Chy6G107180 (gene) Cucumber (hystrix) v1

Overview
NameChy6G107180
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionMethenyltetrahydrofolate cyclohydrolase
LocationchrH06: 1252077 .. 1254334 (+)
RNA-Seq ExpressionChy6G107180
SyntenyChy6G107180
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGTCGTCGATGAAATTCACAGATTGCTCCCCTTCCGCCACTCTTCGTCTTTTTCCCTCTGCTCGAACCAGGCTTCGAAACTTCGATGGCGCTGTTCTCTTTCACCGTTTTCTTGACTGTTCGCGCCCTGCCTTCCCTAGAACTTTCGTTTCTCGCTCTTCGTCTTCGACTTTGCCCGTCATCTCTGGTATGCGTCTCTCTCTCCTTTTCTCCGTATTCGTTTTTGTTTATGAAAAAGTTTATTGATGATTTTTTTTAGAGCTTCGCGTTTTGAGGATCTGCCTTTTCATTTCTCATCTAATTTTGCTTCTTGAGCTTGCAGAAACTCTGGAATATTTTTGCGTTCATTTTAGGTGACTTCAAAGCTTTCTAGCTTTTAGAGTAATTGATTCTGAAATGGCGTTTAATTGTTTTAGTTTTCTATATTGGCTTGTCAACTTCCAAAGGACTTCTGTGTCTAGGCATGTATGTTGTGTAAGAATAATCTGGTTTGTTCATAGTTTTCTTTCTTAATGTATTTCTGTCAGTAAAAATTACCACGGAAAAGCCAAATCAGATTGATTTTTCTTTCTTCGAGTAGCGGAGATCTTTTTCGTAAGGGATTCTTAAGTCCTTGACTACTATTTTCTTTTTATTTCAGCTTCAATTTCAACCGAAGCAACTGCAGAAGTGATTGATGGAAAATTGGTGGCAAAGCAGATCAGAGAAGAGATTACTGCTGAAGTATCTCGAATGAAGGATGCCATTGGTGTTGTTCCAGGCTTAGCAGTCATCCTTGTAGGAGATAGGAAGGATTCTGCAACTTATGTACGAAATAAGAAAAAAGCTTGTGATTCTGTGGGTATTAAATCTTTTGAAGTAAACTTACCCGAAGATTGCACCGAGCAAGAAGTACTCAAGTATATATCTGACTTCAATGATGATCCATCCGTCCATGGAATTCTTGTTCAGTTACCTCTGCCTAAAGTACGTCACCAAAACTCCATTTCTTTTGCCCAATAGTTATCTTGTCACAATTTACTATACTATCTTAGTATATCACGAATGTTCCGGTAGTTTAGTTGAGATGCACATAAGCTGGTTTAACATTCACCGTCACAGATATATATCTATATATATATGAAGCACCCTAAATGTAAATTATCTGTTATCTTAAATAAGTTGTGGGTCTCGTCTTTCTTTAGAGCCTGCTTGTTTTATCGAATTAATTTTTAAAATAGTGTCTTCAATTTGTGCCCATGCCCTTTGGTCTATGGTTTATGTAAATGGAGATGAGAGTTTGAAATTGGATTTTACCTTGTTTCATTTGTAACTAGAACATAGCTGTAATGGTCTTGGGGCACAATGCTCACAAATACAATAGCAATTGAAGGTCAAGGATTACATGTTTATTAACTTTATGTTTTCATTGATTTGACAGCACATGAATGAACAGAATATATTGCATGCTGTTAGTATTGAAAAAGATGTAGATGGATTTCACCCACTAAACATCGGTCGTCTTGCAATGAGAGGTGAAGAACCATTGTTTGTTCCATGTACGCCAAAAGGGTGTATTGAATTGCTGCATAGGTATAACATTGGTATCAAAGGGAAGAGAGCCGTTGTAATAGGGCGAAGCAATATTGTAGGAACTCCAGCTGCTCTTTTGCTTCAAGTGAGATTTTATTTGTTAATCAATCATCTGTCATCATTCTATCAACTTCAATTACCGACTCCAATATCTACAATGCTATTGATTTTAGATTTTGTCTGCATTTATCATTTCGAGTAGGGAGAAGATGCAACGGTCAGCGTCGTTCATTCTAGAACCAAGGACCCTGAAGAGTTAACAAGACAAGCTGATATCATCATATCTGCAGTTGGCAGAGCCAATATGGTCAAGGGCAGTTGGATAAAGCCCGGAGCAGTTATTATTGACGTTGGAATCAATCCAGTAGAGGTGAGCGAAATAGTTTTTCTCCGAAGGGAATTGTGTACATTCAATATTTCACTTGCACTACTAATTTATATATCTTTGACCTTTGCTGTGAGTTTGTGTACATTCAATATTTCACTCTGATATTGTTATAATTATGTGATAGGATGCAAGTAGTCCTCGTGGATATCGGCTAGTTGGAGATGTTTGTTATGAGGAGGCCAGCAAAATTGCTTCAGCCATAACTCCGGTACCTGGGGGAGTTGGACCAATGACGATAGCGATGCTGCTTTCAAACACACTTACAGCAGCCAAAAGAACACATAACTTCCAGTAA

mRNA sequence

ATGGCGTCGTCGATGAAATTCACAGATTGCTCCCCTTCCGCCACTCTTCGTCTTTTTCCCTCTGCTCGAACCAGGCTTCGAAACTTCGATGGCGCTGTTCTCTTTCACCGTTTTCTTGACTCTTCAATTTCAACCGAAGCAACTGCAGAAGTGATTGATGGAAAATTGGTGGCAAAGCAGATCAGAGAAGAGATTACTGCTGAAGTATCTCGAATGAAGGATGCCATTGGTGTTGTTCCAGGCTTAGCAGTCATCCTTGTAGGAGATAGGAAGGATTCTGCAACTTATGTACGAAATAAGAAAAAAGCTTGTGATTCTGTGGGTATTAAATCTTTTGAAGTAAACTTACCCGAAGATTGCACCGAGCAAGAAGTACTCAAGTATATATCTGACTTCAATGATGATCCATCCGTCCATGGAATTCTTGTTCAGTTACCTCTGCCTAAACACATGAATGAACAGAATATATTGCATGCTGTTAGTATTGAAAAAGATGTAGATGGATTTCACCCACTAAACATCGGTCGTCTTGCAATGAGAGGTGAAGAACCATTGTTTGTTCCATGTACGCCAAAAGGGTGTATTGAATTGCTGCATAGGTATAACATTGGTATCAAAGGGAAGAGAGCCGTTGTAATAGGGCGAAGCAATATTGTAGGAACTCCAGCTGCTCTTTTGCTTCAAGGAGAAGATGCAACGGTCAGCGTCGTTCATTCTAGAACCAAGGACCCTGAAGAGTTAACAAGACAAGCTGATATCATCATATCTGCAGTTGGCAGAGCCAATATGGTCAAGGGCAGTTGGATAAAGCCCGGAGCAGTTATTATTGACGTTGGAATCAATCCAGTAGAGGATGCAAGTAGTCCTCGTGGATATCGGCTAGTTGGAGATGTTTGTTATGAGGAGGCCAGCAAAATTGCTTCAGCCATAACTCCGGTACCTGGGGGAGTTGGACCAATGACGATAGCGATGCTGCTTTCAAACACACTTACAGCAGCCAAAAGAACACATAACTTCCAGTAA

Coding sequence (CDS)

ATGGCGTCGTCGATGAAATTCACAGATTGCTCCCCTTCCGCCACTCTTCGTCTTTTTCCCTCTGCTCGAACCAGGCTTCGAAACTTCGATGGCGCTGTTCTCTTTCACCGTTTTCTTGACTCTTCAATTTCAACCGAAGCAACTGCAGAAGTGATTGATGGAAAATTGGTGGCAAAGCAGATCAGAGAAGAGATTACTGCTGAAGTATCTCGAATGAAGGATGCCATTGGTGTTGTTCCAGGCTTAGCAGTCATCCTTGTAGGAGATAGGAAGGATTCTGCAACTTATGTACGAAATAAGAAAAAAGCTTGTGATTCTGTGGGTATTAAATCTTTTGAAGTAAACTTACCCGAAGATTGCACCGAGCAAGAAGTACTCAAGTATATATCTGACTTCAATGATGATCCATCCGTCCATGGAATTCTTGTTCAGTTACCTCTGCCTAAACACATGAATGAACAGAATATATTGCATGCTGTTAGTATTGAAAAAGATGTAGATGGATTTCACCCACTAAACATCGGTCGTCTTGCAATGAGAGGTGAAGAACCATTGTTTGTTCCATGTACGCCAAAAGGGTGTATTGAATTGCTGCATAGGTATAACATTGGTATCAAAGGGAAGAGAGCCGTTGTAATAGGGCGAAGCAATATTGTAGGAACTCCAGCTGCTCTTTTGCTTCAAGGAGAAGATGCAACGGTCAGCGTCGTTCATTCTAGAACCAAGGACCCTGAAGAGTTAACAAGACAAGCTGATATCATCATATCTGCAGTTGGCAGAGCCAATATGGTCAAGGGCAGTTGGATAAAGCCCGGAGCAGTTATTATTGACGTTGGAATCAATCCAGTAGAGGATGCAAGTAGTCCTCGTGGATATCGGCTAGTTGGAGATGTTTGTTATGAGGAGGCCAGCAAAATTGCTTCAGCCATAACTCCGGTACCTGGGGGAGTTGGACCAATGACGATAGCGATGCTGCTTTCAAACACACTTACAGCAGCCAAAAGAACACATAACTTCCAGTAA

Protein sequence

MASSMKFTDCSPSATLRLFPSARTRLRNFDGAVLFHRFLDSSISTEATAEVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYVRNKKKACDSVGIKSFEVNLPEDCTEQEVLKYISDFNDDPSVHGILVQLPLPKHMNEQNILHAVSIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNIGIKGKRAVVIGRSNIVGTPAALLLQGEDATVSVVHSRTKDPEELTRQADIIISAVGRANMVKGSWIKPGAVIIDVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRTHNFQ*
Homology
BLAST of Chy6G107180 vs. ExPASy Swiss-Prot
Match: O65271 (Bifunctional protein FolD 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FOLD4 PE=1 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 1.5e-146
Identity = 271/359 (75.49%), Postives = 299/359 (83.29%), Query Frame = 0

Query: 3   SSMKFTDCSPSATLRLFPSARTR----LRNFDGAVLFHRFLD-------------SSIST 62
           +SM FTDCS + T RL    R+     LR   G +                    SSIS 
Sbjct: 2   ASMMFTDCSSTTTSRLIHLNRSSGTFLLRQCVGQLRLQTTASGRGCCIRSSSSPISSISA 61

Query: 63  EATAE----VIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYVRNKK 122
           +  +E    VIDGK VAK+IR+EIT EVSRMK++IGV+PGLAVILVGDRKDSATYVRNKK
Sbjct: 62  DTKSEGGAIVIDGKAVAKKIRDEITIEVSRMKESIGVIPGLAVILVGDRKDSATYVRNKK 121

Query: 123 KACDSVGIKSFEVNLPEDCTEQEVLKYISDFNDDPSVHGILVQLPLPKHMNEQNILHAVS 182
           KACDSVGIKSFEV L ED +E+EVLK +S FNDDPSVHGILVQLPLP HM+EQNIL+AVS
Sbjct: 122 KACDSVGIKSFEVRLAEDSSEEEVLKSVSGFNDDPSVHGILVQLPLPSHMDEQNILNAVS 181

Query: 183 IEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNIGIKGKRAVVIGRSNIVGT 242
           IEKDVDGFHPLNIGRLAMRG EPLFVPCTPKGCIELLHRYNI IKGKRAVVIGRSNIVG 
Sbjct: 182 IEKDVDGFHPLNIGRLAMRGREPLFVPCTPKGCIELLHRYNIEIKGKRAVVIGRSNIVGM 241

Query: 243 PAALLLQGEDATVSVVHSRTKDPEELTRQADIIISAVGRANMVKGSWIKPGAVIIDVGIN 302
           PAALLLQ EDATVS++HSRTK+PEE+TR+ADIIISAVG+ NMV+GSWIKPGAV+IDVGIN
Sbjct: 242 PAALLLQREDATVSIIHSRTKNPEEITREADIIISAVGQPNMVRGSWIKPGAVLIDVGIN 301

Query: 303 PVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRTHNFQ 341
           PVED S+ RGYRLVGD+CYEEASK+ASAITPVPGGVGPMTIAMLLSNTLT+AKR HNFQ
Sbjct: 302 PVEDPSAARGYRLVGDICYEEASKVASAITPVPGGVGPMTIAMLLSNTLTSAKRIHNFQ 360

BLAST of Chy6G107180 vs. ExPASy Swiss-Prot
Match: O65269 (Bifunctional protein FolD 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FOLD3 PE=3 SV=1)

HSP 1 Score: 416.0 bits (1068), Expect = 4.1e-115
Identity = 226/350 (64.57%), Postives = 251/350 (71.71%), Query Frame = 0

Query: 7   FTDCSPSATLRLFPSARTRLRNFDGAVL----------------FHRFLDSSISTEATAE 66
           FTDCS S T RL       L N +G  L                +   L    S+  +A 
Sbjct: 2   FTDCSSSTTSRLI-----HLYNRNGVFLPRPSVSQFSLRTTASTWRCTLSIRSSSSPSAI 61

Query: 67  VIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYVRNKKKACDSVGIK 126
           VIDGK  AK+IR++I  EVSRMK++IGVVP                              
Sbjct: 62  VIDGKAEAKKIRDDIKIEVSRMKESIGVVPA----------------------------- 121

Query: 127 SFEVNLPEDCTEQEVLKYISDFNDDPSVHGILVQLPLPKHMNEQNILHAVSIEKDVDGFH 186
                  ED +E+EVLKY+S FNDDPSVHG+LVQLPLP HM+EQNIL+AVSIEKDVDGFH
Sbjct: 122 -------EDSSEEEVLKYVSGFNDDPSVHGVLVQLPLPSHMDEQNILNAVSIEKDVDGFH 181

Query: 187 PLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNIGIKGKRAVVIGRSNIVGTPAALLLQGE 246
           PLNIGRLAMRG EPLFVPCTPKGCIELLHRYNI  KGKRAVVIGRSNIVG PAALLLQ E
Sbjct: 182 PLNIGRLAMRGREPLFVPCTPKGCIELLHRYNIEFKGKRAVVIGRSNIVGMPAALLLQKE 241

Query: 247 DATVSVVHSRTKDPEELTRQADIIISAVGRANMVKGSWIKPGAVIIDVGINPVEDASSPR 306
           DATVS++HSRT +PEELTRQADI+ISAVG+ NMV+GSWIKPGAV+IDVGI PVED S+  
Sbjct: 242 DATVSIIHSRTMNPEELTRQADILISAVGKPNMVRGSWIKPGAVLIDVGIKPVEDPSAAG 301

Query: 307 GYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRTHNFQ 341
           G RLVGD+CY EASKIASAITPVPG VGPMTIAMLLSNTLT+AKR HNFQ
Sbjct: 302 GERLVGDICYVEASKIASAITPVPGDVGPMTIAMLLSNTLTSAKRIHNFQ 310

BLAST of Chy6G107180 vs. ExPASy Swiss-Prot
Match: A2RVV7 (Bifunctional protein FolD 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FOLD1 PE=2 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 1.9e-112
Identity = 197/294 (67.01%), Postives = 235/294 (79.93%), Query Frame = 0

Query: 42  SISTEATAEVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYVRNKK 101
           S  TE    VIDG ++A++IR +I +EV +MK A+G VPGLAV+LVG+++DS TYVRNK 
Sbjct: 56  SFETEQKTVVIDGNVIAEEIRTKIISEVGKMKKAVGKVPGLAVVLVGEQRDSQTYVRNKI 115

Query: 102 KACDSVGIKSFEVNLPEDCTEQEVLKYISDFNDDPSVHGILVQLPLPKHMNEQNILHAVS 161
           KAC+  GIKS    LPEDCTE +++  +  FN+D S+HGILVQLPLP+H+NE  IL+ V 
Sbjct: 116 KACEETGIKSVLAELPEDCTEGQIISVLRKFNEDTSIHGILVQLPLPQHLNESKILNMVR 175

Query: 162 IEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNIGIKGKRAVVIGRSNIVGT 221
           +EKDVDGFHPLN+G LAMRG EPLFV CTPKGC+ELL R  + I GK AVVIGRSNIVG 
Sbjct: 176 LEKDVDGFHPLNVGNLAMRGREPLFVSCTPKGCVELLIRTGVEIAGKNAVVIGRSNIVGL 235

Query: 222 PAALLLQGEDATVSVVHSRTKDPEELTRQADIIISAVGRANMVKGSWIKPGAVIIDVGIN 281
           P +LLLQ  DATVS VH+ TKDPE +TR+ADI+I+A G  N+V+GSW+KPGAV+IDVG  
Sbjct: 236 PMSLLLQRHDATVSTVHAFTKDPEHITRKADIVIAAAGIPNLVRGSWLKPGAVVIDVGTT 295

Query: 282 PVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKR 336
           PVED+S   GYRLVGDVCYEEA  +ASAITPVPGGVGPMTI MLL NTL AAKR
Sbjct: 296 PVEDSSCEFGYRLVGDVCYEEALGVASAITPVPGGVGPMTITMLLCNTLEAAKR 349

BLAST of Chy6G107180 vs. ExPASy Swiss-Prot
Match: Q9LHH7 (Bifunctional protein FolD 2 OS=Arabidopsis thaliana OX=3702 GN=FOLD2 PE=2 SV=1)

HSP 1 Score: 391.7 bits (1005), Expect = 8.3e-108
Identity = 187/292 (64.04%), Postives = 233/292 (79.79%), Query Frame = 0

Query: 44  STEATAEVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYVRNKKKA 103
           S++ TA++IDGK +A  IR EI  EV  + +  G VPGLAV++VG RKDS TYV  K+KA
Sbjct: 4   SSDHTAKIIDGKAIAHTIRSEIAEEVRGLSEKHGKVPGLAVVIVGSRKDSQTYVNTKRKA 63

Query: 104 CDSVGIKSFEVNLPEDCTEQEVLKYISDFNDDPSVHGILVQLPLPKHMNEQNILHAVSIE 163
           C  VGIKSF+V LPE+ +E +++  + + N +P VHGILVQLPLPKH+NE++IL A+SI+
Sbjct: 64  CAEVGIKSFDVGLPEEVSEADLISKVHELNSNPDVHGILVQLPLPKHINEEHILGAISID 123

Query: 164 KDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNIGIKGKRAVVIGRSNIVGTPA 223
           KDVDGFHPLNIG+LAM+G EPLF+PCTPKGC+ELL R  + IKG+RAVV+GRSNIVG P 
Sbjct: 124 KDVDGFHPLNIGKLAMKGREPLFLPCTPKGCLELLARSGVKIKGQRAVVVGRSNIVGLPV 183

Query: 224 ALLLQGEDATVSVVHSRTKDPEELTRQADIIISAVGRANMVKGSWIKPGAVIIDVGINPV 283
           +LLL   DATV+ VHS TKDPE + R+ADI+I+A G+A+M+KG+WIKPGA +IDVG N V
Sbjct: 184 SLLLLKADATVTTVHSHTKDPEAIIREADIVIAACGQAHMIKGNWIKPGAAVIDVGTNAV 243

Query: 284 EDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKR 336
            D S   GYRLVGDV + EASK+A  ITPVPGGVGPMT+AMLL NT+  AKR
Sbjct: 244 SDPSKKSGYRLVGDVDFAEASKVAGFITPVPGGVGPMTVAMLLRNTVDGAKR 295

BLAST of Chy6G107180 vs. ExPASy Swiss-Prot
Match: A0LE04 (Bifunctional protein FolD OS=Magnetococcus marinus (strain ATCC BAA-1437 / JCM 17883 / MC-1) OX=156889 GN=folD PE=3 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 1.3e-84
Identity = 165/290 (56.90%), Postives = 200/290 (68.97%), Query Frame = 0

Query: 49  AEVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYVRNKKKACDSVG 108
           A VIDGK +A+ +REE+  EV R+K    + PGLAV+LVG    S  YVRNKK+AC++ G
Sbjct: 2   AHVIDGKAIAQSVREELRMEVERLKLNHQLTPGLAVVLVGADPASQVYVRNKKRACETAG 61

Query: 109 IKSFEVNLPEDCTEQEVLKYISDFNDDPSVHGILVQLPLPKHMNEQNILHAVSIEKDVDG 168
           I SF   L    ++ E+L  I   N D +VHGILVQLPLPKH++EQ +L A+S  KD DG
Sbjct: 62  IASFSHELAATTSQAELLALIEQLNQDDAVHGILVQLPLPKHIDEQKVLEAISPSKDADG 121

Query: 169 FHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNIGIKGKRAVVIGRSNIVGTPAALLLQ 228
           FHP N+GRL     +P F PCTP G +E+L    +  KGK AVVIGRSNIVG P AL+L 
Sbjct: 122 FHPYNVGRLVT--GDPTFQPCTPWGVMEMLKVSGVDPKGKHAVVIGRSNIVGKPVALMLL 181

Query: 229 GEDATVSVVHSRTKDPEELTRQADIIISAVGRANMVKGSWIKPGAVIIDVGINPVEDASS 288
              ATV++ HSRT D  E  ++ADI+++AVGRANMV GSWIK GAV+IDVGIN  ED   
Sbjct: 182 AAHATVTICHSRTPDLAETVKRADIVVAAVGRANMVPGSWIKKGAVVIDVGINRGEDG-- 241

Query: 289 PRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRTHN 339
               +L GDV Y    + ASAITPVPGGVGPMTIAMLL NT+  AKR HN
Sbjct: 242 ----KLCGDVDYASCFEHASAITPVPGGVGPMTIAMLLKNTVEGAKRAHN 283

BLAST of Chy6G107180 vs. ExPASy TrEMBL
Match: A0A0A0L8Y4 (Methenyltetrahydrofolate cyclohydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G122600 PE=3 SV=1)

HSP 1 Score: 646.4 bits (1666), Expect = 6.9e-182
Identity = 332/363 (91.46%), Postives = 337/363 (92.84%), Query Frame = 0

Query: 1   MASSMKFTDCSPSATLRLFPSARTRLRNFDGAVLFHRFLD-------------------- 60
           MASSMKFTDCSPS T+RLFPSARTRL +FDGAVLFHR LD                    
Sbjct: 1   MASSMKFTDCSPSTTVRLFPSARTRLPSFDGAVLFHRLLDRSRPAFPRTFVSRSSASTLP 60

Query: 61  ---SSISTEATAEVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYV 120
              +SISTEATAEVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYV
Sbjct: 61  VISASISTEATAEVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYV 120

Query: 121 RNKKKACDSVGIKSFEVNLPEDCTEQEVLKYISDFNDDPSVHGILVQLPLPKHMNEQNIL 180
           RNKKKAC+SVGIKSFEVNLPEDCTEQEVLKYISDFNDDPSVHGILVQLPLPKHMNEQNIL
Sbjct: 121 RNKKKACESVGIKSFEVNLPEDCTEQEVLKYISDFNDDPSVHGILVQLPLPKHMNEQNIL 180

Query: 181 HAVSIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNIGIKGKRAVVIGRSN 240
           HAVSIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNIGIKGKRAVVIGRSN
Sbjct: 181 HAVSIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNIGIKGKRAVVIGRSN 240

Query: 241 IVGTPAALLLQGEDATVSVVHSRTKDPEELTRQADIIISAVGRANMVKGSWIKPGAVIID 300
           IVGTPAALLLQGEDATVSVVHSRTKDPEELTRQADIIISAVG+ANMVKGSWIKPGAVIID
Sbjct: 241 IVGTPAALLLQGEDATVSVVHSRTKDPEELTRQADIIISAVGKANMVKGSWIKPGAVIID 300

Query: 301 VGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRTH 341
           VGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRTH
Sbjct: 301 VGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRTH 360

BLAST of Chy6G107180 vs. ExPASy TrEMBL
Match: A0A5A7TZL1 (Methenyltetrahydrofolate cyclohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G004520 PE=3 SV=1)

HSP 1 Score: 643.7 bits (1659), Expect = 4.5e-181
Identity = 330/363 (90.91%), Postives = 337/363 (92.84%), Query Frame = 0

Query: 1   MASSMKFTDCSPSATLRLFPSARTRLRNFDGAVLFHRFLD-------------------- 60
           MASSMKFTDCSPSAT+RLFPSARTR R+ DGAVLFHR LD                    
Sbjct: 1   MASSMKFTDCSPSATVRLFPSARTRFRSSDGAVLFHRLLDRSRPAFPRTFVSRSSASTLP 60

Query: 61  ---SSISTEATAEVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYV 120
              +SIST++TAEVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYV
Sbjct: 61  VISASISTQSTAEVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYV 120

Query: 121 RNKKKACDSVGIKSFEVNLPEDCTEQEVLKYISDFNDDPSVHGILVQLPLPKHMNEQNIL 180
           RNKKKAC+SVGIKSFEVNLPEDCTEQEVLKYISDFNDDPSVHGILVQLPLPKHMNEQNIL
Sbjct: 121 RNKKKACESVGIKSFEVNLPEDCTEQEVLKYISDFNDDPSVHGILVQLPLPKHMNEQNIL 180

Query: 181 HAVSIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNIGIKGKRAVVIGRSN 240
           HAVSIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNIGIKGKRAVVIGRSN
Sbjct: 181 HAVSIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNIGIKGKRAVVIGRSN 240

Query: 241 IVGTPAALLLQGEDATVSVVHSRTKDPEELTRQADIIISAVGRANMVKGSWIKPGAVIID 300
           IVGTPAALLLQGEDATVSVVHSRTKDPEELTRQADIIISAVG+ANMVKGSWIKPGAVIID
Sbjct: 241 IVGTPAALLLQGEDATVSVVHSRTKDPEELTRQADIIISAVGKANMVKGSWIKPGAVIID 300

Query: 301 VGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRTH 341
           VGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRTH
Sbjct: 301 VGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRTH 360

BLAST of Chy6G107180 vs. ExPASy TrEMBL
Match: A0A1S3AV55 (Methenyltetrahydrofolate cyclohydrolase OS=Cucumis melo OX=3656 GN=LOC103483291 PE=3 SV=1)

HSP 1 Score: 643.7 bits (1659), Expect = 4.5e-181
Identity = 330/363 (90.91%), Postives = 337/363 (92.84%), Query Frame = 0

Query: 1   MASSMKFTDCSPSATLRLFPSARTRLRNFDGAVLFHRFLD-------------------- 60
           MASSMKFTDCSPSAT+RLFPSARTR R+ DGAVLFHR LD                    
Sbjct: 1   MASSMKFTDCSPSATVRLFPSARTRFRSSDGAVLFHRLLDRSRPAFPRTFVSRSSASTLP 60

Query: 61  ---SSISTEATAEVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYV 120
              +SIST++TAEVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYV
Sbjct: 61  VISASISTQSTAEVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYV 120

Query: 121 RNKKKACDSVGIKSFEVNLPEDCTEQEVLKYISDFNDDPSVHGILVQLPLPKHMNEQNIL 180
           RNKKKAC+SVGIKSFEVNLPEDCTEQEVLKYISDFNDDPSVHGILVQLPLPKHMNEQNIL
Sbjct: 121 RNKKKACESVGIKSFEVNLPEDCTEQEVLKYISDFNDDPSVHGILVQLPLPKHMNEQNIL 180

Query: 181 HAVSIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNIGIKGKRAVVIGRSN 240
           HAVSIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNIGIKGKRAVVIGRSN
Sbjct: 181 HAVSIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNIGIKGKRAVVIGRSN 240

Query: 241 IVGTPAALLLQGEDATVSVVHSRTKDPEELTRQADIIISAVGRANMVKGSWIKPGAVIID 300
           IVGTPAALLLQGEDATVSVVHSRTKDPEELTRQADIIISAVG+ANMVKGSWIKPGAVIID
Sbjct: 241 IVGTPAALLLQGEDATVSVVHSRTKDPEELTRQADIIISAVGKANMVKGSWIKPGAVIID 300

Query: 301 VGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRTH 341
           VGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRTH
Sbjct: 301 VGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRTH 360

BLAST of Chy6G107180 vs. ExPASy TrEMBL
Match: A0A6J1GAU1 (Methenyltetrahydrofolate cyclohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111452457 PE=3 SV=1)

HSP 1 Score: 620.2 bits (1598), Expect = 5.3e-174
Identity = 320/363 (88.15%), Postives = 330/363 (90.91%), Query Frame = 0

Query: 1   MASSMKFTDCSPSATLRLFPSARTRLRNFDGAVLFHRFL--------------------- 60
           MASSMKFTDCSPSA +RL PS RTRLR+ D AVLFHR L                     
Sbjct: 1   MASSMKFTDCSPSAIVRLVPSTRTRLRSSDSAVLFHRVLGRSCPAYPRTFVSRSSISTLP 60

Query: 61  --DSSISTEATAEVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYV 120
              +SISTEATA+VIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYV
Sbjct: 61  VISASISTEATAQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYV 120

Query: 121 RNKKKACDSVGIKSFEVNLPEDCTEQEVLKYISDFNDDPSVHGILVQLPLPKHMNEQNIL 180
           RNKKKAC+SVGIKSFEVNLPE+ TEQEVLK+ISDFNDDPSVHGILVQLPLPKHMNEQN+L
Sbjct: 121 RNKKKACESVGIKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQNVL 180

Query: 181 HAVSIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNIGIKGKRAVVIGRSN 240
           HAVSIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNI IKGKRAVVIGRSN
Sbjct: 181 HAVSIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRSN 240

Query: 241 IVGTPAALLLQGEDATVSVVHSRTKDPEELTRQADIIISAVGRANMVKGSWIKPGAVIID 300
           IVGTPAALLLQ EDATVSVVHSRTKDPEELTRQADIIISAVG+ANMV+GSWIKPGAVIID
Sbjct: 241 IVGTPAALLLQREDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVIID 300

Query: 301 VGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRTH 341
           VGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRTH
Sbjct: 301 VGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRTH 360

BLAST of Chy6G107180 vs. ExPASy TrEMBL
Match: A0A6J1IDA1 (Methenyltetrahydrofolate cyclohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111474082 PE=3 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 5.8e-173
Identity = 320/364 (87.91%), Postives = 330/364 (90.66%), Query Frame = 0

Query: 1   MASSMKFTDCSPSATLRLFPSARTRLRNF-DGAVLFHRFL-------------------- 60
           MASSMKFTDCSPSA +RL PS RTRLR+F DGAVLFHR L                    
Sbjct: 1   MASSMKFTDCSPSAIVRLVPSTRTRLRSFSDGAVLFHRVLGRSCPAYPRTFVSRSSISTL 60

Query: 61  ---DSSISTEATAEVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATY 120
               +SISTEATA+VIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATY
Sbjct: 61  PVISASISTEATAQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATY 120

Query: 121 VRNKKKACDSVGIKSFEVNLPEDCTEQEVLKYISDFNDDPSVHGILVQLPLPKHMNEQNI 180
           VRNKKKAC+SVGIKSFEVNLPE+ TEQEVLK+ISDFNDDPSVHGILVQLPLPKHMNEQN+
Sbjct: 121 VRNKKKACESVGIKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQNV 180

Query: 181 LHAVSIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNIGIKGKRAVVIGRS 240
           LHAV IEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNI IKGKRAVVIGRS
Sbjct: 181 LHAVRIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRS 240

Query: 241 NIVGTPAALLLQGEDATVSVVHSRTKDPEELTRQADIIISAVGRANMVKGSWIKPGAVII 300
           NIVGTPAALLLQ EDATVSVVHSRTKDPEELTRQADIIISAVG+ANMV+GSWIKPGAVII
Sbjct: 241 NIVGTPAALLLQREDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVII 300

Query: 301 DVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRT 341
           DVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKR 
Sbjct: 301 DVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRK 360

BLAST of Chy6G107180 vs. NCBI nr
Match: XP_004134331.1 (bifunctional protein FolD 4, chloroplastic [Cucumis sativus] >KGN56536.1 hypothetical protein Csa_011126 [Cucumis sativus])

HSP 1 Score: 647 bits (1668), Expect = 4.28e-233
Identity = 332/363 (91.46%), Postives = 337/363 (92.84%), Query Frame = 0

Query: 1   MASSMKFTDCSPSATLRLFPSARTRLRNFDGAVLFHRFLD-------------------- 60
           MASSMKFTDCSPS T+RLFPSARTRL +FDGAVLFHR LD                    
Sbjct: 1   MASSMKFTDCSPSTTVRLFPSARTRLPSFDGAVLFHRLLDRSRPAFPRTFVSRSSASTLP 60

Query: 61  ---SSISTEATAEVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYV 120
              +SISTEATAEVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYV
Sbjct: 61  VISASISTEATAEVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYV 120

Query: 121 RNKKKACDSVGIKSFEVNLPEDCTEQEVLKYISDFNDDPSVHGILVQLPLPKHMNEQNIL 180
           RNKKKAC+SVGIKSFEVNLPEDCTEQEVLKYISDFNDDPSVHGILVQLPLPKHMNEQNIL
Sbjct: 121 RNKKKACESVGIKSFEVNLPEDCTEQEVLKYISDFNDDPSVHGILVQLPLPKHMNEQNIL 180

Query: 181 HAVSIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNIGIKGKRAVVIGRSN 240
           HAVSIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNIGIKGKRAVVIGRSN
Sbjct: 181 HAVSIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNIGIKGKRAVVIGRSN 240

Query: 241 IVGTPAALLLQGEDATVSVVHSRTKDPEELTRQADIIISAVGRANMVKGSWIKPGAVIID 300
           IVGTPAALLLQGEDATVSVVHSRTKDPEELTRQADIIISAVG+ANMVKGSWIKPGAVIID
Sbjct: 241 IVGTPAALLLQGEDATVSVVHSRTKDPEELTRQADIIISAVGKANMVKGSWIKPGAVIID 300

Query: 301 VGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRTH 340
           VGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRTH
Sbjct: 301 VGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRTH 360

BLAST of Chy6G107180 vs. NCBI nr
Match: XP_008438072.1 (PREDICTED: bifunctional protein FolD 4, chloroplastic isoform X1 [Cucumis melo] >KAA0048982.1 bifunctional protein FolD 4 [Cucumis melo var. makuwa] >TYK17582.1 bifunctional protein FolD 4 [Cucumis melo var. makuwa])

HSP 1 Score: 644 bits (1661), Expect = 4.99e-232
Identity = 330/363 (90.91%), Postives = 337/363 (92.84%), Query Frame = 0

Query: 1   MASSMKFTDCSPSATLRLFPSARTRLRNFDGAVLFHRFLD-------------------- 60
           MASSMKFTDCSPSAT+RLFPSARTR R+ DGAVLFHR LD                    
Sbjct: 1   MASSMKFTDCSPSATVRLFPSARTRFRSSDGAVLFHRLLDRSRPAFPRTFVSRSSASTLP 60

Query: 61  ---SSISTEATAEVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYV 120
              +SIST++TAEVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYV
Sbjct: 61  VISASISTQSTAEVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYV 120

Query: 121 RNKKKACDSVGIKSFEVNLPEDCTEQEVLKYISDFNDDPSVHGILVQLPLPKHMNEQNIL 180
           RNKKKAC+SVGIKSFEVNLPEDCTEQEVLKYISDFNDDPSVHGILVQLPLPKHMNEQNIL
Sbjct: 121 RNKKKACESVGIKSFEVNLPEDCTEQEVLKYISDFNDDPSVHGILVQLPLPKHMNEQNIL 180

Query: 181 HAVSIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNIGIKGKRAVVIGRSN 240
           HAVSIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNIGIKGKRAVVIGRSN
Sbjct: 181 HAVSIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNIGIKGKRAVVIGRSN 240

Query: 241 IVGTPAALLLQGEDATVSVVHSRTKDPEELTRQADIIISAVGRANMVKGSWIKPGAVIID 300
           IVGTPAALLLQGEDATVSVVHSRTKDPEELTRQADIIISAVG+ANMVKGSWIKPGAVIID
Sbjct: 241 IVGTPAALLLQGEDATVSVVHSRTKDPEELTRQADIIISAVGKANMVKGSWIKPGAVIID 300

Query: 301 VGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRTH 340
           VGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRTH
Sbjct: 301 VGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRTH 360

BLAST of Chy6G107180 vs. NCBI nr
Match: XP_023528480.1 (bifunctional protein FolD 4, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 629 bits (1621), Expect = 6.21e-226
Identity = 324/363 (89.26%), Postives = 330/363 (90.91%), Query Frame = 0

Query: 1   MASSMKFTDCSPSATLRLFPSARTRLRNFDGAVLFHRFL--------------------- 60
           MASSMKFTDCSP AT+RL PS RTRLRN DGA LFHR L                     
Sbjct: 1   MASSMKFTDCSPCATVRLVPSTRTRLRNSDGAGLFHRLLGLSRPAFPRTFVSRSSTSTLP 60

Query: 61  --DSSISTEATAEVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYV 120
              +SISTEATAEVIDGKLVAKQIREEITAEVSRMK AIGVVPGLAVILVGDRKDSATYV
Sbjct: 61  VISASISTEATAEVIDGKLVAKQIREEITAEVSRMKGAIGVVPGLAVILVGDRKDSATYV 120

Query: 121 RNKKKACDSVGIKSFEVNLPEDCTEQEVLKYISDFNDDPSVHGILVQLPLPKHMNEQNIL 180
           RNKKKAC+SVGIKSFEVNLPEDC+EQEVLKYISDFNDDPSVHGILVQLPLPKHMNEQNIL
Sbjct: 121 RNKKKACESVGIKSFEVNLPEDCSEQEVLKYISDFNDDPSVHGILVQLPLPKHMNEQNIL 180

Query: 181 HAVSIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNIGIKGKRAVVIGRSN 240
           HAVSIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNI IKGKRAVVIGRSN
Sbjct: 181 HAVSIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRSN 240

Query: 241 IVGTPAALLLQGEDATVSVVHSRTKDPEELTRQADIIISAVGRANMVKGSWIKPGAVIID 300
           IVGTPAALLLQGEDATVSVVHSRTKDPEELT+QADIIISAVG+ANMVKGSWIKPGAVIID
Sbjct: 241 IVGTPAALLLQGEDATVSVVHSRTKDPEELTKQADIIISAVGKANMVKGSWIKPGAVIID 300

Query: 301 VGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRTH 340
           VGINPVEDASSPRGYRLVGDVCYEEA KIASAITPVPGGVGPMTIAMLLSNTLTAAKRTH
Sbjct: 301 VGINPVEDASSPRGYRLVGDVCYEEAIKIASAITPVPGGVGPMTIAMLLSNTLTAAKRTH 360

BLAST of Chy6G107180 vs. NCBI nr
Match: KAG6582599.1 (Bifunctional protein FolD 4, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 627 bits (1616), Expect = 3.59e-225
Identity = 323/363 (88.98%), Postives = 330/363 (90.91%), Query Frame = 0

Query: 1   MASSMKFTDCSPSATLRLFPSARTRLRNFDGAVLFHRFL--------------------- 60
           MASSMKFTDCSP AT+RL PS RTRLRN DGA LFHR L                     
Sbjct: 1   MASSMKFTDCSPCATVRLVPSTRTRLRNSDGAGLFHRLLGLSRPAFPRTFVSRSSTSTLP 60

Query: 61  --DSSISTEATAEVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYV 120
              +SISTEATAEVIDGKLVAKQIREEITAEVSRMK AIGVVPGLAVILVGDRKDSATYV
Sbjct: 61  VISASISTEATAEVIDGKLVAKQIREEITAEVSRMKGAIGVVPGLAVILVGDRKDSATYV 120

Query: 121 RNKKKACDSVGIKSFEVNLPEDCTEQEVLKYISDFNDDPSVHGILVQLPLPKHMNEQNIL 180
           RNKKKAC+SVGIKSFEVNLPEDCTEQEVLKYIS+FNDDPSVHGILVQLPLPKHMNEQNIL
Sbjct: 121 RNKKKACESVGIKSFEVNLPEDCTEQEVLKYISNFNDDPSVHGILVQLPLPKHMNEQNIL 180

Query: 181 HAVSIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNIGIKGKRAVVIGRSN 240
           HAVSIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNI IKGKRAVVIGRSN
Sbjct: 181 HAVSIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRSN 240

Query: 241 IVGTPAALLLQGEDATVSVVHSRTKDPEELTRQADIIISAVGRANMVKGSWIKPGAVIID 300
           IVGTPAALLLQGEDATVSVVHSRTKDPEELT+QADIIISAVG+ANMVKGSWIKPGAVIID
Sbjct: 241 IVGTPAALLLQGEDATVSVVHSRTKDPEELTKQADIIISAVGKANMVKGSWIKPGAVIID 300

Query: 301 VGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRTH 340
           VGINPVEDASSPRG+RLVGDVCYEEA KIASAITPVPGGVGPMTIAMLLSNTLTAAKRTH
Sbjct: 301 VGINPVEDASSPRGFRLVGDVCYEEAIKIASAITPVPGGVGPMTIAMLLSNTLTAAKRTH 360

BLAST of Chy6G107180 vs. NCBI nr
Match: XP_038879177.1 (bifunctional protein FolD 4, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 625 bits (1613), Expect = 1.03e-224
Identity = 322/363 (88.71%), Postives = 330/363 (90.91%), Query Frame = 0

Query: 1   MASSMKFTDCSPSATLRLFPSARTRLRNFDGAVLFHRFL--------------------- 60
           MASSMKFTDCSPSA +RLFPS RTRLR+ D AVLF R L                     
Sbjct: 1   MASSMKFTDCSPSAAVRLFPSTRTRLRSSDDAVLFRRLLGLSHPAFPRTFVSRSSTSTLP 60

Query: 61  --DSSISTEATAEVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYV 120
              +SISTEATAEVIDGKLVAKQIREEITAEVSRMK+AIGVVPGLAVILVGDRKDSATYV
Sbjct: 61  VISASISTEATAEVIDGKLVAKQIREEITAEVSRMKEAIGVVPGLAVILVGDRKDSATYV 120

Query: 121 RNKKKACDSVGIKSFEVNLPEDCTEQEVLKYISDFNDDPSVHGILVQLPLPKHMNEQNIL 180
           RNKKKAC+SVGIKSFEVNLPEDCTEQEVLKYISDFNDDP VHGILVQLPLPKHMNEQNIL
Sbjct: 121 RNKKKACESVGIKSFEVNLPEDCTEQEVLKYISDFNDDPHVHGILVQLPLPKHMNEQNIL 180

Query: 181 HAVSIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNIGIKGKRAVVIGRSN 240
           HAV+I KDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNI IKGKRAVVIGRSN
Sbjct: 181 HAVNIGKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRSN 240

Query: 241 IVGTPAALLLQGEDATVSVVHSRTKDPEELTRQADIIISAVGRANMVKGSWIKPGAVIID 300
           IVGTPAALLLQGEDATVSVVHSRTKDPEELTRQADIIISAVG+ANMVKGSWIKPGAVIID
Sbjct: 241 IVGTPAALLLQGEDATVSVVHSRTKDPEELTRQADIIISAVGKANMVKGSWIKPGAVIID 300

Query: 301 VGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRTH 340
           VGINPVEDA+SPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRTH
Sbjct: 301 VGINPVEDANSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRTH 360

BLAST of Chy6G107180 vs. TAIR 10
Match: AT4G00620.1 (Amino acid dehydrogenase family protein )

HSP 1 Score: 520.4 bits (1339), Expect = 1.1e-147
Identity = 271/359 (75.49%), Postives = 299/359 (83.29%), Query Frame = 0

Query: 3   SSMKFTDCSPSATLRLFPSARTR----LRNFDGAVLFHRFLD-------------SSIST 62
           +SM FTDCS + T RL    R+     LR   G +                    SSIS 
Sbjct: 2   ASMMFTDCSSTTTSRLIHLNRSSGTFLLRQCVGQLRLQTTASGRGCCIRSSSSPISSISA 61

Query: 63  EATAE----VIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYVRNKK 122
           +  +E    VIDGK VAK+IR+EIT EVSRMK++IGV+PGLAVILVGDRKDSATYVRNKK
Sbjct: 62  DTKSEGGAIVIDGKAVAKKIRDEITIEVSRMKESIGVIPGLAVILVGDRKDSATYVRNKK 121

Query: 123 KACDSVGIKSFEVNLPEDCTEQEVLKYISDFNDDPSVHGILVQLPLPKHMNEQNILHAVS 182
           KACDSVGIKSFEV L ED +E+EVLK +S FNDDPSVHGILVQLPLP HM+EQNIL+AVS
Sbjct: 122 KACDSVGIKSFEVRLAEDSSEEEVLKSVSGFNDDPSVHGILVQLPLPSHMDEQNILNAVS 181

Query: 183 IEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNIGIKGKRAVVIGRSNIVGT 242
           IEKDVDGFHPLNIGRLAMRG EPLFVPCTPKGCIELLHRYNI IKGKRAVVIGRSNIVG 
Sbjct: 182 IEKDVDGFHPLNIGRLAMRGREPLFVPCTPKGCIELLHRYNIEIKGKRAVVIGRSNIVGM 241

Query: 243 PAALLLQGEDATVSVVHSRTKDPEELTRQADIIISAVGRANMVKGSWIKPGAVIIDVGIN 302
           PAALLLQ EDATVS++HSRTK+PEE+TR+ADIIISAVG+ NMV+GSWIKPGAV+IDVGIN
Sbjct: 242 PAALLLQREDATVSIIHSRTKNPEEITREADIIISAVGQPNMVRGSWIKPGAVLIDVGIN 301

Query: 303 PVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRTHNFQ 341
           PVED S+ RGYRLVGD+CYEEASK+ASAITPVPGGVGPMTIAMLLSNTLT+AKR HNFQ
Sbjct: 302 PVEDPSAARGYRLVGDICYEEASKVASAITPVPGGVGPMTIAMLLSNTLTSAKRIHNFQ 360

BLAST of Chy6G107180 vs. TAIR 10
Match: AT4G00600.1 (Amino acid dehydrogenase family protein )

HSP 1 Score: 416.0 bits (1068), Expect = 2.9e-116
Identity = 226/350 (64.57%), Postives = 251/350 (71.71%), Query Frame = 0

Query: 7   FTDCSPSATLRLFPSARTRLRNFDGAVL----------------FHRFLDSSISTEATAE 66
           FTDCS S T RL       L N +G  L                +   L    S+  +A 
Sbjct: 2   FTDCSSSTTSRLI-----HLYNRNGVFLPRPSVSQFSLRTTASTWRCTLSIRSSSSPSAI 61

Query: 67  VIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYVRNKKKACDSVGIK 126
           VIDGK  AK+IR++I  EVSRMK++IGVVP                              
Sbjct: 62  VIDGKAEAKKIRDDIKIEVSRMKESIGVVPA----------------------------- 121

Query: 127 SFEVNLPEDCTEQEVLKYISDFNDDPSVHGILVQLPLPKHMNEQNILHAVSIEKDVDGFH 186
                  ED +E+EVLKY+S FNDDPSVHG+LVQLPLP HM+EQNIL+AVSIEKDVDGFH
Sbjct: 122 -------EDSSEEEVLKYVSGFNDDPSVHGVLVQLPLPSHMDEQNILNAVSIEKDVDGFH 181

Query: 187 PLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNIGIKGKRAVVIGRSNIVGTPAALLLQGE 246
           PLNIGRLAMRG EPLFVPCTPKGCIELLHRYNI  KGKRAVVIGRSNIVG PAALLLQ E
Sbjct: 182 PLNIGRLAMRGREPLFVPCTPKGCIELLHRYNIEFKGKRAVVIGRSNIVGMPAALLLQKE 241

Query: 247 DATVSVVHSRTKDPEELTRQADIIISAVGRANMVKGSWIKPGAVIIDVGINPVEDASSPR 306
           DATVS++HSRT +PEELTRQADI+ISAVG+ NMV+GSWIKPGAV+IDVGI PVED S+  
Sbjct: 242 DATVSIIHSRTMNPEELTRQADILISAVGKPNMVRGSWIKPGAVLIDVGIKPVEDPSAAG 301

Query: 307 GYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRTHNFQ 341
           G RLVGD+CY EASKIASAITPVPG VGPMTIAMLLSNTLT+AKR HNFQ
Sbjct: 302 GERLVGDICYVEASKIASAITPVPGDVGPMTIAMLLSNTLTSAKRIHNFQ 310

BLAST of Chy6G107180 vs. TAIR 10
Match: AT2G38660.1 (Amino acid dehydrogenase family protein )

HSP 1 Score: 407.1 bits (1045), Expect = 1.4e-113
Identity = 197/294 (67.01%), Postives = 235/294 (79.93%), Query Frame = 0

Query: 42  SISTEATAEVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYVRNKK 101
           S  TE    VIDG ++A++IR +I +EV +MK A+G VPGLAV+LVG+++DS TYVRNK 
Sbjct: 56  SFETEQKTVVIDGNVIAEEIRTKIISEVGKMKKAVGKVPGLAVVLVGEQRDSQTYVRNKI 115

Query: 102 KACDSVGIKSFEVNLPEDCTEQEVLKYISDFNDDPSVHGILVQLPLPKHMNEQNILHAVS 161
           KAC+  GIKS    LPEDCTE +++  +  FN+D S+HGILVQLPLP+H+NE  IL+ V 
Sbjct: 116 KACEETGIKSVLAELPEDCTEGQIISVLRKFNEDTSIHGILVQLPLPQHLNESKILNMVR 175

Query: 162 IEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNIGIKGKRAVVIGRSNIVGT 221
           +EKDVDGFHPLN+G LAMRG EPLFV CTPKGC+ELL R  + I GK AVVIGRSNIVG 
Sbjct: 176 LEKDVDGFHPLNVGNLAMRGREPLFVSCTPKGCVELLIRTGVEIAGKNAVVIGRSNIVGL 235

Query: 222 PAALLLQGEDATVSVVHSRTKDPEELTRQADIIISAVGRANMVKGSWIKPGAVIIDVGIN 281
           P +LLLQ  DATVS VH+ TKDPE +TR+ADI+I+A G  N+V+GSW+KPGAV+IDVG  
Sbjct: 236 PMSLLLQRHDATVSTVHAFTKDPEHITRKADIVIAAAGIPNLVRGSWLKPGAVVIDVGTT 295

Query: 282 PVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKR 336
           PVED+S   GYRLVGDVCYEEA  +ASAITPVPGGVGPMTI MLL NTL AAKR
Sbjct: 296 PVEDSSCEFGYRLVGDVCYEEALGVASAITPVPGGVGPMTITMLLCNTLEAAKR 349

BLAST of Chy6G107180 vs. TAIR 10
Match: AT2G38660.3 (Amino acid dehydrogenase family protein )

HSP 1 Score: 407.1 bits (1045), Expect = 1.4e-113
Identity = 197/294 (67.01%), Postives = 235/294 (79.93%), Query Frame = 0

Query: 42  SISTEATAEVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYVRNKK 101
           S  TE    VIDG ++A++IR +I +EV +MK A+G VPGLAV+LVG+++DS TYVRNK 
Sbjct: 56  SFETEQKTVVIDGNVIAEEIRTKIISEVGKMKKAVGKVPGLAVVLVGEQRDSQTYVRNKI 115

Query: 102 KACDSVGIKSFEVNLPEDCTEQEVLKYISDFNDDPSVHGILVQLPLPKHMNEQNILHAVS 161
           KAC+  GIKS    LPEDCTE +++  +  FN+D S+HGILVQLPLP+H+NE  IL+ V 
Sbjct: 116 KACEETGIKSVLAELPEDCTEGQIISVLRKFNEDTSIHGILVQLPLPQHLNESKILNMVR 175

Query: 162 IEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNIGIKGKRAVVIGRSNIVGT 221
           +EKDVDGFHPLN+G LAMRG EPLFV CTPKGC+ELL R  + I GK AVVIGRSNIVG 
Sbjct: 176 LEKDVDGFHPLNVGNLAMRGREPLFVSCTPKGCVELLIRTGVEIAGKNAVVIGRSNIVGL 235

Query: 222 PAALLLQGEDATVSVVHSRTKDPEELTRQADIIISAVGRANMVKGSWIKPGAVIIDVGIN 281
           P +LLLQ  DATVS VH+ TKDPE +TR+ADI+I+A G  N+V+GSW+KPGAV+IDVG  
Sbjct: 236 PMSLLLQRHDATVSTVHAFTKDPEHITRKADIVIAAAGIPNLVRGSWLKPGAVVIDVGTT 295

Query: 282 PVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKR 336
           PVED+S   GYRLVGDVCYEEA  +ASAITPVPGGVGPMTI MLL NTL AAKR
Sbjct: 296 PVEDSSCEFGYRLVGDVCYEEALGVASAITPVPGGVGPMTITMLLCNTLEAAKR 349

BLAST of Chy6G107180 vs. TAIR 10
Match: AT3G12290.1 (Amino acid dehydrogenase family protein )

HSP 1 Score: 391.7 bits (1005), Expect = 5.9e-109
Identity = 187/292 (64.04%), Postives = 233/292 (79.79%), Query Frame = 0

Query: 44  STEATAEVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYVRNKKKA 103
           S++ TA++IDGK +A  IR EI  EV  + +  G VPGLAV++VG RKDS TYV  K+KA
Sbjct: 4   SSDHTAKIIDGKAIAHTIRSEIAEEVRGLSEKHGKVPGLAVVIVGSRKDSQTYVNTKRKA 63

Query: 104 CDSVGIKSFEVNLPEDCTEQEVLKYISDFNDDPSVHGILVQLPLPKHMNEQNILHAVSIE 163
           C  VGIKSF+V LPE+ +E +++  + + N +P VHGILVQLPLPKH+NE++IL A+SI+
Sbjct: 64  CAEVGIKSFDVGLPEEVSEADLISKVHELNSNPDVHGILVQLPLPKHINEEHILGAISID 123

Query: 164 KDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNIGIKGKRAVVIGRSNIVGTPA 223
           KDVDGFHPLNIG+LAM+G EPLF+PCTPKGC+ELL R  + IKG+RAVV+GRSNIVG P 
Sbjct: 124 KDVDGFHPLNIGKLAMKGREPLFLPCTPKGCLELLARSGVKIKGQRAVVVGRSNIVGLPV 183

Query: 224 ALLLQGEDATVSVVHSRTKDPEELTRQADIIISAVGRANMVKGSWIKPGAVIIDVGINPV 283
           +LLL   DATV+ VHS TKDPE + R+ADI+I+A G+A+M+KG+WIKPGA +IDVG N V
Sbjct: 184 SLLLLKADATVTTVHSHTKDPEAIIREADIVIAACGQAHMIKGNWIKPGAAVIDVGTNAV 243

Query: 284 EDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKR 336
            D S   GYRLVGDV + EASK+A  ITPVPGGVGPMT+AMLL NT+  AKR
Sbjct: 244 SDPSKKSGYRLVGDVDFAEASKVAGFITPVPGGVGPMTVAMLLRNTVDGAKR 295

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O652711.5e-14675.49Bifunctional protein FolD 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FO... [more]
O652694.1e-11564.57Bifunctional protein FolD 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FO... [more]
A2RVV71.9e-11267.01Bifunctional protein FolD 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FO... [more]
Q9LHH78.3e-10864.04Bifunctional protein FolD 2 OS=Arabidopsis thaliana OX=3702 GN=FOLD2 PE=2 SV=1[more]
A0LE041.3e-8456.90Bifunctional protein FolD OS=Magnetococcus marinus (strain ATCC BAA-1437 / JCM 1... [more]
Match NameE-valueIdentityDescription
A0A0A0L8Y46.9e-18291.46Methenyltetrahydrofolate cyclohydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G1226... [more]
A0A5A7TZL14.5e-18190.91Methenyltetrahydrofolate cyclohydrolase OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3AV554.5e-18190.91Methenyltetrahydrofolate cyclohydrolase OS=Cucumis melo OX=3656 GN=LOC103483291 ... [more]
A0A6J1GAU15.3e-17488.15Methenyltetrahydrofolate cyclohydrolase OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1IDA15.8e-17387.91Methenyltetrahydrofolate cyclohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111474... [more]
Match NameE-valueIdentityDescription
XP_004134331.14.28e-23391.46bifunctional protein FolD 4, chloroplastic [Cucumis sativus] >KGN56536.1 hypothe... [more]
XP_008438072.14.99e-23290.91PREDICTED: bifunctional protein FolD 4, chloroplastic isoform X1 [Cucumis melo] ... [more]
XP_023528480.16.21e-22689.26bifunctional protein FolD 4, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
KAG6582599.13.59e-22588.98Bifunctional protein FolD 4, chloroplastic, partial [Cucurbita argyrosperma subs... [more]
XP_038879177.11.03e-22488.71bifunctional protein FolD 4, chloroplastic-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT4G00620.11.1e-14775.49Amino acid dehydrogenase family protein [more]
AT4G00600.12.9e-11664.57Amino acid dehydrogenase family protein [more]
AT2G38660.11.4e-11367.01Amino acid dehydrogenase family protein [more]
AT2G38660.31.4e-11367.01Amino acid dehydrogenase family protein [more]
AT3G12290.15.9e-10964.04Amino acid dehydrogenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000672Tetrahydrofolate dehydrogenase/cyclohydrolasePRINTSPR00085THFDHDRGNASEcoord: 310..328
score: 81.78
coord: 156..177
score: 57.69
coord: 203..223
score: 57.69
coord: 80..102
score: 55.69
coord: 252..281
score: 55.9
coord: 293..309
score: 45.7
coord: 121..148
score: 55.63
IPR000672Tetrahydrofolate dehydrogenase/cyclohydrolaseHAMAPMF_01576THF_DHG_CYHcoord: 49..337
score: 38.98748
NoneNo IPR availableGENE3D3.40.50.720coord: 52..313
e-value: 4.0E-116
score: 388.7
NoneNo IPR availableGENE3D3.40.50.10860Leucine Dehydrogenase, chain A, domain 1coord: 54..331
e-value: 4.0E-116
score: 388.7
NoneNo IPR availablePANTHERPTHR48099:SF10BIFUNCTIONAL PROTEIN FOLD 1, MITOCHONDRIALcoord: 34..339
NoneNo IPR availablePANTHERPTHR48099C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATEDcoord: 34..339
NoneNo IPR availableSUPERFAMILY53223Aminoacid dehydrogenase-like, N-terminal domaincoord: 49..168
IPR020630Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domainPFAMPF00763THF_DHG_CYHcoord: 51..167
e-value: 1.4E-42
score: 144.7
IPR020631Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domainPFAMPF02882THF_DHG_CYH_Ccoord: 170..336
e-value: 9.1E-69
score: 229.9
IPR020631Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domainCDDcd01080NAD_bind_m-THF_DH_Cyclohydcoord: 162..334
e-value: 1.77566E-100
score: 291.381
IPR020867Tetrahydrofolate dehydrogenase/cyclohydrolase, conserved sitePROSITEPS00767THF_DHG_CYH_2coord: 314..322
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 169..335

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy6G107180.1Chy6G107180.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035999 tetrahydrofolate interconversion
cellular_component GO:0005829 cytosol
molecular_function GO:0004477 methenyltetrahydrofolate cyclohydrolase activity
molecular_function GO:0004488 methylenetetrahydrofolate dehydrogenase (NADP+) activity
molecular_function GO:0003824 catalytic activity