Chy6G108150 (gene) Cucumber (hystrix) v1

Overview
NameChy6G108150
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionEnt-kaurenoic acid oxidase 2-like
LocationchrH06: 1915486 .. 1917691 (-)
RNA-Seq ExpressionChy6G108150
SyntenyChy6G108150
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATAATGAGGAGATTAGAAATCTAATTCTTTTAATTAATTATAGGTTTGGGAAAATCGGAGTGTACAAAATTCATTTGTTTGGAAACCCTAGCGTTGTCGTGACGACGCCGGAGACATGCCGGAAAGTTCTGACCGACGACGAAGCCTTTCAGCCCGGTTGGCCACGAGCAGCAGTAGAGCTCATTGGAAAGAAGTCCTTCATCGAAATGCCGGTCGAGGAACACAAGCGCCTCCGACGCTTAACCTCTGCTCCGGTGAACGGCTTCGAAGCTTTGAGCAACTACATTCCTTACATTGAAGAGAATGTATTGAAATCATTGGATAAATGGTCGAATATGGGACCAATCGAATTCTTAACTCAACTTCGTAAGCTCACATTCACCATTATCATGTACATTTTCCTTAGCGCGGAGAGTGAATCGGTAATGGAATCGTTAGAGAAGGAGTATACTCGGCTTAACTATGGTGTTCGAGCCCTGAGAATTAACATTCCTGGATTTGCTTACCACAAAGCACTCAAGGTGTTTGATAATTTGCCTCAATTAATTACGAAAAATGTACTTTGGTTTCTGGAATTTGTTTGATCAATTAAACACCATCGGTTGAATGTTTAGGCTCGGAAGAATCTTGTGGCTGCGTTTCAAGGAATTGTGACGGAGCGAAGAAAGCGAAGATTGGGCAATTGGGCGCCGAAGAGGAAGGACATGATGGACGCTCTGATTGATGTTGAAGATGAAAATGGCAGAAAGTTGACTGATGAGGAGATCATTGACATTCTGGTGATGTACTTGAATGCAGGTCATGAATCTTCAGGCCATACCATGATGTGGGCAACTATTTTGTTGAATCAACACCCAGAAGTTCTCAAGAAAGCCAAGGTATATACAAAATTAGGATCTTAATTAGATTGTTTGGTTACTCAAAATCATGTCATATTTCATACTTAGTGACAAAATTCGTGTGGTATCATATAAGATCAATCATGATAAAAGTTGGTTTAATAAACTGAACAGGAGGAACAGGAGGAGATTGTGAGAAGGAGGCCTGCAGGTCAAAAGGGATTGACATTGAAAGAATGCAGGGATATGGAATATCTCTCCAAAGTGGTCGATGAAACTCTTCGCTACGTATCATTCTCCCTTGTTGTCTTCAGAGAAGCACAAATGGATGTCAATCTCAATGGTATACCTATCTCTCTCTCTCTCTCTCTCTCTTTTTGATTGTCGTATTAGCAGTTTACATTTTAACAATGGAGGGTCAAACCATCAACTCTACAGATTAACTATTCTCAAATTGACAATATAGAAATCGCTAGAGTAGTATTTATTGAATGTTTTAGCCTCTCTCTTATTTGAATTGGAACAATGTGGTCAGTCGTTTCATACCATTACATATTTGCGAGAGAGATCGAAAATTTGGTTTAACCATGCAATTGAGAAATTATTCAATAAATCTGCTACAACGTGGCTAGCAATTTTGCACTATTGAATATATGAAAGATGAACTATAAATGTATTTCCAACTTATACCTAGAGGTTATTAGAAACAGTCAGGTTTTCAAATCCATGAACAATTTTTCCTATGCATCCAAGTGTTAGGAGATAGATGGGCACTGAATAATTATTTCCTCTTGTTTATTTCTCCATTGTATGAATGATGTTTGTGTGATTGCAGGCTATCTAATTCCCAAGGGTTGGAAAGTGCTGGCTTGGTTCAGAAGCATTCACTATGACAATGAAGTTTACCCAGACCCAAAGAAATTTGATCCTTCAAGATGGGATGTAAGTTAAAACTCATAGTTTCAATATCCTTTTTACAAAAATTAAAGTTATATCACATCAAATGAAATCCATCTGATATCCACACTGTGTTTGGTATTTGTATAAAGGGATTTATCCCAAAAGCAGGAGAGTTTCTTCCATTTGGAGCAGGAAGCAGGCTGTGCCCTGGAAATGATCTAGCCAAATTGGAGATTTGTATTTTCATTCACTATTTTCTCCTCAATTACAAGTAAGTTCACTTGGAAAACACAGAATATAACGAGTTTTTTTGTATTAACAATTTGTGTTGTTAATGGGGTTGATTTTTTTCTACAGGCTTGAATGGCTGACCCCTGATTGTCAAATCTTGTATCTGCCCCATTCAAGGCCAAAGGACAATTGCTTGGCTAAAATCAGTAAGAATTCCAGCACTGTTGTGTGA

mRNA sequence

ATGAATAATGAGGAGATTAGAAATCTAATTCTTTTAATTAATTATAGGTTTGGGAAAATCGGAGTGTACAAAATTCATTTGTTTGGAAACCCTAGCGTTGTCGTGACGACGCCGGAGACATGCCGGAAAGTTCTGACCGACGACGAAGCCTTTCAGCCCGGTTGGCCACGAGCAGCAGTAGAGCTCATTGGAAAGAAGTCCTTCATCGAAATGCCGGTCGAGGAACACAAGCGCCTCCGACGCTTAACCTCTGCTCCGGTGAACGGCTTCGAAGCTTTGAGCAACTACATTCCTTACATTGAAGAGAATGTATTGAAATCATTGGATAAATGGTCGAATATGGGACCAATCGAATTCTTAACTCAACTTCGTAAGCTCACATTCACCATTATCATGTACATTTTCCTTAGCGCGGAGAGTGAATCGGTAATGGAATCGTTAGAGAAGGAGTATACTCGGCTTAACTATGGTGTTCGAGCCCTGAGAATTAACATTCCTGGATTTGCTTACCACAAAGCACTCAAGGCTCGGAAGAATCTTGTGGCTGCGTTTCAAGGAATTGTGACGGAGCGAAGAAAGCGAAGATTGGGCAATTGGGCGCCGAAGAGGAAGGACATGATGGACGCTCTGATTGATGTTGAAGATGAAAATGGCAGAAAGTTGACTGATGAGGAGATCATTGACATTCTGGTGATGTACTTGAATGCAGGTCATGAATCTTCAGGCCATACCATGATGTGGGCAACTATTTTGTTGAATCAACACCCAGAAGTTCTCAAGAAAGCCAAGGAGGAACAGGAGGAGATTGTGAGAAGGAGGCCTGCAGGTCAAAAGGGATTGACATTGAAAGAATGCAGGGATATGGAATATCTCTCCAAAGTGGTCGATGAAACTCTTCGCTACGTATCATTCTCCCTTGTTGTCTTCAGAGAAGCACAAATGGATGTCAATCTCAATGGCTATCTAATTCCCAAGGGTTGGAAAGTGCTGGCTTGGTTCAGAAGCATTCACTATGACAATGAAGTTTACCCAGACCCAAAGAAATTTGATCCTTCAAGATGGGATGGATTTATCCCAAAAGCAGGAGAGTTTCTTCCATTTGGAGCAGGAAGCAGGCTGTGCCCTGGAAATGATCTAGCCAAATTGGAGATTTGTATTTTCATTCACTATTTTCTCCTCAATTACAAGCTTGAATGGCTGACCCCTGATTGTCAAATCTTGTATCTGCCCCATTCAAGGCCAAAGGACAATTGCTTGGCTAAAATCAGTAAGAATTCCAGCACTGTTGTGTGA

Coding sequence (CDS)

ATGAATAATGAGGAGATTAGAAATCTAATTCTTTTAATTAATTATAGGTTTGGGAAAATCGGAGTGTACAAAATTCATTTGTTTGGAAACCCTAGCGTTGTCGTGACGACGCCGGAGACATGCCGGAAAGTTCTGACCGACGACGAAGCCTTTCAGCCCGGTTGGCCACGAGCAGCAGTAGAGCTCATTGGAAAGAAGTCCTTCATCGAAATGCCGGTCGAGGAACACAAGCGCCTCCGACGCTTAACCTCTGCTCCGGTGAACGGCTTCGAAGCTTTGAGCAACTACATTCCTTACATTGAAGAGAATGTATTGAAATCATTGGATAAATGGTCGAATATGGGACCAATCGAATTCTTAACTCAACTTCGTAAGCTCACATTCACCATTATCATGTACATTTTCCTTAGCGCGGAGAGTGAATCGGTAATGGAATCGTTAGAGAAGGAGTATACTCGGCTTAACTATGGTGTTCGAGCCCTGAGAATTAACATTCCTGGATTTGCTTACCACAAAGCACTCAAGGCTCGGAAGAATCTTGTGGCTGCGTTTCAAGGAATTGTGACGGAGCGAAGAAAGCGAAGATTGGGCAATTGGGCGCCGAAGAGGAAGGACATGATGGACGCTCTGATTGATGTTGAAGATGAAAATGGCAGAAAGTTGACTGATGAGGAGATCATTGACATTCTGGTGATGTACTTGAATGCAGGTCATGAATCTTCAGGCCATACCATGATGTGGGCAACTATTTTGTTGAATCAACACCCAGAAGTTCTCAAGAAAGCCAAGGAGGAACAGGAGGAGATTGTGAGAAGGAGGCCTGCAGGTCAAAAGGGATTGACATTGAAAGAATGCAGGGATATGGAATATCTCTCCAAAGTGGTCGATGAAACTCTTCGCTACGTATCATTCTCCCTTGTTGTCTTCAGAGAAGCACAAATGGATGTCAATCTCAATGGCTATCTAATTCCCAAGGGTTGGAAAGTGCTGGCTTGGTTCAGAAGCATTCACTATGACAATGAAGTTTACCCAGACCCAAAGAAATTTGATCCTTCAAGATGGGATGGATTTATCCCAAAAGCAGGAGAGTTTCTTCCATTTGGAGCAGGAAGCAGGCTGTGCCCTGGAAATGATCTAGCCAAATTGGAGATTTGTATTTTCATTCACTATTTTCTCCTCAATTACAAGCTTGAATGGCTGACCCCTGATTGTCAAATCTTGTATCTGCCCCATTCAAGGCCAAAGGACAATTGCTTGGCTAAAATCAGTAAGAATTCCAGCACTGTTGTGTGA

Protein sequence

MNNEEIRNLILLINYRFGKIGVYKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEEHKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIFLSAESESVMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRRLGNWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQHPEVLKKAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMDVNLNGYLIPKGWKVLAWFRSIHYDNEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCPGNDLAKLEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISKNSSTVV*
Homology
[Close]
BLAST of Chy6G108150 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 622.1 bits (1603), Expect = 4.8e-177
Identity = 286/409 (69.93%), Postives = 350/409 (85.57%), Query Frame = 0 

Query: 16  RFGKIGVYKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEE 75
           R+G  G+YK H+FGNPS++VTT +TCR+VLTDD+AF+PGWP + +ELIG+KSF+ +  EE
Sbjct: 80  RYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEE 139

Query: 76  HKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIF 135
           HKRLRRLT+APVNG EALS YIPYIEENV+  LDKW+ MG  EFLT LRKLTF IIMYIF
Sbjct: 140 HKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGEFEFLTHLRKLTFRIIMYIF 199

Query: 136 LSAESESVMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRR 195
           LS+ESE+VM++LE+EYT LNYGVRA+ +NIPGFAYH+ALKARK LVAAFQ IVTERR +R
Sbjct: 200 LSSESENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAFQSIVTERRNQR 259

Query: 196 LGNWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQH 255
             N    +KDM+D L++V+DE+G+ L DEEIID+L+MYLNAGHESSGHT+MWAT+ L +H
Sbjct: 260 KQNILSNKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQEH 319

Query: 256 PEVLKKAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMD 315
           PEVL++AK EQE I++ RP GQKGL+LKE R ME+LS+VVDETLR ++FSL  FREA+ D
Sbjct: 320 PEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVITFSLTAFREAKTD 379

Query: 316 VNLNGYLIPKGWKVLAWFRSIHYDNEVYPDPKKFDPSRWD-GFIPKAGEFLPFGAGSRLC 375
           V +NGYLIPKGWKVL WFR +H D EV+PDP+KFDP+RWD GF+PKAG FLPFGAGS LC
Sbjct: 380 VEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGAFLPFGAGSHLC 439

Query: 376 PGNDLAKLEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKIS 424
           PGNDLAKLEI IF+H+FLL Y+++   P+C ++YLPH+RP DNCLA+IS
Sbjct: 440 PGNDLAKLEISIFLHHFLLKYQVKRSNPECPVMYLPHTRPTDNCLARIS 488

[Close]
BLAST of Chy6G108150 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 606.3 bits (1562), Expect = 2.7e-172
Identity = 283/409 (69.19%), Postives = 344/409 (84.11%), Query Frame = 0 

Query: 16  RFGKIGVYKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEE 75
           R+G+ G+YK H+FG P V+VTTPETCR+VLTDD+AF  GWP++ ++LIG+KSF+ +  EE
Sbjct: 79  RYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSFVGISFEE 138

Query: 76  HKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIF 135
           HKRLRRLTSAPVNG EALS YI +IEE V   L+KWS MG IEFL+ LRKLTF +IMYIF
Sbjct: 139 HKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGEIEFLSHLRKLTFKVIMYIF 198

Query: 136 LSAESESVMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRR 195
           LS+ESE VM+SLE+EYT LNYGVRA+ IN+PGFAYH+ALKARK LVAAFQ IVT RR +R
Sbjct: 199 LSSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAFQSIVTNRRNQR 258

Query: 196 LGNWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQH 255
             N +  RKDM+D LIDV+DENGR L DEEIID+L+MYLNAGHESSGH  MWATIL+ +H
Sbjct: 259 KQNISSNRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGHLTMWATILMQEH 318

Query: 256 PEVLKKAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMD 315
           P +L+KAKEEQE IV++R  GQK LTLKE R+M YLS+V+DETLR ++FSL  FREA+ D
Sbjct: 319 PMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVITFSLTAFREAKSD 378

Query: 316 VNLNGYLIPKGWKVLAWFRSIHYDNEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCP 375
           V ++GY+IPKGWKVL WFR++H D E+YPDPKKFDPSRW+G+ PKAG FLPFG GS LCP
Sbjct: 379 VQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTFLPFGLGSHLCP 438

Query: 376 GNDLAKLEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISK 425
           GNDLAKLEI IF+H+FLL Y++E   P C +++LPH+RPKDNCLA+I++
Sbjct: 439 GNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARITR 486

[Close]
BLAST of Chy6G108150 vs. ExPASy Swiss-Prot
Match: Q9AXH9 (Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 5.5e-149
Identity = 248/418 (59.33%), Postives = 321/418 (76.79%), Query Frame = 0 

Query: 16  RFGKIGVYKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEE 75
           RFG+ G+Y+  +F +P+++ TTPE C++VL DD+AF  GWP+A V LIG KSF+ M  +E
Sbjct: 80  RFGRAGLYRAFMFSSPTIMATTPEACKQVLMDDDAFVTGWPKATVALIGPKSFVNMGYDE 139

Query: 76  HKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNM---GPIEFLTQLRKLTFTIIM 135
           H+RLR+LT+AP+NGF+AL++Y+ +I++ V+ +L  WS     G  EFLT+LR++TF II+
Sbjct: 140 HRRLRKLTAAPINGFDALTSYLGFIDDTVVTTLRGWSERGGDGHFEFLTELRRMTFRIIV 199

Query: 136 YIFLSAESESVMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERR 195
            IF+    E     LE+ YT LNYG+RA+ I++PGFAYHKA++AR+ LVAA Q ++ ERR
Sbjct: 200 QIFMGGADERTAAELERTYTELNYGMRAMAIDLPGFAYHKAIRARRRLVAALQRVLDERR 259

Query: 196 KR--RLGNWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATI 255
            R  +    A    DMMD LI VEDE GR+L D+EIID+LVMYLNAGHESSGH  MWAT+
Sbjct: 260 ARGGKTAAGAAAPVDMMDRLIAVEDEGGRRLQDDEIIDVLVMYLNAGHESSGHITMWATV 319

Query: 256 LLNQHPEVLKKAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFR 315
            L ++PE+L KAK EQE I+R  P GQKGLTL++ R M YLS+VVDETLR+V+ S V FR
Sbjct: 320 FLQENPEILAKAKAEQEAIMRSIPPGQKGLTLRDFRKMAYLSQVVDETLRFVNISFVSFR 379

Query: 316 EAQMDVNLNGYLIPKGWKVLAWFRSIHYDNEVYPDPKKFDPSRWDGFIPKAGEFLPFGAG 375
           +A  DV +NGYLIPKGWKV  W+RS+H D +VYPDPKKFDPSRW+G  P+AG FLPFG G
Sbjct: 380 QATRDVFVNGYLIPKGWKVQLWYRSVHMDPQVYPDPKKFDPSRWEGPPPRAGTFLPFGLG 439

Query: 376 SRLCPGNDLAKLEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISKNSST 429
           +RLCPGNDLAKLEI +F+H+FLL YKL    P+C++ YLPH RP DNCLAKI++ SS+
Sbjct: 440 TRLCPGNDLAKLEISVFLHHFLLGYKLTRKNPNCRVRYLPHPRPVDNCLAKITRLSSS 497

[Close]
BLAST of Chy6G108150 vs. ExPASy Swiss-Prot
Match: Q43246 (Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 5.7e-146
Identity = 238/413 (57.63%), Postives = 314/413 (76.03%), Query Frame = 0 

Query: 16  RFGKIGVYKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEE 75
           RFG+ GVY+  +F +P+V+VTT E C++VL DD+AF  GWP+A V L+G +SF+ MP +E
Sbjct: 107 RFGRTGVYRSFMFSSPTVLVTTAEGCKQVLMDDDAFVTGWPKATVALVGPRSFVAMPYDE 166

Query: 76  HKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNM-GPIEFLTQLRKLTFTIIMYI 135
           H+R+R+LT+AP+NGF+AL+ Y+P+I+  V  SL  W++  G +EFLT+LR++TF II+ I
Sbjct: 167 HRRIRKLTAAPINGFDALTGYLPFIDRTVTSSLRAWADHGGSVEFLTELRRMTFKIIVQI 226

Query: 136 FLSAESESVMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKR 195
           FL    ++   +LE+ YT LNYG+RA+ IN+PGFAY  AL+AR+ LVA  QG++ ERR  
Sbjct: 227 FLGGADQATTRALERSYTELNYGMRAMAINLPGFAYRGALRARRRLVAVLQGVLDERRAA 286

Query: 196 RLGNWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQ 255
           R    +    DMMD LI+ +DE GR L D+EIID+LVMYLNAGHESSGH  MWAT+ L +
Sbjct: 287 RAKGVSGGGVDMMDRLIEAQDERGRHLDDDEIIDVLVMYLNAGHESSGHITMWATVFLQE 346

Query: 256 HPEVLKKAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQM 315
           +P++  +AK EQE I+R  P+ Q+GLTL++ R MEYLS+V+DETLR V+ S V FR+A  
Sbjct: 347 NPDMFARAKAEQEAIMRSIPSSQRGLTLRDFRKMEYLSQVIDETLRLVNISFVSFRQATR 406

Query: 316 DVNLNGYLIPKGWKVLAWFRSIHYDNEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLC 375
           DV +NGYLIPKGWKV  W+RS+H D +VYPDP KFDPSRW+G  P+AG FL FG G+RLC
Sbjct: 407 DVFVNGYLIPKGWKVQLWYRSVHMDPQVYPDPTKFDPSRWEGHSPRAGTFLAFGLGARLC 466

Query: 376 PGNDLAKLEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISKNSS 428
           PGNDLAKLEI +F+H+FLL YKL    P C++ YLPH RP DNCLAKI++  S
Sbjct: 467 PGNDLAKLEISVFLHHFLLGYKLARTNPRCRVRYLPHPRPVDNCLAKITRVGS 519

[Close]
BLAST of Chy6G108150 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 2.8e-145
Identity = 235/414 (56.76%), Postives = 318/414 (76.81%), Query Frame = 0 

Query: 16  RFGKIGVYKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEE 75
           RFG+ GVY+  +F +P+++  TPE C++VL DDE F  GWP+A V LIG KSF+ M  ++
Sbjct: 90  RFGRTGVYRTFMFSSPTILAVTPEACKQVLMDDEGFVTGWPKATVTLIGPKSFVNMSYDD 149

Query: 76  HKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSN--MGPIEFLTQLRKLTFTIIMY 135
           H+R+R+LT+AP+NGF+AL+ Y+ +I++ V+ SL +WS+   G +EFLT+LR++TF II+ 
Sbjct: 150 HRRIRKLTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVEFLTELRRMTFKIIVQ 209

Query: 136 IFLSAESESVMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRK 195
           IF+S   ++ ME+LE+ YT LNYG+RA+ IN+PGFAY++AL+AR+ LV+  QG++  RR 
Sbjct: 210 IFMSGADDATMEALERSYTDLNYGMRAMAINLPGFAYYRALRARRKLVSVLQGVLDGRRA 269

Query: 196 RRL-GNWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILL 255
               G       DMMD LI+ EDE GR+L D+EI+D+L+MYLNAGHESSGH  MWAT+ L
Sbjct: 270 AAAKGFKRSGAMDMMDRLIEAEDERGRRLADDEIVDVLIMYLNAGHESSGHITMWATVFL 329

Query: 256 NQHPEVLKKAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREA 315
            ++P++  +AK EQEEI+R  PA Q GLTL++ + M +LS+VVDETLR V+ S V FR+A
Sbjct: 330 QENPDIFARAKAEQEEIMRSIPATQNGLTLRDFKKMHFLSQVVDETLRCVNISFVSFRQA 389

Query: 316 QMDVNLNGYLIPKGWKVLAWFRSIHYDNEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSR 375
             D+ +NGYLIPKGWKV  W+RS+H D++VYPDPK F+PSRW+G  PKAG FLPFG G+R
Sbjct: 390 TRDIFVNGYLIPKGWKVQLWYRSVHMDDQVYPDPKMFNPSRWEGPPPKAGTFLPFGLGAR 449

Query: 376 LCPGNDLAKLEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISKNS 427
           LCPGNDLAKLEI +F+H+FLL YKL+   P C++ YLPH RP DNCLA I+K S
Sbjct: 450 LCPGNDLAKLEISVFLHHFLLGYKLKRANPKCRVRYLPHPRPVDNCLATITKVS 503

[Close]
BLAST of Chy6G108150 vs. ExPASy TrEMBL
Match: A0A5D3CZZ0 (Ent-kaurenoic acid oxidase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003480 PE=3 SV=1)

HSP 1 Score: 836.6 bits (2160), Expect = 4.5e-239
Identity = 406/415 (97.83%), Postives = 410/415 (98.80%), Query Frame = 0 

Query: 16  RFGKIGVYKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEE 75
           RFGKIGVYK+HLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEE
Sbjct: 84  RFGKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEE 143

Query: 76  HKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIF 135
           HKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWS MGPIEFLTQLRKLTFTIIMYIF
Sbjct: 144 HKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSTMGPIEFLTQLRKLTFTIIMYIF 203

Query: 136 LSAESESVMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRR 195
           LSAESESVMESLEKEYTRLNYGVRALRIN+PGFAYHKALKARKNLVAA QGIVTERRKRR
Sbjct: 204 LSAESESVMESLEKEYTRLNYGVRALRINLPGFAYHKALKARKNLVAALQGIVTERRKRR 263

Query: 196 LGNWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQH 255
           LG WAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQH
Sbjct: 264 LGKWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQH 323

Query: 256 PEVLKKAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMD 315
           PEVLKKAKEEQEEIVRRRP GQKGLTLKECR+MEYLSKVVDETLRYVSFSLVVFREAQMD
Sbjct: 324 PEVLKKAKEEQEEIVRRRPPGQKGLTLKECREMEYLSKVVDETLRYVSFSLVVFREAQMD 383

Query: 316 VNLNGYLIPKGWKVLAWFRSIHYDNEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCP 375
           VNLNGYLIPKGWKVLAWFRSIHYD+EVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCP
Sbjct: 384 VNLNGYLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCP 443

Query: 376 GNDLAKLEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISKNSSTVV 431
           GNDLAKLEICIFIHYFLLNYKLEWLTPDC ILYLPHSRPKDNCLAKISKNSSTVV
Sbjct: 444 GNDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSRPKDNCLAKISKNSSTVV 498

[Close]
BLAST of Chy6G108150 vs. ExPASy TrEMBL
Match: A0A5A7U100 (Ent-kaurenoic acid oxidase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G003090 PE=3 SV=1)

HSP 1 Score: 835.1 bits (2156), Expect = 1.3e-238
Identity = 405/415 (97.59%), Postives = 409/415 (98.55%), Query Frame = 0 

Query: 16  RFGKIGVYKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEE 75
           RFGKIGVYK+HLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEE
Sbjct: 38  RFGKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEE 97

Query: 76  HKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIF 135
           HKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWS MGPIEFLTQLRKLTFTIIMYIF
Sbjct: 98  HKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSTMGPIEFLTQLRKLTFTIIMYIF 157

Query: 136 LSAESESVMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRR 195
           LSAESESVMESLEKEYTRLNYGVRALRIN+PGFAYHKALKARKNLVAA QGIVTERRKRR
Sbjct: 158 LSAESESVMESLEKEYTRLNYGVRALRINLPGFAYHKALKARKNLVAALQGIVTERRKRR 217

Query: 196 LGNWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQH 255
           LG WAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQH
Sbjct: 218 LGKWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQH 277

Query: 256 PEVLKKAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMD 315
           PEVLKKAKEEQEEIVRRRP GQKGLTLKECR+MEYLSKVVDETLRYVSFSLV FREAQMD
Sbjct: 278 PEVLKKAKEEQEEIVRRRPPGQKGLTLKECREMEYLSKVVDETLRYVSFSLVAFREAQMD 337

Query: 316 VNLNGYLIPKGWKVLAWFRSIHYDNEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCP 375
           VNLNGYLIPKGWKVLAWFRSIHYD+EVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCP
Sbjct: 338 VNLNGYLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCP 397

Query: 376 GNDLAKLEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISKNSSTVV 431
           GNDLAKLEICIFIHYFLLNYKLEWLTPDC ILYLPHSRPKDNCLAKISKNSSTVV
Sbjct: 398 GNDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSRPKDNCLAKISKNSSTVV 452

[Close]
BLAST of Chy6G108150 vs. ExPASy TrEMBL
Match: A0A1S3AWI4 (ent-kaurenoic acid oxidase 2-like OS=Cucumis melo OX=3656 GN=LOC103483403 PE=3 SV=1)

HSP 1 Score: 835.1 bits (2156), Expect = 1.3e-238
Identity = 405/414 (97.83%), Postives = 409/414 (98.79%), Query Frame = 0 

Query: 16  RFGKIGVYKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEE 75
           RFGKIGVYK+HLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEE
Sbjct: 84  RFGKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEE 143

Query: 76  HKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIF 135
           HKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWS MGPIEFLTQLRKLTFTIIMYIF
Sbjct: 144 HKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSTMGPIEFLTQLRKLTFTIIMYIF 203

Query: 136 LSAESESVMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRR 195
           LSAESESVMESLEKEYTRLNYGVRALRIN+PGFAYHKALKARKNLVAA QGIVTERRKRR
Sbjct: 204 LSAESESVMESLEKEYTRLNYGVRALRINLPGFAYHKALKARKNLVAALQGIVTERRKRR 263

Query: 196 LGNWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQH 255
           LG WAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQH
Sbjct: 264 LGKWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQH 323

Query: 256 PEVLKKAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMD 315
           PEVLKKAKEEQEEIVRRRP GQKGLTLKECR+MEYLSKVVDETLRYVSFSLVVFREAQMD
Sbjct: 324 PEVLKKAKEEQEEIVRRRPPGQKGLTLKECREMEYLSKVVDETLRYVSFSLVVFREAQMD 383

Query: 316 VNLNGYLIPKGWKVLAWFRSIHYDNEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCP 375
           VNLNGYLIPKGWKVLAWFRSIHYD+EVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCP
Sbjct: 384 VNLNGYLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCP 443

Query: 376 GNDLAKLEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISKNSSTV 430
           GNDLAKLEICIFIHYFLLNYKLEWLTPDC ILYLPHSRPKDNCLAKISKNSSTV
Sbjct: 444 GNDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSRPKDNCLAKISKNSSTV 497

[Close]
BLAST of Chy6G108150 vs. ExPASy TrEMBL
Match: A0A6J1E9S4 (ent-kaurenoic acid oxidase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111432076 PE=3 SV=1)

HSP 1 Score: 788.9 bits (2036), Expect = 1.1e-224
Identity = 372/412 (90.29%), Postives = 397/412 (96.36%), Query Frame = 0 

Query: 16  RFGKIGVYKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEE 75
           R+GK GVYK+HLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAA+ELIG+KSFI+MP EE
Sbjct: 82  RYGKTGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAIELIGEKSFIQMPEEE 141

Query: 76  HKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIF 135
           HKRLRRLTSAPVNGFEALSNYIPYIE+NVL+SL+KWS MGPIEFLTQLRKLTFT+IMYIF
Sbjct: 142 HKRLRRLTSAPVNGFEALSNYIPYIEKNVLESLEKWSKMGPIEFLTQLRKLTFTVIMYIF 201

Query: 136 LSAESESVMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRR 195
           LS+ESE VME LEKEYTRLNYGVRA+RIN+PGFAYHKALKARKNLVAA +GIVTERR+++
Sbjct: 202 LSSESEPVMEMLEKEYTRLNYGVRAMRINLPGFAYHKALKARKNLVAALKGIVTERRRQK 261

Query: 196 LGNWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQH 255
           L  WAPKRKDMMD LID+EDENGRKL DEEIIDIL+MYLNAGHESSGHTMMWATILLNQH
Sbjct: 262 LDKWAPKRKDMMDQLIDIEDENGRKLDDEEIIDILIMYLNAGHESSGHTMMWATILLNQH 321

Query: 256 PEVLKKAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMD 315
           PEVLKKA+EEQE IVR RPAGQ GLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMD
Sbjct: 322 PEVLKKAREEQEAIVRNRPAGQTGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMD 381

Query: 316 VNLNGYLIPKGWKVLAWFRSIHYDNEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCP 375
           VNLNGYLIPKGWKVLAWFRSIHYD+EVYPDPKKFDPSRWDGF+PKAGEFLPFGAGSRLCP
Sbjct: 382 VNLNGYLIPKGWKVLAWFRSIHYDSEVYPDPKKFDPSRWDGFVPKAGEFLPFGAGSRLCP 441

Query: 376 GNDLAKLEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISKNSS 428
           GNDLAKLEICIF+HYFLLNY LEWLTPDC+ILYLPHSRPKDNC+AKI+K SS
Sbjct: 442 GNDLAKLEICIFVHYFLLNYNLEWLTPDCEILYLPHSRPKDNCMAKITKKSS 493

[Close]
BLAST of Chy6G108150 vs. ExPASy TrEMBL
Match: A0A6J1IVT9 (ent-kaurenoic acid oxidase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111479695 PE=3 SV=1)

HSP 1 Score: 785.0 bits (2026), Expect = 1.6e-223
Identity = 371/412 (90.05%), Postives = 396/412 (96.12%), Query Frame = 0 

Query: 16  RFGKIGVYKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEE 75
           R+GK GVYK+HLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIG+KSFI+MP EE
Sbjct: 82  RYGKTGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGEKSFIQMPQEE 141

Query: 76  HKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIF 135
           HKRLRRLTSAPVNGFEALSNYIPYIE+NVL+SL+KWS MGPIEFLTQLRKLTFT+IMYIF
Sbjct: 142 HKRLRRLTSAPVNGFEALSNYIPYIEKNVLESLEKWSKMGPIEFLTQLRKLTFTVIMYIF 201

Query: 136 LSAESESVMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRR 195
           LS+ESE VME LEKEYTRLNYGVRA+RIN+PGFAYHKALKARKNLVAA +GIVTERR+++
Sbjct: 202 LSSESEPVMEMLEKEYTRLNYGVRAMRINLPGFAYHKALKARKNLVAALKGIVTERRRQK 261

Query: 196 LGNWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQH 255
           L  WAPKRKDMMD LID+ DENGRKL DEEIIDIL+MYLNAGHESSGHTMMWATILLNQH
Sbjct: 262 LDKWAPKRKDMMDQLIDIVDENGRKLDDEEIIDILIMYLNAGHESSGHTMMWATILLNQH 321

Query: 256 PEVLKKAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMD 315
           PEVLKKA+EEQE IVR RPAGQ GLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMD
Sbjct: 322 PEVLKKAREEQEAIVRNRPAGQTGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMD 381

Query: 316 VNLNGYLIPKGWKVLAWFRSIHYDNEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCP 375
           VNLNGYLIPKGWKVLAWFRSIHYD+EVYPDPKKF+PSRWDGF+PKAGEFLPFGAGSRLCP
Sbjct: 382 VNLNGYLIPKGWKVLAWFRSIHYDSEVYPDPKKFEPSRWDGFVPKAGEFLPFGAGSRLCP 441

Query: 376 GNDLAKLEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISKNSS 428
           GNDLAKLEICIF+HYFLLNY LEWLTPDC+ILYLPHSRPKDNC+AKI+K SS
Sbjct: 442 GNDLAKLEICIFVHYFLLNYNLEWLTPDCEILYLPHSRPKDNCMAKITKKSS 493

[Close]
BLAST of Chy6G108150 vs. NCBI nr
Match: XP_004133946.1 (ent-kaurenoic acid oxidase 1 isoform X1 [Cucumis sativus] >KAE8650281.1 hypothetical protein Csa_010193 [Cucumis sativus])

HSP 1 Score: 845 bits (2184), Expect = 5.99e-308
Identity = 414/415 (99.76%), Postives = 415/415 (100.00%), Query Frame = 0 

Query: 16  RFGKIGVYKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEE 75
           RFGKIGVYKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEE
Sbjct: 84  RFGKIGVYKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEE 143

Query: 76  HKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIF 135
           HKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIF
Sbjct: 144 HKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIF 203

Query: 136 LSAESESVMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRR 195
           LSAESESVMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRR
Sbjct: 204 LSAESESVMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRR 263

Query: 196 LGNWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQH 255
           LGNWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQH
Sbjct: 264 LGNWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQH 323

Query: 256 PEVLKKAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMD 315
           PEVLKKAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMD
Sbjct: 324 PEVLKKAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMD 383

Query: 316 VNLNGYLIPKGWKVLAWFRSIHYDNEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCP 375
           VNLNGYLIPKGWKVLAWFRSIHYD+EVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCP
Sbjct: 384 VNLNGYLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCP 443

Query: 376 GNDLAKLEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISKNSSTVV 430
           GNDLAKLEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISKNSSTVV
Sbjct: 444 GNDLAKLEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISKNSSTVV 498

[Close]
BLAST of Chy6G108150 vs. NCBI nr
Match: KAA0049080.1 (ent-kaurenoic acid oxidase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 830 bits (2144), Expect = 1.32e-302
Identity = 405/415 (97.59%), Postives = 409/415 (98.55%), Query Frame = 0 

Query: 16  RFGKIGVYKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEE 75
           RFGKIGVYK+HLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEE
Sbjct: 38  RFGKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEE 97

Query: 76  HKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIF 135
           HKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWS MGPIEFLTQLRKLTFTIIMYIF
Sbjct: 98  HKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSTMGPIEFLTQLRKLTFTIIMYIF 157

Query: 136 LSAESESVMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRR 195
           LSAESESVMESLEKEYTRLNYGVRALRIN+PGFAYHKALKARKNLVAA QGIVTERRKRR
Sbjct: 158 LSAESESVMESLEKEYTRLNYGVRALRINLPGFAYHKALKARKNLVAALQGIVTERRKRR 217

Query: 196 LGNWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQH 255
           LG WAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQH
Sbjct: 218 LGKWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQH 277

Query: 256 PEVLKKAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMD 315
           PEVLKKAKEEQEEIVRRRP GQKGLTLKECR+MEYLSKVVDETLRYVSFSLV FREAQMD
Sbjct: 278 PEVLKKAKEEQEEIVRRRPPGQKGLTLKECREMEYLSKVVDETLRYVSFSLVAFREAQMD 337

Query: 316 VNLNGYLIPKGWKVLAWFRSIHYDNEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCP 375
           VNLNGYLIPKGWKVLAWFRSIHYD+EVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCP
Sbjct: 338 VNLNGYLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCP 397

Query: 376 GNDLAKLEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISKNSSTVV 430
           GNDLAKLEICIFIHYFLLNYKLEWLTPDC ILYLPHSRPKDNCLAKISKNSSTVV
Sbjct: 398 GNDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSRPKDNCLAKISKNSSTVV 452

[Close]
BLAST of Chy6G108150 vs. NCBI nr
Match: TYK17483.1 (ent-kaurenoic acid oxidase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 832 bits (2148), Expect = 1.83e-302
Identity = 406/415 (97.83%), Postives = 410/415 (98.80%), Query Frame = 0 

Query: 16  RFGKIGVYKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEE 75
           RFGKIGVYK+HLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEE
Sbjct: 84  RFGKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEE 143

Query: 76  HKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIF 135
           HKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWS MGPIEFLTQLRKLTFTIIMYIF
Sbjct: 144 HKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSTMGPIEFLTQLRKLTFTIIMYIF 203

Query: 136 LSAESESVMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRR 195
           LSAESESVMESLEKEYTRLNYGVRALRIN+PGFAYHKALKARKNLVAA QGIVTERRKRR
Sbjct: 204 LSAESESVMESLEKEYTRLNYGVRALRINLPGFAYHKALKARKNLVAALQGIVTERRKRR 263

Query: 196 LGNWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQH 255
           LG WAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQH
Sbjct: 264 LGKWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQH 323

Query: 256 PEVLKKAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMD 315
           PEVLKKAKEEQEEIVRRRP GQKGLTLKECR+MEYLSKVVDETLRYVSFSLVVFREAQMD
Sbjct: 324 PEVLKKAKEEQEEIVRRRPPGQKGLTLKECREMEYLSKVVDETLRYVSFSLVVFREAQMD 383

Query: 316 VNLNGYLIPKGWKVLAWFRSIHYDNEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCP 375
           VNLNGYLIPKGWKVLAWFRSIHYD+EVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCP
Sbjct: 384 VNLNGYLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCP 443

Query: 376 GNDLAKLEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISKNSSTVV 430
           GNDLAKLEICIFIHYFLLNYKLEWLTPDC ILYLPHSRPKDNCLAKISKNSSTVV
Sbjct: 444 GNDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSRPKDNCLAKISKNSSTVV 498

[Close]
BLAST of Chy6G108150 vs. NCBI nr
Match: XP_008438234.1 (PREDICTED: ent-kaurenoic acid oxidase 2-like [Cucumis melo])

HSP 1 Score: 830 bits (2144), Expect = 7.44e-302
Identity = 405/415 (97.59%), Postives = 409/415 (98.55%), Query Frame = 0 

Query: 16  RFGKIGVYKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEE 75
           RFGKIGVYK+HLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEE
Sbjct: 84  RFGKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEE 143

Query: 76  HKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIF 135
           HKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWS MGPIEFLTQLRKLTFTIIMYIF
Sbjct: 144 HKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSTMGPIEFLTQLRKLTFTIIMYIF 203

Query: 136 LSAESESVMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRR 195
           LSAESESVMESLEKEYTRLNYGVRALRIN+PGFAYHKALKARKNLVAA QGIVTERRKRR
Sbjct: 204 LSAESESVMESLEKEYTRLNYGVRALRINLPGFAYHKALKARKNLVAALQGIVTERRKRR 263

Query: 196 LGNWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQH 255
           LG WAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQH
Sbjct: 264 LGKWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQH 323

Query: 256 PEVLKKAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMD 315
           PEVLKKAKEEQEEIVRRRP GQKGLTLKECR+MEYLSKVVDETLRYVSFSLVVFREAQMD
Sbjct: 324 PEVLKKAKEEQEEIVRRRPPGQKGLTLKECREMEYLSKVVDETLRYVSFSLVVFREAQMD 383

Query: 316 VNLNGYLIPKGWKVLAWFRSIHYDNEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCP 375
           VNLNGYLIPKGWKVLAWFRSIHYD+EVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCP
Sbjct: 384 VNLNGYLIPKGWKVLAWFRSIHYDDEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCP 443

Query: 376 GNDLAKLEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISKNSSTVV 430
           GNDLAKLEICIFIHYFLLNYKLEWLTPDC ILYLPHSRPKDNCLAKISKNSSTV 
Sbjct: 444 GNDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSRPKDNCLAKISKNSSTVA 498

[Close]
BLAST of Chy6G108150 vs. NCBI nr
Match: XP_038902869.1 (ent-kaurenoic acid oxidase 1-like [Benincasa hispida])

HSP 1 Score: 810 bits (2092), Expect = 4.96e-294
Identity = 394/415 (94.94%), Postives = 404/415 (97.35%), Query Frame = 0 

Query: 16  RFGKIGVYKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEE 75
           RFGKIGVYK+HLFGNPSVVVTTPETCRKVLTDDEAFQPGWPR+A+ELIGKKSFIEMPVEE
Sbjct: 78  RFGKIGVYKVHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRSAIELIGKKSFIEMPVEE 137

Query: 76  HKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIF 135
           HKRLRRLTSAPVNGFEALSNYIPYIEE+VLKSL KWS+MGPIEFLTQLRKLTFTIIMYIF
Sbjct: 138 HKRLRRLTSAPVNGFEALSNYIPYIEESVLKSLGKWSDMGPIEFLTQLRKLTFTIIMYIF 197

Query: 136 LSAESESVMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRR 195
           LSAESE VME+LEKEYTRLNYGVRALRIN PGFAYHKALKARKNLVAA Q IVT+RRKRR
Sbjct: 198 LSAESEPVMEALEKEYTRLNYGVRALRINFPGFAYHKALKARKNLVAALQRIVTDRRKRR 257

Query: 196 LGNWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQH 255
           L  WAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQH
Sbjct: 258 LDKWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQH 317

Query: 256 PEVLKKAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMD 315
           PEVLKKAK EQEEIVRRRP GQKGLTLKECR+MEYLSKVVDETLRYVSFSLVVFREAQMD
Sbjct: 318 PEVLKKAKAEQEEIVRRRPPGQKGLTLKECREMEYLSKVVDETLRYVSFSLVVFREAQMD 377

Query: 316 VNLNGYLIPKGWKVLAWFRSIHYDNEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCP 375
           VNLNGYLIPKGWKVLAWFRSIHYD+EVYPDPKKFDPSRWD F+PKAGEFLPFGAGSRLCP
Sbjct: 378 VNLNGYLIPKGWKVLAWFRSIHYDSEVYPDPKKFDPSRWDNFVPKAGEFLPFGAGSRLCP 437

Query: 376 GNDLAKLEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISKNSSTVV 430
           GNDLAKLEICIFIHYFLLNYKLEWLTPDC ILYLPHSRPKDNCLAKISKNSSTVV
Sbjct: 438 GNDLAKLEICIFIHYFLLNYKLEWLTPDCPILYLPHSRPKDNCLAKISKNSSTVV 492

[Close]
BLAST of Chy6G108150 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 622.1 bits (1603), Expect = 3.4e-178
Identity = 286/409 (69.93%), Postives = 350/409 (85.57%), Query Frame = 0 

Query: 16  RFGKIGVYKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEE 75
           R+G  G+YK H+FGNPS++VTT +TCR+VLTDD+AF+PGWP + +ELIG+KSF+ +  EE
Sbjct: 80  RYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEE 139

Query: 76  HKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIF 135
           HKRLRRLT+APVNG EALS YIPYIEENV+  LDKW+ MG  EFLT LRKLTF IIMYIF
Sbjct: 140 HKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGEFEFLTHLRKLTFRIIMYIF 199

Query: 136 LSAESESVMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRR 195
           LS+ESE+VM++LE+EYT LNYGVRA+ +NIPGFAYH+ALKARK LVAAFQ IVTERR +R
Sbjct: 200 LSSESENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAFQSIVTERRNQR 259

Query: 196 LGNWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQH 255
             N    +KDM+D L++V+DE+G+ L DEEIID+L+MYLNAGHESSGHT+MWAT+ L +H
Sbjct: 260 KQNILSNKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQEH 319

Query: 256 PEVLKKAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMD 315
           PEVL++AK EQE I++ RP GQKGL+LKE R ME+LS+VVDETLR ++FSL  FREA+ D
Sbjct: 320 PEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVITFSLTAFREAKTD 379

Query: 316 VNLNGYLIPKGWKVLAWFRSIHYDNEVYPDPKKFDPSRWD-GFIPKAGEFLPFGAGSRLC 375
           V +NGYLIPKGWKVL WFR +H D EV+PDP+KFDP+RWD GF+PKAG FLPFGAGS LC
Sbjct: 380 VEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGAFLPFGAGSHLC 439

Query: 376 PGNDLAKLEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKIS 424
           PGNDLAKLEI IF+H+FLL Y+++   P+C ++YLPH+RP DNCLA+IS
Sbjct: 440 PGNDLAKLEISIFLHHFLLKYQVKRSNPECPVMYLPHTRPTDNCLARIS 488

[Close]
BLAST of Chy6G108150 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 606.3 bits (1562), Expect = 1.9e-173
Identity = 283/409 (69.19%), Postives = 344/409 (84.11%), Query Frame = 0 

Query: 16  RFGKIGVYKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEE 75
           R+G+ G+YK H+FG P V+VTTPETCR+VLTDD+AF  GWP++ ++LIG+KSF+ +  EE
Sbjct: 79  RYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSFVGISFEE 138

Query: 76  HKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIF 135
           HKRLRRLTSAPVNG EALS YI +IEE V   L+KWS MG IEFL+ LRKLTF +IMYIF
Sbjct: 139 HKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGEIEFLSHLRKLTFKVIMYIF 198

Query: 136 LSAESESVMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRR 195
           LS+ESE VM+SLE+EYT LNYGVRA+ IN+PGFAYH+ALKARK LVAAFQ IVT RR +R
Sbjct: 199 LSSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAFQSIVTNRRNQR 258

Query: 196 LGNWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQH 255
             N +  RKDM+D LIDV+DENGR L DEEIID+L+MYLNAGHESSGH  MWATIL+ +H
Sbjct: 259 KQNISSNRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGHLTMWATILMQEH 318

Query: 256 PEVLKKAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMD 315
           P +L+KAKEEQE IV++R  GQK LTLKE R+M YLS+V+DETLR ++FSL  FREA+ D
Sbjct: 319 PMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVITFSLTAFREAKSD 378

Query: 316 VNLNGYLIPKGWKVLAWFRSIHYDNEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCP 375
           V ++GY+IPKGWKVL WFR++H D E+YPDPKKFDPSRW+G+ PKAG FLPFG GS LCP
Sbjct: 379 VQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTFLPFGLGSHLCP 438

Query: 376 GNDLAKLEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISK 425
           GNDLAKLEI IF+H+FLL Y++E   P C +++LPH+RPKDNCLA+I++
Sbjct: 439 GNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARITR 486

[Close]
BLAST of Chy6G108150 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 606.3 bits (1562), Expect = 1.9e-173
Identity = 283/409 (69.19%), Postives = 344/409 (84.11%), Query Frame = 0 

Query: 16  RFGKIGVYKIHLFGNPSVVVTTPETCRKVLTDDEAFQPGWPRAAVELIGKKSFIEMPVEE 75
           R+G+ G+YK H+FG P V+VTTPETCR+VLTDD+AF  GWP++ ++LIG+KSF+ +  EE
Sbjct: 79  RYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSFVGISFEE 138

Query: 76  HKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIMYIF 135
           HKRLRRLTSAPVNG EALS YI +IEE V   L+KWS MG IEFL+ LRKLTF +IMYIF
Sbjct: 139 HKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGEIEFLSHLRKLTFKVIMYIF 198

Query: 136 LSAESESVMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRKRR 195
           LS+ESE VM+SLE+EYT LNYGVRA+ IN+PGFAYH+ALKARK LVAAFQ IVT RR +R
Sbjct: 199 LSSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAFQSIVTNRRNQR 258

Query: 196 LGNWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLNQH 255
             N +  RKDM+D LIDV+DENGR L DEEIID+L+MYLNAGHESSGH  MWATIL+ +H
Sbjct: 259 KQNISSNRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGHLTMWATILMQEH 318

Query: 256 PEVLKKAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQMD 315
           P +L+KAKEEQE IV++R  GQK LTLKE R+M YLS+V+DETLR ++FSL  FREA+ D
Sbjct: 319 PMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVITFSLTAFREAKSD 378

Query: 316 VNLNGYLIPKGWKVLAWFRSIHYDNEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRLCP 375
           V ++GY+IPKGWKVL WFR++H D E+YPDPKKFDPSRW+G+ PKAG FLPFG GS LCP
Sbjct: 379 VQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTFLPFGLGSHLCP 438

Query: 376 GNDLAKLEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISK 425
           GNDLAKLEI IF+H+FLL Y++E   P C +++LPH+RPKDNCLA+I++
Sbjct: 439 GNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARITR 486

[Close]
BLAST of Chy6G108150 vs. TAIR 10
Match: AT3G19270.1 (cytochrome P450, family 707, subfamily A, polypeptide 4 )

HSP 1 Score: 248.4 bits (633), Expect = 1.0e-65
Identity = 145/413 (35.11%), Postives = 229/413 (55.45%), Query Frame = 0 

Query: 16  RFGKIGVYKIHLFGNPSVVVTTPETCRKVL-TDDEAFQPGWPRAAVELIGKKSFIEMPVE 75
           R+G+I  +K  + G P V++ +PE  R VL T    F+P +PR+  +LIG  +      +
Sbjct: 65  RYGEI--FKTRILGYPCVMLASPEAARFVLVTHAHMFKPTYPRSKEKLIGPSALFFHQGD 124

Query: 76  EHKRLRRLTSAPVNGFEALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTI-IMY 135
            H  +R+L  +     E +   IP IE   L SL  W+NM  +    +++K  F + I+ 
Sbjct: 125 YHSHIRKLVQSSFYP-ETIRKLIPDIEHIALSSLQSWANMPIVSTYQEMKKFAFDVGILA 184

Query: 136 IFLSAESESVMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERRK 195
           IF   ES S  E L+  Y  ++ G  +  +++PG +YHKAL ARK L      I+ ERR+
Sbjct: 185 IFGHLES-SYKEILKHNYNIVDKGYNSFPMSLPGTSYHKALMARKQLKTIVSEIICERRE 244

Query: 196 RRLGNWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILLN 255
           +R       + D +  L++ ++E GR LT E+I D ++  L A  +++   + W    L+
Sbjct: 245 KR-----ALQTDFLGHLLNFKNEKGRVLTQEQIADNIIGVLFAAQDTTASCLTWILKYLH 304

Query: 256 QHPEVLKKAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREAQ 315
              ++L+  K EQ+ I       +K LT ++ R+M    KV+ E+LR  S     FREA 
Sbjct: 305 DDQKLLEAVKAEQKAIYEENSREKKPLTWRQTRNMPLTHKVIVESLRMASIISFTFREAV 364

Query: 316 MDVNLNGYLIPKGWKVLAWFRSIHYDNEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSRL 375
           +DV   GYLIPKGWKV+  FR+IH++ + + +P+ FDPSR++   PK   F+PFG+G   
Sbjct: 365 VDVEYKGYLIPKGWKVMPLFRNIHHNPKYFSNPEVFDPSRFE-VNPKPNTFMPFGSGVHA 424

Query: 376 CPGNDLAKLEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKDNCLAKISKNS 427
           CPGN+LAKL+I IF+H+ + N++ E    +  I Y P   P++   A   ++S
Sbjct: 425 CPGNELAKLQILIFLHHLVSNFRWEVKGGEKGIQYSPFPIPQNGLPATFRRHS 467

[Close]
BLAST of Chy6G108150 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 237.3 bits (604), Expect = 2.3e-62
Identity = 137/404 (33.91%), Postives = 222/404 (54.95%), Query Frame = 0 

Query: 16  RFGKIGVYKIHLFGNPSVVVTTPETCRKVL-TDDEAFQPGWPRAAVELIGKKSFIEMPVE 75
           R+G   V+K H+ G P V++++PE  + VL T    F+P +P +   ++GK++      +
Sbjct: 67  RYG--SVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGD 126

Query: 76  EHKRLRRLTSAPVNGF--EALSNYIPYIEENVLKSLDKWSNMGPIEFLTQLRKLTFTIIM 135
            H +LR+L    +  F  +A+ N +P+IE    +SL+ W     +    +++  TF + +
Sbjct: 127 YHSKLRKLV---LRAFMPDAIRNMVPHIESIAQESLNSWDGT-QLNTYQEMKTYTFNVAL 186

Query: 136 YIFLSAESESVMESLEKEYTRLNYGVRALRINIPGFAYHKALKARKNLVAAFQGIVTERR 195
              L  +     E L++ Y  L  G  ++ IN+PG  +HKA+KARK L      I+++RR
Sbjct: 187 ISILGKDEVYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILSKRR 246

Query: 196 KRRLGNWAPKRKDMMDALIDVEDENGRKLTDEEIIDILVMYLNAGHESSGHTMMWATILL 255
           +           D++ +   +ED+ G  LTDE+I D ++  + A  +++   + W    L
Sbjct: 247 QN-----PSSHTDLLGSF--MEDKAG--LTDEQIADNIIGVIFAARDTTASVLTWILKYL 306

Query: 256 NQHPEVLKKAKEEQEEIVRRRPAGQKGLTLKECRDMEYLSKVVDETLRYVSFSLVVFREA 315
             +P VL+   EEQ  I + +  G+  LT ++ + M    +V+ ETLR  +     FREA
Sbjct: 307 ADNPTVLEAVTEEQMAIRKDKKEGE-SLTWEDTKKMPLTYRVIQETLRAATILSFTFREA 366

Query: 316 QMDVNLNGYLIPKGWKVLAWFRSIHYDNEVYPDPKKFDPSRWDGFIPKAGEFLPFGAGSR 375
             DV   GYLIPKGWKVL  FR+IH++ +++ DP KFDPSR++   PK   F+PFG+G  
Sbjct: 367 VEDVEYEGYLIPKGWKVLPLFRNIHHNADIFSDPGKFDPSRFE-VAPKPNTFMPFGSGIH 426

Query: 376 LCPGNDLAKLEICIFIHYFLLNYKLEWLTPDCQILYLPHSRPKD 417
            CPGN+LAKLEI + IH+    Y+   + P   I Y P + P++
Sbjct: 427 SCPGNELAKLEISVLIHHLTTKYRWSIVGPSDGIQYGPFALPQN 453

The following BLAST results are available for this feature:
BLAST of Chy6G108150 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O230514.8e-17769.93Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1[more]
Q9C5Y22.7e-17269.19Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2[more]
Q9AXH95.5e-14959.33Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1[more]
Q432465.7e-14657.63Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1[more]
Q5VRM72.8e-14556.76Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ... [more]
BLAST of Chy6G108150 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5D3CZZ04.5e-23997.83Ent-kaurenoic acid oxidase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A5A7U1001.3e-23897.59Ent-kaurenoic acid oxidase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
A0A1S3AWI41.3e-23897.83ent-kaurenoic acid oxidase 2-like OS=Cucumis melo OX=3656 GN=LOC103483403 PE=3 S... [more]
A0A6J1E9S41.1e-22490.29ent-kaurenoic acid oxidase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111432076 ... [more]
A0A6J1IVT91.6e-22390.05ent-kaurenoic acid oxidase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111479695 PE... [more]
BLAST of Chy6G108150 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004133946.15.99e-30899.76ent-kaurenoic acid oxidase 1 isoform X1 [Cucumis sativus] >KAE8650281.1 hypothet... [more]
KAA0049080.11.32e-30297.59ent-kaurenoic acid oxidase 2-like [Cucumis melo var. makuwa][more]
TYK17483.11.83e-30297.83ent-kaurenoic acid oxidase 2-like [Cucumis melo var. makuwa][more]
XP_008438234.17.44e-30297.59PREDICTED: ent-kaurenoic acid oxidase 2-like [Cucumis melo][more]
XP_038902869.14.96e-29494.94ent-kaurenoic acid oxidase 1-like [Benincasa hispida][more]
BLAST of Chy6G108150 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G05160.13.4e-17869.93cytochrome P450, family 88, subfamily A, polypeptide 3 [more]
AT2G32440.11.9e-17369.19ent-kaurenoic acid hydroxylase 2 [more]
AT2G32440.21.9e-17369.19ent-kaurenoic acid hydroxylase 2 [more]
AT3G19270.11.0e-6535.11cytochrome P450, family 707, subfamily A, polypeptide 4 [more]
AT5G45340.12.3e-6233.91cytochrome P450, family 707, subfamily A, polypeptide 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 245..271
score: 28.82
coord: 374..397
score: 29.77
coord: 364..374
score: 57.74
coord: 332..356
score: 26.05
IPR001128Cytochrome P450PRINTSPR00385P450coord: 365..374
score: 59.89
coord: 236..253
score: 27.64
coord: 293..304
score: 33.11
coord: 374..385
score: 38.98
IPR001128Cytochrome P450PFAMPF00067p450coord: 22..399
e-value: 1.3E-64
score: 218.6
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 7..425
e-value: 1.5E-100
score: 339.1
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 18..422
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 14..418
NoneNo IPR availablePANTHERPTHR24286:SF289ENT-KAURENOIC ACID OXIDASE 2coord: 14..418
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 367..376

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy6G108150.1Chy6G108150.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen