Chy6G115550 (gene) Cucumber (hystrix) v1

Overview


Name	Chy6G115550
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Ribonuclease H
Location	chrH06: 8043622 .. 8044585 (+)
RNA-Seq Expression	Chy6G115550
Synteny	Chy6G115550

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAACCAAAGGCGTCGAATGGTCATCGATTCATTTTAGTAGCCATAGATTATTTTACTAAATGGGTGGAAGCTACATCGTACAAGAGTGTCACCAAGCAAGCTGTTGTCAAATTCATACAGAAGGATATTATATGTCGGTACGGCCTTCCTGAACGTATAATCACCGATAATGCCAAGAACTTAAACAATAAGTTAATGGAGGATTTATATAATCAGTTTAAAATTAAGCACTCCAATTCTACCCCGTACCACCCTAAGATGAATGGAGTGGTAGAGGCGGAAAATAAAAATATCAAAAGAATTATTGAAAAGATGACTATCACGTATAAAGATTGGCATGAGATGTTACCATTTGCATTACACGGTTACCGAACGTCAGTTCGCACATCAACTGGGGCAACTCCTTTCTCTTTAGTGTATGGCGTGGAAGCTGTTTTGCCTATTGAGGTCGAGGTGCCATCCCTCAAGGTAATCAAAGAAGCAGAACTAGAAGAAGCTGAATGGACACGGGCAAGGTACGAGGAATTAAACCTCATAGAAGAGAAAAGGTTGACTGCGTTGTGTAGAGGACAACTTTATCATAAGAAAATGACTCGTGCATACGATAGGAAAGTTTGACAACGTTGTTTTCAGGAAGGGGATTTGGTCTTAAAAAGGATCCTGCCATTTCAAAAAGATCATAAAGGAAAGTGGACCCCTAATTATGAGGGACCATACGTAGTAAAAAAGGAGTTCTCTGGTGGAGGTTTGATCCTCACAGGCATGGATGAGATTGATTTGCCTAACCCATTAAGTGCAGATTTTGTGAAGAAATACTATGCATGAAAATAGCAATAGTTTCCTCCGATTTTTGTTTATCCGGGCTTTTACCTCTTTGCACTGTTCTAAAAGGATCGTATCAATGCGTATCAAATTTGTGACAAACCTTGCAGAAACTAATGTTACTCAGGGATTCACATGA

mRNA sequence

ATGGAACCAAAGGCGTCGAATGGTCATCGATTCATTTTAGTAGCCATAGATTATTTTACTAAATGGGTGGAAGCTACATCGTACAAGAGTGTCACCAAGCAAGCTGTTGTCAAATTCATACAGAAGGATATTATATGTCGGTACGGCCTTCCTGAACGTATAATCACCGATAATGCCAAGAACTTAAACAATAAGTTAATGGAGGATTTATATAATCAGTTTAAAATTAAGCACTCCAATTCTACCCCGTACCACCCTAAGATGAATGGAGTGGTAGAGGCGGAAAATAAAAATATCAAAAGAATTATTGAAAAGATGACTATCACGTATAAAGATTGGCATGAGATGTTACCATTTGCATTACACGGTTACCGAACGTCAGTTCGCACATCAACTGGGGCAACTCCTTTCTCTTTAGTGTATGGCGTGGAAGCTGTTTTGCCTATTGAGGTCGAGGTGCCATCCCTCAAGGTAATCAAAGAAGCAGAACTAGAAGAAGCTGAATGGACACGGGCAAGGATCGTATCAATGCGTATCAAATTTGTGACAAACCTTGCAGAAACTAATGTTACTCAGGGATTCACATGA

Coding sequence (CDS)

ATGGAACCAAAGGCGTCGAATGGTCATCGATTCATTTTAGTAGCCATAGATTATTTTACTAAATGGGTGGAAGCTACATCGTACAAGAGTGTCACCAAGCAAGCTGTTGTCAAATTCATACAGAAGGATATTATATGTCGGTACGGCCTTCCTGAACGTATAATCACCGATAATGCCAAGAACTTAAACAATAAGTTAATGGAGGATTTATATAATCAGTTTAAAATTAAGCACTCCAATTCTACCCCGTACCACCCTAAGATGAATGGAGTGGTAGAGGCGGAAAATAAAAATATCAAAAGAATTATTGAAAAGATGACTATCACGTATAAAGATTGGCATGAGATGTTACCATTTGCATTACACGGTTACCGAACGTCAGTTCGCACATCAACTGGGGCAACTCCTTTCTCTTTAGTGTATGGCGTGGAAGCTGTTTTGCCTATTGAGGTCGAGGTGCCATCCCTCAAGGTAATCAAAGAAGCAGAACTAGAAGAAGCTGAATGGACACGGGCAAGGATCGTATCAATGCGTATCAAATTTGTGACAAACCTTGCAGAAACTAATGTTACTCAGGGATTCACATGA

Protein sequence

MEPKASNGHRFILVAIDYFTKWVEATSYKSVTKQAVVKFIQKDIICRYGLPERIITDNAKNLNNKLMEDLYNQFKIKHSNSTPYHPKMNGVVEAENKNIKRIIEKMTITYKDWHEMLPFALHGYRTSVRTSTGATPFSLVYGVEAVLPIEVEVPSLKVIKEAELEEAEWTRARIVSMRIKFVTNLAETNVTQGFT*

Homology

[Close]

BLAST of Chy6G115550 vs. ExPASy Swiss-Prot
Match: P03360 (Gag-Pol polyprotein (Fragment) OS=Avian reticuloendotheliosis virus OX=11636 GN=pol PE=1 SV=2)

HSP 1 Score: 85.9 bits (211), Expect = 5.6e-16
Identity = 50/141 (35.46%), Postives = 76/141 (53.90%), Query Frame = 0


Query: 5    ASNGHRFILVAIDYFTKWVEATSYKSVTKQAVVKFIQKDIICRYGLPERIITDNAKNLNN 64
            A  G++++LV +D F+ WVEA   K  T Q V+K +  DII R+GLP +I +DN      
Sbjct: 881  AKGGYKYLLVLVDTFSGWVEAYPAKRETSQVVIKHLIHDIIPRFGLPVQIGSDNGPAFVA 940

Query: 65   KLMEDLYNQFKIKHSNSTPYHPKMNGVVEAENKNIKRIIEKMTI-TYKDWHEMLPFALHG 124
            K+ + L     +       Y P+ +G VE  N+ +K  I K+ I T  DW  +LP AL  
Sbjct: 941  KVTQQLCEALNVSWKLHCAYRPQSSGQVERMNRTLKETIAKLRIETGGDWVSLLPQALLR 1000

Query: 125  YRTSVRTSTGATPFSLVYGVE 145
             R +     G +PF ++YG++
Sbjct: 1001 ARCTPGRE-GLSPFEILYGLK 1020

[Close]

BLAST of Chy6G115550 vs. ExPASy Swiss-Prot
Match: P26809 (Gag-Pol polyprotein OS=Friend murine leukemia virus (isolate FB29) OX=11797 GN=pol PE=3 SV=2)

HSP 1 Score: 79.7 bits (195), Expect = 4.0e-14
Identity = 44/137 (32.12%), Postives = 76/137 (55.47%), Query Frame = 0


Query: 8    GHRFILVAIDYFTKWVEATSYKSVTKQAVVKFIQKDIICRYGLPERIITDNAKNLNNKLM 67
            G++++LV ID F+ WVEA   K  T + V K + ++I  R+G+P+ + TDN     +K+ 
Sbjct: 1465 GYKYLLVFIDTFSGWVEAFPTKKETAKVVTKKLLEEIFPRFGMPQVLGTDNGPAFVSKVS 1524

Query: 68   EDLYNQFKIKHSNSTPYHPKMNGVVEAENKNIKRIIEKMTIT--YKDWHEMLPFALHGYR 127
            + + +   +       Y P+ +G VE  N+ IK  + K+T+    +DW  +LP AL+  R
Sbjct: 1525 QTVADLLGVDWKLHCAYRPQSSGQVERMNRTIKETLTKLTLATGSRDWVLLLPLALYRAR 1584

Query: 128  TSVRTSTGATPFSLVYG 143
             +     G TP+ ++YG
Sbjct: 1585 NTPGPH-GLTPYEILYG 1600

[Close]

BLAST of Chy6G115550 vs. ExPASy Swiss-Prot
Match: P03355 (Gag-Pol polyprotein OS=Moloney murine leukemia virus (isolate Shinnick) OX=928306 GN=gag-pol PE=1 SV=5)

HSP 1 Score: 79.7 bits (195), Expect = 4.0e-14
Identity = 44/137 (32.12%), Postives = 76/137 (55.47%), Query Frame = 0


Query: 8    GHRFILVAIDYFTKWVEATSYKSVTKQAVVKFIQKDIICRYGLPERIITDNAKNLNNKLM 67
            G++++LV ID F+ W+EA   K  T + V K + ++I  R+G+P+ + TDN     +K+ 
Sbjct: 1465 GYKYLLVFIDTFSGWIEAFPTKKETAKVVTKKLLEEIFPRFGMPQVLGTDNGPAFVSKVS 1524

Query: 68   EDLYNQFKIKHSNSTPYHPKMNGVVEAENKNIKRIIEKMTIT--YKDWHEMLPFALHGYR 127
            + + +   I       Y P+ +G VE  N+ IK  + K+T+    +DW  +LP AL+  R
Sbjct: 1525 QTVADLLGIDWKLHCAYRPQSSGQVERMNRTIKETLTKLTLATGSRDWVLLLPLALYRAR 1584

Query: 128  TSVRTSTGATPFSLVYG 143
             +     G TP+ ++YG
Sbjct: 1585 NTPGPH-GLTPYEILYG 1600

[Close]

BLAST of Chy6G115550 vs. ExPASy Swiss-Prot
Match: P08361 (Gag-Pol polyprotein OS=Cas-Br-E murine leukemia virus OX=11792 GN=gag-pol PE=3 SV=2)

HSP 1 Score: 79.3 bits (194), Expect = 5.2e-14
Identity = 43/137 (31.39%), Postives = 76/137 (55.47%), Query Frame = 0


Query: 8    GHRFILVAIDYFTKWVEATSYKSVTKQAVVKFIQKDIICRYGLPERIITDNAKNLNNKLM 67
            G++++LV +D F+ W+EA   K  T + V K + ++I  R+G+P+ + TDN     +K+ 
Sbjct: 1463 GYKYLLVFVDTFSGWIEAFPTKKETAKVVTKKLLEEIFPRFGMPQVLGTDNGPAFVSKVS 1522

Query: 68   EDLYNQFKIKHSNSTPYHPKMNGVVEAENKNIKRIIEKMTIT--YKDWHEMLPFALHGYR 127
            + + +   I       Y P+ +G VE  N+ IK  + K+T+    +DW  +LP AL+  R
Sbjct: 1523 QTVADLLGIDWKLHCAYRPQSSGQVERMNRTIKETLTKLTLATGSRDWVLLLPLALYRAR 1582

Query: 128  TSVRTSTGATPFSLVYG 143
             +     G TP+ ++YG
Sbjct: 1583 NTPGPH-GLTPYEILYG 1598

[Close]

BLAST of Chy6G115550 vs. ExPASy Swiss-Prot
Match: P26810 (Gag-Pol polyprotein OS=Friend murine leukemia virus (isolate 57) OX=11796 GN=pol PE=3 SV=2)

HSP 1 Score: 79.3 bits (194), Expect = 5.2e-14
Identity = 43/137 (31.39%), Postives = 76/137 (55.47%), Query Frame = 0


Query: 8    GHRFILVAIDYFTKWVEATSYKSVTKQAVVKFIQKDIICRYGLPERIITDNAKNLNNKLM 67
            G++++LV +D F+ WVEA   K  T + V K + ++I  R+G+P+ + TDN     +K+ 
Sbjct: 1466 GYKYLLVFVDTFSGWVEAFPTKKETAKVVTKKLLEEIFPRFGMPQVLGTDNGPAFVSKVS 1525

Query: 68   EDLYNQFKIKHSNSTPYHPKMNGVVEAENKNIKRIIEKMTIT--YKDWHEMLPFALHGYR 127
            + + +   +       Y P+ +G VE  N+ IK  + K+T+    +DW  +LP AL+  R
Sbjct: 1526 QTVADLLGVDWKLHCAYRPQSSGQVERMNRTIKETLTKLTLATGSRDWVLLLPLALYRAR 1585

Query: 128  TSVRTSTGATPFSLVYG 143
             +     G TP+ ++YG
Sbjct: 1586 NTPGPH-GLTPYEILYG 1601

[Close]

BLAST of Chy6G115550 vs. ExPASy TrEMBL
Match: A0A5A7VAF2 (RNA-directed DNA polymerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1023G00050 PE=4 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 4.8e-79
Identity = 144/173 (83.24%), Postives = 161/173 (93.06%), Query Frame = 0


Query: 1   MEPKASNGHRFILVAIDYFTKWVEATSYKSVTKQAVVKFIQKDIICRYGLPERIITDNAK 60
           +EPKASNGHRFILVAIDYFTKWVEA SYKSVTKQAVVKFI+KDIICRYGLPERIITDN K
Sbjct: 129 IEPKASNGHRFILVAIDYFTKWVEAASYKSVTKQAVVKFIRKDIICRYGLPERIITDNGK 188

Query: 61  NLNNKLMEDLYNQFKIKHSNSTPYHPKMNGVVEAENKNIKRIIEKMTITYKDWHEMLPFA 120
           NLNNKLME+L NQFK+KHSNSTPY PKMNG VE  NKNIK+I+EKM++TY+DWHEMLPFA
Sbjct: 189 NLNNKLMEELCNQFKVKHSNSTPYRPKMNGAVETANKNIKKILEKMSVTYRDWHEMLPFA 248

Query: 121 LHGYRTSVRTSTGATPFSLVYGVEAVLPIEVEVPSLKVIKEAELEEAEWTRAR 174
           L+GYR SVRTSTG+TPFSLVYG EAVLP+EVEVPSL+VI+E EL+EA+W + R
Sbjct: 249 LNGYRISVRTSTGSTPFSLVYGFEAVLPVEVEVPSLRVIQELELDEAKWAQVR 301

[Close]

BLAST of Chy6G115550 vs. ExPASy TrEMBL
Match: A0A6J1D7C7 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111018303 PE=4 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 2.6e-77
Identity = 142/173 (82.08%), Postives = 158/173 (91.33%), Query Frame = 0


Query: 1    MEPKASNGHRFILVAIDYFTKWVEATSYKSVTKQAVVKFIQKDIICRYGLPERIITDNAK 60
            +EPKASNGHRFILVAIDYFTKWVEA SY+ VTK  VVKFI+K+IICRYGLPE II+DNA+
Sbjct: 1394 IEPKASNGHRFILVAIDYFTKWVEAASYRDVTKGVVVKFIKKEIICRYGLPETIISDNAR 1453

Query: 61   NLNNKLMEDLYNQFKIKHSNSTPYHPKMNGVVEAENKNIKRIIEKMTITYKDWHEMLPFA 120
            NLNNKLM +L  QFKIKH NSTPY PKMNG VEA NKNIKRI+EKMT+TY+DWHEMLPFA
Sbjct: 1454 NLNNKLMSELCEQFKIKHLNSTPYRPKMNGAVEAANKNIKRIVEKMTVTYRDWHEMLPFA 1513

Query: 121  LHGYRTSVRTSTGATPFSLVYGVEAVLPIEVEVPSLKVIKEAELEEAEWTRAR 174
            LHGYRTSVRTSTGATPFSLVYG+EAVLPIEVE+PSL+VI EA+L+EAEW + R
Sbjct: 1514 LHGYRTSVRTSTGATPFSLVYGMEAVLPIEVEIPSLRVIMEAKLQEAEWVQRR 1566

[Close]

BLAST of Chy6G115550 vs. ExPASy TrEMBL
Match: A0A6J1D099 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111016200 PE=4 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 5.9e-77
Identity = 140/173 (80.92%), Postives = 158/173 (91.33%), Query Frame = 0


Query: 1    MEPKASNGHRFILVAIDYFTKWVEATSYKSVTKQAVVKFIQKDIICRYGLPERIITDNAK 60
            +EPKASNGH+FILVAIDYFTKWVEA SY+ VTK  VVKFI+K+IICRYGLP+ II+DNA+
Sbjct: 1990 IEPKASNGHQFILVAIDYFTKWVEAASYRDVTKGVVVKFIKKEIICRYGLPKTIISDNAR 2049

Query: 61   NLNNKLMEDLYNQFKIKHSNSTPYHPKMNGVVEAENKNIKRIIEKMTITYKDWHEMLPFA 120
            NLNNKLM +LY QFKIKH NSTPY PKMNG VEA NKNIKRI+EKMT+TY+DWHEMLPFA
Sbjct: 2050 NLNNKLMSELYEQFKIKHLNSTPYRPKMNGAVEAANKNIKRIVEKMTVTYRDWHEMLPFA 2109

Query: 121  LHGYRTSVRTSTGATPFSLVYGVEAVLPIEVEVPSLKVIKEAELEEAEWTRAR 174
            LHGYRTSVRTSTGATPFSLVYG+E VLPIEVE+PSL+VI EA+L+EAEW + R
Sbjct: 2110 LHGYRTSVRTSTGATPFSLVYGMEVVLPIEVEIPSLRVIMEAKLQEAEWVQRR 2162

[Close]

BLAST of Chy6G115550 vs. ExPASy TrEMBL
Match: A0A5D3DAC8 (Putative RNA-directed DNA polymerase (Reverse transcriptase), Ribonuclease H OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00320 PE=4 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 6.5e-76
Identity = 140/189 (74.07%), Postives = 162/189 (85.71%), Query Frame = 0


Query: 1   MEPKASNGHRFILVAIDYFTKWVEATSYKSVTKQAVVKFIQKDIICRYGLPERIITDNAK 60
           ++PKASNGHRFILVAIDYFTKW+EA SY +VT++ V+KFI+K++ICRYGLPE IITDNAK
Sbjct: 7   IDPKASNGHRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGIITDNAK 66

Query: 61  NLNNKLMEDLYNQFKIKHSNSTPYHPKMNGVVEAENKNIKRIIEKMTITYKDWHEMLPFA 120
           NLNNK+M++L  QFKI H NSTPY PKMN  +EA NKNIKRIIEKMTITYKDWHEMLPFA
Sbjct: 67  NLNNKMMDELCEQFKINHRNSTPYRPKMNEAIEAANKNIKRIIEKMTITYKDWHEMLPFA 126

Query: 121 LHGYRTSVRTSTGATPFSLVYGVEAVLPIEVEVPSLKVIKEAELEEAEWTRARIVSMRIK 180
           LHGYRTSVRTSTGATPFSLVYG+EAVLP+EVE+PSL+V+ EA+L+EAEW R R   +   
Sbjct: 127 LHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRYEQLNFV 186

Query: 181 FVTNLAETN 190
               LA  N
Sbjct: 187 EEKQLAALN 195

[Close]

BLAST of Chy6G115550 vs. ExPASy TrEMBL
Match: A0A5A7T570 (Pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36G003710 PE=4 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 6.5e-76
Identity = 140/189 (74.07%), Postives = 162/189 (85.71%), Query Frame = 0


Query: 1   MEPKASNGHRFILVAIDYFTKWVEATSYKSVTKQAVVKFIQKDIICRYGLPERIITDNAK 60
           ++PKASNGHRFILVAIDYFTKW+EA SY +VT++ V+KFI+K++ICRYGLPE IITDNAK
Sbjct: 7   IDPKASNGHRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGIITDNAK 66

Query: 61  NLNNKLMEDLYNQFKIKHSNSTPYHPKMNGVVEAENKNIKRIIEKMTITYKDWHEMLPFA 120
           NLNNK+M++L  QFKI H NSTPY PKMN  +EA NKNIKRIIEKMTITYKDWHEMLPFA
Sbjct: 67  NLNNKMMDELCEQFKINHRNSTPYRPKMNEAIEAANKNIKRIIEKMTITYKDWHEMLPFA 126

Query: 121 LHGYRTSVRTSTGATPFSLVYGVEAVLPIEVEVPSLKVIKEAELEEAEWTRARIVSMRIK 180
           LHGYRTSVRTSTGATPFSLVYG+EAVLP+EVE+PSL+V+ EA+L+EAEW R R   +   
Sbjct: 127 LHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRYEQLNFV 186

Query: 181 FVTNLAETN 190
               LA  N
Sbjct: 187 EEKQLAALN 195

[Close]

BLAST of Chy6G115550 vs. NCBI nr
Match: KAA0065292.1 (RNA-directed DNA polymerase [Cucumis melo var. makuwa])

HSP 1 Score: 303 bits (776), Expect = 2.31e-99
Identity = 147/193 (76.17%), Postives = 169/193 (87.56%), Query Frame = 0


Query: 1   MEPKASNGHRFILVAIDYFTKWVEATSYKSVTKQAVVKFIQKDIICRYGLPERIITDNAK 60
           +EPKASNGHRFILVAIDYFTKWVEA SYKSVTKQAVVKFI+KDIICRYGLPERIITDN K
Sbjct: 129 IEPKASNGHRFILVAIDYFTKWVEAASYKSVTKQAVVKFIRKDIICRYGLPERIITDNGK 188

Query: 61  NLNNKLMEDLYNQFKIKHSNSTPYHPKMNGVVEAENKNIKRIIEKMTITYKDWHEMLPFA 120
           NLNNKLME+L NQFK+KHSNSTPY PKMNG VE  NKNIK+I+EKM++TY+DWHEMLPFA
Sbjct: 189 NLNNKLMEELCNQFKVKHSNSTPYRPKMNGAVETANKNIKKILEKMSVTYRDWHEMLPFA 248

Query: 121 LHGYRTSVRTSTGATPFSLVYGVEAVLPIEVEVPSLKVIKEAELEEAEWTRARIVSMRIK 180
           L+GYR SVRTSTG+TPFSLVYG EAVLP+EVEVPSL+VI+E EL+EA+W + R    ++ 
Sbjct: 249 LNGYRISVRTSTGSTPFSLVYGFEAVLPVEVEVPSLRVIQELELDEAKWAQVRYE--QLN 308

Query: 181 FVTNLAETNVTQG 193
           F+     T + +G
Sbjct: 309 FIEERRLTALCRG 319

[Close]

BLAST of Chy6G115550 vs. NCBI nr
Match: KAA0038143.1 (Pol polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 293 bits (749), Expect = 2.63e-97
Identity = 141/192 (73.44%), Postives = 163/192 (84.90%), Query Frame = 0


Query: 1   MEPKASNGHRFILVAIDYFTKWVEATSYKSVTKQAVVKFIQKDIICRYGLPERIITDNAK 60
           ++PKASNGHRFILVAIDYFTKW+EA SY +VT++ V+KFI+K++ICRYGLPE IITDNAK
Sbjct: 7   IDPKASNGHRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGIITDNAK 66

Query: 61  NLNNKLMEDLYNQFKIKHSNSTPYHPKMNGVVEAENKNIKRIIEKMTITYKDWHEMLPFA 120
           NLNNK+M++L  QFKI H NSTPY PKMN  +EA NKNIKRIIEKMTITYKDWHEMLPFA
Sbjct: 67  NLNNKMMDELCEQFKINHRNSTPYRPKMNEAIEAANKNIKRIIEKMTITYKDWHEMLPFA 126

Query: 121 LHGYRTSVRTSTGATPFSLVYGVEAVLPIEVEVPSLKVIKEAELEEAEWTRARIVSMRIK 180
           LHGYRTSVRTSTGATPFSLVYG+EAVLP+EVE+PSL+V+ EA+L+EAEW R R   +   
Sbjct: 127 LHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRYEQLNFV 186

Query: 181 FVTNLAETNVTQ 192
               LA  N  Q
Sbjct: 187 EEKQLAALNHGQ 198

[Close]

BLAST of Chy6G115550 vs. NCBI nr
Match: TYK20464.1 (putative RNA-directed DNA polymerase (Reverse transcriptase), Ribonuclease H [Cucumis melo var. makuwa])

HSP 1 Score: 293 bits (749), Expect = 3.02e-96
Identity = 141/192 (73.44%), Postives = 163/192 (84.90%), Query Frame = 0


Query: 1   MEPKASNGHRFILVAIDYFTKWVEATSYKSVTKQAVVKFIQKDIICRYGLPERIITDNAK 60
           ++PKASNGHRFILVAIDYFTKW+EA SY +VT++ V+KFI+K++ICRYGLPE IITDNAK
Sbjct: 7   IDPKASNGHRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGIITDNAK 66

Query: 61  NLNNKLMEDLYNQFKIKHSNSTPYHPKMNGVVEAENKNIKRIIEKMTITYKDWHEMLPFA 120
           NLNNK+M++L  QFKI H NSTPY PKMN  +EA NKNIKRIIEKMTITYKDWHEMLPFA
Sbjct: 67  NLNNKMMDELCEQFKINHRNSTPYRPKMNEAIEAANKNIKRIIEKMTITYKDWHEMLPFA 126

Query: 121 LHGYRTSVRTSTGATPFSLVYGVEAVLPIEVEVPSLKVIKEAELEEAEWTRARIVSMRIK 180
           LHGYRTSVRTSTGATPFSLVYG+EAVLP+EVE+PSL+V+ EA+L+EAEW R R   +   
Sbjct: 127 LHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRYEQLNFV 186

Query: 181 FVTNLAETNVTQ 192
               LA  N  Q
Sbjct: 187 EEKQLAALNHGQ 198

[Close]

BLAST of Chy6G115550 vs. NCBI nr
Match: KAA0036953.1 (Pol polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 284 bits (726), Expect = 1.57e-94
Identity = 139/192 (72.40%), Postives = 159/192 (82.81%), Query Frame = 0


Query: 1   MEPKASNGHRFILVAIDYFTKWVEATSYKSVTKQAVVKFIQKDIICRYGLPERIITDNAK 60
           ++PKASNGHRFILVAIDYFTKW+EA SY +VT+  V+KFI+K++ICRYGLPE IITDNAK
Sbjct: 7   IDPKASNGHRFILVAIDYFTKWIEAASYCNVTRGVVLKFIKKELICRYGLPEGIITDNAK 66

Query: 61  NLNNKLMEDLYNQFKIKHSNSTPYHPKMNGVVEAENKNIKRIIEKMTITYKDWHEMLPFA 120
           NLNNK+M++L  QFKI H NSTPY P MNG VEA NKNIKRIIEKMTITYKDWHEML FA
Sbjct: 67  NLNNKMMDELCEQFKINHRNSTPYRPNMNGAVEAANKNIKRIIEKMTITYKDWHEMLLFA 126

Query: 121 LHGYRTSVRTSTGATPFSLVYGVEAVLPIEVEVPSLKVIKEAELEEAEWTRARIVSMRIK 180
           LHGYRTSVRTSTGAT FSLVYG+E VLP+EVE+PSL+V+ EA+L+EAEW R R   +   
Sbjct: 127 LHGYRTSVRTSTGATLFSLVYGMEVVLPLEVEIPSLRVLMEAKLDEAEWIRGRYEQLNFV 186

Query: 181 FVTNLAETNVTQ 192
               LA  N  Q
Sbjct: 187 EEKRLAALNHGQ 198

[Close]

BLAST of Chy6G115550 vs. NCBI nr
Match: KAA0045604.1 (Retrotransposable element Tf2 [Cucumis melo var. makuwa] >TYK02653.1 Retrotransposable element Tf2 [Cucumis melo var. makuwa])

HSP 1 Score: 282 bits (721), Expect = 1.85e-93
Identity = 139/168 (82.74%), Postives = 154/168 (91.67%), Query Frame = 0


Query: 1   MEPKASNGHRFILVAIDYFTKWVEATSYKSVTKQAVVKFIQKDIICRYGLPERIITDNAK 60
           +EPKASNGHRFILVAIDYFTKWVE+ SY   TKQAVVKFI+KDIIC+YGLPERIITDN K
Sbjct: 66  IEPKASNGHRFILVAIDYFTKWVESASY---TKQAVVKFIRKDIICQYGLPERIITDNGK 125

Query: 61  NLNNKLMEDLYNQFKIKHSNSTPYHPKMNGVVEAENKNIKRIIEKMTITYKDWHEMLPFA 120
           NLNNKLME+L +QFK+KHSNSTPY PKMNG VEA NKNIK+I+EKM+ITY+DWHEMLPFA
Sbjct: 126 NLNNKLMEELCSQFKVKHSNSTPYRPKMNGAVEAANKNIKKILEKMSITYRDWHEMLPFA 185

Query: 121 LHGYRTSVRTSTGATPFSLVYGVEAVLPIEVEVPSLKVIKEAELEEAE 168
           LHGYR SVRTST ATPFSLVY  EAVLP+EVEVPSLKVI+E EL+EA+
Sbjct: 186 LHGYRISVRTSTRATPFSLVYCFEAVLPVEVEVPSLKVIQEVELDEAK 230

The following BLAST results are available for this feature:

BLAST of Chy6G115550 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P03360	5.6e-16	35.46	Gag-Pol polyprotein (Fragment) OS=Avian reticuloendotheliosis virus OX=11636 GN=...	[more]
P26809	4.0e-14	32.12	Gag-Pol polyprotein OS=Friend murine leukemia virus (isolate FB29) OX=11797 GN=p...	[more]
P03355	4.0e-14	32.12	Gag-Pol polyprotein OS=Moloney murine leukemia virus (isolate Shinnick) OX=92830...	[more]
P08361	5.2e-14	31.39	Gag-Pol polyprotein OS=Cas-Br-E murine leukemia virus OX=11792 GN=gag-pol PE=3 S...	[more]
P26810	5.2e-14	31.39	Gag-Pol polyprotein OS=Friend murine leukemia virus (isolate 57) OX=11796 GN=pol...	[more]

BLAST of Chy6G115550 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7VAF2	4.8e-79	83.24	RNA-directed DNA polymerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf...	[more]
A0A6J1D7C7	2.6e-77	82.08	Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111018303 PE=4 SV=1	[more]
A0A6J1D099	5.9e-77	80.92	Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111016200 PE=4 SV=1	[more]
A0A5D3DAC8	6.5e-76	74.07	Putative RNA-directed DNA polymerase (Reverse transcriptase), Ribonuclease H OS=...	[more]
A0A5A7T570	6.5e-76	74.07	Pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36G00371...	[more]

BLAST of Chy6G115550 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0065292.1	2.31e-99	76.17	RNA-directed DNA polymerase [Cucumis melo var. makuwa]	[more]
KAA0038143.1	2.63e-97	73.44	Pol polyprotein [Cucumis melo var. makuwa]	[more]
TYK20464.1	3.02e-96	73.44	putative RNA-directed DNA polymerase (Reverse transcriptase), Ribonuclease H [Cu...	[more]
KAA0036953.1	1.57e-94	72.40	Pol polyprotein [Cucumis melo var. makuwa]	[more]
KAA0045604.1	1.85e-93	82.74	Retrotransposable element Tf2 [Cucumis melo var. makuwa] >TYK02653.1 Retrotransp...	[more]

BLAST of Chy6G115550 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 1..180 e-value: 1.7E-48 score: 166.5
IPR001584	Integrase, catalytic core	PFAM	PF00665	rve	coord: 9..86 e-value: 6.7E-11 score: 42.4
IPR001584	Integrase, catalytic core	PROSITE	PS50994	INTEGRASE	coord: 1..144 score: 18.98317
None	No IPR available	PANTHER	PTHR24559	TRANSPOSON TY3-I GAG-POL POLYPROTEIN	coord: 24..171
None	No IPR available	PANTHER	PTHR24559:SF342	SUBFAMILY NOT NAMED	coord: 24..171
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 8..150

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy6G115550.1	Chy6G115550.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
biological_process	GO:0006278	RNA-dependent DNA biosynthetic process
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0030430	host cell cytoplasm
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0003964	RNA-directed DNA polymerase activity
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity