Chy6G112130 (gene) Cucumber (hystrix) v1

Overview
NameChy6G112130
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
LocationchrH06: 4684088 .. 4685423 (+)
RNA-Seq ExpressionChy6G112130
SyntenyChy6G112130
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCCAGTACCTATTTCTCCAATCAAGGTAGGGCACATTGATGATGTGCAAGAACTCAGAAAGTTTAAGCCTAACATTATTCCCGAGAGATTTATCAGGGACATACAAGAGAGACCAACACCAGCAACACCTTTAATCTCATCCTCTGACTTTCCCACTATTGATTTATCCAAGCTTCTAAAGGGAAACAGAGATGAAATTCTGCAGCTAGCCACTGCCTGCGAGGAATGGGGATTTTTTCAGGTAAAAACTGATTGTTTATTTCCTTTTCAGCAGCTTAAGGTAGGCTGTCAAGACTAATGCCTTGGTTATCTATTAGGTAATCAATCACGGAATCGCTCTGAACCTACTAGAAAATATAGAGGGACGGGCAATGGAATTCTTCAGGCTACCTTTGGAAGAGAAACAAAAGTACGCAATGGTACCAGGGACAGTTCAAGGATATGGGCAAGCATTTGTGTTCTCAGAGCACCAAAAACTGGATTGGTGTAACATGTTCGCTCTAGGAGTTATACCCGACTTTATAAGGAACCCTCTATTCTGGCCAAAGAAGCCAGCTAACTTCAGGTGGTTGTTCGTGTTTTTCTTTTTCCAATACTGTCACCATTGCATTATCCATCCAGTAGCTTACAAGTATACTCCTTTTCTTTTGCAGCAATACTGTGGAGACTTACTCAAAAGAAGTAAGGAAGCTTTGCAAGAATCTATTGAAGTACATAGCTTTAGGCCTTGGTCTAAAAGAGGACCTATTTGAAGAAGCGTTTGGGGTGGCCGTGCAGGCAATAAGGATGAATTACTATCCACCGTGTTCGAGACCTGATCTTGTTTTGGGGTTGAGTCCACACTCCGATGGAAGTGCTTTAACTGTTTTGCAGCAAGGAAAGGGAAGCTCAGTAGGGCTCCAGATCCTTAAAGATGACAAGTGGGTTCCTGTTCAACCCATCCCAAATGCACTTGTGATCAACATTGGCGATACAATGGAGGTACAAATGAAACTTTGTCTAATTCTAGAAATGGTTATCTATCGCATAATGGAAACTTAACCTCCTTTCTCATTGTTACATGTATGCAGGTTGTTACAAATGGCAGGTACAAATCCGTCGAGCACAGAGCTGTGACTCACAAACACACTGACCGACTTTCCCTCGTCACATTTTATGCTCCTAGCTATGACATAGAGCTTGGTCCAATGCCAGAATTCGTGGACAAAAACAATCCATGCAAGTATAGAAGATACAATCATGGAGAATACAGTAAGCACTATGTAACGAACAAGTTACAGGGAAAGAGAACCCTGGAGTTTGCCAAGATTCCAATAAAAAACTCCTGCTAA

mRNA sequence

ATGGCTCCAGTACCTATTTCTCCAATCAAGGTAGGGCACATTGATGATGTGCAAGAACTCAGAAAGTTTAAGCCTAACATTATTCCCGAGAGATTTATCAGGGACATACAAGAGAGACCAACACCAGCAACACCTTTAATCTCATCCTCTGACTTTCCCACTATTGATTTATCCAAGCTTCTAAAGGGAAACAGAGATGAAATTCTGCAGCTAGCCACTGCCTGCGAGGAATGGGGATTTTTTCAGGTAATCAATCACGGAATCGCTCTGAACCTACTAGAAAATATAGAGGGACGGGCAATGGAATTCTTCAGGCTACCTTTGGAAGAGAAACAAAAGTACGCAATGGTACCAGGGACAGTTCAAGGATATGGGCAAGCATTTGTGTTCTCAGAGCACCAAAAACTGGATTGGTGTAACATGTTCGCTCTAGGAGTTATACCCGACTTTATAAGGAACCCTCTATTCTGGCCAAAGAAGCCAGCTAACTTCAGCAATACTGTGGAGACTTACTCAAAAGAAGTAAGGAAGCTTTGCAAGAATCTATTGAAGTACATAGCTTTAGGCCTTGGTCTAAAAGAGGACCTATTTGAAGAAGCGTTTGGGGTGGCCGTGCAGGCAATAAGGATGAATTACTATCCACCGTGTTCGAGACCTGATCTTGTTTTGGGGTTGAGTCCACACTCCGATGGAAGTGCTTTAACTGTTTTGCAGCAAGGAAAGGGAAGCTCAGTAGGGCTCCAGATCCTTAAAGATGACAAGTGGGTTCCTGTTCAACCCATCCCAAATGCACTTGTGATCAACATTGGCGATACAATGGAGGTTGTTACAAATGGCAGGTACAAATCCGTCGAGCACAGAGCTGTGACTCACAAACACACTGACCGACTTTCCCTCGTCACATTTTATGCTCCTAGCTATGACATAGAGCTTGGTCCAATGCCAGAATTCGTGGACAAAAACAATCCATGCAAGTATAGAAGATACAATCATGGAGAATACAGTAAGCACTATGTAACGAACAAGTTACAGGGAAAGAGAACCCTGGAGTTTGCCAAGATTCCAATAAAAAACTCCTGCTAA

Coding sequence (CDS)

ATGGCTCCAGTACCTATTTCTCCAATCAAGGTAGGGCACATTGATGATGTGCAAGAACTCAGAAAGTTTAAGCCTAACATTATTCCCGAGAGATTTATCAGGGACATACAAGAGAGACCAACACCAGCAACACCTTTAATCTCATCCTCTGACTTTCCCACTATTGATTTATCCAAGCTTCTAAAGGGAAACAGAGATGAAATTCTGCAGCTAGCCACTGCCTGCGAGGAATGGGGATTTTTTCAGGTAATCAATCACGGAATCGCTCTGAACCTACTAGAAAATATAGAGGGACGGGCAATGGAATTCTTCAGGCTACCTTTGGAAGAGAAACAAAAGTACGCAATGGTACCAGGGACAGTTCAAGGATATGGGCAAGCATTTGTGTTCTCAGAGCACCAAAAACTGGATTGGTGTAACATGTTCGCTCTAGGAGTTATACCCGACTTTATAAGGAACCCTCTATTCTGGCCAAAGAAGCCAGCTAACTTCAGCAATACTGTGGAGACTTACTCAAAAGAAGTAAGGAAGCTTTGCAAGAATCTATTGAAGTACATAGCTTTAGGCCTTGGTCTAAAAGAGGACCTATTTGAAGAAGCGTTTGGGGTGGCCGTGCAGGCAATAAGGATGAATTACTATCCACCGTGTTCGAGACCTGATCTTGTTTTGGGGTTGAGTCCACACTCCGATGGAAGTGCTTTAACTGTTTTGCAGCAAGGAAAGGGAAGCTCAGTAGGGCTCCAGATCCTTAAAGATGACAAGTGGGTTCCTGTTCAACCCATCCCAAATGCACTTGTGATCAACATTGGCGATACAATGGAGGTTGTTACAAATGGCAGGTACAAATCCGTCGAGCACAGAGCTGTGACTCACAAACACACTGACCGACTTTCCCTCGTCACATTTTATGCTCCTAGCTATGACATAGAGCTTGGTCCAATGCCAGAATTCGTGGACAAAAACAATCCATGCAAGTATAGAAGATACAATCATGGAGAATACAGTAAGCACTATGTAACGAACAAGTTACAGGGAAAGAGAACCCTGGAGTTTGCCAAGATTCCAATAAAAAACTCCTGCTAA

Protein sequence

MAPVPISPIKVGHIDDVQELRKFKPNIIPERFIRDIQERPTPATPLISSSDFPTIDLSKLLKGNRDEILQLATACEEWGFFQVINHGIALNLLENIEGRAMEFFRLPLEEKQKYAMVPGTVQGYGQAFVFSEHQKLDWCNMFALGVIPDFIRNPLFWPKKPANFSNTVETYSKEVRKLCKNLLKYIALGLGLKEDLFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQGKGSSVGLQILKDDKWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKHTDRLSLVTFYAPSYDIELGPMPEFVDKNNPCKYRRYNHGEYSKHYVTNKLQGKRTLEFAKIPIKNSC*
Homology
BLAST of Chy6G112130 vs. ExPASy Swiss-Prot
Match: Q39224 (Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 4.0e-76
Identity = 142/346 (41.04%), Postives = 210/346 (60.69%), Query Frame = 0

Query: 14  IDDVQELRKFKP-NIIPERFIRDIQERPTPATPLISSSDFPTIDLSKLLKGNR--DEILQ 73
           +  VQE+ K K    +P R++R  Q++           + P ID+ +L        E+ +
Sbjct: 14  VPSVQEMVKEKTITTVPPRYVRSDQDKTEVDDDFDVKIEIPIIDMKRLCSSTTMDSEVEK 73

Query: 74  LATACEEWGFFQVINHGIALNLLENIEGRAMEFFRLPLEEKQKYAMVPGTVQGYGQAFVF 133
           L  AC+EWGFFQ++NHGI  + L+ ++    +FF LP+EEK+K+   P  ++G+GQAFV 
Sbjct: 74  LDFACKEWGFFQLVNHGIDSSFLDKVKSEIQDFFNLPMEEKKKFWQRPDEIEGFGQAFVV 133

Query: 134 SEHQKLDWCNMFALGVIPDFIRNPLFWPKKPANFSNTVETYSKEVRKLCKNLLKYIALGL 193
           SE QKLDW ++F   V P  +R P  +PK P  F +T+E YS EV+ + K L+  +A  L
Sbjct: 134 SEDQKLDWADLFFHTVQPVELRKPHLFPKLPLPFRDTLEMYSSEVQSVAKILIAKMARAL 193

Query: 194 GLKEDLFEEAFG--VAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQGKGSSVGLQ 253
            +K +  E+ F    +VQ++RMNYYPPC +PD V+GL+PHSD   LTVL Q      GLQ
Sbjct: 194 EIKPEELEKLFDDVDSVQSMRMNYYPPCPQPDQVIGLTPHSDSVGLTVLMQ-VNDVEGLQ 253

Query: 254 ILKDDKWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKHTDRLSLVTFYAPSYD 313
           I KD KWVPV+P+PNA ++NIGD +E++TNG Y+S+EHR V +   +RLS+ TF+     
Sbjct: 254 IKKDGKWVPVKPLPNAFIVNIGDVLEIITNGTYRSIEHRGVVNSEKERLSIATFHNVGMY 313

Query: 314 IELGPMPEFVDKNNPCKYRRYNHGEYSKHYVTNKLQGKRTLEFAKI 355
            E+GP    V++    +++R    EY+    +  L GK  L+  +I
Sbjct: 314 KEVGPAKSLVERQKVARFKRLTMKEYNDGLFSRTLDGKAYLDALRI 358

BLAST of Chy6G112130 vs. ExPASy Swiss-Prot
Match: Q94LP4 (2-oxoglutarate-dependent dioxygenase 11 OS=Oryza sativa subsp. japonica OX=39947 GN=2ODD11 PE=1 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 4.5e-72
Identity = 134/351 (38.18%), Postives = 210/351 (59.83%), Query Frame = 0

Query: 6   ISPIKVGHIDDVQELRKFKPNIIPERFIRDIQERPTPATPLISSSDFPTIDLSKLL--KG 65
           +  + V ++  + E+       IPER+IR                  P IDL KLL  + 
Sbjct: 7   VGSLPVPNVQALAEICNDPDEHIPERYIRPEASSEEVINNYQGDMAIPIIDLKKLLCPQS 66

Query: 66  NRDEILQLATACEEWGFFQVINHGIALNLLENIEGRAMEFFRLPLEEKQKYAMVPGTVQG 125
           + +E ++L +AC+ WGFF +INHG+   ++ N++   ++FF  PL+ K++Y  +P +++G
Sbjct: 67  SEEECVKLRSACQYWGFFLLINHGVPDEVIANLKRDIVDFFSQPLDTKKEYTQLPNSLEG 126

Query: 126 YGQAFVFSEHQKLDWCNMFALGVIPDFIRNPLFWPKKPANFSNTVETYSKEVRKLCKNLL 185
           YGQ+FVFSE QKLDW +M  L V P   R+  FWP  PA+F  +++ YS E + L   L 
Sbjct: 127 YGQSFVFSEDQKLDWADMLYLHVHPSDSRDLRFWPTSPASFRQSIDAYSSETKSLALCLF 186

Query: 186 KYIALGLGLKEDLFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQGKGS 245
           +++A  +G K +   + F    + +RM YYPPC + D V+GLSPHSD   LT+L +   +
Sbjct: 187 EFMAKAVGAKPESLLDLFEEQPRGLRMAYYPPCRQADKVMGLSPHSDAGGLTLLLE-INN 246

Query: 246 SVGLQILKDDKWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKHTDRLSLVTFY 305
             GLQI KD KW  +     AL+ NIGDT+E+++NG+++SVEHRAV + + +R+S   F+
Sbjct: 247 VQGLQIKKDGKWFSIDAPNGALIANIGDTLEILSNGKFRSVEHRAVINPNKERISAALFH 306

Query: 306 APSYDIELGPMPEFVDKNNPCKYRRYNHGEYSKHYVTNKLQGKRTLEFAKI 355
            PS ++ + P+PEFV K+   KYR  ++ ++ K   T +L GK  +E  K+
Sbjct: 307 YPSENMVISPLPEFV-KDGKVKYRSISYLDFMKQIFTQQLDGKNRVEVLKL 355

BLAST of Chy6G112130 vs. ExPASy Swiss-Prot
Match: D4N501 (Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Papaver somniferum OX=3469 GN=DIOX2 PE=2 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 1.9e-70
Identity = 135/347 (38.90%), Postives = 205/347 (59.08%), Query Frame = 0

Query: 14  IDDVQELRKFKPNIIPERFIRDIQ--ERPTPATPLISSSDFPTIDLSKLLKG----NRDE 73
           I  VQEL K     IP R+I  ++  + P  A+ +      P ID+  L+       + E
Sbjct: 15  IPSVQELAKLTLAEIPSRYICTVENLQLPVGASVIDDHETVPVIDIENLISSEPVTEKLE 74

Query: 74  ILQLATACEEWGFFQVINHGIALNLLENIEGRAMEFFRLPLEEKQKYAMVPGTVQGYGQA 133
           + +L +AC+EWGFFQV+NHG+  +L++N++     FF L + EK KY    G V+G+GQA
Sbjct: 75  LDRLHSACKEWGFFQVVNHGVDTSLVDNVKSDIQGFFNLSMNEKIKYGQKDGDVEGFGQA 134

Query: 134 FVFSEHQKLDWCNMFALGVIPDFIRNPLFWPKKPANFSNTVETYSKEVRKLCKNLLKYIA 193
           FV SE Q LDW ++F +  +P  +R P  + K P     T+E+YS E++KL   L + + 
Sbjct: 135 FVASEDQTLDWADIFMILTLPLHLRKPHLFSKLPLPLRETIESYSSEMKKLSMVLFEKME 194

Query: 194 LGL---GLKEDLFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQGKGSS 253
             L    ++     E F    Q +RMNYYPPC +P+L +GL+PHSD   LT+L Q     
Sbjct: 195 KALQVQAVEIKEISEVFKDMTQVMRMNYYPPCPQPELAIGLTPHSDFGGLTILLQ-LNEV 254

Query: 254 VGLQILKDDKWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKHTDRLSLVTFYA 313
            GLQI  + +W+ V+P+PNA V+N+GD +E++TNG Y+SV+HRAV +   +RLS+ TF+ 
Sbjct: 255 EGLQIKNEGRWISVKPLPNAFVVNVGDVLEIMTNGMYRSVDHRAVVNSTKERLSIATFHD 314

Query: 314 PSYDIELGPMPEFVDKNNPCKYRR-YNHGEYSKHYVTNKLQGKRTLE 351
           P+ + E+GP+   +  N P  +R    +GE  + + + KL GK  L+
Sbjct: 315 PNLESEIGPISSLITPNTPALFRSGSTYGELVEEFHSRKLDGKSFLD 360

BLAST of Chy6G112130 vs. ExPASy Swiss-Prot
Match: O80449 (Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 9.5e-70
Identity = 128/325 (39.38%), Postives = 192/325 (59.08%), Query Frame = 0

Query: 14  IDDVQELRKFKPNIIPERFIRDIQERPT-PATPLISSSDFPTIDLSKLLKGNRDEILQLA 73
           I  VQ L +     +P R+++   +RP    T   +  + P +D++ +  G  + +  + 
Sbjct: 9   IVSVQSLSQTGVPTVPNRYVKPAHQRPVFNTTQSDAGIEIPVLDMNDVW-GKPEGLRLVR 68

Query: 74  TACEEWGFFQVINHGIALNLLENIEGRAMEFFRLPLEEKQKYAMVPGTVQGYGQAFVFSE 133
           +ACEEWGFFQ++NHG+  +L+E + G   EFF LPLEEK+KYA  P T +GYG      +
Sbjct: 69  SACEEWGFFQMVNHGVTHSLMERVRGAWREFFELPLEEKRKYANSPDTYEGYGSRLGVVK 128

Query: 134 HQKLDWCNMFALGVIPDFIRNPLFWPKKPANFSNTVETYSKEVRKLCKNLLKYIALGLGL 193
             KLDW + F L  +P  IRNP  WP +P      +E Y +EVRKLC+ L + ++  LGL
Sbjct: 129 DAKLDWSDYFFLNYLPSSIRNPSKWPSQPPKIRELIEKYGEEVRKLCERLTETLSESLGL 188

Query: 194 KEDLFEEAFGVAVQ---AIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQGKGSSVGLQI 253
           K +   +A G   +   ++R N+YP C +P L LGLS HSD   +T+L   +    GLQ+
Sbjct: 189 KPNKLMQALGGGDKVGASLRTNFYPKCPQPQLTLGLSSHSDPGGITILLPDE-KVAGLQV 248

Query: 254 LKDDKWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKHTDRLSLVTFYAPSYDI 313
            + D WV ++ +PNAL++NIGD +++++NG YKSVEH+ + +   +R+SL  FY P  DI
Sbjct: 249 RRGDGWVTIKSVPNALIVNIGDQLQILSNGIYKSVEHQVIVNSGMERVSLAFFYNPRSDI 308

Query: 314 ELGPMPEFVDKNNPCKYRRYNHGEY 335
            +GP+ E V  N P  Y+     EY
Sbjct: 309 PVGPIEELVTANRPALYKPIRFDEY 331

BLAST of Chy6G112130 vs. ExPASy Swiss-Prot
Match: D4N502 (Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 4.7e-69
Identity = 138/360 (38.33%), Postives = 212/360 (58.89%), Query Frame = 0

Query: 9   IKVGH---IDDVQELRKFKPNIIPERFIRDIQERP--TPATPLISSSDFPTIDLSKLLKG 68
           IK+G+   I  VQEL K     IP R+     E P       +      P IDL  LL  
Sbjct: 7   IKLGNGLSIPSVQELAKLTLAEIPSRY-TCTGESPLNNIGASVTDDETVPVIDLQNLLSP 66

Query: 69  ----NRDEILQLATACEEWGFFQVINHGIALNLLENIEGRAMEFFRLPLEEKQKYAMVPG 128
                + E+ +L +AC+EWGFFQ++NHG+   L++NI+     FF LP+ EK KY    G
Sbjct: 67  EPVVGKLELDKLHSACKEWGFFQLVNHGVDALLMDNIKSEIKGFFNLPMNEKTKYGQQDG 126

Query: 129 TVQGYGQAFVFSEHQKLDWCNMFALGVIPDFIRNPLFWPKKPANFSNTVETYSKEVRKLC 188
             +G+GQ ++ SE Q+LDW  +F++  +P  +R P  +P+ P  F  T+E+Y  +++KL 
Sbjct: 127 DFEGFGQPYIESEDQRLDWTEVFSMLSLPLHLRKPHLFPELPLPFRETLESYLSKMKKLS 186

Query: 189 KNLLKYIALGLGLKE-----DLFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSAL 248
             + + +   L L E     DLFE+     +Q +RMNYYPPC RP+LVLGL+ HSD S L
Sbjct: 187 TVVFEMLEKSLQLVEIKGMTDLFED----GLQTMRMNYYPPCPRPELVLGLTSHSDFSGL 246

Query: 249 TVLQQGKGSSVGLQILKDDKWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKHT 308
           T+L Q      GLQI K+++W+ ++P+P+A ++N+GD +E++TNG Y+SVEHRAV +   
Sbjct: 247 TILLQ-LNEVEGLQIRKEERWISIKPLPDAFIVNVGDILEIMTNGIYRSVEHRAVVNSTK 306

Query: 309 DRLSLVTFYAPSYDIELGPMPEFVDKNNPCKYRRYNHGEYSKHYVTNKLQGKRTLEFAKI 355
           +RLS+ TF+    + E+GP+   V    P  ++R  + +  K  ++ KL GK  L++ ++
Sbjct: 307 ERLSIATFHDSKLESEIGPISSLVTPETPALFKRGRYEDILKENLSRKLDGKSFLDYMRM 360

BLAST of Chy6G112130 vs. ExPASy TrEMBL
Match: A0A5D3CXG4 (Protein SRG1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G00820 PE=3 SV=1)

HSP 1 Score: 733.0 bits (1891), Expect = 5.9e-208
Identity = 350/360 (97.22%), Postives = 355/360 (98.61%), Query Frame = 0

Query: 1   MAPVPISPIKVGHIDDVQELRKFKPNIIPERFIRDIQERPTPATPLISSSDFPTIDLSKL 60
           MAPVPISPIKVGHIDDVQELRKFKPNIIPERFIRDIQERPTPATPLISSSD PTIDLSKL
Sbjct: 1   MAPVPISPIKVGHIDDVQELRKFKPNIIPERFIRDIQERPTPATPLISSSDIPTIDLSKL 60

Query: 61  LKGNRDEILQLATACEEWGFFQVINHGIALNLLENIEGRAMEFFRLPLEEKQKYAMVPGT 120
           LKGN+DEILQL TACEEWGFFQVINHGIALNLLENIEG+AMEFFRLPLEEKQKYAMVPGT
Sbjct: 61  LKGNKDEILQLTTACEEWGFFQVINHGIALNLLENIEGQAMEFFRLPLEEKQKYAMVPGT 120

Query: 121 VQGYGQAFVFSEHQKLDWCNMFALGVIPDFIRNPLFWPKKPANFSNTVETYSKEVRKLCK 180
           VQGYGQAFVFSEHQKLDWCNMFALGVIPDF+RNPLFWPKKPANFSNTVE YSKEVRKLCK
Sbjct: 121 VQGYGQAFVFSEHQKLDWCNMFALGVIPDFLRNPLFWPKKPANFSNTVEIYSKEVRKLCK 180

Query: 181 NLLKYIALGLGLKEDLFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG 240
           NLLKYIALGLGLKEDLFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG
Sbjct: 181 NLLKYIALGLGLKEDLFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG 240

Query: 241 KGSSVGLQILKDDKWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKHTDRLSLV 300
           KGSSVGLQILKD KWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKHTDRLS+V
Sbjct: 241 KGSSVGLQILKDGKWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKHTDRLSIV 300

Query: 301 TFYAPSYDIELGPMPEFVDKNNPCKYRRYNHGEYSKHYVTNKLQGKRTLEFAKIPIKNSC 360
           TFYAPSYDIELGPMPEFVDKNNPC+YRRYNHGEYSKHYVTNKLQGKRTLEFAKIP KNSC
Sbjct: 301 TFYAPSYDIELGPMPEFVDKNNPCRYRRYNHGEYSKHYVTNKLQGKRTLEFAKIPTKNSC 360

BLAST of Chy6G112130 vs. ExPASy TrEMBL
Match: A0A1S3AY19 (protein SRG1 OS=Cucumis melo OX=3656 GN=LOC103483830 PE=3 SV=1)

HSP 1 Score: 728.4 bits (1879), Expect = 1.5e-206
Identity = 350/361 (96.95%), Postives = 355/361 (98.34%), Query Frame = 0

Query: 1   MAPVPISPIKVGHIDDVQELRKFKPNIIPERFIRDIQERPTPATPLISSSDFPTIDLSKL 60
           MAPVPISPIKVGHIDDVQELRKFKPNIIPERFIRDIQERPTPATPLISSSD PTIDLSKL
Sbjct: 1   MAPVPISPIKVGHIDDVQELRKFKPNIIPERFIRDIQERPTPATPLISSSDIPTIDLSKL 60

Query: 61  LKGNRDEILQLATACEEWGFFQVINHGIALNLLENIEGRAMEFFRLPLEEKQKYAMVPGT 120
           LKGN+DEILQL TACEEWGFFQVINHGIALNLLENIEG+AMEFFRLPLEEKQKYAMVPGT
Sbjct: 61  LKGNKDEILQLTTACEEWGFFQVINHGIALNLLENIEGQAMEFFRLPLEEKQKYAMVPGT 120

Query: 121 VQGYGQAFVFSEHQKLDWCNMFALGVIPDFIRNPLFWPKKPANFSNTVETYSKEVRKLCK 180
           VQGYGQAFVFSEHQKLDWCNMFALGVIPDF+RNPLFWPKKPANFSNTVE YSKEVRKLCK
Sbjct: 121 VQGYGQAFVFSEHQKLDWCNMFALGVIPDFLRNPLFWPKKPANFSNTVEIYSKEVRKLCK 180

Query: 181 NLLKYIALGLGLKEDLFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG 240
           NLLKYIALGLGLKEDLFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG
Sbjct: 181 NLLKYIALGLGLKEDLFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG 240

Query: 241 KGSSVGLQILKDDKWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKHTDRLSLV 300
           KGSSVGLQILKD KWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKHTDRLS+V
Sbjct: 241 KGSSVGLQILKDGKWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKHTDRLSIV 300

Query: 301 TFYAPSYDIELGPMPEFVDKNNPCKYRRYNHGEYSKHYVTNKLQGKRTLEFAK-IPIKNS 360
           TFYAPSYDIELGPMPEFVDKNNPC+YRRYNHGEYSKHYVTNKLQGKRTLEFAK IP KNS
Sbjct: 301 TFYAPSYDIELGPMPEFVDKNNPCRYRRYNHGEYSKHYVTNKLQGKRTLEFAKIIPTKNS 360

BLAST of Chy6G112130 vs. ExPASy TrEMBL
Match: A0A5A7U796 (Protein SRG1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G007280 PE=3 SV=1)

HSP 1 Score: 726.5 bits (1874), Expect = 5.5e-206
Identity = 349/361 (96.68%), Postives = 354/361 (98.06%), Query Frame = 0

Query: 1   MAPVPISPIKVGHIDDVQELRKFKPNIIPERFIRDIQERPTPATPLISSSDFPTIDLSKL 60
           MAPVPISPIKVGHIDDVQELRKFKPNIIPERFIRDIQERPTPATPLISSSD PTIDLSKL
Sbjct: 1   MAPVPISPIKVGHIDDVQELRKFKPNIIPERFIRDIQERPTPATPLISSSDIPTIDLSKL 60

Query: 61  LKGNRDEILQLATACEEWGFFQVINHGIALNLLENIEGRAMEFFRLPLEEKQKYAMVPGT 120
           LKGN+DEILQL TACEEWGFFQVINHGIALNLLENIEG+AMEFFRLPLEEKQKYAMVPGT
Sbjct: 61  LKGNKDEILQLTTACEEWGFFQVINHGIALNLLENIEGQAMEFFRLPLEEKQKYAMVPGT 120

Query: 121 VQGYGQAFVFSEHQKLDWCNMFALGVIPDFIRNPLFWPKKPANF-SNTVETYSKEVRKLC 180
           VQGYGQAFVFSEHQKLDWCNMFALGVIPDF+RNPLFWPKKPANF  NTVE YSKEVRKLC
Sbjct: 121 VQGYGQAFVFSEHQKLDWCNMFALGVIPDFLRNPLFWPKKPANFRCNTVEIYSKEVRKLC 180

Query: 181 KNLLKYIALGLGLKEDLFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQ 240
           KNLLKYIALGLGLKEDLFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQ
Sbjct: 181 KNLLKYIALGLGLKEDLFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQ 240

Query: 241 GKGSSVGLQILKDDKWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKHTDRLSL 300
           GKGSSVGLQILKD KWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKHTDRLS+
Sbjct: 241 GKGSSVGLQILKDGKWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKHTDRLSI 300

Query: 301 VTFYAPSYDIELGPMPEFVDKNNPCKYRRYNHGEYSKHYVTNKLQGKRTLEFAKIPIKNS 360
           VTFYAPSYDIELGPMPEFVDKNNPC+YRRYNHGEYSKHYVTNKLQGKRTLEFAKIP KNS
Sbjct: 301 VTFYAPSYDIELGPMPEFVDKNNPCRYRRYNHGEYSKHYVTNKLQGKRTLEFAKIPTKNS 360

BLAST of Chy6G112130 vs. ExPASy TrEMBL
Match: A0A0A0L8Z7 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G153170 PE=3 SV=1)

HSP 1 Score: 706.4 bits (1822), Expect = 5.9e-200
Identity = 336/358 (93.85%), Postives = 344/358 (96.09%), Query Frame = 0

Query: 1   MAPVPISPIKVGHIDDVQELRKFKPNIIPERFIRDIQERPTPATPLISSSDFPTIDLSKL 60
           MAP PISPIK GHI DVQELRKF+PNIIPERFIRDIQERP PATPLISSSD PTIDLSKL
Sbjct: 1   MAPGPISPIKQGHIQDVQELRKFEPNIIPERFIRDIQERPAPATPLISSSDIPTIDLSKL 60

Query: 61  LKGNRDEILQLATACEEWGFFQVINHGIALNLLENIEGRAMEFFRLPLEEKQKYAMVPGT 120
           LKGNRDE+LQLATACEEWGFFQVINHGIALNLLENIEG+AMEFFRLPLEEKQKYAM PGT
Sbjct: 61  LKGNRDELLQLATACEEWGFFQVINHGIALNLLENIEGQAMEFFRLPLEEKQKYAMAPGT 120

Query: 121 VQGYGQAFVFSEHQKLDWCNMFALGVIPDFIRNPLFWPKKPANFSNTVETYSKEVRKLCK 180
           VQGYGQAFVFSEHQKLDWCNMFALG+ P+++RNPL WP KPANFSNTVE YSKEVRKLCK
Sbjct: 121 VQGYGQAFVFSEHQKLDWCNMFALGITPEYLRNPLLWPNKPANFSNTVEIYSKEVRKLCK 180

Query: 181 NLLKYIALGLGLKEDLFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG 240
           NLLKYIAL LGLKEDLFEEAFG AVQA+RMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG
Sbjct: 181 NLLKYIALSLGLKEDLFEEAFGAAVQAVRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG 240

Query: 241 KGSSVGLQILKDDKWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKHTDRLSLV 300
           KG SVGLQILKDDKWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKHTDRLSLV
Sbjct: 241 KGCSVGLQILKDDKWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKHTDRLSLV 300

Query: 301 TFYAPSYDIELGPMPEFVDKNNPCKYRRYNHGEYSKHYVTNKLQGKRTLEFAKIPIKN 359
           TFYAPSYDIELGPMPEFVDKNNPCKYRRYNHGEYSKHYV NKLQGKRTLEFAKIPIKN
Sbjct: 301 TFYAPSYDIELGPMPEFVDKNNPCKYRRYNHGEYSKHYVANKLQGKRTLEFAKIPIKN 358

BLAST of Chy6G112130 vs. ExPASy TrEMBL
Match: A0A6J1IXQ5 (protein SRG1-like OS=Cucurbita maxima OX=3661 GN=LOC111479417 PE=3 SV=1)

HSP 1 Score: 694.9 bits (1792), Expect = 1.8e-196
Identity = 332/360 (92.22%), Postives = 341/360 (94.72%), Query Frame = 0

Query: 1   MAPVPISPIKVGHIDDVQELRKFKPNIIPERFIRDIQERPTPATPLISSSDFPTIDLSKL 60
           MAP PISPI VGHIDDVQELRKFKPN IP+RFIRDIQERPT AT LISSSD PTIDLSKL
Sbjct: 1   MAPAPISPINVGHIDDVQELRKFKPNTIPQRFIRDIQERPTQATALISSSDIPTIDLSKL 60

Query: 61  LKGNRDEILQLATACEEWGFFQVINHGIALNLLENIEGRAMEFFRLPLEEKQKYAMVPGT 120
           LKGNRDEIL L +ACEEWGFFQVINHGIALNLLENIEG+AMEFFRLPLEEKQKY M PGT
Sbjct: 61  LKGNRDEILHLYSACEEWGFFQVINHGIALNLLENIEGKAMEFFRLPLEEKQKYQMAPGT 120

Query: 121 VQGYGQAFVFSEHQKLDWCNMFALGVIPDFIRNPLFWPKKPANFSNTVETYSKEVRKLCK 180
           VQGYGQAFVFSEHQKLDWCNMFALGVIPD IRN L WPKKP NFSNTVE YSKEVRKLC+
Sbjct: 121 VQGYGQAFVFSEHQKLDWCNMFALGVIPDSIRNNLLWPKKPTNFSNTVEIYSKEVRKLCQ 180

Query: 181 NLLKYIALGLGLKEDLFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG 240
           NLLKYIALGLGLK+DLFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGS LTVLQQG
Sbjct: 181 NLLKYIALGLGLKKDLFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSVLTVLQQG 240

Query: 241 KGSSVGLQILKDDKWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKHTDRLSLV 300
           KGSSVGLQILKDDKWVPVQPIP+A VINIGDTMEVVTNGR+KSVEHRAVTHKH+DRLS+V
Sbjct: 241 KGSSVGLQILKDDKWVPVQPIPDAFVINIGDTMEVVTNGRFKSVEHRAVTHKHSDRLSIV 300

Query: 301 TFYAPSYDIELGPMPEFVDKNNPCKYRRYNHGEYSKHYVTNKLQGKRTLEFAKIPIKNSC 360
           TFYAPSYDIELGPMPEFVDK NPCKYRRYNHGEYSKHYVTNKLQGKRTLEFAKIP KNSC
Sbjct: 301 TFYAPSYDIELGPMPEFVDKKNPCKYRRYNHGEYSKHYVTNKLQGKRTLEFAKIPTKNSC 360

BLAST of Chy6G112130 vs. NCBI nr
Match: TYK16155.1 (protein SRG1 [Cucumis melo var. makuwa])

HSP 1 Score: 728 bits (1878), Expect = 8.41e-265
Identity = 350/360 (97.22%), Postives = 355/360 (98.61%), Query Frame = 0

Query: 1   MAPVPISPIKVGHIDDVQELRKFKPNIIPERFIRDIQERPTPATPLISSSDFPTIDLSKL 60
           MAPVPISPIKVGHIDDVQELRKFKPNIIPERFIRDIQERPTPATPLISSSD PTIDLSKL
Sbjct: 1   MAPVPISPIKVGHIDDVQELRKFKPNIIPERFIRDIQERPTPATPLISSSDIPTIDLSKL 60

Query: 61  LKGNRDEILQLATACEEWGFFQVINHGIALNLLENIEGRAMEFFRLPLEEKQKYAMVPGT 120
           LKGN+DEILQL TACEEWGFFQVINHGIALNLLENIEG+AMEFFRLPLEEKQKYAMVPGT
Sbjct: 61  LKGNKDEILQLTTACEEWGFFQVINHGIALNLLENIEGQAMEFFRLPLEEKQKYAMVPGT 120

Query: 121 VQGYGQAFVFSEHQKLDWCNMFALGVIPDFIRNPLFWPKKPANFSNTVETYSKEVRKLCK 180
           VQGYGQAFVFSEHQKLDWCNMFALGVIPDF+RNPLFWPKKPANFSNTVE YSKEVRKLCK
Sbjct: 121 VQGYGQAFVFSEHQKLDWCNMFALGVIPDFLRNPLFWPKKPANFSNTVEIYSKEVRKLCK 180

Query: 181 NLLKYIALGLGLKEDLFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG 240
           NLLKYIALGLGLKEDLFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG
Sbjct: 181 NLLKYIALGLGLKEDLFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG 240

Query: 241 KGSSVGLQILKDDKWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKHTDRLSLV 300
           KGSSVGLQILKD KWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKHTDRLS+V
Sbjct: 241 KGSSVGLQILKDGKWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKHTDRLSIV 300

Query: 301 TFYAPSYDIELGPMPEFVDKNNPCKYRRYNHGEYSKHYVTNKLQGKRTLEFAKIPIKNSC 360
           TFYAPSYDIELGPMPEFVDKNNPC+YRRYNHGEYSKHYVTNKLQGKRTLEFAKIP KNSC
Sbjct: 301 TFYAPSYDIELGPMPEFVDKNNPCRYRRYNHGEYSKHYVTNKLQGKRTLEFAKIPTKNSC 360

BLAST of Chy6G112130 vs. NCBI nr
Match: XP_008438869.1 (PREDICTED: protein SRG1 [Cucumis melo])

HSP 1 Score: 723 bits (1866), Expect = 5.90e-263
Identity = 350/361 (96.95%), Postives = 355/361 (98.34%), Query Frame = 0

Query: 1   MAPVPISPIKVGHIDDVQELRKFKPNIIPERFIRDIQERPTPATPLISSSDFPTIDLSKL 60
           MAPVPISPIKVGHIDDVQELRKFKPNIIPERFIRDIQERPTPATPLISSSD PTIDLSKL
Sbjct: 1   MAPVPISPIKVGHIDDVQELRKFKPNIIPERFIRDIQERPTPATPLISSSDIPTIDLSKL 60

Query: 61  LKGNRDEILQLATACEEWGFFQVINHGIALNLLENIEGRAMEFFRLPLEEKQKYAMVPGT 120
           LKGN+DEILQL TACEEWGFFQVINHGIALNLLENIEG+AMEFFRLPLEEKQKYAMVPGT
Sbjct: 61  LKGNKDEILQLTTACEEWGFFQVINHGIALNLLENIEGQAMEFFRLPLEEKQKYAMVPGT 120

Query: 121 VQGYGQAFVFSEHQKLDWCNMFALGVIPDFIRNPLFWPKKPANFSNTVETYSKEVRKLCK 180
           VQGYGQAFVFSEHQKLDWCNMFALGVIPDF+RNPLFWPKKPANFSNTVE YSKEVRKLCK
Sbjct: 121 VQGYGQAFVFSEHQKLDWCNMFALGVIPDFLRNPLFWPKKPANFSNTVEIYSKEVRKLCK 180

Query: 181 NLLKYIALGLGLKEDLFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG 240
           NLLKYIALGLGLKEDLFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG
Sbjct: 181 NLLKYIALGLGLKEDLFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG 240

Query: 241 KGSSVGLQILKDDKWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKHTDRLSLV 300
           KGSSVGLQILKD KWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKHTDRLS+V
Sbjct: 241 KGSSVGLQILKDGKWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKHTDRLSIV 300

Query: 301 TFYAPSYDIELGPMPEFVDKNNPCKYRRYNHGEYSKHYVTNKLQGKRTLEFAKI-PIKNS 360
           TFYAPSYDIELGPMPEFVDKNNPC+YRRYNHGEYSKHYVTNKLQGKRTLEFAKI P KNS
Sbjct: 301 TFYAPSYDIELGPMPEFVDKNNPCRYRRYNHGEYSKHYVTNKLQGKRTLEFAKIIPTKNS 360

BLAST of Chy6G112130 vs. NCBI nr
Match: KAA0049475.1 (protein SRG1 [Cucumis melo var. makuwa])

HSP 1 Score: 721 bits (1861), Expect = 3.41e-262
Identity = 349/361 (96.68%), Postives = 354/361 (98.06%), Query Frame = 0

Query: 1   MAPVPISPIKVGHIDDVQELRKFKPNIIPERFIRDIQERPTPATPLISSSDFPTIDLSKL 60
           MAPVPISPIKVGHIDDVQELRKFKPNIIPERFIRDIQERPTPATPLISSSD PTIDLSKL
Sbjct: 1   MAPVPISPIKVGHIDDVQELRKFKPNIIPERFIRDIQERPTPATPLISSSDIPTIDLSKL 60

Query: 61  LKGNRDEILQLATACEEWGFFQVINHGIALNLLENIEGRAMEFFRLPLEEKQKYAMVPGT 120
           LKGN+DEILQL TACEEWGFFQVINHGIALNLLENIEG+AMEFFRLPLEEKQKYAMVPGT
Sbjct: 61  LKGNKDEILQLTTACEEWGFFQVINHGIALNLLENIEGQAMEFFRLPLEEKQKYAMVPGT 120

Query: 121 VQGYGQAFVFSEHQKLDWCNMFALGVIPDFIRNPLFWPKKPANFS-NTVETYSKEVRKLC 180
           VQGYGQAFVFSEHQKLDWCNMFALGVIPDF+RNPLFWPKKPANF  NTVE YSKEVRKLC
Sbjct: 121 VQGYGQAFVFSEHQKLDWCNMFALGVIPDFLRNPLFWPKKPANFRCNTVEIYSKEVRKLC 180

Query: 181 KNLLKYIALGLGLKEDLFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQ 240
           KNLLKYIALGLGLKEDLFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQ
Sbjct: 181 KNLLKYIALGLGLKEDLFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQ 240

Query: 241 GKGSSVGLQILKDDKWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKHTDRLSL 300
           GKGSSVGLQILKD KWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKHTDRLS+
Sbjct: 241 GKGSSVGLQILKDGKWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKHTDRLSI 300

Query: 301 VTFYAPSYDIELGPMPEFVDKNNPCKYRRYNHGEYSKHYVTNKLQGKRTLEFAKIPIKNS 360
           VTFYAPSYDIELGPMPEFVDKNNPC+YRRYNHGEYSKHYVTNKLQGKRTLEFAKIP KNS
Sbjct: 301 VTFYAPSYDIELGPMPEFVDKNNPCRYRRYNHGEYSKHYVTNKLQGKRTLEFAKIPTKNS 360

BLAST of Chy6G112130 vs. NCBI nr
Match: XP_038905272.1 (protein SRG1 [Benincasa hispida])

HSP 1 Score: 708 bits (1827), Expect = 5.01e-257
Identity = 341/360 (94.72%), Postives = 347/360 (96.39%), Query Frame = 0

Query: 1   MAPVPISPIKVGHIDDVQELRKFKPNIIPERFIRDIQERPTPATPLISSSDFPTIDLSKL 60
           MAPVPISPI VGHIDDVQELRKFKPN IPERFIRDIQERPTPATPLISSSD PTIDLSKL
Sbjct: 1   MAPVPISPINVGHIDDVQELRKFKPNTIPERFIRDIQERPTPATPLISSSDIPTIDLSKL 60

Query: 61  LKGNRDEILQLATACEEWGFFQVINHGIALNLLENIEGRAMEFFRLPLEEKQKYAMVPGT 120
           LKGNRDEILQL TACEEWGFFQVINHGI+LNLLENIEG+A+EFFRLPLEEKQKYAM PGT
Sbjct: 61  LKGNRDEILQLTTACEEWGFFQVINHGISLNLLENIEGQALEFFRLPLEEKQKYAMAPGT 120

Query: 121 VQGYGQAFVFSEHQKLDWCNMFALGVIPDFIRNPLFWPKKPANFSNTVETYSKEVRKLCK 180
           VQGYGQAFVFSEHQKLDWCNM ALGVIPD IRNPL WPKKP NFSNTVE YSKEVRKLCK
Sbjct: 121 VQGYGQAFVFSEHQKLDWCNMLALGVIPDSIRNPLLWPKKPTNFSNTVEIYSKEVRKLCK 180

Query: 181 NLLKYIALGLGLKEDLFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG 240
           NLLKYIALGLGLKE+LFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG
Sbjct: 181 NLLKYIALGLGLKENLFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG 240

Query: 241 KGSSVGLQILKDDKWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKHTDRLSLV 300
           KGSSVGLQIL+DDKWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHK TDRLS+V
Sbjct: 241 KGSSVGLQILRDDKWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKQTDRLSIV 300

Query: 301 TFYAPSYDIELGPMPEFVDKNNPCKYRRYNHGEYSKHYVTNKLQGKRTLEFAKIPIKNSC 360
           TFYAPSYDIELGPMPEFVDKNNPC YRRYNHGEYSKHYVTNKLQGKRTLEFAKIP KNSC
Sbjct: 301 TFYAPSYDIELGPMPEFVDKNNPCMYRRYNHGEYSKHYVTNKLQGKRTLEFAKIPTKNSC 360

BLAST of Chy6G112130 vs. NCBI nr
Match: XP_004134481.1 (protein SRG1 [Cucumis sativus] >KGN57092.1 hypothetical protein Csa_011608 [Cucumis sativus])

HSP 1 Score: 701 bits (1810), Expect = 1.81e-254
Identity = 336/358 (93.85%), Postives = 344/358 (96.09%), Query Frame = 0

Query: 1   MAPVPISPIKVGHIDDVQELRKFKPNIIPERFIRDIQERPTPATPLISSSDFPTIDLSKL 60
           MAP PISPIK GHI DVQELRKF+PNIIPERFIRDIQERP PATPLISSSD PTIDLSKL
Sbjct: 1   MAPGPISPIKQGHIQDVQELRKFEPNIIPERFIRDIQERPAPATPLISSSDIPTIDLSKL 60

Query: 61  LKGNRDEILQLATACEEWGFFQVINHGIALNLLENIEGRAMEFFRLPLEEKQKYAMVPGT 120
           LKGNRDE+LQLATACEEWGFFQVINHGIALNLLENIEG+AMEFFRLPLEEKQKYAM PGT
Sbjct: 61  LKGNRDELLQLATACEEWGFFQVINHGIALNLLENIEGQAMEFFRLPLEEKQKYAMAPGT 120

Query: 121 VQGYGQAFVFSEHQKLDWCNMFALGVIPDFIRNPLFWPKKPANFSNTVETYSKEVRKLCK 180
           VQGYGQAFVFSEHQKLDWCNMFALG+ P+++RNPL WP KPANFSNTVE YSKEVRKLCK
Sbjct: 121 VQGYGQAFVFSEHQKLDWCNMFALGITPEYLRNPLLWPNKPANFSNTVEIYSKEVRKLCK 180

Query: 181 NLLKYIALGLGLKEDLFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG 240
           NLLKYIAL LGLKEDLFEEAFG AVQA+RMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG
Sbjct: 181 NLLKYIALSLGLKEDLFEEAFGAAVQAVRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG 240

Query: 241 KGSSVGLQILKDDKWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKHTDRLSLV 300
           KG SVGLQILKDDKWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKHTDRLSLV
Sbjct: 241 KGCSVGLQILKDDKWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKHTDRLSLV 300

Query: 301 TFYAPSYDIELGPMPEFVDKNNPCKYRRYNHGEYSKHYVTNKLQGKRTLEFAKIPIKN 358
           TFYAPSYDIELGPMPEFVDKNNPCKYRRYNHGEYSKHYV NKLQGKRTLEFAKIPIKN
Sbjct: 301 TFYAPSYDIELGPMPEFVDKNNPCKYRRYNHGEYSKHYVANKLQGKRTLEFAKIPIKN 358

BLAST of Chy6G112130 vs. TAIR 10
Match: AT3G21420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 516.2 bits (1328), Expect = 2.2e-146
Identity = 250/362 (69.06%), Postives = 296/362 (81.77%), Query Frame = 0

Query: 1   MAPVPISPIKVGHIDDVQELRKFKPNIIPERFIRDIQERPTPATPLIS---SSDFPTIDL 60
           MAP+PIS I+VG IDDVQEL K KPN +PERFIR+  ER    + L +       P IDL
Sbjct: 1   MAPLPISSIRVGKIDDVQELIKSKPNKVPERFIREEYERGVVVSSLKTHHLHHQIPVIDL 60

Query: 61  SKLLKGNRD----EILQLATACEEWGFFQVINHGIALNLLENIEGRAMEFFRLPLEEKQK 120
           SKL K + D    EIL+L+ ACE+WGFFQVINHGI + ++E+IE  A EFF +PLEEK+K
Sbjct: 61  SKLSKPDNDDFFFEILKLSQACEDWGFFQVINHGIEVEVVEDIEEVASEFFDMPLEEKKK 120

Query: 121 YAMVPGTVQGYGQAFVFSEHQKLDWCNMFALGVIPDFIRNPLFWPKKPANFSNTVETYSK 180
           Y M PGTVQGYGQAF+FSE QKLDWCNMFALGV P  IRNP  WP KPA FS ++E YSK
Sbjct: 121 YPMEPGTVQGYGQAFIFSEDQKLDWCNMFALGVHPPQIRNPKLWPSKPARFSESLEGYSK 180

Query: 181 EVRKLCKNLLKYIALGLGLKEDLFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSA 240
           E+R+LCK LLKYIA+ LGLKE+ FEE FG AVQA+RMNYYPPCS PDLVLGLSPHSDGSA
Sbjct: 181 EIRELCKRLLKYIAISLGLKEERFEEMFGEAVQAVRMNYYPPCSSPDLVLGLSPHSDGSA 240

Query: 241 LTVLQQGKGSSVGLQILKDDKWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKH 300
           LTVLQQ K S VGLQILKD+ WVPV+P+PNALVINIGDT+EV++NG+YKSVEHRAVT++ 
Sbjct: 241 LTVLQQSKNSCVGLQILKDNTWVPVKPLPNALVINIGDTIEVLSNGKYKSVEHRAVTNRE 300

Query: 301 TDRLSLVTFYAPSYDIELGPMPEFV-DKNNPCKYRRYNHGEYSKHYVTNKLQGKRTLEFA 355
            +RL++VTFYAP+Y++E+ PM E V D+ NPCKYR YNHG+YS HYV+NKLQGK++L+FA
Sbjct: 301 KERLTIVTFYAPNYEVEIEPMSELVDDETNPCKYRSYNHGDYSYHYVSNKLQGKKSLDFA 360

BLAST of Chy6G112130 vs. TAIR 10
Match: AT1G17020.1 (senescence-related gene 1 )

HSP 1 Score: 286.6 bits (732), Expect = 2.8e-77
Identity = 142/346 (41.04%), Postives = 210/346 (60.69%), Query Frame = 0

Query: 14  IDDVQELRKFKP-NIIPERFIRDIQERPTPATPLISSSDFPTIDLSKLLKGNR--DEILQ 73
           +  VQE+ K K    +P R++R  Q++           + P ID+ +L        E+ +
Sbjct: 14  VPSVQEMVKEKTITTVPPRYVRSDQDKTEVDDDFDVKIEIPIIDMKRLCSSTTMDSEVEK 73

Query: 74  LATACEEWGFFQVINHGIALNLLENIEGRAMEFFRLPLEEKQKYAMVPGTVQGYGQAFVF 133
           L  AC+EWGFFQ++NHGI  + L+ ++    +FF LP+EEK+K+   P  ++G+GQAFV 
Sbjct: 74  LDFACKEWGFFQLVNHGIDSSFLDKVKSEIQDFFNLPMEEKKKFWQRPDEIEGFGQAFVV 133

Query: 134 SEHQKLDWCNMFALGVIPDFIRNPLFWPKKPANFSNTVETYSKEVRKLCKNLLKYIALGL 193
           SE QKLDW ++F   V P  +R P  +PK P  F +T+E YS EV+ + K L+  +A  L
Sbjct: 134 SEDQKLDWADLFFHTVQPVELRKPHLFPKLPLPFRDTLEMYSSEVQSVAKILIAKMARAL 193

Query: 194 GLKEDLFEEAFG--VAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQGKGSSVGLQ 253
            +K +  E+ F    +VQ++RMNYYPPC +PD V+GL+PHSD   LTVL Q      GLQ
Sbjct: 194 EIKPEELEKLFDDVDSVQSMRMNYYPPCPQPDQVIGLTPHSDSVGLTVLMQ-VNDVEGLQ 253

Query: 254 ILKDDKWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKHTDRLSLVTFYAPSYD 313
           I KD KWVPV+P+PNA ++NIGD +E++TNG Y+S+EHR V +   +RLS+ TF+     
Sbjct: 254 IKKDGKWVPVKPLPNAFIVNIGDVLEIITNGTYRSIEHRGVVNSEKERLSIATFHNVGMY 313

Query: 314 IELGPMPEFVDKNNPCKYRRYNHGEYSKHYVTNKLQGKRTLEFAKI 355
            E+GP    V++    +++R    EY+    +  L GK  L+  +I
Sbjct: 314 KEVGPAKSLVERQKVARFKRLTMKEYNDGLFSRTLDGKAYLDALRI 358

BLAST of Chy6G112130 vs. TAIR 10
Match: AT4G25310.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 279.6 bits (714), Expect = 3.5e-75
Identity = 145/349 (41.55%), Postives = 211/349 (60.46%), Query Frame = 0

Query: 14  IDDVQELRKFK--PNIIPERFIRDIQERPTPATPLISSSDFPTIDLSKLLKGNR--DEIL 73
           +  VQE+ K K    ++P R++R  QE+   A      +  P ID+S L        EI 
Sbjct: 13  VPSVQEMVKEKVITTVLPPRYVRSDQEKGEAAID-SGENQIPIIDMSLLSSSTSMDSEID 72

Query: 74  QLATACEEWGFFQVINHGIALNLLENIEGRAMEFFRLPLEEKQKYAMVPGTVQGYGQAFV 133
           +L  AC+EWGFFQ++NHG+    L+  +    +FF LP+EEK+K    PG ++G+GQAFV
Sbjct: 73  KLDFACKEWGFFQLVNHGMD---LDKFKSDIQDFFNLPMEEKKKLWQQPGDIEGFGQAFV 132

Query: 134 FSEHQKLDWCNMFALGVIPDFIRNPLFWPKKPANFSNTVETYSKEVRKLCKNLLKYIALG 193
           FSE QKLDW ++F L + P  +R P  +PK P  F +T++TYS E++ + K L   +A  
Sbjct: 133 FSEEQKLDWADVFFLTMQPVPLRKPHLFPKLPLPFRDTLDTYSAELKSIAKVLFAKLASA 192

Query: 194 LGLK----EDLFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQGKGSSV 253
           L +K    E LF++  G   Q IRMNYYPPC  PD  +GL+PHSD + LT+L Q      
Sbjct: 193 LKIKPEEMEKLFDDELG---QRIRMNYYPPCPEPDKAIGLTPHSDATGLTILLQ-VNEVE 252

Query: 254 GLQILKDDKWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKHTDRLSLVTFYAP 313
           GLQI KD KWV V+P+PNALV+N+GD +E++TNG Y+S+EHR V +   +RLS+ +F+  
Sbjct: 253 GLQIKKDGKWVSVKPLPNALVVNVGDILEIITNGTYRSIEHRGVVNSEKERLSVASFHNT 312

