Chy6G108060 (gene) Cucumber (hystrix) v1

Overview
NameChy6G108060
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionCyclin
LocationchrH06: 1864289 .. 1865528 (+)
RNA-Seq ExpressionChy6G108060
SyntenyChy6G108060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGAGCTAGAGGATTCAACTGTCATGGGGAAGCTAATCGATTTCCTTTCTTGTCTTCTTCAACGAGTGGCCGAATCCAACGATCGAAACCTCTCTGTTAATCTTCAACCTCACAAAATCTCTGCCTTCCATGGCTTAACTCGCCCCTCCATTTCCATCCAAAGCTACTTAGACAGAATCTTTAAGTACGCCAATTGTAGCCCTTGTTGCTTCGTCATTGCCTACGTTTATCTCGATCGCTTTGTTCAAAGACAACCCTCTTTGCCTATCAATTCCTTCAATGTTCATCGCTTGCTTATCACTAGCGTTCTTGTTTCTGCCAAATTTATGGATGATACGTGAGTTTTTCTAATCTGATTGACAGAATTTTCGATGTGGGCTTTGATTTGAGCCTTTTTTTTTAGTTTGCCTGCTAAGTTCCAGATGAAATGCCTCAATGAGTTCTGTTTTTGAGTTTTTTTTTAAACTGTGAGATAAGGGGTTTTAAGAGAAAGTTTTGGGTTTTCTATTATGGGGTTTTTGGGTATAATTGGGATTAGAGATTGAATGGAACTTGATGTTTTGCAAAAGATTGTTTTATTTTCATTTTCAAGGATCTTTTTTATGTGAGTTTGTTTTGATGAATGTGATGCTTTGAGCAAATTCTTGTTTATTCTTGATCTCTTTCTTTGGTTGGTTTTCTGGGAATCTTTTTCTCCTTGTGCATATCTTTTCATCACATTATTTGCTGAATATGCTTCTCTCTCTCATTTTCAACAGCTAGGCAGCCAGCTAAAAAGCATAATGAAACAATCATGAATTTTACTTTAGATAATACAAAAAGGCTGCAAATTTTGGTTCCATCATCATCCTTTTTTCTTAAAATATTCAAAAGGGGGCTTCGATGTTTAGGCAATTTGAGTTAAAGTTTACTGAAATATGCAATGATGAAATTGTTTTCAGGTATTACAACAATGCATATTATGCAAAAGTAGGAGGGATCAGCACAACAGAAATGAACTTTCTTGAAGTGGATTTTCTGTTTGGTTTAGGCTTTCATTTGAACGTCACTCCCACCACTTTCCATTCTTATTACTCATATCTTCAAAGACAAATGCTTCTGCTTCAGCCTCCTTTGACGAGCACTGCTTCTACAAAATCAGATCTGCTGCTTAGTTCATCAAGAGCTCTAAAATCCCATTTCTGTTTTGATGAAGATGAAGCTTCCCATAAGAAGCAACAACTTGCAGCTGTTTGA

mRNA sequence

ATGGCGGAGCTAGAGGATTCAACTGTCATGGGGAAGCTAATCGATTTCCTTTCTTGTCTTCTTCAACGAGTGGCCGAATCCAACGATCGAAACCTCTCTGTTAATCTTCAACCTCACAAAATCTCTGCCTTCCATGGCTTAACTCGCCCCTCCATTTCCATCCAAAGCTACTTAGACAGAATCTTTAAGTACGCCAATTGTAGCCCTTGTTGCTTCGTCATTGCCTACGTTTATCTCGATCGCTTTGTTCAAAGACAACCCTCTTTGCCTATCAATTCCTTCAATGTTCATCGCTTGCTTATCACTAGCGTTCTTGTTTCTGCCAAATTTATGGATGATACGTATTACAACAATGCATATTATGCAAAAGTAGGAGGGATCAGCACAACAGAAATGAACTTTCTTGAAGTGGATTTTCTGTTTGGTTTAGGCTTTCATTTGAACGTCACTCCCACCACTTTCCATTCTTATTACTCATATCTTCAAAGACAAATGCTTCTGCTTCAGCCTCCTTTGACGAGCACTGCTTCTACAAAATCAGATCTGCTGCTTAGTTCATCAAGAGCTCTAAAATCCCATTTCTGTTTTGATGAAGATGAAGCTTCCCATAAGAAGCAACAACTTGCAGCTGTTTGA

Coding sequence (CDS)

ATGGCGGAGCTAGAGGATTCAACTGTCATGGGGAAGCTAATCGATTTCCTTTCTTGTCTTCTTCAACGAGTGGCCGAATCCAACGATCGAAACCTCTCTGTTAATCTTCAACCTCACAAAATCTCTGCCTTCCATGGCTTAACTCGCCCCTCCATTTCCATCCAAAGCTACTTAGACAGAATCTTTAAGTACGCCAATTGTAGCCCTTGTTGCTTCGTCATTGCCTACGTTTATCTCGATCGCTTTGTTCAAAGACAACCCTCTTTGCCTATCAATTCCTTCAATGTTCATCGCTTGCTTATCACTAGCGTTCTTGTTTCTGCCAAATTTATGGATGATACGTATTACAACAATGCATATTATGCAAAAGTAGGAGGGATCAGCACAACAGAAATGAACTTTCTTGAAGTGGATTTTCTGTTTGGTTTAGGCTTTCATTTGAACGTCACTCCCACCACTTTCCATTCTTATTACTCATATCTTCAAAGACAAATGCTTCTGCTTCAGCCTCCTTTGACGAGCACTGCTTCTACAAAATCAGATCTGCTGCTTAGTTCATCAAGAGCTCTAAAATCCCATTTCTGTTTTGATGAAGATGAAGCTTCCCATAAGAAGCAACAACTTGCAGCTGTTTGA

Protein sequence

MAELEDSTVMGKLIDFLSCLLQRVAESNDRNLSVNLQPHKISAFHGLTRPSISIQSYLDRIFKYANCSPCCFVIAYVYLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAYYAKVGGISTTEMNFLEVDFLFGLGFHLNVTPTTFHSYYSYLQRQMLLLQPPLTSTASTKSDLLLSSSRALKSHFCFDEDEASHKKQQLAAV*
Homology
[Close]
BLAST of Chy6G108060 vs. ExPASy Swiss-Prot
Match: O80513 (Cyclin-U4-1 OS=Arabidopsis thaliana OX=3702 GN=CYCU4-1 PE=1 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 5.7e-75
Identity = 144/207 (69.57%), Postives = 170/207 (82.13%), Query Frame = 0 

Query: 1   MAELEDSTVMGKLIDFLSCLLQRVAESNDRNLSVNLQPHKISAFHGLTRPSISIQSYLDR 60
           MAELE+ +VM KLI FLS LL+RVAESND    V  Q  ++S FHGL+RP+I+IQSYL+R
Sbjct: 1   MAELENPSVMSKLIAFLSSLLERVAESNDLTRRVATQSQRVSVFHGLSRPTITIQSYLER 60

Query: 61  IFKYANCSPCCFVIAYVYLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAY 120
           IFKYANCSP CFV+AYVYLDRF  RQPSLPINSFNVHRLLITSV+V+AKF+DD YYNNAY
Sbjct: 61  IFKYANCSPSCFVVAYVYLDRFTHRQPSLPINSFNVHRLLITSVMVAAKFLDDLYYNNAY 120

Query: 121 YAKVGGISTTEMNFLEVDFLFGLGFHLNVTPTTFHSYYSYLQRQMLLLQPPLTSTASTKS 180
           YAKVGGIST EMNFLE+DFLFGLGF LNVTP TF++Y+SYLQ++M LLQP         S
Sbjct: 121 YAKVGGISTKEMNFLELDFLFGLGFELNVTPNTFNAYFSYLQKEMTLLQP--------LS 180

Query: 181 DLLLSSSRALKSHFCFDEDEASHKKQQ 208
            +++ SSR+L     F++DEASH+KQQ
Sbjct: 181 LVVVPSSRSL---ITFNDDEASHQKQQ 196

[Close]
BLAST of Chy6G108060 vs. ExPASy Swiss-Prot
Match: Q9FKF6 (Cyclin-U4-3 OS=Arabidopsis thaliana OX=3702 GN=CYCU4-3 PE=1 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 2.3e-55
Identity = 120/204 (58.82%), Postives = 152/204 (74.51%), Query Frame = 0 

Query: 9   VMGKLIDFLSCLLQRVAESNDRNLSVNLQPHKISAFHGLTRPSISIQSYLDRIFKYANCS 68
           +M  ++  +S LLQRV+E+ND NLS   Q  K S+F G+T+PSISI+SYL+RIF+YANCS
Sbjct: 21  IMPSVLTAMSYLLQRVSETND-NLS---QKQKPSSFTGVTKPSISIRSYLERIFEYANCS 80

Query: 69  PCCFVIAYVYLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAYYAKVGGIS 128
             C+++AY+YLDRFV++QP LPINSFNVHRL+ITSVLVSAKFMDD  YNN YYAKVGGIS
Sbjct: 81  YSCYIVAYIYLDRFVKKQPFLPINSFNVHRLIITSVLVSAKFMDDLSYNNEYYAKVGGIS 140

Query: 129 TTEMNFLEVDFLFGLGFHLNVTPTTFHSYYSYLQRQMLLLQPPLTSTASTKSDLLLSSSR 188
             EMN LE+DFLFG+GF LNVT +TF++Y  +LQR+M +L   + S     S   +SS  
Sbjct: 141 REEMNMLELDFLFGIGFELNVTVSTFNNYCCFLQREMAMLM-KMKSLFLEPSSFKISSKT 200

Query: 189 ALKSHFCFDEDEAS--HKKQQLAA 211
            L   +  +ED  S  H K+QLAA
Sbjct: 201 KLVM-YPHEEDSLSTHHNKKQLAA 218

[Close]
BLAST of Chy6G108060 vs. ExPASy Swiss-Prot
Match: Q9LY16 (Cyclin-U4-2 OS=Arabidopsis thaliana OX=3702 GN=CYCU4-2 PE=1 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 1.4e-52
Identity = 114/205 (55.61%), Postives = 154/205 (75.12%), Query Frame = 0 

Query: 9   VMGKLIDFLSCLLQRVAESNDRNLSVNLQPHK-ISAFHGLTRPSISIQSYLDRIFKYANC 68
           +M  +I  +S LLQRV+E+ND +LS   + HK ISAF+ +T+PSISI+SY++RIFKYA+C
Sbjct: 19  IMPNVITAMSSLLQRVSETND-DLSRPFREHKRISAFNAVTKPSISIRSYMERIFKYADC 78

Query: 69  SPCCFVIAYVYLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAYYAKVGGI 128
           S  C+++AY+YLDRF+Q+QP LPI+S NVHRL+ITSVLVSAKFMDD  YNNA+YAKVGGI
Sbjct: 79  SDSCYIVAYIYLDRFIQKQPLLPIDSSNVHRLIITSVLVSAKFMDDLCYNNAFYAKVGGI 138

Query: 129 STTEMNFLEVDFLFGLGFHLNVTPTTFHSYYSYLQRQMLL--LQPPLTSTASTKSDLLLS 188
           +T EMN LE+DFLFG+GF LNVT +T++ Y S LQR+M++  +  PL   A     L+ S
Sbjct: 139 TTEEMNLLELDFLFGIGFQLNVTISTYNDYCSSLQREMVMRTMYSPLLEPAF----LVRS 198

Query: 189 SSRALKSHFCFDEDEASHKKQQLAA 211
             + L  +  +DED   H+  Q+ +
Sbjct: 199 FHKNLLKNL-YDED---HRNSQVTS 214

[Close]
BLAST of Chy6G108060 vs. ExPASy Swiss-Prot
Match: Q7XC35 (Cyclin-P4-1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYCP4-1 PE=2 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 2.2e-50
Identity = 109/210 (51.90%), Postives = 143/210 (68.10%), Query Frame = 0 

Query: 5   EDSTVMGKLIDFLSCLLQRVAESND---RNLSVNLQPHKISAFHGLTRPSISIQSYLDRI 64
           E +  + +++  LS LLQRVAE ND      +V  +   +SAF GLT+P+ISI  YL+RI
Sbjct: 5   EVAEAVPRVVAILSSLLQRVAERNDAAAAAAAVGEEAAAVSAFQGLTKPAISIGGYLERI 64

Query: 65  FKYANCSPCCFVIAYVYLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAYY 124
           F++ANCSP C+V+AY+YLDRF++R+P+L ++SFNVHRLLITSVL + KF+DD  YNNAY+
Sbjct: 65  FRFANCSPSCYVVAYIYLDRFLRRRPALAVDSFNVHRLLITSVLTAVKFVDDICYNNAYF 124

Query: 125 AKVGGISTTEMNFLEVDFLFGLGFHLNVTPTTFHSYYSYLQRQMLLL-QPPLTSTASTKS 184
           A+VGGIS  EMN+LEVDFLFG+ F LNVTP  F SY + LQ +M  L QPP         
Sbjct: 125 ARVGGISLMEMNYLEVDFLFGIAFDLNVTPAAFASYCAVLQSEMTYLEQPPAVDLP---- 184

Query: 185 DLLLSSSRALKSHFC-FDEDEAS--HKKQQ 208
                     + H C  D+D+A   HK+QQ
Sbjct: 185 ----------RLHCCPSDQDDAGCHHKQQQ 200

[Close]
BLAST of Chy6G108060 vs. ExPASy Swiss-Prot
Match: Q75HV0 (Cyclin-P3-1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYCP3-1 PE=3 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 1.2e-35
Identity = 86/205 (41.95%), Postives = 126/205 (61.46%), Query Frame = 0 

Query: 5   EDSTVMGKLIDFLSCLLQRVAESNDRNLSVNLQPHKISAFHGLTRPSISIQSYLDRIFKY 64
           +++T   K++  L+  L R  + N+  L  N      + FHG   P +SI+ Y +RIFKY
Sbjct: 30  KNNTEYPKVLLLLAAYLDRSVQKNEDLLDSNKIKDSSTIFHGHRAPDLSIKLYAERIFKY 89

Query: 65  ANCSPCCFVIAYVYLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAYYAKV 124
           + CSP CFV+A +Y++R++Q QP + + S +VHRLLITSV+V+AKF DD ++NNA+YA+V
Sbjct: 90  SECSPSCFVLALIYMERYLQ-QPHVYMTSLSVHRLLITSVVVAAKFTDDAFFNNAFYARV 149

Query: 125 GGISTTEMNFLEVDFLFGLGFHLNVTPTTFHSYYSYLQRQ--MLLLQPPLTST------- 184
           GGIST EMN LE+D LF L F L V   TF SY   L+++  +L++  P+          
Sbjct: 150 GGISTVEMNRLELDLLFNLDFRLKVDLETFGSYCLQLEKETMVLVIDRPIQQVHGVNSTK 209

Query: 185 ---------ASTKSDLLLSSSRALK 192
                     S KS+L+  SS+AL+
Sbjct: 210 DLSRNSSIDESCKSELMRYSSQALQ 233

[Close]
BLAST of Chy6G108060 vs. ExPASy TrEMBL
Match: A0A0A0L3W4 (Cyclin OS=Cucumis sativus OX=3659 GN=Csa_3G127030 PE=3 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 7.2e-113
Identity = 208/211 (98.58%), Postives = 210/211 (99.53%), Query Frame = 0 

Query: 1   MAELEDSTVMGKLIDFLSCLLQRVAESNDRNLSVNLQPHKISAFHGLTRPSISIQSYLDR 60
           MAELEDSTVM KLIDFLSCLLQRVAESNDRNLSVNLQPHKISAFHGLTRPSISIQSYLDR
Sbjct: 1   MAELEDSTVMAKLIDFLSCLLQRVAESNDRNLSVNLQPHKISAFHGLTRPSISIQSYLDR 60

Query: 61  IFKYANCSPCCFVIAYVYLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAY 120
           IFKYANCSPCCFVIAYVYLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAY
Sbjct: 61  IFKYANCSPCCFVIAYVYLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAY 120

Query: 121 YAKVGGISTTEMNFLEVDFLFGLGFHLNVTPTTFHSYYSYLQRQMLLLQPPLTSTASTKS 180
           YAKVGGISTTEMNFLEVDFLFGLGFHLNVTPTTFHSYYSYLQRQMLLLQPPLTS+ASTKS
Sbjct: 121 YAKVGGISTTEMNFLEVDFLFGLGFHLNVTPTTFHSYYSYLQRQMLLLQPPLTSSASTKS 180

Query: 181 DLLLSSSRALKSHFCFDEDEASHKKQQLAAV 212
           DLLL+SSRALKSHFCFDEDEASHKKQQLAAV
Sbjct: 181 DLLLTSSRALKSHFCFDEDEASHKKQQLAAV 211

[Close]
BLAST of Chy6G108060 vs. ExPASy TrEMBL
Match: A0A5A7U636 (Cyclin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003560 PE=3 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 5.2e-111
Identity = 205/211 (97.16%), Postives = 208/211 (98.58%), Query Frame = 0 

Query: 1   MAELEDSTVMGKLIDFLSCLLQRVAESNDRNLSVNLQPHKISAFHGLTRPSISIQSYLDR 60
           MAELED TVM KLIDFLSCLLQRVAESNDRNLSV+LQPHKIS+FHGLTRPSISIQSYLDR
Sbjct: 1   MAELEDPTVMAKLIDFLSCLLQRVAESNDRNLSVHLQPHKISSFHGLTRPSISIQSYLDR 60

Query: 61  IFKYANCSPCCFVIAYVYLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAY 120
           IFKYANCSPCCFVIAYVYLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAY
Sbjct: 61  IFKYANCSPCCFVIAYVYLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAY 120

Query: 121 YAKVGGISTTEMNFLEVDFLFGLGFHLNVTPTTFHSYYSYLQRQMLLLQPPLTSTASTKS 180
           YAKVGGISTTEMNFLEVDFLFGLGFHLNVTPTTFHSYYSYLQRQMLLLQPPL STASTKS
Sbjct: 121 YAKVGGISTTEMNFLEVDFLFGLGFHLNVTPTTFHSYYSYLQRQMLLLQPPLMSTASTKS 180

Query: 181 DLLLSSSRALKSHFCFDEDEASHKKQQLAAV 212
           +LLLSSSRALKSHFCFDEDEASHKKQQLAAV
Sbjct: 181 ELLLSSSRALKSHFCFDEDEASHKKQQLAAV 211

[Close]
BLAST of Chy6G108060 vs. ExPASy TrEMBL
Match: A0A1S3AWI3 (Cyclin OS=Cucumis melo OX=3656 GN=LOC103483393 PE=3 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 5.2e-111
Identity = 205/211 (97.16%), Postives = 208/211 (98.58%), Query Frame = 0 

Query: 1   MAELEDSTVMGKLIDFLSCLLQRVAESNDRNLSVNLQPHKISAFHGLTRPSISIQSYLDR 60
           MAELED TVM KLIDFLSCLLQRVAESNDRNLSV+LQPHKIS+FHGLTRPSISIQSYLDR
Sbjct: 1   MAELEDPTVMAKLIDFLSCLLQRVAESNDRNLSVHLQPHKISSFHGLTRPSISIQSYLDR 60

Query: 61  IFKYANCSPCCFVIAYVYLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAY 120
           IFKYANCSPCCFVIAYVYLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAY
Sbjct: 61  IFKYANCSPCCFVIAYVYLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAY 120

Query: 121 YAKVGGISTTEMNFLEVDFLFGLGFHLNVTPTTFHSYYSYLQRQMLLLQPPLTSTASTKS 180
           YAKVGGISTTEMNFLEVDFLFGLGFHLNVTPTTFHSYYSYLQRQMLLLQPPL STASTKS
Sbjct: 121 YAKVGGISTTEMNFLEVDFLFGLGFHLNVTPTTFHSYYSYLQRQMLLLQPPLMSTASTKS 180

Query: 181 DLLLSSSRALKSHFCFDEDEASHKKQQLAAV 212
           +LLLSSSRALKSHFCFDEDEASHKKQQLAAV
Sbjct: 181 ELLLSSSRALKSHFCFDEDEASHKKQQLAAV 211

[Close]
BLAST of Chy6G108060 vs. ExPASy TrEMBL
Match: A0A6J1IAK7 (Cyclin OS=Cucurbita maxima OX=3661 GN=LOC111472749 PE=3 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 2.8e-101
Identity = 190/211 (90.05%), Postives = 202/211 (95.73%), Query Frame = 0 

Query: 1   MAELEDSTVMGKLIDFLSCLLQRVAESNDRNLSVNLQPHKISAFHGLTRPSISIQSYLDR 60
           MAELED TVM KLI+FLSCLLQRVAESND+NLSV+LQ HKISAFHGLTRP+ISIQSYLDR
Sbjct: 1   MAELEDPTVMPKLINFLSCLLQRVAESNDQNLSVHLQHHKISAFHGLTRPAISIQSYLDR 60

Query: 61  IFKYANCSPCCFVIAYVYLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAY 120
           IFKYANCSPCCFV+AY+YLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAY
Sbjct: 61  IFKYANCSPCCFVVAYIYLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAY 120

Query: 121 YAKVGGISTTEMNFLEVDFLFGLGFHLNVTPTTFHSYYSYLQRQMLLLQPPLTSTASTKS 180
           YAKVGGIST E+NFLEVDFLFGLGFHLNVTP TFHSYYSYLQRQMLLLQPPL+ST S KS
Sbjct: 121 YAKVGGISTAEINFLEVDFLFGLGFHLNVTPNTFHSYYSYLQRQMLLLQPPLSST-SAKS 180

Query: 181 DLLLSSSRALKSHFCFDEDEASHKKQQLAAV 212
           +L+L ++RALK HFCFDEDEASHKKQQLAAV
Sbjct: 181 ELIL-TARALKPHFCFDEDEASHKKQQLAAV 209

[Close]
BLAST of Chy6G108060 vs. ExPASy TrEMBL
Match: A0A6J1IZ73 (Cyclin OS=Cucurbita maxima OX=3661 GN=LOC111479783 PE=3 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 2.4e-100
Identity = 190/211 (90.05%), Postives = 198/211 (93.84%), Query Frame = 0 

Query: 1   MAELEDSTVMGKLIDFLSCLLQRVAESNDRNLSVNLQPHKISAFHGLTRPSISIQSYLDR 60
           MAELED TVM KLIDFLSCLLQRVAESNDR+LSV+LQPHKISAFHGLTRP+ISIQSYLDR
Sbjct: 1   MAELEDPTVMAKLIDFLSCLLQRVAESNDRSLSVHLQPHKISAFHGLTRPTISIQSYLDR 60

Query: 61  IFKYANCSPCCFVIAYVYLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAY 120
           I KYANCSPCCFVIAYVYLDRFVQRQPS PINSFNVHRLLITSVL++AKFMDDT YNNAY
Sbjct: 61  ILKYANCSPCCFVIAYVYLDRFVQRQPSFPINSFNVHRLLITSVLIAAKFMDDTCYNNAY 120

Query: 121 YAKVGGISTTEMNFLEVDFLFGLGFHLNVTPTTFHSYYSYLQRQMLLLQPPLTSTASTKS 180
           YAKVGGIST EMNFLEVDFLFGLGF LNVTP TFHSYYSYLQRQMLLLQPPL S AS+KS
Sbjct: 121 YAKVGGISTIEMNFLEVDFLFGLGFRLNVTPATFHSYYSYLQRQMLLLQPPLMSAASSKS 180

Query: 181 DLLLSSSRALKSHFCFDEDEASHKKQQLAAV 212
           + LLSS+RALKSHFCFDEDEAS  KQQLAAV
Sbjct: 181 E-LLSSARALKSHFCFDEDEASLTKQQLAAV 210

[Close]
BLAST of Chy6G108060 vs. NCBI nr
Match: XP_004133942.1 (cyclin-U4-1 [Cucumis sativus] >KGN56640.1 hypothetical protein Csa_011200 [Cucumis sativus])

HSP 1 Score: 414 bits (1064), Expect = 1.01e-145
Identity = 208/211 (98.58%), Postives = 210/211 (99.53%), Query Frame = 0 

Query: 1   MAELEDSTVMGKLIDFLSCLLQRVAESNDRNLSVNLQPHKISAFHGLTRPSISIQSYLDR 60
           MAELEDSTVM KLIDFLSCLLQRVAESNDRNLSVNLQPHKISAFHGLTRPSISIQSYLDR
Sbjct: 1   MAELEDSTVMAKLIDFLSCLLQRVAESNDRNLSVNLQPHKISAFHGLTRPSISIQSYLDR 60

Query: 61  IFKYANCSPCCFVIAYVYLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAY 120
           IFKYANCSPCCFVIAYVYLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAY
Sbjct: 61  IFKYANCSPCCFVIAYVYLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAY 120

Query: 121 YAKVGGISTTEMNFLEVDFLFGLGFHLNVTPTTFHSYYSYLQRQMLLLQPPLTSTASTKS 180
           YAKVGGISTTEMNFLEVDFLFGLGFHLNVTPTTFHSYYSYLQRQMLLLQPPLTS+ASTKS
Sbjct: 121 YAKVGGISTTEMNFLEVDFLFGLGFHLNVTPTTFHSYYSYLQRQMLLLQPPLTSSASTKS 180

Query: 181 DLLLSSSRALKSHFCFDEDEASHKKQQLAAV 211
           DLLL+SSRALKSHFCFDEDEASHKKQQLAAV
Sbjct: 181 DLLLTSSRALKSHFCFDEDEASHKKQQLAAV 211

[Close]
BLAST of Chy6G108060 vs. NCBI nr
Match: XP_008438223.1 (PREDICTED: cyclin-U4-1 [Cucumis melo] >KAA0049071.1 cyclin-U4-1 [Cucumis melo var. makuwa] >TYK17491.1 cyclin-U4-1 [Cucumis melo var. makuwa])

HSP 1 Score: 408 bits (1048), Expect = 2.79e-143
Identity = 205/211 (97.16%), Postives = 208/211 (98.58%), Query Frame = 0 

Query: 1   MAELEDSTVMGKLIDFLSCLLQRVAESNDRNLSVNLQPHKISAFHGLTRPSISIQSYLDR 60
           MAELED TVM KLIDFLSCLLQRVAESNDRNLSV+LQPHKIS+FHGLTRPSISIQSYLDR
Sbjct: 1   MAELEDPTVMAKLIDFLSCLLQRVAESNDRNLSVHLQPHKISSFHGLTRPSISIQSYLDR 60

Query: 61  IFKYANCSPCCFVIAYVYLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAY 120
           IFKYANCSPCCFVIAYVYLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAY
Sbjct: 61  IFKYANCSPCCFVIAYVYLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAY 120

Query: 121 YAKVGGISTTEMNFLEVDFLFGLGFHLNVTPTTFHSYYSYLQRQMLLLQPPLTSTASTKS 180
           YAKVGGISTTEMNFLEVDFLFGLGFHLNVTPTTFHSYYSYLQRQMLLLQPPL STASTKS
Sbjct: 121 YAKVGGISTTEMNFLEVDFLFGLGFHLNVTPTTFHSYYSYLQRQMLLLQPPLMSTASTKS 180

Query: 181 DLLLSSSRALKSHFCFDEDEASHKKQQLAAV 211
           +LLLSSSRALKSHFCFDEDEASHKKQQLAAV
Sbjct: 181 ELLLSSSRALKSHFCFDEDEASHKKQQLAAV 211

[Close]
BLAST of Chy6G108060 vs. NCBI nr
Match: XP_038880049.1 (cyclin-U4-1 [Benincasa hispida])

HSP 1 Score: 390 bits (1003), Expect = 1.95e-136
Identity = 199/211 (94.31%), Postives = 204/211 (96.68%), Query Frame = 0 

Query: 1   MAELEDSTVMGKLIDFLSCLLQRVAESNDRNLSVNLQPHKISAFHGLTRPSISIQSYLDR 60
           MAELED TVM KLIDFLSCLLQRVAESND+NLSV+LQPHKISAFHGLTRP+ISIQSYL+R
Sbjct: 1   MAELEDPTVMAKLIDFLSCLLQRVAESNDQNLSVHLQPHKISAFHGLTRPTISIQSYLER 60

Query: 61  IFKYANCSPCCFVIAYVYLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAY 120
           IFKYANCSP CFVIAYVYLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAY
Sbjct: 61  IFKYANCSPSCFVIAYVYLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAY 120

Query: 121 YAKVGGISTTEMNFLEVDFLFGLGFHLNVTPTTFHSYYSYLQRQMLLLQPPLTSTASTKS 180
           YAKVGGISTTEMNFLEVDFLFGLGFHLNVTPTTFHSYYSYLQRQMLLLQPPL S ASTKS
Sbjct: 121 YAKVGGISTTEMNFLEVDFLFGLGFHLNVTPTTFHSYYSYLQRQMLLLQPPLMSAASTKS 180

Query: 181 DLLLSSSRALKSHFCFDEDEASHKKQQLAAV 211
           +L  SSSRALKSHFCFDEDEASHKKQQLAAV
Sbjct: 181 ELR-SSSRALKSHFCFDEDEASHKKQQLAAV 210

[Close]
BLAST of Chy6G108060 vs. NCBI nr
Match: XP_022974161.1 (cyclin-U4-1-like [Cucurbita maxima])

HSP 1 Score: 376 bits (965), Expect = 1.17e-130
Identity = 190/211 (90.05%), Postives = 202/211 (95.73%), Query Frame = 0 

Query: 1   MAELEDSTVMGKLIDFLSCLLQRVAESNDRNLSVNLQPHKISAFHGLTRPSISIQSYLDR 60
           MAELED TVM KLI+FLSCLLQRVAESND+NLSV+LQ HKISAFHGLTRP+ISIQSYLDR
Sbjct: 1   MAELEDPTVMPKLINFLSCLLQRVAESNDQNLSVHLQHHKISAFHGLTRPAISIQSYLDR 60

Query: 61  IFKYANCSPCCFVIAYVYLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAY 120
           IFKYANCSPCCFV+AY+YLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAY
Sbjct: 61  IFKYANCSPCCFVVAYIYLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAY 120

Query: 121 YAKVGGISTTEMNFLEVDFLFGLGFHLNVTPTTFHSYYSYLQRQMLLLQPPLTSTASTKS 180
           YAKVGGIST E+NFLEVDFLFGLGFHLNVTP TFHSYYSYLQRQMLLLQPPL+ST S KS
Sbjct: 121 YAKVGGISTAEINFLEVDFLFGLGFHLNVTPNTFHSYYSYLQRQMLLLQPPLSST-SAKS 180

Query: 181 DLLLSSSRALKSHFCFDEDEASHKKQQLAAV 211
           +L+L++ RALK HFCFDEDEASHKKQQLAAV
Sbjct: 181 ELILTA-RALKPHFCFDEDEASHKKQQLAAV 209

[Close]
BLAST of Chy6G108060 vs. NCBI nr
Match: KAG6597224.1 (Cyclin-U4-1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028696.1 Cyclin-U4-1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 375 bits (964), Expect = 1.66e-130
Identity = 190/211 (90.05%), Postives = 202/211 (95.73%), Query Frame = 0 

Query: 1   MAELEDSTVMGKLIDFLSCLLQRVAESNDRNLSVNLQPHKISAFHGLTRPSISIQSYLDR 60
           MAELED TVM KLI+FLSCLLQRVAESND+NLSV+LQ HKISAFHGLTRP+ISIQSYLDR
Sbjct: 1   MAELEDPTVMPKLINFLSCLLQRVAESNDQNLSVHLQHHKISAFHGLTRPAISIQSYLDR 60

Query: 61  IFKYANCSPCCFVIAYVYLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAY 120
           IFKYANCSPCCFV+AY+YLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAY
Sbjct: 61  IFKYANCSPCCFVVAYIYLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAY 120

Query: 121 YAKVGGISTTEMNFLEVDFLFGLGFHLNVTPTTFHSYYSYLQRQMLLLQPPLTSTASTKS 180
           YAKVGGIST E+NFLEVDFLFGLGFHLNVTP TFHSYYSYLQRQMLLLQPPL+S AS KS
Sbjct: 121 YAKVGGISTAEINFLEVDFLFGLGFHLNVTPNTFHSYYSYLQRQMLLLQPPLSS-ASAKS 180

Query: 181 DLLLSSSRALKSHFCFDEDEASHKKQQLAAV 211
           +L+L++ RALK HFCFDEDEASHKKQQLAAV
Sbjct: 181 ELILTA-RALKPHFCFDEDEASHKKQQLAAV 209

[Close]
BLAST of Chy6G108060 vs. TAIR 10
Match: AT2G44740.1 (cyclin p4;1 )

HSP 1 Score: 282.0 bits (720), Expect = 4.1e-76
Identity = 144/207 (69.57%), Postives = 170/207 (82.13%), Query Frame = 0 

Query: 1   MAELEDSTVMGKLIDFLSCLLQRVAESNDRNLSVNLQPHKISAFHGLTRPSISIQSYLDR 60
           MAELE+ +VM KLI FLS LL+RVAESND    V  Q  ++S FHGL+RP+I+IQSYL+R
Sbjct: 1   MAELENPSVMSKLIAFLSSLLERVAESNDLTRRVATQSQRVSVFHGLSRPTITIQSYLER 60

Query: 61  IFKYANCSPCCFVIAYVYLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAY 120
           IFKYANCSP CFV+AYVYLDRF  RQPSLPINSFNVHRLLITSV+V+AKF+DD YYNNAY
Sbjct: 61  IFKYANCSPSCFVVAYVYLDRFTHRQPSLPINSFNVHRLLITSVMVAAKFLDDLYYNNAY 120

Query: 121 YAKVGGISTTEMNFLEVDFLFGLGFHLNVTPTTFHSYYSYLQRQMLLLQPPLTSTASTKS 180
           YAKVGGIST EMNFLE+DFLFGLGF LNVTP TF++Y+SYLQ++M LLQP         S
Sbjct: 121 YAKVGGISTKEMNFLELDFLFGLGFELNVTPNTFNAYFSYLQKEMTLLQP--------LS 180

Query: 181 DLLLSSSRALKSHFCFDEDEASHKKQQ 208
            +++ SSR+L     F++DEASH+KQQ
Sbjct: 181 LVVVPSSRSL---ITFNDDEASHQKQQ 196

[Close]
BLAST of Chy6G108060 vs. TAIR 10
Match: AT5G61650.1 (CYCLIN P4;2 )

HSP 1 Score: 216.9 bits (551), Expect = 1.6e-56
Identity = 120/204 (58.82%), Postives = 152/204 (74.51%), Query Frame = 0 

Query: 9   VMGKLIDFLSCLLQRVAESNDRNLSVNLQPHKISAFHGLTRPSISIQSYLDRIFKYANCS 68
           +M  ++  +S LLQRV+E+ND NLS   Q  K S+F G+T+PSISI+SYL+RIF+YANCS
Sbjct: 21  IMPSVLTAMSYLLQRVSETND-NLS---QKQKPSSFTGVTKPSISIRSYLERIFEYANCS 80

Query: 69  PCCFVIAYVYLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAYYAKVGGIS 128
             C+++AY+YLDRFV++QP LPINSFNVHRL+ITSVLVSAKFMDD  YNN YYAKVGGIS
Sbjct: 81  YSCYIVAYIYLDRFVKKQPFLPINSFNVHRLIITSVLVSAKFMDDLSYNNEYYAKVGGIS 140

Query: 129 TTEMNFLEVDFLFGLGFHLNVTPTTFHSYYSYLQRQMLLLQPPLTSTASTKSDLLLSSSR 188
             EMN LE+DFLFG+GF LNVT +TF++Y  +LQR+M +L   + S     S   +SS  
Sbjct: 141 REEMNMLELDFLFGIGFELNVTVSTFNNYCCFLQREMAMLM-KMKSLFLEPSSFKISSKT 200

Query: 189 ALKSHFCFDEDEAS--HKKQQLAA 211
            L   +  +ED  S  H K+QLAA
Sbjct: 201 KLVM-YPHEEDSLSTHHNKKQLAA 218

[Close]
BLAST of Chy6G108060 vs. TAIR 10
Match: AT5G07450.1 (cyclin p4;3 )

HSP 1 Score: 207.6 bits (527), Expect = 9.8e-54
Identity = 114/205 (55.61%), Postives = 154/205 (75.12%), Query Frame = 0 

Query: 9   VMGKLIDFLSCLLQRVAESNDRNLSVNLQPHK-ISAFHGLTRPSISIQSYLDRIFKYANC 68
           +M  +I  +S LLQRV+E+ND +LS   + HK ISAF+ +T+PSISI+SY++RIFKYA+C
Sbjct: 19  IMPNVITAMSSLLQRVSETND-DLSRPFREHKRISAFNAVTKPSISIRSYMERIFKYADC 78

Query: 69  SPCCFVIAYVYLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAYYAKVGGI 128
           S  C+++AY+YLDRF+Q+QP LPI+S NVHRL+ITSVLVSAKFMDD  YNNA+YAKVGGI
Sbjct: 79  SDSCYIVAYIYLDRFIQKQPLLPIDSSNVHRLIITSVLVSAKFMDDLCYNNAFYAKVGGI 138

Query: 129 STTEMNFLEVDFLFGLGFHLNVTPTTFHSYYSYLQRQMLL--LQPPLTSTASTKSDLLLS 188
           +T EMN LE+DFLFG+GF LNVT +T++ Y S LQR+M++  +  PL   A     L+ S
Sbjct: 139 TTEEMNLLELDFLFGIGFQLNVTISTYNDYCSSLQREMVMRTMYSPLLEPAF----LVRS 198

Query: 189 SSRALKSHFCFDEDEASHKKQQLAA 211
             + L  +  +DED   H+  Q+ +
Sbjct: 199 FHKNLLKNL-YDED---HRNSQVTS 214

[Close]
BLAST of Chy6G108060 vs. TAIR 10
Match: AT3G63120.1 (cyclin p1;1 )

HSP 1 Score: 147.9 bits (372), Expect = 9.2e-36
Identity = 74/163 (45.40%), Postives = 109/163 (66.87%), Query Frame = 0 

Query: 9   VMGKLIDFLSCLLQRVAESNDRNLSVNL-------QPHKISAFHGLTRPSISIQSYLDRI 68
           + GK +     +L R++ S +R+L +N         P  ++ F G + P ISI  YLDRI
Sbjct: 23  IEGKRLKKPPTVLSRLSSSLERSLLLNHDDKILLGSPDSVTVFDGRSPPEISIAHYLDRI 82

Query: 69  FKYANCSPCCFVIAYVYLDRFVQRQPSLPINSFNVHRLLITSVLVSAKFMDDTYYNNAYY 128
           FKY+ CSP CFVIA++Y+D F+ +  +L +   NVHRL+IT+V+++AK  DD Y+NNAYY
Sbjct: 83  FKYSCCSPSCFVIAHIYIDHFLHKTRAL-LKPLNVHRLIITTVMLAAKVFDDRYFNNAYY 142

Query: 129 AKVGGISTTEMNFLEVDFLFGLGFHLNVTPTTFHSYYSYLQRQ 165
           A+VGG++T E+N LE++ LF L F L V P TFH++   L++Q
Sbjct: 143 ARVGGVTTRELNRLEMELLFTLDFKLQVDPQTFHTHCCQLEKQ 184

[Close]
BLAST of Chy6G108060 vs. TAIR 10
Match: AT3G21870.1 (cyclin p2;1 )

HSP 1 Score: 142.5 bits (358), Expect = 3.9e-34
Identity = 62/127 (48.82%), Postives = 90/127 (70.87%), Query Frame = 0 

Query: 41  ISAFHGLTRPSISIQSYLDRIFKYANCSPCCFVIAYVYLDRFVQRQPSLPINSFNVHRLL 100
           + AFHG+  PSISI  YL+RI+KY  CSP CFV+ YVY+DR   + P   + S NVHRLL
Sbjct: 56  LEAFHGVRAPSISIAKYLERIYKYTKCSPACFVVGYVYIDRLAHKHPGSLVVSLNVHRLL 115

Query: 101 ITSVLVSAKFMDDTYYNNAYYAKVGGISTTEMNFLEVDFLFGLGFHLNVTPTTFHSYYSY 160
           +T V+++AK +DD +YNN +YA+VGG+S  ++N +E++ LF L F + V+   F SY  +
Sbjct: 116 VTCVMIAAKILDDVHYNNEFYARVGGVSNADLNKMELELLFLLDFRVTVSFRVFESYCFH 175

Query: 161 LQRQMLL 168
           L+++M L
Sbjct: 176 LEKEMQL 182

The following BLAST results are available for this feature:
BLAST of Chy6G108060 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O805135.7e-7569.57Cyclin-U4-1 OS=Arabidopsis thaliana OX=3702 GN=CYCU4-1 PE=1 SV=1[more]
Q9FKF62.3e-5558.82Cyclin-U4-3 OS=Arabidopsis thaliana OX=3702 GN=CYCU4-3 PE=1 SV=1[more]
Q9LY161.4e-5255.61Cyclin-U4-2 OS=Arabidopsis thaliana OX=3702 GN=CYCU4-2 PE=1 SV=1[more]
Q7XC352.2e-5051.90Cyclin-P4-1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYCP4-1 PE=2 SV=1[more]
Q75HV01.2e-3541.95Cyclin-P3-1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYCP3-1 PE=3 SV=1[more]
BLAST of Chy6G108060 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L3W47.2e-11398.58Cyclin OS=Cucumis sativus OX=3659 GN=Csa_3G127030 PE=3 SV=1[more]
A0A5A7U6365.2e-11197.16Cyclin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003560 PE=3 S... [more]
A0A1S3AWI35.2e-11197.16Cyclin OS=Cucumis melo OX=3656 GN=LOC103483393 PE=3 SV=1[more]
A0A6J1IAK72.8e-10190.05Cyclin OS=Cucurbita maxima OX=3661 GN=LOC111472749 PE=3 SV=1[more]
A0A6J1IZ732.4e-10090.05Cyclin OS=Cucurbita maxima OX=3661 GN=LOC111479783 PE=3 SV=1[more]
BLAST of Chy6G108060 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004133942.11.01e-14598.58cyclin-U4-1 [Cucumis sativus] >KGN56640.1 hypothetical protein Csa_011200 [Cucum... [more]
XP_008438223.12.79e-14397.16PREDICTED: cyclin-U4-1 [Cucumis melo] >KAA0049071.1 cyclin-U4-1 [Cucumis melo va... [more]
XP_038880049.11.95e-13694.31cyclin-U4-1 [Benincasa hispida][more]
XP_022974161.11.17e-13090.05cyclin-U4-1-like [Cucurbita maxima][more]
KAG6597224.11.66e-13090.05Cyclin-U4-1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028696.1 Cycli... [more]
BLAST of Chy6G108060 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G44740.14.1e-7669.57cyclin p4;1 [more]
AT5G61650.11.6e-5658.82CYCLIN P4;2 [more]
AT5G07450.19.8e-5455.61cyclin p4;3 [more]
AT3G63120.19.2e-3645.40cyclin p1;1 [more]
AT3G21870.13.9e-3448.82cyclin p2;1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012389Cyclin P/UPIRSFPIRSF027110PREGcoord: 2..207
e-value: 1.7E-75
score: 251.4
IPR013922Cyclin PHO80-likePFAMPF08613Cyclincoord: 27..147
e-value: 4.0E-39
score: 134.6
NoneNo IPR availableGENE3D1.10.472.10coord: 5..207
e-value: 1.3E-56
score: 193.5
NoneNo IPR availablePANTHERPTHR15615:SF102CYCLINcoord: 1..208
NoneNo IPR availablePANTHERPTHR15615UNCHARACTERIZEDcoord: 1..208
IPR036915Cyclin-like superfamilySUPERFAMILY47954Cyclin-likecoord: 57..148

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy6G108060.1Chy6G108060.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000079 regulation of cyclin-dependent protein serine/threonine kinase activity
molecular_function GO:0016301 kinase activity
molecular_function GO:0019901 protein kinase binding