Chy5G103970 (gene) Cucumber (hystrix) v1

Overview


Name	Chy5G103970
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	tRNA (guanine(37)-N1)-methyltransferase
Location	chrH05: 17636580 .. 17640623 (-)
RNA-Seq Expression	Chy5G103970
Synteny	Chy5G103970

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGGACGAAAGCAAATTCGACGTGCATCTGAATCTCTGGGCCTTAAGAATTCCGAGGGAACTTTGTAAGGCCGCCATTAAAATACTGAACGGGTACGCCATGTTTTGTGTTATATTGTTTCTTTGTTATACTTTGATAGTTATTCGATTTCAATTAATCATTTAGGTAATGGCATTTAGTATTAGTGTTACTCATATGTTGTTCTCGTTGAACTGTCTTTCTCATTTGAGTTCATCATTATTTGAAAACTTATCAGGATTTTCCTTATAATTTTTAGTTTTTTTTCTTTGAAAAGTGTTCTTGTTATTAATTTTTGTTTTAAAGCAGAATTTTCTTTGGCCCTCAGTTCGTTTCTGTTGTAAAATTTTCAGTTACTTAATTGACAAGCCCCGGATTAAACCTGTGACTGAAGATCCAACATGTGATAAGAATCGTTATGTTATATTATCTGAAAAGGTTCAAACTCCAGGTAAAGATCTCCATCTTCGTCTTTATATTTATTCTGTTAACTATATGCTTGGATTGCAATTTCATGTACTTTATGGAAACTCTTCTATAGCACATTCTGTTCGCTGAAGTTCCAATTCTTGTTCATTGATTTTTTTCATGTTACCTTAGTGGCTTTTTGGCTCTCTTCATGCACTCAAGTTCTTAAATAGTACTAAAATTATTAAGCGAGTAATTTTTTTAATCTTAAAACATTAATGTTTGTAGGGTATAACCATCAACTTGAATGCATCTTAAGTTAGAAAATTGTCCAAAAACCGCCTAGATATCAAATGAAACCAGCTTTAAAAAATCTAAATATCGAGAAAAGAAACTAAATTAACATCGTGTAACTAAGTAATTTTCAGAAAAATAGTCTCGTTTCCCTTTAGAAATATTAATCACTGTTTTCTCTTGTTTTCTCGATGGAATTTTCCTCGATCATGTCCTTAGATGACACCATTTTTCTATCTTGTTGATTCTTTGAATGCTCAGAATTATCAGAAATTCCAGAAATGAAGGTTGAAGAATTGAAAGGCTTATGCATGTATGAACGAGTTCCCTATTCACTAACCCTTGGATATTCATATTGGGGAGCAGGTATTTTCAATAATTCTACCCTTATCACACATTGTGCAAATTTTTTTCACCTTAGGATTGCCTGTTGAACTCCAGATATTTATAGATGCCTTTAACTTTCTAATTGCAAGTCTGAAGTGTTATGTAAGGCATATCTAGCACCATTTTTAATGATCCCATTCCCATTACTCAAAACCCAAACGTTTTCTGCATTGTGACTGTCCACATGCTGATAGAATCTGGTTGTCTTTGCCTCTCATTTGATATTATAGCAACTACATGTGATGTGACTGTTCTACTTCTACATCCAGTGGTTGCAATTATAAGAACAATGTTCGATTTCTGAAAGATAGTTTAAACAATTCTGTGAGCGAATTTACCTGTCATTTGATGTGTTTGTATTGTACATACTCGACTAACTTACTGTGTGGTTGCTCTGTAGATCATATTCTCAAGAAGATTTTGCCTCCTGAAGTGGAAGTGCCTTCATCTTTTGAGACAATAGTTAAGTACTCTAACCCCTGCCATGTTCCACCTACGTTTTCCTTTCTACATTTTGTATCCATTTCCATATGCTCTGTATAGCTTTTATGTGCGTTCTTTTGTTGCTCTGTATTGCATTTTGGTACCACTTGTTTATGTGACATTGGTTGTAACGTTGCTTTTTCATGTACAACTCTTGACAGTGAGGTAATTTTGTACTTTGATTTGCAGGTCACGTTGCTCACCTGAATATACATGACGAATTGCTTCCATATAAGGATGTCATTGCGAAGGTTATTTATGATGTATGTCATCAGTATCTTTTTACTCATTTTCCTAATTATTTTGAAATGTTCATTGTGATTTATTGATCACTATAATTTAGCTGTTACTTCATTTTTTTATTCTTAATTTTGTTTCTCTCCCCTTCTTTTCCTCCATGCATATGTTGGGGGTACCTTTTTAAGAAATTCAAATGTTCAGAATCGGATATTTAATATTTTTATCCTCCCCCTCCATCCAGCAATATGCACACACACACATTTTCCATATCGAAACTCTTCTGTAACTATTTCTTTTTGTAATCTTTGCAGAAAAATTATCCGAGGATTAAAACAGTTGTAAATAAAGTGGGTTCTATAACAAATGAGTTTCGGGTTCCAAAGTTTGAAATACTGAAGGGAGAGAATGATATGGTAACAGAAGTAAAGCAATATGGGGCGACTTTTAAGCTCGACTACAGTTTGGTCTACTGGAACTCAAGATTGGAACATGAACACATAAGGTTGGTTTCACTCTTCCAACCAGGAGAGGTAATTTGTGACATGTTTGCTGGTATTGGTCCTTTTGCAATTCCAGCCGCCCAGAAAGAATGTATAGTATATGCCAATGACTTGAATCCAGACAGCGTCCGATATCTGAAGGTTAATGCAGAAATCAATAAAGTTAGTGGTCGCATTCATGTATATAATTTGGATGCTAGGAAGTTTATTTCTCAATTGATGATAGTTCCCCCTAATGAAAGTAATTCAGAATCTGTCACCTCAATACTAAAAGCTTGTGAGAAAGGCATTGGAGGGACCAATCAAGAATCACCATCGGCAAATGGTATGCTGACTGGTATTCTCTTATTATCATTTAAAGAAAATTTCACATCGTCTTCAGGAGATTAGTGTATATGATCTTAATTTTCTTTCTTGCTTCAGTTCAGGTGCAAGAAATTCAAGATGATATTAAAGTTAGCAATAAATCTGTAGAACATTCAAGGAGTGCTGATATATCCGTTGCTGTTCTTAAAAGATCCTCAGAAAGTTGTGAGAACGAGAAAGGTAACATACTCTACCATAGCTTGTGATTGAACAACCAAGACCATTTCTTCCTATAATTTTCTTTGCAGATTTTTTTTTAATGAAAATTTGCTTTGCAGACAATGGAATTGCTGATGATGCTTCCACTCGTTTAGCCGGTAGGAGAAATGGAAACGGAAATAAGAGGATGAAGGGGTCCAGTGTATCTCATGCTAGGACCTGGGAACACGTTGATCATGTGATTATGAACCTCCCTGCTTCTGCTTTACATTTTCTTGGTACGAATGACCTTTTTTCTTTTAAATTTCATTTCTCTTATTGCAGTTTTAGCATATTTCTCTACATTTGTGTGTCAGAATGAAGAGTTAAAAGTTGGGGAAATAACAATGTTTGGAAAGGGAAACAATTACTTTTTAATGCTTTAGATATGAAGGGAAACCATAAACGATTTTTTATCTCTTACTATCACCATGACAGAGATATTTTGGATATGTCATATTTCCTAATTCCTACCTTTCAAATGGAATTCTTTGTTGTAAGTTTTGTAGTCACTCTCTATACCAATTTTATGAGTTTCTTCAATCTCCTTTAGCTCTTGAGTGAGGGAGAATGCCTTATCTCCTCCACTCAATTATCTCAACTCGTCTTTTGTATCCATATCTATATTTTGTTTAGTTAACCAATGAAGGATATTTTGTTATTTTCTTGCCAGATGCGTTTAGAGGCTCAATTAAGAAGAAATACTGGAAGGGCTCCCTTCCTTGGATTCACTGTTACTGCTTCATGCGAGCAAATGAAAACCAAGATTTTATCATTTCGGTATGTTTATACTAGATTACTACAGATCTTTATCTGTCTTTGTCTTTGTCTTCTTTTTGATAATTGTATTTCTTTCTCACCATTCTATTTGTGGATATTTGTCTTGAAAAAATTGTCTTTCTTCTCATACCATCCTCTTTTCACTGTTTCACTCTAGGAGGCTGAGTTTGCCTTGAATGCTAATATTCAAGACCCCATTTTTCATAGGGTCAGAGATGTTGCACCAAACAAGGTATTGCTTTGTGTGTGTGTATAAATGGCAACTACTTGTTTTTAACTCATTTTTCATTTTTGTTTTTATCAGGCTATGTACTGTTTGAGCTTTAGGCTTCCGGAACAATGCGTCAATGAAGACACGACTAGCTAA

mRNA sequence

ATGTTGGACGAAAGCAAATTCGACGTGCATCTGAATCTCTGGGCCTTAAGAATTCCGAGGGAACTTTGTAAGGCCGCCATTAAAATACTGAACGGTTACTTAATTGACAAGCCCCGGATTAAACCTGTGACTGAAGATCCAACATGTGATAAGAATCGTTATGTTATATTATCTGAAAAGGTTCAAACTCCAGAATTATCAGAAATTCCAGAAATGAAGGTTGAAGAATTGAAAGGCTTATGCATGTATGAACGAGTTCCCTATTCACTAACCCTTGGATATTCATATTGGGGAGCAGGTCACGTTGCTCACCTGAATATACATGACGAATTGCTTCCATATAAGGATGTCATTGCGAAGGTTATTTATGATAAAAATTATCCGAGGATTAAAACAGTTGTAAATAAAGTGGGTTCTATAACAAATGAGTTTCGGGTTCCAAAGTTTGAAATACTGAAGGGAGAGAATGATATGGTAACAGAAGTAAAGCAATATGGGGCGACTTTTAAGCTCGACTACAGTTTGGTCTACTGGAACTCAAGATTGGAACATGAACACATAAGGTTGGTTTCACTCTTCCAACCAGGAGAGGTAATTTGTGACATGTTTGCTGGTATTGGTCCTTTTGCAATTCCAGCCGCCCAGAAAGAATGTATAGTATATGCCAATGACTTGAATCCAGACAGCGTCCGATATCTGAAGGTTAATGCAGAAATCAATAAAGTTAGTGGTCGCATTCATGTATATAATTTGGATGCTAGGAAGTTTATTTCTCAATTGATGATAGTTCCCCCTAATGAAAGTAATTCAGAATCTGTCACCTCAATACTAAAAGCTTGTGAGAAAGGCATTGGAGGGACCAATCAAGAATCACCATCGGCAAATGTTCAGGTGCAAGAAATTCAAGATGATATTAAAGTTAGCAATAAATCTGTAGAACATTCAAGGAGTGCTGATATATCCGTTGCTGTTCTTAAAAGATCCTCAGAAAGTTGTGAGAACGAGAAAGACAATGGAATTGCTGATGATGCTTCCACTCGTTTAGCCGGTAGGAGAAATGGAAACGGAAATAAGAGGATGAAGGGGTCCAGTGTATCTCATGCTAGGACCTGGGAACACGTTGATCATGTGATTATGAACCTCCCTGCTTCTGCTTTACATTTTCTTGATGCGTTTAGAGGCTCAATTAAGAAGAAATACTGGAAGGGCTCCCTTCCTTGGATTCACTGTTACTGCTTCATGCGAGCAAATGAAAACCAAGATTTTATCATTTCGGAGGCTGAGTTTGCCTTGAATGCTAATATTCAAGACCCCATTTTTCATAGGGTCAGAGATGTTGCACCAAACAAGGCTATGTACTGTTTGAGCTTTAGGCTTCCGGAACAATGCGTCAATGAAGACACGACTAGCTAA

Coding sequence (CDS)

ATGTTGGACGAAAGCAAATTCGACGTGCATCTGAATCTCTGGGCCTTAAGAATTCCGAGGGAACTTTGTAAGGCCGCCATTAAAATACTGAACGGTTACTTAATTGACAAGCCCCGGATTAAACCTGTGACTGAAGATCCAACATGTGATAAGAATCGTTATGTTATATTATCTGAAAAGGTTCAAACTCCAGAATTATCAGAAATTCCAGAAATGAAGGTTGAAGAATTGAAAGGCTTATGCATGTATGAACGAGTTCCCTATTCACTAACCCTTGGATATTCATATTGGGGAGCAGGTCACGTTGCTCACCTGAATATACATGACGAATTGCTTCCATATAAGGATGTCATTGCGAAGGTTATTTATGATAAAAATTATCCGAGGATTAAAACAGTTGTAAATAAAGTGGGTTCTATAACAAATGAGTTTCGGGTTCCAAAGTTTGAAATACTGAAGGGAGAGAATGATATGGTAACAGAAGTAAAGCAATATGGGGCGACTTTTAAGCTCGACTACAGTTTGGTCTACTGGAACTCAAGATTGGAACATGAACACATAAGGTTGGTTTCACTCTTCCAACCAGGAGAGGTAATTTGTGACATGTTTGCTGGTATTGGTCCTTTTGCAATTCCAGCCGCCCAGAAAGAATGTATAGTATATGCCAATGACTTGAATCCAGACAGCGTCCGATATCTGAAGGTTAATGCAGAAATCAATAAAGTTAGTGGTCGCATTCATGTATATAATTTGGATGCTAGGAAGTTTATTTCTCAATTGATGATAGTTCCCCCTAATGAAAGTAATTCAGAATCTGTCACCTCAATACTAAAAGCTTGTGAGAAAGGCATTGGAGGGACCAATCAAGAATCACCATCGGCAAATGTTCAGGTGCAAGAAATTCAAGATGATATTAAAGTTAGCAATAAATCTGTAGAACATTCAAGGAGTGCTGATATATCCGTTGCTGTTCTTAAAAGATCCTCAGAAAGTTGTGAGAACGAGAAAGACAATGGAATTGCTGATGATGCTTCCACTCGTTTAGCCGGTAGGAGAAATGGAAACGGAAATAAGAGGATGAAGGGGTCCAGTGTATCTCATGCTAGGACCTGGGAACACGTTGATCATGTGATTATGAACCTCCCTGCTTCTGCTTTACATTTTCTTGATGCGTTTAGAGGCTCAATTAAGAAGAAATACTGGAAGGGCTCCCTTCCTTGGATTCACTGTTACTGCTTCATGCGAGCAAATGAAAACCAAGATTTTATCATTTCGGAGGCTGAGTTTGCCTTGAATGCTAATATTCAAGACCCCATTTTTCATAGGGTCAGAGATGTTGCACCAAACAAGGCTATGTACTGTTTGAGCTTTAGGCTTCCGGAACAATGCGTCAATGAAGACACGACTAGCTAA

Protein sequence

MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEKVQTPELSEIPEMKVEELKGLCMYERVPYSLTLGYSYWGAGHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLNPDSVRYLKVNAEINKVSGRIHVYNLDARKFISQLMIVPPNESNSESVTSILKACEKGIGGTNQESPSANVQVQEIQDDIKVSNKSVEHSRSADISVAVLKRSSESCENEKDNGIADDASTRLAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAEFALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNEDTTS*

Homology

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BLAST of Chy5G103970 vs. ExPASy Swiss-Prot
Match: F6HH45 (tRNA (guanine(37)-N1)-methyltransferase 2 OS=Vitis vinifera OX=29760 GN=VIT_11s0016g04350 PE=3 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 1.3e-180
Identity = 325/501 (64.87%), Postives = 382/501 (76.25%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           +LDESKFD+ L LWALRIP +LCK A +ILNGYL+DKPR+KP+TEDPTC+ NRY+ILSE+
Sbjct: 2   LLDESKFDLQLKLWALRIPSQLCKVASRILNGYLLDKPRVKPITEDPTCETNRYIILSER 61

Query: 61  VQTPELSEIPEMKVEELKGLCMYERVPYSLTLGYSYWGAGHV------------------ 120
           ++ P+LS+IPE K +ELK LC  E VPYSLTLGYSYW A HV                  
Sbjct: 62  IKNPDLSDIPEDKRDELKVLCKIEVVPYSLTLGYSYWSADHVLKQILPDGVEVPSSFETI 121

Query: 121 -------AHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGE 180
                  AHLNI  ELLPYKDVIAKVIYDKNYPRIKTVVNKVG+ITNEFRVPKFEIL G+
Sbjct: 122 TFSLCHIAHLNITGELLPYKDVIAKVIYDKNYPRIKTVVNKVGTITNEFRVPKFEILVGK 181

Query: 181 NDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQ 240
           +DMVTEVKQY ATFKLDYSLVYWNSRLEHEH+RLVS F+PG++ICDMF+G+GPFAIPAAQ
Sbjct: 182 DDMVTEVKQYRATFKLDYSLVYWNSRLEHEHMRLVSQFRPGQIICDMFSGVGPFAIPAAQ 241

Query: 241 KECIVYANDLNPDSVRYLKVNAEINKVSGRIHVYNLDARKFISQLMIVPPNESNSESVTS 300
           K C+VYANDLNPDS+RYLK+NA+INKV   I  YN+DARKFISQLM VP +E   E    
Sbjct: 242 KGCLVYANDLNPDSIRYLKINAKINKVDDNIWAYNMDARKFISQLMEVPVHEILPEPDVP 301

Query: 301 ILKACEKGIGGTNQESPSAN----VQVQEIQDDIKVSNKSVEHSRS-ADISVAVLKRSSE 360
           +LKA E+     N ++ S N    V+   +  D   +   +E S + AD +VA +K+ S 
Sbjct: 302 VLKATEECSIQANVQTESQNGGLTVEAIGVHSDDSSNMDGIEGSCTVADDTVAAVKKHSG 361

Query: 361 SCENEKDNGIADDASTRLAGRRNGNGNKRMKGS-SVSHARTWEHVDHVIMNLPASALHFL 420
           SCE E  NG  D+A+  + GRR    NKRM+GS S S+ + WEHVDHVIMNLPASA+ FL
Sbjct: 362 SCEEE--NGTYDNATVSVVGRRKDRMNKRMRGSESSSNIKPWEHVDHVIMNLPASAIQFL 421

Query: 421 DAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAEFALNANIQDPIFHRVRDVAPN 470
           DAFRG I++KYWKGSLPWIHCYCF+RANE  + IISEAE ALNA+IQ+PI HRVRDVAPN
Sbjct: 422 DAFRGLIQRKYWKGSLPWIHCYCFIRANETNEMIISEAETALNASIQEPILHRVRDVAPN 481

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BLAST of Chy5G103970 vs. ExPASy Swiss-Prot
Match: Q93YU6 (tRNA (guanine(37)-N1)-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=At3g56120 PE=2 SV=1)

HSP 1 Score: 580.5 bits (1495), Expect = 1.7e-164
Identity = 293/488 (60.04%), Postives = 356/488 (72.95%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           M DESKFDV+L LWALRIPRELCK+A +ILNGY+++ PRIKP+TEDPTC+K R VILSE 
Sbjct: 1   MFDESKFDVNLKLWALRIPRELCKSASRILNGYMLNMPRIKPITEDPTCEKTRLVILSES 60

Query: 61  VQTPELSEIPEMKVEELKGLCMYERVPYSLTLGYSYWGA--------------------- 120
           V+  +LSEIPE K+ +LK L   E VP+S+TLGYSYW A                     
Sbjct: 61  VKNADLSEIPEEKLNQLKKLSELEVVPHSVTLGYSYWSADHLLKQILPDGLDIPSSFETI 120

Query: 121 GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180
           GH+AHLN+HDELLP+KDVIAKVIYDKNYPRIKT+VNKVG+I+NEFRVPKFE+L GEN M 
Sbjct: 121 GHIAHLNLHDELLPFKDVIAKVIYDKNYPRIKTIVNKVGTISNEFRVPKFEVLAGENGME 180

Query: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240
           TEVKQYGA FKLDY LVYWNSRLEHEH+RL SLF+PGE +CDMFAGIGPFAIPAAQK C 
Sbjct: 181 TEVKQYGARFKLDYGLVYWNSRLEHEHMRLSSLFKPGETVCDMFAGIGPFAIPAAQKGCF 240

Query: 241 VYANDLNPDSVRYLKVNAEINKVSGRIHVYNLDARKFISQLMIVPPNESNSESVTSILKA 300
           VYANDLNPDSVRYLK+NA+ NKV   I V+N+DARKF S LM V   E N +SV    K 
Sbjct: 241 VYANDLNPDSVRYLKINAKFNKVDDLICVHNMDARKFFSHLMAVSTCEDNLQSVADNDKT 300

Query: 301 CEKGIGGTNQESPSANVQVQEIQDDIKVSNKSVEHSRSADISVAVLKRSSESCENEKDNG 360
            E  +    + + S         ++I+ SN S+     A+      K+ S + + E  NG
Sbjct: 301 KEAAVSRGGETNSSG--------EEIRESNASINEPLGAN------KKPSGTTKTE--NG 360

Query: 361 IADDASTRLAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIKKK 420
           +  D  +       G+ NKR++ + +  A+ WEH+DHVIMNLPASAL FLD+F   I+KK
Sbjct: 361 VGKDCKS-----IEGHANKRLRQTLLPIAKPWEHIDHVIMNLPASALQFLDSFSNVIQKK 420

Query: 421 YWKGSLPWIHCYCFMRANENQDFIISEAEFALNANIQDPIFHRVRDVAPNKAMYCLSFRL 468
           YWKG LP IHCYCF+RA+E  +FII+EAE AL  +I+DP+FH+VRDVAPNKAM+CLSFRL
Sbjct: 421 YWKGPLPLIHCYCFIRASETTEFIIAEAETALKFHIEDPVFHKVRDVAPNKAMFCLSFRL 467

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BLAST of Chy5G103970 vs. ExPASy Swiss-Prot
Match: C5XX79 (tRNA (guanine(37)-N1)-methyltransferase OS=Sorghum bicolor OX=4558 GN=Sb04g025390 PE=3 SV=1)

HSP 1 Score: 570.1 bits (1468), Expect = 2.3e-161
Identity = 287/467 (61.46%), Postives = 355/467 (76.02%), Query Frame = 0


Query: 2   LDESKFDVHLNLWALRIPRELCKAAIKIL-NGYLIDKPRIKPVTEDPTCDKNRYVILSEK 61
           LDESKF+  L LWALRIPREL  A  ++L +GYL+DKPR+K V EDP  DKNR VILSEK
Sbjct: 4   LDESKFEQRLQLWALRIPRELSSAVTRLLRSGYLLDKPRVKTVVEDPEGDKNRLVILSEK 63

Query: 62  VQTPELSEIPEMKVEELKGLCMYERVPYSLTLGYSYWGAGHVAHLNIHDELLPYKDVIAK 121
           +Q P+LS++P+ +++ LK LC  + VPY+LTLGYSYW AGHVAHLNI D+LLPYK+VIAK
Sbjct: 64  IQKPDLSDMPQQELDSLKQLCNVDVVPYTLTLGYSYWSAGHVAHLNISDDLLPYKNVIAK 123

Query: 122 VIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKLDYSLVYWNS 181
           VIYDKNYPRI+TV NKVG+ITNEFRVPKFEIL G+NDMVTEVKQYGATFKLDY LVYWNS
Sbjct: 124 VIYDKNYPRIQTVANKVGTITNEFRVPKFEILAGKNDMVTEVKQYGATFKLDYGLVYWNS 183

Query: 182 RLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLNPDSVRYLKVNAEIN 241
           RL+HEHIRLVSLF+ G+VICDMFAGIGPFAIPAAQK C+VYANDLNPDSV YL+ NA+IN
Sbjct: 184 RLDHEHIRLVSLFKKGDVICDMFAGIGPFAIPAAQKGCVVYANDLNPDSVHYLRTNAKIN 243

Query: 242 KVSGRIHVYNLDARKFISQLMIVPPNESNSESVTSILKACEKGIGGTNQESPSANVQVQE 301
           KV   I  YN+DAR F+  LM VP  E+ S+   +  ++  K  G    E+ S+N    +
Sbjct: 244 KVDDYIFAYNMDARVFMQNLMTVPGLETGSDCQVAADESYPKE-GVPANENSSSNGNHND 303

Query: 302 IQDDIKVSNKSVEHSRSADISVAVLKRSSESCENEKDNGIADDASTRLAGRRNGNGNKRM 361
           +++    S      S  A  +    +++SE CE++  +G  D + T+   RRN   NKR+
Sbjct: 304 VREG---SQNGANESSVASTTAKKRQQTSEECESDCQDG--DASQTK---RRN---NKRV 363

Query: 362 KGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIKKKYWKGSLPWIHCYCFMRANENQ 421
           +G     ++ WEH DHV+MNLPASAL FLD F G ++KKYW GSLPWIHCYCF+R++E++
Sbjct: 364 RGPGPPPSKPWEHFDHVLMNLPASALQFLDCFDGLVQKKYWTGSLPWIHCYCFIRSSESE 423

Query: 422 DFIISEAEFALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNED 468
           + I+S     LNA I +PIFHRVRDVAPNKAM+CLSF+LP +C+ ED
Sbjct: 424 ESILSN---KLNAKIAEPIFHRVRDVAPNKAMFCLSFKLPMECLRED 455

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BLAST of Chy5G103970 vs. ExPASy Swiss-Prot
Match: A9T6G5 (tRNA (guanine(37)-N1)-methyltransferase OS=Physcomitrium patens OX=3218 GN=PHYPADRAFT_141055 PE=3 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 4.5e-104
Identity = 210/472 (44.49%), Postives = 277/472 (58.69%), Query Frame = 0


Query: 2   LDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEKV 61
           +D+S F V L   ALRIP++       +L+ YL+D PR+K + EDP     R ++ SE +
Sbjct: 11  IDKSAFVVRLPRVALRIPKQKSHRVASLLSKYLLDMPRVKHIVEDPQDPSMRLLLFSEAI 70

Query: 62  QTPELSEIPEMKVEELKGLCMYERVPYSLTLGYSYWGA---------------------G 121
             P+L  + E K+  +K +   + V + + L YSYW                       G
Sbjct: 71  AQPDLEGVSEEKILAVKEIVPLDVVQHEVVLDYSYWPVEHILKEILPAGCEVPSSFETIG 130

Query: 122 HVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVT 181
           H+AHLN+ ++LL YK +IA+VI DKN P++KTVVNKVG+ITNEFRVP+FEIL GE  +VT
Sbjct: 131 HIAHLNLREDLLTYKKIIAEVILDKN-PKLKTVVNKVGTITNEFRVPEFEILAGEPSLVT 190

Query: 182 EVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIV 241
           E+KQ+GATF+LDY +VYWNSRLE EH RL + F+PG+VI DMFAGIGPFAIPAAQ+ C V
Sbjct: 191 EIKQHGATFRLDYGMVYWNSRLEGEHKRLFAQFKPGQVIVDMFAGIGPFAIPAAQQGCAV 250

Query: 242 YANDLNPDSVRYLKVNAEINKVSGRIHVYNLDARKFISQLMIVPPNESNSESVTSILKAC 301
           YANDLNP SV++LK+N++INKV   I  +NLDAR+F+ +L  V   E     + S     
Sbjct: 251 YANDLNPTSVKFLKLNSDINKVGESIKAFNLDAREFMRKL--VTEEEGCVGEIASSTLIA 310

Query: 302 EKGIGGTNQESPSANVQVQEIQDDIKVSNKSVEH-SRSADISVAVLKRSSESCENEKDNG 361
           E  + GT   S   N        D+  S K  E  ++ A +S  V               
Sbjct: 311 ENSLNGTRIPSSKENSSSLTEGSDMPSSKKPEERKAKKAKVSEVV--------------- 370

Query: 362 IADDASTRLAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIKKK 421
                              +MK   +S  + WEH DHV+MNLPASAL FLD   G + K 
Sbjct: 371 -------------------KMK---ISDVKPWEHFDHVVMNLPASALEFLDVLNGLLCKG 430

Query: 422 YWKGSLPWIHCYCFMRANENQDFIISEAEFALNANIQDPIFHRVRDVAPNKA 452
            WKG++P +HCYCFMR+NE    I+ +AE  L  +I DP  + VRDVAPNKA
Sbjct: 431 RWKGTMPRVHCYCFMRSNETNADIVKKAETYLGGSIADPDVYVVRDVAPNKA 442

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BLAST of Chy5G103970 vs. ExPASy Swiss-Prot
Match: F4NUJ6 (tRNA (guanine(37)-N1)-methyltransferase OS=Batrachochytrium dendrobatidis (strain JAM81 / FGSC 10211) OX=684364 GN=TRM5 PE=3 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 8.3e-66
Identity = 160/486 (32.92%), Postives = 248/486 (51.03%), Query Frame = 0


Query: 2   LDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEKV 61
           L+ + F+    L AL+IP      A++ L  +++  PR++ + +DPT  K R ++L   +
Sbjct: 16  LNHALFESSHTLPALKIPAHASGIAMETLRSHILVAPRLRTIVDDPTDKKWRLLLLDPSM 75

Query: 62  QTPELSEIPEMKVEELKGLCMYERVPYSLTLGYSYW-----------------GA----G 121
           +  E++++P   ++E       + V +++ L Y YW                 GA    G
Sbjct: 76  EASEINDLPN-PLKEFALKHEAKLVKHTIELKYDYWTSDQVLRSILPDEMETPGAFETVG 135

Query: 122 HVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVT 181
           H+AHLN+ D   P+K +I +VI DK+   IKTVVNK+ +I + FR  + EIL G NDM  
Sbjct: 136 HIAHLNLRDRYQPFKHIIGQVILDKS-SHIKTVVNKLDNIDHTFRFFQMEILAGINDMNA 195

Query: 182 EVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQ-KECI 241
           ++K+ G  F  D+S VYWNSRL+ EH R++ LF   ++ICD+FAG+GPFA+PAA+ K C+
Sbjct: 196 KLKEGGCFFHFDFSKVYWNSRLQGEHDRIIKLFGQNDLICDVFAGVGPFALPAAKHKRCV 255

Query: 242 VYANDLNPDSVRYLKVNAEINKVSGRIHVYNLDARKFISQLMIVPPNESNSESVTSILKA 301
           V+ANDLNP S +YL  N ++NK+  RI  +N+D R+FI Q +     + N+ ++ + +  
Sbjct: 256 VFANDLNPQSFKYLMENIKLNKLETRILPFNMDGRQFIKQSL----EDLNNPAIWNKI-- 315

Query: 302 CEKGIGGTNQESPSANVQVQEIQDDIKVSNKSVEHSRSADISVAVLKRSSESCENEKDNG 361
                    ++ P++N          K  N+ VE    A ++           +    +G
Sbjct: 316 --------TKQKPTSN---------DKKRNRKVESPTVAPLT-----------DQPAISG 375

Query: 362 IADDASTRLAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRG---SI 421
           I                                H  H +MNLPA+A+ FLDAF G    +
Sbjct: 376 I-------------------------------RHFKHYVMNLPATAIEFLDAFHGLYSGM 434

Query: 422 KKKYWKGSLPWIHCYCFMRANENQDFIISEAEFALNANIQDPIF--HRVRDVAPNKAMYC 461
           +       LP IHC+CF  A + +  +I   E  +   +   +   H VR VAPNK M C
Sbjct: 436 RDVIMDSDLPTIHCHCFSNAKDVKADVIERVERVIGMPLGSNLIMVHSVRTVAPNKDMLC 434

[Close]

BLAST of Chy5G103970 vs. ExPASy TrEMBL
Match: A0A0A0LFW8 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G005350 PE=3 SV=1)

HSP 1 Score: 921.4 bits (2380), Expect = 1.5e-264
Identity = 457/493 (92.70%), Postives = 465/493 (94.32%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKVEELKGLCMYERVPYSLTLGYSYWGAG-------------------- 120
           VQTPELSEIPEMK+EELKGLCM+ERVPYSLTLGYSYWGA                     
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 ---HVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 180
              HVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND
Sbjct: 121 LLCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 180

Query: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 240
           MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE
Sbjct: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 240

Query: 241 CIVYANDLNPDSVRYLKVNAEINKVSGRIHVYNLDARKFISQLMIVPPNESNSESVTSIL 300
           CIVYANDLNPDS+RYLKVNAEINKVSGR+HVYNLDARKFISQLMIVPPNESNSESVTSIL
Sbjct: 241 CIVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL 300

Query: 301 KACEKGIGGTNQESPSANVQVQEIQDDIKVSNKSVEHSRSADISVAVLKRSSESCENEKD 360
           KACEKGIGGTNQESP ANVQVQE+QD IK+SNKSVE SRSADISVAVLKRSSESCENEKD
Sbjct: 301 KACEKGIGGTNQESPLANVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENEKD 360

Query: 361 NGIADDASTRLAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIK 420
           NGIADDASTR+AGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIK
Sbjct: 361 NGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIK 420

Query: 421 KKYWKGSLPWIHCYCFMRANENQDFIISEAEFALNANIQDPIFHRVRDVAPNKAMYCLSF 471
           KKYWKGSLPWIHCYCFMRANENQDFIISEAE ALNANIQDPIFHRVRDVAPNKAMYCLSF
Sbjct: 421 KKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSF 480

[Close]

BLAST of Chy5G103970 vs. ExPASy TrEMBL
Match: A0A5D3CFY1 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G00100 PE=3 SV=1)

HSP 1 Score: 913.3 bits (2359), Expect = 4.2e-262
Identity = 450/471 (95.54%), Postives = 463/471 (98.30%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKVEELKGLCMYERVPYSLTLGYSYWGAGHVAHLNIHDELLPYKDVIAK 120
           VQTPELSEIPEMK+EELKGLCM+ERVPYSLTLGYSYWGAGHVAHLNIHDELLPYKDVIAK
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGAGHVAHLNIHDELLPYKDVIAK 120

Query: 121 VIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKLDYSLVYWNS 180
           VIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKLDYSLVYWNS
Sbjct: 121 VIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKLDYSLVYWNS 180

Query: 181 RLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLNPDSVRYLKVNAEIN 240
           RLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLNPDSVRYLKVNAEIN
Sbjct: 181 RLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLNPDSVRYLKVNAEIN 240

Query: 241 KVSGRIHVYNLDARKFISQLMIVPPNESNSESVTSILKACEKGIGGTNQESPSANVQVQE 300
           KVSGR+HVYNLDARKFISQLMIVPPNESNSE+VTSILKA E+GIGGTNQESP ANVQVQE
Sbjct: 241 KVSGRVHVYNLDARKFISQLMIVPPNESNSETVTSILKASEEGIGGTNQESPLANVQVQE 300

Query: 301 IQDDIKVSNKSVEHSRSADISVAVLKRSSESCENEKDNGIADDASTRLAGRRNGNGNKRM 360
           +Q DI++SNK VEHSRSADISVAVLKRSSESCENEK++ IADDASTR+AGRRNGNGNKRM
Sbjct: 301 VQ-DIEISNKFVEHSRSADISVAVLKRSSESCENEKEDRIADDASTRVAGRRNGNGNKRM 360

Query: 361 KGS-SVSHARTWEHVDHVIMNLPASALHFLDAFRGSIKKKYWKGSLPWIHCYCFMRANEN 420
           KGS +VSHARTWEH+DHVIMNLPASALHFLDAFRGSIKKKYWKGSLPWIHCYCFMRANEN
Sbjct: 361 KGSTNVSHARTWEHIDHVIMNLPASALHFLDAFRGSIKKKYWKGSLPWIHCYCFMRANEN 420

Query: 421 QDFIISEAEFALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNEDTTS 471
           QDFIIS AE ALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNEDTTS
Sbjct: 421 QDFIISVAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNEDTTS 470

[Close]

BLAST of Chy5G103970 vs. ExPASy TrEMBL
Match: A0A1S3C7L3 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103497610 PE=3 SV=1)

HSP 1 Score: 901.0 bits (2327), Expect = 2.1e-258
Identity = 450/492 (91.46%), Postives = 463/492 (94.11%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKVEELKGLCMYERVPYSLTLGYSYWGA--------------------- 120
           VQTPELSEIPEMK+EELKGLCM+ERVPYSLTLGYSYWGA                     
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180
           GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV
Sbjct: 121 GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180

Query: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240
           TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI
Sbjct: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240

Query: 241 VYANDLNPDSVRYLKVNAEINKVSGRIHVYNLDARKFISQLMIVPPNESNSESVTSILKA 300
           VYANDLNPDSVRYLKVNAEINKVSGR+HVYNLDARKFIS+LMIVPPNESNSE+VTSILKA
Sbjct: 241 VYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISRLMIVPPNESNSETVTSILKA 300

Query: 301 CEKGIGGTNQESPSANVQVQEIQDDIKVSNKSVEHSRSADISVAVLKRSSESCENEKDNG 360
            E+GIGGTNQESP ANVQVQE+Q DI++SNK VEHSRSADISVAVLKRSSESCENEKD+ 
Sbjct: 301 SEEGIGGTNQESPLANVQVQEVQ-DIEISNKFVEHSRSADISVAVLKRSSESCENEKDDR 360

Query: 361 IADDASTRLAGRRNGNGNKRMKGS-SVSHARTWEHVDHVIMNLPASALHFLDAFRGSIKK 420
           IADDASTR+AGRRNGNGNKRMKGS +VSHARTWEH+DHVIMNLPASALHFLDAFRGSIKK
Sbjct: 361 IADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAFRGSIKK 420

Query: 421 KYWKGSLPWIHCYCFMRANENQDFIISEAEFALNANIQDPIFHRVRDVAPNKAMYCLSFR 471
           KYWKGSLPWIHCYCFMRANENQDFIIS AE ALNANIQDPIFHRVRDVAPNKAMYCLSFR
Sbjct: 421 KYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAMYCLSFR 480

[Close]

BLAST of Chy5G103970 vs. ExPASy TrEMBL
Match: A0A5A7V0B1 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold41G001350 PE=3 SV=1)

HSP 1 Score: 899.0 bits (2322), Expect = 8.1e-258
Identity = 451/501 (90.02%), Postives = 463/501 (92.42%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKVEELKGLCMYERVPYSLTLGYSYWG---------------------- 120
           VQTPELSEIPEMK+EELKGLCM+ERVPYSLTLGYSYWG                      
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 --------AGHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFE 180
                   AGHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFE
Sbjct: 121 GDNFVLCFAGHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFE 180

Query: 181 ILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFA 240
           ILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFA
Sbjct: 181 ILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFA 240

Query: 241 IPAAQKECIVYANDLNPDSVRYLKVNAEINKVSGRIHVYNLDARKFISQLMIVPPNESNS 300
           IPAAQKECIVYANDLNPDSVRYLKVNAEINKVSGR+HVYNLDARKFISQLMIVPPNESNS
Sbjct: 241 IPAAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNS 300

Query: 301 ESVTSILKACEKGIGGTNQESPSANVQVQEIQDDIKVSNKSVEHSRSADISVAVLKRSSE 360
           E+VTSILKA E+GIGGTNQESP ANVQVQE+Q DI++SNK VEHSRSADISVAVLKRSSE
Sbjct: 301 ETVTSILKASEEGIGGTNQESPLANVQVQEVQ-DIEISNKFVEHSRSADISVAVLKRSSE 360

Query: 361 SCENEKDNGIADDASTRLAGRRNGNGNKRMKGS-SVSHARTWEHVDHVIMNLPASALHFL 420
           SCENEKD+ IADDASTR+AGRRNGNGNKRMKGS +VSHARTWEH+DHVIMNLPASALHFL
Sbjct: 361 SCENEKDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFL 420

Query: 421 DAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAEFALNANIQDPIFHRVRDVAPN 471
           DAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIIS AE ALNANIQDPIFHRVRDVAPN
Sbjct: 421 DAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPN 480

[Close]

BLAST of Chy5G103970 vs. ExPASy TrEMBL
Match: A0A6J1DUW2 (tRNA (guanine(37)-N1)-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111024249 PE=3 SV=1)

HSP 1 Score: 833.6 bits (2152), Expect = 4.2e-238
Identity = 407/487 (83.57%), Postives = 439/487 (90.14%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHL LWALRIPRELCKAAIKILNGYLID+ R+KPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLKLWALRIPRELCKAAIKILNGYLIDRARVKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKVEELKGLCMYERVPYSLTLGYSYWGA--------------------- 120
           VQ PELS+IPE K+EELKGLC  E VPYSLTLGYSYWGA                     
Sbjct: 61  VQAPELSQIPEAKLEELKGLCEIELVPYSLTLGYSYWGADHILKQILPPRVEVPSSFETI 120

Query: 121 GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180
           GH+AHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVG+ITN+FRVPKFEIL GE+DMV
Sbjct: 121 GHIAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGTITNKFRVPKFEILMGEHDMV 180

Query: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240
           TEVKQYGATFKLDY LVYWNSRLEHEH+RLVSLFQPGE+ICDMFAGIGPFAIPAAQKECI
Sbjct: 181 TEVKQYGATFKLDYGLVYWNSRLEHEHVRLVSLFQPGEIICDMFAGIGPFAIPAAQKECI 240

Query: 241 VYANDLNPDSVRYLKVNAEINKVSGRIHVYNLDARKFISQLMIVPPNESNSESVTSILKA 300
           VY NDLNPDSV+YLKVNAEINKVS R+HVYNLDARKFISQLMIVP NESNSESVTS+LKA
Sbjct: 241 VYGNDLNPDSVQYLKVNAEINKVSDRVHVYNLDARKFISQLMIVPDNESNSESVTSVLKA 300

Query: 301 CEKGIGGTNQESPSANVQVQEIQDDIKVSNKSVEHSRSADISVAVLKRSSESCENEKDNG 360
           C+KGIGG+NQESPSANVQV+E+Q++ K+S+++VEHSRSADISV+VLKRSSESC  EKD+G
Sbjct: 301 CDKGIGGSNQESPSANVQVEEVQENSKISDETVEHSRSADISVSVLKRSSESC--EKDDG 360

Query: 361 IADDASTRLAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIKKK 420
           I DDAS  +AGR+  N NKRMKGS++ HARTWEHVDHVIMNLPASAL FLDAFRGSI++K
Sbjct: 361 IVDDASISVAGRKKRNANKRMKGSNICHARTWEHVDHVIMNLPASALQFLDAFRGSIRRK 420

Query: 421 YWKGSLPWIHCYCFMRANENQDFIISEAEFALNANIQDPIFHRVRDVAPNKAMYCLSFRL 467
           YWKGSLPWIHCYCFMRANENQDFIISEAE ALN  IQDP+FHRVRDVAPNKAMYCLSFRL
Sbjct: 421 YWKGSLPWIHCYCFMRANENQDFIISEAESALNGKIQDPVFHRVRDVAPNKAMYCLSFRL 480

[Close]

BLAST of Chy5G103970 vs. NCBI nr
Match: XP_011648665.1 (tRNA (guanine(37)-N1)-methyltransferase 2 isoform X2 [Cucumis sativus])

HSP 1 Score: 921 bits (2380), Expect = 0.0
Identity = 458/491 (93.28%), Postives = 466/491 (94.91%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKVEELKGLCMYERVPYSLTLGYSYWGA--------------------- 120
           VQTPELSEIPEMK+EELKGLCM+ERVPYSLTLGYSYWGA                     
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180
           GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV
Sbjct: 121 GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180

Query: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240
           TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI
Sbjct: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240

Query: 241 VYANDLNPDSVRYLKVNAEINKVSGRIHVYNLDARKFISQLMIVPPNESNSESVTSILKA 300
           VYANDLNPDS+RYLKVNAEINKVSGR+HVYNLDARKFISQLMIVPPNESNSESVTSILKA
Sbjct: 241 VYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKA 300

Query: 301 CEKGIGGTNQESPSANVQVQEIQDDIKVSNKSVEHSRSADISVAVLKRSSESCENEKDNG 360
           CEKGIGGTNQESP ANVQVQE+QD IK+SNKSVE SRSADISVAVLKRSSESCENEKDNG
Sbjct: 301 CEKGIGGTNQESPLANVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENEKDNG 360

Query: 361 IADDASTRLAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIKKK 420
           IADDASTR+AGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIKKK
Sbjct: 361 IADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIKKK 420