Query: 314 SYDIELGPMPEFVDKNNPCKYRRYNHGEYSKHYVTNKLQGKRTLEFAKI 355
            +  E+GPM   V+++    ++     EY     + +L GK  L+  +I
Sbjct: 313 GFGKEIGPMRSLVERHKGALFKTLTTEEYFHGLFSRELDGKAYLDVMRI 353

BLAST of Chy6G112130 vs. TAIR 10
Match: AT1G78550.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 275.0 bits (702), Expect = 8.5e-74
Identity = 136/329 (41.34%), Postives = 200/329 (60.79%), Query Frame = 0

Query: 28  IPERFIRDIQERPTPATPLISSSDFPTIDLSKL--LKGNRDEILQLATACEEWGFFQVIN 87
           IP R++R  QE+         SS+ P ID+++L  +     E+ +L  AC++WGFFQ++N
Sbjct: 29  IPPRYVRVDQEKTEILNDSSLSSEIPVIDMTRLCSVSAMDSELKKLDFACQDWGFFQLVN 88

Query: 88  HGIALNLLENIEGRAMEFFRLPLEEKQKYAMVPGTVQGYGQAFVFSEHQKLDWCNMFALG 147
           HGI  + LE +E    EFF LP++EKQK     G  +G+GQ  + SE+QKLDW +MF L 
Sbjct: 89  HGIDSSFLEKLETEVQEFFNLPMKEKQKLWQRSGEFEGFGQVNIVSENQKLDWGDMFILT 148

Query: 148 VIPDFIRNPLFWPKKPANFSNTVETYSKEVRKLCKNLLKYIALGLGLKEDLFEEAFGVAV 207
             P   R    + K P  F  T+ETYS EV+ + K L   +A  L +K +  E+ F    
Sbjct: 149 TEPIRSRKSHLFSKLPPPFRETLETYSSEVKSIAKILFAKMASVLEIKHEEMEDLFDDVW 208

Query: 208 QAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQGKGSSVGLQILKDDKWVPVQPIPNAL 267
           Q+I++NYYPPC +PD V+GL+ HSD + LT+L Q      GLQI KD KWV V+P+ +AL
Sbjct: 209 QSIKINYYPPCPQPDQVMGLTQHSDAAGLTILLQ-VNQVEGLQIKKDGKWVVVKPLRDAL 268

Query: 268 VINIGDTMEVVTNGRYKSVEHRAVTHKHTDRLSLVTFYAPSYDIELGPMPEFVDKNNPCK 327
           V+N+G+ +E++TNGRY+S+EHRAV +   +RLS+  F++P  +  + P    VD+   C 
Sbjct: 269 VVNVGEILEIITNGRYRSIEHRAVVNSEKERLSVAMFHSPGKETIIRPAKSLVDRQKQCL 328

Query: 328 YRRYNHGEYSKHYVTNKLQGKRTLEFAKI 355
           ++  +  EY   + T KL GK  L+  +I
Sbjct: 329 FKSMSTQEYFDAFFTQKLNGKSHLDLMRI 356

BLAST of Chy6G112130 vs. TAIR 10
Match: AT4G25300.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 271.9 bits (694), Expect = 7.2e-73
Identity = 139/348 (39.94%), Postives = 206/348 (59.20%), Query Frame = 0

Query: 14  IDDVQELRKFKP-NIIPERFIRDIQERPTPATPLISSSDFPTIDLSKLLKGNR--DEILQ 73
           +  VQE+ K K    +P R++R  Q+    A      +  P ID+S L        EI +
Sbjct: 13  VPSVQEMVKEKMITTVPPRYVRSDQDVAEIAVDSGLRNQIPIIDMSLLCSSTSMDSEIDK 72

Query: 74  LATACEEWGFFQVINHGIALNLLENIEGRAMEFFRLPLEEKQKYAMVPGTVQGYGQAFVF 133
           L +AC+EWGFFQ++NHG+  + L  ++    +FF LP+EEK+     P  ++G+GQ FV 
Sbjct: 73  LDSACKEWGFFQLVNHGMESSFLNKVKSEVQDFFNLPMEEKKNLWQQPDEIEGFGQVFVV 132

Query: 134 SEHQKLDWCNMFALGVIPDFIRNPLFWPKKPANFSNTVETYSKEVRKLCKNLLKYIALGL 193
           SE QKLDW +MF L + P  +R P  +PK P  F +T++ YS EV+ + K LL  IA+ L
Sbjct: 133 SEEQKLDWADMFFLTMQPVRLRKPHLFPKLPLPFRDTLDMYSAEVKSIAKILLGKIAVAL 192

Query: 194 GLK----EDLFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQGKGSSVG 253
            +K    + LF++  G   Q IR+NYYP C  PD V+GL+PHSD + LT+L Q      G
Sbjct: 193 KIKPEEMDKLFDDELG---QRIRLNYYPRCPEPDKVIGLTPHSDSTGLTILLQA-NEVEG 252

Query: 254 LQILKDDKWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKHTDRLSLVTFYAPS 313
           LQI K+ KWV V+P+PNALV+N+GD +E++TNG Y+S+EHR V +   +RLS+  F+   
Sbjct: 253 LQIKKNAKWVSVKPLPNALVVNVGDILEIITNGTYRSIEHRGVVNSEKERLSVAAFHNIG 312

Query: 314 YDIELGPMPEFVDKNNPCKYRRYNHGEYSKHYVTNKLQGKRTLEFAKI 355
              E+GPM   V+++    ++     EY     + +L GK  L+  ++
Sbjct: 313 LGKEIGPMRSLVERHKAAFFKSVTTEEYFNGLFSRELDGKAYLDVMRL 356

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q392244.0e-7641.04Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1[more]
Q94LP44.5e-7238.182-oxoglutarate-dependent dioxygenase 11 OS=Oryza sativa subsp. japonica OX=39947... [more]
D4N5011.9e-7038.90Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Papaver somniferum OX=34... [more]
O804499.5e-7039.38Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1[more]
D4N5024.7e-6938.33Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3CXG45.9e-20897.22Protein SRG1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G00820 P... [more]
A0A1S3AY191.5e-20696.95protein SRG1 OS=Cucumis melo OX=3656 GN=LOC103483830 PE=3 SV=1[more]
A0A5A7U7965.5e-20696.68Protein SRG1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G007280 ... [more]
A0A0A0L8Z75.9e-20093.85Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G... [more]
A0A6J1IXQ51.8e-19692.22protein SRG1-like OS=Cucurbita maxima OX=3661 GN=LOC111479417 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
TYK16155.18.41e-26597.22protein SRG1 [Cucumis melo var. makuwa][more]
XP_008438869.15.90e-26396.95PREDICTED: protein SRG1 [Cucumis melo][more]
KAA0049475.13.41e-26296.68protein SRG1 [Cucumis melo var. makuwa][more]
XP_038905272.15.01e-25794.72protein SRG1 [Benincasa hispida][more]
XP_004134481.11.81e-25493.85protein SRG1 [Cucumis sativus] >KGN57092.1 hypothetical protein Csa_011608 [Cucu... [more]
Match NameE-valueIdentityDescription
AT3G21420.12.2e-14669.062-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G17020.12.8e-7741.04senescence-related gene 1 [more]
AT4G25310.13.5e-7541.552-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G78550.18.5e-7441.342-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G25300.17.2e-7339.942-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 12..346
e-value: 3.7E-105
score: 353.9
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 53..159
e-value: 4.9E-26
score: 91.8
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 206..305
e-value: 6.5E-24
score: 84.3
NoneNo IPR availablePANTHERPTHR47991:SF9PROTEIN SRG1coord: 10..355
NoneNo IPR availablePANTHERPTHR47991OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASEcoord: 10..355
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 16..346
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 203..305
score: 13.2418

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy6G112130.1Chy6G112130.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1901601 strigolactone biosynthetic process
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding