Chy6G111710 (gene) Cucumber (hystrix) v1

Overview


Name	Chy6G111710
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Procollagen-proline 4-dioxygenase
Location	chrH06: 4356238 .. 4359058 (+)
RNA-Seq Expression	Chy6G111710
Synteny	Chy6G111710

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCGAATTTCTCTGTTTCAATCTACTTTTTCTCTTCACATTATCCATCTCCTTCCTTCTCCAGCGAGCTTCAGCCTCCTATGCAGGTTCCGCTAGCTCAATCGTCAACCCTGCAAAAGTCAAACAGATTTCATGGTCTCCTCGGTACTTCAATTTCTCCCTCTCCCTCTTCTTCTTCTACTACTACTACTACTACTTCCTAAGTTTTGATGATCGATTAGATTTGTTCACGGAACCCTGGTCCCGTTTCAATTTGAATGATTCTTCAGGGCTTTTGTGTATGAAGGTTTTCTCACGGATTTAGAATGCGATCATCTCATTTCCCTCGTGAGTAGAATTAGAATCGATTCGTTTTTTTCTTTTCGATATGCGATTTTTGTTTTGGTTGATTTTGGTTTTGTTTGAATTAGGCTAAAGCGGAGCTGAAGAGATCTTCTGTTGCGGATAATTTGTCCGGAAAGAGCAAAGTCAGCGAGGTTCGAACTAGCTCTGGGGCGTTTATTCATAAAGCCAAGGTTTGTTTAGACTTTAGAGTTTGCTACTTTTAGTTCTTAAGTTGCCAATAAGAGAACCATTGTGGCTTTATATATCATCTTTTAGTTTGAATCACTATCAAACCTAAATCTTAAACTCTCGAGCTTGAAATGTATTGGTTGGATATACCATTGAGATTTCCTCATGGGTTTAAAAATCAGTTTGGTTCATGTTCATGAGATCCATCTTTTGAATATTGGTCTGCTTTATGAAATATATTTTCAAATGAAATGAGTGATGAGCAAGTATGACATTGCAGTAATTTGATCTTCTGATCTTCCATTCTGTGATACTGGTATAAATCACCATCTGTGAAAAGCAAAAGAAAACAAAAAGGTTTGAACAGTTGGGTTGAAGCAACTTTCATCAGTTAATCTATTCATCAATCCGTATTGATTTGAAAGGGACAGCTGTTATTATTGCTTCATTCCGTATTAATTTGGATTATCGGTATCTTGAATGACAAATTTTTTAAGGATGCGTTTAGCAGCCTGAGCTGATTGAAATTTCAAATTTTCCTGTCCTAGTTGACTTTGTAGACGGAAAACATTAATGCTATCCTTGATCTTTCCGAGTATAGAGATAGGGAGCCAATTTTACTTATAAACACCTATCCTGATGAGGCACTCTCTTACTCCTTCTGTGTAAACTTATTATTGTACATCTGATGTAGGACCCTATTGTTTCTGGTATAGAAGACAAAATTGCAGCATGGACATTTCTGCCAAAAGGTATAATACCTCTAGTGGAAGCACGCAAATTTCTTGTCAAATCCCATTGTCTTTAGCAACTTATCCTAGATTGCGAACTCCTTTCCTTTATCTTAAACTAAAGATTGAATTGACTGAAAATTGACTGTTTCTTGTGACAACTATGGATGTTAACAGGATCTAGGCTTATGCAGTTGAACTCTGAATTTTGGGCTCTGAAGTTCTATCTATTCTCATGACTGAAACCATAAAGAGAAATCTTGCGCTAATTATTTTGAACTGTAATATCGAAAAAATAAATAGATAACGAAACGTTGTGTATCAGATAAGTTCTAGTCCTCATTTTTCCCGTGTGATTGTATAATTCTATCCGCAGGATTTTATTCCAAATTTCATATAAATTAGTGAACTTAGGAAGACATGATCCCCCGTCATAACTGTGAAATGCATAATCATCAATAGAGTCTGAGGAATAATTTTGCTGCTGGGAAGAAACAGAGTTTGGACTAAGCCTTCTCTTTTTTCTTTTTAGTGAGAAAAGAAAACTGTGGATATGAAACTCTATTTTTATAAGATGTGAAGCATTTGACTCGTTCATTAATAGATTGTTTGGGTATTGATATTACTGGTAAAGTGAAACTTGAATGTGCTTTATTTCAGAAAATGGCGAAGACATTCAAGTGTTGAGATATGAATATGGACAGAAGTACGATGCACACTTTGATTACTTTGCTGACAAGGTTAACATTGCCCGAGGTGGACATCGAATGGCAACCGTTCTCATGTATCTTTCCGACGTAGAAAAAGGCGGTGAAACTGTGTTTCCTTCTGCAGAGGTCCGTCCTTGTATTCCCCTTCAATCCAATTAATTGCAACTTCTTTTTTCTTTTTTAGCCTGAACCGTGACCATAATCAAGTGCCTTGGTTTTCTTAGTTCAATATTCTTTGCAGGAAGATATATCTGTCTATACTTGAATAACTAATTTGAATTGGAAAATTTTCCTGTCTTGGCATTTATATGTGCTGTCATTACTCATTTATAGATGACTTTTTATTCAATATTTGATGTTTTTGTTCATTCATGTGAGTTCAGGAATCTCAAAGACGCCAGGCTTCCGAAACAAACGAAGATCTTTCAGATTGTGCAAAGAAAGGCATAGCAGGTGAGTGTTTTAAGTAGTAGTAGAAATCAATTTGATAACTTCCGTCCTATTCGTAACATTCATCCAAACTTGTCGAGATTTCTCTCTTTAACCTCCATGTTCAAAATATTCAGTAAAACCCCGGAAAGGCGACGCTCTTCTCTTCTTCAGCCTCCATCCAAATGCTATTCCAGACACAAGTAGTCTACATGGTGGGTGCCCTGTGATTGAAGGTGAGAAATGGTCAGCAACAAAGTGGATTCATGTCGATTCCTTCGACATGGTCATGAGAGACCATACAAATTGCGGTGATGAAAATCCAAGTTGTGAGAGATGGGCTGAACTCGGCGAGTGCACGAATAACCCGGAGTATATGGTGGGATCTCCTGAGCTTCCTGGCTACTGCAGGAAAAGTTGTAAGGCATGTTCATAA

mRNA sequence

ATGGCCGAATTTCTCTGTTTCAATCTACTTTTTCTCTTCACATTATCCATCTCCTTCCTTCTCCAGCGAGCTTCAGCCTCCTATGCAGGTTCCGCTAGCTCAATCGTCAACCCTGCAAAAGTCAAACAGATTTCATGGTCTCCTCGGTACTTCAATTTCTCCCTCTCCCTCTTCTTCTTCTACTACTACTACTACTACTTCCTAAGTTTTGATGATCGATTAGATTTGTTCACGGAACCCTGGTCCCGTTTCAATTTGAATGATTCTTCAGGGCTTTTGTGTATGAAGGCTAAAGCGGAGCTGAAGAGATCTTCTGTTGCGGATAATTTGTCCGGAAAGAGCAAAGTCAGCGAGGTTCGAACTAGCTCTGGGGCGTTTATTCATAAAGCCAAGGACCCTATTGTTTCTGGTATAGAAGACAAAATTGCAGCATGGACATTTCTGCCAAAAGAAAATGGCGAAGACATTCAAGTGTTGAGATATGAATATGGACAGAAGTACGATGCACACTTTGATTACTTTGCTGACAAGGTTAACATTGCCCGAGGTGGACATCGAATGGCAACCGTTCTCATGTATCTTTCCGACGTAGAAAAAGGCGGTGAAACTGTGTTTCCTTCTGCAGAGGAATCTCAAAGACGCCAGGCTTCCGAAACAAACGAAGATCTTTCAGATTGTGCAAAGAAAGGCATAGCAGTAAAACCCCGGAAAGGCGACGCTCTTCTCTTCTTCAGCCTCCATCCAAATGCTATTCCAGACACAAGTAGTCTACATGGTGGGTGCCCTGTGATTGAAGGTGAGAAATGGTCAGCAACAAAGTGGATTCATGTCGATTCCTTCGACATGGTCATGAGAGACCATACAAATTGCGGTGATGAAAATCCAAGTTGTGAGAGATGGGCTGAACTCGGCGAGTGCACGAATAACCCGGAGTATATGGTGGGATCTCCTGAGCTTCCTGGCTACTGCAGGAAAAGTTGTAAGGCATGTTCATAA

Coding sequence (CDS)

ATGGCCGAATTTCTCTGTTTCAATCTACTTTTTCTCTTCACATTATCCATCTCCTTCCTTCTCCAGCGAGCTTCAGCCTCCTATGCAGGTTCCGCTAGCTCAATCGTCAACCCTGCAAAAGTCAAACAGATTTCATGGTCTCCTCGGTACTTCAATTTCTCCCTCTCCCTCTTCTTCTTCTACTACTACTACTACTACTTCCTAAGTTTTGATGATCGATTAGATTTGTTCACGGAACCCTGGTCCCGTTTCAATTTGAATGATTCTTCAGGGCTTTTGTGTATGAAGGCTAAAGCGGAGCTGAAGAGATCTTCTGTTGCGGATAATTTGTCCGGAAAGAGCAAAGTCAGCGAGGTTCGAACTAGCTCTGGGGCGTTTATTCATAAAGCCAAGGACCCTATTGTTTCTGGTATAGAAGACAAAATTGCAGCATGGACATTTCTGCCAAAAGAAAATGGCGAAGACATTCAAGTGTTGAGATATGAATATGGACAGAAGTACGATGCACACTTTGATTACTTTGCTGACAAGGTTAACATTGCCCGAGGTGGACATCGAATGGCAACCGTTCTCATGTATCTTTCCGACGTAGAAAAAGGCGGTGAAACTGTGTTTCCTTCTGCAGAGGAATCTCAAAGACGCCAGGCTTCCGAAACAAACGAAGATCTTTCAGATTGTGCAAAGAAAGGCATAGCAGTAAAACCCCGGAAAGGCGACGCTCTTCTCTTCTTCAGCCTCCATCCAAATGCTATTCCAGACACAAGTAGTCTACATGGTGGGTGCCCTGTGATTGAAGGTGAGAAATGGTCAGCAACAAAGTGGATTCATGTCGATTCCTTCGACATGGTCATGAGAGACCATACAAATTGCGGTGATGAAAATCCAAGTTGTGAGAGATGGGCTGAACTCGGCGAGTGCACGAATAACCCGGAGTATATGGTGGGATCTCCTGAGCTTCCTGGCTACTGCAGGAAAAGTTGTAAGGCATGTTCATAA

Protein sequence

MAEFLCFNLLFLFTLSISFLLQRASASYAGSASSIVNPAKVKQISWSPRYFNFSLSLFFFYYYYYYFLSFDDRLDLFTEPWSRFNLNDSSGLLCMKAKAELKRSSVADNLSGKSKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSAEESQRRQASETNEDLSDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEKWSATKWIHVDSFDMVMRDHTNCGDENPSCERWAELGECTNNPEYMVGSPELPGYCRKSCKACS*

Homology

[Close]

BLAST of Chy6G111710 vs. ExPASy Swiss-Prot
Match: Q8LAN3 (Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=1)

HSP 1 Score: 404.1 bits (1037), Expect = 1.6e-111
Identity = 216/322 (67.08%), Postives = 235/322 (72.98%), Query Frame = 0


Query: 9   LLFLFTLSISFLLQRASASYAGSASSIVNPAKVKQISWSPRYFNFSLSLFFFYYYYYYFL 68
           LL  F    S LLQ +S S   S+S  VNP+KVKQ+S  PR         F Y  +   L
Sbjct: 6   LLISFFAIFSVLLQ-SSTSLISSSSVFVNPSKVKQVSSKPR--------AFVYEGFLTEL 65

Query: 69  SFDDRLDLFTEPWSRFNLNDSSGLLCMKAKAELKRSSVADNLSGKSKVSEVRTSSGAFIH 128
             D  + L                    AKA LKRS+VADN SG+SK SEVRTSSG FI 
Sbjct: 66  ECDHMVSL--------------------AKASLKRSAVADNDSGESKFSEVRTSSGTFIS 125

Query: 129 KAKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMA 188
           K KDPIVSGIEDKI+ WTFLPKENGEDIQVLRYE+GQKYDAHFDYF DKVNI RGGHRMA
Sbjct: 126 KGKDPIVSGIEDKISTWTFLPKENGEDIQVLRYEHGQKYDAHFDYFHDKVNIVRGGHRMA 185

Query: 189 TVLMYLSDVEKGGETVFPSAEESQRRQASETNEDLSDCAKKGIAVKPRKGDALLFFSLHP 248
           T+LMYLS+V KGGETVFP AE   RR  SE  EDLSDCAK+GIAVKPRKGDALLFF+LHP
Sbjct: 186 TILMYLSNVTKGGETVFPDAEIPSRRVLSENKEDLSDCAKRGIAVKPRKGDALLFFNLHP 245

Query: 249 NAIPDTSSLHGGCPVIEGEKWSATKWIHVDSFDMVMRDHTNCGDENPSCERWAELGECTN 308
           +AIPD  SLHGGCPVIEGEKWSATKWIHVDSFD ++    NC D N SCERWA LGECT 
Sbjct: 246 DAIPDPLSLHGGCPVIEGEKWSATKWIHVDSFDRIVTPSGNCTDMNESCERWAVLGECTK 298

Query: 309 NPEYMVGSPELPGYCRKSCKAC 331
           NPEYMVG+ ELPGYCR+SCKAC
Sbjct: 306 NPEYMVGTTELPGYCRRSCKAC 298

[Close]

BLAST of Chy6G111710 vs. ExPASy Swiss-Prot
Match: F4JAU3 (Prolyl 4-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=P4H2 PE=1 SV=1)

HSP 1 Score: 387.1 bits (993), Expect = 2.0e-106
Identity = 203/321 (63.24%), Postives = 234/321 (72.90%), Query Frame = 0


Query: 10  LFLFTLSISFLLQRASASYAGSASSIVNPAKVKQISWSPRYFNFSLSLFFFYYYYYYFLS 69
           L LF   +  LLQ +S     S SSI+NP+KVKQ+S  PR         F Y  +   L 
Sbjct: 8   LLLFVAILLVLLQ-SSTCLISSPSSIINPSKVKQVSSKPR--------AFVYEGFLTDLE 67

Query: 70  FDDRLDLFTEPWSRFNLNDSSGLLCMKAKAELKRSSVADNLSGKSKVSEVRTSSGAFIHK 129
            D  + L                    AK  L+RS+VADN +G+S+VS+VRTSSG FI K
Sbjct: 68  CDHLISL--------------------AKENLQRSAVADNDNGESQVSDVRTSSGTFISK 127

Query: 130 AKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMAT 189
            KDPIVSGIEDK++ WTFLPKENGED+QVLRYE+GQKYDAHFDYF DKVNIARGGHR+AT
Sbjct: 128 GKDPIVSGIEDKLSTWTFLPKENGEDLQVLRYEHGQKYDAHFDYFHDKVNIARGGHRIAT 187

Query: 190 VLMYLSDVEKGGETVFPSAEESQRRQASETNEDLSDCAKKGIAVKPRKGDALLFFSLHPN 249
           VL+YLS+V KGGETVFP A+E  RR  SE  +DLSDCAKKGIAVKP+KG+ALLFF+L  +
Sbjct: 188 VLLYLSNVTKGGETVFPDAQEFSRRSLSENKDDLSDCAKKGIAVKPKKGNALLFFNLQQD 247

Query: 250 AIPDTSSLHGGCPVIEGEKWSATKWIHVDSFDMVMRDHTNCGDENPSCERWAELGECTNN 309
           AIPD  SLHGGCPVIEGEKWSATKWIHVDSFD ++    NC D N SCERWA LGEC  N
Sbjct: 248 AIPDPFSLHGGCPVIEGEKWSATKWIHVDSFDKILTHDGNCTDVNESCERWAVLGECGKN 299

Query: 310 PEYMVGSPELPGYCRKSCKAC 331
           PEYMVG+PE+PG CR+SCKAC
Sbjct: 308 PEYMVGTPEIPGNCRRSCKAC 299

[Close]

BLAST of Chy6G111710 vs. ExPASy Swiss-Prot
Match: Q8L970 (Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 1.4e-88
Identity = 183/342 (53.51%), Postives = 228/342 (66.67%), Query Frame = 0


Query: 4   FLCFNLLFLFTLSI-----SFLLQRASASYAG-------SASSI-VNPAKVKQISWSPRY 63
           FL F+L FLFTL +     +  L R+S +  G       SASS   +P +V Q+SW+PR 
Sbjct: 6   FLAFSLCFLFTLPLISSAPNRFLTRSSNTRDGSVIKMKTSASSFGFDPTRVTQLSWTPRV 65

Query: 64  FNFSLSLFFFYYYYYYFLSFDDRLDLFTEPWSRFNLNDSSGLLCMKAKAELKRSSVADNL 123
           F            Y  FLS D+  D F +                 AK +L++S VADN 
Sbjct: 66  F-----------LYEGFLS-DEECDHFIK----------------LAKGKLEKSMVADND 125

Query: 124 SGKSKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAH 183
           SG+S  SEVRTSSG F+ K +D IVS +E K+AAWTFLP+ENGE +Q+L YE GQKY+ H
Sbjct: 126 SGESVESEVRTSSGMFLSKRQDDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPH 185

Query: 184 FDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSAEESQRRQASETNED-LSDCAKK 243
           FDYF D+ N+  GGHR+ATVLMYLS+VEKGGETVFP      + +A++  +D  ++CAK+
Sbjct: 186 FDYFHDQANLELGGHRIATVLMYLSNVEKGGETVFP----MWKGKATQLKDDSWTECAKQ 245

Query: 244 GIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEKWSATKWIHVDSFDMVMRDHTN 303
           G AVKPRKGDALLFF+LHPNA  D++SLHG CPV+EGEKWSAT+WIHV SF+      + 
Sbjct: 246 GYAVKPRKGDALLFFNLHPNATTDSNSLHGSCPVVEGEKWSATRWIHVKSFERAFNKQSG 305

Query: 304 CGDENPSCERWAELGECTNNPEYMVGSPELPGYCRKSCKACS 332
           C DEN SCE+WA+ GEC  NP YMVGS +  GYCRKSCKACS
Sbjct: 306 CMDENVSCEKWAKAGECQKNPTYMVGSDKDHGYCRKSCKACS 315

[Close]

BLAST of Chy6G111710 vs. ExPASy Swiss-Prot
Match: F4J0A8 (Probable prolyl 4-hydroxylase 6 OS=Arabidopsis thaliana OX=3702 GN=P4H6 PE=2 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 8.1e-76
Identity = 164/321 (51.09%), Postives = 203/321 (63.24%), Query Frame = 0


Query: 11  FLFTLSISFLLQRASASYAGSASSIVNPAKVKQISWSPRYFNFSLSLFFFYYYYYYFLSF 70
           +    S+S LL     S   S S  V+P ++ Q+SW+PR F            Y  FLS 
Sbjct: 5   YFLAFSLSLLL---IFSQISSFSFSVDPTRITQLSWTPRAF-----------LYKGFLS- 64

Query: 71  DDRLDLFTEPWSRFNLNDSSGLLCMKAKAELKRSS-VADNLSGKSKVSEVRTSSGAFIHK 130
           D+  D                 L   AK +L++S  VAD  SG+S+ SEVRTSSG F+ K
Sbjct: 65  DEECD----------------HLIKLAKGKLEKSMVVADVDSGESEDSEVRTSSGMFLTK 124

Query: 131 AKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMAT 190
            +D IV+ +E K+AAWTFLP+ENGE +Q+L YE GQKYD HFDYF DK  +  GGHR+AT
Sbjct: 125 RQDDIVANVEAKLAAWTFLPEENGEALQILHYENGQKYDPHFDYFYDKKALELGGHRIAT 184

Query: 191 VLMYLSDVEKGGETVFPSAEESQRRQASETNEDLSDCAKKGIAVKPRKGDALLFFSLHPN 250
           VLMYLS+V KGGETVFP+    + +     ++  S CAK+G AVKPRKGDALLFF+LH N
Sbjct: 185 VLMYLSNVTKGGETVFPN---WKGKTPQLKDDSWSKCAKQGYAVKPRKGDALLFFNLHLN 244

Query: 251 AIPDTSSLHGGCPVIEGEKWSATKWIHVDSFDMVMRDHTNCGDENPSCERWAELGECTNN 310
              D +SLHG CPVIEGEKWSAT+WIHV SF    +    C D++ SC+ WA+ GEC  N
Sbjct: 245 GTTDPNSLHGSCPVIEGEKWSATRWIHVRSFG---KKKLVCVDDHESCQEWADAGECEKN 288

Query: 311 PEYMVGSPELPGYCRKSCKAC 331
           P YMVGS    G+CRKSCKAC
Sbjct: 305 PMYMVGSETSLGFCRKSCKAC 288

[Close]

BLAST of Chy6G111710 vs. ExPASy Swiss-Prot
Match: Q9LN20 (Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 1.8e-54
Identity = 113/237 (47.68%), Postives = 150/237 (63.29%), Query Frame = 0


Query: 44  ISWSPRYFNFSLSLFFFYYYYYYFLSFDDRLDLFTEPWSRFNLNDSSGLLCMKAKAELKR 103
           +SW PR F            Y+ FLS                  +    L   AK  + +
Sbjct: 79  LSWEPRAF-----------VYHNFLS-----------------KEECEYLISLAKPHMVK 138

Query: 104 SSVADNLSGKSKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEY 163
           S+V D+ +GKSK S VRTSSG F+ + +D I+  IE +IA +TF+P ++GE +QVL YE 
Sbjct: 139 STVVDSETGKSKDSRVRTSSGTFLRRGRDKIIKTIEKRIADYTFIPADHGEGLQVLHYEA 198

Query: 164 GQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSAEESQRRQASETNEDL 223
           GQKY+ H+DYF D+ N   GG RMAT+LMYLSDVE+GGETVFP+A  +    +     +L
Sbjct: 199 GQKYEPHYDYFVDEFNTKNGGQRMATMLMYLSDVEEGGETVFPAA--NMNFSSVPWYNEL 258

Query: 224 SDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEKWSATKWIHVDSF 281
           S+C KKG++VKPR GDALLF+S+ P+A  D +SLHGGCPVI G KWS+TKW+HV  +
Sbjct: 259 SECGKKGLSVKPRMGDALLFWSMRPDATLDPTSLHGGCPVIRGNKWSSTKWMHVGEY 285

[Close]

BLAST of Chy6G111710 vs. ExPASy TrEMBL
Match: A0A0A0L5Q6 (Procollagen-proline 4-dioxygenase OS=Cucumis sativus OX=3659 GN=Csa_3G150820 PE=3 SV=1)

HSP 1 Score: 550.1 bits (1416), Expect = 6.5e-153
Identity = 285/331 (86.10%), Postives = 289/331 (87.31%), Query Frame = 0


Query: 1   MAEFLCFNLLFLFTLSISFLLQRASASYAGSASSIVNPAKVKQISWSPRYFNFSLSLFFF 60
           MAE LCFNLLFLFTLSISFLLQRASASYAGSASSIVNPAKVKQISWSPR         F 
Sbjct: 37  MAELLCFNLLFLFTLSISFLLQRASASYAGSASSIVNPAKVKQISWSPR--------AFV 96

Query: 61  YYYYYYFLSFDDRLDLFTEPWSRFNLNDSSGLLCMKAKAELKRSSVADNLSGKSKVSEVR 120
           Y  +   L  D  + L                    AKAELKRSSVADNLSGKSKVSEVR
Sbjct: 97  YEGFLTDLECDHLISL--------------------AKAELKRSSVADNLSGKSKVSEVR 156

Query: 121 TSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFADKVNI 180
           TSSGAFIHKAKDPIVSGIEDKIAAWTFLPK+NGEDIQVLRYEYGQKYDAHFDYFADKVNI
Sbjct: 157 TSSGAFIHKAKDPIVSGIEDKIAAWTFLPKDNGEDIQVLRYEYGQKYDAHFDYFADKVNI 216

Query: 181 ARGGHRMATVLMYLSDVEKGGETVFPSAEESQRRQASETNEDLSDCAKKGIAVKPRKGDA 240
           ARGGHRMATVLMYLSDVEKGGETVFPSAEESQRRQASETNEDLSDCAKKGIAVKPRKGDA
Sbjct: 217 ARGGHRMATVLMYLSDVEKGGETVFPSAEESQRRQASETNEDLSDCAKKGIAVKPRKGDA 276

Query: 241 LLFFSLHPNAIPDTSSLHGGCPVIEGEKWSATKWIHVDSFDMVMRDHTNCGDENPSCERW 300
           LLFFSLHPNAIPDTSSLHGGCPVIEGEKWSATKWI VDSFDMV+RDHTNCGDENPSCERW
Sbjct: 277 LLFFSLHPNAIPDTSSLHGGCPVIEGEKWSATKWIRVDSFDMVVRDHTNCGDENPSCERW 336

Query: 301 AELGECTNNPEYMVGSPELPGYCRKSCKACS 332
           AELGECTNNPEYMVGSPELPGYCRKSCKACS
Sbjct: 337 AELGECTNNPEYMVGSPELPGYCRKSCKACS 339

[Close]

BLAST of Chy6G111710 vs. ExPASy TrEMBL
Match: A0A1S3AWU7 (Procollagen-proline 4-dioxygenase OS=Cucumis melo OX=3656 GN=LOC103483779 PE=3 SV=1)

HSP 1 Score: 542.0 bits (1395), Expect = 1.8e-150
Identity = 280/331 (84.59%), Postives = 288/331 (87.01%), Query Frame = 0


Query: 1   MAEFLCFNLLFLFTLSISFLLQRASASYAGSASSIVNPAKVKQISWSPRYFNFSLSLFFF 60
           MAEFL FNLLFLFTL+IS+LL+RASASYAGSASSIVNPAKVKQISWSPR         F 
Sbjct: 1   MAEFLRFNLLFLFTLTISYLLRRASASYAGSASSIVNPAKVKQISWSPR--------AFV 60

Query: 61  YYYYYYFLSFDDRLDLFTEPWSRFNLNDSSGLLCMKAKAELKRSSVADNLSGKSKVSEVR 120
           Y  +   L  D  + L                    AKAELKRSSVADNLSG+SKVSEVR
Sbjct: 61  YEGFLTDLECDHLISL--------------------AKAELKRSSVADNLSGESKVSEVR 120

Query: 121 TSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFADKVNI 180
           TSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFADKVNI
Sbjct: 121 TSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFADKVNI 180

Query: 181 ARGGHRMATVLMYLSDVEKGGETVFPSAEESQRRQASETNEDLSDCAKKGIAVKPRKGDA 240
           ARGGHRMATVLMYLSDVEKGGETVFPSAEESQRRQASETN+DLSDCAKKGIAVKPRKGDA
Sbjct: 181 ARGGHRMATVLMYLSDVEKGGETVFPSAEESQRRQASETNQDLSDCAKKGIAVKPRKGDA 240

Query: 241 LLFFSLHPNAIPDTSSLHGGCPVIEGEKWSATKWIHVDSFDMVMRDHTNCGDENPSCERW 300
           LLFFSLHPNAIPDTSSLHGGCPVIEGEKWSATKWIHVDSFD + RDHTNCGDENPSCERW
Sbjct: 241 LLFFSLHPNAIPDTSSLHGGCPVIEGEKWSATKWIHVDSFDTIARDHTNCGDENPSCERW 300

Query: 301 AELGECTNNPEYMVGSPELPGYCRKSCKACS 332
           AELGECTNNPEYMVGSPELPGYCRKSCKACS
Sbjct: 301 AELGECTNNPEYMVGSPELPGYCRKSCKACS 303

[Close]

BLAST of Chy6G111710 vs. ExPASy TrEMBL
Match: A0A5A7U593 (Procollagen-proline 4-dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G00280 PE=3 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 1.3e-148
Identity = 277/331 (83.69%), Postives = 286/331 (86.40%), Query Frame = 0


Query: 1   MAEFLCFNLLFLFTLSISFLLQRASASYAGSASSIVNPAKVKQISWSPRYFNFSLSLFFF 60
           MAEFL FNLLFLFTL+IS+LL+RASASYAGSASSIVNPAKVKQISW+           F 
Sbjct: 1   MAEFLRFNLLFLFTLTISYLLRRASASYAGSASSIVNPAKVKQISWA-----------FV 60

Query: 61  YYYYYYFLSFDDRLDLFTEPWSRFNLNDSSGLLCMKAKAELKRSSVADNLSGKSKVSEVR 120
           Y  +   L  D  + L                    AKAELKRSSVADNLSG+SKVSEVR
Sbjct: 61  YEGFLTDLECDHLISL--------------------AKAELKRSSVADNLSGESKVSEVR 120

Query: 121 TSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFADKVNI 180
           TSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFADKVNI
Sbjct: 121 TSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFADKVNI 180

Query: 181 ARGGHRMATVLMYLSDVEKGGETVFPSAEESQRRQASETNEDLSDCAKKGIAVKPRKGDA 240
           ARGGHRMATVLMYLSDVEKGGETVFPSAEESQRRQASETN+DLSDCAKKGIAVKPRKGDA
Sbjct: 181 ARGGHRMATVLMYLSDVEKGGETVFPSAEESQRRQASETNQDLSDCAKKGIAVKPRKGDA 240

Query: 241 LLFFSLHPNAIPDTSSLHGGCPVIEGEKWSATKWIHVDSFDMVMRDHTNCGDENPSCERW 300
           LLFFSLHPNAIPDTSSLHGGCPVIEGEKWSATKWIHVDSFD + RDHTNCGDENPSCERW
Sbjct: 241 LLFFSLHPNAIPDTSSLHGGCPVIEGEKWSATKWIHVDSFDTIARDHTNCGDENPSCERW 300

Query: 301 AELGECTNNPEYMVGSPELPGYCRKSCKACS 332
           AELGECTNNPEYMVGSPELPGYCRKSCKACS
Sbjct: 301 AELGECTNNPEYMVGSPELPGYCRKSCKACS 300

[Close]

BLAST of Chy6G111710 vs. ExPASy TrEMBL
Match: A0A6J1C7M6 (Procollagen-proline 4-dioxygenase OS=Momordica charantia OX=3673 GN=LOC111009125 PE=3 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 8.9e-142
Identity = 263/330 (79.70%), Postives = 279/330 (84.55%), Query Frame = 0


Query: 1   MAEFLCFNLLFLFTLSISFLLQRASASYAGSASSIVNPAKVKQISWSPRYFNFSLSLFFF 60
           MA F   +LLF F+LSIS LL+RAS+SYAGSASSIVNPAKVKQISWSPR         F 
Sbjct: 1   MARFCSCSLLFFFSLSISLLLRRASSSYAGSASSIVNPAKVKQISWSPR--------AFV 60

Query: 61  YYYYYYFLSFDDRLDLFTEPWSRFNLNDSSGLLCMKAKAELKRSSVADNLSGKSKVSEVR 120
           Y  +   L  D  + L                    AKAELKRS+VADNLSG+SKVSEVR
Sbjct: 61  YEGFLTDLECDHIISL--------------------AKAELKRSAVADNLSGESKVSEVR 120

Query: 121 TSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFADKVNI 180
           TSSGAFIHKAKDPI+SGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYF+DKVNI
Sbjct: 121 TSSGAFIHKAKDPIISGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFSDKVNI 180

Query: 181 ARGGHRMATVLMYLSDVEKGGETVFPSAEESQRRQASETNEDLSDCAKKGIAVKPRKGDA 240
           ARGGHRMATVLMYLS+VEKGGETVFPSAEESQRRQASETNEDLSDCAKKGIAVKPR+GDA
Sbjct: 181 ARGGHRMATVLMYLSNVEKGGETVFPSAEESQRRQASETNEDLSDCAKKGIAVKPRRGDA 240

Query: 241 LLFFSLHPNAIPDTSSLHGGCPVIEGEKWSATKWIHVDSFDMVMRDHTNCGDENPSCERW 300
           LLFFSLHPNA+PDT SLHGGCPVIEGEKWSATKWIHVDSFD ++RDHTNC DE+ SCERW
Sbjct: 241 LLFFSLHPNAVPDTCSLHGGCPVIEGEKWSATKWIHVDSFDTILRDHTNCADEHASCERW 300

Query: 301 AELGECTNNPEYMVGSPELPGYCRKSCKAC 331
           AELGECTNNPEYMVGSPELPGYCRKSCK C
Sbjct: 301 AELGECTNNPEYMVGSPELPGYCRKSCKVC 302

[Close]

BLAST of Chy6G111710 vs. ExPASy TrEMBL
Match: A0A6J1I971 (Procollagen-proline 4-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111472232 PE=3 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 1.1e-139
Identity = 260/330 (78.79%), Postives = 277/330 (83.94%), Query Frame = 0


Query: 1   MAEFLCFNLLFLFTLSISFLLQRASASYAGSASSIVNPAKVKQISWSPRYFNFSLSLFFF 60
           MA+F   NLLF+ ++SIS LL+RAS+SYAGSASSIVNPAKVKQISWSPR         F 
Sbjct: 1   MAKFCSCNLLFILSISISLLLRRASSSYAGSASSIVNPAKVKQISWSPR--------AFV 60

Query: 61  YYYYYYFLSFDDRLDLFTEPWSRFNLNDSSGLLCMKAKAELKRSSVADNLSGKSKVSEVR 120
           Y  +   L  D  + L                    AKAELKRS+VAD+LSG+SKVSEVR
Sbjct: 61  YEGFLTDLECDHLISL--------------------AKAELKRSAVADHLSGESKVSEVR 120

Query: 121 TSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFADKVNI 180
           TSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYF DKVNI
Sbjct: 121 TSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFTDKVNI 180

Query: 181 ARGGHRMATVLMYLSDVEKGGETVFPSAEESQRRQASETNEDLSDCAKKGIAVKPRKGDA 240
           ARGGHRMATVLMYLS+VEKGGETVFP+AEESQRRQASETNEDLSDCAKKGIAVKPRKGDA
Sbjct: 181 ARGGHRMATVLMYLSNVEKGGETVFPNAEESQRRQASETNEDLSDCAKKGIAVKPRKGDA 240

Query: 241 LLFFSLHPNAIPDTSSLHGGCPVIEGEKWSATKWIHVDSFDMVMRDHTNCGDENPSCERW 300
           LLFFSLHPNA+PDT SLHGGCPVIEGEKWSATKWIHVDSFD ++ DHT+C D N SCERW
Sbjct: 241 LLFFSLHPNAVPDTKSLHGGCPVIEGEKWSATKWIHVDSFDTIVSDHTSCVDNNASCERW 300

Query: 301 AELGECTNNPEYMVGSPELPGYCRKSCKAC 331
           AELGECTNNPEYMVGSPELPGYCRKSCK C
Sbjct: 301 AELGECTNNPEYMVGSPELPGYCRKSCKVC 302

[Close]

BLAST of Chy6G111710 vs. NCBI nr
Match: XP_004134175.2 (probable prolyl 4-hydroxylase 4 [Cucumis sativus] >KGN57053.1 hypothetical protein Csa_010114 [Cucumis sativus])

HSP 1 Score: 549 bits (1415), Expect = 4.30e-195
Identity = 285/331 (86.10%), Postives = 289/331 (87.31%), Query Frame = 0


Query: 1   MAEFLCFNLLFLFTLSISFLLQRASASYAGSASSIVNPAKVKQISWSPRYFNFSLSLFFF 60
           MAE LCFNLLFLFTLSISFLLQRASASYAGSASSIVNPAKVKQISWSPR F +   L   
Sbjct: 37  MAELLCFNLLFLFTLSISFLLQRASASYAGSASSIVNPAKVKQISWSPRAFVYEGFL--- 96

Query: 61  YYYYYYFLSFDDRLDLFTEPWSRFNLNDSSGLLCMKAKAELKRSSVADNLSGKSKVSEVR 120
                         DL  +             L   AKAELKRSSVADNLSGKSKVSEVR
Sbjct: 97  -------------TDLECDH------------LISLAKAELKRSSVADNLSGKSKVSEVR 156

Query: 121 TSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFADKVNI 180
           TSSGAFIHKAKDPIVSGIEDKIAAWTFLPK+NGEDIQVLRYEYGQKYDAHFDYFADKVNI
Sbjct: 157 TSSGAFIHKAKDPIVSGIEDKIAAWTFLPKDNGEDIQVLRYEYGQKYDAHFDYFADKVNI 216

Query: 181 ARGGHRMATVLMYLSDVEKGGETVFPSAEESQRRQASETNEDLSDCAKKGIAVKPRKGDA 240
           ARGGHRMATVLMYLSDVEKGGETVFPSAEESQRRQASETNEDLSDCAKKGIAVKPRKGDA
Sbjct: 217 ARGGHRMATVLMYLSDVEKGGETVFPSAEESQRRQASETNEDLSDCAKKGIAVKPRKGDA 276

Query: 241 LLFFSLHPNAIPDTSSLHGGCPVIEGEKWSATKWIHVDSFDMVMRDHTNCGDENPSCERW 300
           LLFFSLHPNAIPDTSSLHGGCPVIEGEKWSATKWI VDSFDMV+RDHTNCGDENPSCERW
Sbjct: 277 LLFFSLHPNAIPDTSSLHGGCPVIEGEKWSATKWIRVDSFDMVVRDHTNCGDENPSCERW 336

Query: 301 AELGECTNNPEYMVGSPELPGYCRKSCKACS 331
           AELGECTNNPEYMVGSPELPGYCRKSCKACS
Sbjct: 337 AELGECTNNPEYMVGSPELPGYCRKSCKACS 339

[Close]

BLAST of Chy6G111710 vs. NCBI nr
Match: XP_008438765.1 (PREDICTED: probable prolyl 4-hydroxylase 4 [Cucumis melo])

HSP 1 Score: 541 bits (1394), Expect = 1.81e-192
Identity = 280/331 (84.59%), Postives = 288/331 (87.01%), Query Frame = 0


Query: 1   MAEFLCFNLLFLFTLSISFLLQRASASYAGSASSIVNPAKVKQISWSPRYFNFSLSLFFF 60
           MAEFL FNLLFLFTL+IS+LL+RASASYAGSASSIVNPAKVKQISWSPR F +   L   
Sbjct: 1   MAEFLRFNLLFLFTLTISYLLRRASASYAGSASSIVNPAKVKQISWSPRAFVYEGFL--- 60

Query: 61  YYYYYYFLSFDDRLDLFTEPWSRFNLNDSSGLLCMKAKAELKRSSVADNLSGKSKVSEVR 120
                         DL  +             L   AKAELKRSSVADNLSG+SKVSEVR
Sbjct: 61  -------------TDLECDH------------LISLAKAELKRSSVADNLSGESKVSEVR 120

Query: 121 TSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFADKVNI 180
           TSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFADKVNI
Sbjct: 121 TSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFADKVNI 180

Query: 181 ARGGHRMATVLMYLSDVEKGGETVFPSAEESQRRQASETNEDLSDCAKKGIAVKPRKGDA 240
           ARGGHRMATVLMYLSDVEKGGETVFPSAEESQRRQASETN+DLSDCAKKGIAVKPRKGDA
Sbjct: 181 ARGGHRMATVLMYLSDVEKGGETVFPSAEESQRRQASETNQDLSDCAKKGIAVKPRKGDA 240

Query: 241 LLFFSLHPNAIPDTSSLHGGCPVIEGEKWSATKWIHVDSFDMVMRDHTNCGDENPSCERW 300
           LLFFSLHPNAIPDTSSLHGGCPVIEGEKWSATKWIHVDSFD + RDHTNCGDENPSCERW
Sbjct: 241 LLFFSLHPNAIPDTSSLHGGCPVIEGEKWSATKWIHVDSFDTIARDHTNCGDENPSCERW 300

Query: 301 AELGECTNNPEYMVGSPELPGYCRKSCKACS 331
           AELGECTNNPEYMVGSPELPGYCRKSCKACS
Sbjct: 301 AELGECTNNPEYMVGSPELPGYCRKSCKACS 303

[Close]

BLAST of Chy6G111710 vs. NCBI nr
Match: KAA0049426.1 (putative prolyl 4-hydroxylase 4 [Cucumis melo var. makuwa] >TYK16104.1 putative prolyl 4-hydroxylase 4 [Cucumis melo var. makuwa])

HSP 1 Score: 535 bits (1378), Expect = 4.44e-190
Identity = 277/331 (83.69%), Postives = 285/331 (86.10%), Query Frame = 0


Query: 1   MAEFLCFNLLFLFTLSISFLLQRASASYAGSASSIVNPAKVKQISWSPRYFNFSLSLFFF 60
           MAEFL FNLLFLFTL+IS+LL+RASASYAGSASSIVNPAKVKQISW+  Y  F   L   
Sbjct: 1   MAEFLRFNLLFLFTLTISYLLRRASASYAGSASSIVNPAKVKQISWAFVYEGFLTDL--- 60

Query: 61  YYYYYYFLSFDDRLDLFTEPWSRFNLNDSSGLLCMKAKAELKRSSVADNLSGKSKVSEVR 120
                     D  + L                    AKAELKRSSVADNLSG+SKVSEVR
Sbjct: 61  --------ECDHLISL--------------------AKAELKRSSVADNLSGESKVSEVR 120

Query: 121 TSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFADKVNI 180
           TSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFADKVNI
Sbjct: 121 TSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFADKVNI 180

Query: 181 ARGGHRMATVLMYLSDVEKGGETVFPSAEESQRRQASETNEDLSDCAKKGIAVKPRKGDA 240
           ARGGHRMATVLMYLSDVEKGGETVFPSAEESQRRQASETN+DLSDCAKKGIAVKPRKGDA
Sbjct: 181 ARGGHRMATVLMYLSDVEKGGETVFPSAEESQRRQASETNQDLSDCAKKGIAVKPRKGDA 240

Query: 241 LLFFSLHPNAIPDTSSLHGGCPVIEGEKWSATKWIHVDSFDMVMRDHTNCGDENPSCERW 300
           LLFFSLHPNAIPDTSSLHGGCPVIEGEKWSATKWIHVDSFD + RDHTNCGDENPSCERW
Sbjct: 241 LLFFSLHPNAIPDTSSLHGGCPVIEGEKWSATKWIHVDSFDTIARDHTNCGDENPSCERW 300

Query: 301 AELGECTNNPEYMVGSPELPGYCRKSCKACS 331
           AELGECTNNPEYMVGSPELPGYCRKSCKACS
Sbjct: 301 AELGECTNNPEYMVGSPELPGYCRKSCKACS 300

[Close]

BLAST of Chy6G111710 vs. NCBI nr
Match: XP_038903083.1 (probable prolyl 4-hydroxylase 4 [Benincasa hispida])

HSP 1 Score: 527 bits (1357), Expect = 7.84e-187
Identity = 271/331 (81.87%), Postives = 284/331 (85.80%), Query Frame = 0


Query: 1   MAEFLCFNLLFLFTLSISFLLQRASASYAGSASSIVNPAKVKQISWSPRYFNFSLSLFFF 60
           MA+F CFNLLF F+LSIS LL+RAS+SYAGSASSIVNPAKVKQISW+PR F +   L   
Sbjct: 1   MAKFYCFNLLFFFSLSISCLLRRASSSYAGSASSIVNPAKVKQISWNPRAFVYEGFL--- 60

Query: 61  YYYYYYFLSFDDRLDLFTEPWSRFNLNDSSGLLCMKAKAELKRSSVADNLSGKSKVSEVR 120
                         DL  +             L   AKAELKRSSVADNLSG+SKVSEVR
Sbjct: 61  -------------TDLECDH------------LISLAKAELKRSSVADNLSGESKVSEVR 120

Query: 121 TSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFADKVNI 180
           TSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFADKVNI
Sbjct: 121 TSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFADKVNI 180

Query: 181 ARGGHRMATVLMYLSDVEKGGETVFPSAEESQRRQASETNEDLSDCAKKGIAVKPRKGDA 240
           ARGGHRMATVLMYLSDVEKGGETVFPSAEESQRRQASETN+DLSDCAKKGIAVKPRKGDA
Sbjct: 181 ARGGHRMATVLMYLSDVEKGGETVFPSAEESQRRQASETNDDLSDCAKKGIAVKPRKGDA 240

Query: 241 LLFFSLHPNAIPDTSSLHGGCPVIEGEKWSATKWIHVDSFDMVMRDHTNCGDENPSCERW 300
           LLFFSLHPNAIPD SSLHGGCPVIEGEKWSATKWIHVDSFD ++RDHT+C DENPSCERW
Sbjct: 241 LLFFSLHPNAIPDISSLHGGCPVIEGEKWSATKWIHVDSFDKIIRDHTDCADENPSCERW 300

Query: 301 AELGECTNNPEYMVGSPELPGYCRKSCKACS 331
           AEL ECTNNPEYMVGSPELPGYCRKSCKAC+
Sbjct: 301 AELSECTNNPEYMVGSPELPGYCRKSCKACA 303

[Close]

BLAST of Chy6G111710 vs. NCBI nr
Match: XP_022137761.1 (probable prolyl 4-hydroxylase 4 [Momordica charantia])

HSP 1 Score: 512 bits (1318), Expect = 6.58e-181
Identity = 262/330 (79.39%), Postives = 279/330 (84.55%), Query Frame = 0


Query: 1   MAEFLCFNLLFLFTLSISFLLQRASASYAGSASSIVNPAKVKQISWSPRYFNFSLSLFFF 60
           MA F   +LLF F+LSIS LL+RAS+SYAGSASSIVNPAKVKQISWSPR F +   L   
Sbjct: 1   MARFCSCSLLFFFSLSISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFL--- 60

Query: 61  YYYYYYFLSFDDRLDLFTEPWSRFNLNDSSGLLCMKAKAELKRSSVADNLSGKSKVSEVR 120
                         DL  +             +   AKAELKRS+VADNLSG+SKVSEVR
Sbjct: 61  -------------TDLECDH------------IISLAKAELKRSAVADNLSGESKVSEVR 120

Query: 121 TSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFADKVNI 180
           TSSGAFIHKAKDPI+SGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYF+DKVNI
Sbjct: 121 TSSGAFIHKAKDPIISGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFSDKVNI 180

Query: 181 ARGGHRMATVLMYLSDVEKGGETVFPSAEESQRRQASETNEDLSDCAKKGIAVKPRKGDA 240
           ARGGHRMATVLMYLS+VEKGGETVFPSAEESQRRQASETNEDLSDCAKKGIAVKPR+GDA
Sbjct: 181 ARGGHRMATVLMYLSNVEKGGETVFPSAEESQRRQASETNEDLSDCAKKGIAVKPRRGDA 240

Query: 241 LLFFSLHPNAIPDTSSLHGGCPVIEGEKWSATKWIHVDSFDMVMRDHTNCGDENPSCERW 300
           LLFFSLHPNA+PDT SLHGGCPVIEGEKWSATKWIHVDSFD ++RDHTNC DE+ SCERW
Sbjct: 241 LLFFSLHPNAVPDTCSLHGGCPVIEGEKWSATKWIHVDSFDTILRDHTNCADEHASCERW 300

Query: 301 AELGECTNNPEYMVGSPELPGYCRKSCKAC 330
           AELGECTNNPEYMVGSPELPGYCRKSCK C
Sbjct: 301 AELGECTNNPEYMVGSPELPGYCRKSCKVC 302

[Close]

BLAST of Chy6G111710 vs. TAIR 10
Match: AT5G18900.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 404.1 bits (1037), Expect = 1.1e-112
Identity = 216/322 (67.08%), Postives = 235/322 (72.98%), Query Frame = 0


Query: 9   LLFLFTLSISFLLQRASASYAGSASSIVNPAKVKQISWSPRYFNFSLSLFFFYYYYYYFL 68
           LL  F    S LLQ +S S   S+S  VNP+KVKQ+S  PR         F Y  +   L
Sbjct: 6   LLISFFAIFSVLLQ-SSTSLISSSSVFVNPSKVKQVSSKPR--------AFVYEGFLTEL 65

Query: 69  SFDDRLDLFTEPWSRFNLNDSSGLLCMKAKAELKRSSVADNLSGKSKVSEVRTSSGAFIH 128
             D  + L                    AKA LKRS+VADN SG+SK SEVRTSSG FI 
Sbjct: 66  ECDHMVSL--------------------AKASLKRSAVADNDSGESKFSEVRTSSGTFIS 125

Query: 129 KAKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMA 188
           K KDPIVSGIEDKI+ WTFLPKENGEDIQVLRYE+GQKYDAHFDYF DKVNI RGGHRMA
Sbjct: 126 KGKDPIVSGIEDKISTWTFLPKENGEDIQVLRYEHGQKYDAHFDYFHDKVNIVRGGHRMA 185

Query: 189 TVLMYLSDVEKGGETVFPSAEESQRRQASETNEDLSDCAKKGIAVKPRKGDALLFFSLHP 248
           T+LMYLS+V KGGETVFP AE   RR  SE  EDLSDCAK+GIAVKPRKGDALLFF+LHP
Sbjct: 186 TILMYLSNVTKGGETVFPDAEIPSRRVLSENKEDLSDCAKRGIAVKPRKGDALLFFNLHP 245

Query: 249 NAIPDTSSLHGGCPVIEGEKWSATKWIHVDSFDMVMRDHTNCGDENPSCERWAELGECTN 308
           +AIPD  SLHGGCPVIEGEKWSATKWIHVDSFD ++    NC D N SCERWA LGECT 
Sbjct: 246 DAIPDPLSLHGGCPVIEGEKWSATKWIHVDSFDRIVTPSGNCTDMNESCERWAVLGECTK 298

Query: 309 NPEYMVGSPELPGYCRKSCKAC 331
           NPEYMVG+ ELPGYCR+SCKAC
Sbjct: 306 NPEYMVGTTELPGYCRRSCKAC 298

[Close]

BLAST of Chy6G111710 vs. TAIR 10
Match: AT3G06300.1 (P4H isoform 2 )

HSP 1 Score: 387.1 bits (993), Expect = 1.4e-107
Identity = 203/321 (63.24%), Postives = 234/321 (72.90%), Query Frame = 0


Query: 10  LFLFTLSISFLLQRASASYAGSASSIVNPAKVKQISWSPRYFNFSLSLFFFYYYYYYFLS 69
           L LF   +  LLQ +S     S SSI+NP+KVKQ+S  PR         F Y  +   L 
Sbjct: 8   LLLFVAILLVLLQ-SSTCLISSPSSIINPSKVKQVSSKPR--------AFVYEGFLTDLE 67

Query: 70  FDDRLDLFTEPWSRFNLNDSSGLLCMKAKAELKRSSVADNLSGKSKVSEVRTSSGAFIHK 129
            D  + L                    AK  L+RS+VADN +G+S+VS+VRTSSG FI K
Sbjct: 68  CDHLISL--------------------AKENLQRSAVADNDNGESQVSDVRTSSGTFISK 127

Query: 130 AKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMAT 189
            KDPIVSGIEDK++ WTFLPKENGED+QVLRYE+GQKYDAHFDYF DKVNIARGGHR+AT
Sbjct: 128 GKDPIVSGIEDKLSTWTFLPKENGEDLQVLRYEHGQKYDAHFDYFHDKVNIARGGHRIAT 187

Query: 190 VLMYLSDVEKGGETVFPSAEESQRRQASETNEDLSDCAKKGIAVKPRKGDALLFFSLHPN 249
           VL+YLS+V KGGETVFP A+E  RR  SE  +DLSDCAKKGIAVKP+KG+ALLFF+L  +
Sbjct: 188 VLLYLSNVTKGGETVFPDAQEFSRRSLSENKDDLSDCAKKGIAVKPKKGNALLFFNLQQD 247

Query: 250 AIPDTSSLHGGCPVIEGEKWSATKWIHVDSFDMVMRDHTNCGDENPSCERWAELGECTNN 309
           AIPD  SLHGGCPVIEGEKWSATKWIHVDSFD ++    NC D N SCERWA LGEC  N
Sbjct: 248 AIPDPFSLHGGCPVIEGEKWSATKWIHVDSFDKILTHDGNCTDVNESCERWAVLGECGKN 299

Query: 310 PEYMVGSPELPGYCRKSCKAC 331
           PEYMVG+PE+PG CR+SCKAC
Sbjct: 308 PEYMVGTPEIPGNCRRSCKAC 299

[Close]

BLAST of Chy6G111710 vs. TAIR 10
Match: AT3G28480.1 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 327.8 bits (839), Expect = 1.0e-89
Identity = 183/342 (53.51%), Postives = 228/342 (66.67%), Query Frame = 0


Query: 4   FLCFNLLFLFTLSI-----SFLLQRASASYAG-------SASSI-VNPAKVKQISWSPRY 63
           FL F+L FLFTL +     +  L R+S +  G       SASS   +P +V Q+SW+PR 
Sbjct: 6   FLAFSLCFLFTLPLISSAPNRFLTRSSNTRDGSVIKMKTSASSFGFDPTRVTQLSWTPRV 65

Query: 64  FNFSLSLFFFYYYYYYFLSFDDRLDLFTEPWSRFNLNDSSGLLCMKAKAELKRSSVADNL 123
           F            Y  FLS D+  D F +                 AK +L++S VADN 
Sbjct: 66  F-----------LYEGFLS-DEECDHFIK----------------LAKGKLEKSMVADND 125

Query: 124 SGKSKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAH 183
           SG+S  SEVRTSSG F+ K +D IVS +E K+AAWTFLP+ENGE +Q+L YE GQKY+ H
Sbjct: 126 SGESVESEVRTSSGMFLSKRQDDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPH 185

Query: 184 FDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSAEESQRRQASETNED-LSDCAKK 243
           FDYF D+ N+  GGHR+ATVLMYLS+VEKGGETVFP      + +A++  +D  ++CAK+
Sbjct: 186 FDYFHDQANLELGGHRIATVLMYLSNVEKGGETVFP----MWKGKATQLKDDSWTECAKQ 245

Query: 244 GIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEKWSATKWIHVDSFDMVMRDHTN 303
           G AVKPRKGDALLFF+LHPNA  D++SLHG CPV+EGEKWSAT+WIHV SF+      + 
Sbjct: 246 GYAVKPRKGDALLFFNLHPNATTDSNSLHGSCPVVEGEKWSATRWIHVKSFERAFNKQSG 305

Query: 304 CGDENPSCERWAELGECTNNPEYMVGSPELPGYCRKSCKACS 332
           C DEN SCE+WA+ GEC  NP YMVGS +  GYCRKSCKACS
Sbjct: 306 CMDENVSCEKWAKAGECQKNPTYMVGSDKDHGYCRKSCKACS 315

[Close]

BLAST of Chy6G111710 vs. TAIR 10
Match: AT3G28480.2 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 305.8 bits (782), Expect = 4.1e-83
Identity = 179/350 (51.14%), Postives = 223/350 (63.71%), Query Frame = 0


Query: 4   FLCFNLLFLFTLSI-----SFLLQRASASYAG-------SASSI-VNPAKVKQISWSPRY 63
           FL F+L FLFTL +     +  L R+S +  G       SASS   +P +V Q+SW+PR 
Sbjct: 6   FLAFSLCFLFTLPLISSAPNRFLTRSSNTRDGSVIKMKTSASSFGFDPTRVTQLSWTPRV 65

Query: 64  FNFSLSLFFFYYYYYYFLSFDDRLDLFTEPWSRFNLNDSSGLLCMKAKAELKRSSVADNL 123
           F            Y  FLS D+  D F +                 AK +L++S VADN 
Sbjct: 66  F-----------LYEGFLS-DEECDHFIK----------------LAKGKLEKSMVADND 125

Query: 124 SGKS-----KVSEVRTSSGAFIHKAK---DPIVSGIEDKIAAWTFLPKENGEDIQVLRYE 183
           SG+S      VS VR SS    +      D IVS +E K+AAWTFLP+ENGE +Q+L YE
Sbjct: 126 SGESVESEDSVSVVRQSSSFIANMDSLEIDDIVSNVEAKLAAWTFLPEENGESMQILHYE 185

Query: 184 YGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSAEESQRRQASETNED 243
            GQKY+ HFDYF D+ N+  GGHR+ATVLMYLS+VEKGGETVFP      + +A++  +D
Sbjct: 186 NGQKYEPHFDYFHDQANLELGGHRIATVLMYLSNVEKGGETVFP----MWKGKATQLKDD 245

Query: 244 -LSDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEKWSATKWIHVDSFD 303
             ++CAK+G AVKPRKGDALLFF+LHPNA  D++SLHG CPV+EGEKWSAT+WIHV SF+
Sbjct: 246 SWTECAKQGYAVKPRKGDALLFFNLHPNATTDSNSLHGSCPVVEGEKWSATRWIHVKSFE 305

Query: 304 MVMRDHTNCGDENPSCERWAELGECTNNPEYMVGSPELPGYCRKSCKACS 332
                 + C DEN SCE+WA+ GEC  NP YMVGS +  GYCRKSCKACS
Sbjct: 306 RAFNKQSGCMDENVSCEKWAKAGECQKNPTYMVGSDKDHGYCRKSCKACS 323

[Close]

BLAST of Chy6G111710 vs. TAIR 10
Match: AT3G28490.1 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 285.4 bits (729), Expect = 5.8e-77
Identity = 164/321 (51.09%), Postives = 203/321 (63.24%), Query Frame = 0


Query: 11  FLFTLSISFLLQRASASYAGSASSIVNPAKVKQISWSPRYFNFSLSLFFFYYYYYYFLSF 70
           +    S+S LL     S   S S  V+P ++ Q+SW+PR F            Y  FLS 
Sbjct: 5   YFLAFSLSLLL---IFSQISSFSFSVDPTRITQLSWTPRAF-----------LYKGFLS- 64

Query: 71  DDRLDLFTEPWSRFNLNDSSGLLCMKAKAELKRSS-VADNLSGKSKVSEVRTSSGAFIHK 130
           D+  D                 L   AK +L++S  VAD  SG+S+ SEVRTSSG F+ K
Sbjct: 65  DEECD----------------HLIKLAKGKLEKSMVVADVDSGESEDSEVRTSSGMFLTK 124

Query: 131 AKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMAT 190
            +D IV+ +E K+AAWTFLP+ENGE +Q+L YE GQKYD HFDYF DK  +  GGHR+AT
Sbjct: 125 RQDDIVANVEAKLAAWTFLPEENGEALQILHYENGQKYDPHFDYFYDKKALELGGHRIAT 184

Query: 191 VLMYLSDVEKGGETVFPSAEESQRRQASETNEDLSDCAKKGIAVKPRKGDALLFFSLHPN 250
           VLMYLS+V KGGETVFP+    + +     ++  S CAK+G AVKPRKGDALLFF+LH N
Sbjct: 185 VLMYLSNVTKGGETVFPN---WKGKTPQLKDDSWSKCAKQGYAVKPRKGDALLFFNLHLN 244

Query: 251 AIPDTSSLHGGCPVIEGEKWSATKWIHVDSFDMVMRDHTNCGDENPSCERWAELGECTNN 310
              D +SLHG CPVIEGEKWSAT+WIHV SF    +    C D++ SC+ WA+ GEC  N
Sbjct: 245 GTTDPNSLHGSCPVIEGEKWSATRWIHVRSFG---KKKLVCVDDHESCQEWADAGECEKN 288

Query: 311 PEYMVGSPELPGYCRKSCKAC 331
           P YMVGS    G+CRKSCKAC
Sbjct: 305 PMYMVGSETSLGFCRKSCKAC 288

The following BLAST results are available for this feature:

BLAST of Chy6G111710 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LAN3	1.6e-111	67.08	Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=...	[more]
F4JAU3	2.0e-106	63.24	Prolyl 4-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=P4H2 PE=1 SV=1	[more]
Q8L970	1.4e-88	53.51	Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=...	[more]
F4J0A8	8.1e-76	51.09	Probable prolyl 4-hydroxylase 6 OS=Arabidopsis thaliana OX=3702 GN=P4H6 PE=2 SV=...	[more]
Q9LN20	1.8e-54	47.68	Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=...	[more]

BLAST of Chy6G111710 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L5Q6	6.5e-153	86.10	Procollagen-proline 4-dioxygenase OS=Cucumis sativus OX=3659 GN=Csa_3G150820 PE=...	[more]
A0A1S3AWU7	1.8e-150	84.59	Procollagen-proline 4-dioxygenase OS=Cucumis melo OX=3656 GN=LOC103483779 PE=3 S...	[more]
A0A5A7U593	1.3e-148	83.69	Procollagen-proline 4-dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A6J1C7M6	8.9e-142	79.70	Procollagen-proline 4-dioxygenase OS=Momordica charantia OX=3673 GN=LOC111009125...	[more]
A0A6J1I971	1.1e-139	78.79	Procollagen-proline 4-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111472232 PE...	[more]

BLAST of Chy6G111710 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004134175.2	4.30e-195	86.10	probable prolyl 4-hydroxylase 4 [Cucumis sativus] >KGN57053.1 hypothetical prote...	[more]
XP_008438765.1	1.81e-192	84.59	PREDICTED: probable prolyl 4-hydroxylase 4 [Cucumis melo]	[more]
KAA0049426.1	4.44e-190	83.69	putative prolyl 4-hydroxylase 4 [Cucumis melo var. makuwa] >TYK16104.1 putative ...	[more]
XP_038903083.1	7.84e-187	81.87	probable prolyl 4-hydroxylase 4 [Benincasa hispida]	[more]
XP_022137761.1	6.58e-181	79.39	probable prolyl 4-hydroxylase 4 [Momordica charantia]	[more]

BLAST of Chy6G111710 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G18900.1	1.1e-112	67.08	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G06300.1	1.4e-107	63.24	P4H isoform 2	[more]
AT3G28480.1	1.0e-89	53.51	Oxoglutarate/iron-dependent oxygenase	[more]
AT3G28480.2	4.1e-83	51.14	Oxoglutarate/iron-dependent oxygenase	[more]
AT3G28490.1	5.8e-77	51.09	Oxoglutarate/iron-dependent oxygenase	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006620	Prolyl 4-hydroxylase, alpha subunit	SMART	SM00702	p4hc	coord: 65..276 e-value: 9.6E-46 score: 168.0
IPR003582	ShKT domain	SMART	SM00254	ShkT_1	coord: 289..331 e-value: 3.4E-7 score: 39.9
IPR003582	ShKT domain	PFAM	PF01549	ShK	coord: 289..330 e-value: 8.4E-4 score: 19.8
IPR003582	ShKT domain	PROSITE	PS51670	SHKT	coord: 290..330 score: 8.658319
None	No IPR available	GENE3D	2.60.120.620	q2cbj1_9rhob like domain	coord: 86..277 e-value: 1.6E-64 score: 219.5
None	No IPR available	PANTHER	PTHR10869:SF175	PROLYL 4-HYDROXYLASE SUBUNIT ALPHA-LIKE PROTEIN	coord: 5..53
None	No IPR available	PANTHER	PTHR10869:SF175	PROLYL 4-HYDROXYLASE SUBUNIT ALPHA-LIKE PROTEIN	coord: 93..330
IPR044862	Prolyl 4-hydroxylase alpha subunit, Fe(2+) 2OG dioxygenase domain	PFAM	PF13640	2OG-FeII_Oxy_3	coord: 156..276 e-value: 3.7E-21 score: 75.8
IPR045054	Prolyl 4-hydroxylase	PANTHER	PTHR10869	PROLYL 4-HYDROXYLASE ALPHA SUBUNIT	coord: 93..330
IPR045054	Prolyl 4-hydroxylase	PANTHER	PTHR10869	PROLYL 4-HYDROXYLASE ALPHA SUBUNIT	coord: 5..53
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 152..277 score: 12.667426

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy6G111710.1	Chy6G111710.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0018401	peptidyl-proline hydroxylation to 4-hydroxy-L-proline
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0031418	L-ascorbic acid binding
molecular_function	GO:0004656	procollagen-proline 4-dioxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen