Chy6G111150 (gene) Cucumber (hystrix) v1

Overview
NameChy6G111150
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionthermospermine synthase ACAULIS5-like
LocationchrH06: 3995793 .. 3998345 (+)
RNA-Seq ExpressionChy6G111150
SyntenyChy6G111150
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGATGTTTCCTCTGCTAATTTGGTTAACAATGGGAATGAGCTTATTGAGAAGGGTCATTCCTTAAATGGGTATCGAAAAAGCTGTTGGTATGAAGAAGAAATTGAAGAGAATTTAAGATGGTCCTTTGCTCTTAACAGGTAACTTTTTTTACTTTTCTTTACATTTGATTTCCACACACACACAGAAAAAAGCCCTGTCTAGGTTTTGTTTTTAGTTTTCTGTTTTTGATATTTATGTTTTTTACTTCCAATTTCTCTGTTATGATGTACAACAAGTTTGTTTCGTAAGAACTAAAAACCAAAAACCAAATAGTACTATATTTTAAGTGGGGTTCTTGAGACAAATTTATTTAACGATACTCTCAATGTTCTTAAGCTGTTTGTTGGATATTTTACTGTGGCTATTTTGCTAGACTTTGTGAGGTCCATCAAGTTGAAATAGGGATGAAATCTTTTAAAAAGTGAAACACTTTTTCCCTTTCACTTTAAATCAATAACTCAATAAAATAATCCGTTGGTCTTGGAAAAGCCAGACAAGAAACCTTAAAACACAAAAAAAAAAAAAAAAGAAAAAAGAAAAAAAAAGGATTTTTAGTTTAGGGATTGTTCTTCACTACTTGTCTCACAAAATCTACTTACTTTGTAATCTTGTAGTATACTACATACTGGAGCTTCTCAGTATCAAGATATCGCGTTACTCGACACAAAGCCATTCGGGAAGGCGTTGGTTATCGATGGAAAACTACAGAGTGCAGAAACTGATGAATTCATTTACCATGAATGCCTTGTTCACCCTGCCCTTCTCCATCATCCCAAGTACTTTACAATGCCTAATAGTTAAATTATCTATATAGAATCTTAAGTATAAAATGTTAACTAATATCATGATGTTTTTACTCAGTCCAAAGTCCATTTTTATCATGGGAGGAGGTGAAGGCTCCACAGCAAGAGAACTCCTTAGACATAAGACTGTGGAGAAAGTCGTAATGTGCGACATCGATGAGGTTTAAATTTGTTTCCTTTTCTTCTTTGTTAATCCAATAAAAGATACAAAAAAAATCTCGAAAACCATGATTGATTATTAAGCAAATGAGCAGGAAGTTGTTGATTTCTGCAAGTCATACCTGATAGTCAACAAAGAAGCTTTCTGTGATCCAAGACTTGAACTTATCATTAACGATGCCAGGTTCGGTTTCGATTAGCACATTTAACGATTCTTCTTACGTTACTACGGTTGCTATAAGTTCTGGGGGGTTTATGTTGCAGAGCGGAGCTTGAGAACCGAACTGAGCTATACGACGTAATTGTTGGTGATCTTGCAGACCCAATCGAGGGTGGTCCTTGTTATAAACTCTATACCAAATCTTTCTATGAATATACTGTGAAGCCTAGATTGAAGCAAGATGGCATATTTATTACTCAGGTAAGTGTGAGAGATGCAAATGAAAGAAGTTTGTGAATATAAATCGAGATTGGGAACTGAAGTTGTTGATATCTTTGCAGGCTGGTCCAGCTGGAATTTTCAGCCATACTGAAGTGTTTTCTTGCATCTACAATACTCTAAAGCAAGTTTTTAAATGTGAGAGCAAAACTTTAGGCTATATTATTGTTGCTGACTTTATTCGAAACTTAAAACTTTAAAGAAACTAATGCATTGGCTGGTGGTTTTTTTTTATAATCTGCAGATGTGGTGCCTTATTCTGCCCATATTCCTTCCTTTGCAGATACTTGGGGTTGGGTTATGGTAAGAAAAATGACAACTCTTTTTCATGCTTTTGGGGTTGGAAACCACGGCCTACTTTTTCAACCAATAAACGAGTTTAAAGATCTAGAGACCATTCGACATTTTAAACTAAATCGTGTTTCTAGGTTGCTTTCGAAGCTAGTAGTGCTTATGTACACTTAGAATCACCATTTATTACTATTAAATGGTTGGTTAAGTCCATTGGAATCAAAGTAGAGAAAACAAGCTTACCTTCTAAACTGAAAGAAGTGAAGTTTATGGAAGTTGAGAGTAAATATTGCACACCCGAGGTGTACTGTCGTCGTACATCATCCTGGTTTCAGGGGGTAATTTGGGGCGGGGTGTGATAACAATAATCCGATAACATGTATGTCATTAAGACAACTTTATCAGTTCTAAACTGATAAACTTTCAAATGGGAATCTAGTTTTCTTTTCTTATCATTTGGTTGGAATGTGTTTTAAGGGAGATTTTTATCTTGAACATTGCAGGCCTCAGACACCCCTTTCAATCTGAATGCTGATGATTTGGACCAGAGAATCAAACAAAGAATCACAGGGGAAAACAAGTACTTTGATGGAAAAACTTTCTCATCTGCCTCTACATTAAGCAAAGCCGTTCGGAAGTCGTAAGTAGACGTTTTTCCATTTTGGTTTTGATGGGATTTATTTGAAAACTTTGGAGATGTTAAAGATTGGAAACAATTTATGCAGACTGGACAACGAGACGCAAGTGTACACGGAAGAAACGGCGAGGTTCATATATGGATATGGAGCTGAAGCCTGCAAGCTCAACCAAGTCTGA

mRNA sequence

ATGGCTGATGTTTCCTCTGCTAATTTGGTTAACAATGGGAATGAGCTTATTGAGAAGGGTCATTCCTTAAATGGGTATCGAAAAAGCTGTTGGTATGAAGAAGAAATTGAAGAGAATTTAAGATGGTCCTTTGCTCTTAACAGTATACTACATACTGGAGCTTCTCAGTATCAAGATATCGCGTTACTCGACACAAAGCCATTCGGGAAGGCGTTGGTTATCGATGGAAAACTACAGAGTGCAGAAACTGATGAATTCATTTACCATGAATGCCTTGTTCACCCTGCCCTTCTCCATCATCCCAATCCAAAGTCCATTTTTATCATGGGAGGAGGTGAAGGCTCCACAGCAAGAGAACTCCTTAGACATAAGACTGTGGAGAAAGTCGTAATGTGCGACATCGATGAGGAAGTTGTTGATTTCTGCAAGTCATACCTGATAGTCAACAAAGAAGCTTTCTGTGATCCAAGACTTGAACTTATCATTAACGATGCCAGAGCGGAGCTTGAGAACCGAACTGAGCTATACGACGTAATTGTTGGTGATCTTGCAGACCCAATCGAGGGTGGTCCTTGTTATAAACTCTATACCAAATCTTTCTATGAATATACTGTGAAGCCTAGATTGAAGCAAGATGGCATATTTATTACTCAGGCTGGTCCAGCTGGAATTTTCAGCCATACTGAAGTGTTTTCTTGCATCTACAATACTCTAAAGCAAGTTTTTAAATATGTGGTGCCTTATTCTGCCCATATTCCTTCCTTTGCAGATACTTGGGGTTGGGTTATGGCCTCAGACACCCCTTTCAATCTGAATGCTGATGATTTGGACCAGAGAATCAAACAAAGAATCACAGGGGAAAACAAGTACTTTGATGGAAAAACTTTCTCATCTGCCTCTACATTAAGCAAAGCCGTTCGGAAGTCACTGGACAACGAGACGCAAGTGTACACGGAAGAAACGGCGAGGTTCATATATGGATATGGAGCTGAAGCCTGCAAGCTCAACCAAGTCTGA

Coding sequence (CDS)

ATGGCTGATGTTTCCTCTGCTAATTTGGTTAACAATGGGAATGAGCTTATTGAGAAGGGTCATTCCTTAAATGGGTATCGAAAAAGCTGTTGGTATGAAGAAGAAATTGAAGAGAATTTAAGATGGTCCTTTGCTCTTAACAGTATACTACATACTGGAGCTTCTCAGTATCAAGATATCGCGTTACTCGACACAAAGCCATTCGGGAAGGCGTTGGTTATCGATGGAAAACTACAGAGTGCAGAAACTGATGAATTCATTTACCATGAATGCCTTGTTCACCCTGCCCTTCTCCATCATCCCAATCCAAAGTCCATTTTTATCATGGGAGGAGGTGAAGGCTCCACAGCAAGAGAACTCCTTAGACATAAGACTGTGGAGAAAGTCGTAATGTGCGACATCGATGAGGAAGTTGTTGATTTCTGCAAGTCATACCTGATAGTCAACAAAGAAGCTTTCTGTGATCCAAGACTTGAACTTATCATTAACGATGCCAGAGCGGAGCTTGAGAACCGAACTGAGCTATACGACGTAATTGTTGGTGATCTTGCAGACCCAATCGAGGGTGGTCCTTGTTATAAACTCTATACCAAATCTTTCTATGAATATACTGTGAAGCCTAGATTGAAGCAAGATGGCATATTTATTACTCAGGCTGGTCCAGCTGGAATTTTCAGCCATACTGAAGTGTTTTCTTGCATCTACAATACTCTAAAGCAAGTTTTTAAATATGTGGTGCCTTATTCTGCCCATATTCCTTCCTTTGCAGATACTTGGGGTTGGGTTATGGCCTCAGACACCCCTTTCAATCTGAATGCTGATGATTTGGACCAGAGAATCAAACAAAGAATCACAGGGGAAAACAAGTACTTTGATGGAAAAACTTTCTCATCTGCCTCTACATTAAGCAAAGCCGTTCGGAAGTCACTGGACAACGAGACGCAAGTGTACACGGAAGAAACGGCGAGGTTCATATATGGATATGGAGCTGAAGCCTGCAAGCTCAACCAAGTCTGA

Protein sequence

MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKSIFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIVGDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQVFKYVVPYSAHIPSFADTWGWVMASDTPFNLNADDLDQRIKQRITGENKYFDGKTFSSASTLSKAVRKSLDNETQVYTEETARFIYGYGAEACKLNQV*
Homology
[Close]
BLAST of Chy6G111150 vs. ExPASy Swiss-Prot
Match: Q9S7X6 (Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV=1)

HSP 1 Score: 465.3 bits (1196), Expect = 5.9e-130
Identity = 211/326 (64.72%), Postives = 269/326 (82.52%), Query Frame = 0 

Query: 9   LVNNGNELIEKGHS----LNGYRKSC-WYEEEIEENLRWSFALNSILHTGASQYQDIALL 68
           +  NG   I K  S    L+  ++ C WYEE I+++L+WSFALNS+LH G S+YQDIALL
Sbjct: 8   MFGNGFPEIHKATSPTQTLHSNQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALL 67

Query: 69  DTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKSIFIMGGGEGSTARELLRH 128
           DTK FGK LVIDGK+QSAE DEFIYHECL+HPALL HPNPK++FIMGGGEGS ARE+L+H
Sbjct: 68  DTKRFGKVLVIDGKMQSAERDEFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKH 127

Query: 129 KTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIVGDL 188
            T+EKVVMCDID+EVVDFC+ +L VN +AFC+ +LEL+I DA+AELE R E +D+IVGDL
Sbjct: 128 TTIEKVVMCDIDQEVVDFCRRFLTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDL 187

Query: 189 ADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQVFK 248
           ADP+EGGPCY+LYTKSFY+  +KP+L  +GIF+TQAGPAGIF+H EVF+ IYNT+KQVFK
Sbjct: 188 ADPVEGGPCYQLYTKSFYQNILKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFK 247

Query: 249 YVVPYSAHIPSFADTWGWVMASDTPFNLNADDLDQRIKQRITGENKYFDGKTFSSASTLS 308
           YV  Y+AH+PSFADTWGWVMASD  F++  D++D+RI++R+ GE  Y +  +F SA+TL+
Sbjct: 248 YVKAYTAHVPSFADTWGWVMASDHEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLN 307

Query: 309 KAVRKSLDNETQVYTEETARFIYGYG 330
           K +  +L+ ET+VY+EE ARFI+G+G
Sbjct: 308 KTISLALEKETEVYSEENARFIHGHG 333

[Close]
BLAST of Chy6G111150 vs. ExPASy Swiss-Prot
Match: Q975S5 (Polyamine aminopropyltransferase OS=Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) OX=273063 GN=speE PE=3 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 1.6e-58
Identity = 119/279 (42.65%), Postives = 173/279 (62.01%), Query Frame = 0 

Query: 45  ALNSILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPK 104
           A+  ++    + YQ   L++   FGKAL++DGK+QS  TDEFIYHE LVHP LL   NP+
Sbjct: 20  AITKVIAEEKTPYQRALLVELARFGKALILDGKIQSTITDEFIYHEALVHPLLLSINNPE 79

Query: 105 SIFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIV-NKEAFCDPRLELIIN 164
            I I+GGGEG+T RE+L+HKT++ V M DID  V+DF K YL   ++ AF +P+ +L+I 
Sbjct: 80  KILILGGGEGATLREVLKHKTIKNVTMVDIDPVVIDFAKKYLQEWHQGAFDNPKSKLVIE 139

Query: 165 DARAELENRTELYDVIVGDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAG 224
           D    ++   E +D +V DL DPI+  P   LYTK FYE     R+ + GI      P+ 
Sbjct: 140 DGYKFIKETKEKFDAVVIDLTDPIKDSPSQMLYTKEFYEEV--KRISKWGIVTQATSPS- 199

Query: 225 IFSHTEVFSCIYNTLKQVFKYVVPYSAHIPSFADTWGWVMASD--TPFNLNADDLDQRIK 284
            FS  E FS IYNT+K VFK V     ++P+F   WG+V ASD   P   + ++++ RIK
Sbjct: 200 -FS-LETFSIIYNTIKHVFKKVSAGITYVPAFDGLWGFVYASDEVNPAEFSKEEINNRIK 259

Query: 285 QRITGENKYFDGKTFSSASTLSKAVRKSLDNETQVYTEE 321
           +RI G  +++DG+T  +  ++ K +R+ L+ E ++ T E
Sbjct: 260 ERIDGSLRFYDGETHITMFSIPKHIREVLEKENKISTRE 293

[Close]
BLAST of Chy6G111150 vs. ExPASy Swiss-Prot
Match: A2BJU2 (Polyamine aminopropyltransferase 2 OS=Hyperthermus butylicus (strain DSM 5456 / JCM 9403 / PLM1-5) OX=415426 GN=speE2 PE=1 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 1.9e-56
Identity = 119/280 (42.50%), Postives = 168/280 (60.00%), Query Frame = 0 

Query: 49  ILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKSIFI 108
           I   GA++YQ+  + +    GKALV+DGK+QS+  DE  YHE LVHP LL H  P+ + +
Sbjct: 27  IYAAGATRYQEYMIAELAGIGKALVLDGKVQSSILDEHWYHEALVHPILLAHQCPRKVLV 86

Query: 109 MGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIV-NKEAFCDPRLELIINDARA 168
           +GGGEG+T RE+LRH  VE V M DIDEE+V+  K +L   ++ AF   +LEL+I D R 
Sbjct: 87  IGGGEGATVREVLRHSCVEHVTMVDIDEELVELAKKHLAEWHQGAFSSDKLELVIGDGRR 146

Query: 169 ELENRTELYDVIVGDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSH 228
            +EN    YD I+ DL DP+EGGP  +LYT  FY   VK  L+  G  +TQA    +   
Sbjct: 147 YVENCHRKYDAIILDLVDPMEGGPAARLYTLEFYR-AVKGCLRPGGAVVTQATSPTL--S 206

Query: 229 TEVFSCIYNTLKQVFKYVVPYSAHIPSFADTWGWVMASDT--PFNLNADDLDQRIKQRIT 288
             V++ I NTL +VF  V PY +++ S+   WG+V ASDT  P  L+A ++D+ I  RI 
Sbjct: 207 PRVYAVIRNTLAKVFTIVRPYVSYVRSYNGLWGFVAASDTVDPAKLSAKEVDELIAARIR 266

Query: 289 GENKYFDGKTFSSASTLSKAVRKSLDNETQVYTEETARFI 326
           G+ +++DG+T      L   VR+ L       T+E   ++
Sbjct: 267 GQLRFYDGETHEWMFRLPLPVRQVLSETRDYATDEKPVYV 303

[Close]
BLAST of Chy6G111150 vs. ExPASy Swiss-Prot
Match: Q4JAZ8 (Polyamine aminopropyltransferase OS=Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) OX=330779 GN=speE PE=3 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 3.6e-55
Identity = 117/279 (41.94%), Postives = 167/279 (59.86%), Query Frame = 0 

Query: 45  ALNSILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPK 104
           A+  IL    S++Q + L +   FGKAL+IDGK+QS   DEFIYHE LVHP L+   +P+
Sbjct: 20  AIKRILIEERSEFQRVILAELFRFGKALIIDGKIQSTLADEFIYHESLVHPLLISLEDPE 79

Query: 105 SIFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIV-NKEAFCDPRLELIIN 164
           +I I+GGGEG+T RE LR+K+V KV M DID  V+ F K  L   +  +F D R  LII 
Sbjct: 80  NILILGGGEGATLREALRYKSVRKVTMVDIDPVVIKFAKQNLQEWHMGSFDDSRTNLIIG 139

Query: 165 DARAELENRTELYDVIVGDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAG 224
           D    ++   E Y+ I+ DL DPI+  P   LYT  FY   +K  +  +G  +TQA    
Sbjct: 140 DGYKFVKETNEKYNAIILDLTDPIQDSPSQLLYTSEFYR-DLKSIITPNGGLVTQATSPS 199

Query: 225 IFSHTEVFSCIYNTLKQVFKYVVPYSAHIPSFADTWGWVMASD--TPFNLNADDLDQRIK 284
                + F+ IY+TLK VFK V     ++PSF   WG++ ASD   P +LN + ++ RIK
Sbjct: 200 F--SLDTFAIIYSTLKTVFKNVSAGITYVPSFDGLWGFIYASDVTNPAHLNRNQINDRIK 259

Query: 285 QRITGENKYFDGKTFSSASTLSKAVRKSLDNETQVYTEE 321
           +R+TG  +++DG+T      + K +R+ ++NE +V T E
Sbjct: 260 ERVTGSLRFYDGETHEMLFRVPKYIREKIENEKRVSTRE 295

[Close]
BLAST of Chy6G111150 vs. ExPASy Swiss-Prot
Match: Q9UXE4 (Polyamine aminopropyltransferase OS=Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) OX=273057 GN=speE PE=1 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 3.1e-54
Identity = 113/282 (40.07%), Postives = 167/282 (59.22%), Query Frame = 0 

Query: 46  LNSILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPN-PK 105
           +  +L    + YQ + L++   FGK L+IDGK+QS   DE IYHE LVHP LL     PK
Sbjct: 21  IEKVLAEEKTPYQHVTLVEFTRFGKGLIIDGKVQSTLYDEHIYHELLVHPLLLSLTKPPK 80

Query: 106 SIFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIV-NKEAFCDPRLELIIN 165
           S+ I+GGGEG+T RE+L++K+VEK VM DIDE+V++F K YL   ++ AF D R  L+I 
Sbjct: 81  SVLILGGGEGATLREVLKYKSVEKAVMVDIDEKVIEFAKKYLYEWHQGAFEDKRTNLVIT 140

Query: 166 DARAELENRTELYDVIVGDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAG 225
           D    ++   E YD I+ DL DPI+    Y LYTK FYE  ++  L + G  +TQA    
Sbjct: 141 DGLKFIKETKEKYDAIILDLTDPIKNSTSYMLYTKEFYE-KLREILNERGGIVTQATSPS 200

Query: 226 IFSHTEVFSCIYNTLKQVFKYVVPYSAHIPSFADTWGWVMASDTPFNLNADDLDQRIKQR 285
                EV+  IYNT+K+VFK       ++ SF   WG+V     P  L+ D++D +I +R
Sbjct: 201 F--SLEVYVTIYNTIKEVFKEASASYTYMASFDGLWGFVYGGVRPDLLSEDEVDSKINER 260

Query: 286 ITGENKYFDGKTFSSASTLSKAVRKSLDNETQVYTEETARFI 326
           I G+ +++DG +   + +L K ++      T++ TE+   ++
Sbjct: 261 IDGQLRFYDGYSHKISFSLPKNIKSEFQKITKISTEKDPIYV 299

[Close]
BLAST of Chy6G111150 vs. ExPASy TrEMBL
Match: A0A0A0L7M9 (Spermine synthase OS=Cucumis sativus OX=3659 GN=Csa_3G147770 PE=3 SV=1)

HSP 1 Score: 701.8 bits (1810), Expect = 1.4e-198
Identity = 338/338 (100.00%), Postives = 338/338 (100.00%), Query Frame = 0 

Query: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKSIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKSIFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKSIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFNLNADDLDQRIKQRITGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPFNLNADDLDQRIKQRITGENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFNLNADDLDQRIKQRITGENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDNETQVYTEETARFIYGYGAEACKLNQV 339
           TLSKAVRKSLDNETQVYTEETARFIYGYGAEACKLNQV
Sbjct: 301 TLSKAVRKSLDNETQVYTEETARFIYGYGAEACKLNQV 338

[Close]
BLAST of Chy6G111150 vs. ExPASy TrEMBL
Match: A0A5D3D0P3 (Thermospermine synthase ACAULIS5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00350 PE=3 SV=1)

HSP 1 Score: 700.7 bits (1807), Expect = 3.1e-198
Identity = 337/338 (99.70%), Postives = 338/338 (100.00%), Query Frame = 0 

Query: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSSANLVNNGNELIEKGH+LNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHALNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKSIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKSIFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKSIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFNLNADDLDQRIKQRITGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPFNLNADDLDQRIKQRITGENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFNLNADDLDQRIKQRITGENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDNETQVYTEETARFIYGYGAEACKLNQV 339
           TLSKAVRKSLDNETQVYTEETARFIYGYGAEACKLNQV
Sbjct: 301 TLSKAVRKSLDNETQVYTEETARFIYGYGAEACKLNQV 338

[Close]
BLAST of Chy6G111150 vs. ExPASy TrEMBL
Match: A0A1S3AX06 (thermospermine synthase ACAULIS5-like OS=Cucumis melo OX=3656 GN=LOC103483717 PE=3 SV=1)

HSP 1 Score: 700.7 bits (1807), Expect = 3.1e-198
Identity = 337/338 (99.70%), Postives = 338/338 (100.00%), Query Frame = 0 

Query: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSSANLVNNGNELIEKGH+LNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHALNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKSIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKSIFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKSIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFNLNADDLDQRIKQRITGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPFNLNADDLDQRIKQRITGENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFNLNADDLDQRIKQRITGENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDNETQVYTEETARFIYGYGAEACKLNQV 339
           TLSKAVRKSLDNETQVYTEETARFIYGYGAEACKLNQV
Sbjct: 301 TLSKAVRKSLDNETQVYTEETARFIYGYGAEACKLNQV 338

[Close]
BLAST of Chy6G111150 vs. ExPASy TrEMBL
Match: A0A6J1C9S2 (thermospermine synthase ACAULIS5-like OS=Momordica charantia OX=3673 GN=LOC111009509 PE=3 SV=1)

HSP 1 Score: 675.2 bits (1741), Expect = 1.4e-190
Identity = 325/338 (96.15%), Postives = 329/338 (97.34%), Query Frame = 0 

Query: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSS NLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSVNLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKSIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPK+IFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLEL+INDARAELE+R E YDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELVINDARAELESREEQYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFNLNADDLDQRIKQRITGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMAS+TPF LNADDLDQRIKQRI GENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASNTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDNETQVYTEETARFIYGYGAEACKLNQV 339
           TLSKAVRKSLD ET VYTE TARFIYGYGA ACKLNQV
Sbjct: 301 TLSKAVRKSLDTETHVYTEGTARFIYGYGAAACKLNQV 338

[Close]
BLAST of Chy6G111150 vs. ExPASy TrEMBL
Match: A0A6J1F7M2 (thermospermine synthase ACAULIS5-like OS=Cucurbita moschata OX=3662 GN=LOC111441602 PE=3 SV=1)

HSP 1 Score: 672.9 bits (1735), Expect = 6.8e-190
Identity = 327/339 (96.46%), Postives = 331/339 (97.64%), Query Frame = 0 

Query: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSSANLVNNGNELIEKGH+LNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKSIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHP+LLHHPNPK+IFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPKTIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAF DPRLELIINDARAELENRTE YDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENRTEQYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYE+TVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEHTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFNLNADDLDQRIKQRITGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPF LNADDLDQRIKQRI GENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDNETQVYTEETARFIYGYG-AEACKLNQV 339
           TLSKAVRKSLDNET VYTE TARFIYGYG A ACKLNQV
Sbjct: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV 339

[Close]
BLAST of Chy6G111150 vs. NCBI nr
Match: XP_004134447.1 (thermospermine synthase ACAULIS5 [Cucumis sativus] >KGN56994.1 hypothetical protein Csa_011523 [Cucumis sativus])

HSP 1 Score: 694 bits (1790), Expect = 3.92e-252
Identity = 338/338 (100.00%), Postives = 338/338 (100.00%), Query Frame = 0 

Query: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKSIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKSIFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKSIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFNLNADDLDQRIKQRITGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPFNLNADDLDQRIKQRITGENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFNLNADDLDQRIKQRITGENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDNETQVYTEETARFIYGYGAEACKLNQV 338
           TLSKAVRKSLDNETQVYTEETARFIYGYGAEACKLNQV
Sbjct: 301 TLSKAVRKSLDNETQVYTEETARFIYGYGAEACKLNQV 338

[Close]
BLAST of Chy6G111150 vs. NCBI nr
Match: XP_008438686.1 (PREDICTED: thermospermine synthase ACAULIS5-like [Cucumis melo] >KAA0049369.1 thermospermine synthase ACAULIS5-like [Cucumis melo var. makuwa] >TYK17190.1 thermospermine synthase ACAULIS5-like [Cucumis melo var. makuwa])

HSP 1 Score: 692 bits (1787), Expect = 1.12e-251
Identity = 337/338 (99.70%), Postives = 338/338 (100.00%), Query Frame = 0 

Query: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSSANLVNNGNELIEKGH+LNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHALNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKSIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKSIFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKSIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFNLNADDLDQRIKQRITGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPFNLNADDLDQRIKQRITGENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFNLNADDLDQRIKQRITGENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDNETQVYTEETARFIYGYGAEACKLNQV 338
           TLSKAVRKSLDNETQVYTEETARFIYGYGAEACKLNQV
Sbjct: 301 TLSKAVRKSLDNETQVYTEETARFIYGYGAEACKLNQV 338

[Close]
BLAST of Chy6G111150 vs. NCBI nr
Match: XP_038880186.1 (thermospermine synthase ACAULIS5-like [Benincasa hispida])

HSP 1 Score: 680 bits (1754), Expect = 1.21e-246
Identity = 332/338 (98.22%), Postives = 333/338 (98.52%), Query Frame = 0 

Query: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKSIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPK+IFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFNLNADDLDQRIKQRITGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPF LNADDLDQRIKQRITGENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRITGENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDNETQVYTEETARFIYGYGAEACKLNQV 338
            LSKAVRKSLDNET VYTE TARFIYGYGA ACKLNQV
Sbjct: 301 ILSKAVRKSLDNETHVYTEGTARFIYGYGAAACKLNQV 338

[Close]
BLAST of Chy6G111150 vs. NCBI nr
Match: XP_022138299.1 (thermospermine synthase ACAULIS5-like [Momordica charantia])

HSP 1 Score: 667 bits (1721), Expect = 1.30e-241
Identity = 325/338 (96.15%), Postives = 329/338 (97.34%), Query Frame = 0 

Query: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSS NLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSVNLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKSIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPK+IFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLEL+INDARAELE+R E YDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELVINDARAELESREEQYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFNLNADDLDQRIKQRITGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMAS+TPF LNADDLDQRIKQRI GENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASNTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDNETQVYTEETARFIYGYGAEACKLNQV 338
           TLSKAVRKSLD ET VYTE TARFIYGYGA ACKLNQV
Sbjct: 301 TLSKAVRKSLDTETHVYTEGTARFIYGYGAAACKLNQV 338

[Close]
BLAST of Chy6G111150 vs. NCBI nr
Match: KAG6597450.1 (Thermospermine synthase ACAULIS5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 667 bits (1720), Expect = 1.91e-241
Identity = 328/339 (96.76%), Postives = 331/339 (97.64%), Query Frame = 0 

Query: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSSANLVNNGNELIEKGH+LNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKSIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHP+LLHHPNPK+IFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPKTIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAF DPRLELIINDARAELENRTE YDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENRTEQYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFNLNADDLDQRIKQRITGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPF LNADDLDQRIKQRI GENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDNETQVYTEETARFIYGYGAEA-CKLNQV 338
           TLSKAVRKSLDNET VYTE TARFIYGYGA A CKLNQV
Sbjct: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV 339

[Close]
BLAST of Chy6G111150 vs. TAIR 10
Match: AT5G19530.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 465.3 bits (1196), Expect = 4.2e-131
Identity = 211/326 (64.72%), Postives = 269/326 (82.52%), Query Frame = 0 

Query: 9   LVNNGNELIEKGHS----LNGYRKSC-WYEEEIEENLRWSFALNSILHTGASQYQDIALL 68
           +  NG   I K  S    L+  ++ C WYEE I+++L+WSFALNS+LH G S+YQDIALL
Sbjct: 8   MFGNGFPEIHKATSPTQTLHSNQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALL 67

Query: 69  DTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKSIFIMGGGEGSTARELLRH 128
           DTK FGK LVIDGK+QSAE DEFIYHECL+HPALL HPNPK++FIMGGGEGS ARE+L+H
Sbjct: 68  DTKRFGKVLVIDGKMQSAERDEFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKH 127

Query: 129 KTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIVGDL 188
            T+EKVVMCDID+EVVDFC+ +L VN +AFC+ +LEL+I DA+AELE R E +D+IVGDL
Sbjct: 128 TTIEKVVMCDIDQEVVDFCRRFLTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDL 187

Query: 189 ADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQVFK 248
           ADP+EGGPCY+LYTKSFY+  +KP+L  +GIF+TQAGPAGIF+H EVF+ IYNT+KQVFK
Sbjct: 188 ADPVEGGPCYQLYTKSFYQNILKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFK 247

Query: 249 YVVPYSAHIPSFADTWGWVMASDTPFNLNADDLDQRIKQRITGENKYFDGKTFSSASTLS 308
           YV  Y+AH+PSFADTWGWVMASD  F++  D++D+RI++R+ GE  Y +  +F SA+TL+
Sbjct: 248 YVKAYTAHVPSFADTWGWVMASDHEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLN 307

Query: 309 KAVRKSLDNETQVYTEETARFIYGYG 330
           K +  +L+ ET+VY+EE ARFI+G+G
Sbjct: 308 KTISLALEKETEVYSEENARFIHGHG 333

[Close]
BLAST of Chy6G111150 vs. TAIR 10
Match: AT5G19530.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 465.3 bits (1196), Expect = 4.2e-131
Identity = 211/326 (64.72%), Postives = 269/326 (82.52%), Query Frame = 0 

Query: 9   LVNNGNELIEKGHS----LNGYRKSC-WYEEEIEENLRWSFALNSILHTGASQYQDIALL 68
           +  NG   I K  S    L+  ++ C WYEE I+++L+WSFALNS+LH G S+YQDIALL
Sbjct: 1   MFGNGFPEIHKATSPTQTLHSNQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALL 60

Query: 69  DTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKSIFIMGGGEGSTARELLRH 128
           DTK FGK LVIDGK+QSAE DEFIYHECL+HPALL HPNPK++FIMGGGEGS ARE+L+H
Sbjct: 61  DTKRFGKVLVIDGKMQSAERDEFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKH 120

Query: 129 KTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIVGDL 188
            T+EKVVMCDID+EVVDFC+ +L VN +AFC+ +LEL+I DA+AELE R E +D+IVGDL
Sbjct: 121 TTIEKVVMCDIDQEVVDFCRRFLTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDL 180

Query: 189 ADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQVFK 248
           ADP+EGGPCY+LYTKSFY+  +KP+L  +GIF+TQAGPAGIF+H EVF+ IYNT+KQVFK
Sbjct: 181 ADPVEGGPCYQLYTKSFYQNILKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFK 240

Query: 249 YVVPYSAHIPSFADTWGWVMASDTPFNLNADDLDQRIKQRITGENKYFDGKTFSSASTLS 308
           YV  Y+AH+PSFADTWGWVMASD  F++  D++D+RI++R+ GE  Y +  +F SA+TL+
Sbjct: 241 YVKAYTAHVPSFADTWGWVMASDHEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLN 300

Query: 309 KAVRKSLDNETQVYTEETARFIYGYG 330
           K +  +L+ ET+VY+EE ARFI+G+G
Sbjct: 301 KTISLALEKETEVYSEENARFIHGHG 326

[Close]
BLAST of Chy6G111150 vs. TAIR 10
Match: AT1G23820.2 (spermidine synthase 1 )

HSP 1 Score: 156.4 bits (394), Expect = 4.1e-38
Identity = 80/225 (35.56%), Postives = 128/225 (56.89%), Query Frame = 0 

Query: 43  SFALNSILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPN 102
           S  +  +L  G S YQD+ +  +  +GK LV+DG +Q  E DE  Y E + H  L   PN
Sbjct: 105 SLKVEKVLFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPN 164

Query: 103 PKSIFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELII 162
           PK + ++GGG+G   RE+ RH ++E++ MC+ID+ VVD  K +       + DPR+ L+I
Sbjct: 165 PKKVLVIGGGDGGVLREVARHASIEQIDMCEIDKMVVDVSKQFFPDVAIGYEDPRVNLVI 224

Query: 163 NDARAELENRTE-LYDVIVGDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGP 222
            D  A L+N  E  YD ++ D +DPI  GP  +L+ K F++ +V   L+  G+  TQA  
Sbjct: 225 GDGVAFLKNAAEGSYDAVIVDSSDPI--GPAKELFEKPFFQ-SVARALRPGGVVCTQA-- 284

Query: 223 AGIFSHTEVFSCIYNTLKQVFKYVVPYS-AHIPSFADTWG-WVMA 265
             ++ H ++   I +  +++FK  V Y+   +P++   WG W+ A
Sbjct: 285 ESLWLHMDIIEDIVSNCREIFKGSVNYAWTSVPTYPRHWGHWIYA 324

[Close]
BLAST of Chy6G111150 vs. TAIR 10
Match: AT1G23820.1 (spermidine synthase 1 )

HSP 1 Score: 155.6 bits (392), Expect = 7.1e-38
Identity = 87/278 (31.29%), Postives = 152/278 (54.68%), Query Frame = 0 

Query: 43  SFALNSILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPN 102
           S  +  +L  G S YQD+ +  +  +GK LV+DG +Q  E DE  Y E + H  L   PN
Sbjct: 105 SLKVEKVLFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPN 164

Query: 103 PKSIFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELII 162
           PK + ++GGG+G   RE+ RH ++E++ MC+ID+ VVD  K +       + DPR+ L+I
Sbjct: 165 PKKVLVIGGGDGGVLREVARHASIEQIDMCEIDKMVVDVSKQFFPDVAIGYEDPRVNLVI 224

Query: 163 NDARAELENRTE-LYDVIVGDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGP 222
            D  A L+N  E  YD ++ D +DPI  GP  +L+ K F++ +V   L+  G+  TQA  
Sbjct: 225 GDGVAFLKNAAEGSYDAVIVDSSDPI--GPAKELFEKPFFQ-SVARALRPGGVVCTQA-- 284

Query: 223 AGIFSHTEVFSCIYNTLKQVFKYVVPYS-AHIPSF-ADTWGWVMAS----DTPFNLNADD 282
             ++ H ++   I +  +++FK  V Y+   +P++ +   G+++ S    D  F    + 
Sbjct: 285 ESLWLHMDIIEDIVSNCREIFKGSVNYAWTSVPTYPSGVIGFMLCSTEGPDVDFKHPLNP 344

Query: 283 LDQRIKQRITGENKYFDGKTFSSASTLSKAVRKSLDNE 314
           +D+    +  G  K+++ +  S+A  L    +K ++++
Sbjct: 345 IDES-SSKSNGPLKFYNAEIHSAAFCLPSFAKKVIESK 376

[Close]
BLAST of Chy6G111150 vs. TAIR 10
Match: AT1G70310.1 (spermidine synthase 2 )

HSP 1 Score: 152.5 bits (384), Expect = 6.0e-37
Identity = 93/282 (32.98%), Postives = 149/282 (52.84%), Query Frame = 0 

Query: 43  SFALNSILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPN 102
           S  +  IL  G S YQD+ +  +  +GK LV+DG +Q  E DE  Y E + H  L    N
Sbjct: 65  SLKVEKILFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSISN 124

Query: 103 PKSIFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELII 162
           PK + ++GGG+G   RE+ RH +VE++ +C+ID+ VVD  K Y       + DPR+ LII
Sbjct: 125 PKKVLVIGGGDGGVLREVARHSSVEQIDICEIDKMVVDVAKQYFPNVAVGYEDPRVNLII 184

Query: 163 NDARAELENRTE-LYDVIVGDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGP 222
            D  A L+N  E  YD ++ D +DPI  GP  +L+ K F+E +V   L+  G+  TQA  
Sbjct: 185 GDGVAFLKNAAEGTYDAVIVDSSDPI--GPAKELFEKPFFE-SVNRALRPGGVVCTQA-- 244

Query: 223 AGIFSHTEVFSCIYNTLKQVFKYVVPYS-AHIPSF-ADTWGWVMASD--------TPFNL 282
             ++ H ++   I +  + +FK  V Y+   +P++ +   G+++ S          P +L
Sbjct: 245 ESLWLHMDIIEDIVSNCRDIFKGSVNYAWTSVPTYPSGVIGFMLCSSEGPQVDFKKPVSL 304

Query: 283 NADDLDQRIKQRITGENKYFDGKTFSSASTLSKAVRKSLDNE 314
             D  +  IK       KY++ +  S+A  L    +K +D++
Sbjct: 305 -IDTDESSIKSHC--PLKYYNAEIHSAAFCLPSFAKKVIDSK 338

The following BLAST results are available for this feature:
BLAST of Chy6G111150 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9S7X65.9e-13064.72Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV... [more]
Q975S51.6e-5842.65Polyamine aminopropyltransferase OS=Sulfurisphaera tokodaii (strain DSM 16993 / ... [more]
A2BJU21.9e-5642.50Polyamine aminopropyltransferase 2 OS=Hyperthermus butylicus (strain DSM 5456 / ... [more]
Q4JAZ83.6e-5541.94Polyamine aminopropyltransferase OS=Sulfolobus acidocaldarius (strain ATCC 33909... [more]
Q9UXE43.1e-5440.07Polyamine aminopropyltransferase OS=Saccharolobus solfataricus (strain ATCC 3509... [more]
BLAST of Chy6G111150 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L7M91.4e-198100.00Spermine synthase OS=Cucumis sativus OX=3659 GN=Csa_3G147770 PE=3 SV=1[more]
A0A5D3D0P33.1e-19899.70Thermospermine synthase ACAULIS5-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3AX063.1e-19899.70thermospermine synthase ACAULIS5-like OS=Cucumis melo OX=3656 GN=LOC103483717 PE... [more]
A0A6J1C9S21.4e-19096.15thermospermine synthase ACAULIS5-like OS=Momordica charantia OX=3673 GN=LOC11100... [more]
A0A6J1F7M26.8e-19096.46thermospermine synthase ACAULIS5-like OS=Cucurbita moschata OX=3662 GN=LOC111441... [more]
BLAST of Chy6G111150 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004134447.13.92e-252100.00thermospermine synthase ACAULIS5 [Cucumis sativus] >KGN56994.1 hypothetical prot... [more]
XP_008438686.11.12e-25199.70PREDICTED: thermospermine synthase ACAULIS5-like [Cucumis melo] >KAA0049369.1 th... [more]
XP_038880186.11.21e-24698.22thermospermine synthase ACAULIS5-like [Benincasa hispida][more]
XP_022138299.11.30e-24196.15thermospermine synthase ACAULIS5-like [Momordica charantia][more]
KAG6597450.11.91e-24196.76Thermospermine synthase ACAULIS5, partial [Cucurbita argyrosperma subsp. sororia... [more]
BLAST of Chy6G111150 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G19530.14.2e-13164.72S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G19530.24.2e-13164.72S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G23820.24.1e-3835.56spermidine synthase 1 [more]
AT1G23820.17.1e-3831.29spermidine synthase 1 [more]
AT1G70310.16.0e-3732.98spermidine synthase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR035246Spermidine synthase, tetramerisation domainPFAMPF17284Spermine_synt_Ncoord: 31..82
e-value: 2.5E-13
score: 49.7
NoneNo IPR availablePFAMPF01564Spermine_synthcoord: 85..264
e-value: 1.8E-41
score: 141.7
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 82..333
e-value: 7.2E-78
score: 263.6
NoneNo IPR availablePANTHERPTHR43317THERMOSPERMINE SYNTHASE ACAULIS5coord: 1..331
NoneNo IPR availablePANTHERPTHR43317:SF9SPERMINE SYNTHASEcoord: 1..331
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 110..218
e-value: 1.18328E-5
score: 41.6467
IPR037163Spermidine synthase, tetramerisation domain superfamilyGENE3D2.30.140.10Spermidine synthase, tetramerisation domaincoord: 29..81
e-value: 7.1E-15
score: 56.8
IPR030374Polyamine biosynthesis domainPROSITEPS51006PABS_2coord: 29..266
score: 96.766998
IPR001045Spermidine/spermine synthasesHAMAPMF_00198Spermidine_synthcoord: 30..311
score: 31.244999
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 29..311

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy6G111150.1Chy6G111150.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006596 polyamine biosynthetic process
molecular_function GO:0010487 thermospermine synthase activity
molecular_function GO:0003824 catalytic activity