Chy6G111130 (gene) Cucumber (hystrix) v1

Overview
NameChy6G111130
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionHeat shock 70 kDa protein 8
LocationchrH06: 3985127 .. 3986851 (-)
RNA-Seq ExpressionChy6G111130
SyntenyChy6G111130
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGATTATGGCTGAACCAATATACACCGTAGCTTCTGATAGTGAAACTACTGGGGAAGATAGAACAACATCAACTTTTCCAGAAATTGCAGTTGGAATTGATATTGGTACTTCACAATGTAGTATTGCAGTCTGGAATGGATCTCAGGTAGAGCTTCTTAAGAACACAAGAAACCAGAAAATTATGAGATCATATGTCACCTTCAAGGATGACACTCCTTCTGGGGGAGTTAGTAATGAGCTAAGCCATGAGTATGACATGCTCTCTGGTGCTGCAATTTTCAATATGAAACGATTGATTGGAAGAGTTGACACTGACCCAGTTGTTCATGCATGCAAGAGCCTTCCTTTTCTGGTGCAAACTTTGGACATTGGTGTTCGTCCATTTGTTGCAGCTTTGGTGAATAATGTATGGAGATCTACAACTCCTGAAGAAGTTTTGGCAATTTTCATCGTTGAACTGAAGATGATGGCTGAATATCAACTAAAGCGACCAATTAGAAATGTTGTTTTAACAATTCCAGTTTCATTCAGCCGATTCCAGCTTACTCGTGTGGAACGAGCTTGTGCAATGGCTGGACTTCAAGTTAGATTGATGCCTGAACCAACAGCAGTAGCTTTGTTATATGCTCAACAACAACAGCAGGCTGTACATGACAGCATGGGCAGTGGAAGTGAAAAAATTGCTCTTATTTTCAACATGGGAGCTGGGTATTGTGATGTTGCTGTGTCAGCCATAGGAGGAGGGGTGTCACAGATCAGAGCCCTGGCTGGCAGTCCTATAGGAGGCGAAGATCTACTTCAAAACACCATGAAGTATCTTTTGCCAAATTCTGAATCTATATTCTCACACCGTGGAATTGATGAGATCAGAAGAATGGGGTTGCTCCGAGTTGCGACTCAAGATGCTATCCACAAGCTCTCACTCCAGTCTAGTGTTGAAATCAATGTCAGCTTGGGAAATGGGTCAAAGTTATGCAAGGTTCTTAGCCGAGAGGACTTTGAGATGGTGAACAGCAAGGTGTTCGAGAAATGTGAGAACCTCGTTAAGCAATGCTTACATGATGCACGAGTGGGGATCGAAGATTTGAGTGATGTAGTAGTAGTTGGTGGCTGTTCCTACATTCCAAAGATCAGACATCTTCTGATGGAAATAAGTAAAAAGAAGGCACTTTATGAAGGAATAGATCCTTTAGAAGCTGCAGTTTCTGGTGCAGCTTTAGAAGGTGCAATAGCATCAGGCATTGGCGATCCATTTGGCAGTCTAGATCTTCTTAGCATCCAAGCAACGCCTCTTGCCATTGGAATTCGAGCTGATGGAAACAGTTTCATTCCAATTATCCCCAAGAACACCACAATGCCAGCACGCAAGGAGTTGGTTTTCACAACAATTCAAGACAATCAAAGTGAGGCCCTGATTGTTGTCTATGAAGGCGAAGAGACAAGAGCAGAGGAAAACCATCTGCTTGGCTATTTCAAAATCATTGGAATTCCACCAGCACCCAAAGGAATCCCTGAAATCACAATTTGTATGGACATTGACTCTTCAAATATGCTTAGAGTTCTAGCTGGGGCAACACTCCCAGGTGCTCAACACCCTGCAACTCCTTACATGGAAGTCAAGATGCCGACTGTGGATGATGGACATGGCTGGTGTGCAGAAGCTCTGCATGGAAAATATGGTGCCACCATGGAATTGGTTACATTGCGGAAGAAAAACTAG

mRNA sequence

ATGCAGATTATGGCTGAACCAATATACACCGTAGCTTCTGATAGTGAAACTACTGGGGAAGATAGAACAACATCAACTTTTCCAGAAATTGCAGTTGGAATTGATATTGGTACTTCACAATGTAGTATTGCAGTCTGGAATGGATCTCAGGTAGAGCTTCTTAAGAACACAAGAAACCAGAAAATTATGAGATCATATGTCACCTTCAAGGATGACACTCCTTCTGGGGGAGTTAGTAATGAGCTAAGCCATGAGTATGACATGCTCTCTGGTGCTGCAATTTTCAATATGAAACGATTGATTGGAAGAGTTGACACTGACCCAGTTGTTCATGCATGCAAGAGCCTTCCTTTTCTGGTGCAAACTTTGGACATTGGTGTTCGTCCATTTGTTGCAGCTTTGGTGAATAATGTATGGAGATCTACAACTCCTGAAGAAGTTTTGGCAATTTTCATCGTTGAACTGAAGATGATGGCTGAATATCAACTAAAGCGACCAATTAGAAATGTTGTTTTAACAATTCCAGTTTCATTCAGCCGATTCCAGCTTACTCGTGTGGAACGAGCTTGTGCAATGGCTGGACTTCAAGTTAGATTGATGCCTGAACCAACAGCAGTAGCTTTGTTATATGCTCAACAACAACAGCAGGCTGTACATGACAGCATGGGCAGTGGAAGTGAAAAAATTGCTCTTATTTTCAACATGGGAGCTGGGTATTGTGATGTTGCTGTGTCAGCCATAGGAGGAGGGGTGTCACAGATCAGAGCCCTGGCTGGCAGTCCTATAGGAGGCGAAGATCTACTTCAAAACACCATGAAGTATCTTTTGCCAAATTCTGAATCTATATTCTCACACCGTGGAATTGATGAGATCAGAAGAATGGGGTTGCTCCGAGTTGCGACTCAAGATGCTATCCACAAGCTCTCACTCCAGTCTAGTGTTGAAATCAATGTCAGCTTGGGAAATGGGTCAAAGTTATGCAAGGTTCTTAGCCGAGAGGACTTTGAGATGGTGAACAGCAAGGTGTTCGAGAAATGTGAGAACCTCGTTAAGCAATGCTTACATGATGCACGAGTGGGGATCGAAGATTTGAGTGATGTAGTAGTAGTTGGTGGCTGTTCCTACATTCCAAAGATCAGACATCTTCTGATGGAAATAAGTAAAAAGAAGGCACTTTATGAAGGAATAGATCCTTTAGAAGCTGCAGTTTCTGGTGCAGCTTTAGAAGGTGCAATAGCATCAGGCATTGGCGATCCATTTGGCAGTCTAGATCTTCTTAGCATCCAAGCAACGCCTCTTGCCATTGGAATTCGAGCTGATGGAAACAGTTTCATTCCAATTATCCCCAAGAACACCACAATGCCAGCACGCAAGGAGTTGGTTTTCACAACAATTCAAGACAATCAAAGTGAGGCCCTGATTGTTGTCTATGAAGGCGAAGAGACAAGAGCAGAGGAAAACCATCTGCTTGGCTATTTCAAAATCATTGGAATTCCACCAGCACCCAAAGGAATCCCTGAAATCACAATTTGTATGGACATTGACTCTTCAAATATGCTTAGAGTTCTAGCTGGGGCAACACTCCCAGGTGCTCAACACCCTGCAACTCCTTACATGGAAGTCAAGATGCCGACTGTGGATGATGGACATGGCTGGTGTGCAGAAGCTCTGCATGGAAAATATGGTGCCACCATGGAATTGGTTACATTGCGGAAGAAAAACTAG

Coding sequence (CDS)

ATGCAGATTATGGCTGAACCAATATACACCGTAGCTTCTGATAGTGAAACTACTGGGGAAGATAGAACAACATCAACTTTTCCAGAAATTGCAGTTGGAATTGATATTGGTACTTCACAATGTAGTATTGCAGTCTGGAATGGATCTCAGGTAGAGCTTCTTAAGAACACAAGAAACCAGAAAATTATGAGATCATATGTCACCTTCAAGGATGACACTCCTTCTGGGGGAGTTAGTAATGAGCTAAGCCATGAGTATGACATGCTCTCTGGTGCTGCAATTTTCAATATGAAACGATTGATTGGAAGAGTTGACACTGACCCAGTTGTTCATGCATGCAAGAGCCTTCCTTTTCTGGTGCAAACTTTGGACATTGGTGTTCGTCCATTTGTTGCAGCTTTGGTGAATAATGTATGGAGATCTACAACTCCTGAAGAAGTTTTGGCAATTTTCATCGTTGAACTGAAGATGATGGCTGAATATCAACTAAAGCGACCAATTAGAAATGTTGTTTTAACAATTCCAGTTTCATTCAGCCGATTCCAGCTTACTCGTGTGGAACGAGCTTGTGCAATGGCTGGACTTCAAGTTAGATTGATGCCTGAACCAACAGCAGTAGCTTTGTTATATGCTCAACAACAACAGCAGGCTGTACATGACAGCATGGGCAGTGGAAGTGAAAAAATTGCTCTTATTTTCAACATGGGAGCTGGGTATTGTGATGTTGCTGTGTCAGCCATAGGAGGAGGGGTGTCACAGATCAGAGCCCTGGCTGGCAGTCCTATAGGAGGCGAAGATCTACTTCAAAACACCATGAAGTATCTTTTGCCAAATTCTGAATCTATATTCTCACACCGTGGAATTGATGAGATCAGAAGAATGGGGTTGCTCCGAGTTGCGACTCAAGATGCTATCCACAAGCTCTCACTCCAGTCTAGTGTTGAAATCAATGTCAGCTTGGGAAATGGGTCAAAGTTATGCAAGGTTCTTAGCCGAGAGGACTTTGAGATGGTGAACAGCAAGGTGTTCGAGAAATGTGAGAACCTCGTTAAGCAATGCTTACATGATGCACGAGTGGGGATCGAAGATTTGAGTGATGTAGTAGTAGTTGGTGGCTGTTCCTACATTCCAAAGATCAGACATCTTCTGATGGAAATAAGTAAAAAGAAGGCACTTTATGAAGGAATAGATCCTTTAGAAGCTGCAGTTTCTGGTGCAGCTTTAGAAGGTGCAATAGCATCAGGCATTGGCGATCCATTTGGCAGTCTAGATCTTCTTAGCATCCAAGCAACGCCTCTTGCCATTGGAATTCGAGCTGATGGAAACAGTTTCATTCCAATTATCCCCAAGAACACCACAATGCCAGCACGCAAGGAGTTGGTTTTCACAACAATTCAAGACAATCAAAGTGAGGCCCTGATTGTTGTCTATGAAGGCGAAGAGACAAGAGCAGAGGAAAACCATCTGCTTGGCTATTTCAAAATCATTGGAATTCCACCAGCACCCAAAGGAATCCCTGAAATCACAATTTGTATGGACATTGACTCTTCAAATATGCTTAGAGTTCTAGCTGGGGCAACACTCCCAGGTGCTCAACACCCTGCAACTCCTTACATGGAAGTCAAGATGCCGACTGTGGATGATGGACATGGCTGGTGTGCAGAAGCTCTGCATGGAAAATATGGTGCCACCATGGAATTGGTTACATTGCGGAAGAAAAACTAG

Protein sequence

MQIMAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIMRSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTLDIGVRPFVAALVNNVWRSTTPEEVLAIFIVELKMMAEYQLKRPIRNVVLTIPVSFSRFQLTRVERACAMAGLQVRLMPEPTAVALLYAQQQQQAVHDSMGSGSEKIALIFNMGAGYCDVAVSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQDAIHKLSLQSSVEINVSLGNGSKLCKVLSREDFEMVNSKVFEKCENLVKQCLHDARVGIEDLSDVVVVGGCSYIPKIRHLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSLDLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTIQDNQSEALIVVYEGEETRAEENHLLGYFKIIGIPPAPKGIPEITICMDIDSSNMLRVLAGATLPGAQHPATPYMEVKMPTVDDGHGWCAEALHGKYGATMELVTLRKKN*
Homology
BLAST of Chy6G111130 vs. ExPASy Swiss-Prot
Match: Q9SKY8 (Heat shock 70 kDa protein 8 OS=Arabidopsis thaliana OX=3702 GN=HSP70-8 PE=1 SV=1)

HSP 1 Score: 835.9 bits (2158), Expect = 2.8e-241
Identity = 404/573 (70.51%), Postives = 491/573 (85.69%), Query Frame = 0

Query: 4   MAEPIYTVASDSETTGEDRTTS--TFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQK 63
           MAE  YTVASDSE TGE++++S  + PEIA+GIDIGTSQCSIAVWNGSQV +L+NTRNQK
Sbjct: 1   MAEAAYTVASDSENTGEEKSSSSPSLPEIALGIDIGTSQCSIAVWNGSQVHILRNTRNQK 60

Query: 64  IMRSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQ 123
           +++S+VTFKD+ P+GGVSN+L+HE +ML+GAAIFNMKRL+GRVDTDPVVHA K+LPFLVQ
Sbjct: 61  LIKSFVTFKDEVPAGGVSNQLAHEQEMLTGAAIFNMKRLVGRVDTDPVVHASKNLPFLVQ 120

Query: 124 TLDIGVRPFVAALVNNVWRSTTPEEVLAIFIVELKMMAEYQLKRPIRNVVLTIPVSFSRF 183
           TLDIGVRPF+AALVNN WRSTTPEEVLAIF+VEL++MAE QLKRP+RNVVLT+PVSFSRF
Sbjct: 121 TLDIGVRPFIAALVNNAWRSTTPEEVLAIFLVELRLMAEAQLKRPVRNVVLTVPVSFSRF 180

Query: 184 QLTRVERACAMAGLQV-RLMPEPTAVALLYAQQQQQAVHDSMGSGSEKIALIFNMGAGYC 243
           QLTR ERACAMAGL V RLMPEPTA+ALLYAQQQQ   HD+MGSGSE++A+IFNMGAGYC
Sbjct: 181 QLTRFERACAMAGLHVLRLMPEPTAIALLYAQQQQMTTHDNMGSGSERLAVIFNMGAGYC 240

Query: 244 DVAVSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVA 303
           DVAV+A  GGVSQI+ALAGSPIGGED+LQNT++++ P +E              GLLRVA
Sbjct: 241 DVAVTATAGGVSQIKALAGSPIGGEDILQNTIRHIAPPNE-----------EASGLLRVA 300

Query: 304 TQDAIHKLSLQSSVEINVSLGNGSKLCKVLSREDFEMVNSKVFEKCENLVKQCLHDARVG 363
            QDAIH+L+ Q +V+I V LGNG+K+ KVL R +FE VN KVFE+CE LV QCL DARV 
Sbjct: 301 AQDAIHRLTDQENVQIEVDLGNGNKISKVLDRLEFEEVNQKVFEECERLVVQCLRDARVN 360

Query: 364 IEDLSDVVVVGGCSYIPKIRHLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPF 423
             D+ D+++VGGCSYIPK+R ++  + KK  +Y+G++PLEAAV GAALEGA+ SGI DPF
Sbjct: 361 GGDIDDLIMVGGCSYIPKVRTIIKNVCKKDEIYKGVNPLEAAVRGAALEGAVTSGIHDPF 420

Query: 424 GSLDLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTIQDNQSEALIVVYEGE 483
           GSLDLL+IQATPLA+G+RA+GN FIP+IP+NT +PARK+L FTT+QDNQ EALI++YEGE
Sbjct: 421 GSLDLLTIQATPLAVGVRANGNKFIPVIPRNTMVPARKDLFFTTVQDNQKEALIIIYEGE 480

Query: 484 ETRAEENHLLGYFKIIGIPPAPKGIPEITICMDIDSSNMLRVLAGATLPGAQHPATPYME 543
               EENHLLGYFK++GIPPAPKG+PEI +CMDID+SN LRV A   +PG+  P  P +E
Sbjct: 481 GETVEENHLLGYFKLVGIPPAPKGVPEINVCMDIDASNALRVFAAVLMPGSSSPVVPVIE 540

Query: 544 VKMPTVDDGHGWCAEALHGKYGATMELVTLRKK 574
           V+MPTVDDGHGWCA+AL+ KYGAT++L+TL++K
Sbjct: 541 VRMPTVDDGHGWCAQALNVKYGATLDLITLQRK 562

BLAST of Chy6G111130 vs. ExPASy Swiss-Prot
Match: Q9LHA8 (Heat shock 70 kDa protein 4 OS=Arabidopsis thaliana OX=3702 GN=HSP70-4 PE=1 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 5.7e-77
Identity = 180/511 (35.23%), Postives = 287/511 (56.16%), Query Frame = 0

Query: 31  AVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIMRSYVTFKDDTPSGGVSNELSHEYDMLS 90
           A+GID+GT+   + VW   +VE++ N +  +   SYV F D      + +   ++  M  
Sbjct: 9   AIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERL--IGDAAKNQVAMNP 68

Query: 91  GAAIFNMKRLIGRVDTDPVVHACKS-LPFLVQTLDIGVRPFVAALVNNVWRSTTPEEVLA 150
              +F+ KRLIGR  +DP V A KS  PF V +   G +P +        +  + EE+ +
Sbjct: 69  TNTVFDAKRLIGRRYSDPSVQADKSHWPFKVVS-GPGEKPMIVVNHKGEEKQFSAEEISS 128

Query: 151 IFIVELKMMAEYQLKRPIRNVVLTIPVSFSRFQLTRVERACAMAGLQV-RLMPEPTAVAL 210
           + +++++ +AE  L  P++N V+T+P  F+  Q    + A  ++GL V R++ EPTA A+
Sbjct: 129 MVLIKMREIAEAFLGSPVKNAVVTVPAYFNDSQRQATKDAGVISGLNVMRIINEPTAAAI 188

Query: 211 LYAQQQQQAVHDSMGSGSEKIALIFNMGAGYCDVAVSAIGGGVSQIRALAG-SPIGGEDL 270
            Y   ++ +      S  EK  LIF++G G  DV++  I  G+ +++A AG + +GGED 
Sbjct: 189 AYGLDKKAS------SVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDF 248

Query: 271 LQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQDAIHKLS--LQSSVEINVSLGNGSK 330
               + + +   +           R +  LR A + A   LS   Q+++EI+ SL  G  
Sbjct: 249 DNRMVNHFVQEFKRKNKKDITGNPRALRRLRTACERAKRTLSSTAQTTIEID-SLFEGID 308

Query: 331 LCKVLSREDFEMVNSKVFEKCENLVKQCLHDARVGIEDLSDVVVVGGCSYIPKIRHLLME 390
               ++R  FE +N  +F KC   V++CL DA++    + DVV+VGG + IPK++ LL +
Sbjct: 309 FYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQD 368

Query: 391 ISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSLDLLSIQATPLAIGIRADGNSFI 450
               K L + I+P EA   GAA++ AI SG G+     DLL +  TPL++G+   G    
Sbjct: 369 FFNGKELCKSINPDEAVAYGAAVQAAILSGEGNE-KVQDLLLLDVTPLSLGLETAGGVMT 428

Query: 451 PIIPKNTTMPARKELVFTTIQDNQSEALIVVYEGEETRAEENHLLGYFKIIGIPPAPKGI 510
            +IP+NTT+P +KE +F+T  DNQ   LI VYEGE  R ++N+LLG F++ GIPPAP+G+
Sbjct: 429 VLIPRNTTIPTKKEQIFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPAPRGV 488

Query: 511 PEITICMDIDSSNMLRVLAGATLPGAQHPAT 537
           P+IT+C DID++ +L V A     G ++  T
Sbjct: 489 PQITVCFDIDANGILNVSAEDKTTGQKNKIT 508

BLAST of Chy6G111130 vs. ExPASy Swiss-Prot
Match: Q9S9N1 (Heat shock 70 kDa protein 5 OS=Arabidopsis thaliana OX=3702 GN=HSP70-5 PE=2 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 4.8e-76
Identity = 182/517 (35.20%), Postives = 284/517 (54.93%), Query Frame = 0

Query: 25  STFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIMRSYVTFKDDTPSGGVSNELSH 84
           +T  E A+GID+GT+   + VW   +VE++ N +  +   SYV F D      + +   +
Sbjct: 2   ATKSEKAIGIDLGTTYSCVGVWMNDRVEIIPNDQGNRTTPSYVAFTDTERL--IGDAAKN 61

Query: 85  EYDMLSGAAIFNMKRLIGRVDTDPVVHA-CKSLPFLVQTLDIGVRPFVAALVNNVWRSTT 144
           +  +     +F+ KRLIGR  +DP V +     PF V +   G +P +     N  +  +
Sbjct: 62  QVALNPQNTVFDAKRLIGRKFSDPSVQSDILHWPFKVVS-GPGEKPMIVVSYKNEEKQFS 121

Query: 145 PEEVLAIFIVELKMMAEYQLKRPIRNVVLTIPVSFSRFQLTRVERACAMAGLQV-RLMPE 204
           PEE+ ++ +V++K +AE  L R ++N V+T+P  F+  Q    + A A++GL V R++ E
Sbjct: 122 PEEISSMVLVKMKEVAEAFLGRTVKNAVVTVPAYFNDSQRQATKDAGAISGLNVLRIINE 181

Query: 205 PTAVALLYAQQQQQAVHDSMGSGSEKIALIFNMGAGYCDVAVSAIGGGVSQIRALAG-SP 264
           PTA A+ Y   ++           EK  LIF++G G  DV++  I  GV +++A AG + 
Sbjct: 182 PTAAAIAYGLDKKGT------KAGEKNVLIFDLGGGTFDVSLLTIEEGVFEVKATAGDTH 241

Query: 265 IGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQDAIHKLS--LQSSVEINVS 324
           +GGED     + + +               R +  LR A + A   LS   Q+++EI+ S
Sbjct: 242 LGGEDFDNRLVNHFVAEFRRKHKKDIAGNARALRRLRTACERAKRTLSSTAQTTIEID-S 301

Query: 325 LGNGSKLCKVLSREDFEMVNSKVFEKCENLVKQCLHDARVGIEDLSDVVVVGGCSYIPKI 384
           L  G      +SR  FE +N  +F KC + V++ L DA++    + DVV+VGG + IPKI
Sbjct: 302 LHEGIDFYATISRARFEEMNMDLFRKCMDPVEKVLKDAKLDKSSVHDVVLVGGSTRIPKI 361

Query: 385 RHLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSLDLLSIQATPLAIGIRA 444
           + LL +    K L + I+P EA   GAA++ AI +G G      DLL +   PL++G+  
Sbjct: 362 QQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILTGEGSE-KVQDLLLLDVAPLSLGLET 421

Query: 445 DGNSFIPIIPKNTTMPARKELVFTTIQDNQSEALIVVYEGEETRAEENHLLGYFKIIGIP 504
            G     +IP+NTT+P +KE VF+T  DNQ   LI VYEGE  R  +N+LLG F++ GIP
Sbjct: 422 AGGVMTVLIPRNTTVPCKKEQVFSTYADNQPGVLIQVYEGERARTRDNNLLGTFELKGIP 481

Query: 505 PAPKGIPEITICMDIDSSNMLRVLAGATLPGAQHPAT 537
           PAP+G+P+I +C DID++ +L V A     G ++  T
Sbjct: 482 PAPRGVPQINVCFDIDANGILNVSAEDKTAGVKNQIT 507

BLAST of Chy6G111130 vs. ExPASy Swiss-Prot
Match: P09189 (Heat shock cognate 70 kDa protein OS=Petunia hybrida OX=4102 GN=HSP70 PE=2 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 6.3e-76
Identity = 183/515 (35.53%), Postives = 285/515 (55.34%), Query Frame = 0

Query: 31  AVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIMRSYVTFKDDTPSGGVSNELSHEYDMLS 90
           A+GID+GT+   + VW   +VE++ N +  +   SYV F D      + +   ++  M  
Sbjct: 9   AIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERL--IGDAAKNQVAMNP 68

Query: 91  GAAIFNMKRLIGRVDTDPVVHA-CKSLPFLVQTLDIGVRPFVAALVNNVWRSTTPEEVLA 150
              +F+ KRLIGR  +DP V +  K  PF V     G +P +        +    EE+ +
Sbjct: 69  INTVFDAKRLIGRRFSDPSVQSDIKLWPFKV-IPGPGDKPMIVVTYKGEEKQFAAEEISS 128

Query: 151 IFIVELKMMAEYQLKRPIRNVVLTIPVSFSRFQLTRVERACAMAGLQV-RLMPEPTAVAL 210
           + + ++K +AE  L   I+N V+T+P  F+  Q    + A  +AGL V R++ EPTA A+
Sbjct: 129 MVLTKMKEIAEAYLGTTIKNAVVTVPAYFNDSQRQATKDAGVIAGLNVMRIINEPTAAAI 188

Query: 211 LYAQQQQQAVHDSMGSGSEKIALIFNMGAGYCDVAVSAIGGGVSQIRALAG-SPIGGEDL 270
            Y   ++ +      S  EK  LIF++G G  DV++  I  G+ +++A AG + +GGED 
Sbjct: 189 AYGLDKKAS------SAGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDF 248

Query: 271 LQNTMKYLLPNSESIFSHRGIDEI----RRMGLLRVATQDAIHKLS--LQSSVEINVSLG 330
               + + +      F  +   +I    R +  LR A + A   LS   Q+++EI+ SL 
Sbjct: 249 DNRMVNHFVQE----FKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEID-SLY 308

Query: 331 NGSKLCKVLSREDFEMVNSKVFEKCENLVKQCLHDARVGIEDLSDVVVVGGCSYIPKIRH 390
            G      ++R  FE +N  +F KC   V++CL DA++    + DVV+VGG + IPK++ 
Sbjct: 309 EGIDFYSTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQ 368

Query: 391 LLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSLDLLSIQATPLAIGIRADG 450
           LL +    K L + I+P EA   GAA++ AI SG G+     DLL +  TPL++G+   G
Sbjct: 369 LLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNE-KVQDLLLLDVTPLSLGLETAG 428

Query: 451 NSFIPIIPKNTTMPARKELVFTTIQDNQSEALIVVYEGEETRAEENHLLGYFKIIGIPPA 510
                +IP+NTT+P +KE VF+T  DNQ   LI VYEGE  R ++N+LLG F++ GIPPA
Sbjct: 429 GGMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPA 488

Query: 511 PKGIPEITICMDIDSSNMLRVLAGATLPGAQHPAT 537
           P+G+P+IT+C DID++ +L V A     G ++  T
Sbjct: 489 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKIT 508

BLAST of Chy6G111130 vs. ExPASy Swiss-Prot
Match: P22953 (Heat shock 70 kDa protein 1 OS=Arabidopsis thaliana OX=3702 GN=HSP70-1 PE=1 SV=3)

HSP 1 Score: 283.1 bits (723), Expect = 7.0e-75
Identity = 178/511 (34.83%), Postives = 282/511 (55.19%), Query Frame = 0

Query: 31  AVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIMRSYVTFKDDTPSGGVSNELSHEYDMLS 90
           A+GID+GT+   + VW   +VE++ N +  +   SYV F D      + +   ++  M  
Sbjct: 9   AIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERL--IGDAAKNQVAMNP 68

Query: 91  GAAIFNMKRLIGRVDTDPVVHA-CKSLPFLVQTLDIGVRPFVAALVNNVWRSTTPEEVLA 150
              +F+ KRLIGR  +D  V +  K  PF +Q      +P +        +    EE+ +
Sbjct: 69  VNTVFDAKRLIGRRFSDSSVQSDMKLWPFKIQA-GPADKPMIYVEYKGEEKEFAAEEISS 128

Query: 151 IFIVELKMMAEYQLKRPIRNVVLTIPVSFSRFQLTRVERACAMAGLQV-RLMPEPTAVAL 210
           + +++++ +AE  L   I+N V+T+P  F+  Q    + A  +AGL V R++ EPTA A+
Sbjct: 129 MVLIKMREIAEAYLGVTIKNAVVTVPAYFNDSQRQATKDAGVIAGLNVMRIINEPTAAAI 188

Query: 211 LYAQQQQQAVHDSMGSGSEKIALIFNMGAGYCDVAVSAIGGGVSQIRALAG-SPIGGEDL 270
            Y   ++        S  EK  LIF++G G  DV++  I  G+ +++A AG + +GGED 
Sbjct: 189 AYGLDKKAT------SVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDF 248

Query: 271 LQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQDAIHKLS--LQSSVEINVSLGNGSK 330
               + + +   +           R +  LR + + A   LS   Q+++EI+ SL  G  
Sbjct: 249 DNRMVNHFVQEFKRKSKKDITGNPRALRRLRTSCERAKRTLSSTAQTTIEID-SLYEGID 308

Query: 331 LCKVLSREDFEMVNSKVFEKCENLVKQCLHDARVGIEDLSDVVVVGGCSYIPKIRHLLME 390
               ++R  FE +N  +F KC   V++CL DA++    + DVV+VGG + IPK++ LL +
Sbjct: 309 FYSTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQQLLQD 368

Query: 391 ISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSLDLLSIQATPLAIGIRADGNSFI 450
               K L + I+P EA   GAA++GAI SG G+     DLL +  TPL++G+   G    
Sbjct: 369 FFNGKELCKSINPDEAVAYGAAVQGAILSGEGNE-KVQDLLLLDVTPLSLGLETAGGVMT 428

Query: 451 PIIPKNTTMPARKELVFTTIQDNQSEALIVVYEGEETRAEENHLLGYFKIIGIPPAPKGI 510
            +IP+NTT+P +KE VF+T  DNQ   LI VYEGE  R ++N+LLG F++ GIPPAP+G+
Sbjct: 429 TLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPAPRGV 488

Query: 511 PEITICMDIDSSNMLRVLAGATLPGAQHPAT 537
           P+IT+C DID++ +L V A     G ++  T
Sbjct: 489 PQITVCFDIDANGILNVSAEDKTTGQKNKIT 508

BLAST of Chy6G111130 vs. ExPASy TrEMBL
Match: A0A0A0LAC6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G147740 PE=3 SV=1)

HSP 1 Score: 1117.8 bits (2890), Expect = 0.0e+00
Identity = 567/571 (99.30%), Postives = 570/571 (99.82%), Query Frame = 0

Query: 4   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 63
           MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM
Sbjct: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60

Query: 64  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 123
           RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120

Query: 124 DIGVRPFVAALVNNVWRSTTPEEVLAIFIVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 183
           DIGVRPFVAALVNNVWRSTTPEEVLAIFIVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFIVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 184 TRVERACAMAGLQVRLMPEPTAVALLYAQQQQQAVHDSMGSGSEKIALIFNMGAGYCDVA 243
           TRVERACAMAGLQVRLMPEPTAVALLYAQQQQQAVHDSMGSGSEKIALIFNMGAGYCDVA
Sbjct: 181 TRVERACAMAGLQVRLMPEPTAVALLYAQQQQQAVHDSMGSGSEKIALIFNMGAGYCDVA 240

Query: 244 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 303
           VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD
Sbjct: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300

Query: 304 AIHKLSLQSSVEINVSLGNGSKLCKVLSREDFEMVNSKVFEKCENLVKQCLHDARVGIED 363
           AIHKLS QSSVEINVSLGNGSKLCKVLSREDFEMVNSKVFEKCENLVKQCLHDAR+GIED
Sbjct: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREDFEMVNSKVFEKCENLVKQCLHDARLGIED 360

Query: 364 LSDVVVVGGCSYIPKIRHLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 423
           LSDVVVVGGCSYIPKIRHLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL
Sbjct: 361 LSDVVVVGGCSYIPKIRHLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420

Query: 424 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTIQDNQSEALIVVYEGEETR 483
           DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTIQDNQSEALIVVYEGEETR
Sbjct: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTIQDNQSEALIVVYEGEETR 480

Query: 484 AEENHLLGYFKIIGIPPAPKGIPEITICMDIDSSNMLRVLAGATLPGAQHPATPYMEVKM 543
           AEENHLLGYFKIIGIPPAPKG+PEITICMDIDSSNMLRVLAGATLPGAQHPATPYMEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGAQHPATPYMEVKM 540

Query: 544 PTVDDGHGWCAEALHGKYGATMELVTLRKKN 575
           PTVDDGHGWCAEALHGKYGATMELVTLRKK+
Sbjct: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKKS 571

BLAST of Chy6G111130 vs. ExPASy TrEMBL
Match: A0A5D3D052 (Heat shock 70 kDa protein 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00370 PE=3 SV=1)

HSP 1 Score: 1102.8 bits (2851), Expect = 0.0e+00
Identity = 559/571 (97.90%), Postives = 565/571 (98.95%), Query Frame = 0

Query: 4   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 63
           MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGS VELLKNTR+QKIM
Sbjct: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIM 60

Query: 64  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 123
           RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120

Query: 124 DIGVRPFVAALVNNVWRSTTPEEVLAIFIVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 183
           DIGVRPFVAALVNNVWRSTTPEEVLAIF+VELKMMAEYQLKRPIRNVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 184 TRVERACAMAGLQVRLMPEPTAVALLYAQQQQQAVHDSMGSGSEKIALIFNMGAGYCDVA 243
           TRVERACAMAG QVRLMPEPTAVALLYAQQQQQA HDSMGSGSEKIALIFNMGAGYCDVA
Sbjct: 181 TRVERACAMAGFQVRLMPEPTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDVA 240

Query: 244 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 303
           VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD
Sbjct: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300

Query: 304 AIHKLSLQSSVEINVSLGNGSKLCKVLSREDFEMVNSKVFEKCENLVKQCLHDARVGIED 363
           AIHKLS QSSVEINVSLGNGSKLCKVLSRE+FEMVNSKVFEKCENLVKQCLHDARVGIED
Sbjct: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIED 360

Query: 364 LSDVVVVGGCSYIPKIRHLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 423
           LSDVVVVGGCSYIPKIR LLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL
Sbjct: 361 LSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420

Query: 424 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTIQDNQSEALIVVYEGEETR 483
           DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTT+QDNQSEALIVVYEGEETR
Sbjct: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETR 480

Query: 484 AEENHLLGYFKIIGIPPAPKGIPEITICMDIDSSNMLRVLAGATLPGAQHPATPYMEVKM 543
           AEENHLLGYFKIIGIPPAPKG+PEITICMDIDSSNMLRVLAGA LPGAQHPATPYMEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYMEVKM 540

Query: 544 PTVDDGHGWCAEALHGKYGATMELVTLRKKN 575
           PTVDDGHGWCAEALHGKYGATMELVTLRKK+
Sbjct: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKKS 571

BLAST of Chy6G111130 vs. ExPASy TrEMBL
Match: A0A1S3AXP0 (heat shock 70 kDa protein 8 OS=Cucumis melo OX=3656 GN=LOC103483715 PE=3 SV=1)

HSP 1 Score: 1101.3 bits (2847), Expect = 0.0e+00
Identity = 558/571 (97.72%), Postives = 564/571 (98.77%), Query Frame = 0

Query: 4   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 63
           MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGS VELLKNTR+QKIM
Sbjct: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIM 60

Query: 64  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 123
           RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120

Query: 124 DIGVRPFVAALVNNVWRSTTPEEVLAIFIVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 183
           DIGVRPFVAALVNNVWRSTTPEEVLAIF+VELKMMAEYQLKRPIRNVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 184 TRVERACAMAGLQVRLMPEPTAVALLYAQQQQQAVHDSMGSGSEKIALIFNMGAGYCDVA 243
           TRVERACAMAG QVRLMPEPTAVALLYAQQQQQA HDSMGSGSEKIALIFNMGAGYCD A
Sbjct: 181 TRVERACAMAGFQVRLMPEPTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDAA 240

Query: 244 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 303
           VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD
Sbjct: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300

Query: 304 AIHKLSLQSSVEINVSLGNGSKLCKVLSREDFEMVNSKVFEKCENLVKQCLHDARVGIED 363
           AIHKLS QSSVEINVSLGNGSKLCKVLSRE+FEMVNSKVFEKCENLVKQCLHDARVGIED
Sbjct: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIED 360

Query: 364 LSDVVVVGGCSYIPKIRHLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 423
           LSDVVVVGGCSYIPKIR LLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL
Sbjct: 361 LSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420

Query: 424 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTIQDNQSEALIVVYEGEETR 483
           DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTT+QDNQSEALIVVYEGEETR
Sbjct: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETR 480

Query: 484 AEENHLLGYFKIIGIPPAPKGIPEITICMDIDSSNMLRVLAGATLPGAQHPATPYMEVKM 543
           AEENHLLGYFKIIGIPPAPKG+PEITICMDIDSSNMLRVLAGA LPGAQHPATPYMEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYMEVKM 540

Query: 544 PTVDDGHGWCAEALHGKYGATMELVTLRKKN 575
           PTVDDGHGWCAEALHGKYGATMELVTLRKK+
Sbjct: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKKS 571

BLAST of Chy6G111130 vs. ExPASy TrEMBL
Match: A0A6J1GXD4 (heat shock 70 kDa protein 8 OS=Cucurbita moschata OX=3662 GN=LOC111457701 PE=3 SV=1)

HSP 1 Score: 1047.0 bits (2706), Expect = 3.0e-302
Identity = 531/572 (92.83%), Postives = 554/572 (96.85%), Query Frame = 0

Query: 4   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 63
           MAEPIYTVASDSETTGEDR+ S FPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM
Sbjct: 1   MAEPIYTVASDSETTGEDRSASIFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60

Query: 64  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 123
           RSYVTFKDDTPSGGV NELS EYDMLSGAAIFNMKRLIGRVDTDPVVHA KSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVINELSQEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120

Query: 124 DIGVRPFVAALVNNVWRSTTPEEVLAIFIVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 183
           DIGVRPFVAALVNN+WRSTTPEEVLAIF+VELKMMAE+QLKRPIRNVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNIWRSTTPEEVLAIFLVELKMMAEHQLKRPIRNVVLTIPVSFSRFQL 180

Query: 184 TRVERACAMAGLQVRLMPEPTAVALLYA-QQQQQAVHDSMGSGSEKIALIFNMGAGYCDV 243
           TRVERACAMAGLQVRLMPEPTAVALLYA QQQQQAVH++MGSGSEKIALIFNMGAGYCDV
Sbjct: 181 TRVERACAMAGLQVRLMPEPTAVALLYAQQQQQQAVHENMGSGSEKIALIFNMGAGYCDV 240

Query: 244 AVSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQ 303
           AVSA GGGVSQI+ALAGSPIGGEDLLQNTMKYLLPN+ESIFSHRGIDEIRRMGLLRVATQ
Sbjct: 241 AVSATGGGVSQIKALAGSPIGGEDLLQNTMKYLLPNAESIFSHRGIDEIRRMGLLRVATQ 300

Query: 304 DAIHKLSLQSSVEINVSLGNGSKLCKVLSREDFEMVNSKVFEKCENLVKQCLHDARVGIE 363
           DAIHKLS QSSV+I+V+LGNGSKLCKVLSRE+FEMVNS+VFEKCENLV QCLHDARVGI+
Sbjct: 301 DAIHKLSFQSSVQIDVNLGNGSKLCKVLSREEFEMVNSEVFEKCENLVNQCLHDARVGID 360

Query: 364 DLSDVVVVGGCSYIPKIRHLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGS 423
           DLSDVVVVGGCS IPKIR  LME SKKKALYEG++PLEAAV GAALEGA+ASGI DPFGS
Sbjct: 361 DLSDVVVVGGCSNIPKIRSFLMEKSKKKALYEGMNPLEAAVCGAALEGAVASGIADPFGS 420

Query: 424 LDLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTIQDNQSEALIVVYEGEET 483
           LDLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTT++DNQSEALIVVYEGEET
Sbjct: 421 LDLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVKDNQSEALIVVYEGEET 480

Query: 484 RAEENHLLGYFKIIGIPPAPKGIPEITICMDIDSSNMLRVLAGATLPGAQHPATPYMEVK 543
           RAEENHLLGYFKIIGIPPAPKGIPEITICMDIDSSNMLRVLAGA LPGAQHPATP+MEVK
Sbjct: 481 RAEENHLLGYFKIIGIPPAPKGIPEITICMDIDSSNMLRVLAGARLPGAQHPATPFMEVK 540

Query: 544 MPTVDDGHGWCAEALHGKYGATMELVTLRKKN 575
           MPTVDDG GWCAEALHGKYGA M+LV+LRKK+
Sbjct: 541 MPTVDDGQGWCAEALHGKYGAAMDLVSLRKKS 572

BLAST of Chy6G111130 vs. ExPASy TrEMBL
Match: A0A6J1C992 (heat shock 70 kDa protein 8 OS=Momordica charantia OX=3673 GN=LOC111009482 PE=3 SV=1)

HSP 1 Score: 1045.4 bits (2702), Expect = 8.6e-302
Identity = 525/571 (91.94%), Postives = 552/571 (96.67%), Query Frame = 0

Query: 4   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 63
           MAEPIYTVASDSETTGE++TTSTFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM
Sbjct: 1   MAEPIYTVASDSETTGEEKTTSTFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60

Query: 64  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 123
           RSYVTFKDDTPSGGVS+EL HEYDMLSGAAIFNMKRLIGRVDTDPVVHA KSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSSELCHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120

Query: 124 DIGVRPFVAALVNNVWRSTTPEEVLAIFIVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 183
           DIGVRPFVAALVNN+WRSTTPEEVLAIF+VELKMMAEYQLKRPIRNVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNMWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 184 TRVERACAMAGLQVRLMPEPTAVALLYAQQQQQAVHDSMGSGSEKIALIFNMGAGYCDVA 243
           TRVERACAMAGLQVRLMPEPTAVALLYAQQQ QAVH++MGSGSEKIAL+FNMGAGYCDVA
Sbjct: 181 TRVERACAMAGLQVRLMPEPTAVALLYAQQQHQAVHENMGSGSEKIALVFNMGAGYCDVA 240

Query: 244 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 303
           +SA  GGVSQIRAL GSPIGGEDLLQNTMKYLLPNSESIFS+ GIDEIRRMGLLRVATQD
Sbjct: 241 ISATAGGVSQIRALTGSPIGGEDLLQNTMKYLLPNSESIFSNHGIDEIRRMGLLRVATQD 300

Query: 304 AIHKLSLQSSVEINVSLGNGSKLCKVLSREDFEMVNSKVFEKCENLVKQCLHDARVGIED 363
           AI KLS Q+S +I+V+LGNGSK+CKVLSRE+FEMVNSKVFEKCENLVKQCLHDARV IED
Sbjct: 301 AILKLSFQTSAQIDVNLGNGSKICKVLSREEFEMVNSKVFEKCENLVKQCLHDARVEIED 360

Query: 364 LSDVVVVGGCSYIPKIRHLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 423
           LSDVVVVGGCS IPKIR LLMEISKKKALYE IDPLEAAVSGAA+EGA+ASGIGDPFGSL
Sbjct: 361 LSDVVVVGGCSSIPKIRKLLMEISKKKALYEEIDPLEAAVSGAAIEGAVASGIGDPFGSL 420

Query: 424 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTIQDNQSEALIVVYEGEETR 483
           DLLSIQ TPLAIGIRADGN+FIPIIPKNTTMPARKELVFTT+QDNQSEALIVVYEGEE R
Sbjct: 421 DLLSIQVTPLAIGIRADGNNFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEEAR 480

Query: 484 AEENHLLGYFKIIGIPPAPKGIPEITICMDIDSSNMLRVLAGATLPGAQHPATPYMEVKM 543
           AEENHLLGYFKIIGIPPAPKG+PEITICMDIDSSN LRVLAGATLPGAQHPATP+MEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNTLRVLAGATLPGAQHPATPFMEVKM 540

Query: 544 PTVDDGHGWCAEALHGKYGATMELVTLRKKN 575
           PT+DDGH WCAEALHGK+G +M+LVTLRKK+
Sbjct: 541 PTIDDGHSWCAEALHGKHGVSMDLVTLRKKS 571

BLAST of Chy6G111130 vs. NCBI nr
Match: XP_004134140.1 (heat shock 70 kDa protein 8 [Cucumis sativus] >XP_011650982.1 heat shock 70 kDa protein 8 [Cucumis sativus] >KGN56991.1 hypothetical protein Csa_011014 [Cucumis sativus])

HSP 1 Score: 1113 bits (2880), Expect = 0.0
Identity = 567/571 (99.30%), Postives = 570/571 (99.82%), Query Frame = 0

Query: 4   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 63
           MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM
Sbjct: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60

Query: 64  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 123
           RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120

Query: 124 DIGVRPFVAALVNNVWRSTTPEEVLAIFIVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 183
           DIGVRPFVAALVNNVWRSTTPEEVLAIFIVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFIVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 184 TRVERACAMAGLQVRLMPEPTAVALLYAQQQQQAVHDSMGSGSEKIALIFNMGAGYCDVA 243
           TRVERACAMAGLQVRLMPEPTAVALLYAQQQQQAVHDSMGSGSEKIALIFNMGAGYCDVA
Sbjct: 181 TRVERACAMAGLQVRLMPEPTAVALLYAQQQQQAVHDSMGSGSEKIALIFNMGAGYCDVA 240

Query: 244 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 303
           VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD
Sbjct: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300

Query: 304 AIHKLSLQSSVEINVSLGNGSKLCKVLSREDFEMVNSKVFEKCENLVKQCLHDARVGIED 363
           AIHKLS QSSVEINVSLGNGSKLCKVLSREDFEMVNSKVFEKCENLVKQCLHDAR+GIED
Sbjct: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREDFEMVNSKVFEKCENLVKQCLHDARLGIED 360

Query: 364 LSDVVVVGGCSYIPKIRHLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 423
           LSDVVVVGGCSYIPKIRHLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL
Sbjct: 361 LSDVVVVGGCSYIPKIRHLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420

Query: 424 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTIQDNQSEALIVVYEGEETR 483
           DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTIQDNQSEALIVVYEGEETR
Sbjct: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTIQDNQSEALIVVYEGEETR 480

Query: 484 AEENHLLGYFKIIGIPPAPKGIPEITICMDIDSSNMLRVLAGATLPGAQHPATPYMEVKM 543
           AEENHLLGYFKIIGIPPAPKG+PEITICMDIDSSNMLRVLAGATLPGAQHPATPYMEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGAQHPATPYMEVKM 540

Query: 544 PTVDDGHGWCAEALHGKYGATMELVTLRKKN 574
           PTVDDGHGWCAEALHGKYGATMELVTLRKK+
Sbjct: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKKS 571

BLAST of Chy6G111130 vs. NCBI nr
Match: KAA0049367.1 (heat shock 70 kDa protein 8 [Cucumis melo var. makuwa] >TYK17192.1 heat shock 70 kDa protein 8 [Cucumis melo var. makuwa])

HSP 1 Score: 1098 bits (2841), Expect = 0.0
Identity = 559/571 (97.90%), Postives = 565/571 (98.95%), Query Frame = 0

Query: 4   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 63
           MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGS VELLKNTR+QKIM
Sbjct: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIM 60

Query: 64  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 123
           RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120

Query: 124 DIGVRPFVAALVNNVWRSTTPEEVLAIFIVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 183
           DIGVRPFVAALVNNVWRSTTPEEVLAIF+VELKMMAEYQLKRPIRNVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 184 TRVERACAMAGLQVRLMPEPTAVALLYAQQQQQAVHDSMGSGSEKIALIFNMGAGYCDVA 243
           TRVERACAMAG QVRLMPEPTAVALLYAQQQQQA HDSMGSGSEKIALIFNMGAGYCDVA
Sbjct: 181 TRVERACAMAGFQVRLMPEPTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDVA 240

Query: 244 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 303
           VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD
Sbjct: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300

Query: 304 AIHKLSLQSSVEINVSLGNGSKLCKVLSREDFEMVNSKVFEKCENLVKQCLHDARVGIED 363
           AIHKLS QSSVEINVSLGNGSKLCKVLSRE+FEMVNSKVFEKCENLVKQCLHDARVGIED
Sbjct: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIED 360

Query: 364 LSDVVVVGGCSYIPKIRHLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 423
           LSDVVVVGGCSYIPKIR LLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL
Sbjct: 361 LSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420

Query: 424 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTIQDNQSEALIVVYEGEETR 483
           DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTT+QDNQSEALIVVYEGEETR
Sbjct: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETR 480

Query: 484 AEENHLLGYFKIIGIPPAPKGIPEITICMDIDSSNMLRVLAGATLPGAQHPATPYMEVKM 543
           AEENHLLGYFKIIGIPPAPKG+PEITICMDIDSSNMLRVLAGA LPGAQHPATPYMEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYMEVKM 540

Query: 544 PTVDDGHGWCAEALHGKYGATMELVTLRKKN 574
           PTVDDGHGWCAEALHGKYGATMELVTLRKK+
Sbjct: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKKS 571

BLAST of Chy6G111130 vs. NCBI nr
Match: XP_008438682.1 (PREDICTED: heat shock 70 kDa protein 8 [Cucumis melo])

HSP 1 Score: 1097 bits (2837), Expect = 0.0
Identity = 558/571 (97.72%), Postives = 564/571 (98.77%), Query Frame = 0

Query: 4   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 63
           MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGS VELLKNTR+QKIM
Sbjct: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIM 60

Query: 64  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 123
           RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120

Query: 124 DIGVRPFVAALVNNVWRSTTPEEVLAIFIVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 183
           DIGVRPFVAALVNNVWRSTTPEEVLAIF+VELKMMAEYQLKRPIRNVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 184 TRVERACAMAGLQVRLMPEPTAVALLYAQQQQQAVHDSMGSGSEKIALIFNMGAGYCDVA 243
           TRVERACAMAG QVRLMPEPTAVALLYAQQQQQA HDSMGSGSEKIALIFNMGAGYCD A
Sbjct: 181 TRVERACAMAGFQVRLMPEPTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDAA 240

Query: 244 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 303
           VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD
Sbjct: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300

Query: 304 AIHKLSLQSSVEINVSLGNGSKLCKVLSREDFEMVNSKVFEKCENLVKQCLHDARVGIED 363
           AIHKLS QSSVEINVSLGNGSKLCKVLSRE+FEMVNSKVFEKCENLVKQCLHDARVGIED
Sbjct: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIED 360

Query: 364 LSDVVVVGGCSYIPKIRHLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 423
           LSDVVVVGGCSYIPKIR LLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL
Sbjct: 361 LSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420

Query: 424 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTIQDNQSEALIVVYEGEETR 483
           DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTT+QDNQSEALIVVYEGEETR
Sbjct: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETR 480

Query: 484 AEENHLLGYFKIIGIPPAPKGIPEITICMDIDSSNMLRVLAGATLPGAQHPATPYMEVKM 543
           AEENHLLGYFKIIGIPPAPKG+PEITICMDIDSSNMLRVLAGA LPGAQHPATPYMEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYMEVKM 540

Query: 544 PTVDDGHGWCAEALHGKYGATMELVTLRKKN 574
           PTVDDGHGWCAEALHGKYGATMELVTLRKK+
Sbjct: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKKS 571

BLAST of Chy6G111130 vs. NCBI nr
Match: XP_038897373.1 (heat shock 70 kDa protein 8 [Benincasa hispida])

HSP 1 Score: 1075 bits (2781), Expect = 0.0
Identity = 546/571 (95.62%), Postives = 563/571 (98.60%), Query Frame = 0

Query: 4   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 63
           MAEPIYTVASDSETTGED+TTSTFP+IAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM
Sbjct: 1   MAEPIYTVASDSETTGEDKTTSTFPDIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60

Query: 64  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 123
           RSYVTFKDDTPSGGVSNELSHE+DMLSGAAIFNMKRLIGRVDTDPVVHA KSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSNELSHEFDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120

Query: 124 DIGVRPFVAALVNNVWRSTTPEEVLAIFIVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 183
           DIGVRPFVAALVNNVWRSTTPEEVLAIFIVELKMMAEYQLKR I+NVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFIVELKMMAEYQLKRSIKNVVLTIPVSFSRFQL 180

Query: 184 TRVERACAMAGLQVRLMPEPTAVALLYAQQQQQAVHDSMGSGSEKIALIFNMGAGYCDVA 243
           TRVERACAMAGLQVRLMPEPTAVALLYAQQQQQAVH+SMGSGSEKIALIFNMGAGYCDVA
Sbjct: 181 TRVERACAMAGLQVRLMPEPTAVALLYAQQQQQAVHESMGSGSEKIALIFNMGAGYCDVA 240

Query: 244 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 303
           VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD
Sbjct: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300

Query: 304 AIHKLSLQSSVEINVSLGNGSKLCKVLSREDFEMVNSKVFEKCENLVKQCLHDARVGIED 363
           AIHKLS QSSV+I+V+LGNGSKLCKVLSRE+FEMVNSKVFEKCENLVKQCLHDARV IED
Sbjct: 301 AIHKLSFQSSVQIDVNLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVRIED 360

Query: 364 LSDVVVVGGCSYIPKIRHLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 423
           LSDVVVVGGCSYIPKIR+LLMEI K KALYEG+DPLEAAVSGAALEGAIASGIGDPFGSL
Sbjct: 361 LSDVVVVGGCSYIPKIRNLLMEICKNKALYEGMDPLEAAVSGAALEGAIASGIGDPFGSL 420

Query: 424 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTIQDNQSEALIVVYEGEETR 483
           DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTT++DNQSEALIVVYEGEETR
Sbjct: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVRDNQSEALIVVYEGEETR 480

Query: 484 AEENHLLGYFKIIGIPPAPKGIPEITICMDIDSSNMLRVLAGATLPGAQHPATPYMEVKM 543
           AEENHLLGY KIIGIPPAPKG+PEITICMDIDSSNMLRVLAGATLPG+QHPATPYMEVKM
Sbjct: 481 AEENHLLGYVKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGSQHPATPYMEVKM 540

Query: 544 PTVDDGHGWCAEALHGKYGATMELVTLRKKN 574
           PTVDDGHGWCAEALHGKYG TMELV+LRKK+
Sbjct: 541 PTVDDGHGWCAEALHGKYGPTMELVSLRKKS 571

BLAST of Chy6G111130 vs. NCBI nr
Match: XP_023527608.1 (heat shock 70 kDa protein 8 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1048 bits (2709), Expect = 0.0
Identity = 535/575 (93.04%), Postives = 556/575 (96.70%), Query Frame = 0

Query: 1   MQIMAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQ 60
           MQIMAEPIYTVASDSETTGEDR+ S FPEI VGIDIGTSQCSIAVWNGSQVELLKNTRNQ
Sbjct: 1   MQIMAEPIYTVASDSETTGEDRSASIFPEIDVGIDIGTSQCSIAVWNGSQVELLKNTRNQ 60

Query: 61  KIMRSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLV 120
           KIMRSYVTFKDDTPSGGV NELS EYDMLSGAAIFNMKRLIGRVDTDPVVHA KSLPFLV
Sbjct: 61  KIMRSYVTFKDDTPSGGVINELSQEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLV 120

Query: 121 QTLDIGVRPFVAALVNNVWRSTTPEEVLAIFIVELKMMAEYQLKRPIRNVVLTIPVSFSR 180
           QTLDIGVRPFVAALVNNVWRSTTPEEVLAIF+VELKMMAE+QLKRPIRNVVLTIPVSFSR
Sbjct: 121 QTLDIGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEHQLKRPIRNVVLTIPVSFSR 180

Query: 181 FQLTRVERACAMAGLQVRLMPEPTAVALLYAQQQQQ-AVHDSMGSGSEKIALIFNMGAGY 240
           FQLTRVERACAMAGLQVRLMPEPTAVALLYAQQQQQ A H++MGSGSEKIALIFNMGAGY
Sbjct: 181 FQLTRVERACAMAGLQVRLMPEPTAVALLYAQQQQQQAAHENMGSGSEKIALIFNMGAGY 240

Query: 241 CDVAVSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRV 300
           CDVAVSA GGGVSQI+ALAGSPIGGEDLLQNTMKYLLPN+ESIFSHRGIDEIRRMGLLRV
Sbjct: 241 CDVAVSATGGGVSQIKALAGSPIGGEDLLQNTMKYLLPNAESIFSHRGIDEIRRMGLLRV 300

Query: 301 ATQDAIHKLSLQSSVEINVSLGNGSKLCKVLSREDFEMVNSKVFEKCENLVKQCLHDARV 360
           ATQDAIHKLS QSSV+I+V+LGNGSKLCKVLSRE+FEMVNS+VFEKCENLV  CLHDARV
Sbjct: 301 ATQDAIHKLSFQSSVQIDVNLGNGSKLCKVLSREEFEMVNSEVFEKCENLVNLCLHDARV 360

Query: 361 GIEDLSDVVVVGGCSYIPKIRHLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDP 420
           GIEDLSDVVVVGGCS IPKIR+LLM  SKKKALYEG++PLEAAVSGAALEGA+ASGI DP
Sbjct: 361 GIEDLSDVVVVGGCSNIPKIRNLLMGKSKKKALYEGMNPLEAAVSGAALEGAVASGIADP 420

Query: 421 FGSLDLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTIQDNQSEALIVVYEG 480
           FGSLDLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTT++DNQSEALIVVYEG
Sbjct: 421 FGSLDLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVKDNQSEALIVVYEG 480

Query: 481 EETRAEENHLLGYFKIIGIPPAPKGIPEITICMDIDSSNMLRVLAGATLPGAQHPATPYM 540
           EETRAEENHLLGYFKIIGIPPAPKGIPEITICMDIDSSNMLRVLAGA LPG QHPATP+M
Sbjct: 481 EETRAEENHLLGYFKIIGIPPAPKGIPEITICMDIDSSNMLRVLAGARLPGVQHPATPFM 540

Query: 541 EVKMPTVDDGHGWCAEALHGKYGATMELVTLRKKN 574
           EVKMPTVDDGHGWCAEALHGKYGA M+LVTLRKK+
Sbjct: 541 EVKMPTVDDGHGWCAEALHGKYGAAMDLVTLRKKS 575

BLAST of Chy6G111130 vs. TAIR 10
Match: AT2G32120.1 (heat-shock protein 70T-2 )

HSP 1 Score: 835.9 bits (2158), Expect = 2.0e-242
Identity = 404/573 (70.51%), Postives = 491/573 (85.69%), Query Frame = 0

Query: 4   MAEPIYTVASDSETTGEDRTTS--TFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQK 63
           MAE  YTVASDSE TGE++++S  + PEIA+GIDIGTSQCSIAVWNGSQV +L+NTRNQK
Sbjct: 1   MAEAAYTVASDSENTGEEKSSSSPSLPEIALGIDIGTSQCSIAVWNGSQVHILRNTRNQK 60

Query: 64  IMRSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQ 123
           +++S+VTFKD+ P+GGVSN+L+HE +ML+GAAIFNMKRL+GRVDTDPVVHA K+LPFLVQ
Sbjct: 61  LIKSFVTFKDEVPAGGVSNQLAHEQEMLTGAAIFNMKRLVGRVDTDPVVHASKNLPFLVQ 120

Query: 124 TLDIGVRPFVAALVNNVWRSTTPEEVLAIFIVELKMMAEYQLKRPIRNVVLTIPVSFSRF 183
           TLDIGVRPF+AALVNN WRSTTPEEVLAIF+VEL++MAE QLKRP+RNVVLT+PVSFSRF
Sbjct: 121 TLDIGVRPFIAALVNNAWRSTTPEEVLAIFLVELRLMAEAQLKRPVRNVVLTVPVSFSRF 180

Query: 184 QLTRVERACAMAGLQV-RLMPEPTAVALLYAQQQQQAVHDSMGSGSEKIALIFNMGAGYC 243
           QLTR ERACAMAGL V RLMPEPTA+ALLYAQQQQ   HD+MGSGSE++A+IFNMGAGYC
Sbjct: 181 QLTRFERACAMAGLHVLRLMPEPTAIALLYAQQQQMTTHDNMGSGSERLAVIFNMGAGYC 240

Query: 244 DVAVSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVA 303
           DVAV+A  GGVSQI+ALAGSPIGGED+LQNT++++ P +E              GLLRVA
Sbjct: 241 DVAVTATAGGVSQIKALAGSPIGGEDILQNTIRHIAPPNE-----------EASGLLRVA 300

Query: 304 TQDAIHKLSLQSSVEINVSLGNGSKLCKVLSREDFEMVNSKVFEKCENLVKQCLHDARVG 363
            QDAIH+L+ Q +V+I V LGNG+K+ KVL R +FE VN KVFE+CE LV QCL DARV 
Sbjct: 301 AQDAIHRLTDQENVQIEVDLGNGNKISKVLDRLEFEEVNQKVFEECERLVVQCLRDARVN 360

Query: 364 IEDLSDVVVVGGCSYIPKIRHLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPF 423
             D+ D+++VGGCSYIPK+R ++  + KK  +Y+G++PLEAAV GAALEGA+ SGI DPF
Sbjct: 361 GGDIDDLIMVGGCSYIPKVRTIIKNVCKKDEIYKGVNPLEAAVRGAALEGAVTSGIHDPF 420

Query: 424 GSLDLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTIQDNQSEALIVVYEGE 483
           GSLDLL+IQATPLA+G+RA+GN FIP+IP+NT +PARK+L FTT+QDNQ EALI++YEGE
Sbjct: 421 GSLDLLTIQATPLAVGVRANGNKFIPVIPRNTMVPARKDLFFTTVQDNQKEALIIIYEGE 480

Query: 484 ETRAEENHLLGYFKIIGIPPAPKGIPEITICMDIDSSNMLRVLAGATLPGAQHPATPYME 543
               EENHLLGYFK++GIPPAPKG+PEI +CMDID+SN LRV A   +PG+  P  P +E
Sbjct: 481 GETVEENHLLGYFKLVGIPPAPKGVPEINVCMDIDASNALRVFAAVLMPGSSSPVVPVIE 540

Query: 544 VKMPTVDDGHGWCAEALHGKYGATMELVTLRKK 574
           V+MPTVDDGHGWCA+AL+ KYGAT++L+TL++K
Sbjct: 541 VRMPTVDDGHGWCAQALNVKYGATLDLITLQRK 562

BLAST of Chy6G111130 vs. TAIR 10
Match: AT2G32120.2 (heat-shock protein 70T-2 )

HSP 1 Score: 835.9 bits (2158), Expect = 2.0e-242
Identity = 404/573 (70.51%), Postives = 491/573 (85.69%), Query Frame = 0

Query: 4   MAEPIYTVASDSETTGEDRTTS--TFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQK 63
           MAE  YTVASDSE TGE++++S  + PEIA+GIDIGTSQCSIAVWNGSQV +L+NTRNQK
Sbjct: 1   MAEAAYTVASDSENTGEEKSSSSPSLPEIALGIDIGTSQCSIAVWNGSQVHILRNTRNQK 60

Query: 64  IMRSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQ 123
           +++S+VTFKD+ P+GGVSN+L+HE +ML+GAAIFNMKRL+GRVDTDPVVHA K+LPFLVQ
Sbjct: 61  LIKSFVTFKDEVPAGGVSNQLAHEQEMLTGAAIFNMKRLVGRVDTDPVVHASKNLPFLVQ 120

Query: 124 TLDIGVRPFVAALVNNVWRSTTPEEVLAIFIVELKMMAEYQLKRPIRNVVLTIPVSFSRF 183
           TLDIGVRPF+AALVNN WRSTTPEEVLAIF+VEL++MAE QLKRP+RNVVLT+PVSFSRF
Sbjct: 121 TLDIGVRPFIAALVNNAWRSTTPEEVLAIFLVELRLMAEAQLKRPVRNVVLTVPVSFSRF 180

Query: 184 QLTRVERACAMAGLQV-RLMPEPTAVALLYAQQQQQAVHDSMGSGSEKIALIFNMGAGYC 243
           QLTR ERACAMAGL V RLMPEPTA+ALLYAQQQQ   HD+MGSGSE++A+IFNMGAGYC
Sbjct: 181 QLTRFERACAMAGLHVLRLMPEPTAIALLYAQQQQMTTHDNMGSGSERLAVIFNMGAGYC 240

Query: 244 DVAVSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVA 303
           DVAV+A  GGVSQI+ALAGSPIGGED+LQNT++++ P +E              GLLRVA
Sbjct: 241 DVAVTATAGGVSQIKALAGSPIGGEDILQNTIRHIAPPNE-----------EASGLLRVA 300

Query: 304 TQDAIHKLSLQSSVEINVSLGNGSKLCKVLSREDFEMVNSKVFEKCENLVKQCLHDARVG 363
            QDAIH+L+ Q +V+I V LGNG+K+ KVL R +FE VN KVFE+CE LV QCL DARV 
Sbjct: 301 AQDAIHRLTDQENVQIEVDLGNGNKISKVLDRLEFEEVNQKVFEECERLVVQCLRDARVN 360

Query: 364 IEDLSDVVVVGGCSYIPKIRHLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPF 423
             D+ D+++VGGCSYIPK+R ++  + KK  +Y+G++PLEAAV GAALEGA+ SGI DPF
Sbjct: 361 GGDIDDLIMVGGCSYIPKVRTIIKNVCKKDEIYKGVNPLEAAVRGAALEGAVTSGIHDPF 420

Query: 424 GSLDLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTIQDNQSEALIVVYEGE 483
           GSLDLL+IQATPLA+G+RA+GN FIP+IP+NT +PARK+L FTT+QDNQ EALI++YEGE
Sbjct: 421 GSLDLLTIQATPLAVGVRANGNKFIPVIPRNTMVPARKDLFFTTVQDNQKEALIIIYEGE 480

Query: 484 ETRAEENHLLGYFKIIGIPPAPKGIPEITICMDIDSSNMLRVLAGATLPGAQHPATPYME 543
               EENHLLGYFK++GIPPAPKG+PEI +CMDID+SN LRV A   +PG+  P  P +E
Sbjct: 481 GETVEENHLLGYFKLVGIPPAPKGVPEINVCMDIDASNALRVFAAVLMPGSSSPVVPVIE 540

Query: 544 VKMPTVDDGHGWCAEALHGKYGATMELVTLRKK 574
           V+MPTVDDGHGWCA+AL+ KYGAT++L+TL++K
Sbjct: 541 VRMPTVDDGHGWCAQALNVKYGATLDLITLQRK 562

BLAST of Chy6G111130 vs. TAIR 10
Match: AT3G12580.1 (heat shock protein 70 )

HSP 1 Score: 290.0 bits (741), Expect = 4.1e-78
Identity = 180/511 (35.23%), Postives = 287/511 (56.16%), Query Frame = 0

Query: 31  AVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIMRSYVTFKDDTPSGGVSNELSHEYDMLS 90
           A+GID+GT+   + VW   +VE++ N +  +   SYV F D      + +   ++  M  
Sbjct: 9   AIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERL--IGDAAKNQVAMNP 68

Query: 91  GAAIFNMKRLIGRVDTDPVVHACKS-LPFLVQTLDIGVRPFVAALVNNVWRSTTPEEVLA 150
              +F+ KRLIGR  +DP V A KS  PF V +   G +P +        +  + EE+ +
Sbjct: 69  TNTVFDAKRLIGRRYSDPSVQADKSHWPFKVVS-GPGEKPMIVVNHKGEEKQFSAEEISS 128

Query: 151 IFIVELKMMAEYQLKRPIRNVVLTIPVSFSRFQLTRVERACAMAGLQV-RLMPEPTAVAL 210
           + +++++ +AE  L  P++N V+T+P  F+  Q    + A  ++GL V R++ EPTA A+
Sbjct: 129 MVLIKMREIAEAFLGSPVKNAVVTVPAYFNDSQRQATKDAGVISGLNVMRIINEPTAAAI 188

Query: 211 LYAQQQQQAVHDSMGSGSEKIALIFNMGAGYCDVAVSAIGGGVSQIRALAG-SPIGGEDL 270
            Y   ++ +      S  EK  LIF++G G  DV++  I  G+ +++A AG + +GGED 
Sbjct: 189 AYGLDKKAS------SVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDF 248

Query: 271 LQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQDAIHKLS--LQSSVEINVSLGNGSK 330
               + + +   +           R +  LR A + A   LS   Q+++EI+ SL  G  
Sbjct: 249 DNRMVNHFVQEFKRKNKKDITGNPRALRRLRTACERAKRTLSSTAQTTIEID-SLFEGID 308

Query: 331 LCKVLSREDFEMVNSKVFEKCENLVKQCLHDARVGIEDLSDVVVVGGCSYIPKIRHLLME 390
               ++R  FE +N  +F KC   V++CL DA++    + DVV+VGG + IPK++ LL +
Sbjct: 309 FYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQD 368

Query: 391 ISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSLDLLSIQATPLAIGIRADGNSFI 450
               K L + I+P EA   GAA++ AI SG G+     DLL +  TPL++G+   G    
Sbjct: 369 FFNGKELCKSINPDEAVAYGAAVQAAILSGEGNE-KVQDLLLLDVTPLSLGLETAGGVMT 428

Query: 451 PIIPKNTTMPARKELVFTTIQDNQSEALIVVYEGEETRAEENHLLGYFKIIGIPPAPKGI 510
            +IP+NTT+P +KE +F+T  DNQ   LI VYEGE  R ++N+LLG F++ GIPPAP+G+
Sbjct: 429 VLIPRNTTIPTKKEQIFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPAPRGV 488

Query: 511 PEITICMDIDSSNMLRVLAGATLPGAQHPAT 537
           P+IT+C DID++ +L V A     G ++  T
Sbjct: 489 PQITVCFDIDANGILNVSAEDKTTGQKNKIT 508

BLAST of Chy6G111130 vs. TAIR 10
Match: AT1G16030.1 (heat shock protein 70B )

HSP 1 Score: 287.0 bits (733), Expect = 3.4e-77
Identity = 182/517 (35.20%), Postives = 284/517 (54.93%), Query Frame = 0

Query: 25  STFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIMRSYVTFKDDTPSGGVSNELSH 84
           +T  E A+GID+GT+   + VW   +VE++ N +  +   SYV F D      + +   +
Sbjct: 2   ATKSEKAIGIDLGTTYSCVGVWMNDRVEIIPNDQGNRTTPSYVAFTDTERL--IGDAAKN 61

Query: 85  EYDMLSGAAIFNMKRLIGRVDTDPVVHA-CKSLPFLVQTLDIGVRPFVAALVNNVWRSTT 144
           +  +     +F+ KRLIGR  +DP V +     PF V +   G +P +     N  +  +
Sbjct: 62  QVALNPQNTVFDAKRLIGRKFSDPSVQSDILHWPFKVVS-GPGEKPMIVVSYKNEEKQFS 121

Query: 145 PEEVLAIFIVELKMMAEYQLKRPIRNVVLTIPVSFSRFQLTRVERACAMAGLQV-RLMPE 204
           PEE+ ++ +V++K +AE  L R ++N V+T+P  F+  Q    + A A++GL V R++ E
Sbjct: 122 PEEISSMVLVKMKEVAEAFLGRTVKNAVVTVPAYFNDSQRQATKDAGAISGLNVLRIINE 181

Query: 205 PTAVALLYAQQQQQAVHDSMGSGSEKIALIFNMGAGYCDVAVSAIGGGVSQIRALAG-SP 264
           PTA A+ Y   ++           EK  LIF++G G  DV++  I  GV +++A AG + 
Sbjct: 182 PTAAAIAYGLDKKGT------KAGEKNVLIFDLGGGTFDVSLLTIEEGVFEVKATAGDTH 241

Query: 265 IGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQDAIHKLS--LQSSVEINVS 324
           +GGED     + + +               R +  LR A + A   LS   Q+++EI+ S
Sbjct: 242 LGGEDFDNRLVNHFVAEFRRKHKKDIAGNARALRRLRTACERAKRTLSSTAQTTIEID-S 301

Query: 325 LGNGSKLCKVLSREDFEMVNSKVFEKCENLVKQCLHDARVGIEDLSDVVVVGGCSYIPKI 384
           L  G      +SR  FE +N  +F KC + V++ L DA++    + DVV+VGG + IPKI
Sbjct: 302 LHEGIDFYATISRARFEEMNMDLFRKCMDPVEKVLKDAKLDKSSVHDVVLVGGSTRIPKI 361

Query: 385 RHLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSLDLLSIQATPLAIGIRA 444
           + LL +    K L + I+P EA   GAA++ AI +G G      DLL +   PL++G+  
Sbjct: 362 QQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILTGEGSE-KVQDLLLLDVAPLSLGLET 421

Query: 445 DGNSFIPIIPKNTTMPARKELVFTTIQDNQSEALIVVYEGEETRAEENHLLGYFKIIGIP 504
            G     +IP+NTT+P +KE VF+T  DNQ   LI VYEGE  R  +N+LLG F++ GIP
Sbjct: 422 AGGVMTVLIPRNTTVPCKKEQVFSTYADNQPGVLIQVYEGERARTRDNNLLGTFELKGIP 481

Query: 505 PAPKGIPEITICMDIDSSNMLRVLAGATLPGAQHPAT 537
           PAP+G+P+I +C DID++ +L V A     G ++  T
Sbjct: 482 PAPRGVPQINVCFDIDANGILNVSAEDKTAGVKNQIT 507

BLAST of Chy6G111130 vs. TAIR 10
Match: AT5G02500.1 (heat shock cognate protein 70-1 )

HSP 1 Score: 283.1 bits (723), Expect = 5.0e-76
Identity = 178/511 (34.83%), Postives = 282/511 (55.19%), Query Frame = 0

Query: 31  AVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIMRSYVTFKDDTPSGGVSNELSHEYDMLS 90
           A+GID+GT+   + VW   +VE++ N +  +   SYV F D      + +   ++  M  
Sbjct: 9   AIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERL--IGDAAKNQVAMNP 68

Query: 91  GAAIFNMKRLIGRVDTDPVVHA-CKSLPFLVQTLDIGVRPFVAALVNNVWRSTTPEEVLA 150
              +F+ KRLIGR  +D  V +  K  PF +Q      +P +        +    EE+ +
Sbjct: 69  VNTVFDAKRLIGRRFSDSSVQSDMKLWPFKIQA-GPADKPMIYVEYKGEEKEFAAEEISS 128

Query: 151 IFIVELKMMAEYQLKRPIRNVVLTIPVSFSRFQLTRVERACAMAGLQV-RLMPEPTAVAL 210
           + +++++ +AE  L   I+N V+T+P  F+  Q    + A  +AGL V R++ EPTA A+
Sbjct: 129 MVLIKMREIAEAYLGVTIKNAVVTVPAYFNDSQRQATKDAGVIAGLNVMRIINEPTAAAI 188

Query: 211 LYAQQQQQAVHDSMGSGSEKIALIFNMGAGYCDVAVSAIGGGVSQIRALAG-SPIGGEDL 270
            Y   ++        S  EK  LIF++G G  DV++  I  G+ +++A AG + +GGED 
Sbjct: 189 AYGLDKKAT------SVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDF 248

Query: 271 LQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQDAIHKLS--LQSSVEINVSLGNGSK 330
               + + +   +           R +  LR + + A   LS   Q+++EI+ SL  G  
Sbjct: 249 DNRMVNHFVQEFKRKSKKDITGNPRALRRLRTSCERAKRTLSSTAQTTIEID-SLYEGID 308

Query: 331 LCKVLSREDFEMVNSKVFEKCENLVKQCLHDARVGIEDLSDVVVVGGCSYIPKIRHLLME 390
               ++R  FE +N  +F KC   V++CL DA++    + DVV+VGG + IPK++ LL +
Sbjct: 309 FYSTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQQLLQD 368

Query: 391 ISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSLDLLSIQATPLAIGIRADGNSFI 450
               K L + I+P EA   GAA++GAI SG G+     DLL +  TPL++G+   G    
Sbjct: 369 FFNGKELCKSINPDEAVAYGAAVQGAILSGEGNE-KVQDLLLLDVTPLSLGLETAGGVMT 428

Query: 451 PIIPKNTTMPARKELVFTTIQDNQSEALIVVYEGEETRAEENHLLGYFKIIGIPPAPKGI 510
            +IP+NTT+P +KE VF+T  DNQ   LI VYEGE  R ++N+LLG F++ GIPPAP+G+
Sbjct: 429 TLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPAPRGV 488

Query: 511 PEITICMDIDSSNMLRVLAGATLPGAQHPAT 537
           P+IT+C DID++ +L V A     G ++  T
Sbjct: 489 PQITVCFDIDANGILNVSAEDKTTGQKNKIT 508

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SKY82.8e-24170.51Heat shock 70 kDa protein 8 OS=Arabidopsis thaliana OX=3702 GN=HSP70-8 PE=1 SV=1[more]
Q9LHA85.7e-7735.23Heat shock 70 kDa protein 4 OS=Arabidopsis thaliana OX=3702 GN=HSP70-4 PE=1 SV=1[more]
Q9S9N14.8e-7635.20Heat shock 70 kDa protein 5 OS=Arabidopsis thaliana OX=3702 GN=HSP70-5 PE=2 SV=1[more]
P091896.3e-7635.53Heat shock cognate 70 kDa protein OS=Petunia hybrida OX=4102 GN=HSP70 PE=2 SV=1[more]
P229537.0e-7534.83Heat shock 70 kDa protein 1 OS=Arabidopsis thaliana OX=3702 GN=HSP70-1 PE=1 SV=3[more]
Match NameE-valueIdentityDescription
A0A0A0LAC60.0e+0099.30Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G147740 PE=3 SV=1[more]
A0A5D3D0520.0e+0097.90Heat shock 70 kDa protein 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3AXP00.0e+0097.72heat shock 70 kDa protein 8 OS=Cucumis melo OX=3656 GN=LOC103483715 PE=3 SV=1[more]
A0A6J1GXD43.0e-30292.83heat shock 70 kDa protein 8 OS=Cucurbita moschata OX=3662 GN=LOC111457701 PE=3 S... [more]
A0A6J1C9928.6e-30291.94heat shock 70 kDa protein 8 OS=Momordica charantia OX=3673 GN=LOC111009482 PE=3 ... [more]
Match NameE-valueIdentityDescription
XP_004134140.10.099.30heat shock 70 kDa protein 8 [Cucumis sativus] >XP_011650982.1 heat shock 70 kDa ... [more]
KAA0049367.10.097.90heat shock 70 kDa protein 8 [Cucumis melo var. makuwa] >TYK17192.1 heat shock 70... [more]
XP_008438682.10.097.72PREDICTED: heat shock 70 kDa protein 8 [Cucumis melo][more]
XP_038897373.10.095.62heat shock 70 kDa protein 8 [Benincasa hispida][more]
XP_023527608.10.093.04heat shock 70 kDa protein 8 isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT2G32120.12.0e-24270.51heat-shock protein 70T-2 [more]
AT2G32120.22.0e-24270.51heat-shock protein 70T-2 [more]
AT3G12580.14.1e-7835.23heat shock protein 70 [more]
AT1G16030.13.4e-7735.20heat shock protein 70B [more]
AT5G02500.15.0e-7634.83heat shock cognate protein 70-1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00301HEATSHOCK70coord: 30..43
score: 41.3
coord: 238..248
score: 36.71
coord: 424..443
score: 36.79
coord: 505..521
score: 31.37
coord: 170..190
score: 28.47
coord: 364..380
score: 43.76
coord: 58..70
score: 27.83
coord: 396..416
score: 43.48
NoneNo IPR availableGENE3D3.90.640.10Actin; Chain A, domain 4coord: 264..345
e-value: 1.7E-79
score: 269.2
NoneNo IPR availableGENE3D3.30.30.30coord: 95..150
e-value: 1.7E-79
score: 269.2
NoneNo IPR availableGENE3D3.30.420.40coord: 32..411
e-value: 1.7E-79
score: 269.2
NoneNo IPR availableGENE3D3.30.420.40coord: 215..393
e-value: 1.7E-79
score: 269.2
NoneNo IPR availablePANTHERPTHR19375:SF101HEAT SHOCK 70 KDA PROTEIN 8coord: 7..573
NoneNo IPR availableCDDcd10170HSP70_NBDcoord: 32..411
e-value: 1.07225E-80
score: 256.001
IPR013126Heat shock protein 70 familyPFAMPF00012HSP70coord: 31..528
e-value: 2.1E-88
score: 297.1
IPR013126Heat shock protein 70 familyPANTHERPTHR19375HEAT SHOCK PROTEIN 70KDAcoord: 7..573
IPR029047Heat shock protein 70kD, peptide-binding domain superfamilyGENE3D2.60.34.10Substrate Binding Domain Of DNAk; Chain A, domain 1coord: 422..534
e-value: 2.8E-33
score: 117.0
IPR029047Heat shock protein 70kD, peptide-binding domain superfamilySUPERFAMILY100920Heat shock protein 70kD (HSP70), peptide-binding domaincoord: 426..525
IPR043129ATPase, nucleotide binding domainSUPERFAMILY53067Actin-like ATPase domaincoord: 30..212
IPR043129ATPase, nucleotide binding domainSUPERFAMILY53067Actin-like ATPase domaincoord: 226..412

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy6G111130.1Chy6G111130.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034620 cellular response to unfolded protein
biological_process GO:0051085 chaperone cofactor-dependent protein refolding
biological_process GO:0042026 protein refolding
biological_process GO:0009408 response to heat
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0031072 heat shock protein binding
molecular_function GO:0051787 misfolded protein binding
molecular_function GO:0044183 protein folding chaperone
molecular_function GO:0051082 unfolded protein binding