGTTGGAATTATATTTTAAAAATAAGATAAAAAAGAAAATTGAAGTATTGAAATAATCTCTTTAAAATATGGAAGAACTTATATGGATAATTTGAATATACATTTTTAAAATGTAAGGATTATGGGAACTTAATAATCTTTATGAAAGAAAAGAGACAAAAGAGAAAAAAGAGAATTTGAAATCAAGTTATTTCTATACTTGTACGGGTCTTTTCATCATTATTTGTTAAACATTTTGATCTTAATCATAAATTTATTTCAATAGCAGCATCTTAACTATCATGACACAGTACGAATATTAGCTCAATAGAAATTGAGTATTAATTTGATTAATTAAAATTATTATATTGTCAAGAGCAATAATTCAAGTATACTTGTATTGATAGTTCATTCCACCTCAAACTTGGTTTTTTAAGATAAATCCTCTTATATTGAAAATTTATTATAAAGGATGTATAGTAATTTTAAAAATCTTTGCAGTTATTTCTCTTAAAAAAAACAGTAATTTAGCATAGTAGCGTTAAAGTTGAATTTCAAATACTTATCAATTATTAGATCTCTTATCGATTTAACTATTTTATATATAGCAACAAGTAAAGGAACTGTTTGAAGGGATATGCATAATAAATTAATAATTACATACAAAGTAGATATTTTTATGGTGCAATGCCCATAGATGAGAAGGTGGGACCTTGGGCTTGTTCGGTTTCTGATTGAAAGCTCTTTGGTAGGCCAAATGTTTGTTTGCTGTCAGTGGAATTTGTTTTCCAATTTAGCCTTAACTATTTATAGATTTTCAGTTAACACTAATTCTAACACATATGACTATCAATTCTACACTTTATGCCCTTCAAAATTAGGATTGGCTGATTGAATAAGTTGAAACTTTTCTGTAAACTTCCAAGAGAGAGTATATACTTACTTGACATATTTTCTCCAAAAAATTTATCAACCGACTTAGATTATTGTATTTAAATGTATCATTAATCAACTACATCTATTAATAAATAAAGTACAATTCAATTTTTTTTGAACTCACATGTCGGATGCGGATTAGTCAAAACATATTGACATGTATTTAGCCCAGTCACTGTTTCTAAACTATACTCTCAAGTATAAGTAGGCTAATTGTTGAAGTTGGTATGAAGGGAGTTGCTGGGCGTTCTCAACTGTAGCTTCCGTTGAAGCCATAAACCAGATTGTTACAGGCGACTTGGTAGCATTATCTGAACAGGAGCTGGTGGATTGTGACAGATCTTACAACGAAGGTTGCAATGGTGGCTTAATGGACTATGCCTTCGAGTTCATCATCGACAATGGCGGCCTTGACACTGAAGAAGATTATCCTTACAATGGCTTTGATAGCTCCTGCATCCAATATAAGGTCTCTATTACCACTCAACTATTTTCTCTTTCTCTCTCTCTCTCTCTGAGTACCAGAAGTTCATTTTTGATATCTGATTACCTTTTCTTCTATTGTGTTTTGCCTCCTCTAGAAAAATGCCAAGGTCGTTGCAATTGATAGTTATGAAGACGTCCCTGTAAACAATGAGAAAGCACTGCAAAAAGCAGTGTCAAAACAGGTTGTGTCTGTTGCCATTGAAGGAGGTGGTAGAAGTTTCCAGCTATACCAATCGGTATAACCTCTATTTCTATGAAGTTTTTGTTTTTTCTTACTTCAACAAGAAAAGGCTCTAATTTGTTGTATGTGCATGCGTTTAGGGTATATTCACTGGAAGGTGTGGAACAGATTTGGACCATGGTGTAAATGTGGTTGGATATGGTTCTAAAGGAGGCGTTGATTACTGGATTGTGAGAAACTCGTGGGGTGGGAGCTGGGGTGAAAGTGGCTACGTCAGGATGCAACGTAATATCGCTTCCCCAACCGGTCTCTGTGGGATTGCAATGGAGCCTTCCTACCCCACTAAAACCGGACCAAACCCACCAAATCCAGGCCCAACTCCTCCAAGTCCGGTGAAACCCCCATCTGTATGTGATGAATATTACACCTGTCCCGCCGCTGAAACTTGCTGCTGTATCTTCCAATTCTCAAACTTGTGTCTTGAATGGGGATGCTGCCCACTTGAAAGCGCCACCTGCTGTGATGACCATTACAGCTGCTGCCCCCACGATTACCCCATCTGTAACGTCCGCGCTGGAACCTGCTCGAAGGTACGTGCCTTCAGCTTCCAATTTATTGTGTCTATCTTATCGAGATTCTCAAATTGAAAAAAATCAAGTGACCGCATACTTATAAAATGATGAGTTAAATAGACCCATCTTCAGGAGTCATTAGGAAAAAAAAAAAGATTAAGAAAAGACCACTACTTATAATTGACGGTGTCAAAACGCCACAGGTTGATGTTTGAAGAAGAAAACTTAACTTATTTTTGTGTTGTGCAGAGCAAGAATGATATTTTCGGAGTGAAGGCGATGAGGCGCACTGCAGCTGCAGCCAGGCCATCGTGGGCACGTAGAGATGTGACTGTTGGAAAGAGCAGTGCTTAA

mRNA sequence

ATGGGGACCTTCCAATCCTCCCCGATCATTGCTCTTCTTTTCTTCCTCTTCATTGCTTTATCAACCGCCTCCCCTTCTTCCATCATTCCCCACAGGACGGACGACGAGGTCATGGCGCTCTACGACCAGTGGCGTGCCAAGCACGGGAAGCTCCACAACAACTTGGGAGCTGAGCCTGAAGATCGTTTCCACATCTTCAAGGACAATCTTAAGTTCATCGATGAAATCAACGCCCAAAATTTGCCCTACCGATTAGGTTTGAATGTTTTCGCAGATCTGACCAACGAGGAGTACCGATCTAGGTACTTGGGAGGTAAATTCGCCTCTGGTTCGAGGAGAAACAGGACGAGCAACCGGTATCTACCTCGCCTTGGAGATGACCTCCCAGACTCTATTGATTGGAGGGCGAAAGGAGCCGTCGTTGCTGTTAAAGACCAGGGTAGCTGTGGGAGTTGCTGGGCGTTCTCAACTGTAGCTTCCGTTGAAGCCATAAACCAGATTGTTACAGGCGACTTGGTAGCATTATCTGAACAGGAGCTGGTGGATTGTGACAGATCTTACAACGAAGGTTGCAATGGTGGCTTAATGGACTATGCCTTCGAGTTCATCATCGACAATGGCGGCCTTGACACTGAAGAAGATTATCCTTACAATGGCTTTGATAGCTCCTGCATCCAATATAAGAAAAATGCCAAGGTCGTTGCAATTGATAGTTATGAAGACGTCCCTGTAAACAATGAGAAAGCACTGCAAAAAGCAGTGTCAAAACAGGTTGTGTCTGTTGCCATTGAAGGAGGTGGTAGAAGTTTCCAGCTATACCAATCGGGTATATTCACTGGAAGGTGTGGAACAGATTTGGACCATGGTGTAAATGTGGTTGGATATGGTTCTAAAGGAGGCGTTGATTACTGGATTGTGAGAAACTCGTGGGGTGGGAGCTGGGGTGAAAGTGGCTACGTCAGGATGCAACGTAATATCGCTTCCCCAACCGGTCTCTGTGGGATTGCAATGGAGCCTTCCTACCCCACTAAAACCGGACCAAACCCACCAAATCCAGGCCCAACTCCTCCAAGTCCGGTGAAACCCCCATCTGTATGTGATGAATATTACACCTGTCCCGCCGCTGAAACTTGCTGCTGTATCTTCCAATTCTCAAACTTGTGTCTTGAATGGGGATGCTGCCCACTTGAAAGCGCCACCTGCTGTGATGACCATTACAGCTGCTGCCCCCACGATTACCCCATCTGTAACGTCCGCGCTGGAACCTGCTCGAAGAGCAAGAATGATATTTTCGGAGTGAAGGCGATGAGGCGCACTGCAGCTGCAGCCAGGCCATCGTGGGCACGTAGAGATGTGACTGTTGGAAAGAGCAGTGCTTAA

Coding sequence (CDS)

ATGGGGACCTTCCAATCCTCCCCGATCATTGCTCTTCTTTTCTTCCTCTTCATTGCTTTATCAACCGCCTCCCCTTCTTCCATCATTCCCCACAGGACGGACGACGAGGTCATGGCGCTCTACGACCAGTGGCGTGCCAAGCACGGGAAGCTCCACAACAACTTGGGAGCTGAGCCTGAAGATCGTTTCCACATCTTCAAGGACAATCTTAAGTTCATCGATGAAATCAACGCCCAAAATTTGCCCTACCGATTAGGTTTGAATGTTTTCGCAGATCTGACCAACGAGGAGTACCGATCTAGGTACTTGGGAGGTAAATTCGCCTCTGGTTCGAGGAGAAACAGGACGAGCAACCGGTATCTACCTCGCCTTGGAGATGACCTCCCAGACTCTATTGATTGGAGGGCGAAAGGAGCCGTCGTTGCTGTTAAAGACCAGGGTAGCTGTGGGAGTTGCTGGGCGTTCTCAACTGTAGCTTCCGTTGAAGCCATAAACCAGATTGTTACAGGCGACTTGGTAGCATTATCTGAACAGGAGCTGGTGGATTGTGACAGATCTTACAACGAAGGTTGCAATGGTGGCTTAATGGACTATGCCTTCGAGTTCATCATCGACAATGGCGGCCTTGACACTGAAGAAGATTATCCTTACAATGGCTTTGATAGCTCCTGCATCCAATATAAGAAAAATGCCAAGGTCGTTGCAATTGATAGTTATGAAGACGTCCCTGTAAACAATGAGAAAGCACTGCAAAAAGCAGTGTCAAAACAGGTTGTGTCTGTTGCCATTGAAGGAGGTGGTAGAAGTTTCCAGCTATACCAATCGGGTATATTCACTGGAAGGTGTGGAACAGATTTGGACCATGGTGTAAATGTGGTTGGATATGGTTCTAAAGGAGGCGTTGATTACTGGATTGTGAGAAACTCGTGGGGTGGGAGCTGGGGTGAAAGTGGCTACGTCAGGATGCAACGTAATATCGCTTCCCCAACCGGTCTCTGTGGGATTGCAATGGAGCCTTCCTACCCCACTAAAACCGGACCAAACCCACCAAATCCAGGCCCAACTCCTCCAAGTCCGGTGAAACCCCCATCTGTATGTGATGAATATTACACCTGTCCCGCCGCTGAAACTTGCTGCTGTATCTTCCAATTCTCAAACTTGTGTCTTGAATGGGGATGCTGCCCACTTGAAAGCGCCACCTGCTGTGATGACCATTACAGCTGCTGCCCCCACGATTACCCCATCTGTAACGTCCGCGCTGGAACCTGCTCGAAGAGCAAGAATGATATTTTCGGAGTGAAGGCGATGAGGCGCACTGCAGCTGCAGCCAGGCCATCGTGGGCACGTAGAGATGTGACTGTTGGAAAGAGCAGTGCTTAA

Protein sequence

MGTFQSSPIIALLFFLFIALSTASPSSIIPHRTDDEVMALYDQWRAKHGKLHNNLGAEPEDRFHIFKDNLKFIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTSNRYLPRLGDDLPDSIDWRAKGAVVAVKDQGSCGSCWAFSTVASVEAINQIVTGDLVALSEQELVDCDRSYNEGCNGGLMDYAFEFIIDNGGLDTEEDYPYNGFDSSCIQYKKNAKVVAIDSYEDVPVNNEKALQKAVSKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGSKGGVDYWIVRNSWGGSWGESGYVRMQRNIASPTGLCGIAMEPSYPTKTGPNPPNPGPTPPSPVKPPSVCDEYYTCPAAETCCCIFQFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRAGTCSKSKNDIFGVKAMRRTAAAARPSWARRDVTVGKSSA*

Homology

[Close]

BLAST of Chy7G129860 vs. ExPASy Swiss-Prot
Match: P43297 (Cysteine proteinase RD21A OS=Arabidopsis thaliana OX=3702 GN=RD21A PE=1 SV=1)

HSP 1 Score: 610.5 bits (1573), Expect = 1.5e-173
Identity = 290/454 (63.88%), Postives = 349/454 (76.87%), Query Frame = 0


Query: 8   PIIALLFFLFIALSTASPSSIIPH-----------RTDDEVMALYDQWRAKHGKLHN-NL 67
           P +A+LF   +A+S+A   SII +           R++ EVM++Y+ W  KHGK  + N 
Sbjct: 6   PTMAILFLAMVAVSSAVDMSIISYDEKHGVSTTGGRSEAEVMSIYEAWLVKHGKAQSQNS 65

Query: 68  GAEPEDRFHIFKDNLKFIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNR 127
             E + RF IFKDNL+F+DE N +NL YRLGL  FADLTN+EYRS+YLG K      R R
Sbjct: 66  LVEKDRRFEIFKDNLRFVDEHNEKNLSYRLGLTRFADLTNDEYRSKYLGAKMEKKGER-R 125

Query: 128 TSNRYLPRLGDDLPDSIDWRAKGAVVAVKDQGSCGSCWAFSTVASVEAINQIVTGDLVAL 187
           TS RY  R+GD+LP+SIDWR KGAV  VKDQG CGSCWAFST+ +VE INQIVTGDL+ L
Sbjct: 126 TSLRYEARVGDELPESIDWRKKGAVAEVKDQGGCGSCWAFSTIGAVEGINQIVTGDLITL 185

Query: 188 SEQELVDCDRSYNEGCNGGLMDYAFEFIIDNGGLDTEEDYPYNGFDSSCIQYKKNAKVVA 247
           SEQELVDCD SYNEGCNGGLMDYAFEFII NGG+DT++DYPY G D +C Q +KNAKVV 
Sbjct: 186 SEQELVDCDTSYNEGCNGGLMDYAFEFIIKNGGIDTDKDYPYKGVDGTCDQIRKNAKVVT 245

Query: 248 IDSYEDVPVNNEKALQKAVSKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGY 307
           IDSYEDVP  +E++L+KAV+ Q +S+AIE GGR+FQLY SGIF G CGT LDHGV  VGY
Sbjct: 246 IDSYEDVPTYSEESLKKAVAHQPISIAIEAGGRAFQLYDSGIFDGSCGTQLDHGVVAVGY 305

Query: 308 GSKGGVDYWIVRNSWGGSWGESGYVRMQRNIASPTGLCGIAMEPSYPTKTGPNPPNPGPT 367
           G++ G DYWIVRNSWG SWGESGY+RM RNIAS +G CGIA+EPSYP K G NPPNPGP+
Sbjct: 306 GTENGKDYWIVRNSWGKSWGESGYLRMARNIASSSGKCGIAIEPSYPIKNGENPPNPGPS 365

Query: 368 PPSPVKPPSVCDEYYTCPAAETCCCIFQFSNLCLEWGCCPLESATCCDDHYSCCPHDYPI 427
           PPSP+KPP+ CD YYTCP + TCCC+F++   C  WGCCPLE+ATCCDD+YSCCPH+YP+
Sbjct: 366 PPSPIKPPTQCDSYYTCPESNTCCCLFEYGKYCFAWGCCPLEAATCCDDNYSCCPHEYPV 425

Query: 428 CNVRAGTCSKSKNDIFGVKAMRRTAAAARPSWAR 450
           C++  GTC  SKN  F VKA++R  A   P W++
Sbjct: 426 CDLDQGTCLLSKNSPFSVKALKRKPAT--PFWSQ 456

[Close]

BLAST of Chy7G129860 vs. ExPASy Swiss-Prot
Match: Q9FMH8 (Probable cysteine protease RD21B OS=Arabidopsis thaliana OX=3702 GN=RD21B PE=1 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 2.7e-170
Identity = 290/463 (62.63%), Postives = 348/463 (75.16%), Query Frame = 0


Query: 1   MGTFQSSPIIALLFFLFIALSTASPSSIIPH-----------RTDDEVMALYDQWRAKHG 60
           MG  + SP+I LL    I +S A   SII +           R+D EV  +Y+ W  +HG
Sbjct: 1   MGFLKLSPMILLL--AMIGVSYAMDMSIISYDENHHITTETSRSDSEVERIYEAWMVEHG 60

Query: 61  KL---HNNLGAEPEDRFHIFKDNLKFIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGK 120
           K     N LGAE + RF IFKDNL+FIDE N +NL Y+LGL  FADLTNEEYRS YLG K
Sbjct: 61  KKKMNQNGLGAEKDQRFEIFKDNLRFIDEHNTKNLSYKLGLTRFADLTNEEYRSMYLGAK 120

Query: 121 FASGSRRNRTSNRYLPRLGDDLPDSIDWRAKGAVVAVKDQGSCGSCWAFSTVASVEAINQ 180
                R  +TS+RY  R+GD LPDS+DWR +GAV  VKDQGSCGSCWAFST+ +VE IN+
Sbjct: 121 --PTKRVLKTSDRYQARVGDALPDSVDWRKEGAVADVKDQGSCGSCWAFSTIGAVEGINK 180

Query: 181 IVTGDLVALSEQELVDCDRSYNEGCNGGLMDYAFEFIIDNGGLDTEEDYPYNGFDSSCIQ 240
           IVTGDL++LSEQELVDCD SYN+GCNGGLMDYAFEFII NGG+DTE DYPY   D  C Q
Sbjct: 181 IVTGDLISLSEQELVDCDTSYNQGCNGGLMDYAFEFIIKNGGIDTEADYPYKAADGRCDQ 240

Query: 241 YKKNAKVVAIDSYEDVPVNNEKALQKAVSKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDL 300
            +KNAKVV IDSYEDVP N+E +L+KA++ Q +SVAIE GGR+FQLY SG+F G CGT+L
Sbjct: 241 NRKNAKVVTIDSYEDVPENSEASLKKALAHQPISVAIEAGGRAFQLYSSGVFDGLCGTEL 300

Query: 301 DHGVNVVGYGSKGGVDYWIVRNSWGGSWGESGYVRMQRNIASPTGLCGIAMEPSYPTKTG 360
           DHGV  VGYG++ G DYWIVRNSWG  WGESGY++M RNI +PTG CGIAME SYP K G
Sbjct: 301 DHGVVAVGYGTENGKDYWIVRNSWGNRWGESGYIKMARNIEAPTGKCGIAMEASYPIKKG 360

Query: 361 PNPPNPGPTPPSPVKPPSVCDEYYTCPAAETCCCIFQFSNLCLEWGCCPLESATCCDDHY 420
            NPPNPGP+PPSP+KPP+ CD+Y++CP + TCCC++++   C  WGCCPLE+ATCCDD+ 
Sbjct: 361 QNPPNPGPSPPSPIKPPTTCDKYFSCPESNTCCCLYKYGKYCFGWGCCPLEAATCCDDNS 420

Query: 421 SCCPHDYPICNVRAGTCSKSKNDIFGVKAMRRTAAAARPSWAR 450
           SCCPH+YP+C+V  GTC  SKN  F VKA++RT A   P WA+
Sbjct: 421 SCCPHEYPVCDVNRGTCLMSKNSPFSVKALKRTPAI--PFWAK 457

[Close]

BLAST of Chy7G129860 vs. ExPASy Swiss-Prot
Match: P25776 (Oryzain alpha chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0650000 PE=1 SV=2)

HSP 1 Score: 579.3 bits (1492), Expect = 3.8e-164
Identity = 279/441 (63.27%), Postives = 335/441 (75.96%), Query Frame = 0


Query: 6   SSPIIALLFFLFIALSTASPSSI-IPHRTDDEVMALYDQWRAKHGKLHNNLGAEPEDRFH 65
           S  + A    L ++L+ A  S +    R+++E   LY +W+A+HGK +N +G E E R+ 
Sbjct: 4   SMALAAAALLLLLSLAAADMSIVSYGERSEEEARRLYAEWKAEHGKSYNAVG-EEERRYA 63

Query: 66  IFKDNLKFIDEINAQN----LPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTSNRY 125
            F+DNL++IDE NA        +RLGLN FADLTNEEYR  YLG +     R  + S+RY
Sbjct: 64  AFRDNLRYIDEHNAAADAGVHSFRLGLNRFADLTNEEYRDTYLGLR-NKPRRERKVSDRY 123

Query: 126 LPRLGDDLPDSIDWRAKGAVVAVKDQGSCGSCWAFSTVASVEAINQIVTGDLVALSEQEL 185
           L    + LP+S+DWR KGAV  +KDQG CGSCWAFS +A+VE INQIVTGDL++LSEQEL
Sbjct: 124 LAADNEALPESVDWRTKGAVAEIKDQGGCGSCWAFSAIAAVEGINQIVTGDLISLSEQEL 183

Query: 186 VDCDRSYNEGCNGGLMDYAFEFIIDNGGLDTEEDYPYNGFDSSCIQYKKNAKVVAIDSYE 245
           VDCD SYNEGCNGGLMDYAF+FII+NGG+DTE+DYPY G D  C   +KNAKVV IDSYE
Sbjct: 184 VDCDTSYNEGCNGGLMDYAFDFIINNGGIDTEDDYPYKGKDERCDVNRKNAKVVTIDSYE 243

Query: 246 DVPVNNEKALQKAVSKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGSKGG 305
           DV  N+E +LQKAV+ Q VSVAIE GGR+FQLY SGIFTG+CGT LDHGV  VGYG++ G
Sbjct: 244 DVTPNSETSLQKAVANQPVSVAIEAGGRAFQLYSSGIFTGKCGTALDHGVAAVGYGTENG 303

Query: 306 VDYWIVRNSWGGSWGESGYVRMQRNIASPTGLCGIAMEPSYPTKTGPNPPNPGPTPPSPV 365
            DYWIVRNSWG SWGESGYVRM+RNI + +G CGIA+EPSYP K G NPPNPGPTPPSP 
Sbjct: 304 KDYWIVRNSWGKSWGESGYVRMERNIKASSGKCGIAVEPSYPLKKGENPPNPGPTPPSPT 363

Query: 366 KPPSVCDEYYTCPAAETCCCIFQFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRA 425
            PP+VCD YYTCP + TCCCI+++   C  WGCCPLE ATCCDDHYSCCPH+YPICNV+ 
Sbjct: 364 PPPTVCDNYYTCPDSTTCCCIYEYGKYCYAWGCCPLEGATCCDDHYSCCPHEYPICNVQQ 423

Query: 426 GTCSKSKNDIFGVKAMRRTAA 442
           GTC  +K+    VKA++RT A
Sbjct: 424 GTCLMAKDSPLAVKALKRTLA 442

[Close]

BLAST of Chy7G129860 vs. ExPASy Swiss-Prot
Match: P25777 (Oryzain beta chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0670200 PE=1 SV=2)

HSP 1 Score: 525.8 bits (1353), Expect = 5.0e-148
Identity = 268/455 (58.90%), Postives = 319/455 (70.11%), Query Frame = 0


Query: 11  ALLFFLFIALSTASPS-SIIPHR------------TDDEVMALYDQWRAKH-GKLHNNLG 70
           A L  L +  +TA+P  SII +             T+ E  A YD W A++ G   N LG
Sbjct: 9   AFLLLLIVGAATAAPDMSIISYNAEHGARGLEEGPTEAEARAAYDLWLAENGGGSPNALG 68

Query: 71  AEPEDRFHIFKDNLKFIDEINA---QNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRR 130
            E E RF +F DNLKF+D  NA   +   +RLG+N FADLTNEE+R+ +LG K A  SR 
Sbjct: 69  GEHERRFLVFWDNLKFVDAHNARADERGGFRLGMNRFADLTNEEFRATFLGAKVAERSR- 128

Query: 131 NRTSNRYLPRLGDDLPDSIDWRAKGAVVAVKDQGSCGSCWAFSTVASVEAINQIVTGDLV 190
                RY     ++LP+S+DWR KGAV  VK+QG CGSCWAFS V++VE+INQ+VTG+++
Sbjct: 129 -AAGERYRHDGVEELPESVDWREKGAVAPVKNQGQCGSCWAFSAVSTVESINQLVTGEMI 188

Query: 191 ALSEQELVDCD-RSYNEGCNGGLMDYAFEFIIDNGGLDTEEDYPYNGFDSSCIQYKKNAK 250
            LSEQELV+C     N GCNGGLMD AF+FII NGG+DTE+DYPY   D  C   ++NAK
Sbjct: 189 TLSEQELVECSTNGQNSGCNGGLMDDAFDFIIKNGGIDTEDDYPYKAVDGKCDINRENAK 248

Query: 251 VVAIDSYEDVPVNNEKALQKAVSKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNV 310
           VV+ID +EDVP N+EK+LQKAV+ Q VSVAIE GGR FQLY SG+F+GRCGT LDHGV  
Sbjct: 249 VVSIDGFEDVPQNDEKSLQKAVAHQPVSVAIEAGGREFQLYHSGVFSGRCGTSLDHGVVA 308

Query: 311 VGYGSKGGVDYWIVRNSWGGSWGESGYVRMQRNIASPTGLCGIAMEPSYPTKTGPNPPNP 370
           VGYG+  G DYWIVRNSWG  WGESGYVRM+RNI   TG CGIAM  SYPTK+G NPP P
Sbjct: 309 VGYGTDNGKDYWIVRNSWGPKWGESGYVRMERNINVTTGKCGIAMMASYPTKSGANPPKP 368

Query: 371 GPTPPSPVKPP------SVCDEYYTCPAAETCCCIFQFSNLCLEWGCCPLESATCCDDHY 430
            PTPP+P  PP       VCD+ ++CPA  TCCC F F NLCL WGCCP+E ATCC DH 
Sbjct: 369 SPTPPTPPTPPPPSAPDHVCDDNFSCPAGSTCCCAFGFRNLCLVWGCCPVEGATCCKDHA 428

Query: 431 SCCPHDYPICNVRAGTCSKSKNDIFGVKAMRRTAA 442
           SCCP DYP+CN RAGTCS SKN    VKA++RT A
Sbjct: 429 SCCPPDYPVCNTRAGTCSASKNSPLSVKALKRTLA 461

[Close]

BLAST of Chy7G129860 vs. ExPASy Swiss-Prot
Match: P20721 (Low-temperature-induced cysteine proteinase (Fragment) OS=Solanum lycopersicum OX=4081 PE=2 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 3.9e-145
Identity = 234/325 (72.00%), Postives = 274/325 (84.31%), Query Frame = 0


Query: 117 SNRYLPRLGDDLPDSIDWRAKGAVVAVKDQGSCGSCWAFSTVASVEAINQIVTGDLVALS 176
           S+RYLP++GD LP+SIDWR KG +V VKDQGSCGSCWAFS VA++E+IN IVTG+L++LS
Sbjct: 7   SDRYLPKVGDSLPESIDWREKGVLVGVKDQGSCGSCWAFSAVAAMESINAIVTGNLISLS 66

Query: 177 EQELVDCDRSYNEGCNGGLMDYAFEFIIDNGGLDTEEDYPYNGFDSSCIQYKKNAKVVAI 236
           EQELVDCDRSYNEGC+GGLMDYAFEF+I NGG+DTEEDYPY   +  C QY+KNAKVV I
Sbjct: 67  EQELVDCDRSYNEGCDGGLMDYAFEFVIKNGGIDTEEDYPYKERNGVCDQYRKNAKVVKI 126

Query: 237 DSYEDVPVNNEKALQKAVSKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYG 296
           DSYEDVPVNNEKALQKAV+ Q VS+A+E GGR FQ Y+SGIFTG+CGT +DHGV + GYG
Sbjct: 127 DSYEDVPVNNEKALQKAVAHQPVSIALEAGGRDFQHYKSGIFTGKCGTAVDHGVVIAGYG 186

Query: 297 SKGGVDYWIVRNSWGGSWGESGYVRMQRNIASPTGLCGIAMEPSYPTKTGPNPPNPGPTP 356
           ++ G+DYWIVRNSWG +  E+GY+R+QRN++S +GLCG+A+EPSYP KTGPNPP P P+P
Sbjct: 187 TENGMDYWIVRNSWGANCRENGYLRVQRNVSSSSGLCGLAIEPSYPVKTGPNPPKPAPSP 246

Query: 357 PSPVKPPSVCDEYYTCPAAETCCCIFQFSNLCLEWGCCPLESATCCDDHYSCCPHDYPIC 416
           PSPVKPP+ CDEY  C    TCCCI QF   C  WGCCPLE ATCC+DHYSCCPHDYPIC
Sbjct: 247 PSPVKPPTECDEYSQCAVGTTCCCILQFRRSCFSWGCCPLEGATCCEDHYSCCPHDYPIC 306

Query: 417 NVRAGTCSKSKNDIFGVKAMRRTAA 442
           NVR GTCS SK +  GVKAM+R  A
Sbjct: 307 NVRQGTCSMSKGNPLGVKAMKRILA 331

[Close]

BLAST of Chy7G129860 vs. ExPASy TrEMBL
Match: A0A0A0L3N7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G641600 PE=3 SV=1)

HSP 1 Score: 928.7 bits (2399), Expect = 9.4e-267
Identity = 446/459 (97.17%), Postives = 453/459 (98.69%), Query Frame = 0


Query: 1   MGTFQSSPIIALLFFLFIALSTASPSSIIPHRTDDEVMALYDQWRAKHGKLHNNLGAEPE 60
           MGTFQSSPI+ALLFFLFIALS ASPSSIIP RTDDEVMALYDQWRAKHGKLHNNLGAEPE
Sbjct: 1   MGTFQSSPIMALLFFLFIALSAASPSSIIPQRTDDEVMALYDQWRAKHGKLHNNLGAEPE 60

Query: 61  DRFHIFKDNLKFIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTSNRY 120
           +RFHIFKDNLKFIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTSNRY
Sbjct: 61  NRFHIFKDNLKFIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTSNRY 120

Query: 121 LPRLGDDLPDSIDWRAKGAVVAVKDQGSCGSCWAFSTVASVEAINQIVTGDLVALSEQEL 180
           LPRLGDDLPDSIDWRAKGAV  VKDQGSCGSCWAFSTVASVEAINQIVTGDL+ALSEQEL
Sbjct: 121 LPRLGDDLPDSIDWRAKGAVAPVKDQGSCGSCWAFSTVASVEAINQIVTGDLIALSEQEL 180

Query: 181 VDCDRSYNEGCNGGLMDYAFEFIIDNGGLDTEEDYPYNGFDSSCIQYKKNAKVVAIDSYE 240
           VDCDRSYNEGCNGGLMDYAFEFII+NGGLDTEEDYPY GFDSSCIQYKKNAKVVAID YE
Sbjct: 181 VDCDRSYNEGCNGGLMDYAFEFIIENGGLDTEEDYPYYGFDSSCIQYKKNAKVVAIDGYE 240

Query: 241 DVPVNNEKALQKAVSKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGSKGG 300
           DVPVNNEKALQKAVSKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGS+GG
Sbjct: 241 DVPVNNEKALQKAVSKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGSEGG 300

Query: 301 VDYWIVRNSWGGSWGESGYVRMQRNIASPTGLCGIAMEPSYPTKTGPNPPNPGPTPPSPV 360
           VDYWIVRNSWGGSWGESGYV+MQRNIASPTGLCGIAMEPSYPTKTGPNPPNPGPTPPSPV
Sbjct: 301 VDYWIVRNSWGGSWGESGYVKMQRNIASPTGLCGIAMEPSYPTKTGPNPPNPGPTPPSPV 360

Query: 361 KPPSVCDEYYTCPAAETCCCIFQFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRA 420
           KPPSVCDEYYTCPAAETCCCIFQFSNLCLEWGCCPLESATCCDDHYSCCPHDYP+CNVRA
Sbjct: 361 KPPSVCDEYYTCPAAETCCCIFQFSNLCLEWGCCPLESATCCDDHYSCCPHDYPVCNVRA 420

Query: 421 GTCSKSKNDIFGVKAMRRTAAAARPSWARRDVTVGKSSA 460
           GTCSKSKNDIFGVKAMRRTAAAARPSWARRDVTVGKSSA
Sbjct: 421 GTCSKSKNDIFGVKAMRRTAAAARPSWARRDVTVGKSSA 459

[Close]

BLAST of Chy7G129860 vs. ExPASy TrEMBL
Match: A0A1S3BV04 (low-temperature-induced cysteine proteinase-like OS=Cucumis melo OX=3656 GN=LOC103493570 PE=3 SV=1)

HSP 1 Score: 901.4 bits (2328), Expect = 1.6e-258
Identity = 430/459 (93.68%), Postives = 446/459 (97.17%), Query Frame = 0


Query: 1   MGTFQSSPIIALLFFLFIALSTASPSSIIPHRTDDEVMALYDQWRAKHGKLHNNLGAEPE 60
           MGTFQSS  +ALLFFLFIALSTASPSSIIP RTD+EVMALYDQWRA HGKLHNNLGAEPE
Sbjct: 1   MGTFQSSQTMALLFFLFIALSTASPSSIIPQRTDEEVMALYDQWRANHGKLHNNLGAEPE 60

Query: 61  DRFHIFKDNLKFIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTSNRY 120
           +RFHIFKDNLK+IDEINAQNLPYRLGLNVFADLTNEEYRSRYLGG+FA+GSRRNRTSNRY
Sbjct: 61  NRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGRFAAGSRRNRTSNRY 120

Query: 121 LPRLGDDLPDSIDWRAKGAVVAVKDQGSCGSCWAFSTVASVEAINQIVTGDLVALSEQEL 180
           LPRLGD+LPDSIDWRAKGAV  VKDQGSCGSCWAFSTVASVE INQIVTGDL+ALSEQEL
Sbjct: 121 LPRLGDELPDSIDWRAKGAVAPVKDQGSCGSCWAFSTVASVEGINQIVTGDLIALSEQEL 180

Query: 181 VDCDRSYNEGCNGGLMDYAFEFIIDNGGLDTEEDYPYNGFDSSCIQYKKNAKVVAIDSYE 240
           VDCD+SYNEGCNGGLMDYAFEFII+NGGLDTEEDYPY GFDSSCIQYKKNA+VV+ID YE
Sbjct: 181 VDCDKSYNEGCNGGLMDYAFEFIINNGGLDTEEDYPYYGFDSSCIQYKKNARVVSIDGYE 240

Query: 241 DVPVNNEKALQKAVSKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGSKGG 300
           DVPVNNEKALQKA SKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGS+GG
Sbjct: 241 DVPVNNEKALQKAASKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGSEGG 300

Query: 301 VDYWIVRNSWGGSWGESGYVRMQRNIASPTGLCGIAMEPSYPTKTGPNPPNPGPTPPSPV 360
           VDYWIVRNSWG +WGESGYVRMQRNI SPTGLCGIAMEPSYPTK+GPNPPNPGPTPPSPV
Sbjct: 301 VDYWIVRNSWGANWGESGYVRMQRNIDSPTGLCGIAMEPSYPTKSGPNPPNPGPTPPSPV 360

Query: 361 KPPSVCDEYYTCPAAETCCCIFQFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRA 420
           KPPSVCDEYYTCPAAETCCCIF+FSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRA
Sbjct: 361 KPPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRA 420

Query: 421 GTCSKSKNDIFGVKAMRRTAAAARPSWARRDVTVGKSSA 460
           GTCSKSKNDIFGVKAMRRTAAAARPSWA RDVTVGKSSA
Sbjct: 421 GTCSKSKNDIFGVKAMRRTAAAARPSWAHRDVTVGKSSA 459

[Close]

BLAST of Chy7G129860 vs. ExPASy TrEMBL
Match: A0A5A7VFD6 (Low-temperature-induced cysteine proteinase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003080 PE=3 SV=1)

HSP 1 Score: 889.0 bits (2296), Expect = 8.2e-255
Identity = 423/450 (94.00%), Postives = 439/450 (97.56%), Query Frame = 0


Query: 10  IALLFFLFIALSTASPSSIIPHRTDDEVMALYDQWRAKHGKLHNNLGAEPEDRFHIFKDN 69
           +ALLFFLFIALSTASPSSIIP RTD+EVMALYDQWRA HGKLHNNLGAEPE+RFHIFKDN
Sbjct: 1   MALLFFLFIALSTASPSSIIPQRTDEEVMALYDQWRANHGKLHNNLGAEPENRFHIFKDN 60

Query: 70  LKFIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTSNRYLPRLGDDLP 129
           LK+IDEINAQNLPYRLGLNVFADLTNEEYRSRYLGG+FA+GSRRNRTSNRYLPRLGD+LP
Sbjct: 61  LKYIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGRFAAGSRRNRTSNRYLPRLGDELP 120

Query: 130 DSIDWRAKGAVVAVKDQGSCGSCWAFSTVASVEAINQIVTGDLVALSEQELVDCDRSYNE 189
           DSIDWRAKGAV  VKDQGSCGSCWAFSTVASVE INQIVTGDL+ALSEQELVDCD+SYNE
Sbjct: 121 DSIDWRAKGAVAPVKDQGSCGSCWAFSTVASVEGINQIVTGDLIALSEQELVDCDKSYNE 180

Query: 190 GCNGGLMDYAFEFIIDNGGLDTEEDYPYNGFDSSCIQYKKNAKVVAIDSYEDVPVNNEKA 249
           GCNGGLMDYAFEFII+NGGLDTEEDYPY GFDSSCIQYKKNA+VV+ID YEDVPVNNEKA
Sbjct: 181 GCNGGLMDYAFEFIINNGGLDTEEDYPYYGFDSSCIQYKKNARVVSIDGYEDVPVNNEKA 240

Query: 250 LQKAVSKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGSKGGVDYWIVRNS 309
           LQKA SKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGS+GGVDYWIVRNS
Sbjct: 241 LQKAASKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGSEGGVDYWIVRNS 300

Query: 310 WGGSWGESGYVRMQRNIASPTGLCGIAMEPSYPTKTGPNPPNPGPTPPSPVKPPSVCDEY 369
           WG +WGESGYVRMQRNI SPTGLCGIAMEPSYPTK+GPNPPNPGPTPPSPVKPPSVCDEY
Sbjct: 301 WGANWGESGYVRMQRNIDSPTGLCGIAMEPSYPTKSGPNPPNPGPTPPSPVKPPSVCDEY 360

Query: 370 YTCPAAETCCCIFQFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRAGTCSKSKND 429
           YTCPAAETCCCIF+FSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRAGTCSKSKND
Sbjct: 361 YTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRAGTCSKSKND 420

Query: 430 IFGVKAMRRTAAAARPSWARRDVTVGKSSA 460
           IFGVKAMRRTAAAARPSWA RDVTVGKSSA
Sbjct: 421 IFGVKAMRRTAAAARPSWAHRDVTVGKSSA 450

[Close]

BLAST of Chy7G129860 vs. ExPASy TrEMBL
Match: A0A6J1FLR7 (low-temperature-induced cysteine proteinase-like OS=Cucurbita moschata OX=3662 GN=LOC111446375 PE=3 SV=1)

HSP 1 Score: 823.9 bits (2127), Expect = 3.3e-235
Identity = 391/461 (84.82%), Postives = 424/461 (91.97%), Query Frame = 0


Query: 1   MGTFQSSPIIALLFFLFIALST--ASPSSIIPHRTDDEVMALYDQWRAKHGKLHNNLGAE 60
           MGT++SS I+ LLFF+ I LS+  A  SSII  RTD+EVMALYDQWRAKHGKL N L  +
Sbjct: 1   MGTYRSSQIMPLLFFVIITLSSALALASSIISERTDEEVMALYDQWRAKHGKLRNGLRGQ 60

Query: 61  PEDRFHIFKDNLKFIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTSN 120
            +DRF IFKDNLK+IDEINAQNLPYRLGLNVFADLTN EYRS+YLG +FA+GSRRN+TS+
Sbjct: 61  RDDRFEIFKDNLKYIDEINAQNLPYRLGLNVFADLTNAEYRSKYLGTRFAAGSRRNKTSS 120

Query: 121 RYLPRLGDDLPDSIDWRAKGAVVAVKDQGSCGSCWAFSTVASVEAINQIVTGDLVALSEQ 180
           RY PRLGD LPDS+DWR KGAVV VKDQGSCGSCWAFST+A+VE INQ+VTGDLV+LSEQ
Sbjct: 121 RYQPRLGDKLPDSVDWREKGAVVPVKDQGSCGSCWAFSTIAAVEGINQVVTGDLVSLSEQ 180

Query: 181 ELVDCDRSYNEGCNGGLMDYAFEFIIDNGGLDTEEDYPYNGFDSSCIQYKKNAKVVAIDS 240
           ELVDCD SYNEGCNGGLMDYAFEFII+NGG+DTEEDYPYNGFDSSCIQYKKNA+VV+ID 
Sbjct: 181 ELVDCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSIDG 240

Query: 241 YEDVPVNNEKALQKAVSKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGSK 300
           YEDVPVN+EKALQKA + QVV+VAIEGGGRSFQLYQSGIFTG+CGT+LDHGVNVVGYGS+
Sbjct: 241 YEDVPVNDEKALQKAAANQVVAVAIEGGGRSFQLYQSGIFTGKCGTNLDHGVNVVGYGSE 300

Query: 301 GGVDYWIVRNSWGGSWGESGYVRMQRNIASPTGLCGIAMEPSYPTKTGPNPPNPGPTPPS 360
           GGVDYWIVRNSWGGSWGE GY+RMQRNI S TGLCGIAMEPSYPTK GPNPPNPGPTPPS
Sbjct: 301 GGVDYWIVRNSWGGSWGEHGYIRMQRNIDSKTGLCGIAMEPSYPTKNGPNPPNPGPTPPS 360

Query: 361 PVKPPSVCDEYYTCPAAETCCCIFQFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNV 420
           PVKPPSVCDEYYTCPAAETCCCIF+FSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNV
Sbjct: 361 PVKPPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNV 420

Query: 421 RAGTCSKSKNDIFGVKAMRRTAAAARPSWARRDVTVGKSSA 460
           RAGTC K KND+FGVKAMRRT AAA+PSWA  DV VGKSSA
Sbjct: 421 RAGTCLKGKNDVFGVKAMRRTTAAAKPSWAFGDVAVGKSSA 461

[Close]

BLAST of Chy7G129860 vs. ExPASy TrEMBL
Match: A0A6J1J4D3 (low-temperature-induced cysteine proteinase-like OS=Cucurbita maxima OX=3661 GN=LOC111481130 PE=3 SV=1)

HSP 1 Score: 822.4 bits (2123), Expect = 9.5e-235
Identity = 390/461 (84.60%), Postives = 425/461 (92.19%), Query Frame = 0


Query: 1   MGTFQSSPIIALLFFLFIALST--ASPSSIIPHRTDDEVMALYDQWRAKHGKLHNNLGAE 60
           MGT++SS I+ LLFF+ I LS+  A  SSII  RTD+EVMALYDQWRAKHGKL N L  +
Sbjct: 1   MGTYRSSQIMPLLFFVIITLSSALALASSIISERTDEEVMALYDQWRAKHGKLRNGLRGQ 60

Query: 61  PEDRFHIFKDNLKFIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTSN 120
            +DRF IFKDNLK+IDEINAQNLPYRLGLNVFADLTN EYRS+YLG +FA+GSRRN+TS+
Sbjct: 61  RDDRFEIFKDNLKYIDEINAQNLPYRLGLNVFADLTNAEYRSKYLGTRFAAGSRRNKTSS 120

Query: 121 RYLPRLGDDLPDSIDWRAKGAVVAVKDQGSCGSCWAFSTVASVEAINQIVTGDLVALSEQ 180
           RY PRLGD LP+S+DWR KGAVV VKDQGSCGSCWAFST+A+VE INQ+VTGDLV+LSEQ
Sbjct: 121 RYQPRLGDKLPESVDWREKGAVVPVKDQGSCGSCWAFSTIAAVEGINQVVTGDLVSLSEQ 180

Query: 181 ELVDCDRSYNEGCNGGLMDYAFEFIIDNGGLDTEEDYPYNGFDSSCIQYKKNAKVVAIDS 240
           ELVDCD SYNEGCNGGLMDYAFEFII+NGG+DTEEDYPYNGFDSSCIQYKKNA+VV+ID 
Sbjct: 181 ELVDCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSIDG 240

Query: 241 YEDVPVNNEKALQKAVSKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGSK 300
           YEDVPVN+EKALQKA + QVV+VAIEGGGRSFQLYQSGIFTG+CGT+LDHGVNVVGYGS+
Sbjct: 241 YEDVPVNDEKALQKAAANQVVAVAIEGGGRSFQLYQSGIFTGKCGTNLDHGVNVVGYGSE 300

Query: 301 GGVDYWIVRNSWGGSWGESGYVRMQRNIASPTGLCGIAMEPSYPTKTGPNPPNPGPTPPS 360
           GGVDYWIVRNSWGGSWGE GY+RMQRNIAS TGLCGIAMEPSYP K GPNPPNPGPTPPS
Sbjct: 301 GGVDYWIVRNSWGGSWGEHGYIRMQRNIASSTGLCGIAMEPSYPIKKGPNPPNPGPTPPS 360

Query: 361 PVKPPSVCDEYYTCPAAETCCCIFQFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNV 420
           PVKPPSVCDEYYTCPAAETCCCIF+FSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNV
Sbjct: 361 PVKPPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNV 420

Query: 421 RAGTCSKSKNDIFGVKAMRRTAAAARPSWARRDVTVGKSSA 460
           RAGTC K KND+FGVKAMRRT AAA+PSWA  DV+VGKSSA
Sbjct: 421 RAGTCLKGKNDVFGVKAMRRTTAAAKPSWAFGDVSVGKSSA 461

[Close]

BLAST of Chy7G129860 vs. NCBI nr
Match: XP_004141271.2 (low-temperature-induced cysteine proteinase [Cucumis sativus] >KGN55227.1 hypothetical protein Csa_012572 [Cucumis sativus])

HSP 1 Score: 926 bits (2392), Expect = 0.0
Identity = 446/459 (97.17%), Postives = 453/459 (98.69%), Query Frame = 0


Query: 1   MGTFQSSPIIALLFFLFIALSTASPSSIIPHRTDDEVMALYDQWRAKHGKLHNNLGAEPE 60
           MGTFQSSPI+ALLFFLFIALS ASPSSIIP RTDDEVMALYDQWRAKHGKLHNNLGAEPE
Sbjct: 1   MGTFQSSPIMALLFFLFIALSAASPSSIIPQRTDDEVMALYDQWRAKHGKLHNNLGAEPE 60

Query: 61  DRFHIFKDNLKFIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTSNRY 120
           +RFHIFKDNLKFIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTSNRY
Sbjct: 61  NRFHIFKDNLKFIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTSNRY 120

Query: 121 LPRLGDDLPDSIDWRAKGAVVAVKDQGSCGSCWAFSTVASVEAINQIVTGDLVALSEQEL 180
           LPRLGDDLPDSIDWRAKGAV  VKDQGSCGSCWAFSTVASVEAINQIVTGDL+ALSEQEL
Sbjct: 121 LPRLGDDLPDSIDWRAKGAVAPVKDQGSCGSCWAFSTVASVEAINQIVTGDLIALSEQEL 180

Query: 181 VDCDRSYNEGCNGGLMDYAFEFIIDNGGLDTEEDYPYNGFDSSCIQYKKNAKVVAIDSYE 240
           VDCDRSYNEGCNGGLMDYAFEFII+NGGLDTEEDYPY GFDSSCIQYKKNAKVVAID YE
Sbjct: 181 VDCDRSYNEGCNGGLMDYAFEFIIENGGLDTEEDYPYYGFDSSCIQYKKNAKVVAIDGYE 240

Query: 241 DVPVNNEKALQKAVSKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGSKGG 300
           DVPVNNEKALQKAVSKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGS+GG
Sbjct: 241 DVPVNNEKALQKAVSKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGSEGG 300

Query: 301 VDYWIVRNSWGGSWGESGYVRMQRNIASPTGLCGIAMEPSYPTKTGPNPPNPGPTPPSPV 360
           VDYWIVRNSWGGSWGESGYV+MQRNIASPTGLCGIAMEPSYPTKTGPNPPNPGPTPPSPV
Sbjct: 301 VDYWIVRNSWGGSWGESGYVKMQRNIASPTGLCGIAMEPSYPTKTGPNPPNPGPTPPSPV 360

Query: 361 KPPSVCDEYYTCPAAETCCCIFQFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRA 420
           KPPSVCDEYYTCPAAETCCCIFQFSNLCLEWGCCPLESATCCDDHYSCCPHDYP+CNVRA
Sbjct: 361 KPPSVCDEYYTCPAAETCCCIFQFSNLCLEWGCCPLESATCCDDHYSCCPHDYPVCNVRA 420

Query: 421 GTCSKSKNDIFGVKAMRRTAAAARPSWARRDVTVGKSSA 459
           GTCSKSKNDIFGVKAMRRTAAAARPSWARRDVTVGKSSA
Sbjct: 421 GTCSKSKNDIFGVKAMRRTAAAARPSWARRDVTVGKSSA 459

[Close]

BLAST of Chy7G129860 vs. NCBI nr
Match: XP_008452589.2 (PREDICTED: low-temperature-induced cysteine proteinase-like [Cucumis melo])

HSP 1 Score: 898 bits (2321), Expect = 0.0
Identity = 430/459 (93.68%), Postives = 446/459 (97.17%), Query Frame = 0


Query: 1   MGTFQSSPIIALLFFLFIALSTASPSSIIPHRTDDEVMALYDQWRAKHGKLHNNLGAEPE 60
           MGTFQSS  +ALLFFLFIALSTASPSSIIP RTD+EVMALYDQWRA HGKLHNNLGAEPE
Sbjct: 1   MGTFQSSQTMALLFFLFIALSTASPSSIIPQRTDEEVMALYDQWRANHGKLHNNLGAEPE 60

Query: 61  DRFHIFKDNLKFIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTSNRY 120
           +RFHIFKDNLK+IDEINAQNLPYRLGLNVFADLTNEEYRSRYLGG+FA+GSRRNRTSNRY
Sbjct: 61  NRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGRFAAGSRRNRTSNRY 120

Query: 121 LPRLGDDLPDSIDWRAKGAVVAVKDQGSCGSCWAFSTVASVEAINQIVTGDLVALSEQEL 180
           LPRLGD+LPDSIDWRAKGAV  VKDQGSCGSCWAFSTVASVE INQIVTGDL+ALSEQEL
Sbjct: 121 LPRLGDELPDSIDWRAKGAVAPVKDQGSCGSCWAFSTVASVEGINQIVTGDLIALSEQEL 180

Query: 181 VDCDRSYNEGCNGGLMDYAFEFIIDNGGLDTEEDYPYNGFDSSCIQYKKNAKVVAIDSYE 240
           VDCD+SYNEGCNGGLMDYAFEFII+NGGLDTEEDYPY GFDSSCIQYKKNA+VV+ID YE
Sbjct: 181 VDCDKSYNEGCNGGLMDYAFEFIINNGGLDTEEDYPYYGFDSSCIQYKKNARVVSIDGYE 240

Query: 241 DVPVNNEKALQKAVSKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGSKGG 300
           DVPVNNEKALQKA SKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGS+GG
Sbjct: 241 DVPVNNEKALQKAASKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGSEGG 300

Query: 301 VDYWIVRNSWGGSWGESGYVRMQRNIASPTGLCGIAMEPSYPTKTGPNPPNPGPTPPSPV 360
           VDYWIVRNSWG +WGESGYVRMQRNI SPTGLCGIAMEPSYPTK+GPNPPNPGPTPPSPV
Sbjct: 301 VDYWIVRNSWGANWGESGYVRMQRNIDSPTGLCGIAMEPSYPTKSGPNPPNPGPTPPSPV 360

Query: 361 KPPSVCDEYYTCPAAETCCCIFQFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRA 420
           KPPSVCDEYYTCPAAETCCCIF+FSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRA
Sbjct: 361 KPPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRA 420

Query: 421 GTCSKSKNDIFGVKAMRRTAAAARPSWARRDVTVGKSSA 459
           GTCSKSKNDIFGVKAMRRTAAAARPSWA RDVTVGKSSA
Sbjct: 421 GTCSKSKNDIFGVKAMRRTAAAARPSWAHRDVTVGKSSA 459

[Close]

BLAST of Chy7G129860 vs. NCBI nr
Match: KAA0064411.1 (low-temperature-induced cysteine proteinase-like [Cucumis melo var. makuwa] >TYK20176.1 low-temperature-induced cysteine proteinase-like [Cucumis melo var. makuwa])

HSP 1 Score: 886 bits (2289), Expect = 0.0
Identity = 423/449 (94.21%), Postives = 438/449 (97.55%), Query Frame = 0


Query: 11  ALLFFLFIALSTASPSSIIPHRTDDEVMALYDQWRAKHGKLHNNLGAEPEDRFHIFKDNL 70
           ALLFFLFIALSTASPSSIIP RTD+EVMALYDQWRA HGKLHNNLGAEPE+RFHIFKDNL
Sbjct: 2   ALLFFLFIALSTASPSSIIPQRTDEEVMALYDQWRANHGKLHNNLGAEPENRFHIFKDNL 61

Query: 71  KFIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTSNRYLPRLGDDLPD 130
           K+IDEINAQNLPYRLGLNVFADLTNEEYRSRYLGG+FA+GSRRNRTSNRYLPRLGD+LPD
Sbjct: 62  KYIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGRFAAGSRRNRTSNRYLPRLGDELPD 121

Query: 131 SIDWRAKGAVVAVKDQGSCGSCWAFSTVASVEAINQIVTGDLVALSEQELVDCDRSYNEG 190
           SIDWRAKGAV  VKDQGSCGSCWAFSTVASVE INQIVTGDL+ALSEQELVDCD+SYNEG
Sbjct: 122 SIDWRAKGAVAPVKDQGSCGSCWAFSTVASVEGINQIVTGDLIALSEQELVDCDKSYNEG 181

Query: 191 CNGGLMDYAFEFIIDNGGLDTEEDYPYNGFDSSCIQYKKNAKVVAIDSYEDVPVNNEKAL 250
           CNGGLMDYAFEFII+NGGLDTEEDYPY GFDSSCIQYKKNA+VV+ID YEDVPVNNEKAL
Sbjct: 182 CNGGLMDYAFEFIINNGGLDTEEDYPYYGFDSSCIQYKKNARVVSIDGYEDVPVNNEKAL 241

Query: 251 QKAVSKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGSKGGVDYWIVRNSW 310
           QKA SKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGS+GGVDYWIVRNSW
Sbjct: 242 QKAASKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGSEGGVDYWIVRNSW 301

Query: 311 GGSWGESGYVRMQRNIASPTGLCGIAMEPSYPTKTGPNPPNPGPTPPSPVKPPSVCDEYY 370
           G +WGESGYVRMQRNI SPTGLCGIAMEPSYPTK+GPNPPNPGPTPPSPVKPPSVCDEYY
Sbjct: 302 GANWGESGYVRMQRNIDSPTGLCGIAMEPSYPTKSGPNPPNPGPTPPSPVKPPSVCDEYY 361

Query: 371 TCPAAETCCCIFQFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRAGTCSKSKNDI 430
           TCPAAETCCCIF+FSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRAGTCSKSKNDI
Sbjct: 362 TCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRAGTCSKSKNDI 421

Query: 431 FGVKAMRRTAAAARPSWARRDVTVGKSSA 459
           FGVKAMRRTAAAARPSWA RDVTVGKSSA
Sbjct: 422 FGVKAMRRTAAAARPSWAHRDVTVGKSSA 450

[Close]

BLAST of Chy7G129860 vs. NCBI nr
Match: XP_038897503.1 (low-temperature-induced cysteine proteinase-like [Benincasa hispida])

HSP 1 Score: 880 bits (2275), Expect = 0.0
Identity = 415/459 (90.41%), Postives = 442/459 (96.30%), Query Frame = 0


Query: 1   MGTFQSSPIIALLFFLFIALSTASPSSIIPHRTDDEVMALYDQWRAKHGKLHNNLGAEPE 60
           MGT+ SSPI+ALLFFLFIALS+ASPSSIIP RTD+EVMALYDQWRAKHGKL NNLGAEPE
Sbjct: 1   MGTYLSSPIMALLFFLFIALSSASPSSIIPERTDEEVMALYDQWRAKHGKLRNNLGAEPE 60

Query: 61  DRFHIFKDNLKFIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTSNRY 120
           +RFHIFKDNLK+IDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFA+GSRRNRTS+RY
Sbjct: 61  NRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFAAGSRRNRTSSRY 120

Query: 121 LPRLGDDLPDSIDWRAKGAVVAVKDQGSCGSCWAFSTVASVEAINQIVTGDLVALSEQEL 180
           LPRLGD++PDSIDWR KGAV  VKDQGSCGSCWAFST+A+VE INQIVTGDL+ALSEQEL
Sbjct: 121 LPRLGDEIPDSIDWREKGAVAPVKDQGSCGSCWAFSTIAAVEGINQIVTGDLIALSEQEL 180

Query: 181 VDCDRSYNEGCNGGLMDYAFEFIIDNGGLDTEEDYPYNGFDSSCIQYKKNAKVVAIDSYE 240
           VDCD+SYNEGCNGGLMDYAFEFII+NGG+DTEEDYPYNGFDSSCIQYKKNA+VV+ID YE
Sbjct: 181 VDCDKSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSIDGYE 240

Query: 241 DVPVNNEKALQKAVSKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGSKGG 300
           DVP+NNEKALQKA SKQVV+VAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGS+GG
Sbjct: 241 DVPINNEKALQKAASKQVVAVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGSEGG 300

Query: 301 VDYWIVRNSWGGSWGESGYVRMQRNIASPTGLCGIAMEPSYPTKTGPNPPNPGPTPPSPV 360
           VDYWIVRNSWG SWGESGY+RMQRNIAS TGLCGIAMEPSYPTK+GPNPPNPGPTPPSPV
Sbjct: 301 VDYWIVRNSWGASWGESGYIRMQRNIASSTGLCGIAMEPSYPTKSGPNPPNPGPTPPSPV 360

Query: 361 KPPSVCDEYYTCPAAETCCCIFQFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRA 420
           KPPSVCDEYYTCPAAETCCC+F+FS +CLEWGCCPLESATCCDDHYSCCPHDYPICNVRA
Sbjct: 361 KPPSVCDEYYTCPAAETCCCVFEFSTMCLEWGCCPLESATCCDDHYSCCPHDYPICNVRA 420

Query: 421 GTCSKSKNDIFGVKAMRRTAAAARPSWARRDVTVGKSSA 459
           GTC K KND+FGVKAMRRT AAARPSWA  DVTVGKSSA
Sbjct: 421 GTCLKGKNDVFGVKAMRRTTAAARPSWAHGDVTVGKSSA 459

[Close]

BLAST of Chy7G129860 vs. NCBI nr
Match: KAG6608453.1 (Cysteine proteinase RD21A, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037789.1 Cysteine proteinase RD21A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 823 bits (2127), Expect = 2.30e-299
Identity = 392/461 (85.03%), Postives = 425/461 (92.19%), Query Frame = 0


Query: 1   MGTFQSSPIIALLFFLFIALSTASP--SSIIPHRTDDEVMALYDQWRAKHGKLHNNLGAE 60
           MGT++SS I+ LLFF+ I LS+A    SSII  RTD+EVMALYDQWRAKHGKL N L  +
Sbjct: 1   MGTYRSSQIMPLLFFVVITLSSALAFASSIISERTDEEVMALYDQWRAKHGKLRNGLRGQ 60

Query: 61  PEDRFHIFKDNLKFIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTSN 120
            +DRF IFKDNLK+IDEINAQNLPYRLGLNVFADLTN EYRS+YLG +FA+GSRRN+TS+
Sbjct: 61  RDDRFEIFKDNLKYIDEINAQNLPYRLGLNVFADLTNAEYRSKYLGTRFAAGSRRNKTSS 120

Query: 121 RYLPRLGDDLPDSIDWRAKGAVVAVKDQGSCGSCWAFSTVASVEAINQIVTGDLVALSEQ 180
           RY PRLGD LPDS+DWR KGAVV VKDQGSCGSCWAFST+A+VE INQ+VTGDLV+LSEQ
Sbjct: 121 RYQPRLGDKLPDSVDWREKGAVVPVKDQGSCGSCWAFSTIAAVEGINQVVTGDLVSLSEQ 180

Query: 181 ELVDCDRSYNEGCNGGLMDYAFEFIIDNGGLDTEEDYPYNGFDSSCIQYKKNAKVVAIDS 240
           ELVDCD SYNEGCNGGLMDYAFEFII+NGG+DTEEDYPYNGFDSSCIQYKKNA+VV+ID 
Sbjct: 181 ELVDCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSIDG 240

Query: 241 YEDVPVNNEKALQKAVSKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGSK 300
           YEDVPVN+EKALQKA + QVV+VAIEGGGRSFQLYQSGIFTG+CGT+LDHGVNVVGYGS+
Sbjct: 241 YEDVPVNDEKALQKAAANQVVAVAIEGGGRSFQLYQSGIFTGKCGTNLDHGVNVVGYGSE 300

Query: 301 GGVDYWIVRNSWGGSWGESGYVRMQRNIASPTGLCGIAMEPSYPTKTGPNPPNPGPTPPS 360
           GGVDYWIVRNSWGGSWGE GY+RMQRNI SPTGLCGIAMEPSYPTK GPNPPNPGPTPPS
Sbjct: 301 GGVDYWIVRNSWGGSWGEHGYIRMQRNIDSPTGLCGIAMEPSYPTKNGPNPPNPGPTPPS 360

Query: 361 PVKPPSVCDEYYTCPAAETCCCIFQFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNV 420
           PVKPPSVCDEYYTCPAAETCCCIF+FSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNV
Sbjct: 361 PVKPPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNV 420

Query: 421 RAGTCSKSKNDIFGVKAMRRTAAAARPSWARRDVTVGKSSA 459
           RAGTC K KND+FGVKAMRRT AAA+PSWA  DV VGKSSA
Sbjct: 421 RAGTCLKGKNDVFGVKAMRRTTAAAKPSWAFGDVAVGKSSA 461

[Close]

BLAST of Chy7G129860 vs. TAIR 10
Match: AT1G47128.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 610.5 bits (1573), Expect = 1.1e-174
Identity = 290/454 (63.88%), Postives = 349/454 (76.87%), Query Frame = 0


Query: 8   PIIALLFFLFIALSTASPSSIIPH-----------RTDDEVMALYDQWRAKHGKLHN-NL 67
           P +A+LF   +A+S+A   SII +           R++ EVM++Y+ W  KHGK  + N 
Sbjct: 6   PTMAILFLAMVAVSSAVDMSIISYDEKHGVSTTGGRSEAEVMSIYEAWLVKHGKAQSQNS 65

Query: 68  GAEPEDRFHIFKDNLKFIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNR 127
             E + RF IFKDNL+F+DE N +NL YRLGL  FADLTN+EYRS+YLG K      R R
Sbjct: 66  LVEKDRRFEIFKDNLRFVDEHNEKNLSYRLGLTRFADLTNDEYRSKYLGAKMEKKGER-R 125

Query: 128 TSNRYLPRLGDDLPDSIDWRAKGAVVAVKDQGSCGSCWAFSTVASVEAINQIVTGDLVAL 187
           TS RY  R+GD+LP+SIDWR KGAV  VKDQG CGSCWAFST+ +VE INQIVTGDL+ L
Sbjct: 126 TSLRYEARVGDELPESIDWRKKGAVAEVKDQGGCGSCWAFSTIGAVEGINQIVTGDLITL 185

Query: 188 SEQELVDCDRSYNEGCNGGLMDYAFEFIIDNGGLDTEEDYPYNGFDSSCIQYKKNAKVVA 247
           SEQELVDCD SYNEGCNGGLMDYAFEFII NGG+DT++DYPY G D +C Q +KNAKVV 
Sbjct: 186 SEQELVDCDTSYNEGCNGGLMDYAFEFIIKNGGIDTDKDYPYKGVDGTCDQIRKNAKVVT 245

Query: 248 IDSYEDVPVNNEKALQKAVSKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGY 307
           IDSYEDVP  +E++L+KAV+ Q +S+AIE GGR+FQLY SGIF G CGT LDHGV  VGY
Sbjct: 246 IDSYEDVPTYSEESLKKAVAHQPISIAIEAGGRAFQLYDSGIFDGSCGTQLDHGVVAVGY 305

Query: 308 GSKGGVDYWIVRNSWGGSWGESGYVRMQRNIASPTGLCGIAMEPSYPTKTGPNPPNPGPT 367
           G++ G DYWIVRNSWG SWGESGY+RM RNIAS +G CGIA+EPSYP K G NPPNPGP+
Sbjct: 306 GTENGKDYWIVRNSWGKSWGESGYLRMARNIASSSGKCGIAIEPSYPIKNGENPPNPGPS 365

Query: 368 PPSPVKPPSVCDEYYTCPAAETCCCIFQFSNLCLEWGCCPLESATCCDDHYSCCPHDYPI 427
           PPSP+KPP+ CD YYTCP + TCCC+F++   C  WGCCPLE+ATCCDD+YSCCPH+YP+
Sbjct: 366 PPSPIKPPTQCDSYYTCPESNTCCCLFEYGKYCFAWGCCPLEAATCCDDNYSCCPHEYPV 425

Query: 428 CNVRAGTCSKSKNDIFGVKAMRRTAAAARPSWAR 450
           C++  GTC  SKN  F VKA++R  A   P W++
Sbjct: 426 CDLDQGTCLLSKNSPFSVKALKRKPAT--PFWSQ 456

[Close]

BLAST of Chy7G129860 vs. TAIR 10
Match: AT5G43060.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 599.7 bits (1545), Expect = 1.9e-171
Identity = 290/463 (62.63%), Postives = 348/463 (75.16%), Query Frame = 0


Query: 1   MGTFQSSPIIALLFFLFIALSTASPSSIIPH-----------RTDDEVMALYDQWRAKHG 60
           MG  + SP+I LL    I +S A   SII +           R+D EV  +Y+ W  +HG
Sbjct: 1   MGFLKLSPMILLL--AMIGVSYAMDMSIISYDENHHITTETSRSDSEVERIYEAWMVEHG 60

Query: 61  KL---HNNLGAEPEDRFHIFKDNLKFIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGK 120
           K     N LGAE + RF IFKDNL+FIDE N +NL Y+LGL  FADLTNEEYRS YLG K
Sbjct: 61  KKKMNQNGLGAEKDQRFEIFKDNLRFIDEHNTKNLSYKLGLTRFADLTNEEYRSMYLGAK 120

Query: 121 FASGSRRNRTSNRYLPRLGDDLPDSIDWRAKGAVVAVKDQGSCGSCWAFSTVASVEAINQ 180
                R  +TS+RY  R+GD LPDS+DWR +GAV  VKDQGSCGSCWAFST+ +VE IN+
Sbjct: 121 --PTKRVLKTSDRYQARVGDALPDSVDWRKEGAVADVKDQGSCGSCWAFSTIGAVEGINK 180

Query: 181 IVTGDLVALSEQELVDCDRSYNEGCNGGLMDYAFEFIIDNGGLDTEEDYPYNGFDSSCIQ 240
           IVTGDL++LSEQELVDCD SYN+GCNGGLMDYAFEFII NGG+DTE DYPY   D  C Q
Sbjct: 181 IVTGDLISLSEQELVDCDTSYNQGCNGGLMDYAFEFIIKNGGIDTEADYPYKAADGRCDQ 240

Query: 241 YKKNAKVVAIDSYEDVPVNNEKALQKAVSKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDL 300
            +KNAKVV IDSYEDVP N+E +L+KA++ Q +SVAIE GGR+FQLY SG+F G CGT+L
Sbjct: 241 NRKNAKVVTIDSYEDVPENSEASLKKALAHQPISVAIEAGGRAFQLYSSGVFDGLCGTEL 300

Query: 301 DHGVNVVGYGSKGGVDYWIVRNSWGGSWGESGYVRMQRNIASPTGLCGIAMEPSYPTKTG 360
           DHGV  VGYG++ G DYWIVRNSWG  WGESGY++M RNI +PTG CGIAME SYP K G
Sbjct: 301 DHGVVAVGYGTENGKDYWIVRNSWGNRWGESGYIKMARNIEAPTGKCGIAMEASYPIKKG 360

Query: 361 PNPPNPGPTPPSPVKPPSVCDEYYTCPAAETCCCIFQFSNLCLEWGCCPLESATCCDDHY 420
            NPPNPGP+PPSP+KPP+ CD+Y++CP + TCCC++++   C  WGCCPLE+ATCCDD+ 
Sbjct: 361 QNPPNPGPSPPSPIKPPTTCDKYFSCPESNTCCCLYKYGKYCFGWGCCPLEAATCCDDNS 420

Query: 421 SCCPHDYPICNVRAGTCSKSKNDIFGVKAMRRTAAAARPSWAR 450
           SCCPH+YP+C+V  GTC  SKN  F VKA++RT A   P WA+
Sbjct: 421 SCCPHEYPVCDVNRGTCLMSKNSPFSVKALKRTPAI--PFWAK 457

[Close]

BLAST of Chy7G129860 vs. TAIR 10
Match: AT3G19390.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 506.9 bits (1304), Expect = 1.7e-143
Identity = 255/448 (56.92%), Postives = 318/448 (70.98%), Query Frame = 0


Query: 1   MGTFQSSPIIALLFF--LFIALSTASPSSIIPHRTDDEVMALYDQWRAKHGKLHNNLGAE 60
           M T   S  +ALL F  L I+LS  S ++    R + E   +Y++W  ++ K +N LG E
Sbjct: 1   MATSIKSITLALLIFSVLLISLSLGSVTATETTRNEAEARRMYERWLVENRKNYNGLG-E 60

Query: 61  PEDRFHIFKDNLKFIDEINA-QNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTS 120
            E RF IFKDNLKF++E ++  N  Y +GL  FADLTN+E+R+ YL  K    +R     
Sbjct: 61  KERRFEIFKDNLKFVEEHSSIPNRTYEVGLTRFADLTNDEFRAIYLRSKM-ERTRVPVKG 120

Query: 121 NRYLPRLGDDLPDSIDWRAKGAVVAVKDQGSCGSCWAFSTVASVEAINQIVTGDLVALSE 180
            +YL ++GD LPD+IDWRAKGAV  VKDQGSCGSCWAFS + +VE INQI TG+L++LSE
Sbjct: 121 EKYLYKVGDSLPDAIDWRAKGAVNPVKDQGSCGSCWAFSAIGAVEGINQIKTGELISLSE 180

Query: 181 QELVDCDRSYNEGCNGGLMDYAFEFIIDNGGLDTEEDYPYNGFD-SSCIQYKKNAKVVAI 240
           QELVDCD SYN+GC GGLMDYAF+FII+NGG+DTEEDYPY   D + C   KKN +VV I
Sbjct: 181 QELVDCDTSYNDGCGGGLMDYAFKFIIENGGIDTEEDYPYIATDVNVCNSDKKNTRVVTI 240

Query: 241 DSYEDVPVNNEKALQKAVSKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYG 300
           D YEDVP N+EK+L+KA++ Q +SVAIE GGR+FQLY SG+FTG CGT LDHGV  VGYG
Sbjct: 241 DGYEDVPQNDEKSLKKALANQPISVAIEAGGRAFQLYTSGVFTGTCGTSLDHGVVAVGYG 300

Query: 301 SKGGVDYWIVRNSWGGSWGESGYVRMQRNIASPTGLCGIAMEPSYPTK-TGPNPPNPGPT 360
           S+GG DYWIVRNSWG +WGESGY +++RNI   +G CG+AM  SYPTK +G NPP P   
Sbjct: 301 SEGGQDYWIVRNSWGSNWGESGYFKLERNIKESSGKCGVAMMASYPTKSSGSNPPKP--- 360

Query: 361 PPSPVKPPSVCDEYYTCPAAETCCCIFQFSNLCLEWGCCPLESATCCDDHYSCCPHDYPI 420
              P   P VCD+  TCPA  TCCC+++++  C  WGCCP ESATCCDD  SCCP  YP+
Sbjct: 361 ---PAPSPVVCDKSNTCPAKSTCCCLYEYNGKCYSWGCCPYESATCCDDGSSCCPQSYPV 420

Query: 421 CNVRAGTCSKSKNDIFGVKAMRRTAAAA 444
           C+++A TC    N    +KA+ R  A A
Sbjct: 421 CDLKANTCRMKGNSPLSIKALTRGPAIA 440

[Close]

BLAST of Chy7G129860 vs. TAIR 10
Match: AT1G09850.1 (xylem bark cysteine peptidase 3 )

HSP 1 Score: 430.6 bits (1106), Expect = 1.5e-120
Identity = 220/433 (50.81%), Postives = 279/433 (64.43%), Query Frame = 0


Query: 3   TFQSSPIIALLFFLFIALSTASPSSIIPHRTDDEVMALYDQWRAKHGKLHNNLGAEPEDR 62
           +  SS  I+L FF  + +S++S S        D++  L+D W  KHGK + +   E + R
Sbjct: 2   SMSSSSFISLTFFFLLLVSSSSSS--------DDISELFDDWCQKHGKTYGS-EEERQQR 61

