Chy6G110300 (gene) Cucumber (hystrix) v1

Overview


Name	Chy6G110300
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Protease inhibitor protein
Location	chrH06: 3406063 .. 3406469 (+)
RNA-Seq Expression	Chy6G110300
Synteny	Chy6G110300

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGAAGAATCTGCTCCAAGTAAGTTCATCCTATCAAAATGATCTTTTTTCAGAAACTAGTTTCGATTGAACGGTTTGTAAACTAACTTGAATTTGACTTTATTCAATTTGAAGTTTTGTATATTTTGTGTTTGTCAGAGGGTATACTTTGATTGGATTTTAGTTGATATCTATTGCAAAATTTAACCTAAATATATAATTCTTAAATAGGAAAAACTGAATGGCCAGAGCTTGTCTTTGTAAAATATTCTATTGCTGCTGCGATCATAGAAAAAGAGAATCCTGATGTGGAAGCTGTCAAGATTCTATCTGGTACACCTAGAATTCTGAATTTTGATATCAATCGAGTTTGGGTTGATGTCAATGTGGAAGAAGTAGTCGTGAAAAGGCCCAAGGTTGGTTAG

mRNA sequence

ATGGCTGAAGAATCTGCTCCAAGAAAAACTGAATGGCCAGAGCTTGTCTTTGTAAAATATTCTATTGCTGCTGCGATCATAGAAAAAGAGAATCCTGATGTGGAAGCTGTCAAGATTCTATCTGGTACACCTAGAATTCTGAATTTTGATATCAATCGAGTTTGGGTTGATGTCAATGTGGAAGAAGTAGTCGTGAAAAGGCCCAAGGTTGGTTAG

Coding sequence (CDS)

ATGGCTGAAGAATCTGCTCCAAGAAAAACTGAATGGCCAGAGCTTGTCTTTGTAAAATATTCTATTGCTGCTGCGATCATAGAAAAAGAGAATCCTGATGTGGAAGCTGTCAAGATTCTATCTGGTACACCTAGAATTCTGAATTTTGATATCAATCGAGTTTGGGTTGATGTCAATGTGGAAGAAGTAGTCGTGAAAAGGCCCAAGGTTGGTTAG

Protein sequence

MAEESAPRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDVNVEEVVVKRPKVG*

Homology

[Close]

BLAST of Chy6G110300 vs. ExPASy Swiss-Prot
Match: P19873 (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 1.9e-09
Identity = 32/67 (47.76%), Postives = 44/67 (65.67%), Query Frame = 0


Query: 5  SAPRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDVNVEEVV 64
          S P K+ WP LV V  S+A AIIE++NP+V+AV +  GTP   +F  NRV + VN   +V
Sbjct: 2  SCPGKSSWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPVTKDFRCNRVRIWVNKRGLV 61

Query: 65 VKRPKVG 72
          V  P++G
Sbjct: 62 VSPPRIG 68

[Close]

BLAST of Chy6G110300 vs. ExPASy Swiss-Prot
Match: Q6XNP7 (Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2)

HSP 1 Score: 52.4 bits (124), Expect = 2.5e-06
Identity = 27/65 (41.54%), Postives = 37/65 (56.92%), Query Frame = 0


Query: 7  PRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDVNVEEVVVK 66
          P K  WPELV     IAA II+ EN +V+A+ +  G P   + + NRV V V+   VV +
Sbjct: 6  PVKNSWPELVGTNGDIAAGIIQTENANVKAIVVKEGLPITQDLNFNRVRVFVDENRVVTQ 65

Query: 67 RPKVG 72
           P +G
Sbjct: 66 VPAIG 70

[Close]

BLAST of Chy6G110300 vs. ExPASy Swiss-Prot
Match: P82381 (Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 4.3e-06
Identity = 26/64 (40.62%), Postives = 37/64 (57.81%), Query Frame = 0


Query: 7  PRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDVNVEEVVVK 66
          P K  WPELV    ++AAA +E+EN +V A+ +  G+    +F  +RVWV VN   VV  
Sbjct: 5  PGKNAWPELVGKSGNMAAATVERENRNVHAIVLKEGSAMTKDFRCDRVWVIVNDHGVVTS 64

Query: 67 RPKV 71
           P +
Sbjct: 65 VPHI 68

[Close]

BLAST of Chy6G110300 vs. ExPASy Swiss-Prot
Match: P80211 (Trypsin/subtilisin inhibitor OS=Amaranthus caudatus OX=3567 PE=1 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 7.3e-06
Identity = 29/64 (45.31%), Postives = 39/64 (60.94%), Query Frame = 0


Query: 7  PRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDVNVEEVVVK 66
          P K EWPELV      AAAIIE+ENP+V ++     +    +F  +RVWV V+   VVV+
Sbjct: 5  PGKQEWPELVGEYGYKAAAIIERENPNVRSIVKHERSGFTKDFRCDRVWVVVDSTGVVVR 64

Query: 67 RPKV 71
           P+V
Sbjct: 65 TPRV 68

[Close]

BLAST of Chy6G110300 vs. ExPASy Swiss-Prot
Match: P24076 (Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 6.2e-05
Identity = 25/63 (39.68%), Postives = 36/63 (57.14%), Query Frame = 0


Query: 9  KTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDVNVEEVVVKRP 68
          K  WP+LV    + A A+IE+ENP V AV +  G+P   +F  +RV V V    +V + P
Sbjct: 6  KRSWPQLVGSTGAAAKAVIERENPRVRAVIVRVGSPVTADFRCDRVRVWVTERGIVARPP 65

Query: 69 KVG 72
           +G
Sbjct: 66 AIG 68

[Close]

BLAST of Chy6G110300 vs. ExPASy TrEMBL
Match: A0A0A0L8E6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G142910 PE=3 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 6.8e-31
Identity = 69/71 (97.18%), Postives = 71/71 (100.00%), Query Frame = 0


Query: 1  MAEESAPRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDVNV 60
          MAEESAPRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVD+NV
Sbjct: 1  MAEESAPRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDINV 60

Query: 61 EEVVVKRPKVG 72
          EEVVVK+PKVG
Sbjct: 61 EEVVVKKPKVG 71

[Close]

BLAST of Chy6G110300 vs. ExPASy TrEMBL
Match: A0A5A7U228 (Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001230 PE=3 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 7.3e-25
Identity = 57/68 (83.82%), Postives = 64/68 (94.12%), Query Frame = 0


Query: 3  EESAPRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDVNVEE 62
          ++SAPRKT+WPELVF+KYSIAAAIIE+ENPDVEAVKILSGTPRILNFD  RVW DVNVE+
Sbjct: 4  DQSAPRKTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTPRILNFDTKRVWCDVNVED 63

Query: 63 VVVKRPKV 71
          V+VK PKV
Sbjct: 64 VIVKMPKV 71

[Close]

BLAST of Chy6G110300 vs. ExPASy TrEMBL
Match: A0A5A7U4L9 (Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001240 PE=3 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 1.5e-22
Identity = 56/69 (81.16%), Postives = 61/69 (88.41%), Query Frame = 0


Query: 3  EESAPRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDVNVEE 62
          ++SAPRKT+WPELVFVKYS AAAIIEKENPDV+AVKILSG  RI NFD  RVW DVNVE+
Sbjct: 4  DQSAPRKTQWPELVFVKYSTAAAIIEKENPDVKAVKILSGRFRIQNFDTKRVWCDVNVED 63

Query: 63 VVVKRPKVG 72
          VVVK PKVG
Sbjct: 64 VVVKTPKVG 72

[Close]

BLAST of Chy6G110300 vs. ExPASy TrEMBL
Match: A0A5A7U4U0 (Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001250 PE=3 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 3.2e-20
Identity = 52/71 (73.24%), Postives = 60/71 (84.51%), Query Frame = 0


Query: 1  MAEESAPRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDVNV 60
          MAEESA RKT WPELVFV++S AA IIEKENPDV+A+KIL+ +PRI NFD +RVWVD NV
Sbjct: 1  MAEESAQRKTRWPELVFVEFSTAARIIEKENPDVKAIKILADSPRIENFDPSRVWVDCNV 60

Query: 61 EEVVVKRPKVG 72
          E+ VVK P VG
Sbjct: 61 EDKVVKVPSVG 71

[Close]

BLAST of Chy6G110300 vs. ExPASy TrEMBL
Match: A0A0A0L785 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G141900 PE=3 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 3.2e-20
Identity = 52/71 (73.24%), Postives = 58/71 (81.69%), Query Frame = 0


Query: 1  MAEESAPRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDVNV 60
          MAEESA RKT WPELVFV +  AA IIEKENPDV+A+KIL  +PRI NFDI+RVWVD N+
Sbjct: 1  MAEESAQRKTRWPELVFVNFCTAARIIEKENPDVKAIKILVDSPRIQNFDISRVWVDCNI 60

Query: 61 EEVVVKRPKVG 72
          EE VVK P VG
Sbjct: 61 EERVVKVPSVG 71

[Close]

BLAST of Chy6G110300 vs. NCBI nr
Match: KGN56897.1 (hypothetical protein Csa_010510 [Cucumis sativus])

HSP 1 Score: 142 bits (358), Expect = 1.45e-42
Identity = 69/71 (97.18%), Postives = 71/71 (100.00%), Query Frame = 0


Query: 1  MAEESAPRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDVNV 60
          MAEESAPRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVD+NV
Sbjct: 1  MAEESAPRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDINV 60

Query: 61 EEVVVKRPKVG 71
          EEVVVK+PKVG
Sbjct: 61 EEVVVKKPKVG 71

[Close]

BLAST of Chy6G110300 vs. NCBI nr
Match: KAA0049288.1 (Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17270.1 Protease inhibitor protein [Cucumis melo var. makuwa])

HSP 1 Score: 122 bits (307), Expect = 5.60e-34
Identity = 60/71 (84.51%), Postives = 66/71 (92.96%), Query Frame = 0


Query: 1  MAEE-SAPRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDVN 60
          MAE+ SAPRKT+WPELVF+KYSIAAAIIE+ENPDVEAVKILSGTPRILNFD  RVW DVN
Sbjct: 1  MAEDQSAPRKTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTPRILNFDTKRVWCDVN 60

Query: 61 VEEVVVKRPKV 70
          VE+V+VK PKV
Sbjct: 61 VEDVIVKMPKV 71

[Close]

BLAST of Chy6G110300 vs. NCBI nr
Match: KAA0049287.1 (Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17271.1 Protease inhibitor protein [Cucumis melo var. makuwa])

HSP 1 Score: 115 bits (287), Expect = 1.02e-31
Identity = 59/72 (81.94%), Postives = 63/72 (87.50%), Query Frame = 0


Query: 1  MAEE-SAPRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDVN 60
          MAE+ SAPRKT+WPELVFVKYS AAAIIEKENPDV+AVKILSG  RI NFD  RVW DVN
Sbjct: 1  MAEDQSAPRKTQWPELVFVKYSTAAAIIEKENPDVKAVKILSGRFRIQNFDTKRVWCDVN 60

Query: 61 VEEVVVKRPKVG 71
          VE+VVVK PKVG
Sbjct: 61 VEDVVVKTPKVG 72

[Close]

BLAST of Chy6G110300 vs. NCBI nr
Match: KAA0049286.1 (Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17272.1 Protease inhibitor protein [Cucumis melo var. makuwa])

HSP 1 Score: 107 bits (266), Expect = 1.59e-28
Identity = 52/71 (73.24%), Postives = 60/71 (84.51%), Query Frame = 0


Query: 1  MAEESAPRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDVNV 60
          MAEESA RKT WPELVFV++S AA IIEKENPDV+A+KIL+ +PRI NFD +RVWVD NV
Sbjct: 1  MAEESAQRKTRWPELVFVEFSTAARIIEKENPDVKAIKILADSPRIENFDPSRVWVDCNV 60

Query: 61 EEVVVKRPKVG 71
          E+ VVK P VG
Sbjct: 61 EDKVVKVPSVG 71

[Close]

BLAST of Chy6G110300 vs. NCBI nr
Match: KAE8650337.1 (hypothetical protein Csa_010581 [Cucumis sativus])

HSP 1 Score: 103 bits (256), Expect = 1.36e-25
Identity = 50/68 (73.53%), Postives = 56/68 (82.35%), Query Frame = 0


Query: 1  MAEESAPRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDVNV 60
          MAEESA RKT WPELVFV +  AA IIEKENPDV+A+KIL  +PRI NFDI+RVWVD N+
Sbjct: 1  MAEESAQRKTRWPELVFVNFCTAARIIEKENPDVKAIKILVDSPRIQNFDISRVWVDCNI 60

Query: 61 EEVVVKRP 68
          EE VVK P
Sbjct: 61 EERVVKVP 68

[Close]

BLAST of Chy6G110300 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 60.8 bits (146), Expect = 5.0e-10
Identity = 31/65 (47.69%), Postives = 38/65 (58.46%), Query Frame = 0


Query: 7  PRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDVNVEEVVVK 66
          PRK  WPEL       AA +IE+ENP V A  IL G+P   +F  +RV V V+   +VVK
Sbjct: 6  PRKNSWPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTADFRCDRVRVFVDGNRIVVK 65

Query: 67 RPKVG 72
           PK G
Sbjct: 66 TPKSG 70

[Close]

BLAST of Chy6G110300 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 50.4 bits (119), Expect = 6.8e-07
Identity = 29/65 (44.62%), Postives = 40/65 (61.54%), Query Frame = 0


Query: 7  PRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDVNVEEVVVK 66
          P K+ WPEL+  K   A  +IE+ENP ++AV IL GT     F  +RV+V VN   +VV+
Sbjct: 35 PGKSSWPELLGAKGEDAKEVIERENPKMKAVIILDGTVVPEIFICSRVYVWVNDCGIVVQ 94

Query: 67 RPKVG 72
           P +G
Sbjct: 95 IPIIG 99

[Close]

BLAST of Chy6G110300 vs. TAIR 10
Match: AT3G46860.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 48.5 bits (114), Expect = 2.6e-06
Identity = 26/64 (40.62%), Postives = 35/64 (54.69%), Query Frame = 0


Query: 8  RKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDVNVEEVVVKR 67
          +K  WPELV     +A   IE+ENP+V A+ +  G  RI NF  NRV+V +     V   
Sbjct: 22 KKYRWPELVGKNGQLAKMTIERENPNVLAIVLRYGEKRIENFCCNRVFVYLGSNGQVADA 81

Query: 68 PKVG 72
          P +G
Sbjct: 82 PMIG 85

The following BLAST results are available for this feature:

BLAST of Chy6G110300 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P19873	1.9e-09	47.76	Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]
Q6XNP7	2.5e-06	41.54	Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2	[more]
P82381	4.3e-06	40.63	Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1	[more]
P80211	7.3e-06	45.31	Trypsin/subtilisin inhibitor OS=Amaranthus caudatus OX=3567 PE=1 SV=1	[more]
P24076	6.2e-05	39.68	Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1	[more]

BLAST of Chy6G110300 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L8E6	6.8e-31	97.18	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G142910 PE=3 SV=1	[more]
A0A5A7U228	7.3e-25	83.82	Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A5A7U4L9	1.5e-22	81.16	Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A5A7U4U0	3.2e-20	73.24	Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A0A0L785	3.2e-20	73.24	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G141900 PE=3 SV=1	[more]

BLAST of Chy6G110300 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN56897.1	1.45e-42	97.18	hypothetical protein Csa_010510 [Cucumis sativus]	[more]
KAA0049288.1	5.60e-34	84.51	Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17270.1 Protease inhib...	[more]
KAA0049287.1	1.02e-31	81.94	Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17271.1 Protease inhib...	[more]
KAA0049286.1	1.59e-28	73.24	Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17272.1 Protease inhib...	[more]
KAE8650337.1	1.36e-25	73.53	hypothetical protein Csa_010581 [Cucumis sativus]	[more]

BLAST of Chy6G110300 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38870.1	5.0e-10	47.69	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT5G43580.1	6.8e-07	44.62	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT3G46860.1	2.6e-06	40.63	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 9..71 e-value: 1.1E-16 score: 61.3
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 3..71
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 4..71 e-value: 3.6E-20 score: 74.1
None	No IPR available	PANTHER	PTHR33091:SF62	PROTEASE INHIBITOR PROTEIN	coord: 3..71
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 6..71

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy6G110300.1	Chy6G110300.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity