Chy6G127960 (gene) Cucumber (hystrix) v1

BLAST of Chy6G127960 vs. ExPASy Swiss-Prot
Match: Q9SEY5 (Isovalerate--CoA ligase AAE2 OS=Arabidopsis thaliana OX=3702 GN=AAE2 PE=1 SV=1)

HSP 1 Score: 465.3 bits (1196), Expect = 9.4e-130
Identity = 261/564 (46.28%), Postives = 359/564 (63.65%), Query Frame = 0

Query: 1   MEGFYHCPANFGPLSPVPFLKRASTLYGCRPSLVYGTRVFSWSDTYARSLALASALLHHF 60
           +EG    PANF PLSP+ FL+R++ +Y  R SLV+G+   +W  TY R L LASAL  + 
Sbjct: 48  IEGLLRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALT-NL 107

Query: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120
            +S  D+V A+APN+P ++ELHFAVPMAG I+  LNT+LD  TLS+LL     K++F+D 
Sbjct: 108 GISRGDVVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDH 167

Query: 121 QFLPILLKSLE-----NNSIKFPALVLI-----PSDPDTPLPSEF-----LDYNKVLAMR 180
           Q L I   +L+     + + K   LVLI       D D    S F      DY     ++
Sbjct: 168 QLLEIAHGALDLLAKSDKTRKSLKLVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLLK 227

Query: 181 FGDDDF-TPRPNAELDPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSP 240
            GD +F   +P  E DPISINYTSG+T   KGV+YSHR AYLNSLAT+F  ++   +  P
Sbjct: 228 SGDSEFEIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQM---SVYP 287

Query: 241 VFLWTVDMFRCNGWCFIWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPPTLLKMI 300
           V+LWTV MF CNGWC +W +AA GG NICLR V+   IF N+ +H+VT + G PT+L MI
Sbjct: 288 VYLWTVPMFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMI 347

Query: 301 SESSSNNCMPRRLSRRVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWK 360
              +     P  L  RV+++  G+ P+ +IL K+ ELGFN+S+ YG+TE  GP     WK
Sbjct: 348 VNYTVTEHKP--LPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWK 407

Query: 361 PTFEEDNVQFEDLITSLE---------IDVKDPISMESVLGDGETLGEVMLRGNTLMSGY 420
           P ++  +++    + + +         +DVKDP++ME+V  DG T+GEVM RGNT+MSGY
Sbjct: 408 PEWDSLSLEERTKLKARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGY 467

Query: 421 YKNMKATHEAFIGENWYRTGDVGVRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLM 480
           +K+++AT +AF G+ W+ +GD+ V++  G IE+KDR KD+++   G   +S+VEVE VL 
Sbjct: 468 FKDIEATRKAFEGD-WFHSGDLAVKYPDGYIEIKDRLKDVII--SGGENISSVEVERVLC 527

Query: 481 SHPSVAEVAVVGE------RTLYGFVKLKNGGNE-NGDEIVEFCRMHLPEFMVPKRIVFG 533
           SH +V E AVV        +T  GFVKLK G +    +EI+ FCR HLP +M PK IVFG
Sbjct: 528 SHQAVLEAAVVARPDHHWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFG 587

BLAST of Chy6G127960 vs. ExPASy Swiss-Prot
Match: M4IS92 (Probable CoA ligase CCL13 OS=Humulus lupulus OX=3486 GN=CCL13 PE=2 SV=1)

HSP 1 Score: 462.2 bits (1188), Expect = 7.9e-129
Identity = 263/555 (47.39%), Postives = 348/555 (62.70%), Query Frame = 0

Query: 1   MEGFYHCPANFGPLSPVPFLKRASTLYGCRPSLVYGTRVFSWSDTYARSLALASALLHHF 60
           MEG   C AN  PLSP+ FL+R+S  Y    SLVYG+  ++W+ T+ R L LASAL  HF
Sbjct: 29  MEGLVQCSANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASALTTHF 88

Query: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120
            +SP D+V   + NIPE+YELHFAVPMAG I+  LN + DS  +S LL     K+IF++ 
Sbjct: 89  GISPGDVVATFSYNIPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLIFVEP 148

Query: 121 QFLP---ILLKSLENNSIKFPALVLIPSDPDTPLPSEFLDYNKVLAMRFGDDDF-TPRPN 180
           Q L      L  L    IK P LVL+ +D ++   S +  YN +LA   G DDF   RP 
Sbjct: 149 QLLETARAALDLLAQKDIKPPTLVLL-TDSESFTSSSYDHYNHLLAN--GSDDFEIRRPK 208

Query: 181 AELDPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCN 240
            E DPISINYTSG+T   K V+YSHR AYLNS+AT+    +    +  V+LW+V MF CN
Sbjct: 209 NECDPISINYTSGTTARPKAVVYSHRGAYLNSIATVL---LHGMGTRSVYLWSVPMFHCN 268

Query: 241 GWCFIWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPPTLLKMISESSSNNCMPRR 300
           GWCF W  AA G  NIC+R V+  AIF N+ LH+VT     PT+L MI  S   N +   
Sbjct: 269 GWCFPWGAAAQGATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGN-LHTP 328

Query: 301 LSRRVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPTFEEDNVQFED 360
           L  +V+++  G+ P  +++ ++ E+GF +++ YG+TE  GPA     KP  E D +Q E+
Sbjct: 329 LPHKVEVMTGGSPPPPKVIARMEEMGFQVNHIYGLTETHGPATNCVCKP--EWDALQPEE 388

Query: 361 L-----------ITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNMKATHEA 420
                       +   E+DV+DP+SMESV  DG T+GEVM RGNT+MSGY+K++KAT EA
Sbjct: 389 RYALKARQGLNHLAMEEMDVRDPVSMESVRADGTTIGEVMFRGNTVMSGYFKDLKATEEA 448

Query: 421 FIGENWYRTGDVGVRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPSVAEVAV 480
           F G  W+RTGD+GV+H+ G I++KDR KD+V+   G   VSTVEVE VL SH +V E AV
Sbjct: 449 FEG-GWFRTGDLGVKHEDGYIQLKDRKKDVVI--SGGENVSTVEVETVLYSHEAVLEAAV 508

Query: 481 VGE------RTLYGFVKLKNG--GNENGDEIVEFCRMHLPEFMVPKRIVFGDLPMNSTGK 533
           V         T   FV LK G     + D+I++FCR  LP +M PK +VF +LP  STGK
Sbjct: 509 VARPDKLWGETPCAFVTLKEGFDNGVSADQIIKFCRDRLPHYMAPKTVVFEELPKTSTGK 568

BLAST of Chy6G127960 vs. ExPASy Swiss-Prot
Match: F4HUK6 (Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 3.3e-127
Identity = 257/557 (46.14%), Postives = 345/557 (61.94%), Query Frame = 0

Query: 1   MEGFYHCPANFGPLSPVPFLKRASTLYGCRPSLVYGTRVFSWSDTYARSLALASALLHHF 60
           MEG    PAN+ PL+P+ FL R++ +Y  R S+VYG+  ++W  T  R + +ASA L   
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASA-LSQL 62

Query: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120
            +S  D+V  +APN+P + ELHF VPMAGA++  LN + DS  +++LL+    KVIF D 
Sbjct: 63  GISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADH 122

Query: 121 QFLPI---LLKSLENNSIKFPALVLIPSDPDTPLPS------EFLDYNKVLAMRFGDDDF 180
           QFL I     + L N   K P LVLIP +P T   S      E ++Y  V+AM  G  DF
Sbjct: 123 QFLQIAEGACEILSNKGDKVPILVLIP-EPLTQSVSRKKRSEEMMEYEDVVAM--GKSDF 182

Query: 181 -TPRPNAELDPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTV 240
              RP  E D IS+NYTSG+T   KGV+YSHR AYLNSLA +  +++    SSP +LWT 
Sbjct: 183 EVIRPTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEM---HSSPTYLWTN 242

Query: 241 DMFRCNGWCFIWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPPTLLKMISESSSN 300
            MF CNGWC +W + A+GG NICLR VTA AIF N+  H+VT + G PT+L MI  +  +
Sbjct: 243 PMFHCNGWCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPES 302

Query: 301 NCMPRRLSRRVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPTF--- 360
              P  L  +V  I   A P   ++ K+ ELGF++ + YG+TE  GP  I  WKP +   
Sbjct: 303 EQKP--LPGKVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSL 362

Query: 361 ---EEDNVQFEDLITSL---EIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNMKA 420
              E+  ++    +  L   EI VKDP++M ++  DG T+GEV+ RGNT+M+GY KN +A
Sbjct: 363 PREEQAKMKARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEA 422

Query: 421 THEAFIGENWYRTGDVGVRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPSVA 480
           T EAF G  W+ +GD+GV+H  G IE+KDR+KDI++   G   +S++EVE  L +HP V 
Sbjct: 423 TKEAFKG-GWFWSGDLGVKHPDGYIELKDRSKDIII--SGGENISSIEVESTLFTHPCVL 482

Query: 481 EVAVVGE------RTLYGFVKLKNGGNENGDEIVEFCRMHLPEFMVPKRIVFGDLPMNST 533
           E AVV         T   FVKLK+G   + +E++ +CR  LP +M P+ IVF DLP  ST
Sbjct: 483 EAAVVARPDEYWGETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTST 542

BLAST of Chy6G127960 vs. ExPASy Swiss-Prot
Match: M4IRL4 (Isovalerate--CoA ligase CCL2 OS=Humulus lupulus OX=3486 GN=CCL2 PE=1 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 1.3e-126
Identity = 257/555 (46.31%), Postives = 348/555 (62.70%), Query Frame = 0

Query: 1   MEGFYHCPANFGPLSPVPFLKRASTLYGCRPSLVYGTRVFSWSDTYARSLALASALLHHF 60
           MEG   C AN  PLSP+ FL+R+S  Y    SLVYG+  ++W+ T+ R L LASAL  H 
Sbjct: 29  MEGLVQCSANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASALTTHL 88

Query: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120
            +SP D+V   + N+PE+YELHFAVPMAG I+  LN + DS  +S LL     K+IF++ 
Sbjct: 89  GISPGDVVATFSYNLPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLIFVEP 148

Query: 121 QFLP---ILLKSLENNSIKFPALVLIPSDPDTPLPSEFLDYNKVLAMRFGDDDF-TPRPN 180
           Q L      L  L    IK P LVL+ +D ++   S +  YN +LA   G DDF   RP 
Sbjct: 149 QLLETARAALDLLAQKDIKPPTLVLL-TDSESFTSSSYDHYNHLLAN--GSDDFEIRRPK 208

Query: 181 AELDPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCN 240
            E DPISINYTSG+T   K V+YSHR AYLNS+AT+    +    ++ V+LW+V MF CN
Sbjct: 209 NEWDPISINYTSGTTARPKAVVYSHRGAYLNSIATVL---LHGMGTTSVYLWSVPMFHCN 268

Query: 241 GWCFIWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPPTLLKMISESSSNNCMPRR 300
           GWCF W  AA G  NIC+R V+  AIF N+ LH+VT     PT+L MI  S   N +   
Sbjct: 269 GWCFPWGAAAQGATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGN-LHTP 328

Query: 301 LSRRVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPTFEEDNVQFED 360
           L  +V+++  G+ P  +++ ++ E+GF +++ YG+TE  GPA     KP  E D +Q E+
Sbjct: 329 LPHKVEVMTGGSPPPPKVIARMEEMGFQVNHIYGLTETCGPAANCVCKP--EWDALQPEE 388

Query: 361 L-----------ITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNMKATHEA 420
                       +   E+DV+DP++MESV  DG T+GEVM RGNT+MSGY+K++KAT EA
Sbjct: 389 RYALKARQGLNHLAMEEMDVRDPVTMESVRADGATIGEVMFRGNTVMSGYFKDLKATEEA 448

Query: 421 FIGENWYRTGDVGVRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPSVAEVAV 480
           F G  W+R+GD+GV+H+ G I++KDR KD+V+   G   +STVEVE VL SH +V E AV
Sbjct: 449 FEG-GWFRSGDLGVKHEDGYIQLKDRKKDVVI--SGGENISTVEVETVLYSHEAVLEAAV 508

Query: 481 VGE------RTLYGFVKLKNG--GNENGDEIVEFCRMHLPEFMVPKRIVFGDLPMNSTGK 533
           V         T   FV LK G   + + D+I++FCR  LP +M PK +VF +LP  STGK
Sbjct: 509 VARPDKLWGETPCAFVTLKEGFDNDVSADQIIKFCRDRLPHYMAPKTVVFEELPKTSTGK 568

BLAST of Chy6G127960 vs. ExPASy Swiss-Prot
Match: Q9C8D4 (Butyrate--CoA ligase AAE11, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE11 PE=1 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 5.9e-108
Identity = 225/561 (40.11%), Postives = 344/561 (61.32%), Query Frame = 0

Query: 1   MEGFYHCPANFGPLSPVPFLKRASTLYGCRPSLVYGTRVFSWSDTYARSLALASALLHHF 60
           M+    C AN  PL+P+ FLKRAS  Y  R S++YG   F+W  TY R   LA++LL   
Sbjct: 1   MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLL-SL 60

Query: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120
           +++  D+V  +APN+P +YE+HF+VPM GA+++ +NT+LD+ T++++L+   PK++F+D 
Sbjct: 61  NITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDY 120

Query: 121 QFLPILLKSLE----NNSIKFPALVLIPSDPDTPLP-SEFLDYNKVLAMRFGDDDFTPRP 180
           +F P++ + L       S   P ++LI     T  P S+ LDY  ++  R G+   TP  
Sbjct: 121 EFAPLIQEVLRLIPTYQSQPHPRIILINEIDSTTKPFSKELDYEGLI--RKGEP--TPSS 180

Query: 181 NA-------ELDPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLW 240
           +A       E DPIS+NYTSG+T   KGV+ SH+ AYL++L++I   ++      PV+LW
Sbjct: 181 SASMFRVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEM---GIFPVYLW 240

Query: 241 TVDMFRCNGWCFIWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPPTLLKMISESS 300
           T+ MF CNGW   W +AA GG N+C+R VTA  I+ N+ELH VT +   PT+ + + E S
Sbjct: 241 TLPMFHCNGWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGS 300

Query: 301 SNNCMPRRLSRRVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPTF- 360
             +  P+  S  V ++  G+ P   ++ KV +LGF++ +GYG+TEA GP +   W+  + 
Sbjct: 301 RTDQSPK--SSPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWN 360

Query: 361 ---EEDNVQFEDL-----ITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNM 420
              E   ++ +       +T  ++DVK+  ++ESV  DG+T+GE++++G++LM GY KN 
Sbjct: 361 KLPEHQQIELQQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNP 420

Query: 421 KATHEAFIGENWYRTGDVGVRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPS 480
           KAT EAF    W  TGD+GV H  G +E+KDR+KDI++   G   +S++EVE VL  +  
Sbjct: 421 KATSEAF-KHGWLNTGDIGVIHPDGYVEIKDRSKDIII--SGGENISSIEVEKVLYMYQE 480

Query: 481 VAEVAVVG-ERTLYG-----FVKLKNGGN---ENGDEIVEFCRMHLPEFMVPKRIV-FGD 531
           V E AVV     L+G     FV LK G      +  +++++CR ++P FM PK++V F +
Sbjct: 481 VLEAAVVAMPHPLWGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQE 540

BLAST of Chy6G127960 vs. ExPASy TrEMBL
Match: A0A1S3BKP8 (probable acyl-activating enzyme 2 OS=Cucumis melo OX=3656 GN=LOC103490658 PE=4 SV=1)

HSP 1 Score: 1007.7 bits (2604), Expect = 1.9e-290
Identity = 501/542 (92.44%), Postives = 519/542 (95.76%), Query Frame = 0

Query: 1   MEGFYHCPANFGPLSPVPFLKRASTLYGCRPSLVYGTRVFSWSDTYARSLALASALLHHF 60
           MEGF+HCP NF PLSPVPFLKRASTLYGCRPSL+YG+RVF+WS TY R L++ASAL+HHF
Sbjct: 1   MEGFHHCPPNFAPLSPVPFLKRASTLYGCRPSLLYGSRVFTWSQTYGRCLSIASALVHHF 60

Query: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120
           HLSPADLVVAMAPNIPELYELHFAVPMAG IISALNTKLDSPTLSLLLQQLNPKVIFLDS
Sbjct: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120

Query: 121 QFLPILLKSLENNSIKFPALVLIPSDPDTPLPSEFLDYNKVLAMRFGDDDFTPRPNAELD 180
           QFLPILLKSLEN SIKFPAL+LIPSDP+T LPS+FLDYNK+L MR G D+FTPRPNAELD
Sbjct: 121 QFLPILLKSLENISIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNAELD 180

Query: 181 PISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCF 240
           PISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCF
Sbjct: 181 PISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCF 240

Query: 241 IWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPPTLLKMISESSSNNCMPRRLSRR 300
           IWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGP TLLKMI ESSSNNCMPRRLSRR
Sbjct: 241 IWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRLSRR 300

Query: 301 VDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPTFE-EDNVQFED-LI 360
           VDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKP+FE +DNVQF+D LI
Sbjct: 301 VDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDDHLI 360

Query: 361 TSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNMKATHEAFIGENWYRTGDVG 420
           TSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKN+KATHEAF+GENWYRTGDVG
Sbjct: 361 TSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTGDVG 420

Query: 421 VRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPSVAEVAVVGERTLYGFVKLK 480
           VRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHP+VAE AVVGERTLYGFVKLK
Sbjct: 421 VRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVGERTLYGFVKLK 480

Query: 481 NGGNENGDEIVEFCRMHLPEFMVPKRIVFGDLPMNSTGKVQKFVLREKAKALFSNANNNT 540
           NG  EN DEIVEFCRMHLPEFMVPK IVFGDLPMNSTGKVQKFVLREKAKAL  N NNNT
Sbjct: 481 NGSKENNDEIVEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKALL-NGNNNT 540

BLAST of Chy6G127960 vs. ExPASy TrEMBL
Match: A0A0A0L3L0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G002400 PE=4 SV=1)

HSP 1 Score: 922.2 bits (2382), Expect = 1.0e-264
Identity = 457/470 (97.23%), Postives = 462/470 (98.30%), Query Frame = 0

Query: 71  MAPNIPELYELHFAVPMAGAIISALNTKLDSPTLSLLLQQLNPKVIFLDSQFLPILLKSL 130
           MAPNIPELYELHFAVPMAGAIISALNTKLDSPTLSLLLQQLNPKVIF+DSQFLPILLKSL
Sbjct: 1   MAPNIPELYELHFAVPMAGAIISALNTKLDSPTLSLLLQQLNPKVIFIDSQFLPILLKSL 60

Query: 131 ENNSIKFPALVLIPSDPDTPLPSEFLDYNKVLAMRFGDDDFTPRPNAELDPISINYTSGS 190
           EN+SIKFPALVLIPSDPDTPLPSEFLDYNKVLAMRFGDDDFTPRPNAELDPISINYTSGS
Sbjct: 61  ENSSIKFPALVLIPSDPDTPLPSEFLDYNKVLAMRFGDDDFTPRPNAELDPISINYTSGS 120

Query: 191 TGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAALGGC 250
           TGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAALGGC
Sbjct: 121 TGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAALGGC 180

Query: 251 NICLRTVTADAIFTNVELHRVTLLCGPPTLLKMISESSSNNCMPRRLSRRVDLIVAGALP 310
           NICLRTVTADAIFTNVELHRVTLLCGPPTLLKMI ESSSNNCMPRRLSRRVDLIVAGALP
Sbjct: 181 NICLRTVTADAIFTNVELHRVTLLCGPPTLLKMIYESSSNNCMPRRLSRRVDLIVAGALP 240

Query: 311 IKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPTFEEDNVQFEDLITSLEIDVKDPIS 370
           IKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPTFEEDNVQFEDLITSLEIDVKDPIS
Sbjct: 241 IKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPTFEEDNVQFEDLITSLEIDVKDPIS 300

Query: 371 MESVLGDGETLGEVMLRGNTLMSGYYKNMKATHEAFIGENWYRTGDVGVRHKSGRIEMKD 430
           MESVLGDGETLGEVMLRGNTLMSGYYKNMKATHEAFIGENWYRTGDVGVRHKSGRIEMKD
Sbjct: 301 MESVLGDGETLGEVMLRGNTLMSGYYKNMKATHEAFIGENWYRTGDVGVRHKSGRIEMKD 360

Query: 431 RAKDIVVRTDGEGAVSTVEVEGVLMSHPSVAEVAVVGERTLYGFVKLKNGGNENGDEIVE 490
           RAKDIVVRTDGEGAVSTVEVEGVLMSHP+VAE AVVGERTLYGFVKLKN   ENGDEIVE
Sbjct: 361 RAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAEAAVVGERTLYGFVKLKNRSKENGDEIVE 420

Query: 491 FCRMHLPEFMVPKRIVFGDLPMNSTGKVQKFVLREKAKALFSNANNNTIT 541
           FCRMHLPEFM+PKRIVFGDLPMNSTGKVQKF LREK KAL +NANNNTIT
Sbjct: 421 FCRMHLPEFMIPKRIVFGDLPMNSTGKVQKFALREKVKALLNNANNNTIT 470

BLAST of Chy6G127960 vs. ExPASy TrEMBL
Match: A0A5A7UEI8 (Putative acyl-activating enzyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G00360 PE=4 SV=1)

HSP 1 Score: 881.3 bits (2276), Expect = 2.0e-252
Identity = 443/472 (93.86%), Postives = 454/472 (96.19%), Query Frame = 0

Query: 71  MAPNIPELYELHFAVPMAGAIISALNTKLDSPTLSLLLQQLNPKVIFLDSQFLPILLKSL 130
           MAPNIPELYELHFAVPMAG IISALNTKLDSPTLSLLLQQLNPKVIFLDSQFLPILLKSL
Sbjct: 1   MAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDSQFLPILLKSL 60

Query: 131 ENNSIKFPALVLIPSDPDTPLPSEFLDYNKVLAMRFGDDDFTPRPNAELDPISINYTSGS 190
           EN SIKFPAL+LIPSDP+T LPS+FLDYNK+L MR G D+FTPRPNAELDPISINYTSGS
Sbjct: 61  ENISIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNAELDPISINYTSGS 120

Query: 191 TGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAALGGC 250
           TGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAALGGC
Sbjct: 121 TGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAALGGC 180

Query: 251 NICLRTVTADAIFTNVELHRVTLLCGPPTLLKMISESSSNNCMPRRLSRRVDLIVAGALP 310
           NICLRTVTADAIFTNVELHRVTLLCGP TLLKMI ESSSNNCMPRRLSRRVDLIVAGALP
Sbjct: 181 NICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRLSRRVDLIVAGALP 240

Query: 311 IKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPTFE-EDNVQFED-LITSLEIDVKDP 370
           IKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKP+FE +DNVQF+D LITSLEIDVKDP
Sbjct: 241 IKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDDHLITSLEIDVKDP 300

Query: 371 ISMESVLGDGETLGEVMLRGNTLMSGYYKNMKATHEAFIGENWYRTGDVGVRHKSGRIEM 430
           ISMESVLGDGETLGEVMLRGNTLMSGYYKN+KATHEAF+GENWYRTGDVGVRHKSGRIEM
Sbjct: 301 ISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTGDVGVRHKSGRIEM 360

Query: 431 KDRAKDIVVRTDGEGAVSTVEVEGVLMSHPSVAEVAVVGERTLYGFVKLKNGGNENGDEI 490
           KDRAKDIVVRTDGEGAVSTVEVEGVLMSHP+VAE AVVGERTLYGFVKLKNG  EN DEI
Sbjct: 361 KDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVGERTLYGFVKLKNGSKENNDEI 420

Query: 491 VEFCRMHLPEFMVPKRIVFGDLPMNSTGKVQKFVLREKAKALFSNANNNTIT 541
           VEFCRMHLPEFMVPK IVFGDLPMNSTGKVQKFVLREKAKAL  N NNNTIT
Sbjct: 421 VEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKALL-NGNNNTIT 471

BLAST of Chy6G127960 vs. ExPASy TrEMBL
Match: A0A6J1E5I3 (probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111430963 PE=4 SV=1)

HSP 1 Score: 855.1 bits (2208), Expect = 1.6e-244
Identity = 431/537 (80.26%), Postives = 474/537 (88.27%), Query Frame = 0

Query: 1   MEGFYHCPANFGPLSPVPFLKRASTLYGCRPSLVYGTRVFSWSDTYARSLALASALLHHF 60
           M+ F   PANF PLSPV FLK+++ +Y  RPS++YG+RVF+WS+TY RSL LASAL+HHF
Sbjct: 49  MDPFSPSPANFVPLSPVTFLKKSAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 108

Query: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120
           H+SP D+V AMAPN+PELYELHFAVPMAGAIIS LNTKLD+PTLSLLLQQL+PK+IFLDS
Sbjct: 109 HVSPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 168

Query: 121 QFLPILLKSLE---NNSIKFPALVLIPSDPDTPLPSEFLDYNKVLAMRFGDDDFTPRPNA 180
            FLP +L+SL     NSI+FPALVLIP+ PDTP PSEFLDYN+VL MRF  DDFTPR NA
Sbjct: 169 DFLPTVLQSLSQTYGNSIQFPALVLIPAHPDTP-PSEFLDYNRVLGMRF--DDFTPRTNA 228

Query: 181 ELDPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNG 240
           ELD ISIN TSGSTGLHKGV+YSHRAAYLNSLATIFRS IC +TSSPVFLWTVDMFRCNG
Sbjct: 229 ELDAISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNG 288

Query: 241 WCFIWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPPTLLKMISESSSNNCMPRRL 300
           WCFIW MAALGGCNICLR VT DAIFTN+ELH+VTLLCGP TLLKMI ESSS N     L
Sbjct: 289 WCFIWAMAALGGCNICLRNVTTDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWL 348

Query: 301 SRRVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPTFEEDNVQFEDL 360
           SRRVDLIVAGALPI EILTKV ELGFNISYGYGMTEAMGPA+IRPWKP F+E+ VQFEDL
Sbjct: 349 SRRVDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDL 408

Query: 361 ITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNMKATHEAFIGENWYRTGDV 420
           ITSLEIDVKDP+SMESVLGDGETLGEVMLRGNTLMSGYYKN+KAT EAF G+ WYRTGDV
Sbjct: 409 ITSLEIDVKDPLSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGD-WYRTGDV 468

Query: 421 GVRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPSVAEVAVVGERTLYGFVKL 480
           GVRHKSGRIEMKDRAKDIVVR +GE AVSTV+VE VLMSHPSVAE AVVGER L GFVKL
Sbjct: 469 GVRHKSGRIEMKDRAKDIVVRANGEVAVSTVQVEAVLMSHPSVAEAAVVGER-LCGFVKL 528

Query: 481 KNGGNENGDEIVEFCRMHLPEFMVPKRIVFGDLPMNSTGKVQKFVLREKAKALFSNA 535
           KNG   + +EIV+FCR HLPEFM+P+R+VFGDLPMNS GKVQKF+ REKAKAL +N+
Sbjct: 529 KNGSRASAEEIVKFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAKALNTNS 580

BLAST of Chy6G127960 vs. ExPASy TrEMBL
Match: A0A6J1L3K3 (probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111500216 PE=4 SV=1)

HSP 1 Score: 848.2 bits (2190), Expect = 1.9e-242
Identity = 431/540 (79.81%), Postives = 473/540 (87.59%), Query Frame = 0

Query: 1   MEGFYHCPANFGPLSPVPFLKRASTLYGCRPSLVYGTRVFSWSDTYARSLALASALLHHF 60
           M+ F+  PANF PLSP+ F KRA+ +YG RPS++YG+RVF+WS+TY R L LASAL+HHF
Sbjct: 42  MDPFFPSPANFVPLSPLAFFKRAAAVYGGRPSVIYGSRVFTWSETYGRCLHLASALVHHF 101

Query: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120
           H+SP D+V AMAPN+PELYELHFAVPMAGAIIS LNTKLD+PTLSLLLQQL+PKVIFLDS
Sbjct: 102 HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKVIFLDS 161

Query: 121 QFLPILLKSLE---NNSIKFPALVLIPSDPDTPLPSEFLDYNKVLAMRFGDDDFTPRPNA 180
            FLP LL+SL     NSI+FPALVLIP+ PDTP PSEFLDYN+VL MRF  D+FTPR N+
Sbjct: 162 DFLPTLLQSLSQTYGNSIQFPALVLIPAHPDTP-PSEFLDYNRVLEMRF--DEFTPRTNS 221

Query: 181 ELDPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNG 240
           ELD ISIN TSGSTGLHKGV+YSHRAAYLNSLATIFRS IC +TSSPVFLWTVDMFRCNG
Sbjct: 222 ELDAISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNG 281

Query: 241 WCFIWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPPTLLKMISESSS-NNCMPRR 300
           WCFIW MAALGGCNICLR VT DAIFTN+ELH+VTLLCGP TLLKMI ESSS NNC P  
Sbjct: 282 WCFIWAMAALGGCNICLRNVTPDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNCKPGF 341

Query: 301 LSRRVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPTFEEDNVQFED 360
           L RRVDLIVAGALPI EILTKV ELGFNISYGYGMTEAMGPA+IRPWKP F+E+ VQFED
Sbjct: 342 L-RRVDLIVAGALPIDEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFED 401

Query: 361 LITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNMKATHEAFIGENWYRTGD 420
           LI SLEIDVKDP SMESVLGDGETLGEVMLRGN+LMSGYYKN+KAT EAF G+ WYRTGD
Sbjct: 402 LIASLEIDVKDPSSMESVLGDGETLGEVMLRGNSLMSGYYKNLKATGEAFSGD-WYRTGD 461

Query: 421 VGVRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPSVAEVAVVGERTLYGFVK 480
           VGV+HKSGRIEMKDRAKDIVVR +GEGAVSTV+VE VLMSHP VAE AV+GER L G VK
Sbjct: 462 VGVKHKSGRIEMKDRAKDIVVRENGEGAVSTVQVEAVLMSHPGVAEAAVIGER-LCGLVK 521

Query: 481 LKNGGNENGDEIVEFCRMHLPEFMVPKRIVFGDLPMNSTGKVQKFVLREKAKALFSNANN 537
           LKNG   + +EIVEFCR HLPEFM+P+R+VFGDLPMNS GKVQKF+ REKAKAL  N NN
Sbjct: 522 LKNGSRASAEEIVEFCRAHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAKAL--NTNN 573

BLAST of Chy6G127960 vs. NCBI nr
Match: XP_004146208.2 (probable acyl-activating enzyme 2 [Cucumis sativus] >KAE8649992.1 hypothetical protein Csa_010505 [Cucumis sativus])

HSP 1 Score: 1066 bits (2756), Expect = 0.0
Identity = 526/540 (97.41%), Postives = 532/540 (98.52%), Query Frame = 0

Query: 1   MEGFYHCPANFGPLSPVPFLKRASTLYGCRPSLVYGTRVFSWSDTYARSLALASALLHHF 60
           MEGFYHCPANFGPLSPVPFLKRASTLYGCRPSLVYGTR+FSWSDTYARSLALASALLHHF
Sbjct: 1   MEGFYHCPANFGPLSPVPFLKRASTLYGCRPSLVYGTRLFSWSDTYARSLALASALLHHF 60

Query: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120
           HLSPADLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDSPTLSLLLQQLNPKVIF+DS
Sbjct: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDSPTLSLLLQQLNPKVIFIDS 120

Query: 121 QFLPILLKSLENNSIKFPALVLIPSDPDTPLPSEFLDYNKVLAMRFGDDDFTPRPNAELD 180
           QFLPILLKSLEN+SIKFPALVLIPSDPDTPLPSEFLDYNKVLAMRFGDDDFTPRPNAELD
Sbjct: 121 QFLPILLKSLENSSIKFPALVLIPSDPDTPLPSEFLDYNKVLAMRFGDDDFTPRPNAELD 180

Query: 181 PISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCF 240
           PISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCF
Sbjct: 181 PISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCF 240

Query: 241 IWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPPTLLKMISESSSNNCMPRRLSRR 300
           IWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPPTLLKMI ESSSNNCMPRRLSRR
Sbjct: 241 IWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPPTLLKMIYESSSNNCMPRRLSRR 300

Query: 301 VDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPTFEEDNVQFEDLITS 360
           VDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPTFEEDNVQFEDLITS
Sbjct: 301 VDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPTFEEDNVQFEDLITS 360

Query: 361 LEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNMKATHEAFIGENWYRTGDVGVR 420
           LEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNMKATHEAFIGENWYRTGDVGVR
Sbjct: 361 LEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNMKATHEAFIGENWYRTGDVGVR 420

Query: 421 HKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPSVAEVAVVGERTLYGFVKLKNG 480
           HKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHP+VAE AVVGERTLYGFVKLKN 
Sbjct: 421 HKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAEAAVVGERTLYGFVKLKNR 480

Query: 481 GNENGDEIVEFCRMHLPEFMVPKRIVFGDLPMNSTGKVQKFVLREKAKALFSNANNNTIT 540
             ENGDEIVEFCRMHLPEFM+PKRIVFGDLPMNSTGKVQKF LREK KAL +NANNNTIT
Sbjct: 481 SKENGDEIVEFCRMHLPEFMIPKRIVFGDLPMNSTGKVQKFALREKVKALLNNANNNTIT 540

BLAST of Chy6G127960 vs. NCBI nr
Match: XP_008448497.1 (PREDICTED: probable acyl-activating enzyme 2 [Cucumis melo])

HSP 1 Score: 1007 bits (2604), Expect = 0.0
Identity = 501/542 (92.44%), Postives = 519/542 (95.76%), Query Frame = 0

Query: 1   MEGFYHCPANFGPLSPVPFLKRASTLYGCRPSLVYGTRVFSWSDTYARSLALASALLHHF 60
           MEGF+HCP NF PLSPVPFLKRASTLYGCRPSL+YG+RVF+WS TY R L++ASAL+HHF
Sbjct: 1   MEGFHHCPPNFAPLSPVPFLKRASTLYGCRPSLLYGSRVFTWSQTYGRCLSIASALVHHF 60

Query: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120
           HLSPADLVVAMAPNIPELYELHFAVPMAG IISALNTKLDSPTLSLLLQQLNPKVIFLDS
Sbjct: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120

Query: 121 QFLPILLKSLENNSIKFPALVLIPSDPDTPLPSEFLDYNKVLAMRFGDDDFTPRPNAELD 180
           QFLPILLKSLEN SIKFPAL+LIPSDP+T LPS+FLDYNK+L MR G D+FTPRPNAELD
Sbjct: 121 QFLPILLKSLENISIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNAELD 180

Query: 181 PISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCF 240
           PISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCF
Sbjct: 181 PISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCF 240

Query: 241 IWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPPTLLKMISESSSNNCMPRRLSRR 300
           IWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGP TLLKMI ESSSNNCMPRRLSRR
Sbjct: 241 IWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRLSRR 300

Query: 301 VDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPTFEE-DNVQFED-LI 360
           VDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKP+FE+ DNVQF+D LI
Sbjct: 301 VDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDDHLI 360

Query: 361 TSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNMKATHEAFIGENWYRTGDVG 420
           TSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKN+KATHEAF+GENWYRTGDVG
Sbjct: 361 TSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTGDVG 420

Query: 421 VRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPSVAEVAVVGERTLYGFVKLK 480
           VRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHP+VAE AVVGERTLYGFVKLK
Sbjct: 421 VRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVGERTLYGFVKLK 480

Query: 481 NGGNENGDEIVEFCRMHLPEFMVPKRIVFGDLPMNSTGKVQKFVLREKAKALFSNANNNT 540
           NG  EN DEIVEFCRMHLPEFMVPK IVFGDLPMNSTGKVQKFVLREKAKAL  N NNNT
Sbjct: 481 NGSKENNDEIVEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKALL-NGNNNT 540

BLAST of Chy6G127960 vs. NCBI nr
Match: XP_038903286.1 (probable acyl-activating enzyme 2 [Benincasa hispida])

HSP 1 Score: 928 bits (2398), Expect = 0.0
Identity = 465/537 (86.59%), Postives = 493/537 (91.81%), Query Frame = 0

Query: 1   MEGFYHCPANFGPLSPVPFLKRASTLYGCRPSLVYGTRVFSWSDTYARSLALASALLHHF 60
           MEGF+HCPANF PLSPV FLKRA+T+YG RPS+VYG RV++WS+TY R L LASAL+HHF
Sbjct: 1   MEGFFHCPANFTPLSPVGFLKRAATVYGGRPSVVYGARVYTWSETYGRCLTLASALVHHF 60

Query: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120
           H+SP DLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDS  LSLLLQQLNPK+IFLDS
Sbjct: 61  HVSPGDLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDSSALSLLLQQLNPKIIFLDS 120

Query: 121 QFLPILLKSLENNSIKFPALVLIPSDPDTPLPSEFLDYNKVLAMRFGDD-DFTPRPNAEL 180
           QFLPILLKSLENNSIKFPALVLI ++P+T    E+ DYN+VLAMRF DD DFTPR NAEL
Sbjct: 121 QFLPILLKSLENNSIKFPALVLISTNPNTSR-LEYFDYNRVLAMRFSDDNDFTPRSNAEL 180

Query: 181 DPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWC 240
           DPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRS IC STSSPVFLWTVDMFRCNGWC
Sbjct: 181 DPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSGICKSTSSPVFLWTVDMFRCNGWC 240

Query: 241 FIWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPPTLLKMISESSSNNCMPRRLSR 300
           FIW MAALGGCNICLRTVTA+AIFTNVELH+VTLLCGP TLLKMISES SN+ M RRLSR
Sbjct: 241 FIWAMAALGGCNICLRTVTAEAIFTNVELHKVTLLCGPSTLLKMISESLSNHYMSRRLSR 300

Query: 301 RVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPTFEEDNVQFEDLIT 360
           RVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGP IIRPWKP FEE+ VQFEDLIT
Sbjct: 301 RVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPVIIRPWKPNFEEETVQFEDLIT 360

Query: 361 SLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNMKATHEAFIGENWYRTGDVGV 420
           SLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKN+KATHEAF G+NWYRTGD+G+
Sbjct: 361 SLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFSGDNWYRTGDIGL 420

Query: 421 RHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPSVAEVAVVGERTLYGFVKLKN 480
           RHKSGRIEMKDRAKDIVVR DGEG VSTVEVEGVLMSHP VAE AVV ER L GFVK+K+
Sbjct: 421 RHKSGRIEMKDRAKDIVVRADGEGVVSTVEVEGVLMSHPDVAEAAVVEERMLCGFVKMKD 480

Query: 481 GGNENGDEIVEFCRMHLPEFMVPKRIVFGDLPMNSTGKVQKFVLREKAKALFSNANN 536
              ENGDEIVEFCRM+LPEFMVPK+IVFGDLPMNS GKVQKFV+REKAK L  NANN
Sbjct: 481 ERKENGDEIVEFCRMYLPEFMVPKKIVFGDLPMNSIGKVQKFVVREKAKTL--NANN 534

BLAST of Chy6G127960 vs. NCBI nr
Match: KAA0052927.1 (putative acyl-activating enzyme 2 [Cucumis melo var. makuwa] >TYK11384.1 putative acyl-activating enzyme 2 [Cucumis melo var. makuwa])

HSP 1 Score: 880 bits (2273), Expect = 0.0
Identity = 443/472 (93.86%), Postives = 454/472 (96.19%), Query Frame = 0

Query: 71  MAPNIPELYELHFAVPMAGAIISALNTKLDSPTLSLLLQQLNPKVIFLDSQFLPILLKSL 130
           MAPNIPELYELHFAVPMAG IISALNTKLDSPTLSLLLQQLNPKVIFLDSQFLPILLKSL
Sbjct: 1   MAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDSQFLPILLKSL 60

Query: 131 ENNSIKFPALVLIPSDPDTPLPSEFLDYNKVLAMRFGDDDFTPRPNAELDPISINYTSGS 190
           EN SIKFPAL+LIPSDP+T LPS+FLDYNK+L MR G D+FTPRPNAELDPISINYTSGS
Sbjct: 61  ENISIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNAELDPISINYTSGS 120

Query: 191 TGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAALGGC 250
           TGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAALGGC
Sbjct: 121 TGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAALGGC 180

Query: 251 NICLRTVTADAIFTNVELHRVTLLCGPPTLLKMISESSSNNCMPRRLSRRVDLIVAGALP 310
           NICLRTVTADAIFTNVELHRVTLLCGP TLLKMI ESSSNNCMPRRLSRRVDLIVAGALP
Sbjct: 181 NICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRLSRRVDLIVAGALP 240

Query: 311 IKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPTFEE-DNVQFED-LITSLEIDVKDP 370
           IKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKP+FE+ DNVQF+D LITSLEIDVKDP
Sbjct: 241 IKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDDHLITSLEIDVKDP 300

Query: 371 ISMESVLGDGETLGEVMLRGNTLMSGYYKNMKATHEAFIGENWYRTGDVGVRHKSGRIEM 430
           ISMESVLGDGETLGEVMLRGNTLMSGYYKN+KATHEAF+GENWYRTGDVGVRHKSGRIEM
Sbjct: 301 ISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTGDVGVRHKSGRIEM 360

Query: 431 KDRAKDIVVRTDGEGAVSTVEVEGVLMSHPSVAEVAVVGERTLYGFVKLKNGGNENGDEI 490
           KDRAKDIVVRTDGEGAVSTVEVEGVLMSHP+VAE AVVGERTLYGFVKLKNG  EN DEI
Sbjct: 361 KDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVGERTLYGFVKLKNGSKENNDEI 420

Query: 491 VEFCRMHLPEFMVPKRIVFGDLPMNSTGKVQKFVLREKAKALFSNANNNTIT 540
           VEFCRMHLPEFMVPK IVFGDLPMNSTGKVQKFVLREKAKAL  N NNNTIT
Sbjct: 421 VEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKALL-NGNNNTIT 471

BLAST of Chy6G127960 vs. NCBI nr
Match: KAG6577804.1 (putative acyl-activating enzyme 1, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 855 bits (2210), Expect = 2.02e-309
Identity = 432/537 (80.45%), Postives = 472/537 (87.90%), Query Frame = 0

Query: 1   MEGFYHCPANFGPLSPVPFLKRASTLYGCRPSLVYGTRVFSWSDTYARSLALASALLHHF 60
           M+ F   PANF PLSPV F KRA+ +Y  RPS++YG+RVF+WS+TY RSL LASAL+HHF
Sbjct: 1   MDPFSPSPANFLPLSPVTFFKRAAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 60

Query: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120
           H+SP D+V AMAPN+PELYELHFAVPMAGAIIS LNTKLD+PTLSLLLQQL+PK+IFLDS
Sbjct: 61  HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 120

Query: 121 QFLPILLKSLEN---NSIKFPALVLIPSDPDTPLPSEFLDYNKVLAMRFGDDDFTPRPNA 180
            FLP +LKSL     NSI+FPALVLIP+ PDTP PSEFLDYN+VL MRF  DDFTPR NA
Sbjct: 121 DFLPTVLKSLSQTYGNSIQFPALVLIPTHPDTP-PSEFLDYNRVLGMRF--DDFTPRTNA 180

Query: 181 ELDPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNG 240
           ELD ISIN TSGSTGLHKGV+YSHRAAYLNSLATIFRS IC +TSSPVFLWTVDMFRCNG
Sbjct: 181 ELDAISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNG 240

Query: 241 WCFIWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPPTLLKMISESSSNNCMPRRL 300
           WCFIW MAALGGCNICLR VTADAIFTN+ELH+VTLLCGP TLLKMI ESSS N      
Sbjct: 241 WCFIWAMAALGGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWF 300

Query: 301 SRRVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPTFEEDNVQFEDL 360
           SRRVDLIVAGALPI EILTKV ELGFNISYGYGMTEAMGPA+IRPWKP F+E+ VQFEDL
Sbjct: 301 SRRVDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEEIVQFEDL 360

Query: 361 ITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNMKATHEAFIGENWYRTGDV 420
            TSLEIDVKDP+SMESVLGDGETLGEVMLRGNTLMSGYYKN+KAT EAF G+ WYRTGDV
Sbjct: 361 FTSLEIDVKDPMSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGD-WYRTGDV 420

Query: 421 GVRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPSVAEVAVVGERTLYGFVKL 480
           GVRHKSGRIEMKDRAKDIVVR +GE AVSTV+VE VLMSHPSVAE AVVGER L GFVKL
Sbjct: 421 GVRHKSGRIEMKDRAKDIVVRANGEAAVSTVQVEAVLMSHPSVAEAAVVGER-LCGFVKL 480

Query: 481 KNGGNENGDEIVEFCRMHLPEFMVPKRIVFGDLPMNSTGKVQKFVLREKAKALFSNA 534
           KNG   + +EIVEFCR HLPEFM+P+R+VFGDLPMNS GKVQKF++REKAK L +N+
Sbjct: 481 KNGSRASAEEIVEFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKELNTNS 532

BLAST of Chy6G127960 vs. TAIR 10
Match: AT2G17650.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 465.3 bits (1196), Expect = 6.6e-131
Identity = 261/564 (46.28%), Postives = 359/564 (63.65%), Query Frame = 0

Query: 1   MEGFYHCPANFGPLSPVPFLKRASTLYGCRPSLVYGTRVFSWSDTYARSLALASALLHHF 60
           +EG    PANF PLSP+ FL+R++ +Y  R SLV+G+   +W  TY R L LASAL  + 
Sbjct: 48  IEGLLRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALT-NL 107

Query: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120
            +S  D+V A+APN+P ++ELHFAVPMAG I+  LNT+LD  TLS+LL     K++F+D 
Sbjct: 108 GISRGDVVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDH 167

Query: 121 QFLPILLKSLE-----NNSIKFPALVLI-----PSDPDTPLPSEF-----LDYNKVLAMR 180
           Q L I   +L+     + + K   LVLI       D D    S F      DY     ++
Sbjct: 168 QLLEIAHGALDLLAKSDKTRKSLKLVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLLK 227

Query: 181 FGDDDF-TPRPNAELDPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSP 240
            GD +F   +P  E DPISINYTSG+T   KGV+YSHR AYLNSLAT+F  ++   +  P
Sbjct: 228 SGDSEFEIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQM---SVYP 287

Query: 241 VFLWTVDMFRCNGWCFIWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPPTLLKMI 300
           V+LWTV MF CNGWC +W +AA GG NICLR V+   IF N+ +H+VT + G PT+L MI
Sbjct: 288 VYLWTVPMFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMI 347

Query: 301 SESSSNNCMPRRLSRRVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWK 360
              +     P  L  RV+++  G+ P+ +IL K+ ELGFN+S+ YG+TE  GP     WK
Sbjct: 348 VNYTVTEHKP--LPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWK 407

Query: 361 PTFEEDNVQFEDLITSLE---------IDVKDPISMESVLGDGETLGEVMLRGNTLMSGY 420
           P ++  +++    + + +         +DVKDP++ME+V  DG T+GEVM RGNT+MSGY
Sbjct: 408 PEWDSLSLEERTKLKARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGY 467

Query: 421 YKNMKATHEAFIGENWYRTGDVGVRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLM 480
           +K+++AT +AF G+ W+ +GD+ V++  G IE+KDR KD+++   G   +S+VEVE VL 
Sbjct: 468 FKDIEATRKAFEGD-WFHSGDLAVKYPDGYIEIKDRLKDVII--SGGENISSVEVERVLC 527

Query: 481 SHPSVAEVAVVGE------RTLYGFVKLKNGGNE-NGDEIVEFCRMHLPEFMVPKRIVFG 533
           SH +V E AVV        +T  GFVKLK G +    +EI+ FCR HLP +M PK IVFG
Sbjct: 528 SHQAVLEAAVVARPDHHWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFG 587

BLAST of Chy6G127960 vs. TAIR 10
Match: AT1G20560.1 (acyl activating enzyme 1 )

HSP 1 Score: 456.8 bits (1174), Expect = 2.4e-128
Identity = 257/557 (46.14%), Postives = 345/557 (61.94%), Query Frame = 0

Query: 1   MEGFYHCPANFGPLSPVPFLKRASTLYGCRPSLVYGTRVFSWSDTYARSLALASALLHHF 60
           MEG    PAN+ PL+P+ FL R++ +Y  R S+VYG+  ++W  T  R + +ASA L   
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASA-LSQL 62

Query: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120
            +S  D+V  +APN+P + ELHF VPMAGA++  LN + DS  +++LL+    KVIF D 
Sbjct: 63  GISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADH 122

Query: 121 QFLPI---LLKSLENNSIKFPALVLIPSDPDTPLPS------EFLDYNKVLAMRFGDDDF 180
           QFL I     + L N   K P LVLIP +P T   S      E ++Y  V+AM  G  DF
Sbjct: 123 QFLQIAEGACEILSNKGDKVPILVLIP-EPLTQSVSRKKRSEEMMEYEDVVAM--GKSDF 182

Query: 181 -TPRPNAELDPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTV 240
              RP  E D IS+NYTSG+T   KGV+YSHR AYLNSLA +  +++    SSP +LWT 
Sbjct: 183 EVIRPTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEM---HSSPTYLWTN 242

Query: 241 DMFRCNGWCFIWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPPTLLKMISESSSN 300
            MF CNGWC +W + A+GG NICLR VTA AIF N+  H+VT + G PT+L MI  +  +
Sbjct: 243 PMFHCNGWCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPES 302

Query: 301 NCMPRRLSRRVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPTF--- 360
              P  L  +V  I   A P   ++ K+ ELGF++ + YG+TE  GP  I  WKP +   
Sbjct: 303 EQKP--LPGKVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSL 362

Query: 361 ---EEDNVQFEDLITSL---EIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNMKA 420
              E+  ++    +  L   EI VKDP++M ++  DG T+GEV+ RGNT+M+GY KN +A
Sbjct: 363 PREEQAKMKARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEA 422

Query: 421 THEAFIGENWYRTGDVGVRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPSVA 480
           T EAF G  W+ +GD+GV+H  G IE+KDR+KDI++   G   +S++EVE  L +HP V 
Sbjct: 423 TKEAFKG-GWFWSGDLGVKHPDGYIELKDRSKDIII--SGGENISSIEVESTLFTHPCVL 482

Query: 481 EVAVVGE------RTLYGFVKLKNGGNENGDEIVEFCRMHLPEFMVPKRIVFGDLPMNST 533
           E AVV         T   FVKLK+G   + +E++ +CR  LP +M P+ IVF DLP  ST
Sbjct: 483 EAAVVARPDEYWGETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTST 542

BLAST of Chy6G127960 vs. TAIR 10
Match: AT1G20560.2 (acyl activating enzyme 1 )

HSP 1 Score: 396.7 bits (1018), Expect = 2.9e-110
Identity = 225/480 (46.88%), Postives = 297/480 (61.88%), Query Frame = 0

Query: 78  LYELHFAVPMAGAIISALNTKLDSPTLSLLLQQLNPKVIFLDSQFLPI---LLKSLENNS 137
           + ELHF VPMAGA++  LN + DS  +++LL+    KVIF D QFL I     + L N  
Sbjct: 1   MVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQFLQIAEGACEILSNKG 60

Query: 138 IKFPALVLIPSDPDTPLPS------EFLDYNKVLAMRFGDDDF-TPRPNAELDPISINYT 197
            K P LVLIP +P T   S      E ++Y  V+AM  G  DF   RP  E D IS+NYT
Sbjct: 61  DKVPILVLIP-EPLTQSVSRKKRSEEMMEYEDVVAM--GKSDFEVIRPTDECDAISVNYT 120

Query: 198 SGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAAL 257
           SG+T   KGV+YSHR AYLNSLA +  +++    SSP +LWT  MF CNGWC +W + A+
Sbjct: 121 SGTTSSPKGVVYSHRGAYLNSLAAVLLNEM---HSSPTYLWTNPMFHCNGWCLLWGVTAI 180

Query: 258 GGCNICLRTVTADAIFTNVELHRVTLLCGPPTLLKMISESSSNNCMPRRLSRRVDLIVAG 317
           GG NICLR VTA AIF N+  H+VT + G PT+L MI  +  +   P  L  +V  I   
Sbjct: 181 GGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKP--LPGKVSFITGA 240

Query: 318 ALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPTF------EEDNVQFEDLITSL 377
           A P   ++ K+ ELGF++ + YG+TE  GP  I  WKP +      E+  ++    +  L
Sbjct: 241 APPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMKARQGVNHL 300

Query: 378 ---EIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNMKATHEAFIGENWYRTGDVG 437
              EI VKDP++M ++  DG T+GEV+ RGNT+M+GY KN +AT EAF G  W+ +GD+G
Sbjct: 301 GLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKG-GWFWSGDLG 360

Query: 438 VRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPSVAEVAVVGE------RTLY 497
           V+H  G IE+KDR+KDI++   G   +S++EVE  L +HP V E AVV         T  
Sbjct: 361 VKHPDGYIELKDRSKDIII--SGGENISSIEVESTLFTHPCVLEAAVVARPDEYWGETAC 420

Query: 498 GFVKLKNGGNENGDEIVEFCRMHLPEFMVPKRIVFGDLPMNSTGKVQKFVLREKAKALFS 533
            FVKLK+G   + +E++ +CR  LP +M P+ IVF DLP  STGKVQKFVLR KAKAL S
Sbjct: 421 AFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLRTKAKALVS 469

BLAST of Chy6G127960 vs. TAIR 10
Match: AT1G66120.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 392.9 bits (1008), Expect = 4.2e-109
Identity = 225/561 (40.11%), Postives = 344/561 (61.32%), Query Frame = 0

Query: 1   MEGFYHCPANFGPLSPVPFLKRASTLYGCRPSLVYGTRVFSWSDTYARSLALASALLHHF 60
           M+    C AN  PL+P+ FLKRAS  Y  R S++YG   F+W  TY R   LA++LL   
Sbjct: 1   MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLL-SL 60

Query: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120
           +++  D+V  +APN+P +YE+HF+VPM GA+++ +NT+LD+ T++++L+   PK++F+D 
Sbjct: 61  NITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDY 120

Query: 121 QFLPILLKSLE----NNSIKFPALVLIPSDPDTPLP-SEFLDYNKVLAMRFGDDDFTPRP 180
           +F P++ + L       S   P ++LI     T  P S+ LDY  ++  R G+   TP  
Sbjct: 121 EFAPLIQEVLRLIPTYQSQPHPRIILINEIDSTTKPFSKELDYEGLI--RKGEP--TPSS 180

Query: 181 NA-------ELDPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLW 240
           +A       E DPIS+NYTSG+T   KGV+ SH+ AYL++L++I   ++      PV+LW
Sbjct: 181 SASMFRVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEM---GIFPVYLW 240

Query: 241 TVDMFRCNGWCFIWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPPTLLKMISESS 300
           T+ MF CNGW   W +AA GG N+C+R VTA  I+ N+ELH VT +   PT+ + + E S
Sbjct: 241 TLPMFHCNGWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGS 300

Query: 301 SNNCMPRRLSRRVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPTF- 360
             +  P+  S  V ++  G+ P   ++ KV +LGF++ +GYG+TEA GP +   W+  + 
Sbjct: 301 RTDQSPK--SSPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWN 360

Query: 361 ---EEDNVQFEDL-----ITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNM 420
              E   ++ +       +T  ++DVK+  ++ESV  DG+T+GE++++G++LM GY KN 
Sbjct: 361 KLPEHQQIELQQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNP 420

Query: 421 KATHEAFIGENWYRTGDVGVRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPS 480
           KAT EAF    W  TGD+GV H  G +E+KDR+KDI++   G   +S++EVE VL  +  
Sbjct: 421 KATSEAF-KHGWLNTGDIGVIHPDGYVEIKDRSKDIII--SGGENISSIEVEKVLYMYQE 480

Query: 481 VAEVAVVG-ERTLYG-----FVKLKNGGN---ENGDEIVEFCRMHLPEFMVPKRIV-FGD 531
           V E AVV     L+G     FV LK G      +  +++++CR ++P FM PK++V F +
Sbjct: 481 VLEAAVVAMPHPLWGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQE 540

BLAST of Chy6G127960 vs. TAIR 10
Match: AT1G65890.1 (acyl activating enzyme 12 )

HSP 1 Score: 382.9 bits (982), Expect = 4.3e-106
Identity = 221/578 (38.24%), Postives = 334/578 (57.79%), Query Frame = 0

Query: 1   MEGFYHCPANFGPLSPVPFLKRASTLYGCRPSLVYGTRVFSWSDTYARSLALASALLHHF 60
           M+    C AN  PL+P+ FLKRAS  Y  R S++YG   F+W  TY R   LA++L+   
Sbjct: 1   MDNLALCEANNVPLTPITFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLI-SL 60

Query: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120
           ++   D+V  +APN P +YE+HFAVPMAGA+++ +NT+LD+ +++ +L+   PK++F+  
Sbjct: 61  NIGKNDVVSVVAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFIYR 120

Query: 121 QFLPIL-----LKSLENNSIKFPALVLIPSDPDTPLPSEFLDYN----------KVLAMR 180
            F P+      L S E++++  P + +   D    + SE  DY            +LA  
Sbjct: 121 SFEPLAREILQLLSSEDSNLNLPVIFIHEIDFPKRVSSEESDYECLIQRGEPTPLLLARM 180

Query: 181 FGDDDFTPRPNAELDPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPV 240
           F   D       E DPIS+NYTSG+T   KGV+ SHR AYL++L+ I   ++    + PV
Sbjct: 181 FCIQD-------EHDPISLNYTSGTTADPKGVVISHRGAYLSTLSAIIGWEM---GTCPV 240

Query: 241 FLWTVDMFRCNGWCFIWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPPTLLKMIS 300
           +LWT+ MF CNGW F W  AA GG ++C+R VTA  I+ N+E+H VT +C  PT+  ++ 
Sbjct: 241 YLWTLPMFHCNGWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILL 300

Query: 301 ESSSNNCMPRRLSRRVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKP 360
           + +S +   R  S  V ++  G+ P   ++ KV  LGF + + YG+TEA GP +   W+ 
Sbjct: 301 KGNSLDLSHR--SGPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGLTEATGPVLFCEWQD 360

Query: 361 TFEE--DNVQFE-------DLITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYY 420
            +    +N Q E        ++   E+DV++  + ESV  DG+T+GE++++G+++M GY 
Sbjct: 361 EWNRLPENQQMELKARQGLSILGLTEVDVRNKETQESVPRDGKTMGEIVMKGSSIMKGYL 420

Query: 421 KNMKATHEAFIGENWYRTGDVGVRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMS 480
           KN KAT+EAF    W  +GDVGV H  G +E+KDR+KDI++   G   +S+VEVE ++  
Sbjct: 421 KNPKATYEAF-KHGWLNSGDVGVIHPDGHVEIKDRSKDIII--SGGENISSVEVENIIYK 480

Query: 481 HPSVAEVAVVG------ERTLYGFVKLKNGGNENGD----------EIVEFCRMHLPEFM 538
           +P V E AVV         T   FV L+ G   N D          +++E+CR +LP FM
Sbjct: 481 YPKVLETAVVAMPHPTWGETPCAFVVLEKGETNNEDREDKLVTKERDLIEYCRENLPHFM 540