Chy6G115450 (gene) Cucumber (hystrix) v1

Overview


Name	Chy6G115450
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	aspartic proteinase PCS1
Location	chrH06: 7880673 .. 7881965 (-)
RNA-Seq Expression	Chy6G115450
Synteny	Chy6G115450

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTCTGATTCTTTTCTCTCTCTCCTTATTCACTCTCTCCTTCTCTCAATCCAATTCCCTCTCTCTCCCCTTCCCTCTTTCTCTCTCTGAAAAACCCTCCAATACTACCCCATTATACTCTTCCCAGCTTTACGCCAAGAGGCCATCGTCCTACGGCTCCTTCAAGCTTCCTTTCAAATACTCCTCCACCGCCCTCGTCGTCTCTCTACCGATCGGAACGCCGCCACAGCCCACTGACTTGGTTCTAGACACCGGCAGCCAACTATCTTGGATTCAATGTCATGACAAAAAGATTAAGAAAAGATTGCCCCCCTTGCCCAAGCCTAAAACCGCCTCCTTTGATCCTTCTCTCTCCTCTTCTTTTTCTCTCCTCCCTTGTAATCACCCCATCTGCAAACCCCGAATTCCAGATTTTACCCTTCCCACTTCTTGTGACCAAAATCGCCTCTGCCACTACTCCTACTTCTACGCTGACGGTACCTTGGCTGAGGGAAATCTCGTCAGAGAAAAATTTACCTTCTCTAATTCCCTTTCTACCCCTCCCGTCATCCTCGGTTGCGCTCAAGCCTCCACCGAAAACAGGGGTATTTTGGGAATGAACCATGGACGTTTGTCCTTTATCTCTCAAGCTAAAATCTCCAAATTCTCCTATTGCGTTCCCAGTCGAACCGGGTCTAATCCCACCGGGCTATTCTACCTTGGAGATAATCCCAATTCTTCCAAATTCAAATACGTCACCATGTTGACTTTTCCCGAAAGTCAAAGCTCCCCGAATCTCGACCCACTGGCTTACACCCTCCCAATGAAGGGAATAAAAATAGCCGGAAAACTTCTAAACATCTCCCCAGCCACTTTCAAACCGGACGCGGGTGGGTCGGGTCAAACCATGATTGACTCCGGTTCGGACCTGACTTATTTAGTGGATGAAGCGTATGAGAAGGTTAAAGAAGAGGTAGTGAGATTAGTGGGGGCGAAGATGAAGAAAGGCTACGTATACGCCGCCGTCGCCGACATGTGTTTCGACGCCGGCGTGACGGCGGAGGTGGGCCGCAGGATTGGCGGCATGTCGTTTGAGTTTGAGAATGGGGTGGAGATATTCGTGGGGAGAGGAGAAGAGGTTTTGACGGAAGTGGAAAAAGGGGTGAAGTGTGTGGGGATCGGACGGTCAGAAAGGCTTGGGATTGGGAGTAATATAATCGGTACCGTTCATCAACAGAATATGTGGGTGGAGTATGATTTGGCCAATAAGAGAGTAGGGTTTGGTGGAGCTGAGTGCAGCAGATTGAAGTGA

mRNA sequence

ATGCTTCTGATTCTTTTCTCTCTCTCCTTATTCACTCTCTCCTTCTCTCAATCCAATTCCCTCTCTCTCCCCTTCCCTCTTTCTCTCTCTGAAAAACCCTCCAATACTACCCCATTATACTCTTCCCAGCTTTACGCCAAGAGGCCATCGTCCTACGGCTCCTTCAAGCTTCCTTTCAAATACTCCTCCACCGCCCTCGTCGTCTCTCTACCGATCGGAACGCCGCCACAGCCCACTGACTTGGTTCTAGACACCGGCAGCCAACTATCTTGGATTCAATGTCATGACAAAAAGATTAAGAAAAGATTGCCCCCCTTGCCCAAGCCTAAAACCGCCTCCTTTGATCCTTCTCTCTCCTCTTCTTTTTCTCTCCTCCCTTGTAATCACCCCATCTGCAAACCCCGAATTCCAGATTTTACCCTTCCCACTTCTTGTGACCAAAATCGCCTCTGCCACTACTCCTACTTCTACGCTGACGGTACCTTGGCTGAGGGAAATCTCGTCAGAGAAAAATTTACCTTCTCTAATTCCCTTTCTACCCCTCCCGTCATCCTCGGTTGCGCTCAAGCCTCCACCGAAAACAGGGGTATTTTGGGAATGAACCATGGACGTTTGTCCTTTATCTCTCAAGCTAAAATCTCCAAATTCTCCTATTGCGTTCCCAGTCGAACCGGGTCTAATCCCACCGGGCTATTCTACCTTGGAGATAATCCCAATTCTTCCAAATTCAAATACGTCACCATGTTGACTTTTCCCGAAAGTCAAAGCTCCCCGAATCTCGACCCACTGGCTTACACCCTCCCAATGAAGGGAATAAAAATAGCCGGAAAACTTCTAAACATCTCCCCAGCCACTTTCAAACCGGACGCGGGTGGGTCGGGTCAAACCATGATTGACTCCGGTTCGGACCTGACTTATTTAGTGGATGAAGCGTATGAGAAGGTTAAAGAAGAGGTAGTGAGATTAGTGGGGGCGAAGATGAAGAAAGGCTACGTATACGCCGCCGTCGCCGACATGTGTTTCGACGCCGGCGTGACGGCGGAGGTGGGCCGCAGGATTGGCGGCATGTCGTTTGAGTTTGAGAATGGGGTGGAGATATTCGTGGGGAGAGGAGAAGAGGTTTTGACGGAAGTGGAAAAAGGGGTGAAGTGTGTGGGGATCGGACGGTCAGAAAGGCTTGGGATTGGGAGTAATATAATCGGTACCGTTCATCAACAGAATATGTGGGTGGAGTATGATTTGGCCAATAAGAGAGTAGGGTTTGGTGGAGCTGAGTGCAGCAGATTGAAGTGA

Coding sequence (CDS)

ATGCTTCTGATTCTTTTCTCTCTCTCCTTATTCACTCTCTCCTTCTCTCAATCCAATTCCCTCTCTCTCCCCTTCCCTCTTTCTCTCTCTGAAAAACCCTCCAATACTACCCCATTATACTCTTCCCAGCTTTACGCCAAGAGGCCATCGTCCTACGGCTCCTTCAAGCTTCCTTTCAAATACTCCTCCACCGCCCTCGTCGTCTCTCTACCGATCGGAACGCCGCCACAGCCCACTGACTTGGTTCTAGACACCGGCAGCCAACTATCTTGGATTCAATGTCATGACAAAAAGATTAAGAAAAGATTGCCCCCCTTGCCCAAGCCTAAAACCGCCTCCTTTGATCCTTCTCTCTCCTCTTCTTTTTCTCTCCTCCCTTGTAATCACCCCATCTGCAAACCCCGAATTCCAGATTTTACCCTTCCCACTTCTTGTGACCAAAATCGCCTCTGCCACTACTCCTACTTCTACGCTGACGGTACCTTGGCTGAGGGAAATCTCGTCAGAGAAAAATTTACCTTCTCTAATTCCCTTTCTACCCCTCCCGTCATCCTCGGTTGCGCTCAAGCCTCCACCGAAAACAGGGGTATTTTGGGAATGAACCATGGACGTTTGTCCTTTATCTCTCAAGCTAAAATCTCCAAATTCTCCTATTGCGTTCCCAGTCGAACCGGGTCTAATCCCACCGGGCTATTCTACCTTGGAGATAATCCCAATTCTTCCAAATTCAAATACGTCACCATGTTGACTTTTCCCGAAAGTCAAAGCTCCCCGAATCTCGACCCACTGGCTTACACCCTCCCAATGAAGGGAATAAAAATAGCCGGAAAACTTCTAAACATCTCCCCAGCCACTTTCAAACCGGACGCGGGTGGGTCGGGTCAAACCATGATTGACTCCGGTTCGGACCTGACTTATTTAGTGGATGAAGCGTATGAGAAGGTTAAAGAAGAGGTAGTGAGATTAGTGGGGGCGAAGATGAAGAAAGGCTACGTATACGCCGCCGTCGCCGACATGTGTTTCGACGCCGGCGTGACGGCGGAGGTGGGCCGCAGGATTGGCGGCATGTCGTTTGAGTTTGAGAATGGGGTGGAGATATTCGTGGGGAGAGGAGAAGAGGTTTTGACGGAAGTGGAAAAAGGGGTGAAGTGTGTGGGGATCGGACGGTCAGAAAGGCTTGGGATTGGGAGTAATATAATCGGTACCGTTCATCAACAGAATATGTGGGTGGAGTATGATTTGGCCAATAAGAGAGTAGGGTTTGGTGGAGCTGAGTGCAGCAGATTGAAGTGA

Protein sequence

MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTTPLYSSQLYAKRPSSYGSFKLPFKYSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTASFDPSLSSSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLSTPPVILGCAQASTENRGILGMNHGRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGDNPNSSKFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKLLNISPATFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDAGVTAEVGRRIGGMSFEFENGVEIFVGRGEEVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAECSRLK*

Homology

[Close]

BLAST of Chy6G115450 vs. ExPASy Swiss-Prot
Match: Q9LZL3 (Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 2.3e-62
Identity = 162/451 (35.92%), Postives = 236/451 (52.33%), Query Frame = 0


Query: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTTPLYSSQLYAKRPSSYGSFKLPFK 60
           +LL+L   +   +S S S+S S  F  S S   S+ T +   +          + KL F 
Sbjct: 10  LLLVLSVRTYKCVSSSSSSSSSFSFS-SFSSSSSSQTLVLPLKTRITPTDHRPTDKLHFH 69

Query: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTASFDPSLSS 120
           ++ T L V+L +GTPPQ   +V+DTGS+LSW++C+           P P   +FDP+ SS
Sbjct: 70  HNVT-LTVTLTVGTPPQNISMVIDTGSELSWLRCNRSS-------NPNP-VNNFDPTRSS 129

Query: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLST 180
           S+S +PC+ P C+ R  DF +P SCD ++LCH +  YAD + +EGNL  E F F NS + 
Sbjct: 130 SYSPIPCSSPTCRTRTRDFLIPASCDSDKLCHATLSYADASSSEGNLAAEIFHFGNSTND 189

Query: 181 PPVILGC--------AQASTENRGILGMNHGRLSFISQAKISKFSYCVPSRTGSNPTGLF 240
             +I GC         +  T+  G+LGMN G LSFISQ    KFSYC+ S T   P G  
Sbjct: 190 SNLIFGCMGSVSGSDPEEDTKTTGLLGMNRGSLSFISQMGFPKFSYCI-SGTDDFP-GFL 249

Query: 241 YLGDNPNSSKFKYVTMLTFPE----SQSSPNLDPLAYTLPMKGIKIAGKLLNISPATFKP 300
            LGD    S F ++T L +      S   P  D +AYT+ + GIK+ GKLL I  +   P
Sbjct: 250 LLGD----SNFTWLTPLNYTPLIRISTPLPYFDRVAYTVQLTGIKVNGKLLPIPKSVLVP 309

Query: 301 DAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVR----LVGAKMKKGYVYAAVADMCF--- 360
           D  G+GQTM+DSG+  T+L+   Y  ++   +     ++       +V+    D+C+   
Sbjct: 310 DHTGAGQTMVDSGTQFTFLLGPVYTALRSHFLNRTNGILTVYEDPDFVFQGTMDLCYRIS 369

Query: 361 DAGVTAEVGRRIGGMSFEFENGVEIFVGRGEEVLTEV------EKGVKCVGIGRSERLGI 420
              + + +  R+  +S  FE G EI V  G+ +L  V         V C   G S+ +G+
Sbjct: 370 PVRIRSGILHRLPTVSLVFE-GAEIAVS-GQPLLYRVPHLTVGNDSVYCFTFGNSDLMGM 429

Query: 421 GSNIIGTVHQQNMWVEYDLANKRVGFGGAEC 427
            + +IG  HQQNMW+E+DL   R+G    EC
Sbjct: 430 EAYVIGHHHQQNMWIEFDLQRSRIGLAPVEC 442

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BLAST of Chy6G115450 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 7.2e-40
Identity = 115/367 (31.34%), Postives = 172/367 (46.87%), Query Frame = 0


Query: 67  VVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTASFDPSLSSSFSLLP 126
           +++L IGTP QP   ++DTGS L W QC      +         T  F+P  SSSFS LP
Sbjct: 96  LMNLSIGTPAQPFSAIMDTGSDLIWTQC------QPCTQCFNQSTPIFNPQGSSSFSTLP 155

Query: 127 CNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLSTPPVILG 186
           C+  +C+       L +    N  C Y+Y Y DG+  +G++  E  TF  S+S P +  G
Sbjct: 156 CSSQLCQ------ALSSPTCSNNFCQYTYGYGDGSETQGSMGTETLTF-GSVSIPNITFG 215

Query: 187 CAQ-----ASTENRGILGMNHGRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGDNPNSS 246
           C +           G++GM  G LS  SQ  ++KFSYC+     S P+ L  LG   NS 
Sbjct: 216 CGENNQGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIGSSTPSNLL-LGSLANSV 275

Query: 247 KFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKLLNISPATFKPDA-GGSGQTMIDS 306
                       SQ      P  Y + + G+ +    L I P+ F  ++  G+G  +IDS
Sbjct: 276 TAGSPNTTLIQSSQI-----PTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDS 335

Query: 307 GSDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDAGVTAEVGRRIGGMSFEF 366
           G+ LTY V+ AY+ V++E +  +   +  G   ++  D+CF    +     +I      F
Sbjct: 336 GTTLTYFVNNAYQSVRQEFISQINLPVVNG--SSSGFDLCFQT-PSDPSNLQIPTFVMHF 395

Query: 367 ENG-VEIFVGRGEEVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRV 426
           + G +E+     E        G+ C+ +G S +   G +I G + QQNM V YD  N  V
Sbjct: 396 DGGDLEL---PSENYFISPSNGLICLAMGSSSQ---GMSIFGNIQQQNMLVVYDTGNSVV 434

[Close]

BLAST of Chy6G115450 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 5.1e-38
Identity = 120/374 (32.09%), Postives = 174/374 (46.52%), Query Frame = 0


Query: 67  VVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTASFDPSLSSSFSLLP 126
           ++++ IGTP      ++DTGS L W QC         P      T  F+P  SSSFS LP
Sbjct: 97  LMNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQCFSQP------TPIFNPQDSSSFSTLP 156

Query: 127 CNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLSTPPVILG 186
           C    C+       LP+    N  C Y+Y Y DG+  +G +  E FTF  S S P +  G
Sbjct: 157 CESQYCQ------DLPSETCNNNECQYTYGYGDGSTTQGYMATETFTFETS-SVPNIAFG 216

Query: 187 CAQ-----ASTENRGILGMNHGRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGDNPNSS 246
           C +           G++GM  G LS  SQ  + +FSYC+ S   S+P+ L  LG   +  
Sbjct: 217 CGEDNQGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYGSSSPSTL-ALGSAASG- 276

Query: 247 KFKYVTMLTFPESQSS-----PNLDPLAYTLPMKGIKIAGKLLNISPATFKPDAGGSGQT 306
                     PE   S      +L+P  Y + ++GI + G  L I  +TF+    G+G  
Sbjct: 277 ---------VPEGSPSTTLIHSSLNPTYYYITLQGITVGGDNLGIPSSTFQLQDDGTGGM 336

Query: 307 MIDSGSDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDA---GVTAEVGRRI 366
           +IDSG+ LTYL  +AY  V +     +   +      ++    CF     G T +V    
Sbjct: 337 IIDSGTTLTYLPQDAYNAVAQAFTDQI--NLPTVDESSSGLSTCFQQPSDGSTVQVPE-- 396

Query: 367 GGMSFEFENGVEIFVGRGEE-VLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEY 426
             +S +F+ GV   +  GE+ +L    +GV C+ +G S +LGI  +I G + QQ   V Y
Sbjct: 397 --ISMQFDGGV---LNLGEQNILISPAEGVICLAMGSSSQLGI--SIFGNIQQQETQVLY 435

[Close]

BLAST of Chy6G115450 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 7.2e-32
Identity = 100/363 (27.55%), Postives = 163/363 (44.90%), Query Frame = 0


Query: 72  IGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTASFDPSLSSSFSLLPCNHPI 131
           +GTP +   LVLDTGS ++WIQC      +      +     F+P+ SS++  L C+ P 
Sbjct: 168 VGTPAKEMYLVLDTGSDVNWIQC------EPCADCYQQSDPVFNPTSSSTYKSLTCSAPQ 227

Query: 132 CKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLSTPPVILGCAQAS 191
           C        L TS  ++  C Y   Y DG+   G L  +  TF NS     V LGC   +
Sbjct: 228 CS------LLETSACRSNKCLYQVSYGDGSFTVGELATDTVTFGNSGKINNVALGCGHDN 287

Query: 192 ----TENRGILGMNHGRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGDNPNSSKFKYVT 251
               T   G+LG+  G LS  +Q K + FSYC+  R            D+  SS   + +
Sbjct: 288 EGLFTGAAGLLGLGGGVLSITNQMKATSFSYCLVDR------------DSGKSSSLDFNS 347

Query: 252 MLTFPESQSSPNLD----PLAYTLPMKGIKIAGKLLNISPATFKPDAGGSGQTMIDSGSD 311
           +       ++P L        Y + + G  + G+ + +  A F  DA GSG  ++D G+ 
Sbjct: 348 VQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGTA 407

Query: 312 LTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDAGVTAEVGRRIGGMSFEFENG 371
           +T L  +AY  +++  ++L    +KKG    ++ D C+D    + V  ++  ++F F  G
Sbjct: 408 VTRLQTQAYNSLRDAFLKLT-VNLKKGSSSISLFDTCYDFSSLSTV--KVPTVAFHFTGG 467

Query: 372 VEIFVGRGEEVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRVGFGG 427
             + +     ++   + G  C     +       +IIG V QQ   + YDL+   +G  G
Sbjct: 468 KSLDLPAKNYLIPVDDSGTFCFAFAPTSS---SLSIIGNVQQQGTRITYDLSKNVIGLSG 500

[Close]

BLAST of Chy6G115450 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 3.9e-30
Identity = 113/393 (28.75%), Postives = 181/393 (46.06%), Query Frame = 0


Query: 45  YAKRPSSYGSFKLP-FKYSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHD-KKIKKR 104
           +A RP  + S  +      S      L +GTP +   +VLDTGS + W+QC   ++   +
Sbjct: 120 HAPRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQ 179

Query: 105 LPPLPKPKTASFDPSLSSSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTL 164
             P+       FDP  S +++ +PC+ P C+ R+      T   + + C Y   Y DG+ 
Sbjct: 180 SDPI-------FDPRKSKTYATIPCSSPHCR-RLDSAGCNT---RRKTCLYQVSYGDGSF 239

Query: 165 AEGNLVREKFTFSNSLSTPPVILGCAQAS----TENRGILGMNHGRLSFISQAK---ISK 224
             G+   E  TF  +     V LGC   +        G+LG+  G+LSF  Q       K
Sbjct: 240 TVGDFSTETLTFRRN-RVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQK 299

Query: 225 FSYCVPSRTGSNPTGLFYLGDNPNSSKFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIA 284
           FSYC+  R+ S+       G+   S   ++  +L      S+P LD   Y + + GI + 
Sbjct: 300 FSYCLVDRSASSKPSSVVFGNAAVSRIARFTPLL------SNPKLDTF-YYVGLLGISVG 359

Query: 285 G-KLLNISPATFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYA 344
           G ++  ++ + FK D  G+G  +IDSG+ +T L+  AY  +++     VGAK  K     
Sbjct: 360 GTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAF--RVGAKTLKRAPDF 419

Query: 345 AVADMCFDAGVTAEVGRRIGGMSFEFENGVEIFVGRGEEVLTEVEKGVKCVGIGRSERLG 404
           ++ D CFD     EV  ++  +   F  G ++ +     ++     G  C     +  +G
Sbjct: 420 SLFDTCFDLSNMNEV--KVPTVVLHF-RGADVSLPATNYLIPVDTNGKFCFAFAGT--MG 479

Query: 405 IGSNIIGTVHQQNMWVEYDLANKRVGFGGAECS 428
            G +IIG + QQ   V YDLA+ RVGF    C+
Sbjct: 480 -GLSIIGNIQQQGFRVVYDLASSRVGFAPGGCA 485

[Close]

BLAST of Chy6G115450 vs. ExPASy TrEMBL
Match: A0A5A7U1M2 (Aspartic proteinase PCS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold363G00330 PE=3 SV=1)

HSP 1 Score: 811.2 bits (2094), Expect = 2.0e-231
Identity = 403/430 (93.72%), Postives = 417/430 (96.98%), Query Frame = 0


Query: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTTPLYSSQLYAKRPSSYGSFKLPFK 60
           MLLILFSLSLFTL FSQSNS+SLPFPLSLSEKPSN +P+Y SQLYAK+PSS+GSFKLPFK
Sbjct: 1   MLLILFSLSLFTLPFSQSNSVSLPFPLSLSEKPSNISPIYGSQLYAKKPSSHGSFKLPFK 60

Query: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTASFDPSLSS 120
           YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHD K+KK+LPPLPKPKTASFDPSLSS
Sbjct: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHD-KVKKKLPPLPKPKTASFDPSLSS 120

Query: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLST 180
           SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKF+ SNSLST
Sbjct: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFSLSNSLST 180

Query: 181 PPVILGCAQASTENRGILGMNHGRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGDNPNS 240
           PPVILGCAQASTENRGILGMN GRLSFISQAKISKFSYCVP+RTGSNPTGLFYLGDNPNS
Sbjct: 181 PPVILGCAQASTENRGILGMNKGRLSFISQAKISKFSYCVPARTGSNPTGLFYLGDNPNS 240

Query: 241 SKFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKLLNISPATFKPDAGGSGQTMIDS 300
           S+FKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGK LNISPA FKPDAGGSGQTMIDS
Sbjct: 241 SRFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKRLNISPAAFKPDAGGSGQTMIDS 300

Query: 301 GSDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDAGVTAEVGRRIGGMSFEF 360
           GSDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDA VTAEVGRRIGG+SFEF
Sbjct: 301 GSDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDARVTAEVGRRIGGISFEF 360

Query: 361 ENGVEIFVGRGEEVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRVG 420
           +NGVEI VGRGE VLTEVEKGVKCVG GRSERLGIGSNIIGTVHQQNMWVEYDL N+R+G
Sbjct: 361 DNGVEILVGRGEGVLTEVEKGVKCVGFGRSERLGIGSNIIGTVHQQNMWVEYDLTNRRIG 420

Query: 421 FGGAECSRLK 431
           FGGAECSRLK
Sbjct: 421 FGGAECSRLK 429

[Close]

BLAST of Chy6G115450 vs. ExPASy TrEMBL
Match: A0A1S3C4D2 (aspartic proteinase PCS1 OS=Cucumis melo OX=3656 GN=LOC103496869 PE=3 SV=1)

HSP 1 Score: 811.2 bits (2094), Expect = 2.0e-231
Identity = 403/430 (93.72%), Postives = 417/430 (96.98%), Query Frame = 0


Query: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTTPLYSSQLYAKRPSSYGSFKLPFK 60
           MLLILFSLSLFTL FSQSNS+SLPFPLSLSEKPSN +P+Y SQLYAK+PSS+GSFKLPFK
Sbjct: 1   MLLILFSLSLFTLPFSQSNSVSLPFPLSLSEKPSNISPIYGSQLYAKKPSSHGSFKLPFK 60

Query: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTASFDPSLSS 120
           YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHD K+KK+LPPLPKPKTASFDPSLSS
Sbjct: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHD-KVKKKLPPLPKPKTASFDPSLSS 120

Query: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLST 180
           SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKF+ SNSLST
Sbjct: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFSLSNSLST 180

Query: 181 PPVILGCAQASTENRGILGMNHGRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGDNPNS 240
           PPVILGCAQASTENRGILGMN GRLSFISQAKISKFSYCVP+RTGSNPTGLFYLGDNPNS
Sbjct: 181 PPVILGCAQASTENRGILGMNKGRLSFISQAKISKFSYCVPARTGSNPTGLFYLGDNPNS 240

Query: 241 SKFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKLLNISPATFKPDAGGSGQTMIDS 300
           S+FKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGK LNISPA FKPDAGGSGQTMIDS
Sbjct: 241 SRFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKRLNISPAAFKPDAGGSGQTMIDS 300

Query: 301 GSDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDAGVTAEVGRRIGGMSFEF 360
           GSDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDA VTAEVGRRIGG+SFEF
Sbjct: 301 GSDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDARVTAEVGRRIGGISFEF 360

Query: 361 ENGVEIFVGRGEEVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRVG 420
           +NGVEI VGRGE VLTEVEKGVKCVG GRSERLGIGSNIIGTVHQQNMWVEYDL N+R+G
Sbjct: 361 DNGVEILVGRGEGVLTEVEKGVKCVGFGRSERLGIGSNIIGTVHQQNMWVEYDLTNRRIG 420

Query: 421 FGGAECSRLK 431
           FGGAECSRLK
Sbjct: 421 FGGAECSRLK 429

[Close]

BLAST of Chy6G115450 vs. ExPASy TrEMBL
Match: A0A6J1J2U3 (aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111482170 PE=3 SV=1)

HSP 1 Score: 693.3 bits (1788), Expect = 6.2e-196
Identity = 354/430 (82.33%), Postives = 379/430 (88.14%), Query Frame = 0


Query: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTTPLYSSQLYAKRPSSYGSFKLPFK 60
           MLL LF +SL +LSFS S+SL L FPLSLS++PS  +PL+SS       SSYGS KLPFK
Sbjct: 1   MLLSLFYISLLSLSFSHSHSLPLSFPLSLSKRPSPVSPLFSSL------SSYGSVKLPFK 60

Query: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTASFDPSLSS 120
           Y STALVVSLPIGTPPQPTDLVLDTGSQLSWIQCH K  KK L    KPKTASFDPSLSS
Sbjct: 61  Y-STALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHRKLHKKLL----KPKTASFDPSLSS 120

Query: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLST 180
           SFSLLPCNHP+CKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNS +T
Sbjct: 121 SFSLLPCNHPLCKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSRTT 180

Query: 181 PPVILGCAQASTENRGILGMNHGRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGDNPNS 240
           PPVILGCAQASTENRGILGMN GRLSF+SQAKISKFSYCVP RTG + TGLFYLGDNPNS
Sbjct: 181 PPVILGCAQASTENRGILGMNTGRLSFVSQAKISKFSYCVPGRTGPDSTGLFYLGDNPNS 240

Query: 241 SKFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKLLNISPATFKPDAGGSGQTMIDS 300
           + FKY+++LTFP+SQ SPNLDPLAYTLP+KGIKIAG  LNIS A FKPD  GSGQTMIDS
Sbjct: 241 ANFKYISLLTFPKSQRSPNLDPLAYTLPLKGIKIAGNRLNISSAVFKPDRSGSGQTMIDS 300

Query: 301 GSDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDAGVTAEVGRRIGGMSFEF 360
           GSDLTYLVDEAYEKVKEE+V+LVG  MKKGY YAAVADMCF+ G TAEVGRRI  MSFEF
Sbjct: 301 GSDLTYLVDEAYEKVKEEIVKLVGPLMKKGYEYAAVADMCFNDGETAEVGRRIRDMSFEF 360

Query: 361 ENGVEIFVGRGEEVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRVG 420
           ENGVEI VG+GE VLTEVEKGVKCVG GRS RLGI SNIIGTVHQ+N WVEYDLAN+R+G
Sbjct: 361 ENGVEISVGKGEGVLTEVEKGVKCVGFGRSGRLGIASNIIGTVHQKNTWVEYDLANRRIG 419

Query: 421 FGGAECSRLK 431
           FGGA+CSRLK
Sbjct: 421 FGGADCSRLK 419

[Close]

BLAST of Chy6G115450 vs. ExPASy TrEMBL
Match: A0A6J1F8Z2 (aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111441919 PE=3 SV=1)

HSP 1 Score: 688.3 bits (1775), Expect = 2.0e-194
Identity = 353/434 (81.34%), Postives = 380/434 (87.56%), Query Frame = 0


Query: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKP----SNTTPLYSSQLYAKRPSSYGSFK 60
           MLL LF LSL +LSFS S+SLSL FPLSLS++P    S+ +PL+SS       S+YGS K
Sbjct: 1   MLLSLFYLSLLSLSFSHSHSLSLSFPLSLSKRPSPVSSSVSPLFSSL------SAYGSVK 60

Query: 61  LPFKYSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTASFDP 120
           LPFKY STALVVSLPIGTPPQPTDLVLDTGSQLSWIQCH K  KK L    KPKT SFDP
Sbjct: 61  LPFKY-STALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHRKVHKKLL----KPKTTSFDP 120

Query: 121 SLSSSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSN 180
           SLSSSFSLLPCNHP+CKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSN
Sbjct: 121 SLSSSFSLLPCNHPLCKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSN 180

Query: 181 SLSTPPVILGCAQASTENRGILGMNHGRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGD 240
           S +TPPVILGCAQASTENRGILGMN GRLSF+SQAKISKFSYCVP RTG + TGLFYLGD
Sbjct: 181 SRTTPPVILGCAQASTENRGILGMNTGRLSFVSQAKISKFSYCVPGRTGPDSTGLFYLGD 240

Query: 241 NPNSSKFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKLLNISPATFKPDAGGSGQT 300
           NPNS+ FKY+++LTFP+SQ SPNLDPLAYTLP+KGIKIAG  LNIS A FKPD  GSGQT
Sbjct: 241 NPNSANFKYISLLTFPKSQRSPNLDPLAYTLPLKGIKIAGNRLNISSAVFKPDRSGSGQT 300

Query: 301 MIDSGSDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDAGVTAEVGRRIGGM 360
           MIDSGSDLTYLVDEAYEKVKEE+V+LVG  MKKGY YAAVADMCF+ G TAEVGRRI  M
Sbjct: 301 MIDSGSDLTYLVDEAYEKVKEEIVKLVGPLMKKGYEYAAVADMCFNDGETAEVGRRIRDM 360

Query: 361 SFEFENGVEIFVGRGEEVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLAN 420
           SFEFENGVEI VG+GE VLTEVEKGVKCVG GRS RLGI SNIIGTVHQ+N W+EYDLAN
Sbjct: 361 SFEFENGVEISVGKGEGVLTEVEKGVKCVGFGRSGRLGIASNIIGTVHQKNTWIEYDLAN 420

Query: 421 KRVGFGGAECSRLK 431
           +R+GFGGA+CSRLK
Sbjct: 421 RRIGFGGADCSRLK 423

[Close]

BLAST of Chy6G115450 vs. ExPASy TrEMBL
Match: A0A0A0L6V5 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G188350 PE=3 SV=1)

HSP 1 Score: 626.3 bits (1614), Expect = 9.3e-176
Identity = 332/430 (77.21%), Postives = 335/430 (77.91%), Query Frame = 0


Query: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTTPLYSSQLYAKRPSSYGSFKLPFK 60
           MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNT P YSSQLYAKRPSSYGSFKLPFK
Sbjct: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPFK 60

Query: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTASFDPSLSS 120
           YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKK+KKRLPPLPKPKTASFDPSLSS
Sbjct: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLSS 120

Query: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLST 180
           SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFS SLST
Sbjct: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLST 180

Query: 181 PPVILGCAQASTENRGILGMNHGRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGDNPNS 240
           PPVILGCAQASTENR                                             
Sbjct: 181 PPVILGCAQASTENR--------------------------------------------- 240

Query: 241 SKFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKLLNISPATFKPDAGGSGQTMIDS 300
                                                      A FKPDAGGSGQTMIDS
Sbjct: 241 -------------------------------------------AAFKPDAGGSGQTMIDS 300

Query: 301 GSDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDAGVTAEVGRRIGGMSFEF 360
           GSDLTYLVDEAYEKVKEEVVRLVGA MKKGYVYA VADMCFDAGVTAEVGRRIGG+SFEF
Sbjct: 301 GSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYADVADMCFDAGVTAEVGRRIGGISFEF 342

Query: 361 ENGVEIFVGRGEEVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRVG 420
           +NGVEIFVGRGE VLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRVG
Sbjct: 361 DNGVEIFVGRGEGVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRVG 342

Query: 421 FGGAECSRLK 431
           FGGAECSRLK
Sbjct: 421 FGGAECSRLK 342

[Close]

BLAST of Chy6G115450 vs. NCBI nr
Match: XP_004140731.3 (aspartic proteinase PCS1 [Cucumis sativus] >KAE8650484.1 hypothetical protein Csa_011640 [Cucumis sativus])

HSP 1 Score: 828 bits (2138), Expect = 4.70e-302
Identity = 417/430 (96.98%), Postives = 419/430 (97.44%), Query Frame = 0


Query: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTTPLYSSQLYAKRPSSYGSFKLPFK 60
           MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNT P YSSQLYAKRPSSYGSFKLPFK
Sbjct: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPFK 60

Query: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTASFDPSLSS 120
           YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTASFDPSLSS
Sbjct: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTASFDPSLSS 120

Query: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLST 180
           SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFS SLST
Sbjct: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLST 180

Query: 181 PPVILGCAQASTENRGILGMNHGRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGDNPNS 240
           PPVILGCAQASTENRGILGMN GRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGDNPNS
Sbjct: 181 PPVILGCAQASTENRGILGMNRGRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGDNPNS 240

Query: 241 SKFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKLLNISPATFKPDAGGSGQTMIDS 300
           SKFKYVTMLTFPESQSSPNLDPLAYTLPMK IKIAGK LNI PA FKPDAGGSGQTMIDS
Sbjct: 241 SKFKYVTMLTFPESQSSPNLDPLAYTLPMKAIKIAGKRLNIPPAAFKPDAGGSGQTMIDS 300

Query: 301 GSDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDAGVTAEVGRRIGGMSFEF 360
           GSDLTYLVDEAYEKVKEEVVRLVGA MKKGYVYA VADMCFDAGVTAEVGRRIGG+SFEF
Sbjct: 301 GSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYADVADMCFDAGVTAEVGRRIGGISFEF 360

Query: 361 ENGVEIFVGRGEEVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRVG 420
           +NGVEIFVGRGE VLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRVG
Sbjct: 361 DNGVEIFVGRGEGVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRVG 420

Query: 421 FGGAECSRLK 430
           FGGAECSRLK
Sbjct: 421 FGGAECSRLK 430

[Close]

BLAST of Chy6G115450 vs. NCBI nr
Match: XP_011651212.2 (aspartic proteinase PCS1 [Cucumis sativus] >KAE8650483.1 hypothetical protein Csa_010014 [Cucumis sativus])

HSP 1 Score: 813 bits (2101), Expect = 2.12e-296
Identity = 409/431 (94.90%), Postives = 416/431 (96.52%), Query Frame = 0


Query: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTTPLY-SSQLYAKRPSSYGSFKLPF 60
           MLLILFSLSLFTLSFSQSNSLSLPFPLSL+EKPSN TPLY SSQLY K+PSS+G FKLPF
Sbjct: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLTEKPSNITPLYYSSQLYVKKPSSHGPFKLPF 60

Query: 61  KYSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTASFDPSLS 120
           KYSS+ALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKK+KKRLPPLPKPKTA+FDPSLS
Sbjct: 61  KYSSSALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTATFDPSLS 120

Query: 121 SSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLS 180
           SSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLS
Sbjct: 121 SSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLS 180

Query: 181 TPPVILGCAQASTENRGILGMNHGRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGDNPN 240
           TPPVILGCAQ STENRGILGMNHGRLSFISQAKISKFSYCVPSRTG NPTGLFYLGDNPN
Sbjct: 181 TPPVILGCAQGSTENRGILGMNHGRLSFISQAKISKFSYCVPSRTGPNPTGLFYLGDNPN 240

Query: 241 SSKFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKLLNISPATFKPDAGGSGQTMID 300
           SSKFKYVTMLTFPESQSSPNLDPLAYTLPMK IKIAGK LNI PA FKPDAGGSGQTMID
Sbjct: 241 SSKFKYVTMLTFPESQSSPNLDPLAYTLPMKAIKIAGKRLNIPPAAFKPDAGGSGQTMID 300

Query: 301 SGSDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDAGVTAEVGRRIGGMSFE 360
           SGSDLTYLVDEAYEKVKEEVVRLVGA MKKGYVYAAVADMCFDAGVT EVGRRIG MSFE
Sbjct: 301 SGSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYAAVADMCFDAGVTVEVGRRIGDMSFE 360

Query: 361 FENGVEIFVGRGEEVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRV 420
           F+NGVEIFVGRGE VLTEVEKGVKCVGIGRS RLGIGSNIIGTVHQQNMWVEYDLANKRV
Sbjct: 361 FDNGVEIFVGRGEGVLTEVEKGVKCVGIGRSGRLGIGSNIIGTVHQQNMWVEYDLANKRV 420

Query: 421 GFGGAECSRLK 430
           GFGGAECSRLK
Sbjct: 421 GFGGAECSRLK 431

[Close]

BLAST of Chy6G115450 vs. NCBI nr
Match: XP_008457122.1 (PREDICTED: aspartic proteinase PCS1 [Cucumis melo] >KAA0047379.1 aspartic proteinase PCS1 [Cucumis melo var. makuwa] >TYK14056.1 aspartic proteinase PCS1 [Cucumis melo var. makuwa])

HSP 1 Score: 806 bits (2083), Expect = 1.09e-293
Identity = 403/430 (93.72%), Postives = 417/430 (96.98%), Query Frame = 0


Query: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTTPLYSSQLYAKRPSSYGSFKLPFK 60
           MLLILFSLSLFTL FSQSNS+SLPFPLSLSEKPSN +P+Y SQLYAK+PSS+GSFKLPFK
Sbjct: 1   MLLILFSLSLFTLPFSQSNSVSLPFPLSLSEKPSNISPIYGSQLYAKKPSSHGSFKLPFK 60

Query: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTASFDPSLSS 120
           YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDK +KK+LPPLPKPKTASFDPSLSS
Sbjct: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDK-VKKKLPPLPKPKTASFDPSLSS 120

Query: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLST 180
           SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKF+ SNSLST
Sbjct: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFSLSNSLST 180

Query: 181 PPVILGCAQASTENRGILGMNHGRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGDNPNS 240
           PPVILGCAQASTENRGILGMN GRLSFISQAKISKFSYCVP+RTGSNPTGLFYLGDNPNS
Sbjct: 181 PPVILGCAQASTENRGILGMNKGRLSFISQAKISKFSYCVPARTGSNPTGLFYLGDNPNS 240

Query: 241 SKFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKLLNISPATFKPDAGGSGQTMIDS 300
           S+FKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGK LNISPA FKPDAGGSGQTMIDS
Sbjct: 241 SRFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKRLNISPAAFKPDAGGSGQTMIDS 300

Query: 301 GSDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDAGVTAEVGRRIGGMSFEF 360
           GSDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDA VTAEVGRRIGG+SFEF
Sbjct: 301 GSDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDARVTAEVGRRIGGISFEF 360

Query: 361 ENGVEIFVGRGEEVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRVG 420
           +NGVEI VGRGE VLTEVEKGVKCVG GRSERLGIGSNIIGTVHQQNMWVEYDL N+R+G
Sbjct: 361 DNGVEILVGRGEGVLTEVEKGVKCVGFGRSERLGIGSNIIGTVHQQNMWVEYDLTNRRIG 420

Query: 421 FGGAECSRLK 430
           FGGAECSRLK
Sbjct: 421 FGGAECSRLK 429

[Close]

BLAST of Chy6G115450 vs. NCBI nr
Match: XP_038878004.1 (aspartic proteinase PCS1 [Benincasa hispida])

HSP 1 Score: 731 bits (1888), Expect = 4.62e-264
Identity = 378/430 (87.91%), Postives = 393/430 (91.40%), Query Frame = 0


Query: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTTPLYSSQLYAKRPSSYGSFKLPFK 60
           +L++ FSLSLF+LSFSQSNSLSL FPLSLS+K SN +PLY S LY+K  SS+GSFKLP K
Sbjct: 2   LLILFFSLSLFSLSFSQSNSLSLSFPLSLSKKTSNISPLYPS-LYSKT-SSHGSFKLPVK 61

Query: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTASFDPSLSS 120
           YS TALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDK  KK    LPKPKTASFDPSLSS
Sbjct: 62  YS-TALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKVKKK----LPKPKTASFDPSLSS 121

Query: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLST 180
           SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSL+T
Sbjct: 122 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLTT 181

Query: 181 PPVILGCAQASTENRGILGMNHGRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGDNPNS 240
           PPVILGCAQASTENRGILGMN GRLSFISQAKISKFSYCVP RT  NPTGLFYLGDNPNS
Sbjct: 182 PPVILGCAQASTENRGILGMNTGRLSFISQAKISKFSYCVPRRTRFNPTGLFYLGDNPNS 241

Query: 241 SKFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKLLNISPATFKPDAGGSGQTMIDS 300
           SKFKYVTMLTFP+SQ SPNLDPLAYTLPMKGIKIAGK LNISPA FKPDA GSGQTMIDS
Sbjct: 242 SKFKYVTMLTFPKSQRSPNLDPLAYTLPMKGIKIAGKRLNISPAFFKPDASGSGQTMIDS 301

Query: 301 GSDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDAGVTAEVGRRIGGMSFEF 360
           GSDLTYL DEAYEKVKEE+VRLVG  MKKGY +AAVADMCF+AG  A VGRRIG MSFEF
Sbjct: 302 GSDLTYLADEAYEKVKEEIVRLVGPMMKKGYEFAAVADMCFNAGEAAVVGRRIGNMSFEF 361

Query: 361 ENGVEIFVGRGEEVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRVG 420
           ENGVEI VG+GE VL EVEKGVKCVGIGRS RLGIGSNIIGTVHQQNMWVEYDLA KR+G
Sbjct: 362 ENGVEILVGKGEGVLAEVEKGVKCVGIGRSGRLGIGSNIIGTVHQQNMWVEYDLAKKRIG 421

Query: 421 FGGAECSRLK 430
           FGGAECSRLK
Sbjct: 422 FGGAECSRLK 424

[Close]

BLAST of Chy6G115450 vs. NCBI nr
Match: XP_022983616.1 (aspartic proteinase PCS1-like [Cucurbita maxima])

HSP 1 Score: 689 bits (1778), Expect = 2.17e-247
Identity = 354/430 (82.33%), Postives = 379/430 (88.14%), Query Frame = 0


Query: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTTPLYSSQLYAKRPSSYGSFKLPFK 60
           MLL LF +SL +LSFS S+SL L FPLSLS++PS  +PL+SS       SSYGS KLPFK
Sbjct: 1   MLLSLFYISLLSLSFSHSHSLPLSFPLSLSKRPSPVSPLFSSL------SSYGSVKLPFK 60

Query: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTASFDPSLSS 120
           YS TALVVSLPIGTPPQPTDLVLDTGSQLSWIQCH K  KK L    KPKTASFDPSLSS
Sbjct: 61  YS-TALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHRKLHKKLL----KPKTASFDPSLSS 120

Query: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLST 180
           SFSLLPCNHP+CKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNS +T
Sbjct: 121 SFSLLPCNHPLCKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSRTT 180

Query: 181 PPVILGCAQASTENRGILGMNHGRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGDNPNS 240
           PPVILGCAQASTENRGILGMN GRLSF+SQAKISKFSYCVP RTG + TGLFYLGDNPNS
Sbjct: 181 PPVILGCAQASTENRGILGMNTGRLSFVSQAKISKFSYCVPGRTGPDSTGLFYLGDNPNS 240

Query: 241 SKFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKLLNISPATFKPDAGGSGQTMIDS 300
           + FKY+++LTFP+SQ SPNLDPLAYTLP+KGIKIAG  LNIS A FKPD  GSGQTMIDS
Sbjct: 241 ANFKYISLLTFPKSQRSPNLDPLAYTLPLKGIKIAGNRLNISSAVFKPDRSGSGQTMIDS 300

Query: 301 GSDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDAGVTAEVGRRIGGMSFEF 360
           GSDLTYLVDEAYEKVKEE+V+LVG  MKKGY YAAVADMCF+ G TAEVGRRI  MSFEF
Sbjct: 301 GSDLTYLVDEAYEKVKEEIVKLVGPLMKKGYEYAAVADMCFNDGETAEVGRRIRDMSFEF 360

Query: 361 ENGVEIFVGRGEEVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRVG 420
           ENGVEI VG+GE VLTEVEKGVKCVG GRS RLGI SNIIGTVHQ+N WVEYDLAN+R+G
Sbjct: 361 ENGVEISVGKGEGVLTEVEKGVKCVGFGRSGRLGIASNIIGTVHQKNTWVEYDLANRRIG 419

Query: 421 FGGAECSRLK 430
           FGGA+CSRLK
Sbjct: 421 FGGADCSRLK 419

[Close]

BLAST of Chy6G115450 vs. TAIR 10
Match: AT5G37540.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 550.8 bits (1418), Expect = 9.6e-157
Identity = 283/436 (64.91%), Postives = 339/436 (77.75%), Query Frame = 0


Query: 2   LLILFSLSLFTLSFSQSNSLSLPFPL-SLSEKPSNTTPLYSSQLYAKR----PSSYGSFK 61
           LL +F    +++S S S+SLSL FPL SL   P+  +  + + L ++R    PSS  +F+
Sbjct: 12  LLYIFFFFCYSVSLSWSSSLSLHFPLTSLRLTPTTNSSSFKTSLLSRRNPSPPSSPYTFR 71

Query: 62  LPFKYSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTASFDP 121
              KY S AL++SLPIGTP Q  +LVLDTGSQLSWIQCH KKIKK LP    P T SFDP
Sbjct: 72  SNIKY-SMALILSLPIGTPSQSQELVLDTGSQLSWIQCHPKKIKKPLP----PPTTSFDP 131

Query: 122 SLSSSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSN 181
           SLSSSFS LPC+HP+CKPRIPDFTLPTSCD NRLCHYSYFYADGT AEGNLV+EKFTFSN
Sbjct: 132 SLSSSFSDLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKFTFSN 191

Query: 182 SLSTPPVILGCAQASTENRGILGMNHGRLSFISQAKISKFSYCVPSRT---GSNPTGLFY 241
           S +TPP+ILGCA+ ST+ +GILGMN GRLSFISQAKISKFSYC+P+R+   G   TG FY
Sbjct: 192 SQTTPPLILGCAKESTDEKGILGMNLGRLSFISQAKISKFSYCIPTRSNRPGLASTGSFY 251

Query: 242 LGDNPNSSKFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKLLNISPATFKPDAGGS 301
           LGDNPNS  FKYV++LTFP+SQ  PNLDPLAYT+P++GI+I  K LNI  + F+PDAGGS
Sbjct: 252 LGDNPNSRGFKYVSLLTFPQSQRMPNLDPLAYTVPLQGIRIGQKRLNIPGSVFRPDAGGS 311

Query: 302 GQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDAGVTAEVGRRI 361
           GQTM+DSGS+ T+LVD AY+KVKEE+VRLVG+++KKGYVY + ADMCFD   + E+GR I
Sbjct: 312 GQTMVDSGSEFTHLVDVAYDKVKEEIVRLVGSRLKKGYVYGSTADMCFDGNHSMEIGRLI 371

Query: 362 GGMSFEFENGVEIFVGRGEEVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYD 421
           G + FEF  GVEI V + + +L  V  G+ CVGIGRS  LG  SNIIG VHQQN+WVE+D
Sbjct: 372 GDLVFEFGRGVEILVEK-QSLLVNVGGGIHCVGIGRSSMLGAASNIIGNVHQQNLWVEFD 431

Query: 422 LANKRVGFGGAECSRL 430
           + N+RVGF  AEC  L
Sbjct: 432 VTNRRVGFSKAECRLL 441

[Close]

BLAST of Chy6G115450 vs. TAIR 10
Match: AT1G66180.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 519.2 bits (1336), Expect = 3.1e-147
Identity = 273/432 (63.19%), Postives = 321/432 (74.31%), Query Frame = 0


Query: 4   ILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTTPLYSSQLYAKRPSSYG---SFKLPFK 63
           + F   L  +S S S SL LP         +N+    +S L  K PS      +F+  FK
Sbjct: 8   LFFFFFLNYVSLSTSLSLHLPLTSLPISTTTNSHRFTTSLLSRKNPSPSSPPYNFRSRFK 67

Query: 64  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTASFDPSLSS 123
           Y S AL++SLPIGTPPQ   +VLDTGSQLSWIQCH    +K+LP  PKPKT SFDPSLSS
Sbjct: 68  Y-SMALIISLPIGTPPQAQQMVLDTGSQLSWIQCH----RKKLP--PKPKT-SFDPSLSS 127

Query: 124 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLST 183
           SFS LPC+HP+CKPRIPDFTLPTSCD NRLCHYSYFYADGT AEGNLV+EK TFSN+  T
Sbjct: 128 SFSTLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKITFSNTEIT 187

Query: 184 PPVILGCAQASTENRGILGMNHGRLSFISQAKISKFSYCVP---SRTGSNPTGLFYLGDN 243
           PP+ILGCA  S+++RGILGMN GRLSF+SQAKISKFSYC+P   +R G  PTG FYLGDN
Sbjct: 188 PPLILGCATESSDDRGILGMNRGRLSFVSQAKISKFSYCIPPKSNRPGFTPTGSFYLGDN 247

Query: 244 PNSSKFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKLLNISPATFKPDAGGSGQTM 303
           PNS  FKYV++LTFPESQ  PNLDPLAYT+PM GI+   K LNIS + F+PDAGGSGQTM
Sbjct: 248 PNSHGFKYVSLLTFPESQRMPNLDPLAYTVPMIGIRFGLKKLNISGSVFRPDAGGSGQTM 307

Query: 304 IDSGSDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDAGVTAEVGRRIGGMS 363
           +DSGS+ T+LVD AY+KV+ E++  VG ++KKGYVY   ADMCFD  V A + R IG + 
Sbjct: 308 VDSGSEFTHLVDAAYDKVRAEIMTRVGRRLKKGYVYGGTADMCFDGNV-AMIPRLIGDLV 367

Query: 364 FEFENGVEIFVGRGEEVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANK 423
           F F  GVEI V + E VL  V  G+ CVGIGRS  LG  SNIIG VHQQN+WVE+D+ N+
Sbjct: 368 FVFTRGVEILVPK-ERVLVNVGGGIHCVGIGRSSMLGAASNIIGNVHQQNLWVEFDVTNR 427

Query: 424 RVGFGGAECSRL 430
           RVGF  A+CSR+
Sbjct: 428 RVGFAKADCSRV 429

[Close]

BLAST of Chy6G115450 vs. TAIR 10
Match: AT2G39710.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 242.3 bits (617), Expect = 7.3e-64
Identity = 158/439 (35.99%), Postives = 239/439 (54.44%), Query Frame = 0


Query: 7   SLSLFTLSFSQSNSLSLPFPLSLSEKPSNTTPLYSSQLYAKRPSSYGSFKLPFKYSSTAL 66
           SLSL + +F + + L L FPL+  +  S    L  S    K P S  S KL F+++ T L
Sbjct: 9   SLSL-SKNFLRISVLLLIFPLTFCKTSSTNQTLLFSLKTQKLPQS-SSDKLSFRHNVT-L 68

Query: 67  VVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTASFDPSLSSSFSLLP 126
            V+L +G PPQ   +VLDTGS+LSW+ C      K+ P L     + F+P  SS++S +P
Sbjct: 69  TVTLAVGDPPQNISMVLDTGSELSWLHC------KKSPNL----GSVFNPVSSSTYSPVP 128

Query: 127 CNHPICKPRIPDFTLPTSCD-QNRLCHYSYFYADGTLAEGNLVREKFTFSNSLSTPPVIL 186
           C+ PIC+ R  D  +P SCD +  LCH +  YAD T  EGNL  E F    S++ P  + 
Sbjct: 129 CSSPICRTRTRDLPIPASCDPKTHLCHVAISYADATSIEGNLAHETFVI-GSVTRPGTLF 188

Query: 187 GC--------AQASTENRGILGMNHGRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGDN 246
           GC        ++   ++ G++GMN G LSF++Q   SKFSYC+   +GS+ +G   LGD 
Sbjct: 189 GCMDSGLSSNSEEDAKSTGLMGMNRGSLSFVNQLGFSKFSYCI---SGSDSSGFLLLGDA 248

Query: 247 PNS--SKFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKLLNISPATFKPDAGGSGQ 306
             S     +Y  ++   +S   P  D +AYT+ ++GI++  K+L++  + F PD  G+GQ
Sbjct: 249 SYSWLGPIQYTPLVL--QSTPLPYFDRVAYTVQLEGIRVGSKILSLPKSVFVPDHTGAGQ 308

Query: 307 TMIDSGSDLTYLVDEAYEKVKEEVVRLVGAKMK----KGYVYAAVADMCFDAGVTAEVGR 366
           TM+DSG+  T+L+   Y  +K E +    + ++      +V+    D+C+  G T     
Sbjct: 309 TMVDSGTQFTFLMGPVYTALKNEFITQTKSVLRLVDDPDFVFQGTMDLCYKVGSTTRPNF 368

Query: 367 RIGGMSFEFENGVEIFVGRGEEVL-------TEVEKGVKCVGIGRSERLGIGSNIIGTVH 424
               M      G E+ V  G+++L       +E ++ V C   G S+ LGI + +IG  H
Sbjct: 369 SGLPMVSLMFRGAEMSVS-GQKLLYRVNGAGSEGKEEVYCFTFGNSDLLGIEAFVIGHHH 427

[Close]

BLAST of Chy6G115450 vs. TAIR 10
Match: AT5G02190.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 241.1 bits (614), Expect = 1.6e-63
Identity = 162/451 (35.92%), Postives = 236/451 (52.33%), Query Frame = 0


Query: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTTPLYSSQLYAKRPSSYGSFKLPFK 60
           +LL+L   +   +S S S+S S  F  S S   S+ T +   +          + KL F 
Sbjct: 10  LLLVLSVRTYKCVSSSSSSSSSFSFS-SFSSSSSSQTLVLPLKTRITPTDHRPTDKLHFH 69

Query: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTASFDPSLSS 120
           ++ T L V+L +GTPPQ   +V+DTGS+LSW++C+           P P   +FDP+ SS
Sbjct: 70  HNVT-LTVTLTVGTPPQNISMVIDTGSELSWLRCNRSS-------NPNP-VNNFDPTRSS 129

Query: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLST 180
           S+S +PC+ P C+ R  DF +P SCD ++LCH +  YAD + +EGNL  E F F NS + 
Sbjct: 130 SYSPIPCSSPTCRTRTRDFLIPASCDSDKLCHATLSYADASSSEGNLAAEIFHFGNSTND 189

Query: 181 PPVILGC--------AQASTENRGILGMNHGRLSFISQAKISKFSYCVPSRTGSNPTGLF 240
             +I GC         +  T+  G+LGMN G LSFISQ    KFSYC+ S T   P G  
Sbjct: 190 SNLIFGCMGSVSGSDPEEDTKTTGLLGMNRGSLSFISQMGFPKFSYCI-SGTDDFP-GFL 249

Query: 241 YLGDNPNSSKFKYVTMLTFPE----SQSSPNLDPLAYTLPMKGIKIAGKLLNISPATFKP 300
            LGD    S F ++T L +      S   P  D +AYT+ + GIK+ GKLL I  +   P
Sbjct: 250 LLGD----SNFTWLTPLNYTPLIRISTPLPYFDRVAYTVQLTGIKVNGKLLPIPKSVLVP 309

Query: 301 DAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVR----LVGAKMKKGYVYAAVADMCF--- 360
           D  G+GQTM+DSG+  T+L+   Y  ++   +     ++       +V+    D+C+   
Sbjct: 310 DHTGAGQTMVDSGTQFTFLLGPVYTALRSHFLNRTNGILTVYEDPDFVFQGTMDLCYRIS 369

Query: 361 DAGVTAEVGRRIGGMSFEFENGVEIFVGRGEEVLTEV------EKGVKCVGIGRSERLGI 420
              + + +  R+  +S  FE G EI V  G+ +L  V         V C   G S+ +G+
Sbjct: 370 PVRIRSGILHRLPTVSLVFE-GAEIAVS-GQPLLYRVPHLTVGNDSVYCFTFGNSDLMGM 429

Query: 421 GSNIIGTVHQQNMWVEYDLANKRVGFGGAEC 427
            + +IG  HQQNMW+E+DL   R+G    EC
Sbjct: 430 EAYVIGHHHQQNMWIEFDLQRSRIGLAPVEC 442

[Close]

BLAST of Chy6G115450 vs. TAIR 10
Match: AT2G03200.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 155.2 bits (391), Expect = 1.2e-37
Identity = 116/395 (29.37%), Postives = 181/395 (45.82%), Query Frame = 0


Query: 46  AKRPSSYGSFKLPFKYSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPP 105
           A +P    + K P    S   ++ L IG P      ++DTGS L W QC      K    
Sbjct: 87  ASKPDDTNNIKAPTHGGSGEFLMELSIGNPAVKYSAIVDTGSDLIWTQC------KPCTE 146

Query: 106 LPKPKTASFDPSLSSSFSLLPCNHPICKPRIPDFTLPTS-CDQNR-LCHYSYFYADGTLA 165
                T  FDP  SSS+S + C+  +C        LP S C++++  C Y Y Y D +  
Sbjct: 147 CFDQPTPIFDPEKSSSYSKVGCSSGLCN------ALPRSNCNEDKDACEYLYTYGDYSST 206

Query: 166 EGNLVREKFTFSNSLSTPPVILGC-----AQASTENRGILGMNHGRLSFISQAKISKFSY 225
            G L  E FTF +  S   +  GC         ++  G++G+  G LS ISQ K +KFSY
Sbjct: 207 RGLLATETFTFEDENSISGIGFGCGVENEGDGFSQGSGLVGLGRGPLSLISQLKETKFSY 266

Query: 226 CVPSRTGSNPTGLFYLGDNPNSSKFKYVTMLTFPESQSSPNL----DPLAYTLPMKGIKI 285
           C+ S   S  +   ++G   +    K    L    +++   L     P  Y L ++GI +
Sbjct: 267 CLTSIEDSEASSSLFIGSLASGIVNKTGASLDGEVTKTMSLLRNPDQPSFYYLELQGITV 326

Query: 286 AGKLLNISPATFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYA 345
             K L++  +TF+    G+G  +IDSG+ +TYL + A++ +KEE    +   +      +
Sbjct: 327 GAKRLSVEKSTFELAEDGTGGMIIDSGTTITYLEETAFKVLKEEFTSRMSLPVDDS--GS 386

Query: 346 AVADMCFDAGVTAEVGRRIGGMSFEFENGVEIFVGRGEEVLTEVEKGVKCVGIGRSERLG 405
              D+CF     A+    +  M F F+ G ++ +     ++ +   GV C+ +G S    
Sbjct: 387 TGLDLCFKLPDAAK-NIAVPKMIFHFK-GADLELPGENYMVADSSTGVLCLAMGSSN--- 446

Query: 406 IGSNIIGTVHQQNMWVEYDLANKRVGFGGAECSRL 430
            G +I G V QQN  V +DL  + V F   EC +L
Sbjct: 447 -GMSIFGNVQQQNFNVLHDLEKETVSFVPTECGKL 461

The following BLAST results are available for this feature:

BLAST of Chy6G115450 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LZL3	2.3e-62	35.92	Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1	[more]
Q766C3	7.2e-40	31.34	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q766C2	5.1e-38	32.09	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q9LS40	7.2e-32	27.55	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LNJ3	3.9e-30	28.75	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]

BLAST of Chy6G115450 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7U1M2	2.0e-231	93.72	Aspartic proteinase PCS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A1S3C4D2	2.0e-231	93.72	aspartic proteinase PCS1 OS=Cucumis melo OX=3656 GN=LOC103496869 PE=3 SV=1	[more]
A0A6J1J2U3	6.2e-196	82.33	aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111482170 PE=3 S...	[more]
A0A6J1F8Z2	2.0e-194	81.34	aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111441919 PE=3...	[more]
A0A0A0L6V5	9.3e-176	77.21	Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G18835...	[more]

BLAST of Chy6G115450 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004140731.3	4.70e-302	96.98	aspartic proteinase PCS1 [Cucumis sativus] >KAE8650484.1 hypothetical protein Cs...	[more]
XP_011651212.2	2.12e-296	94.90	aspartic proteinase PCS1 [Cucumis sativus] >KAE8650483.1 hypothetical protein Cs...	[more]
XP_008457122.1	1.09e-293	93.72	PREDICTED: aspartic proteinase PCS1 [Cucumis melo] >KAA0047379.1 aspartic protei...	[more]
XP_038878004.1	4.62e-264	87.91	aspartic proteinase PCS1 [Benincasa hispida]	[more]
XP_022983616.1	2.17e-247	82.33	aspartic proteinase PCS1-like [Cucurbita maxima]	[more]

BLAST of Chy6G115450 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G37540.1	9.6e-157	64.91	Eukaryotic aspartyl protease family protein	[more]
AT1G66180.1	3.1e-147	63.19	Eukaryotic aspartyl protease family protein	[more]
AT2G39710.1	7.3e-64	35.99	Eukaryotic aspartyl protease family protein	[more]
AT5G02190.1	1.6e-63	35.92	Eukaryotic aspartyl protease family protein	[more]
AT2G03200.1	1.2e-37	29.37	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Aspartic peptidase A1 family	PRINTS	PR00792	PEPSIN	coord: 398..413 score: 25.04 coord: 296..307 score: 28.66 coord: 72..92 score: 47.5
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR47965	ASPARTYL PROTEASE-RELATED	coord: 2..429
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 68..236 e-value: 8.7E-36 score: 123.8
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 264..421 e-value: 2.9E-31 score: 108.4
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 235..430 e-value: 4.6E-38 score: 132.6
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 43..234 e-value: 1.2E-36 score: 128.4
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 63..428
None	No IPR available	PANTHER	PTHR47965:SF51	EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEIN	coord: 2..429
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 81..92
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 66..422 score: 32.757278
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 67..426 e-value: 4.04413E-73 score: 228.301

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy6G115450.1	Chy6G115450.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity