Chy6G116010 (gene) Cucumber (hystrix) v1

Overview
NameChy6G116010
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionDDE Tnp4 domain-containing protein
LocationchrH06: 8744296 .. 8745570 (-)
RNA-Seq ExpressionChy6G116010
SyntenyChy6G116010
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTCTCCTCGTTTAGCTGCTTTACTCTCTTCTTTGATCTCCCAACTCCTTCTCCTCCTCTTCCTCCTCTTCCCTTCCTCCAACCCACATTCCCTTTTCTCCAATTCCGCCCCCGATTCCAGTTTCTATGCCAATCTCTTCGCCCACTTCCTCTTTTCCCAGGATTTTGCCGCTTCCCTTCCCTTTCTCTCTGTTTCCCGCAAGAGGAAGAGAACCAATCGCTCCGACCATCTCGAATTGGGGTCATCCCATGGACGAGTTCATCATCTGTTTCGGACTCGGACTCCTGATTCTTTCAGAAATCACTTCAGAATGACTTCTTCAACGTTTGAATGGCTCTCTGGTTTGCTCGAGCCCCTTCTCGAGTGTCGTGACCCGGTGGGTTCTCCTCTCGATCTCTCCGTTGAGATTCGACTAGGTGTTGGTTTGTATCGCCTCGCCACTGGCTGCGATTTCTCCACAATCTCCGACCAATTTGGTATTTCGGAGTCTGTAGCGAGGTTCTGCTCTAAACAATTGTGTCGAGTTCTCTGTACTAATTTTCGCTTCTGGGTTGAATTCCCTTGCCCCAATGAGCTGGAATTAACATCCTCGGCTTTTGAAGATCTTGCTGGGCTTCCGAATTGCTGCGGTGTGGTTTCTTGTACAAGGTTCAAGATTATTAGAAATAGCCATTTTTATGAAGATAGCGTCGCAACTCAACTTGTTGTTGATTCTTCGTCACGAATCCTTAGTATTGTTGCAGGATTTCGTGGCAATAAGGACGACTCGACAGTGCTTATGTCCTCGACGCTGTTTAAAGACATTGAACAAGGAAGGCTTCTGAATTCTCCTCCGGTTTACCTTCATGGGGTGGCTGTGAATAAGTACTTGTTTGGACATGGTGAATATCCTTTGCTTCCATGGTTAATAGTGCCTTTTGCAGGAGCTGTTTCAGGGTCAACTGAAGAGAGTTTCAATGAAGCTCATCGATTGATGTGCATTCCAGCTCTGAAAGCAATTGTTAGTTTGAGAAATTGGGGAGTTTTGAGTCAACCAATTCATGAGGAGTTCAAAACTGCTGTTGCTTACATTGGTGCTTGCTCAATTCTTCATAATGCTTTGTTGATGAGGGAGGATTTTTCTGCCATGGCTGATGAGTGGGAGAGCTTATCTTCACTTGATCATAGATCTCAGTATGTTGAAGCTGGATTGAATGTGGATTCAACTAATGAGAAAGCTTCTGTTATACAGAGAGCATTGGCTCTGAGAGCTAGAGAGCTTCACAGTTAG

mRNA sequence

ATGGATTCTCCTCGTTTAGCTGCTTTACTCTCTTCTTTGATCTCCCAACTCCTTCTCCTCCTCTTCCTCCTCTTCCCTTCCTCCAACCCACATTCCCTTTTCTCCAATTCCGCCCCCGATTCCAGTTTCTATGCCAATCTCTTCGCCCACTTCCTCTTTTCCCAGGATTTTGCCGCTTCCCTTCCCTTTCTCTCTGTTTCCCGCAAGAGGAAGAGAACCAATCGCTCCGACCATCTCGAATTGGGGTCATCCCATGGACGAGTTCATCATCTGTTTCGGACTCGGACTCCTGATTCTTTCAGAAATCACTTCAGAATGACTTCTTCAACGTTTGAATGGCTCTCTGGTTTGCTCGAGCCCCTTCTCGAGTGTCGTGACCCGGTGGGTTCTCCTCTCGATCTCTCCGTTGAGATTCGACTAGGTGTTGGTTTGTATCGCCTCGCCACTGGCTGCGATTTCTCCACAATCTCCGACCAATTTGGTATTTCGGAGTCTGTAGCGAGGTTCTGCTCTAAACAATTGTGTCGAGTTCTCTGTACTAATTTTCGCTTCTGGGTTGAATTCCCTTGCCCCAATGAGCTGGAATTAACATCCTCGGCTTTTGAAGATCTTGCTGGGCTTCCGAATTGCTGCGGTGTGGTTTCTTGTACAAGGTTCAAGATTATTAGAAATAGCCATTTTTATGAAGATAGCGTCGCAACTCAACTTGTTGTTGATTCTTCGTCACGAATCCTTAGTATTGTTGCAGGATTTCGTGGCAATAAGGACGACTCGACAGTGCTTATGTCCTCGACGCTGTTTAAAGACATTGAACAAGGAAGGCTTCTGAATTCTCCTCCGGTTTACCTTCATGGGGTGGCTGTGAATAAGTACTTGTTTGGACATGGTGAATATCCTTTGCTTCCATGGTTAATAGTGCCTTTTGCAGGAGCTGTTTCAGGGTCAACTGAAGAGAGTTTCAATGAAGCTCATCGATTGATGTGCATTCCAGCTCTGAAAGCAATTGTTAGTTTGAGAAATTGGGGAGTTTTGAGTCAACCAATTCATGAGGAGTTCAAAACTGCTGTTGCTTACATTGGTGCTTGCTCAATTCTTCATAATGCTTTGTTGATGAGGGAGGATTTTTCTGCCATGGCTGATGAGTGGGAGAGCTTATCTTCACTTGATCATAGATCTCAGTATGTTGAAGCTGGATTGAATGTGGATTCAACTAATGAGAAAGCTTCTGTTATACAGAGAGCATTGGCTCTGAGAGCTAGAGAGCTTCACAGTTAG

Coding sequence (CDS)

ATGGATTCTCCTCGTTTAGCTGCTTTACTCTCTTCTTTGATCTCCCAACTCCTTCTCCTCCTCTTCCTCCTCTTCCCTTCCTCCAACCCACATTCCCTTTTCTCCAATTCCGCCCCCGATTCCAGTTTCTATGCCAATCTCTTCGCCCACTTCCTCTTTTCCCAGGATTTTGCCGCTTCCCTTCCCTTTCTCTCTGTTTCCCGCAAGAGGAAGAGAACCAATCGCTCCGACCATCTCGAATTGGGGTCATCCCATGGACGAGTTCATCATCTGTTTCGGACTCGGACTCCTGATTCTTTCAGAAATCACTTCAGAATGACTTCTTCAACGTTTGAATGGCTCTCTGGTTTGCTCGAGCCCCTTCTCGAGTGTCGTGACCCGGTGGGTTCTCCTCTCGATCTCTCCGTTGAGATTCGACTAGGTGTTGGTTTGTATCGCCTCGCCACTGGCTGCGATTTCTCCACAATCTCCGACCAATTTGGTATTTCGGAGTCTGTAGCGAGGTTCTGCTCTAAACAATTGTGTCGAGTTCTCTGTACTAATTTTCGCTTCTGGGTTGAATTCCCTTGCCCCAATGAGCTGGAATTAACATCCTCGGCTTTTGAAGATCTTGCTGGGCTTCCGAATTGCTGCGGTGTGGTTTCTTGTACAAGGTTCAAGATTATTAGAAATAGCCATTTTTATGAAGATAGCGTCGCAACTCAACTTGTTGTTGATTCTTCGTCACGAATCCTTAGTATTGTTGCAGGATTTCGTGGCAATAAGGACGACTCGACAGTGCTTATGTCCTCGACGCTGTTTAAAGACATTGAACAAGGAAGGCTTCTGAATTCTCCTCCGGTTTACCTTCATGGGGTGGCTGTGAATAAGTACTTGTTTGGACATGGTGAATATCCTTTGCTTCCATGGTTAATAGTGCCTTTTGCAGGAGCTGTTTCAGGGTCAACTGAAGAGAGTTTCAATGAAGCTCATCGATTGATGTGCATTCCAGCTCTGAAAGCAATTGTTAGTTTGAGAAATTGGGGAGTTTTGAGTCAACCAATTCATGAGGAGTTCAAAACTGCTGTTGCTTACATTGGTGCTTGCTCAATTCTTCATAATGCTTTGTTGATGAGGGAGGATTTTTCTGCCATGGCTGATGAGTGGGAGAGCTTATCTTCACTTGATCATAGATCTCAGTATGTTGAAGCTGGATTGAATGTGGATTCAACTAATGAGAAAGCTTCTGTTATACAGAGAGCATTGGCTCTGAGAGCTAGAGAGCTTCACAGTTAG

Protein sequence

MDSPRLAALLSSLISQLLLLLFLLFPSSNPHSLFSNSAPDSSFYANLFAHFLFSQDFAASLPFLSVSRKRKRTNRSDHLELGSSHGRVHHLFRTRTPDSFRNHFRMTSSTFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGISESVARFCSKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNSHFYEDSVATQLVVDSSSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLNSPPVYLHGVAVNKYLFGHGEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLSQPIHEEFKTAVAYIGACSILHNALLMREDFSAMADEWESLSSLDHRSQYVEAGLNVDSTNEKASVIQRALALRARELHS*
Homology
BLAST of Chy6G116010 vs. ExPASy Swiss-Prot
Match: Q9M2U3 (Protein ALP1-like OS=Arabidopsis thaliana OX=3702 GN=At3g55350 PE=2 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 9.6e-29
Identity = 86/303 (28.38%), Postives = 147/303 (48.51%), Query Frame = 0

Query: 97  PDSFRNHFRMTSSTFEWLSGLLEPLLECR-----DPVGSPLDLSVEIRLGVGLYRLATGC 156
           P +F + F+++  TF+++  L++     +     D  G+P  LS+  R+ V L RL +G 
Sbjct: 69  PKTFESVFKISRKTFDYICSLVKADFTAKPANFSDSNGNP--LSLNDRVAVALRRLGSGE 128

Query: 157 DFSTISDQFGISESVA-----RFCSKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAG 216
             S I + FG+++S       RF      R +  +   W     P++L+   S FE ++G
Sbjct: 129 SLSVIGETFGMNQSTVSQITWRFVESMEERAI--HHLSW-----PSKLDEIKSKFEKISG 188

Query: 217 LPNCCGVVSCTRFKIIRNSHFYED------------SVATQLVVDSSSRILSIVAGFRGN 276
           LPNCCG +  T   I+ N    E             S+  Q VVD   R L ++AG+ G+
Sbjct: 189 LPNCCGAIDITH--IVMNLPAVEPSNKVWLDGEKNFSMTLQAVVDPDMRFLDVIAGWPGS 248

Query: 277 KDDSTVLMSSTLFKDIEQGRLLNSPPVYL-HGVAVNKYLFGHGEYPLLPWLIVPFAGAVS 336
            +D  VL +S  +K +E+G+ LN   + L     + +Y+ G   +PLLPWL+ P+ G  +
Sbjct: 249 LNDDVVLKNSGFYKLVEKGKRLNGEKLPLSERTELREYIVGDSGFPLLPWLLTPYQGKPT 308

Query: 337 GSTEESFNEAHRLMCIPALKAIVSLRN-WGVLSQPIHEEFKTAV-AYIGACSILHNALLM 375
              +  FN+ H      A  A+  L++ W +++  +    +  +   I  C +LHN ++ 
Sbjct: 309 SLPQTEFNKRHSEATKAAQMALSKLKDRWRIINGVMWMPDRNRLPRIIFVCCLLHNIIID 360

BLAST of Chy6G116010 vs. ExPASy Swiss-Prot
Match: Q94K49 (Protein ANTAGONIST OF LIKE HETEROCHROMATIN PROTEIN 1 OS=Arabidopsis thaliana OX=3702 GN=ALP1 PE=1 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 4.0e-27
Identity = 84/293 (28.67%), Postives = 139/293 (47.44%), Query Frame = 0

Query: 99  SFRNHFRMTSSTFEWLSGLLEPLLECRDPVG----SPLDLSVEIRLGVGLYRLATGCDFS 158
           +F++ FR + +TF ++  L+   L  R P G        LSVE ++ + L RLA+G    
Sbjct: 64  AFKHFFRASKTTFSYICSLVREDLISRPPSGLINIEGRLLSVEKQVAIALRRLASGDSQV 123

Query: 159 TISDQFGISESVARFCSKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVV 218
           ++   FG+ +S     + +    L    +  + +P  + +E   S FE++ GLPNCCG +
Sbjct: 124 SVGAAFGVGQSTVSQVTWRFIEALEERAKHHLRWPDSDRIEEIKSKFEEMYGLPNCCGAI 183

Query: 219 SCTR----FKIIRNSHFYED-----SVATQLVVDSSSRILSIVAGFRGNKDDSTVLMSST 278
             T        ++ S  + D     S+  Q V D   R L++V G+ G    S +L  S 
Sbjct: 184 DTTHIIMTLPAVQASDDWCDQEKNYSMFLQGVFDHEMRFLNMVTGWPGGMTVSKLLKFSG 243

Query: 279 LFKDIEQGRLLNSPPVYL-HGVAVNKYLFGHGEYPLLPWLIVPFAGAVSGSTEESFNEAH 338
            FK  E  ++L+  P  L  G  + +Y+ G   YPLLPWLI P        +  +FNE H
Sbjct: 244 FFKLCENAQILDGNPKTLSQGAQIREYVVGGISYPLLPWLITPHDSDHPSDSMVAFNERH 303

Query: 339 RLMCIPALKAIVSLR-NWGVLSQPI-HEEFKTAVAYIGACSILHNALLMREDF 376
             +   A  A   L+ +W +LS+ +   + +   + I  C +LHN ++   D+
Sbjct: 304 EKVRSVAATAFQQLKGSWRILSKVMWRPDRRKLPSIILVCCLLHNIIIDCGDY 356

BLAST of Chy6G116010 vs. ExPASy TrEMBL
Match: A0A0A0LBX6 (DDE Tnp4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G202740 PE=3 SV=1)

HSP 1 Score: 847.8 bits (2189), Expect = 1.9e-242
Identity = 422/424 (99.53%), Postives = 424/424 (100.00%), Query Frame = 0

Query: 1   MDSPRLAALLSSLISQLLLLLFLLFPSSNPHSLFSNSAPDSSFYANLFAHFLFSQDFAAS 60
           MDSPRLAALLSSLISQLLLLLFLLFPSSNPHSLFSNSAPDSSFYANLFAHFLFSQDFAAS
Sbjct: 1   MDSPRLAALLSSLISQLLLLLFLLFPSSNPHSLFSNSAPDSSFYANLFAHFLFSQDFAAS 60

Query: 61  LPFLSVSRKRKRTNRSDHLELGSSHGRVHHLFRTRTPDSFRNHFRMTSSTFEWLSGLLEP 120
           LPFLSVSRKRKRTNRSDHLELGSSHGRVHHLFRTRTPDSFRNHFRMTSSTFEWLSGLLEP
Sbjct: 61  LPFLSVSRKRKRTNRSDHLELGSSHGRVHHLFRTRTPDSFRNHFRMTSSTFEWLSGLLEP 120

Query: 121 LLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGISESVARFCSKQLCRVLCT 180
           LLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFG+SESVARFCSKQLCRVLCT
Sbjct: 121 LLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGVSESVARFCSKQLCRVLCT 180

Query: 181 NFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNSHFYEDSVATQLVVDS 240
           NFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNSHFYEDSVATQLVVDS
Sbjct: 181 NFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNSHFYEDSVATQLVVDS 240

Query: 241 SSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLNSPPVYLHGVAVNKYLFGHGEYPL 300
           SSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLNSPPVYLHGVAVNKYLFGHGEYPL
Sbjct: 241 SSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLNSPPVYLHGVAVNKYLFGHGEYPL 300

Query: 301 LPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLSQPIHEEFKTAVAYIG 360
           LPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLSQPIHEEFKTAVAYIG
Sbjct: 301 LPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLSQPIHEEFKTAVAYIG 360

Query: 361 ACSILHNALLMREDFSAMADEWESLSSLDHRSQYVEAGLNVDSTNEKASVIQRALALRAR 420
           ACSILHNALLMREDFSAMADEWESLSSLDH+SQYVEAGLNVDSTNEKASVIQRALALRAR
Sbjct: 361 ACSILHNALLMREDFSAMADEWESLSSLDHKSQYVEAGLNVDSTNEKASVIQRALALRAR 420

Query: 421 ELHS 425
           ELHS
Sbjct: 421 ELHS 424

BLAST of Chy6G116010 vs. ExPASy TrEMBL
Match: A0A5D3CRB2 (Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold832G001410 PE=3 SV=1)

HSP 1 Score: 835.9 bits (2158), Expect = 7.6e-239
Identity = 417/424 (98.35%), Postives = 418/424 (98.58%), Query Frame = 0

Query: 1   MDSPRLAALLSSLISQLLLLLFLLFPSSNPHSLFSNSAPDSSFYANLFAHFLFSQDFAAS 60
           MDSPRLAALLSSLISQLLLLLFLLFPSSNPHSLFSNS PDSSFYANLF HFLFSQDFAAS
Sbjct: 1   MDSPRLAALLSSLISQLLLLLFLLFPSSNPHSLFSNSTPDSSFYANLFTHFLFSQDFAAS 60

Query: 61  LPFLSVSRKRKRTNRSDHLELGSSHGRVHHLFRTRTPDSFRNHFRMTSSTFEWLSGLLEP 120
           LPFLSVSRKRKRTN  DHLELGSSHGRVHHLFRTRTPDSFRNHFRMTSSTFEWLSGLLEP
Sbjct: 61  LPFLSVSRKRKRTNPPDHLELGSSHGRVHHLFRTRTPDSFRNHFRMTSSTFEWLSGLLEP 120

Query: 121 LLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGISESVARFCSKQLCRVLCT 180
           LLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFG+SESVARFCSKQLCRVLCT
Sbjct: 121 LLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGVSESVARFCSKQLCRVLCT 180

Query: 181 NFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNSHFYEDSVATQLVVDS 240
           NFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNSHFYEDSVATQLVVDS
Sbjct: 181 NFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNSHFYEDSVATQLVVDS 240

Query: 241 SSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLNSPPVYLHGVAVNKYLFGHGEYPL 300
           SSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLNSPPVYLHGVAVNKYLFG GEYPL
Sbjct: 241 SSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLNSPPVYLHGVAVNKYLFGRGEYPL 300

Query: 301 LPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLSQPIHEEFKTAVAYIG 360
           LPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLSQPIHEEFKTAVAYIG
Sbjct: 301 LPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLSQPIHEEFKTAVAYIG 360

Query: 361 ACSILHNALLMREDFSAMADEWESLSSLDHRSQYVEAGLNVDSTNEKASVIQRALALRAR 420
           ACSILHNALLMREDFSAMADEWESLSSLDHRSQYVEAGLNVDSTNEKASVIQRALA RAR
Sbjct: 361 ACSILHNALLMREDFSAMADEWESLSSLDHRSQYVEAGLNVDSTNEKASVIQRALAQRAR 420

Query: 421 ELHS 425
           ELHS
Sbjct: 421 ELHS 424

BLAST of Chy6G116010 vs. ExPASy TrEMBL
Match: A0A1S3C2M8 (uncharacterized protein LOC103496169 OS=Cucumis melo OX=3656 GN=LOC103496169 PE=3 SV=1)

HSP 1 Score: 740.0 bits (1909), Expect = 5.7e-210
Identity = 364/372 (97.85%), Postives = 366/372 (98.39%), Query Frame = 0

Query: 53  FSQDFAASLPFLSVSRKRKRTNRSDHLELGSSHGRVHHLFRTRTPDSFRNHFRMTSSTFE 112
           F + FAASLPFLSVSRKRKRTN  DHLELGSSHGRVHHLFRTRTPDSFRNHFRMTSSTFE
Sbjct: 10  FPRIFAASLPFLSVSRKRKRTNPPDHLELGSSHGRVHHLFRTRTPDSFRNHFRMTSSTFE 69

Query: 113 WLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGISESVARFCSK 172
           WLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFG+SESVARFCSK
Sbjct: 70  WLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGVSESVARFCSK 129

Query: 173 QLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNSHFYEDSV 232
           QLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNSHFYEDSV
Sbjct: 130 QLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNSHFYEDSV 189

Query: 233 ATQLVVDSSSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLNSPPVYLHGVAVNKYL 292
           ATQLVVDSSSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLNSPPVYLHGVAVNKYL
Sbjct: 190 ATQLVVDSSSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLNSPPVYLHGVAVNKYL 249

Query: 293 FGHGEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLSQPIHEEF 352
           FG GEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLSQPIHEEF
Sbjct: 250 FGRGEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLSQPIHEEF 309

Query: 353 KTAVAYIGACSILHNALLMREDFSAMADEWESLSSLDHRSQYVEAGLNVDSTNEKASVIQ 412
           KTAVAYIGACSILHNALLMREDFSAMADEWESLSSLDHRSQYVEAGLNVDSTNEKASVIQ
Sbjct: 310 KTAVAYIGACSILHNALLMREDFSAMADEWESLSSLDHRSQYVEAGLNVDSTNEKASVIQ 369

Query: 413 RALALRARELHS 425
           RALA RARELHS
Sbjct: 370 RALAQRARELHS 381

BLAST of Chy6G116010 vs. ExPASy TrEMBL
Match: A0A6J1FNZ2 (protein ALP1-like OS=Cucurbita moschata OX=3662 GN=LOC111446927 PE=3 SV=1)

HSP 1 Score: 670.6 bits (1729), Expect = 4.3e-189
Identity = 345/435 (79.31%), Postives = 373/435 (85.75%), Query Frame = 0

Query: 1   MDSPRLAALLSSLISQLLLLLFLLFPSSNPHSLFSNSAPDSSFYANLFA---HFLFSQDF 60
           MDS +LAALLSSLISQLLLLL LLFPSSNPHSL SNS+ DS+FYANLF    HFLFSQ  
Sbjct: 1   MDSRQLAALLSSLISQLLLLLLLLFPSSNPHSLLSNSSSDSNFYANLFPLFNHFLFSQQI 60

Query: 61  AASLPFLSVSRKRKRTNRSDHLELGSS--------HGRVHHLFRTRTPDSFRNHFRMTSS 120
           AASL FLSVSRKRKRT+ S+ LELG S         GRV HL RTR+PDSFRNHFRMTSS
Sbjct: 61  AASLSFLSVSRKRKRTHSSELLELGPSDSGGEDGGRGRV-HLLRTRSPDSFRNHFRMTSS 120

Query: 121 TFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGISESVARF 180
           TFEWLSGLLEPLLECRDPVGSPLDLS EIRLGVGL RLATGCDFSTISDQFG+SESVARF
Sbjct: 121 TFEWLSGLLEPLLECRDPVGSPLDLSAEIRLGVGLSRLATGCDFSTISDQFGVSESVARF 180

Query: 181 CSKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNSHFYE 240
           C+KQLCRVLCTNFRFWVEFPCP+ELELTSS+FED+AGLPNCCGV+SCT            
Sbjct: 181 CAKQLCRVLCTNFRFWVEFPCPSELELTSSSFEDIAGLPNCCGVISCT------------ 240

Query: 241 DSVATQLVVDSSSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLNSPPVYLHGVAVN 300
                           SIVAGFRG+KDDSTVLMS+TLFKDIE+ RLL SPPVYLHG+AVN
Sbjct: 241 ----------------SIVAGFRGDKDDSTVLMSTTLFKDIEEERLLGSPPVYLHGMAVN 300

Query: 301 KYLFGHGEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLSQPIH 360
           +YLFGHGEYPLLPWL+VPFAGAVSGSTEESFNEAHRLMCIPALKAI+SLRNWGVLSQP+H
Sbjct: 301 QYLFGHGEYPLLPWLMVPFAGAVSGSTEESFNEAHRLMCIPALKAIISLRNWGVLSQPMH 360

Query: 361 EEFKTAVAYIGACSILHNALLMREDFSAMADEWESLSSLDHRSQYVEAGLNVDSTNEKAS 420
           EEFKTAVAYIGACSILHNALLMREDF+AMADEWESL+SLDH SQYV  GLN DS +EKA+
Sbjct: 361 EEFKTAVAYIGACSILHNALLMREDFTAMADEWESLASLDHSSQYVGIGLNEDSPDEKAT 406

Query: 421 VIQRALALRARELHS 425
           ++Q+ALALRARELH+
Sbjct: 421 MLQKALALRARELHT 406

BLAST of Chy6G116010 vs. ExPASy TrEMBL
Match: A0A6J1J0M5 (protein ALP1-like OS=Cucurbita maxima OX=3661 GN=LOC111480245 PE=3 SV=1)

HSP 1 Score: 669.8 bits (1727), Expect = 7.3e-189
Identity = 347/435 (79.77%), Postives = 372/435 (85.52%), Query Frame = 0

Query: 1   MDSPRLAALLSSLISQLLLLLFLLFPSSNPHSLFSNSAPDSSFYANLFA---HFLFSQDF 60
           MDS +LAALLSSLISQLLLLL LLFPSSNPHSL SNS+ DS+FYANLF    HFLFSQ  
Sbjct: 1   MDSRQLAALLSSLISQLLLLLLLLFPSSNPHSLLSNSSSDSNFYANLFPLFNHFLFSQQI 60

Query: 61  AASLPFLSVSRKRKRTNRSDHLELGSS--------HGRVHHLFRTRTPDSFRNHFRMTSS 120
           AASL FLSVSRKRKRT+ S+ LELG S         GRV HL RTR+PDSFRNHFRMTSS
Sbjct: 61  AASLSFLSVSRKRKRTHSSELLELGPSDSGGEDGGRGRV-HLLRTRSPDSFRNHFRMTSS 120

Query: 121 TFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGISESVARF 180
           TFEWLSGLLEPLLECRDPVGSPLDLS EIRLGVGL RLATGCDFSTISDQFG+SESVARF
Sbjct: 121 TFEWLSGLLEPLLECRDPVGSPLDLSAEIRLGVGLSRLATGCDFSTISDQFGVSESVARF 180

Query: 181 CSKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNSHFYE 240
           C+KQLCRVLCTNFRFWVEFPCP+ELELTSSAFED+AGLPNCCGV+SCT            
Sbjct: 181 CAKQLCRVLCTNFRFWVEFPCPSELELTSSAFEDIAGLPNCCGVISCT------------ 240

Query: 241 DSVATQLVVDSSSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLNSPPVYLHGVAVN 300
                           SIVAGFRG+KDDSTVLMS+TLFKDIE+ RLL SPPVYLHGVAVN
Sbjct: 241 ----------------SIVAGFRGDKDDSTVLMSTTLFKDIEEERLLGSPPVYLHGVAVN 300

Query: 301 KYLFGHGEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLSQPIH 360
           +YLFGHG+YPLLPWL+VPFAGAVSGSTEESFNEAHRLM IPALKAI+SLRNWGVLSQP+H
Sbjct: 301 QYLFGHGDYPLLPWLMVPFAGAVSGSTEESFNEAHRLMSIPALKAIISLRNWGVLSQPMH 360

Query: 361 EEFKTAVAYIGACSILHNALLMREDFSAMADEWESLSSLDHRSQYVEAGLNVDSTNEKAS 420
           EEFKTAVAYIGACSILHNALLMREDF+AMADEWESL+SLDH SQYV  GLN DS +EKAS
Sbjct: 361 EEFKTAVAYIGACSILHNALLMREDFTAMADEWESLASLDHSSQYVGIGLNEDSPDEKAS 406

Query: 421 VIQRALALRARELHS 425
           +IQ+ALALRARELH+
Sbjct: 421 MIQKALALRARELHT 406

BLAST of Chy6G116010 vs. NCBI nr
Match: KGN57516.1 (hypothetical protein Csa_011580 [Cucumis sativus])

HSP 1 Score: 845 bits (2184), Expect = 2.86e-309
Identity = 422/424 (99.53%), Postives = 424/424 (100.00%), Query Frame = 0

Query: 1   MDSPRLAALLSSLISQLLLLLFLLFPSSNPHSLFSNSAPDSSFYANLFAHFLFSQDFAAS 60
           MDSPRLAALLSSLISQLLLLLFLLFPSSNPHSLFSNSAPDSSFYANLFAHFLFSQDFAAS
Sbjct: 1   MDSPRLAALLSSLISQLLLLLFLLFPSSNPHSLFSNSAPDSSFYANLFAHFLFSQDFAAS 60

Query: 61  LPFLSVSRKRKRTNRSDHLELGSSHGRVHHLFRTRTPDSFRNHFRMTSSTFEWLSGLLEP 120
           LPFLSVSRKRKRTNRSDHLELGSSHGRVHHLFRTRTPDSFRNHFRMTSSTFEWLSGLLEP
Sbjct: 61  LPFLSVSRKRKRTNRSDHLELGSSHGRVHHLFRTRTPDSFRNHFRMTSSTFEWLSGLLEP 120

Query: 121 LLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGISESVARFCSKQLCRVLCT 180
           LLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFG+SESVARFCSKQLCRVLCT
Sbjct: 121 LLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGVSESVARFCSKQLCRVLCT 180

Query: 181 NFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNSHFYEDSVATQLVVDS 240
           NFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNSHFYEDSVATQLVVDS
Sbjct: 181 NFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNSHFYEDSVATQLVVDS 240

Query: 241 SSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLNSPPVYLHGVAVNKYLFGHGEYPL 300
           SSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLNSPPVYLHGVAVNKYLFGHGEYPL
Sbjct: 241 SSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLNSPPVYLHGVAVNKYLFGHGEYPL 300

Query: 301 LPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLSQPIHEEFKTAVAYIG 360
           LPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLSQPIHEEFKTAVAYIG
Sbjct: 301 LPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLSQPIHEEFKTAVAYIG 360

Query: 361 ACSILHNALLMREDFSAMADEWESLSSLDHRSQYVEAGLNVDSTNEKASVIQRALALRAR 420
           ACSILHNALLMREDFSAMADEWESLSSLDH+SQYVEAGLNVDSTNEKASVIQRALALRAR
Sbjct: 361 ACSILHNALLMREDFSAMADEWESLSSLDHKSQYVEAGLNVDSTNEKASVIQRALALRAR 420

Query: 421 ELHS 424
           ELHS
Sbjct: 421 ELHS 424

BLAST of Chy6G116010 vs. NCBI nr
Match: KAA0037135.1 (putative nuclease HARBI1 [Cucumis melo var. makuwa] >TYK13940.1 putative nuclease HARBI1 [Cucumis melo var. makuwa])

HSP 1 Score: 833 bits (2153), Expect = 1.52e-304
Identity = 417/424 (98.35%), Postives = 418/424 (98.58%), Query Frame = 0

Query: 1   MDSPRLAALLSSLISQLLLLLFLLFPSSNPHSLFSNSAPDSSFYANLFAHFLFSQDFAAS 60
           MDSPRLAALLSSLISQLLLLLFLLFPSSNPHSLFSNS PDSSFYANLF HFLFSQDFAAS
Sbjct: 1   MDSPRLAALLSSLISQLLLLLFLLFPSSNPHSLFSNSTPDSSFYANLFTHFLFSQDFAAS 60

Query: 61  LPFLSVSRKRKRTNRSDHLELGSSHGRVHHLFRTRTPDSFRNHFRMTSSTFEWLSGLLEP 120
           LPFLSVSRKRKRTN  DHLELGSSHGRVHHLFRTRTPDSFRNHFRMTSSTFEWLSGLLEP
Sbjct: 61  LPFLSVSRKRKRTNPPDHLELGSSHGRVHHLFRTRTPDSFRNHFRMTSSTFEWLSGLLEP 120

Query: 121 LLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGISESVARFCSKQLCRVLCT 180
           LLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFG+SESVARFCSKQLCRVLCT
Sbjct: 121 LLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGVSESVARFCSKQLCRVLCT 180

Query: 181 NFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNSHFYEDSVATQLVVDS 240
           NFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNSHFYEDSVATQLVVDS
Sbjct: 181 NFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNSHFYEDSVATQLVVDS 240

Query: 241 SSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLNSPPVYLHGVAVNKYLFGHGEYPL 300
           SSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLNSPPVYLHGVAVNKYLFG GEYPL
Sbjct: 241 SSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLNSPPVYLHGVAVNKYLFGRGEYPL 300

Query: 301 LPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLSQPIHEEFKTAVAYIG 360
           LPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLSQPIHEEFKTAVAYIG
Sbjct: 301 LPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLSQPIHEEFKTAVAYIG 360

Query: 361 ACSILHNALLMREDFSAMADEWESLSSLDHRSQYVEAGLNVDSTNEKASVIQRALALRAR 420
           ACSILHNALLMREDFSAMADEWESLSSLDHRSQYVEAGLNVDSTNEKASVIQRALA RAR
Sbjct: 361 ACSILHNALLMREDFSAMADEWESLSSLDHRSQYVEAGLNVDSTNEKASVIQRALAQRAR 420

Query: 421 ELHS 424
           ELHS
Sbjct: 421 ELHS 424

BLAST of Chy6G116010 vs. NCBI nr
Match: XP_008456140.1 (PREDICTED: uncharacterized protein LOC103496169 [Cucumis melo])

HSP 1 Score: 738 bits (1905), Expect = 1.86e-267
Identity = 364/372 (97.85%), Postives = 366/372 (98.39%), Query Frame = 0

Query: 53  FSQDFAASLPFLSVSRKRKRTNRSDHLELGSSHGRVHHLFRTRTPDSFRNHFRMTSSTFE 112
           F + FAASLPFLSVSRKRKRTN  DHLELGSSHGRVHHLFRTRTPDSFRNHFRMTSSTFE
Sbjct: 10  FPRIFAASLPFLSVSRKRKRTNPPDHLELGSSHGRVHHLFRTRTPDSFRNHFRMTSSTFE 69

Query: 113 WLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGISESVARFCSK 172
           WLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFG+SESVARFCSK
Sbjct: 70  WLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGVSESVARFCSK 129

Query: 173 QLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNSHFYEDSV 232
           QLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNSHFYEDSV
Sbjct: 130 QLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNSHFYEDSV 189

Query: 233 ATQLVVDSSSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLNSPPVYLHGVAVNKYL 292
           ATQLVVDSSSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLNSPPVYLHGVAVNKYL
Sbjct: 190 ATQLVVDSSSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLNSPPVYLHGVAVNKYL 249

Query: 293 FGHGEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLSQPIHEEF 352
           FG GEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLSQPIHEEF
Sbjct: 250 FGRGEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLSQPIHEEF 309

Query: 353 KTAVAYIGACSILHNALLMREDFSAMADEWESLSSLDHRSQYVEAGLNVDSTNEKASVIQ 412
           KTAVAYIGACSILHNALLMREDFSAMADEWESLSSLDHRSQYVEAGLNVDSTNEKASVIQ
Sbjct: 310 KTAVAYIGACSILHNALLMREDFSAMADEWESLSSLDHRSQYVEAGLNVDSTNEKASVIQ 369

Query: 413 RALALRARELHS 424
           RALA RARELHS
Sbjct: 370 RALAQRARELHS 381

BLAST of Chy6G116010 vs. NCBI nr
Match: XP_038880641.1 (protein ANTAGONIST OF LIKE HETEROCHROMATIN PROTEIN 1-like [Benincasa hispida])

HSP 1 Score: 732 bits (1889), Expect = 8.97e-265
Identity = 368/424 (86.79%), Postives = 385/424 (90.80%), Query Frame = 0

Query: 1   MDSPRLAALLSSLISQLLLLLFLLFPSSNPHSLFSNSAPDSSFYANLFAHFLFSQDFAAS 60
           MDSP+LAALLSSLISQLLLLLFLLFPSSNPHSLFSNS PDS+FYANLF HFLFSQDFAAS
Sbjct: 1   MDSPQLAALLSSLISQLLLLLFLLFPSSNPHSLFSNSTPDSNFYANLFTHFLFSQDFAAS 60

Query: 61  LPFLSVSRKRKRTNRSDHLELGSSHGRVHHLFRTRTPDSFRNHFRMTSSTFEWLSGLLEP 120
           LPFLSVSRKRKRTN SDHLELGSSHGR  HLFRTRTPDSFRNHFRMTSSTFEWLSGLLEP
Sbjct: 61  LPFLSVSRKRKRTNPSDHLELGSSHGRFDHLFRTRTPDSFRNHFRMTSSTFEWLSGLLEP 120

Query: 121 LLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGISESVARFCSKQLCRVLCT 180
           LLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFS IS QFG+SESVARFC+KQLCRVLCT
Sbjct: 121 LLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSKISHQFGVSESVARFCAKQLCRVLCT 180

Query: 181 NFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNSHFYEDSVATQLVVDS 240
           NFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVV+CT                       
Sbjct: 181 NFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVTCT----------------------- 240

Query: 241 SSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLNSPPVYLHGVAVNKYLFGHGEYPL 300
                SIVAGFRG+KDDSTVLMSSTLFKDIEQGRLL++PPVYLHGVAVN+YLFGHGEYPL
Sbjct: 241 -----SIVAGFRGDKDDSTVLMSSTLFKDIEQGRLLDAPPVYLHGVAVNQYLFGHGEYPL 300

Query: 301 LPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLSQPIHEEFKTAVAYIG 360
           LPWL++PFAGAVSGSTEESFN+AHRLMCIPALKAIVSLRNWGVLS+P+HEEFKTAVAYIG
Sbjct: 301 LPWLMLPFAGAVSGSTEESFNKAHRLMCIPALKAIVSLRNWGVLSKPMHEEFKTAVAYIG 360

Query: 361 ACSILHNALLMREDFSAMADEWESLSSLDHRSQYVEAGLNVDSTNEKASVIQRALALRAR 420
           ACSILHNALLMREDFSAMADEWESL+S DHRSQYVE  LN DSTNEKAS+IQRALA+RAR
Sbjct: 361 ACSILHNALLMREDFSAMADEWESLASFDHRSQYVEDKLNEDSTNEKASIIQRALAVRAR 396

Query: 421 ELHS 424
           ELHS
Sbjct: 421 ELHS 396

BLAST of Chy6G116010 vs. NCBI nr
Match: XP_023536005.1 (protein ALP1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 674 bits (1739), Expect = 9.16e-242
Identity = 349/435 (80.23%), Postives = 374/435 (85.98%), Query Frame = 0

Query: 1   MDSPRLAALLSSLISQLLLLLFLLFPSSNPHSLFSNSAPDSSFYANLFA---HFLFSQDF 60
           MDS +LAALLSSLISQLLLLL LLFPSSNPHSL SNS+ DS+FYANLF    HFLFSQ  
Sbjct: 1   MDSRQLAALLSSLISQLLLLLLLLFPSSNPHSLLSNSSSDSNFYANLFPLFNHFLFSQQI 60

Query: 61  AASLPFLSVSRKRKRTNRSDHLELGSS--------HGRVHHLFRTRTPDSFRNHFRMTSS 120
           AASL FLSVSRKRKRT+ S+ LELG S         GRVH L RTR+PDSFRNHFRMTSS
Sbjct: 61  AASLSFLSVSRKRKRTHSSELLELGPSDSGGEDGGRGRVH-LLRTRSPDSFRNHFRMTSS 120

Query: 121 TFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGISESVARF 180
           TFEWLSGLLEPLLECRDPVGSPLDLS EIRLGVGL RLATGCDFSTISDQFG+SESVARF
Sbjct: 121 TFEWLSGLLEPLLECRDPVGSPLDLSAEIRLGVGLSRLATGCDFSTISDQFGVSESVARF 180

Query: 181 CSKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNSHFYE 240
           C+KQLCRVLCTNFRFWVEFPCP+ELELTSSAFED+AGLPNCCGV+SCT            
Sbjct: 181 CAKQLCRVLCTNFRFWVEFPCPSELELTSSAFEDIAGLPNCCGVISCT------------ 240

Query: 241 DSVATQLVVDSSSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLNSPPVYLHGVAVN 300
                           SIVAGFRG+KDDSTVLMS+TLFKDIE+GRLL SPPVYLHG+AVN
Sbjct: 241 ----------------SIVAGFRGDKDDSTVLMSTTLFKDIEEGRLLGSPPVYLHGMAVN 300

Query: 301 KYLFGHGEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLSQPIH 360
           +YLFGHGEYPLLPWL+VPFAGAVSGSTEESFNEAHRLMCIPALKAI+SLRNWGVLSQP+H
Sbjct: 301 QYLFGHGEYPLLPWLMVPFAGAVSGSTEESFNEAHRLMCIPALKAIISLRNWGVLSQPMH 360

Query: 361 EEFKTAVAYIGACSILHNALLMREDFSAMADEWESLSSLDHRSQYVEAGLNVDSTNEKAS 420
           EEFKTAVAYIGACSILHNALLMREDF+AMADEWESL+SLDH SQYV  GLN DS +EKAS
Sbjct: 361 EEFKTAVAYIGACSILHNALLMREDFTAMADEWESLASLDHSSQYVGIGLNEDSPDEKAS 406

Query: 421 VIQRALALRARELHS 424
           +IQ+ALALRARELH+
Sbjct: 421 MIQKALALRARELHT 406

BLAST of Chy6G116010 vs. TAIR 10
Match: AT3G55350.1 (PIF / Ping-Pong family of plant transposases )

HSP 1 Score: 129.4 bits (324), Expect = 6.8e-30
Identity = 86/303 (28.38%), Postives = 147/303 (48.51%), Query Frame = 0

Query: 97  PDSFRNHFRMTSSTFEWLSGLLEPLLECR-----DPVGSPLDLSVEIRLGVGLYRLATGC 156
           P +F + F+++  TF+++  L++     +     D  G+P  LS+  R+ V L RL +G 
Sbjct: 69  PKTFESVFKISRKTFDYICSLVKADFTAKPANFSDSNGNP--LSLNDRVAVALRRLGSGE 128

Query: 157 DFSTISDQFGISESVA-----RFCSKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAG 216
             S I + FG+++S       RF      R +  +   W     P++L+   S FE ++G
Sbjct: 129 SLSVIGETFGMNQSTVSQITWRFVESMEERAI--HHLSW-----PSKLDEIKSKFEKISG 188

Query: 217 LPNCCGVVSCTRFKIIRNSHFYED------------SVATQLVVDSSSRILSIVAGFRGN 276
           LPNCCG +  T   I+ N    E             S+  Q VVD   R L ++AG+ G+
Sbjct: 189 LPNCCGAIDITH--IVMNLPAVEPSNKVWLDGEKNFSMTLQAVVDPDMRFLDVIAGWPGS 248

Query: 277 KDDSTVLMSSTLFKDIEQGRLLNSPPVYL-HGVAVNKYLFGHGEYPLLPWLIVPFAGAVS 336
            +D  VL +S  +K +E+G+ LN   + L     + +Y+ G   +PLLPWL+ P+ G  +
Sbjct: 249 LNDDVVLKNSGFYKLVEKGKRLNGEKLPLSERTELREYIVGDSGFPLLPWLLTPYQGKPT 308

Query: 337 GSTEESFNEAHRLMCIPALKAIVSLRN-WGVLSQPIHEEFKTAV-AYIGACSILHNALLM 375
              +  FN+ H      A  A+  L++ W +++  +    +  +   I  C +LHN ++ 
Sbjct: 309 SLPQTEFNKRHSEATKAAQMALSKLKDRWRIINGVMWMPDRNRLPRIIFVCCLLHNIIID 360

BLAST of Chy6G116010 vs. TAIR 10
Match: AT3G63270.1 (CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (InterPro:IPR006912); BEST Arabidopsis thaliana protein match is: PIF / Ping-Pong family of plant transposases (TAIR:AT3G55350.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 124.0 bits (310), Expect = 2.9e-28
Identity = 84/293 (28.67%), Postives = 139/293 (47.44%), Query Frame = 0

Query: 99  SFRNHFRMTSSTFEWLSGLLEPLLECRDPVG----SPLDLSVEIRLGVGLYRLATGCDFS 158
           +F++ FR + +TF ++  L+   L  R P G        LSVE ++ + L RLA+G    
Sbjct: 64  AFKHFFRASKTTFSYICSLVREDLISRPPSGLINIEGRLLSVEKQVAIALRRLASGDSQV 123

Query: 159 TISDQFGISESVARFCSKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVV 218
           ++   FG+ +S     + +    L    +  + +P  + +E   S FE++ GLPNCCG +
Sbjct: 124 SVGAAFGVGQSTVSQVTWRFIEALEERAKHHLRWPDSDRIEEIKSKFEEMYGLPNCCGAI 183

Query: 219 SCTR----FKIIRNSHFYED-----SVATQLVVDSSSRILSIVAGFRGNKDDSTVLMSST 278
             T        ++ S  + D     S+  Q V D   R L++V G+ G    S +L  S 
Sbjct: 184 DTTHIIMTLPAVQASDDWCDQEKNYSMFLQGVFDHEMRFLNMVTGWPGGMTVSKLLKFSG 243

Query: 279 LFKDIEQGRLLNSPPVYL-HGVAVNKYLFGHGEYPLLPWLIVPFAGAVSGSTEESFNEAH 338
            FK  E  ++L+  P  L  G  + +Y+ G   YPLLPWLI P        +  +FNE H
Sbjct: 244 FFKLCENAQILDGNPKTLSQGAQIREYVVGGISYPLLPWLITPHDSDHPSDSMVAFNERH 303

Query: 339 RLMCIPALKAIVSLR-NWGVLSQPI-HEEFKTAVAYIGACSILHNALLMREDF 376
             +   A  A   L+ +W +LS+ +   + +   + I  C +LHN ++   D+
Sbjct: 304 EKVRSVAATAFQQLKGSWRILSKVMWRPDRRKLPSIILVCCLLHNIIIDCGDY 356

BLAST of Chy6G116010 vs. TAIR 10
Match: AT4G29780.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G12010.1); Has 945 Blast hits to 944 proteins in 87 species: Archae - 0; Bacteria - 0; Metazoa - 519; Fungi - 43; Plants - 365; Viruses - 0; Other Eukaryotes - 18 (source: NCBI BLink). )

HSP 1 Score: 96.3 bits (238), Expect = 6.4e-20
Identity = 78/302 (25.83%), Postives = 128/302 (42.38%), Query Frame = 0

Query: 98  DSFRNHFRMTSSTFEWLSGLLEPLLE-----CRDPVGSPLDLSVEIRLGVGLYRLATGCD 157
           D FR  FRM+ STF  +   L+  +       RD + +P       R+GV ++RLATG  
Sbjct: 211 DEFRREFRMSKSTFNLICEELDTTVTKKNTMLRDAIPAPK------RVGVCVWRLATGAP 270

Query: 158 FSTISDQFGISESVARFCSKQLCR----VLCTNFRFWVEFPCPNELELTSSAFEDLAGLP 217
              +S++FG+  S       ++CR    VL   +  W   P  +E+  T + FE +  +P
Sbjct: 271 LRHVSERFGLGISTCHKLVIEVCRAIYDVLMPKYLLW---PSDSEINSTKAKFESVHKIP 330

Query: 218 NCCGVVSCTRFKIIR---------NSHFYED------SVATQLVVDSSSRILSIVAGFRG 277
           N  G +  T   II          N    E       S+  Q VV++      +  G  G
Sbjct: 331 NVVGSIYTTHIPIIAPKVHVAAYFNKRHTERNQKTSYSITVQGVVNADGIFTDVCIGNPG 390

Query: 278 NKDDSTVLMSSTLFKDIEQGRLLNSPPVYLHGVAVNKYLFGHGEYPLLPWLIVPFAGAVS 337
           +  D  +L  S+L           S      G+  + ++ G+  +PL  +L+VP+     
Sbjct: 391 SLTDDQILEKSSL-----------SRQRAARGMLRDSWIVGNSGFPLTDYLLVPYTRQNL 450

Query: 338 GSTEESFNEAHRLMCIPALKAIVSLR-NWGVLSQPIHEEFKTAVAYIGACSILHNALLMR 375
             T+ +FNE+   +   A  A   L+  W  L +    + +     +GAC +LHN   MR
Sbjct: 451 TWTQHAFNESIGEIQGIATAAFERLKGRWACLQKRTEVKLQDLPYVLGACCVLHNICEMR 492

BLAST of Chy6G116010 vs. TAIR 10
Match: AT3G19120.1 (PIF / Ping-Pong family of plant transposases )

HSP 1 Score: 90.9 bits (224), Expect = 2.7e-18
Identity = 95/392 (24.23%), Postives = 164/392 (41.84%), Query Frame = 0

Query: 12  SLISQLLLLLFLLFPSSNPHSLFSNSAPDSSFYANLF----AHFLFSQDFAASLPFLSVS 71
           +++S LL L   L P+S   S  S S+  S+  ++L     A  L     A+ L FL+V+
Sbjct: 7   AMLSHLLHLQNSLDPTSTLFSSASTSSQSSTTPSSLLSTSSAAPLLFFTLASLLSFLAVN 66

Query: 72  RKRKRTNRSDHLELGSSHGRVHH------LFRTRTPD------------SFRNHFRMTSS 131
           R    ++ S      S    +         FR  T D             +R+ + ++  
Sbjct: 67  RSSTESSSSSESPSPSPPPPLADGDYSVAAFRALTTDHIWSLDAPLRDARWRSLYGLSYP 126

Query: 132 TFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGISESVARF 191
            F  +   L+P +       S L L  +  + + L RLA GC   T++ ++ +   +   
Sbjct: 127 VFITVVDKLKPFI-----TASNLSLPADYAVAMVLSRLAHGCSAKTLASRYSLDPYLISK 186

Query: 192 CSKQLCRVLCTN-FRFWVEFPC-PNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNS-- 251
            +  + R+L T  +  +++ P     L  T+  FE+L  LPN CG +  T  K+ R +  
Sbjct: 187 ITNMVTRLLATKLYPEFIKIPVGKRRLIETTQGFEELTSLPNICGAIDSTPVKLRRRTKL 246

Query: 252 --------HFYEDSVATQLVVDSSSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLN 311
                    +  D+V  Q+V D       +     G +DDS+    S L+K +  G ++ 
Sbjct: 247 NPRNIYGCKYGYDAVLLQVVADHKKIFWDVCVKAPGGEDDSSHFRDSLLYKRLTSGDIVW 306

Query: 312 SPPVYLHGVAVNKYLFGHGEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVS 368
              + + G  V  Y+ G   YPLL +L+ PF+   SG+  E+  +   +     +   + 
Sbjct: 307 EKVINIRGHHVRPYIVGDWCYPLLSFLMTPFSPNGSGTPPENLFDGMLMKGRSVVVEAIG 366

BLAST of Chy6G116010 vs. TAIR 10
Match: AT1G72270.1 (CONTAINS InterPro DOMAIN/s: Ribosome 60S biogenesis N-terminal (InterPro:IPR021714); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G27010.1); Has 772 Blast hits to 657 proteins in 120 species: Archae - 0; Bacteria - 0; Metazoa - 344; Fungi - 94; Plants - 322; Viruses - 0; Other Eukaryotes - 12 (source: NCBI BLink). )

HSP 1 Score: 79.3 bits (194), Expect = 8.1e-15
Identity = 80/289 (27.68%), Postives = 120/289 (41.52%), Query Frame = 0

Query: 103 HFRMTSSTFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGI 162
           +FRM+ STF  L  +L                S        ++RLA G  +  +  +FG 
Sbjct: 100 YFRMSKSTFFSLYSILSH--------------SSLPSFAATIFRLAHGASYECLVHRFGF 159

Query: 163 -SESVARFCSKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKI 222
            S S A      +C+++       ++ P P+        F     LPNC GVV   RF++
Sbjct: 160 DSTSQASRSFFTVCKLINEKLSQQLDDPKPD--------FSPNL-LPNCYGVVGFGRFEV 219

Query: 223 IRNSHFYEDSVATQLVVDSSSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLNSPPV 282
                  + S+  Q +VDS+ R + I AG+        +   + LF   E+  +L+  P 
Sbjct: 220 KGKLLGAKGSILVQALVDSNGRFVDISAGWPSTMKPEAIFRQTKLFSIAEE--VLSGAPT 279

Query: 283 YL-HGVAVNKYLFGHGEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALK----AIV 342
            L +GV V +Y+ G    PLLPWL+ P+      S EESF E    +    L     A  
Sbjct: 280 KLGNGVLVPRYILGDSCLPLLPWLVTPYD---LTSDEESFREEFNNVVHTGLHSVEIAFA 339

Query: 343 SLR-NWGVLS---QPIHEEFKTAVAYIGACSILHNALLMREDFSAMADE 382
            +R  W +L    +P   EF   V   G   +LHN L+   D     +E
Sbjct: 340 KVRARWRILDKKWKPETIEFMPFVITTGC--LLHNFLVNSGDDDDSVEE 358

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M2U39.6e-2928.38Protein ALP1-like OS=Arabidopsis thaliana OX=3702 GN=At3g55350 PE=2 SV=1[more]
Q94K494.0e-2728.67Protein ANTAGONIST OF LIKE HETEROCHROMATIN PROTEIN 1 OS=Arabidopsis thaliana OX=... [more]
Match NameE-valueIdentityDescription
A0A0A0LBX61.9e-24299.53DDE Tnp4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G202740 PE... [more]
A0A5D3CRB27.6e-23998.35Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A1S3C2M85.7e-21097.85uncharacterized protein LOC103496169 OS=Cucumis melo OX=3656 GN=LOC103496169 PE=... [more]
A0A6J1FNZ24.3e-18979.31protein ALP1-like OS=Cucurbita moschata OX=3662 GN=LOC111446927 PE=3 SV=1[more]
A0A6J1J0M57.3e-18979.77protein ALP1-like OS=Cucurbita maxima OX=3661 GN=LOC111480245 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
KGN57516.12.86e-30999.53hypothetical protein Csa_011580 [Cucumis sativus][more]
KAA0037135.11.52e-30498.35putative nuclease HARBI1 [Cucumis melo var. makuwa] >TYK13940.1 putative nucleas... [more]
XP_008456140.11.86e-26797.85PREDICTED: uncharacterized protein LOC103496169 [Cucumis melo][more]
XP_038880641.18.97e-26586.79protein ANTAGONIST OF LIKE HETEROCHROMATIN PROTEIN 1-like [Benincasa hispida][more]
XP_023536005.19.16e-24280.23protein ALP1-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G55350.16.8e-3028.38PIF / Ping-Pong family of plant transposases [more]
AT3G63270.12.9e-2828.67CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (Int... [more]
AT4G29780.16.4e-2025.83unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT3G19120.12.7e-1824.23PIF / Ping-Pong family of plant transposases [more]
AT1G72270.18.1e-1527.68CONTAINS InterPro DOMAIN/s: Ribosome 60S biogenesis N-terminal (InterPro:IPR0217... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027806Harbinger transposase-derived nuclease domainPFAMPF13359DDE_Tnp_4coord: 227..367
e-value: 2.5E-12
score: 46.7
NoneNo IPR availablePANTHERPTHR22930UNCHARACTERIZEDcoord: 1..423
NoneNo IPR availablePANTHERPTHR22930:SF190OS06G0164500 PROTEINcoord: 1..423

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy6G116010.1Chy6G116010.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0046872 metal ion binding