Chy6G112600 (gene) Cucumber (hystrix) v1

Overview
NameChy6G112600
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionPectin lyase-like superfamily protein
LocationchrH06: 4989298 .. 4992446 (+)
RNA-Seq ExpressionChy6G112600
SyntenyChy6G112600
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGATGCTGCTAGTAAGTACGTTTCTTTTTATCTGGGTTTTTTTTTCTTCTTCTTTTCTCATTCTATATACTACTACTACTTGATTCTTTTGTTCTACGAATCTTTTCAACCCCATATGCTATTTTCTATTGATTTCTGAACAAACCCTTTTCGTTTTTTTGATGATTTTGTGTGTGTGTGTGTGTGTGTGTGTGTTTTTGTGAGTTCTTACTCTTGTTTTTCAATGTCTTCCAGCATCATCAGTTTACCTCGGGTTCTTTCTTTTTGCTCTGTTATCTATGTTTTTCTTTACAGATGGTCCAGAAACCCTTTTTGACTTTTCGTGATTTGTATTCTCCCTTCTCTTCCGCTATTACCCTTTTTTCCCCTTCTCTCTCTCTCTCTTTCTCTCCTCTCCTTGAACATTTCCTTGATGTTGATTTTCTGGCAGGTTTCAATTTCTAGATTTGATGTTCTTCTGCATTTTGTTACTTTCACGTTATTGATTTCTCTAATTGTTGATTCTATCTCTCTTTGATCTGCTTTTCTTCTTGTGGGATTAGTTTCATATTCATGTAGGTGTTTTGATTTGCAAGTGGTTATGTCAATCACTTTATTTTTTTCATTTATTCTCGAACTTGAATCTGCAATTGTGGGGATTTGCAAAGTTTTTGTTTTTCAATGTAGTTATGGTTCTAGGTGTATTTGGCTTCCTTTCTTTTCTACCATTGTTGTCTATCTTGTTGTAGTTGGTTAATTTAATCAATTCACGGTGTTGCCCCTGAGTTTCTTTCTTTTGTTTCTTTTGTTTTGTTTTTTTTTCTGTAGGTGGCATTGCTTTTGTTTCTACAATTGAGCAATGCTGCAAAAATAATCGGTGAAGAGCTCGCGGGACAGTGCGATTCTAAACCAACATTAGATCCAAGGCCACACAGTGTTTCTATCTTGGAGTTTGGGGCTGTTGGAGATGGCAAAACTCTTAACACCATTGCCTTCCAAAATGCCATTTTCTATCTTAAATCCTTTGCTGATAAGGGTGGAGCTCAGCTTTATGTTCCACCTGGAAAATGGCTTACTGGAAGCATTAATCTTACCAGCCACCTTACCCTTTTCCTGGAAAAAGGTGCTGTGATTCTTGGCTCTCAGGTATGTTCTTCTTCATGGATTGGTGCTCGAACAAGTAGTTGTTTGTAGGCATCGTTTTGAAACGCATTGATCTTCTCTTTAACAGGACCCATCTCATTGGGAACTTGTCAATCCCTTGCCTTCATATGGGAGAGGTATTGAAGTACCAGGAAAACGATACCGGAGCTTGATAAATGGTTACAATTTACAAGATGTTGTAATAACAGGTTTGTTGCTCTCCTAAAATCTAACCCTTTATTCTGAATATAGTTTGTGGATATGCATTCCTCTGAACTAGTGATATCATGTTGATTTTGGATTTGCTAGGTGATGATGGGGTCATTGATGGACAGGGTTTAGTTTGGTGGAATTGGTTCAGCTCTCATTCATTAAACTACAGCCGCCCTCACCTCGTGGAATTTGAGGATTCTCAATATGTAGTTGTTTCAAACCTCACTTTCTTGAATACTCCTGCATATAACATTCATCCAGTCTATTGCAGGTATGCTATAACAACCAGTGATCAGTTTCTTCTGCCTCTGCCGATTGTGTTCACCTTGTTGATTTGTCTATTTGCTGATTGTCAGAGAATCTGTATTGAATAAAAGTTTTTCATGTGCAGTAACGTCTATGTTTACAACATCTCTGTCTCTGCACCTTCTGAATCTCCCTACACAGTTGGAATAGTCCCAGGTTTGTTATGCTTCAATTTTCAGCATCCATCATTGTTATTTTGTGTGACTATTGTTGATGGGCTCTCTCTCACTCTCACTCACTCTCTCCCTCTCGTTTGATGTGATACATCAATTGGCTGAATGGCTTGAGCACCATTGAAGAATCTCTCTCTTTTATCTCTCACTTTCACATGCACACACACAGCACTTAGTACAAAGAAGAAAATGCCATCAAGAAAGCTATTATAAGCAGTTGACTGTTAACAGATATGTTATAAACTTTAGTTACTATCAACACTTGTTTAACATCATGTAATCATAGAAAAACAATGCCCTCTTATAAAAAAAAAGTCCCAATATACCAAGTTCTTATATTTGGGGCCTGAGGTCTATGGTGATAAATTTTACAGTAAAATTTAAGAAACTTGACACTGTTAAAATGGTGACGTTGGATTCAATTTAGAAATAAGATTCTGTTAACTTAGGAGTTTATGACGCGTAGTGGGATGCCATCATGATCAAAAGGATTTATTGTGGGGCGGAACAAATTTAGACATACAGTACATGATGGCTTCATTAATGATCATCAATTCAAGAATTAAAATTAGTAGTTTGCAATTTATTCTTTCTTGTTTCTAATTAATGAGGTGGAGAAGCATTCTTTTCTAAATAAAGTTTGATTGGTGCTTTCAGATTCCTCCGATCATGTATGCATAGAGGGCTGCAACATTGCCACAGGATATGATGCCATTGCCTTGAAGAGTGGTTGGGATCAGTATGGTATTGCCTATGGCAGACCATCTAAAAACATACACATTAGACGGGTCCACCTTCAGTCATCTTCAGGTTCTTCCATTGCCTTTGGTAGTGAGATGTCTGGTGGGATATCTAATGTTCTTGTGGAGCATGTACAACTGAACAACTCATTTATTGGCATTCAAATTAGAACTACCAAGGGAAGAGGGGGTTACATTAAAGGAATAGTTGTTTCGGATGTTGAAATGGAAAACATATCCACGGCATTCAGTGCCTCTGGTCACTTTGGGTCACATCCGGATGATGAGTATGATCCTAATGCTCTTCCAATTGTGCAGGACATAACCTTACAGAATGTGAGAGGCACCAACATTAAAATTGCTGGAAACTTTTCTGGGATACAAGAATCTCCCTTCACTTCAATTTATTTATCCAACATTACCTTTTCAATCAATTCATCTTCTTCCACTTCTTGGATCTGTTCAGATGTTTCTGGCTTTTCAGAATCTGTGATCCCCCCACCCTGTTCTGATCTCAGTACTCCATATTCAATTTCTTCCTCAGCAGCCTCTCCCCTTGTGAATTCAACTGGAAAGACTGCTGTTTTATGA

mRNA sequence

ATGAGGATGCTGCTAGTGGCATTGCTTTTGTTTCTACAATTGAGCAATGCTGCAAAAATAATCGGTGAAGAGCTCGCGGGACAGTGCGATTCTAAACCAACATTAGATCCAAGGCCACACAGTGTTTCTATCTTGGAGTTTGGGGCTGTTGGAGATGGCAAAACTCTTAACACCATTGCCTTCCAAAATGCCATTTTCTATCTTAAATCCTTTGCTGATAAGGGTGGAGCTCAGCTTTATGTTCCACCTGGAAAATGGCTTACTGGAAGCATTAATCTTACCAGCCACCTTACCCTTTTCCTGGAAAAAGGTGCTGTGATTCTTGGCTCTCAGGACCCATCTCATTGGGAACTTGTCAATCCCTTGCCTTCATATGGGAGAGGTATTGAAGTACCAGGAAAACGATACCGGAGCTTGATAAATGGTTACAATTTACAAGATGTTGTAATAACAGGTGATGATGGGGTCATTGATGGACAGGGTTTAGTTTGGTGGAATTGGTTCAGCTCTCATTCATTAAACTACAGCCGCCCTCACCTCGTGGAATTTGAGGATTCTCAATATGTAGTTGTTTCAAACCTCACTTTCTTGAATACTCCTGCATATAACATTCATCCAGTCTATTGCAGTAACGTCTATGTTTACAACATCTCTGTCTCTGCACCTTCTGAATCTCCCTACACAGTTGGAATAGTCCCAGATTCCTCCGATCATGTATGCATAGAGGGCTGCAACATTGCCACAGGATATGATGCCATTGCCTTGAAGAGTGGTTGGGATCAGTATGGTATTGCCTATGGCAGACCATCTAAAAACATACACATTAGACGGGTCCACCTTCAGTCATCTTCAGGTTCTTCCATTGCCTTTGGTAGTGAGATGTCTGGTGGGATATCTAATGTTCTTGTGGAGCATGTACAACTGAACAACTCATTTATTGGCATTCAAATTAGAACTACCAAGGGAAGAGGGGGTTACATTAAAGGAATAGTTGTTTCGGATGTTGAAATGGAAAACATATCCACGGCATTCAGTGCCTCTGGTCACTTTGGGTCACATCCGGATGATGAGTATGATCCTAATGCTCTTCCAATTGTGCAGGACATAACCTTACAGAATGTGAGAGGCACCAACATTAAAATTGCTGGAAACTTTTCTGGGATACAAGAATCTCCCTTCACTTCAATTTATTTATCCAACATTACCTTTTCAATCAATTCATCTTCTTCCACTTCTTGGATCTGTTCAGATGTTTCTGGCTTTTCAGAATCTGTGATCCCCCCACCCTGTTCTGATCTCAGTACTCCATATTCAATTTCTTCCTCAGCAGCCTCTCCCCTTGTGAATTCAACTGGAAAGACTGCTGTTTTATGA

Coding sequence (CDS)

ATGAGGATGCTGCTAGTGGCATTGCTTTTGTTTCTACAATTGAGCAATGCTGCAAAAATAATCGGTGAAGAGCTCGCGGGACAGTGCGATTCTAAACCAACATTAGATCCAAGGCCACACAGTGTTTCTATCTTGGAGTTTGGGGCTGTTGGAGATGGCAAAACTCTTAACACCATTGCCTTCCAAAATGCCATTTTCTATCTTAAATCCTTTGCTGATAAGGGTGGAGCTCAGCTTTATGTTCCACCTGGAAAATGGCTTACTGGAAGCATTAATCTTACCAGCCACCTTACCCTTTTCCTGGAAAAAGGTGCTGTGATTCTTGGCTCTCAGGACCCATCTCATTGGGAACTTGTCAATCCCTTGCCTTCATATGGGAGAGGTATTGAAGTACCAGGAAAACGATACCGGAGCTTGATAAATGGTTACAATTTACAAGATGTTGTAATAACAGGTGATGATGGGGTCATTGATGGACAGGGTTTAGTTTGGTGGAATTGGTTCAGCTCTCATTCATTAAACTACAGCCGCCCTCACCTCGTGGAATTTGAGGATTCTCAATATGTAGTTGTTTCAAACCTCACTTTCTTGAATACTCCTGCATATAACATTCATCCAGTCTATTGCAGTAACGTCTATGTTTACAACATCTCTGTCTCTGCACCTTCTGAATCTCCCTACACAGTTGGAATAGTCCCAGATTCCTCCGATCATGTATGCATAGAGGGCTGCAACATTGCCACAGGATATGATGCCATTGCCTTGAAGAGTGGTTGGGATCAGTATGGTATTGCCTATGGCAGACCATCTAAAAACATACACATTAGACGGGTCCACCTTCAGTCATCTTCAGGTTCTTCCATTGCCTTTGGTAGTGAGATGTCTGGTGGGATATCTAATGTTCTTGTGGAGCATGTACAACTGAACAACTCATTTATTGGCATTCAAATTAGAACTACCAAGGGAAGAGGGGGTTACATTAAAGGAATAGTTGTTTCGGATGTTGAAATGGAAAACATATCCACGGCATTCAGTGCCTCTGGTCACTTTGGGTCACATCCGGATGATGAGTATGATCCTAATGCTCTTCCAATTGTGCAGGACATAACCTTACAGAATGTGAGAGGCACCAACATTAAAATTGCTGGAAACTTTTCTGGGATACAAGAATCTCCCTTCACTTCAATTTATTTATCCAACATTACCTTTTCAATCAATTCATCTTCTTCCACTTCTTGGATCTGTTCAGATGTTTCTGGCTTTTCAGAATCTGTGATCCCCCCACCCTGTTCTGATCTCAGTACTCCATATTCAATTTCTTCCTCAGCAGCCTCTCCCCTTGTGAATTCAACTGGAAAGACTGCTGTTTTATGA

Protein sequence

MRMLLVALLLFLQLSNAAKIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVNPLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHLVEFEDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLVEHVQLNNSFIGIQIRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDPNALPIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSESVIPPPCSDLSTPYSISSSAASPLVNSTGKTAVL*
Homology
BLAST of Chy6G112600 vs. ExPASy Swiss-Prot
Match: A7PZL3 (Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 1.0e-113
Identity = 208/431 (48.26%), Postives = 278/431 (64.50%), Query Frame = 0

Query: 4   LLVALLLFLQLSNAAKIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQN 63
           +LVA++L L +S      G  L  +      +  R HS S+++FG VGDG+TLNT AFQ+
Sbjct: 27  VLVAVVLLLSVSRGECRKGRIL--EALEYSAISCRAHSASLVDFGGVGDGQTLNTKAFQD 86

Query: 64  AIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVNPLP 123
           A+  L  +  +GGAQLYVP GKWLTGS +LTSH TLFL + AV+L SQD S W ++ PLP
Sbjct: 87  AVSELSKYGSEGGAQLYVPAGKWLTGSFSLTSHFTLFLHRDAVLLASQDISQWPVIKPLP 146

Query: 124 SYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHLVEF 183
           SYGRG +    RY SLI G NL DV+ITGD+G IDGQG +WW  F    L Y+RP+L+E 
Sbjct: 147 SYGRGRDAAAGRYTSLIFGTNLTDVIITGDNGTIDGQGGLWWQRFHGGKLKYTRPYLIEL 206

Query: 184 EDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEG 243
             S  + +SNLT LN+P++N+HPVY  N+ +  I++ AP  SP T GI PDS  +  IE 
Sbjct: 207 MYSADIQISNLTLLNSPSWNVHPVYSRNILIQGITILAPVRSPNTDGINPDSCTNTRIED 266

Query: 244 CNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLV 303
           C I +G D +A+KSGWD+YGIAYG P+K + IRR+   S   + IA GSEMSGGI +V  
Sbjct: 267 CYIVSGDDCVAVKSGWDEYGIAYGMPTKQLVIRRLTCISPYSAVIALGSEMSGGIQDVRA 326

Query: 304 EHVQLNNSFIGIQIRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDPNAL 363
           E +   NS  GI+I+T  GRGGY+K I V  + M+ +  AF  +G++GSH D+ YDP A 
Sbjct: 327 EDIVAINSESGIRIKTGIGRGGYVKDIYVRGMTMKTMKWAFWMTGNYGSHADNHYDPKAF 386

Query: 364 PIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINS-SSSTSWICSDVSGFS 423
           P++Q I  +++   N+ +A    GI   PFT I +SN+T  + + +    W C+DV G S
Sbjct: 387 PVIQGINYRDMVAENVSMAARLEGIPSDPFTGICISNVTIHLAAKAKKVPWTCTDVEGIS 446

Query: 424 ESVIPPPCSDL 434
             V P PCS L
Sbjct: 447 SGVTPTPCSTL 455

BLAST of Chy6G112600 vs. ExPASy Swiss-Prot
Match: Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 1.8e-25
Identity = 113/407 (27.76%), Postives = 182/407 (44.72%), Query Frame = 0

Query: 40  HSVSILEFGAVGDGKTLNTIAFQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTL 99
           +++ + +FGAVGDG T ++ AF  A  +    +  G  Q  VP G        +T  L  
Sbjct: 22  NALDVTQFGAVGDGVTDDSQAFLKA--WEAVCSGTGDGQFVVPAG--------MTFMLQP 81

Query: 100 FLEKGAVILGSQDPSHWELVNPLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDG 159
              +G+       P   +++  L +  +G     K    L    +++ +VI G DG I+G
Sbjct: 82  LKFQGSC---KSTPVFVQMLGKLVAPSKGNWKGDKDQWILFT--DIEGLVIEG-DGEING 141

Query: 160 QGLVWWNWFSSHSLNYSRPHLVEFEDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISV 219
           QG  WW          SRP  ++F     + +S LT L++P  +IH   C+ V + ++ +
Sbjct: 142 QGSSWWEH------KGSRPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRI 201

Query: 220 SAPSESPYTVGIVPDSSDHVCIEGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVH 279
           +AP  SP T GI   +S +V I+ C IATG D IA+ SG           + NIHI  + 
Sbjct: 202 NAPESSPNTDGIDVGASSNVVIQDCIIATGDDCIAINSG-----------TSNIHISGI- 261

Query: 280 LQSSSGSSIAFGSEMSGG----ISNVLVEHVQLNNSFIGIQIRTTKGRGGYIKGIVVSDV 339
                G  I+ GS    G    + NV V++     +  G +I+T +G  GY + I  + +
Sbjct: 262 -DCGPGHGISIGSLGKDGETATVENVCVQNCNFRGTMNGARIKTWQGGSGYARMITFNGI 321

Query: 340 EMENISTAFSASGHF-GSHPDDEYDPNALPI-VQDITLQNVRGTNIKIAG-NFSGIQESP 399
            ++N+         + G   D+  D  +  + V  +   N  GT+    G +F   +  P
Sbjct: 322 TLDNVENPIIIDQFYNGGDSDNAKDRKSSAVEVSKVVFSNFIGTSKSEYGVDFRCSERVP 381

Query: 400 FTSIYLSNITFSINSSSS---TSWICSDVSGFSESVIPP-PCSDLST 436
            T I+L ++     SS S       C +V G S   +P   C +LST
Sbjct: 382 CTEIFLRDMKIETASSGSGQVAQGQCLNVRGASTIAVPGLECLELST 393

BLAST of Chy6G112600 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 118.2 bits (295), Expect = 2.4e-25
Identity = 108/404 (26.73%), Postives = 192/404 (47.52%), Query Frame = 0

Query: 39  PHSVSILEFGAVGDGKTLNTIAFQNAIFYLKSFADKGGAQLYVPPGK-WLTGSINLTS-- 98
           P +VS+ +FGA GDGKT +T AF NA  + K+ +  G   L VP G  +L  SI LT   
Sbjct: 65  PTTVSVSDFGAKGDGKTDDTQAFVNA--WKKACSSNGAVNLLVPKGNTYLLKSIQLTGPC 124

Query: 99  HLTLFLEKGAVILGSQDPSHWELVNPLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDG 158
           +  L ++    +  SQ  S ++ ++      + I   G      +N  ++      GD G
Sbjct: 125 NSILTVQIFGTLSASQKRSDYKDIS------KWIMFDG------VNNLSVDG----GDTG 184

Query: 159 VIDGQGLVWWNWFSSHSLNYSR-----PHLVEFEDSQYVVVSNLTFLNTPAYNIHPVYCS 218
           V+DG G  WW   +S   N ++     P  + F +S+ ++V NL   N     I    CS
Sbjct: 185 VVDGNGETWWQ--NSCKRNKAKPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCS 244

Query: 219 NVYVYNISVSAPSESPYTVGIVPDSSDHVCIEGCNIATGYDAIALKSGWDQYGIAYGRPS 278
           NV V N+ V+AP++SP T GI   ++ ++ +    I TG D I+++SG           S
Sbjct: 245 NVQVSNVVVTAPADSPNTDGIHITNTQNIRVSESIIGTGDDCISIESG-----------S 304

Query: 279 KNIHIRRVHLQSSSGSSI-AFGSEMSGG-ISNVLVEHVQLNNSFIGIQIRTTKGRGGYIK 338
           +N+ I  +      G SI + G + S   +S V V+  +L+ +  G++I+T +G  G   
Sbjct: 305 QNVQINDITCGPGHGISIGSLGDDNSKAFVSGVTVDGAKLSGTDNGVRIKTYQGGSGTAS 364

Query: 339 GIVVSDVEMENISTAFSASGHFGSHPDDEYDPNALPIVQDITLQNVRGTNI-KIAGNFSG 398
            I+  +++M+N+         +        + +A+  V+++  +++ GT+  + A  F+ 
Sbjct: 365 NIIFQNIQMDNVKNPIIIDQDYCDKSKCTTEKSAVQ-VKNVVYRDISGTSASENAITFNC 424

Query: 399 IQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSESVIPPPCS 432
            +  P   I L  +  +I    +T   C++ +   +  + P C+
Sbjct: 425 SKNYPCQGIVLDRV--NIKGGKAT---CTNANVVDKGAVLPQCN 431

BLAST of Chy6G112600 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 1.0e-23
Identity = 106/406 (26.11%), Postives = 180/406 (44.33%), Query Frame = 0

Query: 43  SILEFGAVGDGKTLNTIAFQNAIFYLKSFADKGGAQLYVPPG-KWLTGSINLTS----HL 102
           ++ ++GAVGDGK   T AF  A    ++   K  A L VP   K++  ++        H 
Sbjct: 60  NVEKYGAVGDGKHDCTEAFSTA---WQAACKKPSAMLLVPGNKKFVVNNLFFNGPCQPHF 119

Query: 103 TLFLEKGAVILGSQDPSHWELVNPLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVI 162
           T  ++   +I   Q+P+ W                 K  R  +    L    + G  GVI
Sbjct: 120 TFKVD--GIIAAYQNPASW-----------------KNNRIWLQFAKLTGFTLMG-KGVI 179

Query: 163 DGQGLVWW----NWFSSHSL--NYSRPHLVEFEDSQYVVVSNLTFLNTPAYNIHPVYCSN 222
           DGQG  WW     W +   +  +  RP  ++F+ S  +++  L  +N+P +++    C  
Sbjct: 180 DGQGKQWWAGQCKWVNGREICNDRDRPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEG 239

Query: 223 VYVYNISVSAPSESPYTVGIVPDSSDHVCIEGCNIATGYDAIALKSGWDQYGIAYGRPSK 282
           V +  IS++AP +SP T GI   +S +  ++   I TG D +A+ +G           S 
Sbjct: 240 VKIIGISITAPRDSPNTDGIDIFASKNFHLQKNTIGTGDDCVAIGTG-----------SS 299

Query: 283 NIHIRRVHLQSSSGSSI-AFGSEMS-GGISNVLVEHVQLNNSFIGIQIRTTKGRGGYIKG 342
           NI I  +      G SI + G E S   +S V V   +  ++  G++I+T +G  G    
Sbjct: 300 NIVIEDLICGPGHGISIGSLGRENSRAEVSYVHVNGAKFIDTQNGLRIKTWQGGSGMASH 359

Query: 343 IVVSDVEMENISTAFSASGHFGSHPDDEYDPNALPIVQDITLQNVRGTNIKIAGNFSGIQ 402
           I+  +VEM N       +  + +      +  +   +QD+T +N+RGT+   A       
Sbjct: 360 IIYENVEMINSENPILINQFYCTSASACQNQRSAVQIQDVTYKNIRGTSATAAAIQLKCS 419

Query: 403 ES-PFTSIYLSNITFSINSSSSTSWICSDVSGFSESVIPPPCSDLS 435
           +S P   I LS+I+  + S    S +  + +G+    + P C +LS
Sbjct: 420 DSMPCKDIKLSDISLKLTSGKIASCLNDNANGYFSGHVIPACKNLS 431

BLAST of Chy6G112600 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 2.1e-21
Identity = 96/403 (23.82%), Postives = 175/403 (43.42%), Query Frame = 0

Query: 41  SVSILEFGAVGDGKTLNTIAFQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLT-- 100
           +V++ +FGA GDG+  +T AF+ A  +  + +    A L VP   +L   I+ +      
Sbjct: 89  TVNVDDFGAKGDGRD-DTKAFEKA--WKAACSSTSSAVLLVPKKNYLVRPISFSGPCKSG 148

Query: 101 LFLEKGAVILGSQDPSHWELVNPLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVID 160
           L ++    I  S D S +                 K  R  +   ++Q++ + G  G I+
Sbjct: 149 LTMQIYGTIEASDDRSDYR----------------KDGRHWLVFDSVQNLRVEG-GGTIN 208

Query: 161 GQGLVWWNWFSSHSLNYS-----RPHLVEFEDSQYVVVSNLTFLNTPAYNIHPVYCSNVY 220
           G G +WW   +S   N +      P  + F  S++V+V NL   N    ++    C NV 
Sbjct: 209 GNGKIWWQ--NSCKTNKALPCKDAPTALTFYKSKHVIVKNLKIENAQQIHVSFDNCVNVQ 268

Query: 221 VYNISVSAPSESPYTVGIVPDSSDHVCIEGCNIATGYDAIALKSGWDQYGIAYGRPSKNI 280
             N+ V+AP  SP T GI    + ++ I  C I TG D I++ +G           S+ +
Sbjct: 269 ASNLMVTAPENSPNTDGIHVTGTQNIHISSCVIGTGDDCISIVNG-----------SRKV 328

Query: 281 HIRRVHLQSSSG---SSIAFGSEMSGGISNVLVEHVQLNNSFIGIQIRTTKGRGGYIKGI 340
            +  +      G    S+ +G+     +S+V+V   +L  +  G++I+T +G  G    I
Sbjct: 329 RVNDITCGPGHGISIGSLGYGNS-EAHVSDVVVNGAKLCGTTNGVRIKTWQGGSGSASNI 388

Query: 341 VVSDVEMENISTAFSASGHFGSHPDDEYDPNALPIVQDITLQNVRGT-NIKIAGNFSGIQ 400
              +VEM N+        ++        + ++   V+++  QN++GT    +A  F   +
Sbjct: 389 KFQNVEMHNVENPIIIDQNYCDQDKPCQEQSSAVQVKNVVYQNIKGTCASNVAITFDCSK 448

Query: 401 ESPFTSIYLSNITFSINSSSSTSWICSDVSGFSESVIPPPCSD 433
             P   I L ++   I   ++   +C++V      V+ P C +
Sbjct: 449 RFPCQGIVLEDVDLEIEGGAAAKALCNNVELSETGVVSPHCQE 457

BLAST of Chy6G112600 vs. ExPASy TrEMBL
Match: A0A0A0LAN4 (Pectate_lyase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G165090 PE=3 SV=1)

HSP 1 Score: 916.8 bits (2368), Expect = 3.7e-263
Identity = 456/457 (99.78%), Postives = 456/457 (99.78%), Query Frame = 0

Query: 1   MRMLLVALLLFLQLSNAAKIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIA 60
           MRMLLVALLLFLQLSNAAKIIGEEL GQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIA
Sbjct: 1   MRMLLVALLLFLQLSNAAKIIGEELVGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIA 60

Query: 61  FQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVN 120
           FQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVN
Sbjct: 61  FQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVN 120

Query: 121 PLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHL 180
           PLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHL
Sbjct: 121 PLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHL 180

Query: 181 VEFEDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVC 240
           VEFEDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVC
Sbjct: 181 VEFEDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVC 240

Query: 241 IEGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISN 300
           IEGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISN
Sbjct: 241 IEGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISN 300

Query: 301 VLVEHVQLNNSFIGIQIRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDP 360
           VLVEHVQLNNSFIGIQIRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDP
Sbjct: 301 VLVEHVQLNNSFIGIQIRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDP 360

Query: 361 NALPIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSG 420
           NALPIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSG
Sbjct: 361 NALPIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSG 420

Query: 421 FSESVIPPPCSDLSTPYSISSSAASPLVNSTGKTAVL 458
           FSESVIPPPCSDLSTPYSISSSAASPLVNSTGKTAVL
Sbjct: 421 FSESVIPPPCSDLSTPYSISSSAASPLVNSTGKTAVL 457

BLAST of Chy6G112600 vs. ExPASy TrEMBL
Match: A0A1S3AXL3 (probable polygalacturonase isoform X2 OS=Cucumis melo OX=3656 GN=LOC103483871 PE=3 SV=1)

HSP 1 Score: 912.1 bits (2356), Expect = 9.0e-262
Identity = 453/457 (99.12%), Postives = 455/457 (99.56%), Query Frame = 0

Query: 1   MRMLLVALLLFLQLSNAAKIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIA 60
           MRMLLVALLLFLQLSNAA+IIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIA
Sbjct: 1   MRMLLVALLLFLQLSNAARIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIA 60

Query: 61  FQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVN 120
           FQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVN
Sbjct: 61  FQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVN 120

Query: 121 PLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHL 180
           PLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHL
Sbjct: 121 PLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHL 180

Query: 181 VEFEDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVC 240
           VEFEDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISV APSESPYTVGIVPDSSDHVC
Sbjct: 181 VEFEDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVYAPSESPYTVGIVPDSSDHVC 240

Query: 241 IEGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISN 300
           IEGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISN
Sbjct: 241 IEGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISN 300

Query: 301 VLVEHVQLNNSFIGIQIRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDP 360
           VLVEHVQLNNSFIGI+ RTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDP
Sbjct: 301 VLVEHVQLNNSFIGIEFRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDP 360

Query: 361 NALPIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSG 420
           NALPIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSG
Sbjct: 361 NALPIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSG 420

Query: 421 FSESVIPPPCSDLSTPYSISSSAASPLVNSTGKTAVL 458
           FSESVIPPPCSDLSTPYSISSSAASPLVNSTGKTAVL
Sbjct: 421 FSESVIPPPCSDLSTPYSISSSAASPLVNSTGKTAVL 457

BLAST of Chy6G112600 vs. ExPASy TrEMBL
Match: A0A1S3AX49 (probable polygalacturonase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483871 PE=3 SV=1)

HSP 1 Score: 905.2 bits (2338), Expect = 1.1e-259
Identity = 448/452 (99.12%), Postives = 450/452 (99.56%), Query Frame = 0

Query: 6   VALLLFLQLSNAAKIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQNAI 65
           VALLLFLQLSNAA+IIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQNAI
Sbjct: 7   VALLLFLQLSNAARIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQNAI 66

Query: 66  FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVNPLPSY 125
           FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVNPLPSY
Sbjct: 67  FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVNPLPSY 126

Query: 126 GRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHLVEFED 185
           GRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHLVEFED
Sbjct: 127 GRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHLVEFED 186

Query: 186 SQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEGCN 245
           SQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISV APSESPYTVGIVPDSSDHVCIEGCN
Sbjct: 187 SQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVYAPSESPYTVGIVPDSSDHVCIEGCN 246

Query: 246 IATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLVEH 305
           IATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLVEH
Sbjct: 247 IATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLVEH 306

Query: 306 VQLNNSFIGIQIRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDPNALPI 365
           VQLNNSFIGI+ RTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDPNALPI
Sbjct: 307 VQLNNSFIGIEFRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDPNALPI 366

Query: 366 VQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSESV 425
           VQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSESV
Sbjct: 367 VQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSESV 426

Query: 426 IPPPCSDLSTPYSISSSAASPLVNSTGKTAVL 458
           IPPPCSDLSTPYSISSSAASPLVNSTGKTAVL
Sbjct: 427 IPPPCSDLSTPYSISSSAASPLVNSTGKTAVL 458

BLAST of Chy6G112600 vs. ExPASy TrEMBL
Match: A0A6J1GUW5 (probable polygalacturonase isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111457693 PE=3 SV=1)

HSP 1 Score: 859.4 bits (2219), Expect = 7.0e-246
Identity = 427/458 (93.23%), Postives = 441/458 (96.29%), Query Frame = 0

Query: 2   RMLLVALLLFLQLSNAAKIIGEEL--AGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTI 61
           RML+  LLL LQLSNA KI+GEEL   GQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTI
Sbjct: 3   RMLVALLLLLLQLSNATKIVGEELEVVGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTI 62

Query: 62  AFQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELV 121
           AFQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQ+PSHWELV
Sbjct: 63  AFQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQNPSHWELV 122

Query: 122 NPLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPH 181
           NPLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPH
Sbjct: 123 NPLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPH 182

Query: 182 LVEFEDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHV 241
           LVEFEDS+Y+VVSNLTFLNTPAYNIHPVYCSNVY+YNISVSAPSESP+TVGIVPDSSDHV
Sbjct: 183 LVEFEDSKYIVVSNLTFLNTPAYNIHPVYCSNVYIYNISVSAPSESPHTVGIVPDSSDHV 242

Query: 242 CIEGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGIS 301
           CIEG NIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGIS
Sbjct: 243 CIEGSNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGIS 302

Query: 302 NVLVEHVQLNNSFIGIQIRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYD 361
           NVLVEHVQL+NS   IQ RTTKGRGGYIKGIVVSDVEMENISTAFSASGHFG HPDDE+D
Sbjct: 303 NVLVEHVQLHNSSTAIQFRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGLHPDDEFD 362

Query: 362 PNALPIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVS 421
           PNALPIVQDITLQNVRG+NIKIAGNFSGIQESPFTSIYLSNITF I+SSSS+SWICSDVS
Sbjct: 363 PNALPIVQDITLQNVRGSNIKIAGNFSGIQESPFTSIYLSNITFLISSSSSSSWICSDVS 422

Query: 422 GFSESVIPPPCSDLSTPYSISSSAASPLVNSTGKTAVL 458
           GFS+SVIPPPCSDLS PYSISSSAASPL+NS  KTAVL
Sbjct: 423 GFSDSVIPPPCSDLSHPYSISSSAASPLLNSARKTAVL 460

BLAST of Chy6G112600 vs. ExPASy TrEMBL
Match: A0A6J1GW41 (probable polygalacturonase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111457693 PE=3 SV=1)

HSP 1 Score: 855.9 bits (2210), Expect = 7.7e-245
Identity = 424/452 (93.81%), Postives = 437/452 (96.68%), Query Frame = 0

Query: 8   LLLFLQLSNAAKIIGEEL--AGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQNAI 67
           LLL LQLSNA KI+GEEL   GQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQNAI
Sbjct: 10  LLLLLQLSNATKIVGEELEVVGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQNAI 69

Query: 68  FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVNPLPSY 127
           FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQ+PSHWELVNPLPSY
Sbjct: 70  FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQNPSHWELVNPLPSY 129

Query: 128 GRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHLVEFED 187
           GRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHLVEFED
Sbjct: 130 GRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHLVEFED 189

Query: 188 SQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEGCN 247
           S+Y+VVSNLTFLNTPAYNIHPVYCSNVY+YNISVSAPSESP+TVGIVPDSSDHVCIEG N
Sbjct: 190 SKYIVVSNLTFLNTPAYNIHPVYCSNVYIYNISVSAPSESPHTVGIVPDSSDHVCIEGSN 249

Query: 248 IATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLVEH 307
           IATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLVEH
Sbjct: 250 IATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLVEH 309

Query: 308 VQLNNSFIGIQIRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDPNALPI 367
           VQL+NS   IQ RTTKGRGGYIKGIVVSDVEMENISTAFSASGHFG HPDDE+DPNALPI
Sbjct: 310 VQLHNSSTAIQFRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGLHPDDEFDPNALPI 369

Query: 368 VQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSESV 427
           VQDITLQNVRG+NIKIAGNFSGIQESPFTSIYLSNITF I+SSSS+SWICSDVSGFS+SV
Sbjct: 370 VQDITLQNVRGSNIKIAGNFSGIQESPFTSIYLSNITFLISSSSSSSWICSDVSGFSDSV 429

Query: 428 IPPPCSDLSTPYSISSSAASPLVNSTGKTAVL 458
           IPPPCSDLS PYSISSSAASPL+NS  KTAVL
Sbjct: 430 IPPPCSDLSHPYSISSSAASPLLNSARKTAVL 461

BLAST of Chy6G112600 vs. NCBI nr
Match: XP_004134229.1 (probable polygalacturonase isoform X2 [Cucumis sativus] >XP_011651066.1 probable polygalacturonase isoform X2 [Cucumis sativus])

HSP 1 Score: 910 bits (2351), Expect = 0.0
Identity = 456/457 (99.78%), Postives = 456/457 (99.78%), Query Frame = 0

Query: 1   MRMLLVALLLFLQLSNAAKIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIA 60
           MRMLLVALLLFLQLSNAAKIIGEEL GQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIA
Sbjct: 1   MRMLLVALLLFLQLSNAAKIIGEELVGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIA 60

Query: 61  FQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVN 120
           FQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVN
Sbjct: 61  FQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVN 120

Query: 121 PLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHL 180
           PLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHL
Sbjct: 121 PLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHL 180

Query: 181 VEFEDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVC 240
           VEFEDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVC
Sbjct: 181 VEFEDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVC 240

Query: 241 IEGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISN 300
           IEGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISN
Sbjct: 241 IEGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISN 300

Query: 301 VLVEHVQLNNSFIGIQIRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDP 360
           VLVEHVQLNNSFIGIQIRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDP
Sbjct: 301 VLVEHVQLNNSFIGIQIRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDP 360

Query: 361 NALPIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSG 420
           NALPIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSG
Sbjct: 361 NALPIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSG 420

Query: 421 FSESVIPPPCSDLSTPYSISSSAASPLVNSTGKTAVL 457
           FSESVIPPPCSDLSTPYSISSSAASPLVNSTGKTAVL
Sbjct: 421 FSESVIPPPCSDLSTPYSISSSAASPLVNSTGKTAVL 457

BLAST of Chy6G112600 vs. NCBI nr
Match: XP_008438914.1 (PREDICTED: probable polygalacturonase isoform X2 [Cucumis melo])

HSP 1 Score: 905 bits (2339), Expect = 0.0
Identity = 453/457 (99.12%), Postives = 455/457 (99.56%), Query Frame = 0

Query: 1   MRMLLVALLLFLQLSNAAKIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIA 60
           MRMLLVALLLFLQLSNAA+IIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIA
Sbjct: 1   MRMLLVALLLFLQLSNAARIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIA 60

Query: 61  FQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVN 120
           FQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVN
Sbjct: 61  FQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVN 120

Query: 121 PLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHL 180
           PLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHL
Sbjct: 121 PLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHL 180

Query: 181 VEFEDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVC 240
           VEFEDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISV APSESPYTVGIVPDSSDHVC
Sbjct: 181 VEFEDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVYAPSESPYTVGIVPDSSDHVC 240

Query: 241 IEGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISN 300
           IEGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISN
Sbjct: 241 IEGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISN 300

Query: 301 VLVEHVQLNNSFIGIQIRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDP 360
           VLVEHVQLNNSFIGI+ RTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDP
Sbjct: 301 VLVEHVQLNNSFIGIEFRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDP 360

Query: 361 NALPIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSG 420
           NALPIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSG
Sbjct: 361 NALPIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSG 420

Query: 421 FSESVIPPPCSDLSTPYSISSSAASPLVNSTGKTAVL 457
           FSESVIPPPCSDLSTPYSISSSAASPLVNSTGKTAVL
Sbjct: 421 FSESVIPPPCSDLSTPYSISSSAASPLVNSTGKTAVL 457

BLAST of Chy6G112600 vs. NCBI nr
Match: XP_011651065.1 (probable polygalacturonase isoform X1 [Cucumis sativus] >KAE8650399.1 hypothetical protein Csa_009981 [Cucumis sativus])

HSP 1 Score: 902 bits (2332), Expect = 0.0
Identity = 451/452 (99.78%), Postives = 451/452 (99.78%), Query Frame = 0

Query: 6   VALLLFLQLSNAAKIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQNAI 65
           VALLLFLQLSNAAKIIGEEL GQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQNAI
Sbjct: 7   VALLLFLQLSNAAKIIGEELVGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQNAI 66

Query: 66  FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVNPLPSY 125
           FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVNPLPSY
Sbjct: 67  FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVNPLPSY 126

Query: 126 GRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHLVEFED 185
           GRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHLVEFED
Sbjct: 127 GRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHLVEFED 186

Query: 186 SQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEGCN 245
           SQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEGCN
Sbjct: 187 SQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEGCN 246

Query: 246 IATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLVEH 305
           IATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLVEH
Sbjct: 247 IATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLVEH 306

Query: 306 VQLNNSFIGIQIRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDPNALPI 365
           VQLNNSFIGIQIRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDPNALPI
Sbjct: 307 VQLNNSFIGIQIRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDPNALPI 366

Query: 366 VQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSESV 425
           VQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSESV
Sbjct: 367 VQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSESV 426

Query: 426 IPPPCSDLSTPYSISSSAASPLVNSTGKTAVL 457
           IPPPCSDLSTPYSISSSAASPLVNSTGKTAVL
Sbjct: 427 IPPPCSDLSTPYSISSSAASPLVNSTGKTAVL 458

BLAST of Chy6G112600 vs. NCBI nr
Match: XP_008438913.1 (PREDICTED: probable polygalacturonase isoform X1 [Cucumis melo])

HSP 1 Score: 898 bits (2320), Expect = 0.0
Identity = 448/452 (99.12%), Postives = 450/452 (99.56%), Query Frame = 0

Query: 6   VALLLFLQLSNAAKIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQNAI 65
           VALLLFLQLSNAA+IIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQNAI
Sbjct: 7   VALLLFLQLSNAARIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQNAI 66

Query: 66  FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVNPLPSY 125
           FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVNPLPSY
Sbjct: 67  FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVNPLPSY 126

Query: 126 GRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHLVEFED 185
           GRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHLVEFED
Sbjct: 127 GRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHLVEFED 186

Query: 186 SQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEGCN 245
           SQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISV APSESPYTVGIVPDSSDHVCIEGCN
Sbjct: 187 SQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVYAPSESPYTVGIVPDSSDHVCIEGCN 246

Query: 246 IATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLVEH 305
           IATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLVEH
Sbjct: 247 IATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLVEH 306

Query: 306 VQLNNSFIGIQIRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDPNALPI 365
           VQLNNSFIGI+ RTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDPNALPI
Sbjct: 307 VQLNNSFIGIEFRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDPNALPI 366

Query: 366 VQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSESV 425
           VQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSESV
Sbjct: 367 VQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSESV 426

Query: 426 IPPPCSDLSTPYSISSSAASPLVNSTGKTAVL 457
           IPPPCSDLSTPYSISSSAASPLVNSTGKTAVL
Sbjct: 427 IPPPCSDLSTPYSISSSAASPLVNSTGKTAVL 458

BLAST of Chy6G112600 vs. NCBI nr
Match: XP_038881575.1 (probable polygalacturonase isoform X4 [Benincasa hispida] >XP_038881581.1 probable polygalacturonase isoform X4 [Benincasa hispida])

HSP 1 Score: 893 bits (2307), Expect = 0.0
Identity = 449/457 (98.25%), Postives = 451/457 (98.69%), Query Frame = 0

Query: 1   MRMLLVALLLFLQLSNAAKIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIA 60
           MRML VALLL LQLSNAAKIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIA
Sbjct: 1   MRML-VALLLLLQLSNAAKIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIA 60

Query: 61  FQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVN 120
           FQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVN
Sbjct: 61  FQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVN 120

Query: 121 PLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHL 180
           PLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHL
Sbjct: 121 PLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHL 180

Query: 181 VEFEDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVC 240
           VEFEDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVC
Sbjct: 181 VEFEDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVC 240

Query: 241 IEGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISN 300
           IEGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISN
Sbjct: 241 IEGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISN 300

Query: 301 VLVEHVQLNNSFIGIQIRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDP 360
           VLVEHVQLNNSFIGIQ RT KGRGGYIKGIVVSDVEMENISTAFSASGHF SHPDDE+DP
Sbjct: 301 VLVEHVQLNNSFIGIQFRTAKGRGGYIKGIVVSDVEMENISTAFSASGHFESHPDDEFDP 360

Query: 361 NALPIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSG 420
           NALPIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSW+CSDVSG
Sbjct: 361 NALPIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWLCSDVSG 420

Query: 421 FSESVIPPPCSDLSTPYSISSSAASPLVNSTGKTAVL 457
           FSESVIPPPCSDLSTPYSISSSAASPLVNS GKTAVL
Sbjct: 421 FSESVIPPPCSDLSTPYSISSSAASPLVNSNGKTAVL 456

BLAST of Chy6G112600 vs. TAIR 10
Match: AT3G06770.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 571.2 bits (1471), Expect = 7.3e-163
Identity = 278/432 (64.35%), Postives = 344/432 (79.63%), Query Frame = 0

Query: 6   VALLLFLQLSNAAKIIGE-ELAGQ-CD--SKPTLDPRPHSVSILEFGAVGDGKTLNTIAF 65
           V  +L L L++   I      AG+ CD   + +L  RPHSVSI +FGAVGDGKTLNT+AF
Sbjct: 3   VVTILILALTSVIPIYANVHGAGKICDELGRRSLSTRPHSVSITDFGAVGDGKTLNTLAF 62

Query: 66  QNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVNP 125
           QNA+FYL SFADKGGAQLYVPPG WLTGS +LTSHLTLFLE GAVI+ SQDPSHWE+V+P
Sbjct: 63  QNAVFYLMSFADKGGAQLYVPPGHWLTGSFSLTSHLTLFLENGAVIVASQDPSHWEVVDP 122

Query: 126 LPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHLV 185
           LPSYGRGI++PGKRY+SLING  L DVV+TGD+G IDGQGLVWW+ F+SHSL Y+RPHL+
Sbjct: 123 LPSYGRGIDLPGKRYKSLINGNKLHDVVVTGDNGTIDGQGLVWWDRFTSHSLKYNRPHLI 182

Query: 186 EFEDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCI 245
           EF  S+ V+VSNLTFLN PAY+I+ +Y S+VY++ I   +  +SPYT+GIVPDSSD+VCI
Sbjct: 183 EFLSSENVIVSNLTFLNAPAYSIYSIYSSHVYIHKILAHSSPKSPYTIGIVPDSSDYVCI 242

Query: 246 EGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNV 305
           +   I  GYDAI+LKSGWD+YGIAY RP++N+HIR V+L+ +SGSSI+FGSEMSGGIS+V
Sbjct: 243 QNSTINVGYDAISLKSGWDEYGIAYSRPTENVHIRNVYLRGASGSSISFGSEMSGGISDV 302

Query: 306 LVEHVQLNNSFIGIQIRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDPN 365
           +V++  ++ S  GI  RTTKGRGGYIK I +S+++M  I TA  A+G FGSHPDD+YD N
Sbjct: 303 VVDNAHIHYSLTGIAFRTTKGRGGYIKEIDISNIDMLRIGTAIVANGSFGSHPDDKYDVN 362

Query: 366 ALPIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGF 425
           ALP+V  I L N+ G NI IAG   GI+ESPF+S+ LSN++ S++S SS SW CS V G 
Sbjct: 363 ALPLVSHIRLSNISGENIGIAGKLFGIKESPFSSVTLSNVSLSMSSGSSVSWQCSYVYGS 422

Query: 426 SESVIPPPCSDL 434
           SESVIP PC +L
Sbjct: 423 SESVIPEPCPEL 434

BLAST of Chy6G112600 vs. TAIR 10
Match: AT3G16850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 565.5 bits (1456), Expect = 4.0e-161
Identity = 273/439 (62.19%), Postives = 340/439 (77.45%), Query Frame = 0

Query: 1   MRMLLVALLLFLQLSNAAKIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIA 60
           M + LV LL F  L     I GE     C     LDPRPHSVSILEFGAVGDGKTLNTIA
Sbjct: 3   MPVALVWLLAFTIL----LISGEGNNAICKENFKLDPRPHSVSILEFGAVGDGKTLNTIA 62

Query: 61  FQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVN 120
           FQNA+FYLKSFADKGGAQLYVPPGKWLTGS NLTSHLTLFLEKGA IL S DPSHW++V+
Sbjct: 63  FQNAVFYLKSFADKGGAQLYVPPGKWLTGSFNLTSHLTLFLEKGATILASPDPSHWDVVS 122

Query: 121 PLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHL 180
           PLPSYGRGIE+PGKRYRSLING NL DVVITG++G  DGQG  WW W  S SLNYSRPH+
Sbjct: 123 PLPSYGRGIELPGKRYRSLINGDNLIDVVITGENGTFDGQGAAWWEWLESGSLNYSRPHI 182

Query: 181 VEFEDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVC 240
           +EF  S+++++SNLTFLN PA NIHPVYCS +++  + +    +SP+ +G+ PDSSD+VC
Sbjct: 183 IEFVSSKHILISNLTFLNAPAINIHPVYCSQIHIRKVLIETSVDSPHVLGVAPDSSDNVC 242

Query: 241 IEGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISN 300
           IE   I  G+DA++LKSGWDQYGI YGRP+  +HIR + L+S +G+ I+FGSEMSGG+S+
Sbjct: 243 IEDSTINVGHDAVSLKSGWDQYGIHYGRPTTAVHIRNLRLKSPTGAGISFGSEMSGGVSD 302

Query: 301 VLVEHVQLNNSFIGIQIRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDP 360
           V VE + +++S IG+  RTT+GRGGYI+ I +SDV++ ++ TA  A+GH GSHPDD++D 
Sbjct: 303 VTVERLNIHSSLIGVAFRTTRGRGGYIRNITISDVDLTSVDTAIVANGHTGSHPDDKFDR 362

Query: 361 NALPIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSG 420
           +ALP+V  I ++N  G +I +AGN +GI ESPFTSI L++I   + + S  SWICS+VSG
Sbjct: 363 DALPVVTHIVMRNFTGVDIGVAGNLTGIGESPFTSICLADI--HLQTRSEESWICSNVSG 422

Query: 421 FSESVIPPPCSDL-STPYS 439
           FS+ V P PC +L S+P S
Sbjct: 423 FSDDVSPEPCQELMSSPSS 435

BLAST of Chy6G112600 vs. TAIR 10
Match: AT5G49215.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 552.4 bits (1422), Expect = 3.5e-157
Identity = 272/434 (62.67%), Postives = 333/434 (76.73%), Query Frame = 0

Query: 6   VALLLFLQLSNAAKIIGEE--LAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQN 65
           VAL+L L LS+   I G    +   CD       RPHSV I +FGAVGDGKTLNT+AFQN
Sbjct: 5   VALVLLLTLSSVILINGRSYGVGNICDRGRRPSERPHSVKITDFGAVGDGKTLNTLAFQN 64

Query: 66  AIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVNPLP 125
           A+FYLKSFADKGGAQLYVPPG+WLTGS NLTSHLTLFLEK AVIL SQDPSHW++ + LP
Sbjct: 65  AVFYLKSFADKGGAQLYVPPGRWLTGSFNLTSHLTLFLEKDAVILASQDPSHWQVTDALP 124

Query: 126 SYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHLVEF 185
           SYGRGI++PGKRY SLING  L DVV+TGD+G IDGQGLVWW+ F+SHSL YSRPHLVEF
Sbjct: 125 SYGRGIDLPGKRYMSLINGDMLHDVVVTGDNGTIDGQGLVWWDRFNSHSLEYSRPHLVEF 184

Query: 186 EDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEG 245
             ++ V+VSNLTFLN PAY IH VYC N+Y++ ++ +   ESPYT+GIVPDSS++VCI+ 
Sbjct: 185 VSAENVIVSNLTFLNAPAYTIHSVYCRNLYIHRVTANTCPESPYTIGIVPDSSENVCIQE 244

Query: 246 CNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLV 305
            +I  GYDAI+LKSG D+YG++Y RP+ N+ IR V+L+++SGSSI+FGSEMSGGIS+V V
Sbjct: 245 SSINMGYDAISLKSGCDEYGLSYARPTANVQIRNVYLRAASGSSISFGSEMSGGISDVEV 304

Query: 306 EHVQLNNSFIGIQIRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDPNAL 365
               ++NS  GI  RTT GRGGYIK I +S++ M N+ TAF A+G FG+HPD  +D NA 
Sbjct: 305 SDAHIHNSLSGIAFRTTNGRGGYIKEIDISNIHMVNVGTAFLANGSFGTHPDSGFDENAY 364

Query: 366 PIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSS--TSWICSDVSGF 425
           P+V  I L ++ G NI  AG F G +ESPFTSI LSNI+ SI +S+S   SW CS V G 
Sbjct: 365 PLVSHIRLHDIVGENISTAGYFFGTKESPFTSILLSNISLSIKNSASPADSWQCSYVDGS 424

Query: 426 SESVIPPPCSDLST 436
           SE V+P PC +L +
Sbjct: 425 SEFVVPEPCLELKS 438

BLAST of Chy6G112600 vs. TAIR 10
Match: AT5G49215.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 552.4 bits (1422), Expect = 3.5e-157
Identity = 272/434 (62.67%), Postives = 333/434 (76.73%), Query Frame = 0

Query: 6   VALLLFLQLSNAAKIIGEE--LAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQN 65
           VAL+L L LS+   I G    +   CD       RPHSV I +FGAVGDGKTLNT+AFQN
Sbjct: 3   VALVLLLTLSSVILINGRSYGVGNICDRGRRPSERPHSVKITDFGAVGDGKTLNTLAFQN 62

Query: 66  AIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVNPLP 125
           A+FYLKSFADKGGAQLYVPPG+WLTGS NLTSHLTLFLEK AVIL SQDPSHW++ + LP
Sbjct: 63  AVFYLKSFADKGGAQLYVPPGRWLTGSFNLTSHLTLFLEKDAVILASQDPSHWQVTDALP 122

Query: 126 SYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHLVEF 185
           SYGRGI++PGKRY SLING  L DVV+TGD+G IDGQGLVWW+ F+SHSL YSRPHLVEF
Sbjct: 123 SYGRGIDLPGKRYMSLINGDMLHDVVVTGDNGTIDGQGLVWWDRFNSHSLEYSRPHLVEF 182

Query: 186 EDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEG 245
             ++ V+VSNLTFLN PAY IH VYC N+Y++ ++ +   ESPYT+GIVPDSS++VCI+ 
Sbjct: 183 VSAENVIVSNLTFLNAPAYTIHSVYCRNLYIHRVTANTCPESPYTIGIVPDSSENVCIQE 242

Query: 246 CNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLV 305
            +I  GYDAI+LKSG D+YG++Y RP+ N+ IR V+L+++SGSSI+FGSEMSGGIS+V V
Sbjct: 243 SSINMGYDAISLKSGCDEYGLSYARPTANVQIRNVYLRAASGSSISFGSEMSGGISDVEV 302

Query: 306 EHVQLNNSFIGIQIRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDPNAL 365
               ++NS  GI  RTT GRGGYIK I +S++ M N+ TAF A+G FG+HPD  +D NA 
Sbjct: 303 SDAHIHNSLSGIAFRTTNGRGGYIKEIDISNIHMVNVGTAFLANGSFGTHPDSGFDENAY 362

Query: 366 PIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSS--TSWICSDVSGF 425
           P+V  I L ++ G NI  AG F G +ESPFTSI LSNI+ SI +S+S   SW CS V G 
Sbjct: 363 PLVSHIRLHDIVGENISTAGYFFGTKESPFTSILLSNISLSIKNSASPADSWQCSYVDGS 422

Query: 426 SESVIPPPCSDLST 436
           SE V+P PC +L +
Sbjct: 423 SEFVVPEPCLELKS 436

BLAST of Chy6G112600 vs. TAIR 10
Match: AT3G06770.3 (Pectin lyase-like superfamily protein )

HSP 1 Score: 511.1 bits (1315), Expect = 8.9e-145
Identity = 241/364 (66.21%), Postives = 298/364 (81.87%), Query Frame = 0

Query: 70  SFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVNPLPSYGRGI 129
           SFADKGGAQLYVPPG WLTGS +LTSHLTLFLE GAVI+ SQDPSHWE+V+PLPSYGRGI
Sbjct: 2   SFADKGGAQLYVPPGHWLTGSFSLTSHLTLFLENGAVIVASQDPSHWEVVDPLPSYGRGI 61

Query: 130 EVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHLVEFEDSQYV 189
           ++PGKRY+SLING  L DVV+TGD+G IDGQGLVWW+ F+SHSL Y+RPHL+EF  S+ V
Sbjct: 62  DLPGKRYKSLINGNKLHDVVVTGDNGTIDGQGLVWWDRFTSHSLKYNRPHLIEFLSSENV 121

Query: 190 VVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEGCNIATG 249
           +VSNLTFLN PAY+I+ +Y S+VY++ I   +  +SPYT+GIVPDSSD+VCI+   I  G
Sbjct: 122 IVSNLTFLNAPAYSIYSIYSSHVYIHKILAHSSPKSPYTIGIVPDSSDYVCIQNSTINVG 181

Query: 250 YDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLVEHVQLN 309
           YDAI+LKSGWD+YGIAY RP++N+HIR V+L+ +SGSSI+FGSEMSGGIS+V+V++  ++
Sbjct: 182 YDAISLKSGWDEYGIAYSRPTENVHIRNVYLRGASGSSISFGSEMSGGISDVVVDNAHIH 241

Query: 310 NSFIGIQIRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDPNALPIVQDI 369
            S  GI  RTTKGRGGYIK I +S+++M  I TA  A+G FGSHPDD+YD NALP+V  I
Sbjct: 242 YSLTGIAFRTTKGRGGYIKEIDISNIDMLRIGTAIVANGSFGSHPDDKYDVNALPLVSHI 301

Query: 370 TLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSESVIPPP 429
            L N+ G NI IAG   GI+ESPF+S+ LSN++ S++S SS SW CS V G SESVIP P
Sbjct: 302 RLSNISGENIGIAGKLFGIKESPFSSVTLSNVSLSMSSGSSVSWQCSYVYGSSESVIPEP 361

Query: 430 CSDL 434
           C +L
Sbjct: 362 CPEL 365

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A7PZL31.0e-11348.26Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 ... [more]
Q9LW071.8e-2527.76Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g1572... [more]
Q8RY292.4e-2526.73Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2[more]
P432121.0e-2326.11Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1[more]
P353362.1e-2123.82Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LAN43.7e-26399.78Pectate_lyase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G16... [more]
A0A1S3AXL39.0e-26299.12probable polygalacturonase isoform X2 OS=Cucumis melo OX=3656 GN=LOC103483871 PE... [more]
A0A1S3AX491.1e-25999.12probable polygalacturonase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483871 PE... [more]
A0A6J1GUW57.0e-24693.23probable polygalacturonase isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111457... [more]
A0A6J1GW417.7e-24593.81probable polygalacturonase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111457... [more]
Match NameE-valueIdentityDescription
XP_004134229.10.099.78probable polygalacturonase isoform X2 [Cucumis sativus] >XP_011651066.1 probable... [more]
XP_008438914.10.099.12PREDICTED: probable polygalacturonase isoform X2 [Cucumis melo][more]
XP_011651065.10.099.78probable polygalacturonase isoform X1 [Cucumis sativus] >KAE8650399.1 hypothetic... [more]
XP_008438913.10.099.12PREDICTED: probable polygalacturonase isoform X1 [Cucumis melo][more]
XP_038881575.10.098.25probable polygalacturonase isoform X4 [Benincasa hispida] >XP_038881581.1 probab... [more]
Match NameE-valueIdentityDescription
AT3G06770.27.3e-16364.35Pectin lyase-like superfamily protein [more]
AT3G16850.14.0e-16162.19Pectin lyase-like superfamily protein [more]
AT5G49215.13.5e-15762.67Pectin lyase-like superfamily protein [more]
AT5G49215.23.5e-15762.67Pectin lyase-like superfamily protein [more]
AT3G06770.38.9e-14566.21Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 236..257
e-value: 930.0
score: 7.4
coord: 327..348
e-value: 6800.0
score: 1.3
coord: 361..383
e-value: 4700.0
score: 2.4
coord: 186..208
e-value: 1700.0
score: 5.6
coord: 270..292
e-value: 780.0
score: 8.0
coord: 209..235
e-value: 5100.0
score: 2.2
coord: 298..319
e-value: 1300.0
score: 6.4
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 26..422
e-value: 5.1E-112
score: 376.9
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 134..419
e-value: 3.3E-34
score: 118.4
IPR024535Pectate lyase superfamily proteinPFAMPF12708Pectate_lyase_3coord: 42..99
e-value: 1.3E-7
score: 31.6
NoneNo IPR availablePANTHERPTHR31339PECTIN LYASE-RELATEDcoord: 5..447
NoneNo IPR availablePANTHERPTHR31339:SF15PECTIN LYASE-LIKE SUPERFAMILY PROTEINcoord: 5..447
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 37..435

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy6G112600.1Chy6G112600.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity