Chy6G116440 (gene) Cucumber (hystrix) v1

Overview
NameChy6G116440
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
LocationchrH06: 9468661 .. 9471930 (-)
RNA-Seq ExpressionChy6G116440
SyntenyChy6G116440
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCGCCGCCATGGCCACTCCTCTCCTCTCCGCAGGTTACTCTGTTACTATCTACGCTCGTATCCTTTCTAGAGCTCTTGATTATCAATCCAAAGGGGCTCTTCTTGTCAACTCTGTTTTTGAATTAGCTCAGACAAGCGATACTGTTTTCACAATGGTGGGCCACCCACAGATGTTCGACAAAATGTCTTAGACGTGACTGGGATCCTGCAAGGCTTGAATCCTGGTGGTGTCATCGTTGACACCACTAGTAGCCACCTCGCTCTTGCCCACGACATATTTACCGCTGCTCTTGCAAAAGGTCGTGTTAAAATTTATTCCACTGAATTACTAGAGTTTGACGACATGACTGAAAGTTTAGGCGGATGAGTTACTCTATGCTATGTTTAATCATTTAGCGATATTCATGGCAGGTTGTTGGGCTGTGGACGCACCGGTGTCAGGGGGTGAGATTGGGGCTAGAGATGGAAAGTTGGCCATTTTTGGTGGTGGAGATGAAGGGGTAGTGAATTGGTTATTACCTTTGTTTGAAGTGTTGGGGAAAGTAACATATGTAGCTGAAGCTGGGTGTGGGCAGCATTGCAAGATAGCAAACCAGATTCCAATTTCGTCGAATTTGTTGGGGCTGTGTGAAGGGTTGGTGTTTGCTGAGCGAGCGGGGCTGGACATGAAGAAGTTTGTGGAAGCTGTCAGGGGAGGGGCAGCATGGTCAATGGCAATGGAGCTGTTTGGTGAGAGGATGATAGGAAGGGACTTTAGGCCTGGTGGGTTTGCTGAGTATATGGTGAATGATTTAGGAATGGGTGGGGTTGTGGAAGAAGGGGAAGATGAGAGAGTTGTAGTGTTGCCAGGAGCTCCATTGACCGATTTAATTCCTTAAGTTTTAAAAATTGGGTTTAAAAGCCCCCTCTACAAAGGAGAATATTAAATATGAGTAAAAATTGGAGTAAATGCTGAAGTAGCACAATGAAACCTTTTTTAAAAATATTATCTCATCTCTCCTGTCAATTAGTGAGTTTTGGAGTGTTAGGTTTTTTGGGTCTTTAAAATGCAATGAAATCAGTTAGAGAGAGAGAGGAAGAAGAGAAAAGGAGAGTAGTGTAGTGTAGTGTAGTGTAGTGTAGTGTAGTGTAGGACCATAGGTGATTTATCTCTTTTCTTTTTCTTTTTCTATACGTAAAAGAGAGAATGAGAGGGAAGAAAATGGGAGAGAGAACGAGGAGAGATGAGATAATATTTAAAAAATGATTTTTCTCTGAGTCATTGTGGCATTCAGTCCAATTTTTGTTTAAATTTAATAATGTTTTTTTTTTGTAAAACCTTTAAAATCTATTCTTTAAAACATGAGGAACTAAAAGCGCCGGTTCCTCAAAACATGGGATTACAAGTGTATTTTTCCCTTCAACATTGATTAAAAGATATAAGGCTTTGAGTGCTAGTCGTAAAGCTTTCGAGGTTTGGTGTCCTGATATTGTTAGTTTTCGGTCAAGTTAAAGTCCTTTGAAATTTTGTCGTTTGCTTTTTTTTCTTCTCTGAATCTCATTTTACCTCTCTGCAGAAAGTCACAACCACAATTCAGAAATCTATCATTTTGCAACTGAGGAGAACAAGAAGATGAATAGGGGGTTTTCTAATAATTCAGATTTAATATTGGATGATGGGATGGAAAAGTTAAAATCTTCCAAAGTAAGAAAGGAGGAGTTTGTTCATTTGGAAAGGATAGATGGCAAACTGAAAAATGTTGTTCAAGAGCTAGAGCTTCTCACAGGAGTTTTTAACGACGAAGAGCAGAAGAACATTGTTGATTATGTTTACCATTTACAACAAAAAGGACGAAGAGCCCAACTTGGAAGTATTTATTCTTCCCATACTTTGCTTTCTAATTCACTTCTTCTCTTGCAATATATGCTCATCATCGTTCTTGTATATTTTCGTATATTTTCAACAGAGCGTACGTATTCCGAAACTAGAAAGTGGATGAGATATATGATGGGAACCGTATGTATTGTAAAGTAAACTTTATTGCTAGTGTGTAGTCAAGGCCTTTACAAGTAGGGAAAGTCTCTTTTCTGTATCTTATCTTTCAAAGATGCACAGAAACCTAACTCTCTATTCACTCAATACAATACTTGCCTTCTCTTTCTTTCTTAGTTTCTCTTTTTATTACCGTAGTATTTAACAGTATTAACTAACTTCTTAACTTCCTGACTAACTTCTTAACAGTCTTCTAACTACTAACTTCTTAACTGTCTTCTAACTAACTTTCTTTCTTCTACTATACTGGTATATGACTGGAGGTCTATCAATATAAGGGAAGAGTTACCATTCAATTTGGATGCTGCTACAATTATGCAATGGTCTTTGACTTCACTGCTTTCTTAACTACCTTCGTATTCATGGTGTTCTTGTTCAGTACAAATTTCTTCAAACCCCTTTGCTTCCTTAGTTGTCTGTCTTATTCTATATTGTCCTTTTGTAGGATAAAAATGGAACTCCACCTTATTAAACAGTTAAAGAAGATTAGAATCTGTTTTTAGTTAGTTAGTTTAACAACTAACAAACTAATTATTTACCTTTCTCACATTTGTACAAATACCCTTGGGTAAGCCATTAATAGAGATGAGAAAGTCACTTATATTTTCCCTTAGACTGTTAATACACCTGGTATCGTTAGAGATGAAGAAGTTGATCCATTGCCATCTTTGTTTAAGAATGAAGGGAGATTGCATTCCTCCACACATTGATCACCATGACTTTCTAAGACCCTTTTGCACTGTGTCATTTCGAACAGAAAGTAACATCATATTTGGCACTCGACTAAAAGTTCTTAATCCTGGAGAGTTCTCTGGTCCTGTCTCGATTCCTTTGCCTAAAGGGTGTCACAAGCACCATTATTGTGCCTTCTTTGTTTGTCCATTTCATTTTCTTGGTTATAAATCTAATCTAATATCTATGCCTCTGTTGATATTGCAGTTCTGAATTGATTTCAACTGGTAATGGAGCTGATCTTGCCAAGCATTGTGTACCTAGTGTCAAAGCTAAAAGGTCGAATATCTACTACTGTTATTTTTACAATCTTCAAATCATTCCCAAAAGAAAAAAGTTCCTAATATTTTGAATCTTTTTGGCATATTGCAGGATATTGATCACACTCCGAAAAATGGATGACAGGAAACTTCCATACAGGTTTTTGCCTGATCTTGAACTTCAAAGAATCAGCCGCTGGACTTATCTAAATGGCAAGAGAAAGAGAACGATAATGAACTAG

mRNA sequence

ATGGGCGCCGCCATGGCCACTCCTCTCCTCTCCGCAGGTTACTCTGTTACTATCTACGCTCACAAGCGATACTGTTTTCACAATGGTGGGCCACCCACAGATGTTCGACAAAATGTCTTAGACGTGACTGGGATCCTGCAAGGCTTGAATCCTGGTGGTGTCATCGTTGACACCACTAGTAGCCACCTCGCTCTTGCCCACGACATATTTACCGCTGCTCTTGCAAAAGGTCGTGTTAAAATTTATTCCACTGAATTACTAGAGTTTGACGACATGACTGAAAGTTGTTGGGCTGTGGACGCACCGGTGTCAGGGGGTGAGATTGGGGCTAGAGATGGAAAGTTGGCCATTTTTGGTGGTGGAGATGAAGGGGTAGTGAATTGGTTATTACCTTTGTTTGAAGTGTTGGGGAAAGTAACATATGTAGCTGAAGCTGGGTGTGGGCAGCATTGCAAGATAGCAAACCAGATTCCAATTTCGTCGAATTTGTTGGGGCTGTGTGAAGGGTTGGTGTTTGCTGAGCGAGCGGGGCTGGACATGAAGAAGTTTGTGGAAGCTGTCAGGGGAGGGGCAGCATGGTCAATGGCAATGGAGCTGTTTGGTGAGAGGATGATAGGAAGGGACTTTAGGCCTGGTGGGTTTGCTGAGTATATGGTGAATGATTTAGGAATGGAAAGTCACAACCACAATTCAGAAATCTATCATTTTGCAACTGAGGAGAACAAGAAGATGAATAGGGGGTTTTCTAATAATTCAGATTTAATATTGGATGATGGGATGGAAAAGTTAAAATCTTCCAAAGTAAGAAAGGAGGAGTTTGTTCATTTGGAAAGGATAGATGGCAAACTGAAAAATGTTGTTCAAGAGCTAGAGCTTCTCACAGGAGTTTTTAACGACGAAGAGCAGAAGAACATTGTTGATTATGTTTACCATTTACAACAAAAAGGACGAAGAGCCCAACTTGGAAAAAGTAACATCATATTTGGCACTCGACTAAAAGTTCTTAATCCTGGAGAGTTCTCTGGTCCTGTCTCGATTCCTTTGCCTAAAGGTTCTGAATTGATTTCAACTGGTAATGGAGCTGATCTTGCCAAGCATTGTGTACCTAGTGTCAAAGCTAAAAGGATATTGATCACACTCCGAAAAATGGATGACAGGAAACTTCCATACAGGTTTTTGCCTGATCTTGAACTTCAAAGAATCAGCCGCTGGACTTATCTAAATGGCAAGAGAAAGAGAACGATAATGAACTAG

Coding sequence (CDS)

ATGGGCGCCGCCATGGCCACTCCTCTCCTCTCCGCAGGTTACTCTGTTACTATCTACGCTCACAAGCGATACTGTTTTCACAATGGTGGGCCACCCACAGATGTTCGACAAAATGTCTTAGACGTGACTGGGATCCTGCAAGGCTTGAATCCTGGTGGTGTCATCGTTGACACCACTAGTAGCCACCTCGCTCTTGCCCACGACATATTTACCGCTGCTCTTGCAAAAGGTCGTGTTAAAATTTATTCCACTGAATTACTAGAGTTTGACGACATGACTGAAAGTTGTTGGGCTGTGGACGCACCGGTGTCAGGGGGTGAGATTGGGGCTAGAGATGGAAAGTTGGCCATTTTTGGTGGTGGAGATGAAGGGGTAGTGAATTGGTTATTACCTTTGTTTGAAGTGTTGGGGAAAGTAACATATGTAGCTGAAGCTGGGTGTGGGCAGCATTGCAAGATAGCAAACCAGATTCCAATTTCGTCGAATTTGTTGGGGCTGTGTGAAGGGTTGGTGTTTGCTGAGCGAGCGGGGCTGGACATGAAGAAGTTTGTGGAAGCTGTCAGGGGAGGGGCAGCATGGTCAATGGCAATGGAGCTGTTTGGTGAGAGGATGATAGGAAGGGACTTTAGGCCTGGTGGGTTTGCTGAGTATATGGTGAATGATTTAGGAATGGAAAGTCACAACCACAATTCAGAAATCTATCATTTTGCAACTGAGGAGAACAAGAAGATGAATAGGGGGTTTTCTAATAATTCAGATTTAATATTGGATGATGGGATGGAAAAGTTAAAATCTTCCAAAGTAAGAAAGGAGGAGTTTGTTCATTTGGAAAGGATAGATGGCAAACTGAAAAATGTTGTTCAAGAGCTAGAGCTTCTCACAGGAGTTTTTAACGACGAAGAGCAGAAGAACATTGTTGATTATGTTTACCATTTACAACAAAAAGGACGAAGAGCCCAACTTGGAAAAAGTAACATCATATTTGGCACTCGACTAAAAGTTCTTAATCCTGGAGAGTTCTCTGGTCCTGTCTCGATTCCTTTGCCTAAAGGTTCTGAATTGATTTCAACTGGTAATGGAGCTGATCTTGCCAAGCATTGTGTACCTAGTGTCAAAGCTAAAAGGATATTGATCACACTCCGAAAAATGGATGACAGGAAACTTCCATACAGGTTTTTGCCTGATCTTGAACTTCAAAGAATCAGCCGCTGGACTTATCTAAATGGCAAGAGAAAGAGAACGATAATGAACTAG

Protein sequence

MGAAMATPLLSAGYSVTIYAHKRYCFHNGGPPTDVRQNVLDVTGILQGLNPGGVIVDTTSSHLALAHDIFTAALAKGRVKIYSTELLEFDDMTESCWAVDAPVSGGEIGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYVAEAGCGQHCKIANQIPISSNLLGLCEGLVFAERAGLDMKKFVEAVRGGAAWSMAMELFGERMIGRDFRPGGFAEYMVNDLGMESHNHNSEIYHFATEENKKMNRGFSNNSDLILDDGMEKLKSSKVRKEEFVHLERIDGKLKNVVQELELLTGVFNDEEQKNIVDYVYHLQQKGRRAQLGKSNIIFGTRLKVLNPGEFSGPVSIPLPKGSELISTGNGADLAKHCVPSVKAKRILITLRKMDDRKLPYRFLPDLELQRISRWTYLNGKRKRTIMN*
Homology
BLAST of Chy6G116440 vs. ExPASy Swiss-Prot
Match: Q949M8 (Probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g71180 PE=2 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 7.2e-53
Identity = 117/249 (46.99%), Postives = 145/249 (58.23%), Query Frame = 0

Query: 1   MGAAMATPLLSAGYSVTIYAH-------------------------KRYCFHNGGPPTDV 60
           MG+AM + +++AGYSVT+YA                              F   G   DV
Sbjct: 45  MGSAMVSHIIAAGYSVTVYARDLRKTKDLQTKGARIANSPKELAEMSDVVFTIVGNFNDV 104

Query: 61  RQNVLDVTGILQGLNPGGVIVDTTSSHLALAHDIFTAALAKGRVKIYSTELLEFDDMTES 120
           R  +L   G+L GL PGGV VD TSS   LA +I   A  +                  +
Sbjct: 105 RSLLLGDDGVLSGLTPGGVTVDMTSSKPGLAREIHAEARRR------------------N 164

Query: 121 CWAVDAPVSGGEIGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYVAEAGCGQHCKIAN 180
           CWAVDAPVSGG+ GAR+G L IF GGD  +V WL P+ + +G VTY+ EAG GQ CKI N
Sbjct: 165 CWAVDAPVSGGDAGAREGTLGIFAGGDSEIVEWLSPVMKNIGTVTYMGEAGSGQSCKIGN 224

Query: 181 QIPISSNLLGLCEGLVFAERAGLDMKKFVEAVRGGAAWSMAMELFGERMIGRDFRPGGFA 225
           QI  +SNL+GL EG+VFAE+AGLD  K++EAV+ GAA S  M LFGE ++ RD+R  GFA
Sbjct: 225 QIAGASNLVGLAEGIVFAEKAGLDTVKWLEAVKDGAAGSAVMRLFGEMIVKRDYRATGFA 275

BLAST of Chy6G116440 vs. ExPASy Swiss-Prot
Match: Q9C991 (Probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g71170 PE=2 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 1.6e-52
Identity = 115/249 (46.18%), Postives = 143/249 (57.43%), Query Frame = 0

Query: 1   MGAAMATPLLSAGYSVTIYAH-------------------------KRYCFHNGGPPTDV 60
           MG+AM + +L+AGYSVT+YA                              F   G   DV
Sbjct: 24  MGSAMVSHILAAGYSVTVYARDLRKTKDLQTKGGRTANSPKELGEMSDVVFTIVGNSNDV 83

Query: 61  RQNVLDVTGILQGLNPGGVIVDTTSSHLALAHDIFTAALAKGRVKIYSTELLEFDDMTES 120
           R  +L   G+L GL PGGV VD TSS   LA +I+  A  +                   
Sbjct: 84  RSLLLGDDGVLSGLKPGGVTVDMTSSKPGLAREIYAEARRR------------------D 143

Query: 121 CWAVDAPVSGGEIGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYVAEAGCGQHCKIAN 180
           CWAVDAPVSGG+ GAR+GKL IF GGD  +V WL P+ + +G V ++  AG GQ CKI N
Sbjct: 144 CWAVDAPVSGGDAGAREGKLTIFAGGDSEIVEWLAPVMKTMGIVRFMGGAGSGQSCKIGN 203

Query: 181 QIPISSNLLGLCEGLVFAERAGLDMKKFVEAVRGGAAWSMAMELFGERMIGRDFRPGGFA 225
           QI + SN++GL EG+VFAE+AGLD  K++EAV+ GAA S  M LFGE M  RD++  GFA
Sbjct: 204 QICVGSNMIGLAEGIVFAEKAGLDPVKWLEAVKDGAAGSAVMRLFGEMMAVRDYKATGFA 254

BLAST of Chy6G116440 vs. ExPASy Swiss-Prot
Match: Q9SZE1 (Probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g29120 PE=1 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 4.2e-45
Identity = 103/250 (41.20%), Postives = 148/250 (59.20%), Query Frame = 0

Query: 1   MGAAMATPLLSAGYSVTIY-------------------------AHKRYCFHNGGPPTDV 60
           MG +M   L+ AGY+VT++                               F   G P+DV
Sbjct: 48  MGRSMCGHLIKAGYTVTVFNRTISKAQTLIDMGANVADSPNSVAEQSDVVFTIVGYPSDV 107

Query: 61  RQNVLD-VTGILQGLNPGGVIVDTTSSHLALAHDIFTAALAKGRVKIYSTELLEFDDMTE 120
           R  +LD  +G L GL  GGV+VD T+S  +LA +I  AA  K                  
Sbjct: 108 RHVLLDPKSGALSGLRQGGVLVDMTTSEPSLAEEIAKAASFK------------------ 167

Query: 121 SCWAVDAPVSGGEIGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYVAEAGCGQHCKIA 180
           +C+++DAPVSGG++GA++GKL+IF GGDE  V  L PLF ++GKV ++  +G GQ  K+A
Sbjct: 168 NCFSIDAPVSGGDLGAKNGKLSIFAGGDETTVKRLDPLFSLMGKVNFMGTSGKGQFAKLA 227

Query: 181 NQIPISSNLLGLCEGLVFAERAGLDMKKFVEAVRGGAAWSMAMELFGERMIGRDFRPGGF 225
           NQI I+S +LGL EGL++A +AGLD+KKF+EA+  GAA S +++L+G+R++ RDF PG +
Sbjct: 228 NQITIASTMLGLVEGLIYAHKAGLDVKKFLEAISTGAAGSKSIDLYGDRILKRDFDPGFY 279

BLAST of Chy6G116440 vs. ExPASy Swiss-Prot
Match: O34948 (Uncharacterized oxidoreductase YkwC OS=Bacillus subtilis (strain 168) OX=224308 GN=ykwC PE=3 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 3.0e-27
Identity = 72/250 (28.80%), Postives = 128/250 (51.20%), Query Frame = 0

Query: 1   MGAAMATPLLSAGYSVTIYAHKR----YCFHNG---------------------GPPTDV 60
           MG +MA+ +L+ G+ V +Y   +         G                     G P+DV
Sbjct: 13  MGKSMASHILNDGHPVLVYTRTKEKAESILQKGAIWKDTVKDLSKEADVIITMVGYPSDV 72

Query: 61  RQNVLDVTGILQGLNPGGVIVDTTSSHLALAHDIFTAALAKGRVKIYSTELLEFDDMTES 120
            +      GI++    G  ++D T+S  +LA  I  AA  K                  +
Sbjct: 73  EEVYFGSNGIIENAKEGAYLIDMTTSKPSLAKKIAEAAKEK------------------A 132

Query: 121 CWAVDAPVSGGEIGARDGKLAIFGGGDEGVVNWLLPLFEVLGK-VTYVAEAGCGQHCKIA 180
            +A+DAPVSGG+IGA++G LAI  GG++      +P+F ++G+ + Y   AG GQH K+ 
Sbjct: 133 LFALDAPVSGGDIGAQNGTLAIMVGGEKEAFEACMPIFSLMGENIQYQGPAGSGQHTKMC 192

Query: 181 NQIPISSNLLGLCEGLVFAERAGLDMKKFVEAVRGGAAWSMAMELFGERMIGRDFRPGGF 225
           NQI I++ ++G+ E + +A+++GL+ +  ++++  GAA S ++     RM+  +F PG +
Sbjct: 193 NQIAIAAGMIGVAEAMAYAQKSGLEPENVLKSITTGAAGSWSLSNLAPRMLQGNFEPGFY 244

BLAST of Chy6G116440 vs. ExPASy Swiss-Prot
Match: P77161 (2-hydroxy-3-oxopropionate reductase OS=Escherichia coli (strain K12) OX=83333 GN=glxR PE=2 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 6.3e-25
Identity = 61/115 (53.04%), Postives = 79/115 (68.70%), Query Frame = 0

Query: 99  VDAPVSGGEIGARDGKLAIFGGGDEGVVNWLLPLFEVLGK-VTYVAEAGCGQHCKIANQI 158
           +DAPVSGGEIGAR+G L+I  GGDE V   + PLFE+LGK +T V   G GQ CK+ANQI
Sbjct: 115 LDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVGGNGDGQTCKVANQI 174

Query: 159 PISSNLLGLCEGLVFAERAGLDMKKFVEAVRGGAAWSMAMELFGERMIGRDFRPG 213
            ++ N+  + E L+FA +AG D  +  +A+ GG A S  +E+ GERMI R F PG
Sbjct: 175 IVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHGERMIKRTFNPG 229

BLAST of Chy6G116440 vs. ExPASy TrEMBL
Match: A0A5A7TCQ0 (Putative 3-hydroxyisobutyrate dehydrogenase-like 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold84G001550 PE=4 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 5.1e-86
Identity = 177/249 (71.08%), Postives = 185/249 (74.30%), Query Frame = 0

Query: 1   MGAAMATPLLSAGYSVTIYAH-------------------------KRYCFHNGGPPTDV 60
           MGAAMAT LLSAGYSVTIYA                              F   G P+DV
Sbjct: 24  MGAAMATRLLSAGYSVTIYARSPSKALDLQSKGALLVNSVLELAQTSDIVFTMVGHPSDV 83

Query: 61  RQNVLDVTGILQGLNPGGVIVDTTSSHLALAHDIFTAALAKGRVKIYSTELLEFDDMTES 120
           RQNVLD  GILQGLNPGGVIVDTTSSH ALA +IFTAA AKG                  
Sbjct: 84  RQNVLDDNGILQGLNPGGVIVDTTSSHPALARNIFTAARAKG------------------ 143

Query: 121 CWAVDAPVSGGEIGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYVAEAGCGQHCKIAN 180
           CWAVDAPVSGG+IGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYV EAGCGQ CKIAN
Sbjct: 144 CWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYVGEAGCGQSCKIAN 203

Query: 181 QIPISSNLLGLCEGLVFAERAGLDMKKFVEAVRGGAAWSMAMELFGERMIGRDFRPGGFA 225
           QIPIS+NL+GL EGL+FAERAGLD KKF+EAVRGGAA SMAMELFGERMIGRDFRPGG+A
Sbjct: 204 QIPISANLMGLSEGLMFAERAGLDTKKFLEAVRGGAAGSMAMELFGERMIGRDFRPGGYA 254

BLAST of Chy6G116440 vs. ExPASy TrEMBL
Match: A0A1S3C3N4 (probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103496122 PE=4 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 5.1e-86
Identity = 177/249 (71.08%), Postives = 185/249 (74.30%), Query Frame = 0

Query: 1   MGAAMATPLLSAGYSVTIYAH-------------------------KRYCFHNGGPPTDV 60
           MGAAMAT LLSAGYSVTIYA                              F   G P+DV
Sbjct: 24  MGAAMATRLLSAGYSVTIYARSPSKALDLQSKGALLVNSVLELAQTSDIVFTMVGHPSDV 83

Query: 61  RQNVLDVTGILQGLNPGGVIVDTTSSHLALAHDIFTAALAKGRVKIYSTELLEFDDMTES 120
           RQNVLD  GILQGLNPGGVIVDTTSSH ALA +IFTAA AKG                  
Sbjct: 84  RQNVLDDNGILQGLNPGGVIVDTTSSHPALARNIFTAARAKG------------------ 143

Query: 121 CWAVDAPVSGGEIGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYVAEAGCGQHCKIAN 180
           CWAVDAPVSGG+IGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYV EAGCGQ CKIAN
Sbjct: 144 CWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYVGEAGCGQSCKIAN 203

Query: 181 QIPISSNLLGLCEGLVFAERAGLDMKKFVEAVRGGAAWSMAMELFGERMIGRDFRPGGFA 225
           QIPIS+NL+GL EGL+FAERAGLD KKF+EAVRGGAA SMAMELFGERMIGRDFRPGG+A
Sbjct: 204 QIPISANLMGLSEGLMFAERAGLDTKKFLEAVRGGAAGSMAMELFGERMIGRDFRPGGYA 254

BLAST of Chy6G116440 vs. ExPASy TrEMBL
Match: A0A0A0LC06 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G214010 PE=4 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 6.7e-86
Identity = 175/249 (70.28%), Postives = 183/249 (73.49%), Query Frame = 0

Query: 1   MGAAMATPLLSAGYSVTIYAH-------------------------KRYCFHNGGPPTDV 60
           MGAAMAT LLSAGYS++IYA                              F   G P+DV
Sbjct: 24  MGAAMATRLLSAGYSLSIYARTPSKALDLQSKGALLVNSVLELAQVSDIVFTMVGHPSDV 83

Query: 61  RQNVLDVTGILQGLNPGGVIVDTTSSHLALAHDIFTAALAKGRVKIYSTELLEFDDMTES 120
           RQNVLD  GILQGLNPGGV+VDTTSSH ALA DIFTAA AKG                  
Sbjct: 84  RQNVLDENGILQGLNPGGVVVDTTSSHPALARDIFTAARAKG------------------ 143

Query: 121 CWAVDAPVSGGEIGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYVAEAGCGQHCKIAN 180
           CWAVDAPVSGG+IGARDGKLAIFGGGDEGVVNWLLPLF+VLGKVTYV EAGCGQ CKIAN
Sbjct: 144 CWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFQVLGKVTYVGEAGCGQSCKIAN 203

Query: 181 QIPISSNLLGLCEGLVFAERAGLDMKKFVEAVRGGAAWSMAMELFGERMIGRDFRPGGFA 225
           Q  IS+NLLGL EGLVFAERAGLDMKKFVEAVRGG AWSMAMELF ERMIGRDFRPGGFA
Sbjct: 204 QFAISANLLGLSEGLVFAERAGLDMKKFVEAVRGGGAWSMAMELFAERMIGRDFRPGGFA 254

BLAST of Chy6G116440 vs. ExPASy TrEMBL
Match: A0A0A0L752 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G217150 PE=4 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 8.7e-86
Identity = 176/249 (70.68%), Postives = 182/249 (73.09%), Query Frame = 0

Query: 1   MGAAMATPLLSAGYSVTIYAH-------------------------KRYCFHNGGPPTDV 60
           MGAAMAT LLSAGYS++IYA                              F   G P+DV
Sbjct: 24  MGAAMATRLLSAGYSLSIYARTPSKALDLQSKGALLVNSVLELAQVSDIVFTMVGHPSDV 83

Query: 61  RQNVLDVTGILQGLNPGGVIVDTTSSHLALAHDIFTAALAKGRVKIYSTELLEFDDMTES 120
           RQNVLD  GILQGLNPGGVIVDTTSSH ALA DIF AA AKG                  
Sbjct: 84  RQNVLDENGILQGLNPGGVIVDTTSSHPALARDIFIAARAKG------------------ 143

Query: 121 CWAVDAPVSGGEIGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYVAEAGCGQHCKIAN 180
           CWAVDAPVSGG+IGARDGKLAIFGGGDEGVVNWLLPLF+VLGKVTYV EAGCGQ CKIAN
Sbjct: 144 CWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFQVLGKVTYVGEAGCGQSCKIAN 203

Query: 181 QIPISSNLLGLCEGLVFAERAGLDMKKFVEAVRGGAAWSMAMELFGERMIGRDFRPGGFA 225
           Q  IS+NLLGL EGLVFAERAGLDMKKFVEAVRGG AWSMAMELF ERMIGRDFRPGGFA
Sbjct: 204 QFAISANLLGLSEGLVFAERAGLDMKKFVEAVRGGGAWSMAMELFAERMIGRDFRPGGFA 254

BLAST of Chy6G116440 vs. ExPASy TrEMBL
Match: A0A5D3BNN2 (Putative 3-hydroxyisobutyrate dehydrogenase-like 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold252G00250 PE=4 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 1.9e-85
Identity = 176/249 (70.68%), Postives = 184/249 (73.90%), Query Frame = 0

Query: 1   MGAAMATPLLSAGYSVTIYAH-------------------------KRYCFHNGGPPTDV 60
           MGAAMAT LL AGYSVTIYA                              F   G P+DV
Sbjct: 24  MGAAMATRLLCAGYSVTIYARSPSKALDLQSKGALLVNSVLELAQTSDIVFTMVGHPSDV 83

Query: 61  RQNVLDVTGILQGLNPGGVIVDTTSSHLALAHDIFTAALAKGRVKIYSTELLEFDDMTES 120
           RQNVLD  GILQGLNPGGVIVDTTSSH ALA +IFTAA AKG                  
Sbjct: 84  RQNVLDDNGILQGLNPGGVIVDTTSSHPALARNIFTAARAKG------------------ 143

Query: 121 CWAVDAPVSGGEIGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYVAEAGCGQHCKIAN 180
           CWAVDAPVSGG+IGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYV EAGCGQ CKIAN
Sbjct: 144 CWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYVGEAGCGQSCKIAN 203

Query: 181 QIPISSNLLGLCEGLVFAERAGLDMKKFVEAVRGGAAWSMAMELFGERMIGRDFRPGGFA 225
           QIPIS+NL+GL EGL+FAERAGLD KKF+EAVRGGAA SMAMELFGERMIGRDFRPGG+A
Sbjct: 204 QIPISANLMGLSEGLMFAERAGLDTKKFLEAVRGGAAGSMAMELFGERMIGRDFRPGGYA 254

BLAST of Chy6G116440 vs. NCBI nr
Match: KAE8650518.1 (hypothetical protein Csa_009596 [Cucumis sativus])

HSP 1 Score: 513 bits (1322), Expect = 1.07e-178
Identity = 289/442 (65.38%), Postives = 302/442 (68.33%), Query Frame = 0

Query: 1   MGAAMATPLLSAGYSVTIYAH-------------------------KRYCFHNGGPPTDV 60
           MGAAMAT LLSAGYS+++YA                              F   G P DV
Sbjct: 24  MGAAMATRLLSAGYSLSLYARTPSKALDLQSKGALLVNSVLELAQVSDIVFTMVGHPLDV 83

Query: 61  RQNVLDVTGILQGLNPGGVIVDTTSSHLALAHDIFTAALAKGRVKIYSTELLEFDDMTES 120
           RQNVLD  GILQGLNPGGV+VDTTSSH ALA DIFTAA AKG                  
Sbjct: 84  RQNVLDENGILQGLNPGGVVVDTTSSHPALARDIFTAARAKG------------------ 143

Query: 121 CWAVDAPVSGGEIGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYVAEAGCGQHCKIAN 180
           CWAVDAPVSGG+IGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYV EAGCGQ CKIAN
Sbjct: 144 CWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYVGEAGCGQSCKIAN 203

Query: 181 QIPISSNLLGLCEGLVFAERAGLDMKKFVEAVRGGAAWSMAMELFGERMIGRDFRPGGFA 240
           Q PIS+NLLGL EGLVFAERAGLDMKKFVEAVRGG AWSMAMELFGERMIGRDFRPGGFA
Sbjct: 204 QFPISANLLGLSEGLVFAERAGLDMKKFVEAVRGGGAWSMAMELFGERMIGRDFRPGGFA 263

Query: 241 EYMVNDLGMESHNHNSEIYHFATEENKKMNRGFSNNSDLILDDGMEKLKSSKVRKEEFVH 300
           EYMV DLG+ESHNHNSEIYHFATEEN+K NRGFSNNSDLILDDGMEKLKSSK        
Sbjct: 264 EYMVKDLGIESHNHNSEIYHFATEENRKRNRGFSNNSDLILDDGMEKLKSSKTVN----- 323

Query: 301 LERIDGKLKNVVQELELLTGVFNDEEQKNIVDYVYHLQQKGRRAQLGKSNIIFGTRLKVL 360
                              G+  DEE    VD +  L +K  R                 
Sbjct: 324 -----------------TPGILRDEE----VDPLPSLFKKMIR----------------- 383

Query: 361 NPGEFSGPVSIPLPKGSELISTGNGADLAKHCVPSVKAKRILITLRKMDDRKLPYRFLPD 417
                         + S LIS+GNG DLAKHCVPSVKAKRILITLRK DDRKLPYRFLPD
Sbjct: 384 --------------ECSVLISSGNGPDLAKHCVPSVKAKRILITLRKKDDRKLPYRFLPD 390

BLAST of Chy6G116440 vs. NCBI nr
Match: XP_031738997.1 (probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial [Cucumis sativus] >XP_031738998.1 probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial [Cucumis sativus] >XP_031738999.1 probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial [Cucumis sativus] >XP_031739000.1 probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial [Cucumis sativus] >XP_031739001.1 probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial [Cucumis sativus] >XP_031739002.1 probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial [Cucumis sativus])

HSP 1 Score: 333 bits (854), Expect = 2.39e-109
Identity = 177/249 (71.08%), Postives = 184/249 (73.90%), Query Frame = 0

Query: 1   MGAAMATPLLSAGYSVTIYAH-------------------------KRYCFHNGGPPTDV 60
           MGAAMAT LLSAGYS+++YA                              F   G P DV
Sbjct: 24  MGAAMATRLLSAGYSLSLYARTPSKALDLQSKGALLVNSVLELAQVSDIVFTMVGHPLDV 83

Query: 61  RQNVLDVTGILQGLNPGGVIVDTTSSHLALAHDIFTAALAKGRVKIYSTELLEFDDMTES 120
           RQNVLD  GILQGLNPGGV+VDTTSSH ALA DIFTAA AKG                  
Sbjct: 84  RQNVLDENGILQGLNPGGVVVDTTSSHPALARDIFTAARAKG------------------ 143

Query: 121 CWAVDAPVSGGEIGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYVAEAGCGQHCKIAN 180
           CWAVDAPVSGG+IGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYV EAGCGQ CKIAN
Sbjct: 144 CWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYVGEAGCGQSCKIAN 203

Query: 181 QIPISSNLLGLCEGLVFAERAGLDMKKFVEAVRGGAAWSMAMELFGERMIGRDFRPGGFA 224
           Q PIS+NLLGL EGLVFAERAGLDMKKFVEAVRGG AWSMAMELFGERMIGRDFRPGGFA
Sbjct: 204 QFPISANLLGLSEGLVFAERAGLDMKKFVEAVRGGGAWSMAMELFGERMIGRDFRPGGFA 254

BLAST of Chy6G116440 vs. NCBI nr
Match: XP_008456080.1 (PREDICTED: probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial [Cucumis melo])

HSP 1 Score: 328 bits (842), Expect = 3.53e-107
Identity = 177/249 (71.08%), Postives = 185/249 (74.30%), Query Frame = 0

Query: 1   MGAAMATPLLSAGYSVTIYAHKR-------------------------YCFHNGGPPTDV 60
           MGAAMAT LLSAGYSVTIYA                              F   G P+DV
Sbjct: 24  MGAAMATRLLSAGYSVTIYARSPSKALDLQSKGALLVNSVLELAQTSDIVFTMVGHPSDV 83

Query: 61  RQNVLDVTGILQGLNPGGVIVDTTSSHLALAHDIFTAALAKGRVKIYSTELLEFDDMTES 120
           RQNVLD  GILQGLNPGGVIVDTTSSH ALA +IFTAA AKG                  
Sbjct: 84  RQNVLDDNGILQGLNPGGVIVDTTSSHPALARNIFTAARAKG------------------ 143

Query: 121 CWAVDAPVSGGEIGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYVAEAGCGQHCKIAN 180
           CWAVDAPVSGG+IGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYV EAGCGQ CKIAN
Sbjct: 144 CWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYVGEAGCGQSCKIAN 203

Query: 181 QIPISSNLLGLCEGLVFAERAGLDMKKFVEAVRGGAAWSMAMELFGERMIGRDFRPGGFA 224
           QIPIS+NL+GL EGL+FAERAGLD KKF+EAVRGGAA SMAMELFGERMIGRDFRPGG+A
Sbjct: 204 QIPISANLMGLSEGLMFAERAGLDTKKFLEAVRGGAAGSMAMELFGERMIGRDFRPGGYA 254

BLAST of Chy6G116440 vs. NCBI nr
Match: XP_004149969.3 (probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial [Cucumis sativus] >KAE8650526.1 hypothetical protein Csa_011735 [Cucumis sativus])

HSP 1 Score: 328 bits (840), Expect = 7.09e-107
Identity = 176/249 (70.68%), Postives = 182/249 (73.09%), Query Frame = 0

Query: 1   MGAAMATPLLSAGYSVTIYAH-------------------------KRYCFHNGGPPTDV 60
           MGAAMAT LLSAGYS++IYA                              F   G P+DV
Sbjct: 24  MGAAMATRLLSAGYSLSIYARTPSKALDLQSKGALLVNSVLQLAQTSDIVFTMVGHPSDV 83

Query: 61  RQNVLDVTGILQGLNPGGVIVDTTSSHLALAHDIFTAALAKGRVKIYSTELLEFDDMTES 120
           RQNVLD  GILQGLNPGGVIVDTTSSH ALA DIF AA AKG                  
Sbjct: 84  RQNVLDENGILQGLNPGGVIVDTTSSHPALARDIFIAARAKG------------------ 143

Query: 121 CWAVDAPVSGGEIGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYVAEAGCGQHCKIAN 180
           CWAVDAPVSGG+IGARDGKLAIFGGGDEGVVNWLLPLF+VLGKVTYV EAGCGQ CKIAN
Sbjct: 144 CWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFQVLGKVTYVGEAGCGQSCKIAN 203

Query: 181 QIPISSNLLGLCEGLVFAERAGLDMKKFVEAVRGGAAWSMAMELFGERMIGRDFRPGGFA 224
           Q  IS+NLLGL EGLVFAERAGLDMKKFVEAVRGG AWSMAMELF ERMIGRDFRPGGFA
Sbjct: 204 QFAISANLLGLSEGLVFAERAGLDMKKFVEAVRGGGAWSMAMELFAERMIGRDFRPGGFA 254

BLAST of Chy6G116440 vs. NCBI nr
Match: KAA0039059.1 (putative 3-hydroxyisobutyrate dehydrogenase-like 3 [Cucumis melo var. makuwa])

HSP 1 Score: 328 bits (842), Expect = 3.94e-105
Identity = 177/249 (71.08%), Postives = 185/249 (74.30%), Query Frame = 0

Query: 1   MGAAMATPLLSAGYSVTIYAHKR-------------------------YCFHNGGPPTDV 60
           MGAAMAT LLSAGYSVTIYA                              F   G P+DV
Sbjct: 24  MGAAMATRLLSAGYSVTIYARSPSKALDLQSKGALLVNSVLELAQTSDIVFTMVGHPSDV 83

Query: 61  RQNVLDVTGILQGLNPGGVIVDTTSSHLALAHDIFTAALAKGRVKIYSTELLEFDDMTES 120
           RQNVLD  GILQGLNPGGVIVDTTSSH ALA +IFTAA AKG                  
Sbjct: 84  RQNVLDDNGILQGLNPGGVIVDTTSSHPALARNIFTAARAKG------------------ 143

Query: 121 CWAVDAPVSGGEIGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYVAEAGCGQHCKIAN 180
           CWAVDAPVSGG+IGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYV EAGCGQ CKIAN
Sbjct: 144 CWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYVGEAGCGQSCKIAN 203

Query: 181 QIPISSNLLGLCEGLVFAERAGLDMKKFVEAVRGGAAWSMAMELFGERMIGRDFRPGGFA 224
           QIPIS+NL+GL EGL+FAERAGLD KKF+EAVRGGAA SMAMELFGERMIGRDFRPGG+A
Sbjct: 204 QIPISANLMGLSEGLMFAERAGLDTKKFLEAVRGGAAGSMAMELFGERMIGRDFRPGGYA 254

BLAST of Chy6G116440 vs. TAIR 10
Match: AT1G71180.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 209.5 bits (532), Expect = 5.1e-54
Identity = 117/249 (46.99%), Postives = 145/249 (58.23%), Query Frame = 0

Query: 1   MGAAMATPLLSAGYSVTIYAH-------------------------KRYCFHNGGPPTDV 60
           MG+AM + +++AGYSVT+YA                              F   G   DV
Sbjct: 45  MGSAMVSHIIAAGYSVTVYARDLRKTKDLQTKGARIANSPKELAEMSDVVFTIVGNFNDV 104

Query: 61  RQNVLDVTGILQGLNPGGVIVDTTSSHLALAHDIFTAALAKGRVKIYSTELLEFDDMTES 120
           R  +L   G+L GL PGGV VD TSS   LA +I   A  +                  +
Sbjct: 105 RSLLLGDDGVLSGLTPGGVTVDMTSSKPGLAREIHAEARRR------------------N 164

Query: 121 CWAVDAPVSGGEIGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYVAEAGCGQHCKIAN 180
           CWAVDAPVSGG+ GAR+G L IF GGD  +V WL P+ + +G VTY+ EAG GQ CKI N
Sbjct: 165 CWAVDAPVSGGDAGAREGTLGIFAGGDSEIVEWLSPVMKNIGTVTYMGEAGSGQSCKIGN 224

Query: 181 QIPISSNLLGLCEGLVFAERAGLDMKKFVEAVRGGAAWSMAMELFGERMIGRDFRPGGFA 225
           QI  +SNL+GL EG+VFAE+AGLD  K++EAV+ GAA S  M LFGE ++ RD+R  GFA
Sbjct: 225 QIAGASNLVGLAEGIVFAEKAGLDTVKWLEAVKDGAAGSAVMRLFGEMIVKRDYRATGFA 275

BLAST of Chy6G116440 vs. TAIR 10
Match: AT1G71170.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 208.4 bits (529), Expect = 1.1e-53
Identity = 115/249 (46.18%), Postives = 143/249 (57.43%), Query Frame = 0

Query: 1   MGAAMATPLLSAGYSVTIYAH-------------------------KRYCFHNGGPPTDV 60
           MG+AM + +L+AGYSVT+YA                              F   G   DV
Sbjct: 24  MGSAMVSHILAAGYSVTVYARDLRKTKDLQTKGGRTANSPKELGEMSDVVFTIVGNSNDV 83

Query: 61  RQNVLDVTGILQGLNPGGVIVDTTSSHLALAHDIFTAALAKGRVKIYSTELLEFDDMTES 120
           R  +L   G+L GL PGGV VD TSS   LA +I+  A  +                   
Sbjct: 84  RSLLLGDDGVLSGLKPGGVTVDMTSSKPGLAREIYAEARRR------------------D 143

Query: 121 CWAVDAPVSGGEIGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYVAEAGCGQHCKIAN 180
           CWAVDAPVSGG+ GAR+GKL IF GGD  +V WL P+ + +G V ++  AG GQ CKI N
Sbjct: 144 CWAVDAPVSGGDAGAREGKLTIFAGGDSEIVEWLAPVMKTMGIVRFMGGAGSGQSCKIGN 203

Query: 181 QIPISSNLLGLCEGLVFAERAGLDMKKFVEAVRGGAAWSMAMELFGERMIGRDFRPGGFA 225
           QI + SN++GL EG+VFAE+AGLD  K++EAV+ GAA S  M LFGE M  RD++  GFA
Sbjct: 204 QICVGSNMIGLAEGIVFAEKAGLDPVKWLEAVKDGAAGSAVMRLFGEMMAVRDYKATGFA 254

BLAST of Chy6G116440 vs. TAIR 10
Match: AT4G29120.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 183.7 bits (465), Expect = 3.0e-46
Identity = 103/250 (41.20%), Postives = 148/250 (59.20%), Query Frame = 0

Query: 1   MGAAMATPLLSAGYSVTIY-------------------------AHKRYCFHNGGPPTDV 60
           MG +M   L+ AGY+VT++                               F   G P+DV
Sbjct: 48  MGRSMCGHLIKAGYTVTVFNRTISKAQTLIDMGANVADSPNSVAEQSDVVFTIVGYPSDV 107

Query: 61  RQNVLD-VTGILQGLNPGGVIVDTTSSHLALAHDIFTAALAKGRVKIYSTELLEFDDMTE 120
           R  +LD  +G L GL  GGV+VD T+S  +LA +I  AA  K                  
Sbjct: 108 RHVLLDPKSGALSGLRQGGVLVDMTTSEPSLAEEIAKAASFK------------------ 167

Query: 121 SCWAVDAPVSGGEIGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYVAEAGCGQHCKIA 180
           +C+++DAPVSGG++GA++GKL+IF GGDE  V  L PLF ++GKV ++  +G GQ  K+A
Sbjct: 168 NCFSIDAPVSGGDLGAKNGKLSIFAGGDETTVKRLDPLFSLMGKVNFMGTSGKGQFAKLA 227

Query: 181 NQIPISSNLLGLCEGLVFAERAGLDMKKFVEAVRGGAAWSMAMELFGERMIGRDFRPGGF 225
           NQI I+S +LGL EGL++A +AGLD+KKF+EA+  GAA S +++L+G+R++ RDF PG +
Sbjct: 228 NQITIASTMLGLVEGLIYAHKAGLDVKKFLEAISTGAAGSKSIDLYGDRILKRDFDPGFY 279

BLAST of Chy6G116440 vs. TAIR 10
Match: AT2G17970.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 113.2 bits (282), Expect = 5.0e-25
Identity = 89/274 (32.48%), Postives = 112/274 (40.88%), Query Frame = 0

Query: 239 EENKKMNRGFSNNS------DLILDDGMEKLKSSKV-RKEEFVHLERIDGKLKNVVQELE 298
           EE     +GF  +S        +  D  E L+   V RK++F+ LER+ GK+ NV+  LE
Sbjct: 158 EERDSSRKGFDASSMKTPEKPKLSRDQRENLRLINVKRKKDFICLERVKGKIVNVLDGLE 217

Query: 299 LLTGVFNDEEQKNIVDYVYHLQQKGRRAQLGK---------------------------- 358
           L TGVF+  EQK IVD VY LQ+KGRR +L K                            
Sbjct: 218 LHTGVFSAVEQKRIVDQVYQLQEKGRRGELKKRTFTAPHKWMRGKGRETIQFGCCYNYAP 277

Query: 359 ------------------------------------------------------------ 402
                                                                       
Sbjct: 278 DRAGNPPGILQREEVDPLPHLFKVIIRKLIKWHVLPPTCVPDSCIVNIYDEGDCIPPHID 337

BLAST of Chy6G116440 vs. TAIR 10
Match: AT2G17970.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 113.2 bits (282), Expect = 5.0e-25
Identity = 89/274 (32.48%), Postives = 112/274 (40.88%), Query Frame = 0

Query: 239 EENKKMNRGFSNNS------DLILDDGMEKLKSSKV-RKEEFVHLERIDGKLKNVVQELE 298
           EE     +GF  +S        +  D  E L+   V RK++F+ LER+ GK+ NV+  LE
Sbjct: 84  EERDSSRKGFDASSMKTPEKPKLSRDQRENLRLINVKRKKDFICLERVKGKIVNVLDGLE 143

Query: 299 LLTGVFNDEEQKNIVDYVYHLQQKGRRAQLGK---------------------------- 358
           L TGVF+  EQK IVD VY LQ+KGRR +L K                            
Sbjct: 144 LHTGVFSAVEQKRIVDQVYQLQEKGRRGELKKRTFTAPHKWMRGKGRETIQFGCCYNYAP 203

Query: 359 ------------------------------------------------------------ 402
                                                                       
Sbjct: 204 DRAGNPPGILQREEVDPLPHLFKVIIRKLIKWHVLPPTCVPDSCIVNIYDEGDCIPPHID 263

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q949M87.2e-5346.99Probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial OS=Arabidopsis... [more]
Q9C9911.6e-5246.18Probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial OS=Arabidopsis... [more]
Q9SZE14.2e-4541.20Probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Arabidopsis... [more]
O349483.0e-2728.80Uncharacterized oxidoreductase YkwC OS=Bacillus subtilis (strain 168) OX=224308 ... [more]
P771616.3e-2553.042-hydroxy-3-oxopropionate reductase OS=Escherichia coli (strain K12) OX=83333 GN... [more]
Match NameE-valueIdentityDescription
A0A5A7TCQ05.1e-8671.08Putative 3-hydroxyisobutyrate dehydrogenase-like 3 OS=Cucumis melo var. makuwa O... [more]
A0A1S3C3N45.1e-8671.08probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial OS=Cucumis mel... [more]
A0A0A0LC066.7e-8670.28Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G214010 PE=4 SV=1[more]
A0A0A0L7528.7e-8670.68Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G217150 PE=4 SV=1[more]
A0A5D3BNN21.9e-8570.68Putative 3-hydroxyisobutyrate dehydrogenase-like 3 OS=Cucumis melo var. makuwa O... [more]
Match NameE-valueIdentityDescription
KAE8650518.11.07e-17865.38hypothetical protein Csa_009596 [Cucumis sativus][more]
XP_031738997.12.39e-10971.08probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial [Cucumis sativ... [more]
XP_008456080.13.53e-10771.08PREDICTED: probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial [Cu... [more]
XP_004149969.37.09e-10770.68probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial [Cucumis sativ... [more]
KAA0039059.13.94e-10571.08putative 3-hydroxyisobutyrate dehydrogenase-like 3 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT1G71180.15.1e-5446.996-phosphogluconate dehydrogenase family protein [more]
AT1G71170.11.1e-5346.186-phosphogluconate dehydrogenase family protein [more]
AT4G29120.13.0e-4641.206-phosphogluconate dehydrogenase family protein [more]
AT2G17970.15.0e-2532.482-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT2G17970.25.0e-2532.482-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0061156-phosphogluconate dehydrogenase, NADP-bindingPFAMPF03446NAD_binding_2coord: 41..140
e-value: 3.8E-10
score: 40.2
IPR037151Alpha-ketoglutarate-dependent dioxygenase AlkB-like superfamilyGENE3D2.60.120.590coord: 324..388
e-value: 2.6E-12
score: 48.7
coord: 272..323
e-value: 2.7E-5
score: 25.8
IPR0133286-phosphogluconate dehydrogenase, domain 2GENE3D1.10.1040.10coord: 144..227
e-value: 2.2E-21
score: 78.5
IPR0291543-hydroxyisobutyrate dehydrogenase, NAD-binding domainPFAMPF14833NAD_binding_11coord: 146..224
e-value: 4.5E-19
score: 68.9
NoneNo IPR availableGENE3D3.40.50.720coord: 30..143
e-value: 2.1E-14
score: 55.3
NoneNo IPR availablePANTHERPTHR43060:SF133-HYDROXYISOBUTYRATE DEHYDROGENASE-LIKE 2, MITOCHONDRIAL-RELATEDcoord: 94..228
NoneNo IPR availablePANTHERPTHR43060:SF133-HYDROXYISOBUTYRATE DEHYDROGENASE-LIKE 2, MITOCHONDRIAL-RELATEDcoord: 30..77
NoneNo IPR availablePANTHERPTHR430603-HYDROXYISOBUTYRATE DEHYDROGENASE-LIKE 1, MITOCHONDRIAL-RELATEDcoord: 30..77
coord: 94..228
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 1..142
IPR0089276-phosphogluconate dehydrogenase-like, C-terminal domain superfamilySUPERFAMILY481796-phosphogluconate dehydrogenase C-terminal domain-likecoord: 144..224

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy6G116440.1Chy6G116440.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051287 NAD binding
molecular_function GO:0050661 NADP binding
molecular_function GO:0016491 oxidoreductase activity