Chy6G111320 (gene) Cucumber (hystrix) v1

Overview


Name	Chy6G111320
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	NEDD8-activating enzyme E1 catalytic subunit
Location	chrH06: 4123908 .. 4127526 (-)
RNA-Seq Expression	Chy6G111320
Synteny	Chy6G111320

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGGCTCTGTCATCCAATCCAGTCGATCGAGGGACCTCGATAAGCTCCTTCTTAGGCCCGGTAATCTCATCGGCGCCAATTTCGAGCCGGGTTCTCAAGTGGGTACTTCTTCTTCTCTCTGACATTCTTTCAAACACCTCGTGCTCACTACATGGTTCTGCAATCGTCTTACGACTGTGTTGTTTTGTATTGCAGTTGAGGGATGACCTTCAACAGTATGTGAAGGTGTTAGTCATAGGAGCTGGGGGGTTGGGCTGCGAGCTCCTGAAAGACTTGGCTTTGTCGGGCTTTCGGAATCTGGAAGTCATCGACATGGATCGCATCGAAGTTACTAATCTCAATCGTCAGTTTCTCTTCAGGTTTCAATTGTTTTCTTTGCTCTATTTGTATCTTTGGAGTTTTATTCGTAGTTGGTTTTTTTAATCTTTTAACAACCGGGGCCCGGGGGGTGGGTGTAGAACTCTCACTTTTTGTTGGGTGTATACTGAGCTGTAATGACATTTCAAATTGATCTCTATTTTTAAGTTACATCCATTACTGACTGATGATATTTATATTTATGATTTGATATCGGTAAAATCTGCACTAGTTATAGGAATTCGATGGATCGAATGTCACATTTTGTTACATTTTACTTGACTGGAGCCTTTCTTCATAGTTTGCTTCCCGTTTTGTGGACTTCTCTTTTTGTATGTTGATTGATGCATTTTTCTTTTCTCAATGAAAGTCCAACTTTTACATCAAAAAGAAAGAAGAAAAAAGAAAAGATTGATGGGTCGAATGTTCTTTCCTTTCAACCATTTGTACTAAAATGCTGAACCAGCTGAATTAGAACGGTGCTTAAATAAATTTTCCTGTAAGAAAGTTCTTTTCAGGATATTTTATTATTACGATATGATTTGACCTTGGAATCCTTGAGGAAATCACATTTCTCATATGTAAATCTGTATATGGCTTTACAGTAGTCATTTGATTAAGTACGGCAGTCAGAGGAATAACACTAAGGTAGTATCGCAGGCTTGAAGATGTTGGTAAGCCAAAAGCTGAGGTGGCTGCAAAACGTGTTATGGAGAGAGTTAGTGGTGTCAATATTCTGCCACATTTTTGCCGGATTGAGGACAAAGAAATTGAATTTTACAACGATTTTCACATAATCGCACTTGGTCTTGATTCAATTGAAGCTCGAAGTTACATCAATTCTGTTGCTTGTAGCTTCCTTGGTATGCTATTCTCTGACTTGCTATGTTCTTACGTCTTTTTGCCATCTTTTTTTCATTAAATGTTAATGTCATGTTGTTGATGATGAATGAGAATTTCATGTCCTTTATTTTTTCTTTGTAAATTTAGAGTATGATTCTGATGATAATCCATTAGAAGAAACAATTAAACCAATGGTAGATGGTGGAACTGAAGGCTTCAAGGGGCATGCAAGGGTAATTTTACCCGGGGTCACACCTTGCTTCGAGTGTACCATCTGGCTTTTTCCACCGCAAGTAAAGTTTCCATTATGTACTCTAGCAGAAACTCCTAGAACTGCTGCTCATTGTATCGAGTATGCTCATCTAATTAAATGGGATGAGGTAATACATTATTACCGGGCTTCTCGTTGATGATTGTGAAAGTACCCCGTTTGGGCTTACCAAGACTTTTTCTTTTAGGTCCATAGTGGAAAATCTTTTGATCCTGATGATTCCGAACACATGAAATGGGTTTACAGTGAGGTATGATAATTCCTGTAGATCCGGTAATTTTTTTCAAATAGTGATTCTGAACTTCTAATTTATCTTCCCACATCTATAGGCTCTTAAGAGGGCTGAGCTTTTTGGCATTCCGGGAGTTACATATTCACTAACTCAGGTACATGTTAGTCTTGGAATTTTCACATCACTGAAGTGATGGTTATTCTTTTTATCTGAACTTGTGGTTGTTCTGTTAGTAGGTACATATTATCTGCTTGCATCTTTTGTTAACTACTCACCTGCTGTTGTTTACAAATGCCCCTACTGATATTCATTTTCTAATGGTTTTTTAAGGGTGTTGTTAAGAATATCATACCTGCTATTGCTTCCACCAATGCAATTATATCAGCCGCATGTGCACTGGAAACGTTGAAGATTGTATCAGGATGCAGTAAAACTCTATCCAACTACTTAACGTGCGTACTGTTTGCTTTAACTATGTGACAATTCCTTTTCATTTTAATGTTATACGGGTTTAATAATAATTTTGTAGAGCAGCTTAATATTGTTCTTCTTTATTTCTTACTAAAAAATATATGGGTACATAACATGTCAGGCTGCATTGAGAAGTTTGGATTTATTATTATAGGCTCTTTGGGGAGCACAATAACATAGTATTTAGAGGATTGGAGAGGAATCCTAGTGGTGGTTCCTTCTTAGATTTCATGTTTTCCTCTAGGCTTCAATTTCGAAGATCATTTGTAATTATTCTTAAAAAGACAATTTTGTATGGCTGAACCCCTTTCTTTCTTGTGTGGATTTGGTTTTTCTCGAACCTTTTATTTATTTTTTCAATGAAATTTGTTGTTTCCTTTGAAAATACATTTTTGGATATATCGTGTATCATTTTTTGAGTTCTTTGCTCATAATCCAGGAATGTGGTTTATGTTATGATTGCTATTTTTACATTTTCATTTGTTCTGGTTTTCATTATGGTCGAAAAGTTATATGGTTCTCTTCCATCATTCTTATTATTCTTGTCATTTGTATTCTTGAATTGATAAGTCAGTCAATGCCTTGTTAATATACTAAACACTTTACGGTTCTTTCCATTATTTATTTTTTACACCTTTTAGATTAGAAGTCTAGCCTTCTATAGCCACCGATCTCTTCTTTCTATCAAGTGGGAAGTTTTTATTACAAATATATTGTTTCTATGTTGGAAGTTGAAGCTAGGCTTGCGTGTGAATGACACAATTTTCATAAATCATGTCTGTATTTGTAATAGCCTTTAACATTGTGCCATTATTTACTTTTATTTGTGACCATATAATTTCCAGATGCATTGAATTTAATGCCAAATGATCTTTTTTTTCAGATATAATGGTGCTGAAGGTCTCCACACTAAAGTGACCGAGTTTGTGAAAGATAAGGACTGTCTTGTTTGTGGTCCAGGCGTTCTTATTGAGCTGGATTCTTCCATTACTTTACAGAAGGTTGATATTTAGCTTGTGCCATCCACATGTTATATGCAACGTCACAGACATATCCATCTTGTATTCAGTTGTTGCTGTGTTAATTTCAGATTTTGAGAAATCTTGTTAATCCTTCATGACTGTTCCTCCCATTTTGAATTTGCCTGAAACAGTTCATTGACCTGCTAGAAGATCATCCCAAACTCTTGTTATCAAAGGCAAGTGTCACGCATCGAGGGAAGAATCTATACATGCAGGCACCTCCTGTACTTGAAGAGATGACCAGATCAAATTTGGGTGTTCCACTTTTTGTCTTGATGGGTAAAGTTTTGAAGGACGTAGTCCATGTGACTGGTGTGGCTTCTAAGAATGATAAGAAAACTTCCTGTCTTCGAAAATTACGTGTGGCTTTTAAGGGGGTTGACGGTGTTACGGATATGGACACTGCAAGTGGGGCTTAA

mRNA sequence

ATGGCGGGCTCTGTCATCCAATCCAGTCGATCGAGGGACCTCGATAAGCTCCTTCTTAGGCCCGGTAATCTCATCGGCGCCAATTTCGAGCCGGGTTCTCAATTGAGGGATGACCTTCAACAGTATGTGAAGGTGTTAGTCATAGGAGCTGGGGGGTTGGGCTGCGAGCTCCTGAAAGACTTGGCTTTGTCGGGCTTTCGGAATCTGGAAGTCATCGACATGGATCGCATCGAAGTTACTAATCTCAATCGTCAGTTTCTCTTCAGGCTTGAAGATGTTGGTAAGCCAAAAGCTGAGGTGGCTGCAAAACGTGTTATGGAGAGAGTTAGTGGTGTCAATATTCTGCCACATTTTTGCCGGATTGAGGACAAAGAAATTGAATTTTACAACGATTTTCACATAATCGCACTTGGTCTTGATTCAATTGAAGCTCGAAGTTACATCAATTCTGTTGCTTGTAGCTTCCTTGAGTATGATTCTGATGATAATCCATTAGAAGAAACAATTAAACCAATGGTAGATGGTGGAACTGAAGGCTTCAAGGGGCATGCAAGGGTAATTTTACCCGGGGTCACACCTTGCTTCGAGTGTACCATCTGGCTTTTTCCACCGCAAGTAAAGTTTCCATTATGTACTCTAGCAGAAACTCCTAGAACTGCTGCTCATTGTATCGAGTATGCTCATCTAATTAAATGGGATGAGGTCCATAGTGGAAAATCTTTTGATCCTGATGATTCCGAACACATGAAATGGGTTTACAGTGAGGCTCTTAAGAGGGCTGAGCTTTTTGGCATTCCGGGAGTTACATATTCACTAACTCAGGGTGTTGTTAAGAATATCATACCTGCTATTGCTTCCACCAATGCAATTATATCAGCCGCATGTGCACTGGAAACGTTGAAGATTGTATCAGGATGCAGTAAAACTCTATCCAACTACTTAACATATAATGGTGCTGAAGGTCTCCACACTAAAGTGACCGAGTTTGTGAAAGATAAGGACTGTCTTGTTTGTGGTCCAGGCGTTCTTATTGAGCTGGATTCTTCCATTACTTTACAGAAGTTCATTGACCTGCTAGAAGATCATCCCAAACTCTTGTTATCAAAGGCAAGTGTCACGCATCGAGGGAAGAATCTATACATGCAGGCACCTCCTGTACTTGAAGAGATGACCAGATCAAATTTGGGTGTTCCACTTTTTGTCTTGATGGGTAAAGTTTTGAAGGACGTAGTCCATGTGACTGGTGTGGCTTCTAAGAATGATAAGAAAACTTCCTGTCTTCGAAAATTACGTGTGGCTTTTAAGGGGGTTGACGGTGTTACGGATATGGACACTGCAAGTGGGGCTTAA

Coding sequence (CDS)

ATGGCGGGCTCTGTCATCCAATCCAGTCGATCGAGGGACCTCGATAAGCTCCTTCTTAGGCCCGGTAATCTCATCGGCGCCAATTTCGAGCCGGGTTCTCAATTGAGGGATGACCTTCAACAGTATGTGAAGGTGTTAGTCATAGGAGCTGGGGGGTTGGGCTGCGAGCTCCTGAAAGACTTGGCTTTGTCGGGCTTTCGGAATCTGGAAGTCATCGACATGGATCGCATCGAAGTTACTAATCTCAATCGTCAGTTTCTCTTCAGGCTTGAAGATGTTGGTAAGCCAAAAGCTGAGGTGGCTGCAAAACGTGTTATGGAGAGAGTTAGTGGTGTCAATATTCTGCCACATTTTTGCCGGATTGAGGACAAAGAAATTGAATTTTACAACGATTTTCACATAATCGCACTTGGTCTTGATTCAATTGAAGCTCGAAGTTACATCAATTCTGTTGCTTGTAGCTTCCTTGAGTATGATTCTGATGATAATCCATTAGAAGAAACAATTAAACCAATGGTAGATGGTGGAACTGAAGGCTTCAAGGGGCATGCAAGGGTAATTTTACCCGGGGTCACACCTTGCTTCGAGTGTACCATCTGGCTTTTTCCACCGCAAGTAAAGTTTCCATTATGTACTCTAGCAGAAACTCCTAGAACTGCTGCTCATTGTATCGAGTATGCTCATCTAATTAAATGGGATGAGGTCCATAGTGGAAAATCTTTTGATCCTGATGATTCCGAACACATGAAATGGGTTTACAGTGAGGCTCTTAAGAGGGCTGAGCTTTTTGGCATTCCGGGAGTTACATATTCACTAACTCAGGGTGTTGTTAAGAATATCATACCTGCTATTGCTTCCACCAATGCAATTATATCAGCCGCATGTGCACTGGAAACGTTGAAGATTGTATCAGGATGCAGTAAAACTCTATCCAACTACTTAACATATAATGGTGCTGAAGGTCTCCACACTAAAGTGACCGAGTTTGTGAAAGATAAGGACTGTCTTGTTTGTGGTCCAGGCGTTCTTATTGAGCTGGATTCTTCCATTACTTTACAGAAGTTCATTGACCTGCTAGAAGATCATCCCAAACTCTTGTTATCAAAGGCAAGTGTCACGCATCGAGGGAAGAATCTATACATGCAGGCACCTCCTGTACTTGAAGAGATGACCAGATCAAATTTGGGTGTTCCACTTTTTGTCTTGATGGGTAAAGTTTTGAAGGACGTAGTCCATGTGACTGGTGTGGCTTCTAAGAATGATAAGAAAACTTCCTGTCTTCGAAAATTACGTGTGGCTTTTAAGGGGGTTGACGGTGTTACGGATATGGACACTGCAAGTGGGGCTTAA

Protein sequence

MAGSVIQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKSFDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFVLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVAFKGVDGVTDMDTASGA*

Homology

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BLAST of Chy6G111320 vs. ExPASy Swiss-Prot
Match: O65041 (NEDD8-activating enzyme E1 catalytic subunit OS=Arabidopsis thaliana OX=3702 GN=ECR1 PE=1 SV=2)

HSP 1 Score: 726.5 bits (1874), Expect = 1.9e-208
Identity = 354/445 (79.55%), Postives = 389/445 (87.42%), Query Frame = 0


Query: 6   IQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSG 65
           +  S++RDLDKLLLR GNL+   F PG  LRDD++ YV++LVIGAGGLGCELLKDLALSG
Sbjct: 10  VPQSKTRDLDKLLLRHGNLVDPGFVPGPGLRDDIRDYVRILVIGAGGLGCELLKDLALSG 69

Query: 66  FRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKE 125
           FRNLEVIDMDRIEVTNLNRQFLFR+EDVGKPKAEVAAKRVMERVSGV I+PHF RIEDKE
Sbjct: 70  FRNLEVIDMDRIEVTNLNRQFLFRIEDVGKPKAEVAAKRVMERVSGVEIVPHFSRIEDKE 129

Query: 126 IEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHAR 185
           IEFYNDF+IIALGLDSIEAR YIN VAC FLEY+ DD P  ETIKPMVDGGTEGFKGHAR
Sbjct: 130 IEFYNDFNIIALGLDSIEARKYINGVACGFLEYNEDDTPKRETIKPMVDGGTEGFKGHAR 189

Query: 186 VILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKSFDPDD 245
           VILPGVTPCFECTI+LFPPQVKFPLCTLAETPR AAHCIEYAHLI+W+ VH GK+FDPD+
Sbjct: 190 VILPGVTPCFECTIYLFPPQVKFPLCTLAETPRNAAHCIEYAHLIQWETVHRGKTFDPDE 249

Query: 246 SEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSG 305
            EHMKWVY EA++RAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVS 
Sbjct: 250 PEHMKWVYDEAIRRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSA 309

Query: 306 CSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDHPKL 365
           CSKTL NYLTYNG EGL+T+VT+F +D +CLVCGPG+LIELD+S+TL KFI++LEDHPKL
Sbjct: 310 CSKTLVNYLTYNGGEGLYTEVTKFERDTECLVCGPGILIELDTSVTLSKFIEMLEDHPKL 369

Query: 366 LLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFVLMGKVLKDVVHVTGV-ASKNDKKT 425
           LLSKASV      LYMQAPPVLEE  R  L  PL+ LMG+V KD +HV G  A KN++K 
Sbjct: 370 LLSKASVKQGENTLYMQAPPVLEEFHRPKLSKPLYDLMGRVQKDTIHVFGQRALKNNEKE 429

Query: 426 SCLRKLRVAFKGVDGVTDMDTASGA 450
           SC  K+RV FKG DGV DMDTA GA
Sbjct: 430 SCTTKVRVVFKGADGVADMDTAIGA 454

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BLAST of Chy6G111320 vs. ExPASy Swiss-Prot
Match: Q9V6U8 (Nedd8-activating enzyme E1 catalytic subunit OS=Drosophila melanogaster OX=7227 GN=Uba3 PE=1 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 2.3e-105
Identity = 199/402 (49.50%), Postives = 259/402 (64.43%), Query Frame = 0


Query: 3   GSVIQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLA 62
           G ++QS R   L  +L R G      F   S+  + LQ   +VL+IGAGGLGCELLKDLA
Sbjct: 9   GLILQSKRFNGLRNILEREGPFCKDGFAASSENLEFLQTKCQVLIIGAGGLGCELLKDLA 68

Query: 63  LSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIE 122
           L GF NL VIDMD IE++NLNRQFLFR  D+G  KAE AA+ +  RV    + PHF +I+
Sbjct: 69  LMGFGNLHVIDMDTIELSNLNRQFLFRRTDIGASKAECAARFINARVPTCRVTPHFKKIQ 128

Query: 123 DKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKG 182
           D +  FY  FH++  GLDSI AR +IN +  S L Y+ D      +I PM+DGGTEGFKG
Sbjct: 129 DFDESFYQQFHLVVCGLDSIVARRWINGMLLSMLRYEEDGTIDTSSIVPMIDGGTEGFKG 188

Query: 183 HARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHS-GKSF 242
           +ARVILPG T C ECT+ LFPPQV +PLCT+A TPR   HCIEY  +I+W++ +  G   
Sbjct: 189 NARVILPGFTACIECTLDLFPPQVNYPLCTIANTPRLPEHCIEYVKIIQWEKQNPFGVPL 248

Query: 243 DPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLK 302
           D DD +H+ W+Y  AL+R+  F I GVTY L QGVVK+IIPA+ASTNA I+AACALE  K
Sbjct: 249 DGDDPQHIGWIYERALERSNEFNITGVTYRLVQGVVKHIIPAVASTNAAIAAACALEVFK 308

Query: 303 IVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCG--PGVL-IELDSSITLQKFIDL 362
           + + C  +++NYL +N  +G++T   E  K ++CL C   P  L IE  ++ TL+  I L
Sbjct: 309 LATSCYDSMANYLNFNDLDGIYTYTYEAEKSENCLACSNTPQPLPIEDPNTTTLEDVIKL 368

Query: 363 LEDHPKLLLSKASVT-----HRGKNLYMQAPPVLEEMTRSNL 396
           L D P+  L   ++T      + + LYM     +EE TR NL
Sbjct: 369 LCDSPRFQLKSPALTTVMKDGKRRTLYMSGVKSIEEATRKNL 410

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BLAST of Chy6G111320 vs. ExPASy Swiss-Prot
Match: Q54QG9 (NEDD8-activating enzyme E1 catalytic subunit OS=Dictyostelium discoideum OX=44689 GN=uba3 PE=1 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 8.7e-105
Identity = 199/405 (49.14%), Postives = 264/405 (65.19%), Query Frame = 0


Query: 1   MAGSVIQSSRSRDLDKLLLRPGNLIGANFEPGSQ----LRDDLQQYVKVLVIGAGGLGCE 60
           M  SV    R  D++K++ R G     +FEP ++    + + LQ   KVLVIGAGGLGCE
Sbjct: 4   MDTSVDLPGRWIDIEKIIKRTGPFASPSFEPDTKASPNIMNGLQNDFKVLVIGAGGLGCE 63

Query: 61  LLKDLALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILP 120
           +LK+LALSGFRN++VIDMD I+++NLNRQFLFR +DVGK KAEVAA  +  R++G N+ P
Sbjct: 64  ILKNLALSGFRNIDVIDMDTIDISNLNRQFLFRRKDVGKSKAEVAAAFINSRITGCNVTP 123

Query: 121 HFCRIEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGG 180
           H CRI+DK+ ++Y  F I+  GLDSIEAR +IN +  + +  +   +   +TI P+VDGG
Sbjct: 124 HKCRIQDKDEDYYRQFKIVIAGLDSIEARRWINGLLVNLVVVNDSGDIEPDTIIPLVDGG 183

Query: 181 TEGFKGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVH 240
           TEGFKG ARVILP ++ CFEC++  FPPQV + +CT+A TPR   HCI++A L    +  
Sbjct: 184 TEGFKGQARVILPKISSCFECSLDAFPPQVSYAICTIANTPRVPEHCIQWALLFGLQDAT 243

Query: 241 SGKSFDP-----DDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAII 300
             K FDP     D+ +HM W++  A KRAE F I GVTY LTQGV KNIIPAIASTNAII
Sbjct: 244 LEKPFDPKQFDNDNPDHMNWLFECAKKRAEKFNINGVTYKLTQGVAKNIIPAIASTNAII 303

Query: 301 SAACALETLKIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPG-VLIELDSSI 360
           +AAC  E  K  +  S  L+NY+ YNG  G++T   E+   + C VCG   V  E+D S 
Sbjct: 304 AAACCNEVFKFCTDSSGYLNNYMMYNGLNGVYTFTFEYEIKEGCAVCGTNLVTFEIDKSN 363

Query: 361 TLQKFIDLLEDHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNL 396
           TL  F++ +    +    K S+   G+NLYMQ   +L + T  NL
Sbjct: 364 TLSTFLEKITTDSRFQFKKPSLRSNGRNLYMQG--LLHQSTVPNL 406

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BLAST of Chy6G111320 vs. ExPASy Swiss-Prot
Match: Q8TBC4 (NEDD8-activating enzyme E1 catalytic subunit OS=Homo sapiens OX=9606 GN=UBA3 PE=1 SV=2)

HSP 1 Score: 373.6 bits (958), Expect = 3.1e-102
Identity = 196/399 (49.12%), Postives = 260/399 (65.16%), Query Frame = 0


Query: 10  RSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNL 69
           R   + K L R G     +FEP ++    L    KVLVIGAGGLGCELLK+LALSGFR +
Sbjct: 37  RWNHVKKFLERSGPFTHPDFEPSTESLQFLLDTCKVLVIGAGGLGCELLKNLALSGFRQI 96

Query: 70  EVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFY 129
            VIDMD I+V+NLNRQFLFR +D+G+PKAEVAA+ + +RV   N++PHF +I+D    FY
Sbjct: 97  HVIDMDTIDVSNLNRQFLFRPKDIGRPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFY 156

Query: 130 NDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLE-ETIKPMVDGGTEGFKGHARVIL 189
             FHII  GLDSI AR +IN +  S L Y  +D  L+  +I P++DGGTEGFKG+ARVIL
Sbjct: 157 RQFHIIVCGLDSIIARRWINGMLISLLNY--EDGVLDPSSIVPLIDGGTEGFKGNARVIL 216

Query: 190 PGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVH---SGKSFDPDD 249
           PG+T C ECT+ L+PPQV FP+CT+A  PR   HCIEY  +++W +      G   D DD
Sbjct: 217 PGMTACIECTLELYPPQVNFPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGEGVPLDGDD 276

Query: 250 SEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSG 309
            EH++W++ ++L+RA  + I GVTY LTQGVVK IIPA+ASTNA+I+A CA E  KI + 
Sbjct: 277 PEHIQWIFQKSLERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVIAAVCATEVFKIATS 336

Query: 310 CSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGP-GVLIELDSSITLQKFIDLLEDHPK 369
               L+NYL +N  +GL+T   E  + ++C  C      I+   S  LQ+ +D L +   
Sbjct: 337 AYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDYLTNSAS 396

Query: 370 LLLSKASVTH--RGKN--LYMQAPPVLEEMTRSNLGVPL 400
           L +   ++T    GKN  LY+Q+   +EE TR NL   L
Sbjct: 397 LQMKSPAITATLEGKNRTLYLQSVTSIEERTRPNLSKTL 433

[Close]

BLAST of Chy6G111320 vs. ExPASy Swiss-Prot
Match: Q5R4A0 (NEDD8-activating enzyme E1 catalytic subunit OS=Pongo abelii OX=9601 GN=UBA3 PE=2 SV=2)

HSP 1 Score: 373.6 bits (958), Expect = 3.1e-102
Identity = 195/399 (48.87%), Postives = 260/399 (65.16%), Query Frame = 0


Query: 10  RSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNL 69
           R   + K L R G     +FEP ++    L    KVLVIGAGGLGCELLK+LALSGFR +
Sbjct: 37  RWNHVKKFLERSGPFTHPDFEPSTESLQFLLDTCKVLVIGAGGLGCELLKNLALSGFRQI 96

Query: 70  EVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFY 129
            VIDMD I+V+NLNRQFLFR +D+G+PKAEVAA+ + +RV   N++PHF +I+D    FY
Sbjct: 97  HVIDMDTIDVSNLNRQFLFRPKDIGRPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFY 156

Query: 130 NDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLE-ETIKPMVDGGTEGFKGHARVIL 189
             FHII  GLDSI AR +IN +  S L Y  +D  L+  +I P++DGGTEGFKG+ARVIL
Sbjct: 157 RQFHIIVCGLDSIIARRWINGMLISLLNY--EDGVLDPSSIVPLIDGGTEGFKGNARVIL 216

Query: 190 PGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVH---SGKSFDPDD 249
           PG+T C ECT+ L+PPQV FP+CT+A  PR   HCIEY  +++W +      G   D DD
Sbjct: 217 PGMTACIECTLELYPPQVNFPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGEGVPLDGDD 276

Query: 250 SEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSG 309
            EH++W++ ++L+RA  + I GVTY LTQGVVK IIPA+ASTNA+++A CA E  KI + 
Sbjct: 277 PEHIQWIFQKSLERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVVAAVCATEVFKIATS 336

Query: 310 CSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGP-GVLIELDSSITLQKFIDLLEDHPK 369
               L+NYL +N  +GL+T   E  + ++C  C      I+   S  LQ+ +D L +   
Sbjct: 337 AYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDFLTNSAS 396

Query: 370 LLLSKASVTH--RGKN--LYMQAPPVLEEMTRSNLGVPL 400
           L +   ++T    GKN  LY+Q+   +EE TR NL   L
Sbjct: 397 LQMKSPAITATLEGKNRTLYLQSVTSIEERTRPNLSKTL 433

[Close]

BLAST of Chy6G111320 vs. ExPASy TrEMBL
Match: A0A0A0L8U2 (NEDD8-activating enzyme E1 catalytic subunit OS=Cucumis sativus OX=3659 GN=Csa_3G149920 PE=3 SV=1)

HSP 1 Score: 902.5 bits (2331), Expect = 7.0e-259
Identity = 447/449 (99.55%), Postives = 448/449 (99.78%), Query Frame = 0


Query: 1   MAGSVIQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MAGSV+QSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD
Sbjct: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKS 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKS
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKS 240

Query: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
           FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL
Sbjct: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300

Query: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360
           KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFVLMGKVLKDVVHVTGVASKN 420
           DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLF LMGKVLKDVVHVTGVASKN
Sbjct: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420

Query: 421 DKKTSCLRKLRVAFKGVDGVTDMDTASGA 450
           DKKTSCLRKLRVAFKGVDGVTDMDTASGA
Sbjct: 421 DKKTSCLRKLRVAFKGVDGVTDMDTASGA 449

[Close]

BLAST of Chy6G111320 vs. ExPASy TrEMBL
Match: A0A1S3AXQ8 (NEDD8-activating enzyme E1 catalytic subunit OS=Cucumis melo OX=3656 GN=LOC103483734 PE=3 SV=1)

HSP 1 Score: 899.0 bits (2322), Expect = 7.8e-258
Identity = 445/449 (99.11%), Postives = 448/449 (99.78%), Query Frame = 0


Query: 1   MAGSVIQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MAGSV+QSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD
Sbjct: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKS 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGK+
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240

Query: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
           FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL
Sbjct: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300

Query: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360
           KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFVLMGKVLKDVVHVTGVASKN 420
           D+PKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLF LMGKVLKDVVHVTGVASKN
Sbjct: 361 DYPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420

Query: 421 DKKTSCLRKLRVAFKGVDGVTDMDTASGA 450
           DKKTSCLRKLRVAFKGVDGVTDMDTASGA
Sbjct: 421 DKKTSCLRKLRVAFKGVDGVTDMDTASGA 449

[Close]

BLAST of Chy6G111320 vs. ExPASy TrEMBL
Match: A0A5D3D1R8 (NEDD8-activating enzyme E1 catalytic subunit OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00180 PE=3 SV=1)

HSP 1 Score: 897.5 bits (2318), Expect = 2.3e-257
Identity = 444/449 (98.89%), Postives = 447/449 (99.55%), Query Frame = 0


Query: 1   MAGSVIQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MAGSV+QSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD
Sbjct: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKS 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGK+
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240

Query: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
           FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL
Sbjct: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300

Query: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360
           KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFVLMGKVLKDVVHVTGVASKN 420
           D+PKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLF LMGKVLKDVVHVTGVASKN
Sbjct: 361 DYPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420

Query: 421 DKKTSCLRKLRVAFKGVDGVTDMDTASGA 450
           DKKTSCLRKLRV FKGVDGVTDMDTASGA
Sbjct: 421 DKKTSCLRKLRVTFKGVDGVTDMDTASGA 449

[Close]

BLAST of Chy6G111320 vs. ExPASy TrEMBL
Match: A0A6J1CA17 (NEDD8-activating enzyme E1 catalytic subunit OS=Momordica charantia OX=3673 GN=LOC111009338 PE=3 SV=1)

HSP 1 Score: 883.6 bits (2282), Expect = 3.4e-253
Identity = 434/449 (96.66%), Postives = 444/449 (98.89%), Query Frame = 0


Query: 1   MAGSVIQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MAGSV+QSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLV+GAGGLGCELLKD
Sbjct: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVVGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRL+DVGKPKAEVAAKRVMERVSGVNI+PHFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLQDVGKPKAEVAAKRVMERVSGVNIVPHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYIN+VACSFLEYDSDD+PLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINAVACSFLEYDSDDSPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKS 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGK+
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKT 240

Query: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
           FDPDD  HMKWVY+EA+KRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL
Sbjct: 241 FDPDDPVHMKWVYNEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300

Query: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360
           KIVSGCSKTLSNYLTYNGAEGLHTKVTEFV+DKDCLVCGPGVLIELDSSITLQKFIDLLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVRDKDCLVCGPGVLIELDSSITLQKFIDLLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFVLMGKVLKDVVHVTGVASKN 420
           DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNL VPLF LMGKVLKDVVHVTGVASKN
Sbjct: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKVLKDVVHVTGVASKN 420

Query: 421 DKKTSCLRKLRVAFKGVDGVTDMDTASGA 450
           DKKTSCLRKLRVAFKGVDGVTDMDTA GA
Sbjct: 421 DKKTSCLRKLRVAFKGVDGVTDMDTAGGA 449

[Close]

BLAST of Chy6G111320 vs. ExPASy TrEMBL
Match: A0A6J1C926 (NEDD8-activating enzyme E1 catalytic subunit OS=Momordica charantia OX=3673 GN=LOC111009338 PE=3 SV=1)

HSP 1 Score: 879.8 bits (2272), Expect = 4.9e-252
Identity = 432/446 (96.86%), Postives = 442/446 (99.10%), Query Frame = 0


Query: 1   MAGSVIQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MAGSV+QSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLV+GAGGLGCELLKD
Sbjct: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVVGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRL+DVGKPKAEVAAKRVMERVSGVNI+PHFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLQDVGKPKAEVAAKRVMERVSGVNIVPHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYIN+VACSFLEYDSDD+PLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINAVACSFLEYDSDDSPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKS 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGK+
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKT 240

Query: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
           FDPDD  HMKWVY+EA+KRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL
Sbjct: 241 FDPDDPVHMKWVYNEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300

Query: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360
           KIVSGCSKTLSNYLTYNGAEGLHTKVTEFV+DKDCLVCGPGVLIELDSSITLQKFIDLLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVRDKDCLVCGPGVLIELDSSITLQKFIDLLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFVLMGKVLKDVVHVTGVASKN 420
           DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNL VPLF LMGKVLKDVVHVTGVASKN
Sbjct: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKVLKDVVHVTGVASKN 420

Query: 421 DKKTSCLRKLRVAFKGVDGVTDMDTA 447
           DKKTSCLRKLRVAFKGVDGVTDMDTA
Sbjct: 421 DKKTSCLRKLRVAFKGVDGVTDMDTA 446

[Close]

BLAST of Chy6G111320 vs. NCBI nr
Match: XP_004134149.1 (NEDD8-activating enzyme E1 catalytic subunit [Cucumis sativus] >KGN57012.1 hypothetical protein Csa_009619 [Cucumis sativus])

HSP 1 Score: 899 bits (2323), Expect = 0.0
Identity = 447/449 (99.55%), Postives = 448/449 (99.78%), Query Frame = 0


Query: 1   MAGSVIQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MAGSV+QSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD
Sbjct: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKS 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKS
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKS 240

Query: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
           FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL
Sbjct: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300

Query: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360
           KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFVLMGKVLKDVVHVTGVASKN 420
           DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLF LMGKVLKDVVHVTGVASKN
Sbjct: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420

Query: 421 DKKTSCLRKLRVAFKGVDGVTDMDTASGA 449
           DKKTSCLRKLRVAFKGVDGVTDMDTASGA
Sbjct: 421 DKKTSCLRKLRVAFKGVDGVTDMDTASGA 449

[Close]

BLAST of Chy6G111320 vs. NCBI nr
Match: XP_008438709.1 (PREDICTED: NEDD8-activating enzyme E1 catalytic subunit [Cucumis melo])

HSP 1 Score: 895 bits (2314), Expect = 0.0
Identity = 445/449 (99.11%), Postives = 448/449 (99.78%), Query Frame = 0


Query: 1   MAGSVIQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MAGSV+QSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD
Sbjct: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKS 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGK+
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240

Query: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
           FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL
Sbjct: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300

Query: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360
           KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFVLMGKVLKDVVHVTGVASKN 420
           D+PKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLF LMGKVLKDVVHVTGVASKN
Sbjct: 361 DYPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420

Query: 421 DKKTSCLRKLRVAFKGVDGVTDMDTASGA 449
           DKKTSCLRKLRVAFKGVDGVTDMDTASGA
Sbjct: 421 DKKTSCLRKLRVAFKGVDGVTDMDTASGA 449

[Close]

BLAST of Chy6G111320 vs. NCBI nr
Match: KAA0049385.1 (NEDD8-activating enzyme E1 catalytic subunit [Cucumis melo var. makuwa] >TYK17174.1 NEDD8-activating enzyme E1 catalytic subunit [Cucumis melo var. makuwa])

HSP 1 Score: 894 bits (2310), Expect = 0.0
Identity = 444/449 (98.89%), Postives = 447/449 (99.55%), Query Frame = 0


Query: 1   MAGSVIQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MAGSV+QSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD
Sbjct: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKS 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGK+
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240

Query: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
           FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL
Sbjct: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300

Query: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360
           KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFVLMGKVLKDVVHVTGVASKN 420
           D+PKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLF LMGKVLKDVVHVTGVASKN
Sbjct: 361 DYPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420

Query: 421 DKKTSCLRKLRVAFKGVDGVTDMDTASGA 449
           DKKTSCLRKLRV FKGVDGVTDMDTASGA
Sbjct: 421 DKKTSCLRKLRVTFKGVDGVTDMDTASGA 449

[Close]

BLAST of Chy6G111320 vs. NCBI nr
Match: XP_038897781.1 (NEDD8-activating enzyme E1 catalytic subunit [Benincasa hispida])

HSP 1 Score: 894 bits (2309), Expect = 0.0
Identity = 442/449 (98.44%), Postives = 447/449 (99.55%), Query Frame = 0


Query: 1   MAGSVIQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MAGS++QSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD
Sbjct: 1   MAGSIVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNI+PHFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNIVPHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKS 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGK+
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240

Query: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
           FDPDDSEHMKWVYSEA+KRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL
Sbjct: 241 FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300

Query: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360
           KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFVLMGKVLKDVVHVTGVASKN 420
           DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLF LMGKVLKD VHVTGVASKN
Sbjct: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDAVHVTGVASKN 420

Query: 421 DKKTSCLRKLRVAFKGVDGVTDMDTASGA 449
           DKKTSCLRKLRVAFKGVDGVTDMDTASGA
Sbjct: 421 DKKTSCLRKLRVAFKGVDGVTDMDTASGA 449

[Close]

BLAST of Chy6G111320 vs. NCBI nr
Match: XP_022138082.1 (NEDD8-activating enzyme E1 catalytic subunit isoform X4 [Momordica charantia])

HSP 1 Score: 880 bits (2274), Expect = 0.0
Identity = 434/449 (96.66%), Postives = 444/449 (98.89%), Query Frame = 0


Query: 1   MAGSVIQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MAGSV+QSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLV+GAGGLGCELLKD
Sbjct: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVVGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRL+DVGKPKAEVAAKRVMERVSGVNI+PHFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLQDVGKPKAEVAAKRVMERVSGVNIVPHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYIN+VACSFLEYDSDD+PLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINAVACSFLEYDSDDSPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKS 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGK+
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKT 240

Query: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
           FDPDD  HMKWVY+EA+KRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL
Sbjct: 241 FDPDDPVHMKWVYNEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300

Query: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360
           KIVSGCSKTLSNYLTYNGAEGLHTKVTEFV+DKDCLVCGPGVLIELDSSITLQKFIDLLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVRDKDCLVCGPGVLIELDSSITLQKFIDLLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFVLMGKVLKDVVHVTGVASKN 420
           DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNL VPLF LMGKVLKDVVHVTGVASKN
Sbjct: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKVLKDVVHVTGVASKN 420

Query: 421 DKKTSCLRKLRVAFKGVDGVTDMDTASGA 449
           DKKTSCLRKLRVAFKGVDGVTDMDTA GA
Sbjct: 421 DKKTSCLRKLRVAFKGVDGVTDMDTAGGA 449

[Close]

BLAST of Chy6G111320 vs. TAIR 10
Match: AT5G19180.1 (E1 C-terminal related 1 )

HSP 1 Score: 726.5 bits (1874), Expect = 1.3e-209
Identity = 354/445 (79.55%), Postives = 389/445 (87.42%), Query Frame = 0


Query: 6   IQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSG 65
           +  S++RDLDKLLLR GNL+   F PG  LRDD++ YV++LVIGAGGLGCELLKDLALSG
Sbjct: 10  VPQSKTRDLDKLLLRHGNLVDPGFVPGPGLRDDIRDYVRILVIGAGGLGCELLKDLALSG 69

Query: 66  FRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKE 125
           FRNLEVIDMDRIEVTNLNRQFLFR+EDVGKPKAEVAAKRVMERVSGV I+PHF RIEDKE
Sbjct: 70  FRNLEVIDMDRIEVTNLNRQFLFRIEDVGKPKAEVAAKRVMERVSGVEIVPHFSRIEDKE 129

Query: 126 IEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHAR 185
           IEFYNDF+IIALGLDSIEAR YIN VAC FLEY+ DD P  ETIKPMVDGGTEGFKGHAR
Sbjct: 130 IEFYNDFNIIALGLDSIEARKYINGVACGFLEYNEDDTPKRETIKPMVDGGTEGFKGHAR 189

Query: 186 VILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKSFDPDD 245
           VILPGVTPCFECTI+LFPPQVKFPLCTLAETPR AAHCIEYAHLI+W+ VH GK+FDPD+
Sbjct: 190 VILPGVTPCFECTIYLFPPQVKFPLCTLAETPRNAAHCIEYAHLIQWETVHRGKTFDPDE 249

Query: 246 SEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSG 305
            EHMKWVY EA++RAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVS 
Sbjct: 250 PEHMKWVYDEAIRRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSA 309

Query: 306 CSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDHPKL 365
           CSKTL NYLTYNG EGL+T+VT+F +D +CLVCGPG+LIELD+S+TL KFI++LEDHPKL
Sbjct: 310 CSKTLVNYLTYNGGEGLYTEVTKFERDTECLVCGPGILIELDTSVTLSKFIEMLEDHPKL 369

Query: 366 LLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFVLMGKVLKDVVHVTGV-ASKNDKKT 425
           LLSKASV      LYMQAPPVLEE  R  L  PL+ LMG+V KD +HV G  A KN++K 
Sbjct: 370 LLSKASVKQGENTLYMQAPPVLEEFHRPKLSKPLYDLMGRVQKDTIHVFGQRALKNNEKE 429

Query: 426 SCLRKLRVAFKGVDGVTDMDTASGA 450
           SC  K+RV FKG DGV DMDTA GA
Sbjct: 430 SCTTKVRVVFKGADGVADMDTAIGA 454

[Close]

BLAST of Chy6G111320 vs. TAIR 10
Match: AT2G21470.1 (SUMO-activating enzyme 2 )

HSP 1 Score: 152.9 bits (385), Expect = 6.1e-37
Identity = 79/203 (38.92%), Postives = 119/203 (58.62%), Query Frame = 0


Query: 44  KVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAK 103
           KVL++GAGG+GCELLK LALSGF ++ +IDMD IEV+NLNRQFLFR   VG+ KA+VA  
Sbjct: 14  KVLMVGAGGIGCELLKTLALSGFEDIHIIDMDTIEVSNLNRQFLFRRSHVGQSKAKVARD 73

Query: 104 RVMERVSGVNILPHFCRIEDKE--IEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSD 163
            V+     +NI  +   +++ E  ++F+  F ++  GLD+++AR ++N +  +       
Sbjct: 74  AVLRFRPNINIRSYHANVKNPEFDVDFFKQFDVVLNGLDNLDARRHVNRLCLA------- 133

Query: 164 DNPLEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAA 223
                    P+V+ GT GF G   V + G T C+EC     P    +P+CT+  TP    
Sbjct: 134 ------ADVPLVESGTTGFLGQVTVHIKGKTECYECQ--TKPAPKTYPVCTITSTPTKFV 193

Query: 224 HCIEYAHLIKWDEVHSGKSFDPD 245
           HCI +A  + + ++   K+ D D
Sbjct: 194 HCIVWAKDLLFAKLFGDKNQDND 201

HSP 2 Score: 52.4 bits (124), Expect = 1.1e-06
Identity = 42/145 (28.97%), Postives = 71/145 (48.97%), Query Frame = 0


Query: 240 SFDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALET 299
           +FD DD   +++V + A  RAE FGIP  +    +G+  NI+ A+A+TNAII+    +E 
Sbjct: 335 TFDKDDQLAVEFVTAAANIRAESFGIPLHSLFEAKGIAGNIVHAVATTNAIIAGLIVIEA 394

Query: 300 LKIV-SGCSKTLSNY-LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFID 359
           +K++     K    Y L +   + L   +  +  +  C VC    L+ L+ +    K  D
Sbjct: 395 IKVLKKDVDKFRMTYCLEHPSKKLLLMPIEPYEPNPACYVCSETPLV-LEINTRKSKLRD 454

Query: 360 LLED--HPKLLLSKASVTHRGKNLY 381
           L++     KL ++   + H    LY
Sbjct: 455 LVDKIVKTKLGMNLPLIMHGNSLLY 478

[Close]

BLAST of Chy6G111320 vs. TAIR 10
Match: AT2G21470.2 (SUMO-activating enzyme 2 )

HSP 1 Score: 152.9 bits (385), Expect = 6.1e-37
Identity = 79/203 (38.92%), Postives = 119/203 (58.62%), Query Frame = 0


Query: 44  KVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAK 103
           KVL++GAGG+GCELLK LALSGF ++ +IDMD IEV+NLNRQFLFR   VG+ KA+VA  
Sbjct: 14  KVLMVGAGGIGCELLKTLALSGFEDIHIIDMDTIEVSNLNRQFLFRRSHVGQSKAKVARD 73

Query: 104 RVMERVSGVNILPHFCRIEDKE--IEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSD 163
            V+     +NI  +   +++ E  ++F+  F ++  GLD+++AR ++N +  +       
Sbjct: 74  AVLRFRPNINIRSYHANVKNPEFDVDFFKQFDVVLNGLDNLDARRHVNRLCLA------- 133

Query: 164 DNPLEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAA 223
                    P+V+ GT GF G   V + G T C+EC     P    +P+CT+  TP    
Sbjct: 134 ------ADVPLVESGTTGFLGQVTVHIKGKTECYECQ--TKPAPKTYPVCTITSTPTKFV 193

Query: 224 HCIEYAHLIKWDEVHSGKSFDPD 245
           HCI +A  + + ++   K+ D D
Sbjct: 194 HCIVWAKDLLFAKLFGDKNQDND 201

HSP 2 Score: 52.4 bits (124), Expect = 1.1e-06
Identity = 42/145 (28.97%), Postives = 71/145 (48.97%), Query Frame = 0


Query: 240 SFDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALET 299
           +FD DD   +++V + A  RAE FGIP  +    +G+  NI+ A+A+TNAII+    +E 
Sbjct: 335 TFDKDDQLAVEFVTAAANIRAESFGIPLHSLFEAKGIAGNIVHAVATTNAIIAGLIVIEA 394

Query: 300 LKIV-SGCSKTLSNY-LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFID 359
           +K++     K    Y L +   + L   +  +  +  C VC    L+ L+ +    K  D
Sbjct: 395 IKVLKKDVDKFRMTYCLEHPSKKLLLMPIEPYEPNPACYVCSETPLV-LEINTRKSKLRD 454

Query: 360 LLED--HPKLLLSKASVTHRGKNLY 381
           L++     KL ++   + H    LY
Sbjct: 455 LVDKIVKTKLGMNLPLIMHGNSLLY 478

[Close]

BLAST of Chy6G111320 vs. TAIR 10
Match: AT2G21470.3 (SUMO-activating enzyme 2 )

HSP 1 Score: 152.9 bits (385), Expect = 6.1e-37
Identity = 79/203 (38.92%), Postives = 119/203 (58.62%), Query Frame = 0


Query: 44  KVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAK 103
           KVL++GAGG+GCELLK LALSGF ++ +IDMD IEV+NLNRQFLFR   VG+ KA+VA  
Sbjct: 14  KVLMVGAGGIGCELLKTLALSGFEDIHIIDMDTIEVSNLNRQFLFRRSHVGQSKAKVARD 73

Query: 104 RVMERVSGVNILPHFCRIEDKE--IEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSD 163
            V+     +NI  +   +++ E  ++F+  F ++  GLD+++AR ++N +  +       
Sbjct: 74  AVLRFRPNINIRSYHANVKNPEFDVDFFKQFDVVLNGLDNLDARRHVNRLCLA------- 133

Query: 164 DNPLEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAA 223
                    P+V+ GT GF G   V + G T C+EC     P    +P+CT+  TP    
Sbjct: 134 ------ADVPLVESGTTGFLGQVTVHIKGKTECYECQ--TKPAPKTYPVCTITSTPTKFV 193

Query: 224 HCIEYAHLIKWDEVHSGKSFDPD 245
           HCI +A  + + ++   K+ D D
Sbjct: 194 HCIVWAKDLLFAKLFGDKNQDND 201

HSP 2 Score: 55.1 bits (131), Expect = 1.7e-07
Identity = 46/162 (28.40%), Postives = 76/162 (46.91%), Query Frame = 0


Query: 223 CIEYAHLIKWDEVHSGKSFDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIP 282
           C+E  HL          +FD DD   +++V + A  RAE FGIP  +    +G+  NI+ 
Sbjct: 331 CVEIGHL----------TFDKDDQLAVEFVTAAANIRAESFGIPLHSLFEAKGIAGNIVH 390

Query: 283 AIASTNAIISAACALETLKIV-SGCSKTLSNY-LTYNGAEGLHTKVTEFVKDKDCLVCGP 342
           A+A+TNAII+    +E +K++     K    Y L +   + L   +  +  +  C VC  
Sbjct: 391 AVATTNAIIAGLIVIEAIKVLKKDVDKFRMTYCLEHPSKKLLLMPIEPYEPNPACYVCSE 450

Query: 343 GVLIELDSSITLQKFIDLLED--HPKLLLSKASVTHRGKNLY 381
             L+ L+ +    K  DL++     KL ++   + H    LY
Sbjct: 451 TPLV-LEINTRKSKLRDLVDKIVKTKLGMNLPLIMHGNSLLY 481

[Close]

BLAST of Chy6G111320 vs. TAIR 10
Match: AT5G06460.1 (ubiquitin activating enzyme 2 )

HSP 1 Score: 101.3 bits (251), Expect = 2.1e-21
Identity = 68/206 (33.01%), Postives = 110/206 (53.40%), Query Frame = 0


Query: 32  GSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFR-----NLEVIDMDRIEVTNLNRQF 91
           GS L+  L+   +V V+GAG LGCE LK+LAL G        L V D D IE +NL+RQF
Sbjct: 480 GSTLQKKLED-ARVFVVGAGALGCEFLKNLALMGVSCGTQGKLTVTDDDVIEKSNLSRQF 539

Query: 92  LFRLEDVGKPKAEVAAKRVMERVSGVNI--LPHFCRIEDKEI---EFYNDFHIIALGLDS 151
           LFR  ++G+ K+ VAA       S +NI  L +    E + +    F+ +  ++   LD+
Sbjct: 540 LFRDWNIGQAKSTVAATAAAGINSRLNIDALQNRVGPETENVFDDSFWENLTVVVNALDN 599

Query: 152 IEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIWL 211
           + AR Y++S  C + +            KP+++ GT G K + ++++P +T  +  +   
Sbjct: 600 VTARLYVDS-RCVYFQ------------KPLLESGTLGAKCNTQMVIPHLTENYGAS--R 659

Query: 212 FPPQVKFPLCTLAETPRTAAHCIEYA 228
            PP+ + P+CT+   P    HC+ +A
Sbjct: 660 DPPEKQAPMCTVHSFPHNIDHCLTWA 669

The following BLAST results are available for this feature:

BLAST of Chy6G111320 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O65041	1.9e-208	79.55	NEDD8-activating enzyme E1 catalytic subunit OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q9V6U8	2.3e-105	49.50	Nedd8-activating enzyme E1 catalytic subunit OS=Drosophila melanogaster OX=7227 ...	[more]
Q54QG9	8.7e-105	49.14	NEDD8-activating enzyme E1 catalytic subunit OS=Dictyostelium discoideum OX=4468...	[more]
Q8TBC4	3.1e-102	49.12	NEDD8-activating enzyme E1 catalytic subunit OS=Homo sapiens OX=9606 GN=UBA3 PE=...	[more]
Q5R4A0	3.1e-102	48.87	NEDD8-activating enzyme E1 catalytic subunit OS=Pongo abelii OX=9601 GN=UBA3 PE=...	[more]

BLAST of Chy6G111320 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L8U2	7.0e-259	99.55	NEDD8-activating enzyme E1 catalytic subunit OS=Cucumis sativus OX=3659 GN=Csa_3...	[more]
A0A1S3AXQ8	7.8e-258	99.11	NEDD8-activating enzyme E1 catalytic subunit OS=Cucumis melo OX=3656 GN=LOC10348...	[more]
A0A5D3D1R8	2.3e-257	98.89	NEDD8-activating enzyme E1 catalytic subunit OS=Cucumis melo var. makuwa OX=1194...	[more]
A0A6J1CA17	3.4e-253	96.66	NEDD8-activating enzyme E1 catalytic subunit OS=Momordica charantia OX=3673 GN=L...	[more]
A0A6J1C926	4.9e-252	96.86	NEDD8-activating enzyme E1 catalytic subunit OS=Momordica charantia OX=3673 GN=L...	[more]

BLAST of Chy6G111320 vs. NCBI nr


Match Name	Identity	Description
XP_004134149.1	99.55	NEDD8-activating enzyme E1 catalytic subunit [Cucumis sativus] >KGN57012.1 hypot...	[more]
XP_008438709.1	99.11	PREDICTED: NEDD8-activating enzyme E1 catalytic subunit [Cucumis melo]	[more]
KAA0049385.1	98.89	NEDD8-activating enzyme E1 catalytic subunit [Cucumis melo var. makuwa] >TYK1717...	[more]
XP_038897781.1	98.44	NEDD8-activating enzyme E1 catalytic subunit [Benincasa hispida]	[more]
XP_022138082.1	96.66	NEDD8-activating enzyme E1 catalytic subunit isoform X4 [Momordica charantia]	[more]

BLAST of Chy6G111320 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G19180.1	1.3e-209	79.55	E1 C-terminal related 1	[more]
AT2G21470.1	6.1e-37	38.92	SUMO-activating enzyme 2	[more]
AT2G21470.2	6.1e-37	38.92	SUMO-activating enzyme 2	[more]
AT2G21470.3	6.1e-37	38.92	SUMO-activating enzyme 2	[more]
AT5G06460.1	2.1e-21	33.01	ubiquitin activating enzyme 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000011	Ubiquitin/SUMO-activating enzyme E1	PRINTS	PR01849	UBIQUITINACT	coord: 46..70 score: 40.67 coord: 436..449 score: 23.78
IPR014929	E2 binding	SMART	SM01181	E2_bind_2	coord: 344..435 e-value: 5.5E-35 score: 132.2
IPR014929	E2 binding	PFAM	PF08825	E2_bind	coord: 345..434 e-value: 2.4E-21 score: 75.6
IPR000594	THIF-type NAD/FAD binding fold	PFAM	PF00899	ThiF	coord: 36..335 e-value: 5.8E-71 score: 238.7
None	No IPR available	GENE3D	3.40.50.720		coord: 41..342 e-value: 1.1E-65 score: 223.5
None	No IPR available	GENE3D	3.10.290.20		coord: 343..428 e-value: 5.7E-15 score: 57.0
None	No IPR available	PANTHER	PTHR10953	UBIQUITIN-ACTIVATING ENZYME E1	coord: 9..413
None	No IPR available	CDD	cd01488	Uba3_RUB	coord: 44..339 e-value: 0.0 score: 565.829
IPR023318	Ubiquitin activating enzyme, alpha domain superfamily	GENE3D	1.10.10.520	Ubiquitin activating enzymes (Uba3). Chain: B, domain 2	coord: 211..279 e-value: 9.3E-32 score: 110.8
IPR030468	NEDD8-activating enzyme E1 catalytic subunit	PANTHER	PTHR10953:SF6	NEDD8-ACTIVATING ENZYME E1 CATALYTIC SUBUNIT	coord: 9..413
IPR033127	Ubiquitin-activating enzyme E1, Cys active site	PROSITE	PS00865	UBIQUITIN_ACTIVAT_2	coord: 209..217
IPR035985	Ubiquitin-activating enzyme	SUPERFAMILY	69572	Activating enzymes of the ubiquitin-like proteins	coord: 44..402

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy6G111320.1	Chy6G111320.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045116	protein neddylation
biological_process	GO:0006464	cellular protein modification process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0019781	NEDD8 activating enzyme activity
molecular_function	GO:0008641	ubiquitin-like modifier activating enzyme activity