Chy6G124520 (gene) Cucumber (hystrix) v1

Overview


Name	Chy6G124520
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Subtilisin-like protease
Location	chrH06: 23168004 .. 23169486 (-)
RNA-Seq Expression	Chy6G124520
Synteny	Chy6G124520

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTTCATAACCCATGTAAAGACTGAGTTGGGAATTAAACCATCACCTATGGTGGCTGATTTCTCATCAAGAGGCCCTAATCCCATCATAGACTCCATGATCAAGGTTTGGTTTTAATTCAAACTTTTAACTCAAAAGTCATTTCAAACTCGTACTTATTCAAGTCTTTTCTACTTTATTTGTGTAGCCCGATATTGCAGCGATACGCGTCGGGTGCATTTTAACTTTGATTCAGGTACTTCTAACGTCCACACATCTCAGGTGTTGTTGGCCTTCTCAAGACCCTTTATCCCAAATGGAGCCCTGCAGCCATCAAATCTGCTATCATGACTACAGGTTCATTCAAATGGGTCCATACTTTAATTTATTTATTTTTCGTATTTTATAATTTTGTAATGTTTAAATGTTCGAGCCAACTTATGCACCTCAATTATTCTCTTGGACAATAACTAAATCCTTGATAGGTGACTATCATGGATTGAGATCTTAGCCTTTATCTAATCCAAGTCGACTTTTTATTTACCACTAGATCAACTCACGATAGTTATTTTAGTTTAAATCATCCAAAATAATTTTTATTGGATTTAAGTTAGTCGAAACCCTCTTTAAACATCCACATTTTTCCTAAACTTCTTTAAAAATTAACCATTTTAGTAGTAAACTTTCAAAAATATTTATTTTAGTCTTGGAAACCAGCTCAATTTTTTTCATCTTTGCTCTTGATTTAATGTAATTTGCTTGTAATGAATTCATCGTTAACATGACTTTTAATATGCATAGTTCTTAGATATTATATTAATATGGTAGAACAACTGAGTCGGTAGCAAAAAGATGGATACAACTCATGGTGCATCAAAGTATTATTATTAGTTTGTTATTATGTTTAATAAAATCATTAATGTTTTTTCTAATTCAATCTCCTTTTGTTTCTTTTTCAGCCAAAACAAGAGATAACTCCATGAAGTCAATATTGGACTACAACAAAGTGAAGGCAACCCCATTTCAATATGGTGCAGGACATGTCCATCCAAACAATGCAATTGACCCTGGCCTTGTTTATGACACCACCATTGCAGATTACATGAACTTCATATGTGCTCAAGGCTACAACTCCACCACACTCAAGAAATTCTATAACAAGCCATTCCTTTGCCCCAAATCGTTCGCACTCACAGATCTCAACTACCCCTCAATCTTGGTTCCAAAGCTGACGGCCGGTGTCCCCGTGACGATCAATAGAAGATTTAAGAATGTGGGATTGCCAGGAACGTATGTCGCACAAGTGAAGGTGTCCTCGAAGGTCTCAGTTACTGTCAAGCCGAGTACGTTGCAATTTAATAGTGTGGGTGAAGAGAAGGCTTTCAAGGTTGTATTTGAGTACAAAGGAAAAGGACAAGGTGAAGACCATGTGTTTGGGACATTGATATGGTCAGATGGGAAGCATTTTGTTAGAAGTCCTATGGCTGTGAAATTGGCATGA

mRNA sequence

ATGGCTTTCATAACCCATGTAAAGACTGAGTTGGGAATTAAACCATCACCTATGGTGGCTGATTTCTCATCAAGAGGCCCTAATCCCATCATAGACTCCATGATCAAGCCCGATATTGCAGCGATACGCGTCGGGTGCATTTTAACTTTGATTCAGACCCTTTATCCCAAATGGAGCCCTGCAGCCATCAAATCTGCTATCATGACTACAGCCAAAACAAGAGATAACTCCATGAAGTCAATATTGGACTACAACAAAGTGAAGGCAACCCCATTTCAATATGGTGCAGGACATGTCCATCCAAACAATGCAATTGACCCTGGCCTTGTTTATGACACCACCATTGCAGATTACATGAACTTCATATGTGCTCAAGGCTACAACTCCACCACACTCAAGAAATTCTATAACAAGCCATTCCTTTGCCCCAAATCGTTCGCACTCACAGATCTCAACTACCCCTCAATCTTGGTTCCAAAGCTGACGGCCGGTGTCCCCGTGACGATCAATAGAAGATTTAAGAATGTGGGATTGCCAGGAACGTATGTCGCACAAGTGAAGGTGTCCTCGAAGGTCTCAGTTACTGTCAAGCCGAGTACGTTGCAATTTAATAGTGTGGGTGAAGAGAAGGCTTTCAAGGTTGTATTTGAGTACAAAGGAAAAGGACAAGGTGAAGACCATGTGTTTGGGACATTGATATGGTCAGATGGGAAGCATTTTGTTAGAAGTCCTATGGCTGTGAAATTGGCATGA

Coding sequence (CDS)

ATGGCTTTCATAACCCATGTAAAGACTGAGTTGGGAATTAAACCATCACCTATGGTGGCTGATTTCTCATCAAGAGGCCCTAATCCCATCATAGACTCCATGATCAAGCCCGATATTGCAGCGATACGCGTCGGGTGCATTTTAACTTTGATTCAGACCCTTTATCCCAAATGGAGCCCTGCAGCCATCAAATCTGCTATCATGACTACAGCCAAAACAAGAGATAACTCCATGAAGTCAATATTGGACTACAACAAAGTGAAGGCAACCCCATTTCAATATGGTGCAGGACATGTCCATCCAAACAATGCAATTGACCCTGGCCTTGTTTATGACACCACCATTGCAGATTACATGAACTTCATATGTGCTCAAGGCTACAACTCCACCACACTCAAGAAATTCTATAACAAGCCATTCCTTTGCCCCAAATCGTTCGCACTCACAGATCTCAACTACCCCTCAATCTTGGTTCCAAAGCTGACGGCCGGTGTCCCCGTGACGATCAATAGAAGATTTAAGAATGTGGGATTGCCAGGAACGTATGTCGCACAAGTGAAGGTGTCCTCGAAGGTCTCAGTTACTGTCAAGCCGAGTACGTTGCAATTTAATAGTGTGGGTGAAGAGAAGGCTTTCAAGGTTGTATTTGAGTACAAAGGAAAAGGACAAGGTGAAGACCATGTGTTTGGGACATTGATATGGTCAGATGGGAAGCATTTTGTTAGAAGTCCTATGGCTGTGAAATTGGCATGA

Protein sequence

MAFITHVKTELGIKPSPMVADFSSRGPNPIIDSMIKPDIAAIRVGCILTLIQTLYPKWSPAAIKSAIMTTAKTRDNSMKSILDYNKVKATPFQYGAGHVHPNNAIDPGLVYDTTIADYMNFICAQGYNSTTLKKFYNKPFLCPKSFALTDLNYPSILVPKLTAGVPVTINRRFKNVGLPGTYVAQVKVSSKVSVTVKPSTLQFNSVGEEKAFKVVFEYKGKGQGEDHVFGTLIWSDGKHFVRSPMAVKLA*

Homology

[Close]

BLAST of Chy6G124520 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 2.2e-57
Identity = 126/288 (43.75%), Postives = 167/288 (57.99%), Query Frame = 0


Query: 1   MAFITHVKTELGIKPSPMVADFSSRGPNPIIDSMIKPDIAAIRVGCILT----------- 60
           +A IT  +T+LG+KP+P++A FSS+GP+ +   ++KPDI A  V  I             
Sbjct: 487 IAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQ 546

Query: 61  --------------------------LIQTLYPKWSPAAIKSAIMTTAKTRDNSMKSILD 120
                                     L++T YP WSPAAI+SAIMTTA   D+    I +
Sbjct: 547 FDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQN 606

Query: 121 YNKVKATPFQYGAGHVHPNNAIDPGLVYDTTIADYMNFICAQGYNSTTLKKFYNKPFLCP 180
              +KATPF +GAGHV PN A++PGLVYD  I DY+NF+C+ GYN++ +  F    F C 
Sbjct: 607 ATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCS 666

Query: 181 K-SFALTDLNYPSILVPKLTAGVPVTINRRFKNVGLPGTYVAQVKVSSKVSVTVKPSTLQ 240
               +L +LNYPSI VP LT+   VT++R  KNVG P  Y  +V     V V VKP++L 
Sbjct: 667 SPKISLVNLNYPSITVPNLTSS-KVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLN 726

Query: 241 FNSVGEEKAFKVVFEYKGKGQ-GEDHVFGTLIWSDGKHFVRSPMAVKL 250
           F  VGE+K FKV+   K KG   + +VFG L+WSD KH VRSP+ VKL
Sbjct: 727 FTKVGEQKTFKVIL-VKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

[Close]

BLAST of Chy6G124520 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 211.8 bits (538), Expect = 8.7e-54
Identity = 117/285 (41.05%), Postives = 158/285 (55.44%), Query Frame = 0


Query: 4   ITHVKTELGIKPSPMVADFSSRGPNPIIDSMIKPDIAAIRVGC----------------- 63
           ++  +T  G KP+P++A FSSRGPN I  S++KPD+ A  V                   
Sbjct: 504 MSRARTLFGRKPAPVMASFSSRGPNKIQPSILKPDVTAPGVNILAAYSEFASASSLLVDN 563

Query: 64  ---------------------ILTLIQTLYPKWSPAAIKSAIMTTAKTRDNSMKSILD-Y 123
                                I  L++T +P WSPAAIKSAIMTTA T DN+ + I D +
Sbjct: 564 RRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPAAIKSAIMTTATTLDNTNRPIQDAF 623

Query: 124 NKVKATPFQYGAGHVHPNNAIDPGLVYDTTIADYMNFICAQGYNSTTLKKF-YNKPFLCP 183
           +K  A  F YG+GHV P+ AI+PGLVYD ++ DY+NF+CA GY+   +    +N+ F+C 
Sbjct: 624 DKTLADAFAYGSGHVRPDLAIEPGLVYDLSLTDYLNFLCASGYDQQLISALNFNRTFICS 683

Query: 184 KSFALTDLNYPSILVPKLTAGVPVTINRRFKNVGLPGTYVAQVKVSSKVSVTVKPSTLQF 243
            S ++ DLNYPSI +P L    PVTI R   NVG P TY    +  +  S+ V P +L F
Sbjct: 684 GSHSVNDLNYPSITLPNLRL-KPVTIARTVTNVGPPSTYTVSTRSPNGYSIAVVPPSLTF 743

Query: 244 NSVGEEKAFKVVFEYKGKGQGEDHVFGTLIWSDGKHFVRSPMAVK 249
             +GE K FKV+ +         + FG L W+DGKH VRSP+ VK
Sbjct: 744 TKIGERKTFKVIVQASSAATRRKYEFGDLRWTDGKHIVRSPITVK 787

[Close]

BLAST of Chy6G124520 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 4.3e-53
Identity = 117/278 (42.09%), Postives = 156/278 (56.12%), Query Frame = 0


Query: 11  LGIKPSPMVADFSSRGPNPIIDSMIKPDIAAIRVGCI----------------------- 70
           L  KP+P +A FSSRGPN I   ++KPDI A  V  I                       
Sbjct: 503 LNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNT 562

Query: 71  --------------LTLIQTLYPKWSPAAIKSAIMTTAKTRDNSMKSILDYNKVKATPFQ 130
                         + L++TL+P WSPAAI+SAIMTT++TR+N  K ++D +  KA PF 
Sbjct: 563 ESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFKKANPFS 622

Query: 131 YGAGHVHPNNAIDPGLVYDTTIADYMNFICAQGYNSTTLKKFYNKP-FLCPKSFALTDLN 190
           YG+GHV PN A  PGLVYD T  DY++F+CA GYN+T ++ F   P + C +   L D N
Sbjct: 623 YGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGANLLDFN 682

Query: 191 YPSILVPKLTAGVPVTINRRFKNVGLPGTYVAQVKVSSKVSVTVKPSTLQFNSVGEEKAF 250
           YPSI VP LT    +T+ R+ KNVG P TY A+ +    V V+V+P  L FN  GE K F
Sbjct: 683 YPSITVPNLTGS--ITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEVKIF 742

[Close]

BLAST of Chy6G124520 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 1.5e-42
Identity = 106/289 (36.68%), Postives = 153/289 (52.94%), Query Frame = 0


Query: 2   AFITHVKTELGIKPSPMVADFSSRGPNPIIDSMIKPDIAA-------------------- 61
           A I+ + T +G+KPSP+VA FSSRGPN I  +++KPD+ A                    
Sbjct: 469 ASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLAS 528

Query: 62  -----------------IRVGCILTLIQTLYPKWSPAAIKSAIMTTAKTRDNSMKSILDY 121
                              V  +  L+++++P+WSPAAI+SA+MTTA       K +LD 
Sbjct: 529 DSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDI 588

Query: 122 NKVK-ATPFQYGAGHVHPNNAIDPGLVYDTTIADYMNFICAQGYNSTTLKKFYNKPFLC- 181
              K +TPF +GAGHV P  A +PGL+YD T  DY+ F+CA  Y S  ++    + + C 
Sbjct: 589 ATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCD 648

Query: 182 -PKSFALTDLNYPSILVPKLTAGVPVTINRRFKNVGLPGTYVAQVKVSSK---VSVTVKP 241
             KS+++ DLNYPS  V     G      R   +VG  GTY   VKV+S+   V ++V+P
Sbjct: 649 PSKSYSVADLNYPSFAVNVDGVGA-YKYTRTVTSVGGAGTY--SVKVTSETTGVKISVEP 708

Query: 242 STLQFNSVGEEKAFKVVFEYKGKGQGEDHVFGTLIWSDGKHFVRSPMAV 248
           + L F    E+K++ V F          + FG++ WSDGKH V SP+A+
Sbjct: 709 AVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAI 754

[Close]

BLAST of Chy6G124520 vs. ExPASy Swiss-Prot
Match: F4JJL8 (Subtilisin-like protease SBT3.15 OS=Arabidopsis thaliana OX=3702 GN=SBT3.15 PE=2 SV=2)

HSP 1 Score: 153.3 bits (386), Expect = 3.7e-36
Identity = 100/282 (35.46%), Postives = 144/282 (51.06%), Query Frame = 0


Query: 4   ITHVKTELGIKPSPMVADFSSRGPNPIIDSMIKPDIAAIRVGC----------------- 63
           I+   T  G   +P VA FSSRGPN +  +++KPDIAA  V                   
Sbjct: 480 ISAATTLTGQPATPKVAAFSSRGPNSVSPAILKPDIAAPGVSILAAVSPLDPGAFNGFKL 539

Query: 64  -------------ILTLIQTLYPKWSPAAIKSAIMTTAKTRDNSMKSIL--DYNKVKATP 123
                        I+ L+++L+PKWSPAA++SA++TTA     S + I     NK  A P
Sbjct: 540 HSGTSMSTPVVSGIIVLLKSLHPKWSPAAMRSALVTTAWRTSPSGEPIFAQGSNKKLADP 599

Query: 124 FQYGAGHVHPNNAIDPGLVYDTTIADYMNFICAQGYNSTTLKKFYNKPFLCP-KSFALTD 183
           F YG G V+P  A  PGLVYD  I DY+N++C+ GYN +++ +   K   CP    ++ D
Sbjct: 600 FDYGGGLVNPEKAAKPGLVYDMGIKDYINYMCSAGYNDSSISRVLGKKTKCPIPKPSMLD 659

Query: 184 LNYPSILVPKLTAGVPVTINRRFKNVG-LPGTYVAQVKVSSKVSVTVKPSTLQFNSVGEE 243
           +N PSI +P L     VT+ R   NVG +   Y A ++    +++TV P+ L F S  + 
Sbjct: 660 INLPSITIPNLEK--EVTLTRTVTNVGPIKSVYRAVIESPLGITLTVNPTILVFKSAAKR 719

Query: 244 KAFKVVFEYKGKGQ---GEDHVFGTLIWSDGKHFVRSPMAVK 249
               + F  K K        + FG+L W+DG H V  P++VK
Sbjct: 720 ---VLTFSVKAKTSHKVNSGYFFGSLTWTDGVHDVTIPVSVK 756

[Close]

BLAST of Chy6G124520 vs. ExPASy TrEMBL
Match: A0A5D3D732 (Subtilisin-like protease SBT5.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00170 PE=3 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 2.2e-121
Identity = 227/286 (79.37%), Postives = 235/286 (82.17%), Query Frame = 0


Query: 1    MAFITHVKTELGIKPSPMVADFSSRGPNPIIDSMIKPDIAAIRVGC-------------- 60
            MAFITHVKTELGIKPSPMVADFSSRGPNPIIDSMIKPDIAA  V                
Sbjct: 1196 MAFITHVKTELGIKPSPMVADFSSRGPNPIIDSMIKPDIAAPGVSILAAFSEYATATDFP 1255

Query: 61   -----------------------ILTLIQTLYPKWSPAAIKSAIMTTAKTRDNSMKSILD 120
                                   ++ L++TLYPKWSPAAIKSAIMTTAKTRDNSMKSILD
Sbjct: 1256 LDTRRVSFNFESGTSMACPHISGVVGLLKTLYPKWSPAAIKSAIMTTAKTRDNSMKSILD 1315

Query: 121  YNKVKATPFQYGAGHVHPNNAIDPGLVYDTTIADYMNFICAQGYNSTTLKKFYNKPFLCP 180
            YNK KATPFQYGAGHVHPNNAIDPGLVYDTTI DYMNFICAQGYNSTTLK+FYNKP+LCP
Sbjct: 1316 YNKAKATPFQYGAGHVHPNNAIDPGLVYDTTIEDYMNFICAQGYNSTTLKRFYNKPYLCP 1375

Query: 181  KSFALTDLNYPSILVPKLTAGVPVTINRRFKNVGLPGTYVAQVKVSSKVSVTVKPSTLQF 240
            KSF LTDLNYPSI VPKLT GVPVTINRR KNVG PGTYVA+VKVSSKVSVTVKPSTLQF
Sbjct: 1376 KSFPLTDLNYPSISVPKLTIGVPVTINRRLKNVGTPGTYVARVKVSSKVSVTVKPSTLQF 1435

Query: 241  NSVGEEKAFKVVFEYKGKGQGEDHVFGTLIWSDGKHFVRSPMAVKL 250
            NSVGEEKAFKVVFEYKGKGQG+ HVFGTLIWSDGKHFVRSPM VKL
Sbjct: 1436 NSVGEEKAFKVVFEYKGKGQGKGHVFGTLIWSDGKHFVRSPMVVKL 1481

[Close]

BLAST of Chy6G124520 vs. ExPASy TrEMBL
Match: A0A1S3BLG8 (subtilisin-like protease SBT5.3 OS=Cucumis melo OX=3656 GN=LOC103491134 PE=3 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 5.0e-121
Identity = 226/286 (79.02%), Postives = 235/286 (82.17%), Query Frame = 0


Query: 1    MAFITHVKTELGIKPSPMVADFSSRGPNPIIDSMIKPDIAAIRVGC-------------- 60
            MAFITHVKTE+GIKPSPMVADFSSRGPNPIIDSMIKPDIAA  V                
Sbjct: 1225 MAFITHVKTEMGIKPSPMVADFSSRGPNPIIDSMIKPDIAAPGVSILAAFSEYATATDFP 1284

Query: 61   -----------------------ILTLIQTLYPKWSPAAIKSAIMTTAKTRDNSMKSILD 120
                                   ++ L++TLYPKWSPAAIKSAIMTTAKTRDNSMKSILD
Sbjct: 1285 LDTRRVSFNFESGTSMACPHISGVVGLLKTLYPKWSPAAIKSAIMTTAKTRDNSMKSILD 1344

Query: 121  YNKVKATPFQYGAGHVHPNNAIDPGLVYDTTIADYMNFICAQGYNSTTLKKFYNKPFLCP 180
            YNK KATPFQYGAGHVHPNNAIDPGLVYDTTI DYMNFICAQGYNSTTLK+FYNKP+LCP
Sbjct: 1345 YNKAKATPFQYGAGHVHPNNAIDPGLVYDTTIEDYMNFICAQGYNSTTLKRFYNKPYLCP 1404

Query: 181  KSFALTDLNYPSILVPKLTAGVPVTINRRFKNVGLPGTYVAQVKVSSKVSVTVKPSTLQF 240
            KSF LTDLNYPSI VPKLT GVPVTINRR KNVG PGTYVA+VKVSSKVSVTVKPSTLQF
Sbjct: 1405 KSFPLTDLNYPSISVPKLTIGVPVTINRRLKNVGTPGTYVARVKVSSKVSVTVKPSTLQF 1464

Query: 241  NSVGEEKAFKVVFEYKGKGQGEDHVFGTLIWSDGKHFVRSPMAVKL 250
            NSVGEEKAFKVVFEYKGKGQG+ HVFGTLIWSDG HFVRSPMAVKL
Sbjct: 1465 NSVGEEKAFKVVFEYKGKGQGKGHVFGTLIWSDGNHFVRSPMAVKL 1510

[Close]

BLAST of Chy6G124520 vs. ExPASy TrEMBL
Match: A0A0A0L601 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045060 PE=3 SV=1)

HSP 1 Score: 360.9 bits (925), Expect = 4.3e-96
Identity = 181/286 (63.29%), Postives = 212/286 (74.13%), Query Frame = 0


Query: 1   MAFITHVKTELGIKPSPMVADFSSRGPNPIIDSMIKPDI--------------------- 60
           +A IT V+T+LGIKPSP++ADFSSRGPNPI ++M+KPDI                     
Sbjct: 460 VAHITPVQTQLGIKPSPVMADFSSRGPNPITEAMLKPDITGPGMSILASVTTDVTATTFP 519

Query: 61  ----------------AAIRVGCILTLIQTLYPKWSPAAIKSAIMTTAKTRDNSMKSILD 120
                           +   +  ++ L++TLYP WSPAAIKSAIMTTAKTRDN+M++I D
Sbjct: 520 FDTRRVPFNVESGTSMSCPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMRTISD 579

Query: 121 YNKVKATPFQYGAGHVHPNNAIDPGLVYDTTIADYMNFICAQGYNSTTLKKFYNKPFLCP 180
             K KATPF YGAGHVHPN+A+DPGLVYDTTI DY+NF+CA+GYNS T K FYNKPF+C 
Sbjct: 580 NVKPKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTFKNFYNKPFVCA 639

Query: 181 KSFALTDLNYPSILVPKLTAGVPVTINRRFKNVGLPGTYVAQVKVSSKVSVTVKPSTLQF 240
           KSF LTDLNYPSI +PKL  G PVT+NRR KNVG PGTYVA+V  SSK+ VTV+PSTLQF
Sbjct: 640 KSFTLTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVEPSTLQF 699

Query: 241 NSVGEEKAFKVVFEYKGKGQGEDHVFGTLIWSDGKHFVRSPMAVKL 250
           NSVGEEKAFKVVFEYKG  Q + +VFGTLIWSDGKH VRSP+ V L
Sbjct: 700 NSVGEEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNL 745

[Close]

BLAST of Chy6G124520 vs. ExPASy TrEMBL
Match: A0A6J1KUL8 (uncharacterized protein LOC111498820 OS=Cucurbita maxima OX=3661 GN=LOC111498820 PE=3 SV=1)

HSP 1 Score: 341.7 bits (875), Expect = 2.7e-90
Identity = 174/286 (60.84%), Postives = 209/286 (73.08%), Query Frame = 0


Query: 1   MAFITHVKTELGIKPSPMVADFSSRGPNPIIDSMIKPDIAAIRVGCILT----------- 60
           +A IT V+TE+GIKPSP++A FSSRGPN I ++MIKPDI+A  V  I +           
Sbjct: 475 VASITPVRTEIGIKPSPVMAPFSSRGPNHIAEAMIKPDISAPGVNIIASFTKAVAATDLP 534

Query: 61  --------------------------LIQTLYPKWSPAAIKSAIMTTAKTRDNSMKSILD 120
                                     L++ L+P WSPAAIKSAIMTTAKTRDN+  ++LD
Sbjct: 535 FDKRRVPFNVQSGTSMSCPHIAGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLD 594

Query: 121 YNKVKATPFQYGAGHVHPNNAIDPGLVYDTTIADYMNFICAQGYNSTTLKKFYNKPFLCP 180
           +NKVKATPF YGAGHVHPN+A+DPGLVYDTTI DY+NF+C QGYNS TLKKF NKPF+C 
Sbjct: 595 FNKVKATPFDYGAGHVHPNDAMDPGLVYDTTIDDYLNFLCMQGYNSLTLKKFSNKPFVCA 654

Query: 181 KSFALTDLNYPSILVPKLTAGVPVTINRRFKNVGLPGTYVAQVKVSSKVSVTVKPSTLQF 240
           K+FA TDLNYPSI VPKL  GVPVT+NRR KNVG  GTYVA+V++   ++V V+PSTLQF
Sbjct: 655 KNFASTDLNYPSISVPKLQIGVPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQF 714

Query: 241 NSVGEEKAFKVVFEYKGKGQGEDHVFGTLIWSDGKHFVRSPMAVKL 250
           +SVGEEKAFK+VF Y  K +   +VFG L+WSDGKHFVRSP+AV L
Sbjct: 715 HSVGEEKAFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNL 760

[Close]

BLAST of Chy6G124520 vs. ExPASy TrEMBL
Match: A0A6J1GDR2 (subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111453063 PE=3 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 1.2e-87
Identity = 169/286 (59.09%), Postives = 205/286 (71.68%), Query Frame = 0


Query: 1   MAFITHVKTELGIKPSPMVADFSSRGPNPIIDSMIKPDIAAIRVGCILT----------- 60
           +A IT V+TE+GIKPSP++A FSSRGPN I +++IKPDI A  V  I +           
Sbjct: 480 VATITPVRTEIGIKPSPVMATFSSRGPNHITEAIIKPDITAPGVNIIASFTRAAAATDLP 539

Query: 61  --------------------------LIQTLYPKWSPAAIKSAIMTTAKTRDNSMKSILD 120
                                     L++ L+P WSPAAIKSAIMTTAKTRDN+  ++LD
Sbjct: 540 FDKRRVPFNVQSGTSMSCPHISGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLD 599

Query: 121 YNKVKATPFQYGAGHVHPNNAIDPGLVYDTTIADYMNFICAQGYNSTTLKKFYNKPFLCP 180
           +NKVKATPF YGAG VHPNNA+DPGLVYDTTI DY+NF+C QGYNS TLKKF NKPF+C 
Sbjct: 600 FNKVKATPFDYGAGLVHPNNAMDPGLVYDTTIDDYLNFLCTQGYNSLTLKKFSNKPFVCS 659

Query: 181 KSFALTDLNYPSILVPKLTAGVPVTINRRFKNVGLPGTYVAQVKVSSKVSVTVKPSTLQF 240
           K+FA+TDLNYPSI VPKL  G PVT+NRR KNVG  GTYVA+V++   ++V V+PSTLQF
Sbjct: 660 KNFAITDLNYPSISVPKLQIGAPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQF 719

Query: 241 NSVGEEKAFKVVFEYKGKGQGEDHVFGTLIWSDGKHFVRSPMAVKL 250
           + VGEEK FK+VF Y  + +   +VFG L+WSDGKHFVRSP+AV L
Sbjct: 720 HGVGEEKPFKLVFHYAQQVRRPGYVFGALVWSDGKHFVRSPIAVNL 765

[Close]

BLAST of Chy6G124520 vs. NCBI nr
Match: TYK19386.1 (subtilisin-like protease SBT5.3 [Cucumis melo var. makuwa])

HSP 1 Score: 444 bits (1143), Expect = 1.73e-142
Identity = 227/286 (79.37%), Postives = 235/286 (82.17%), Query Frame = 0


Query: 1    MAFITHVKTELGIKPSPMVADFSSRGPNPIIDSMIKPDIAAIRVGC-------------- 60
            MAFITHVKTELGIKPSPMVADFSSRGPNPIIDSMIKPDIAA  V                
Sbjct: 1196 MAFITHVKTELGIKPSPMVADFSSRGPNPIIDSMIKPDIAAPGVSILAAFSEYATATDFP 1255

Query: 61   -----------------------ILTLIQTLYPKWSPAAIKSAIMTTAKTRDNSMKSILD 120
                                   ++ L++TLYPKWSPAAIKSAIMTTAKTRDNSMKSILD
Sbjct: 1256 LDTRRVSFNFESGTSMACPHISGVVGLLKTLYPKWSPAAIKSAIMTTAKTRDNSMKSILD 1315

Query: 121  YNKVKATPFQYGAGHVHPNNAIDPGLVYDTTIADYMNFICAQGYNSTTLKKFYNKPFLCP 180
            YNK KATPFQYGAGHVHPNNAIDPGLVYDTTI DYMNFICAQGYNSTTLK+FYNKP+LCP
Sbjct: 1316 YNKAKATPFQYGAGHVHPNNAIDPGLVYDTTIEDYMNFICAQGYNSTTLKRFYNKPYLCP 1375

Query: 181  KSFALTDLNYPSILVPKLTAGVPVTINRRFKNVGLPGTYVAQVKVSSKVSVTVKPSTLQF 240
            KSF LTDLNYPSI VPKLT GVPVTINRR KNVG PGTYVA+VKVSSKVSVTVKPSTLQF
Sbjct: 1376 KSFPLTDLNYPSISVPKLTIGVPVTINRRLKNVGTPGTYVARVKVSSKVSVTVKPSTLQF 1435

Query: 241  NSVGEEKAFKVVFEYKGKGQGEDHVFGTLIWSDGKHFVRSPMAVKL 249
            NSVGEEKAFKVVFEYKGKGQG+ HVFGTLIWSDGKHFVRSPM VKL
Sbjct: 1436 NSVGEEKAFKVVFEYKGKGQGKGHVFGTLIWSDGKHFVRSPMVVKL 1481

[Close]

BLAST of Chy6G124520 vs. NCBI nr
Match: XP_008449184.1 (PREDICTED: subtilisin-like protease SBT5.3 [Cucumis melo])

HSP 1 Score: 443 bits (1140), Expect = 7.08e-142
Identity = 226/286 (79.02%), Postives = 235/286 (82.17%), Query Frame = 0


Query: 1    MAFITHVKTELGIKPSPMVADFSSRGPNPIIDSMIKPDIAAIRVGC-------------- 60
            MAFITHVKTE+GIKPSPMVADFSSRGPNPIIDSMIKPDIAA  V                
Sbjct: 1225 MAFITHVKTEMGIKPSPMVADFSSRGPNPIIDSMIKPDIAAPGVSILAAFSEYATATDFP 1284

Query: 61   -----------------------ILTLIQTLYPKWSPAAIKSAIMTTAKTRDNSMKSILD 120
                                   ++ L++TLYPKWSPAAIKSAIMTTAKTRDNSMKSILD
Sbjct: 1285 LDTRRVSFNFESGTSMACPHISGVVGLLKTLYPKWSPAAIKSAIMTTAKTRDNSMKSILD 1344

Query: 121  YNKVKATPFQYGAGHVHPNNAIDPGLVYDTTIADYMNFICAQGYNSTTLKKFYNKPFLCP 180
            YNK KATPFQYGAGHVHPNNAIDPGLVYDTTI DYMNFICAQGYNSTTLK+FYNKP+LCP
Sbjct: 1345 YNKAKATPFQYGAGHVHPNNAIDPGLVYDTTIEDYMNFICAQGYNSTTLKRFYNKPYLCP 1404

Query: 181  KSFALTDLNYPSILVPKLTAGVPVTINRRFKNVGLPGTYVAQVKVSSKVSVTVKPSTLQF 240
            KSF LTDLNYPSI VPKLT GVPVTINRR KNVG PGTYVA+VKVSSKVSVTVKPSTLQF
Sbjct: 1405 KSFPLTDLNYPSISVPKLTIGVPVTINRRLKNVGTPGTYVARVKVSSKVSVTVKPSTLQF 1464

Query: 241  NSVGEEKAFKVVFEYKGKGQGEDHVFGTLIWSDGKHFVRSPMAVKL 249
            NSVGEEKAFKVVFEYKGKGQG+ HVFGTLIWSDG HFVRSPMAVKL
Sbjct: 1465 NSVGEEKAFKVVFEYKGKGQGKGHVFGTLIWSDGNHFVRSPMAVKL 1510

[Close]

BLAST of Chy6G124520 vs. NCBI nr
Match: XP_038905648.1 (subtilisin-like protease SBT5.4 [Benincasa hispida])

HSP 1 Score: 403 bits (1036), Expect = 4.01e-133
Identity = 203/287 (70.73%), Postives = 229/287 (79.79%), Query Frame = 0


Query: 1   MAFITHVKTELGIKPSPMVADFSSRGPNPIIDSMIKPDIAA------------------- 60
           +A I+HV+TELGIKPSPM+ DFSSRGPNPIIDSMIKPDI A                   
Sbjct: 475 VASISHVETELGIKPSPMMGDFSSRGPNPIIDSMIKPDITAPGLNIIAAVSESATATSSA 534

Query: 61  -------------IRVGC-----ILTLIQTLYPKWSPAAIKSAIMTTAKTRDNSMKSILD 120
                          + C     ++ L++TLYPKWSPAAI+SAIMTTAKTRDNS KSILD
Sbjct: 535 YDTRRVPFNFEIGTSMSCPHISGVVGLLKTLYPKWSPAAIRSAIMTTAKTRDNSKKSILD 594

Query: 121 YNKVKATPFQYGAGHVHPNNAIDPGLVYDTTIADYMNFICAQGYNSTTLKKFYNKPFLCP 180
           YNK+KATPF YGAGHVHPNNA+DPGLVYDTT  DYMNF+CA GYN TTL++FYNKP++CP
Sbjct: 595 YNKMKATPFHYGAGHVHPNNAMDPGLVYDTTTEDYMNFLCALGYNYTTLREFYNKPYVCP 654

Query: 181 KSFALTDLNYPSILVPKLTAGVPVTINRRFKNVGLPGTYVAQVKVSSKVSVTVKPSTLQF 240
           +SFA+ DLNYPSI VPKLTAGVPVTINR+ KNVG PGTYVA+VKVSS VSVTV+PSTLQF
Sbjct: 655 ESFAVMDLNYPSISVPKLTAGVPVTINRKVKNVGSPGTYVARVKVSSMVSVTVEPSTLQF 714

Query: 241 NSVGEEKAFKVVFEYKGKGQGEDHVFGTLIWSDGKHFVRSPMAVKLA 250
           +S GEEKAFKVVF+YKG GQG+DHVFGTLIWSDGKHFVRSPMA+ LA
Sbjct: 715 DSEGEEKAFKVVFQYKGTGQGKDHVFGTLIWSDGKHFVRSPMAMALA 761

[Close]

BLAST of Chy6G124520 vs. NCBI nr
Match: XP_038905686.1 (subtilisin-like protease SBT5.3 [Benincasa hispida])

HSP 1 Score: 377 bits (968), Expect = 7.04e-123
Identity = 188/286 (65.73%), Postives = 219/286 (76.57%), Query Frame = 0


Query: 1   MAFITHVKTELGIKPSPMVADFSSRGPNPIIDSMIKPDIAAIRVGCILT----------- 60
           +A+ITHVKTE+GIKPSP++ADFSSRGPN I ++M+KPDI A  V  I +           
Sbjct: 480 VAYITHVKTEVGIKPSPVMADFSSRGPNSITEAMLKPDITAPGVNIIASVTIDATATDSP 539

Query: 61  --------------------------LIQTLYPKWSPAAIKSAIMTTAKTRDNSMKSILD 120
                                     L++TLYP WSPAAIKSAIMTTAKTRDN+ ++I D
Sbjct: 540 FDTRRVPFNVESGTSMSCPHISGVAGLLKTLYPTWSPAAIKSAIMTTAKTRDNTQRTISD 599

Query: 121 YNKVKATPFQYGAGHVHPNNAIDPGLVYDTTIADYMNFICAQGYNSTTLKKFYNKPFLCP 180
             K+KATPF YGAGHVHPN+A+DPGLVYDTTI DY+NF+CA+GYNS  LKKFYNKPF+C 
Sbjct: 600 TAKMKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSVALKKFYNKPFICA 659

Query: 181 KSFALTDLNYPSILVPKLTAGVPVTINRRFKNVGLPGTYVAQVKVSSKVSVTVKPSTLQF 240
           KSFA+TDLNYPSI VP+L  G PVT+NRR KNVG PGTYVA+VK S  +SV+V+PSTLQF
Sbjct: 660 KSFAITDLNYPSISVPELRIGAPVTVNRRVKNVGTPGTYVARVKASPAISVSVEPSTLQF 719

Query: 241 NSVGEEKAFKVVFEYKGKGQGEDHVFGTLIWSDGKHFVRSPMAVKL 249
           NSVGEEKAFKVVF+YKGK Q + HVFGTLIWSDGKHFVRSP+AVKL
Sbjct: 720 NSVGEEKAFKVVFQYKGKEQRQGHVFGTLIWSDGKHFVRSPIAVKL 765

[Close]

BLAST of Chy6G124520 vs. NCBI nr
Match: KAE8650093.1 (hypothetical protein Csa_009608 [Cucumis sativus])

HSP 1 Score: 360 bits (925), Expect = 7.40e-116
Identity = 182/286 (63.64%), Postives = 213/286 (74.48%), Query Frame = 0


Query: 1   MAFITHVKTELGIKPSPMVADFSSRGPNPIIDSMIKPDI--------------------- 60
           +A IT V+T+LGIKPSP++ADFSSRGPNPI ++M+KPDI                     
Sbjct: 537 VAHITPVQTQLGIKPSPVMADFSSRGPNPITEAMLKPDITGPGMSILASVTTDVTATTFP 596

Query: 61  -----------AAIRVGC-----ILTLIQTLYPKWSPAAIKSAIMTTAKTRDNSMKSILD 120
                      +   + C     ++ L++TLYP WSPAAIKSAIMTTAKTRDN+M++I D
Sbjct: 597 FDTRRVPFNVESGTSMSCPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMRTISD 656

Query: 121 YNKVKATPFQYGAGHVHPNNAIDPGLVYDTTIADYMNFICAQGYNSTTLKKFYNKPFLCP 180
             K KATPF YGAGHVHPN+A+DPGLVYDTTI DY+NF+CA+GYNS T K FYNKPF+C 
Sbjct: 657 NVKPKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTFKNFYNKPFVCA 716

Query: 181 KSFALTDLNYPSILVPKLTAGVPVTINRRFKNVGLPGTYVAQVKVSSKVSVTVKPSTLQF 240
           KSF LTDLNYPSI +PKL  G PVT+NRR KNVG PGTYVA+V  SSK+ VTV+PSTLQF
Sbjct: 717 KSFTLTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVEPSTLQF 776

Query: 241 NSVGEEKAFKVVFEYKGKGQGEDHVFGTLIWSDGKHFVRSPMAVKL 249
           NSVGEEKAFKVVFEYKG  Q + +VFGTLIWSDGKH VRSP+ V L
Sbjct: 777 NSVGEEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNL 822

[Close]

BLAST of Chy6G124520 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 223.8 bits (569), Expect = 1.6e-58
Identity = 126/288 (43.75%), Postives = 167/288 (57.99%), Query Frame = 0


Query: 1   MAFITHVKTELGIKPSPMVADFSSRGPNPIIDSMIKPDIAAIRVGCILT----------- 60
           +A IT  +T+LG+KP+P++A FSS+GP+ +   ++KPDI A  V  I             
Sbjct: 487 IAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQ 546

Query: 61  --------------------------LIQTLYPKWSPAAIKSAIMTTAKTRDNSMKSILD 120
                                     L++T YP WSPAAI+SAIMTTA   D+    I +
Sbjct: 547 FDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQN 606

Query: 121 YNKVKATPFQYGAGHVHPNNAIDPGLVYDTTIADYMNFICAQGYNSTTLKKFYNKPFLCP 180
              +KATPF +GAGHV PN A++PGLVYD  I DY+NF+C+ GYN++ +  F    F C 
Sbjct: 607 ATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCS 666

Query: 181 K-SFALTDLNYPSILVPKLTAGVPVTINRRFKNVGLPGTYVAQVKVSSKVSVTVKPSTLQ 240
               +L +LNYPSI VP LT+   VT++R  KNVG P  Y  +V     V V VKP++L 
Sbjct: 667 SPKISLVNLNYPSITVPNLTSS-KVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLN 726

Query: 241 FNSVGEEKAFKVVFEYKGKGQ-GEDHVFGTLIWSDGKHFVRSPMAVKL 250
           F  VGE+K FKV+   K KG   + +VFG L+WSD KH VRSP+ VKL
Sbjct: 727 FTKVGEQKTFKVIL-VKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

[Close]

BLAST of Chy6G124520 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 209.5 bits (532), Expect = 3.1e-54
Identity = 117/278 (42.09%), Postives = 156/278 (56.12%), Query Frame = 0


Query: 11  LGIKPSPMVADFSSRGPNPIIDSMIKPDIAAIRVGCI----------------------- 70
           L  KP+P +A FSSRGPN I   ++KPDI A  V  I                       
Sbjct: 503 LNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNT 562

Query: 71  --------------LTLIQTLYPKWSPAAIKSAIMTTAKTRDNSMKSILDYNKVKATPFQ 130
                         + L++TL+P WSPAAI+SAIMTT++TR+N  K ++D +  KA PF 
Sbjct: 563 ESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFKKANPFS 622

Query: 131 YGAGHVHPNNAIDPGLVYDTTIADYMNFICAQGYNSTTLKKFYNKP-FLCPKSFALTDLN 190
           YG+GHV PN A  PGLVYD T  DY++F+CA GYN+T ++ F   P + C +   L D N
Sbjct: 623 YGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGANLLDFN 682

Query: 191 YPSILVPKLTAGVPVTINRRFKNVGLPGTYVAQVKVSSKVSVTVKPSTLQFNSVGEEKAF 250
           YPSI VP LT    +T+ R+ KNVG P TY A+ +    V V+V+P  L FN  GE K F
Sbjct: 683 YPSITVPNLTGS--ITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEVKIF 742

[Close]

BLAST of Chy6G124520 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 174.5 bits (441), Expect = 1.1e-43
Identity = 106/289 (36.68%), Postives = 153/289 (52.94%), Query Frame = 0


Query: 2   AFITHVKTELGIKPSPMVADFSSRGPNPIIDSMIKPDIAA-------------------- 61
           A I+ + T +G+KPSP+VA FSSRGPN I  +++KPD+ A                    
Sbjct: 469 ASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLAS 528

Query: 62  -----------------IRVGCILTLIQTLYPKWSPAAIKSAIMTTAKTRDNSMKSILDY 121
                              V  +  L+++++P+WSPAAI+SA+MTTA       K +LD 
Sbjct: 529 DSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDI 588

Query: 122 NKVK-ATPFQYGAGHVHPNNAIDPGLVYDTTIADYMNFICAQGYNSTTLKKFYNKPFLC- 181
              K +TPF +GAGHV P  A +PGL+YD T  DY+ F+CA  Y S  ++    + + C 
Sbjct: 589 ATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCD 648

Query: 182 -PKSFALTDLNYPSILVPKLTAGVPVTINRRFKNVGLPGTYVAQVKVSSK---VSVTVKP 241
             KS+++ DLNYPS  V     G      R   +VG  GTY   VKV+S+   V ++V+P
Sbjct: 649 PSKSYSVADLNYPSFAVNVDGVGA-YKYTRTVTSVGGAGTY--SVKVTSETTGVKISVEP 708

Query: 242 STLQFNSVGEEKAFKVVFEYKGKGQGEDHVFGTLIWSDGKHFVRSPMAV 248
           + L F    E+K++ V F          + FG++ WSDGKH V SP+A+
Sbjct: 709 AVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAI 754

[Close]

BLAST of Chy6G124520 vs. TAIR 10
Match: AT4G21640.1 (Subtilase family protein )

HSP 1 Score: 153.3 bits (386), Expect = 2.6e-37
Identity = 100/282 (35.46%), Postives = 144/282 (51.06%), Query Frame = 0


Query: 4   ITHVKTELGIKPSPMVADFSSRGPNPIIDSMIKPDIAAIRVGC----------------- 63
           I+   T  G   +P VA FSSRGPN +  +++KPDIAA  V                   
Sbjct: 452 ISAATTLTGQPATPKVAAFSSRGPNSVSPAILKPDIAAPGVSILAAVSPLDPGAFNGFKL 511

Query: 64  -------------ILTLIQTLYPKWSPAAIKSAIMTTAKTRDNSMKSIL--DYNKVKATP 123
                        I+ L+++L+PKWSPAA++SA++TTA     S + I     NK  A P
Sbjct: 512 HSGTSMSTPVVSGIIVLLKSLHPKWSPAAMRSALVTTAWRTSPSGEPIFAQGSNKKLADP 571

Query: 124 FQYGAGHVHPNNAIDPGLVYDTTIADYMNFICAQGYNSTTLKKFYNKPFLCP-KSFALTD 183
           F YG G V+P  A  PGLVYD  I DY+N++C+ GYN +++ +   K   CP    ++ D
Sbjct: 572 FDYGGGLVNPEKAAKPGLVYDMGIKDYINYMCSAGYNDSSISRVLGKKTKCPIPKPSMLD 631

Query: 184 LNYPSILVPKLTAGVPVTINRRFKNVG-LPGTYVAQVKVSSKVSVTVKPSTLQFNSVGEE 243
           +N PSI +P L     VT+ R   NVG +   Y A ++    +++TV P+ L F S  + 
Sbjct: 632 INLPSITIPNLEK--EVTLTRTVTNVGPIKSVYRAVIESPLGITLTVNPTILVFKSAAKR 691

Query: 244 KAFKVVFEYKGKGQ---GEDHVFGTLIWSDGKHFVRSPMAVK 249
               + F  K K        + FG+L W+DG H V  P++VK
Sbjct: 692 ---VLTFSVKAKTSHKVNSGYFFGSLTWTDGVHDVTIPVSVK 728

[Close]

BLAST of Chy6G124520 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 151.0 bits (380), Expect = 1.3e-36
Identity = 102/287 (35.54%), Postives = 149/287 (51.92%), Query Frame = 0


Query: 9   TELGIKPSPMVADFSSRGPNPIIDSMIKPDIAAIRVGCI--------------------- 68
           T +GIKP+P++A FS RGPN +   ++KPD+ A  V  +                     
Sbjct: 472 TIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEF 531

Query: 69  ----------------LTLIQTLYPKWSPAAIKSAIMTTAKTRDNSMKSILDYNKVK-AT 128
                             L+++ +P WSPA I+SA+MTT    DNS +S++D +  K AT
Sbjct: 532 NILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSAT 591

Query: 129 PFQYGAGHVHPNNAIDPGLVYDTTIADYMNFICAQGYNSTTLKKFYNKPFLCP--KSFAL 188
           P+ YG+GH++   A++PGLVYD T  DY+ F+C+ GY   T++     P  CP  +  + 
Sbjct: 592 PYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSP 651

Query: 189 TDLNYPSI--LVPKLTAG-VPVTINRRFKNVG-LPGTYVAQVKVSSKVSVTVKPSTLQFN 248
            +LNYPSI  + P    G V  T+ R   NVG     Y A+++    V+VTVKP  L F 
Sbjct: 652 GNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFT 711

The following BLAST results are available for this feature:

BLAST of Chy6G124520 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZSP5	2.2e-57	43.75	Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=...	[more]
I1N462	8.7e-54	41.05	Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 P...	[more]
F4JXC5	4.3e-53	42.09	Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S...	[more]
O65351	1.5e-42	36.68	Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S...	[more]
F4JJL8	3.7e-36	35.46	Subtilisin-like protease SBT3.15 OS=Arabidopsis thaliana OX=3702 GN=SBT3.15 PE=2...	[more]

BLAST of Chy6G124520 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3D732	2.2e-121	79.37	Subtilisin-like protease SBT5.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A1S3BLG8	5.0e-121	79.02	subtilisin-like protease SBT5.3 OS=Cucumis melo OX=3656 GN=LOC103491134 PE=3 SV=...	[more]
A0A0A0L601	4.3e-96	63.29	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045060 PE=3 SV=1	[more]
A0A6J1KUL8	2.7e-90	60.84	uncharacterized protein LOC111498820 OS=Cucurbita maxima OX=3661 GN=LOC111498820...	[more]
A0A6J1GDR2	1.2e-87	59.09	subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111453063 PE...	[more]

BLAST of Chy6G124520 vs. NCBI nr


Match Name	E-value	Identity	Description
TYK19386.1	1.73e-142	79.37	subtilisin-like protease SBT5.3 [Cucumis melo var. makuwa]	[more]
XP_008449184.1	7.08e-142	79.02	PREDICTED: subtilisin-like protease SBT5.3 [Cucumis melo]	[more]
XP_038905648.1	4.01e-133	70.73	subtilisin-like protease SBT5.4 [Benincasa hispida]	[more]
XP_038905686.1	7.04e-123	65.73	subtilisin-like protease SBT5.3 [Benincasa hispida]	[more]
KAE8650093.1	7.40e-116	63.64	hypothetical protein Csa_009608 [Cucumis sativus]	[more]

BLAST of Chy6G124520 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G04160.1	1.6e-58	43.75	Subtilisin-like serine endopeptidase family protein	[more]
AT5G59810.1	3.1e-54	42.09	Subtilase family protein	[more]
AT5G67360.1	1.1e-43	36.68	Subtilase family protein	[more]
AT4G21640.1	2.6e-37	35.46	Subtilase family protein	[more]
AT4G34980.1	1.3e-36	35.54	subtilisin-like serine protease 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036852	Peptidase S8/S53 domain superfamily	GENE3D	3.40.50.200	Peptidase S8/S53 domain	coord: 12..41 e-value: 3.1E-6 score: 28.2 coord: 46..118 e-value: 7.4E-23 score: 83.0
IPR036852	Peptidase S8/S53 domain superfamily	SUPERFAMILY	52743	Subtilisin-like	coord: 18..144
IPR041469	Subtilisin-like protease, fibronectin type-III domain	PFAM	PF17766	fn3_6	coord: 150..247 e-value: 2.0E-25 score: 88.8
None	No IPR available	GENE3D	2.60.40.2310		coord: 122..249 e-value: 1.3E-33 score: 117.5
None	No IPR available	PANTHER	PTHR43806	PEPTIDASE S8	coord: 1..45 coord: 44..248
None	No IPR available	PANTHER	PTHR43806:SF34	SUBTILISIN-LIKE PROTEASE SBT5.4	coord: 1..45 coord: 44..248

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy6G124520.1	Chy6G124520.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
biological_process	GO:0009610	response to symbiotic fungus
molecular_function	GO:0004252	serine-type endopeptidase activity