Query: 63  FHIFKDNLKFIDEIN-AQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTSNRYL 122
             IFKDN  F+ + N   N  Y L LN FADLT+ E+++  LG   ++ S    +  + L
Sbjct: 62  IQIFKDNHDFVTQHNLITNATYSLSLNAFADLTHHEFKASRLGLSVSAPSVIMASKGQSL 121

Query: 123 PRLGDDLPDSIDWRAKGAVVAVKDQGSCGSCWAFSTVASVEAINQIVTGDLVALSEQELV 182
                 +PDS+DWR KGAV  VKDQGSCG+CW+FS   ++E INQIVTGDL++LSEQEL+
Sbjct: 122 GG-SVKVPDSVDWRKKGAVTNVKDQGSCGACWSFSATGAMEGINQIVTGDLISLSEQELI 181

Query: 183 DCDRSYNEGCNGGLMDYAFEFIIDNGGLDTEEDYPYNGFDSSCIQYKKNAKVVAIDSYED 242
           DCD+SYN GCNGGLMDYAFEF+I N G+DTE+DYPY   D +C + K   KVV IDSY  
Sbjct: 182 DCDKSYNAGCNGGLMDYAFEFVIKNHGIDTEKDYPYQERDGTCKKDKLKQKVVTIDSYAG 241

Query: 243 VPVNNEKALQKAVSKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGSKGGV 302
           V  N+EKAL +AV+ Q VSV I G  R+FQLY SGIF+G C T LDH V +VGYGS+ GV
Sbjct: 242 VKSNDEKALMEAVAAQPVSVGICGSERAFQLYSSGIFSGPCSTSLDHAVLIVGYGSQNGV 301

Query: 303 DYWIVRNSWGGSWGESGYVRMQRNIASPTGLCGIAMEPSYPTKTGPNPPNPGPTPPSPVK 362
           DYWIV+NSWG SWG  G++ MQRN  +  G+CGI M  SYP KT PNP  P P+PP P K
Sbjct: 302 DYWIVKNSWGKSWGMDGFMHMQRNTENSDGVCGINMLASYPIKTHPNP--PPPSPPGPTK 361

Query: 363 PPSVCDEYYTCPAAETCCCIFQFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRAG 422
               C+ +  C + ETCCC  +   LC  W CC +ESA CC D   CCPHDYP+C+    
Sbjct: 362 ----CNLFTYCSSGETCCCARELFGLCFSWKCCEIESAVCCKDGRHCCPHDYPVCDTTRS 418

Query: 423 TCSKSKNDIFGVK 435
            C K   +   +K
Sbjct: 422 LCLKKTGNFTAIK 418

[Close]

BLAST of Chy7G129860 vs. TAIR 10
Match: AT4G36880.1 (cysteine proteinase1 )

HSP 1 Score: 412.9 bits (1060), Expect = 3.3e-115
Identity = 207/357 (57.98%), Postives = 261/357 (73.11%), Query Frame = 0


Query: 12  LLFFLFIALSTASPSSIIPH---------RTDDEVMALYDQWRAKHGKLHNNLGA---EP 71
           LL ++ ++L++   S I  H         RTD+EV ++Y QW A+HGK +NN      + 
Sbjct: 11  LLLYVVVSLASGDESIINDHLQLPSDGKWRTDEEVRSIYLQWSAEHGKTNNNNNGIINDQ 70

Query: 72  EDRFHIFKDNLKFID--EINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTS 131
           + RF+IFKDNL+FID    N +N  Y+LGL  F DLTN+EYR  YLG +     R  +  
Sbjct: 71  DKRFNIFKDNLRFIDLHNENNKNATYKLGLTKFTDLTNDEYRKLYLGARTEPARRIAKAK 130

Query: 132 N---RYLPRL-GDDLPDSIDWRAKGAVVAVKDQGSCGSCWAFSTVASVEAINQIVTGDLV 191
           N   +Y   + G ++P+++DWR KGAV  +KDQG+CGSCWAFST A+VE IN+IVTG+L+
Sbjct: 131 NVNQKYSAAVNGKEVPETVDWRQKGAVNPIKDQGTCGSCWAFSTTAAVEGINKIVTGELI 190

Query: 192 ALSEQELVDCDRSYNEGCNGGLMDYAFEFIIDNGGLDTEEDYPYNGFDSSCIQYKKNAKV 251
           +LSEQELVDCD+SYN+GCNGGLMDYAF+FI+ NGGL+TE+DYPY GF   C  + KN++V
Sbjct: 191 SLSEQELVDCDKSYNQGCNGGLMDYAFQFIMKNGGLNTEKDYPYRGFGGKCNSFLKNSRV 250

Query: 252 VAIDSYEDVPVNNEKALQKAVSKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVV 311
           V+ID YEDVP  +E AL+KA+S Q VSVAIE GGR FQ YQSGIFTG CGT+LDH V  V
Sbjct: 251 VSIDGYEDVPTKDETALKKAISYQPVSVAIEAGGRIFQHYQSGIFTGSCGTNLDHAVVAV 310

Query: 312 GYGSKGGVDYWIVRNSWGGSWGESGYVRMQRNI-ASPTGLCGIAMEPSYPTKTGPNP 350
           GYGS+ GVDYWIVRNSWG  WGE GY+RM+RN+ AS +G CGIA+E SYP K  PNP
Sbjct: 311 GYGSENGVDYWIVRNSWGPRWGEEGYIRMERNLAASKSGKCGIAVEASYPVKYSPNP 367

The following BLAST results are available for this feature:

BLAST of Chy7G129860 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P43297	1.5e-173	63.88	Cysteine proteinase RD21A OS=Arabidopsis thaliana OX=3702 GN=RD21A PE=1 SV=1	[more]
Q9FMH8	2.7e-170	62.63	Probable cysteine protease RD21B OS=Arabidopsis thaliana OX=3702 GN=RD21B PE=1 S...	[more]
P25776	3.8e-164	63.27	Oryzain alpha chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0650000 PE=...	[more]
P25777	5.0e-148	58.90	Oryzain beta chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0670200 PE=1...	[more]
P20721	3.9e-145	72.00	Low-temperature-induced cysteine proteinase (Fragment) OS=Solanum lycopersicum O...	[more]

BLAST of Chy7G129860 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L3N7	9.4e-267	97.17	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G641600 PE=3 SV=1	[more]
A0A1S3BV04	1.6e-258	93.68	low-temperature-induced cysteine proteinase-like OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A5A7VFD6	8.2e-255	94.00	Low-temperature-induced cysteine proteinase-like OS=Cucumis melo var. makuwa OX=...	[more]
A0A6J1FLR7	3.3e-235	84.82	low-temperature-induced cysteine proteinase-like OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1J4D3	9.5e-235	84.60	low-temperature-induced cysteine proteinase-like OS=Cucurbita maxima OX=3661 GN=...	[more]

BLAST of Chy7G129860 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004141271.2	0.0	97.17	low-temperature-induced cysteine proteinase [Cucumis sativus] >KGN55227.1 hypoth...	[more]
XP_008452589.2	0.0	93.68	PREDICTED: low-temperature-induced cysteine proteinase-like [Cucumis melo]	[more]
KAA0064411.1	0.0	94.21	low-temperature-induced cysteine proteinase-like [Cucumis melo var. makuwa] >TYK...	[more]
XP_038897503.1	0.0	90.41	low-temperature-induced cysteine proteinase-like [Benincasa hispida]	[more]
KAG6608453.1	2.30e-299	85.03	Cysteine proteinase RD21A, partial [Cucurbita argyrosperma subsp. sororia] >KAG7...	[more]

BLAST of Chy7G129860 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G47128.1	1.1e-174	63.88	Granulin repeat cysteine protease family protein	[more]
AT5G43060.1	1.9e-171	62.63	Granulin repeat cysteine protease family protein	[more]
AT3G19390.1	1.7e-143	56.92	Granulin repeat cysteine protease family protein	[more]
AT1G09850.1	1.5e-120	50.81	xylem bark cysteine peptidase 3	[more]
AT4G36880.1	3.3e-115	57.98	cysteine proteinase1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000668	Peptidase C1A, papain C-terminal	PRINTS	PR00705	PAPAIN	coord: 288..298 score: 54.37 coord: 303..309 score: 73.06 coord: 146..161 score: 63.78
IPR000668	Peptidase C1A, papain C-terminal	SMART	SM00645	pept_c1	coord: 128..343 e-value: 1.3E-119 score: 413.4
IPR000668	Peptidase C1A, papain C-terminal	PFAM	PF00112	Peptidase_C1	coord: 128..343 e-value: 1.8E-79 score: 266.9
IPR000118	Granulin	SMART	SM00277	GRAN_2	coord: 366..423 e-value: 6.6E-31 score: 118.7
IPR000118	Granulin	PFAM	PF00396	Granulin	coord: 377..423 e-value: 3.6E-11 score: 43.2
IPR013201	Cathepsin propeptide inhibitor domain (I29)	SMART	SM00848	Inhibitor_I29_2	coord: 41..98 e-value: 4.0E-17 score: 72.9
IPR013201	Cathepsin propeptide inhibitor domain (I29)	PFAM	PF08246	Inhibitor_I29	coord: 41..98 e-value: 1.9E-10 score: 41.0
IPR037277	Granulin superfamily	GENE3D	2.10.25.160	Granulin	coord: 365..435 e-value: 3.8E-13 score: 51.5
None	No IPR available	GENE3D	3.90.70.10	Cysteine proteinases	coord: 28..345 e-value: 2.2E-112 score: 377.7
None	No IPR available	PANTHER	PTHR12411:SF749	CYSTEINE PROTEASE	coord: 25..372
None	No IPR available	PANTHER	PTHR12411	CYSTEINE PROTEASE FAMILY C1-RELATED	coord: 25..372
None	No IPR available	SUPERFAMILY	57277	Granulin repeat	coord: 363..397
IPR000169	Cysteine peptidase, cysteine active site	PROSITE	PS00139	THIOL_PROTEASE_CYS	coord: 146..157
IPR025660	Cysteine peptidase, histidine active site	PROSITE	PS00639	THIOL_PROTEASE_HIS	coord: 286..296
IPR025661	Cysteine peptidase, asparagine active site	PROSITE	PS00640	THIOL_PROTEASE_ASN	coord: 303..322
IPR039417	Papain-like cysteine endopeptidase	CDD	cd02248	Peptidase_C1A	coord: 129..342 e-value: 2.22087E-104 score: 307.628
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 32..343

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy7G129860.1	Chy7G129860.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051603	proteolysis involved in cellular protein catabolic process
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005615	extracellular space
cellular_component	GO:0005764	lysosome
molecular_function	GO:0032440	2-alkenal reductase [NAD(P)+] activity
molecular_function	GO:0004197	cysteine-type endopeptidase activity
molecular_function	GO:0008234	cysteine-type peptidase activity