Query: 421 YWKGSLPWIHCYCFMRANENQDFIISEAEFALNANIQDPIFHRVRDVAPNKAMYCLSFRL 470
           YWKGSLPWIHCYCFMRANENQDFIISEAE ALNANIQDPIFHRVRDVAPNKAMYCLSFRL
Sbjct: 421 YWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSFRL 480

[Close]

BLAST of Chy5G103970 vs. NCBI nr
Match: XP_031736378.1 (tRNA (guanine(37)-N1)-methyltransferase 2 isoform X1 [Cucumis sativus])

HSP 1 Score: 915 bits (2365), Expect = 0.0
Identity = 458/495 (92.53%), Postives = 466/495 (94.14%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKVEELKGLCMYERVPYSLTLGYSYWGA--------------------- 120
           VQTPELSEIPEMK+EELKGLCM+ERVPYSLTLGYSYWGA                     
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180
           GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV
Sbjct: 121 GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180

Query: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240
           TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI
Sbjct: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240

Query: 241 VYANDLNPDSVRYLKVNAEINKVSGRIHVYNLDARKFISQLMIVPPNESNSESVTSILKA 300
           VYANDLNPDS+RYLKVNAEINKVSGR+HVYNLDARKFISQLMIVPPNESNSESVTSILKA
Sbjct: 241 VYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKA 300

Query: 301 CEKGIGGTNQESPSAN----VQVQEIQDDIKVSNKSVEHSRSADISVAVLKRSSESCENE 360
           CEKGIGGTNQESP AN    VQVQE+QD IK+SNKSVE SRSADISVAVLKRSSESCENE
Sbjct: 301 CEKGIGGTNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENE 360

Query: 361 KDNGIADDASTRLAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGS 420
           KDNGIADDASTR+AGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGS
Sbjct: 361 KDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGS 420

Query: 421 IKKKYWKGSLPWIHCYCFMRANENQDFIISEAEFALNANIQDPIFHRVRDVAPNKAMYCL 470
           IKKKYWKGSLPWIHCYCFMRANENQDFIISEAE ALNANIQDPIFHRVRDVAPNKAMYCL
Sbjct: 421 IKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCL 480

[Close]

BLAST of Chy5G103970 vs. NCBI nr
Match: TYK10701.1 (tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 910 bits (2351), Expect = 0.0
Identity = 450/471 (95.54%), Postives = 463/471 (98.30%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKVEELKGLCMYERVPYSLTLGYSYWGAGHVAHLNIHDELLPYKDVIAK 120
           VQTPELSEIPEMK+EELKGLCM+ERVPYSLTLGYSYWGAGHVAHLNIHDELLPYKDVIAK
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGAGHVAHLNIHDELLPYKDVIAK 120

Query: 121 VIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKLDYSLVYWNS 180
           VIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKLDYSLVYWNS
Sbjct: 121 VIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKLDYSLVYWNS 180

Query: 181 RLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLNPDSVRYLKVNAEIN 240
           RLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLNPDSVRYLKVNAEIN
Sbjct: 181 RLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLNPDSVRYLKVNAEIN 240

Query: 241 KVSGRIHVYNLDARKFISQLMIVPPNESNSESVTSILKACEKGIGGTNQESPSANVQVQE 300
           KVSGR+HVYNLDARKFISQLMIVPPNESNSE+VTSILKA E+GIGGTNQESP ANVQVQE
Sbjct: 241 KVSGRVHVYNLDARKFISQLMIVPPNESNSETVTSILKASEEGIGGTNQESPLANVQVQE 300

Query: 301 IQDDIKVSNKSVEHSRSADISVAVLKRSSESCENEKDNGIADDASTRLAGRRNGNGNKRM 360
           +QD I++SNK VEHSRSADISVAVLKRSSESCENEK++ IADDASTR+AGRRNGNGNKRM
Sbjct: 301 VQD-IEISNKFVEHSRSADISVAVLKRSSESCENEKEDRIADDASTRVAGRRNGNGNKRM 360

Query: 361 KGSS-VSHARTWEHVDHVIMNLPASALHFLDAFRGSIKKKYWKGSLPWIHCYCFMRANEN 420
           KGS+ VSHARTWEH+DHVIMNLPASALHFLDAFRGSIKKKYWKGSLPWIHCYCFMRANEN
Sbjct: 361 KGSTNVSHARTWEHIDHVIMNLPASALHFLDAFRGSIKKKYWKGSLPWIHCYCFMRANEN 420

Query: 421 QDFIISEAEFALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNEDTTS 470
           QDFIIS AE ALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNEDTTS
Sbjct: 421 QDFIISVAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNEDTTS 470

[Close]

BLAST of Chy5G103970 vs. NCBI nr
Match: XP_008458074.1 (PREDICTED: tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis melo])

HSP 1 Score: 897 bits (2319), Expect = 0.0
Identity = 450/492 (91.46%), Postives = 463/492 (94.11%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKVEELKGLCMYERVPYSLTLGYSYWGA--------------------- 120
           VQTPELSEIPEMK+EELKGLCM+ERVPYSLTLGYSYWGA                     
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180
           GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV
Sbjct: 121 GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180

Query: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240
           TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI
Sbjct: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240

Query: 241 VYANDLNPDSVRYLKVNAEINKVSGRIHVYNLDARKFISQLMIVPPNESNSESVTSILKA 300
           VYANDLNPDSVRYLKVNAEINKVSGR+HVYNLDARKFIS+LMIVPPNESNSE+VTSILKA
Sbjct: 241 VYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISRLMIVPPNESNSETVTSILKA 300

Query: 301 CEKGIGGTNQESPSANVQVQEIQDDIKVSNKSVEHSRSADISVAVLKRSSESCENEKDNG 360
            E+GIGGTNQESP ANVQVQE+QD I++SNK VEHSRSADISVAVLKRSSESCENEKD+ 
Sbjct: 301 SEEGIGGTNQESPLANVQVQEVQD-IEISNKFVEHSRSADISVAVLKRSSESCENEKDDR 360

Query: 361 IADDASTRLAGRRNGNGNKRMKGSS-VSHARTWEHVDHVIMNLPASALHFLDAFRGSIKK 420
           IADDASTR+AGRRNGNGNKRMKGS+ VSHARTWEH+DHVIMNLPASALHFLDAFRGSIKK
Sbjct: 361 IADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAFRGSIKK 420

Query: 421 KYWKGSLPWIHCYCFMRANENQDFIISEAEFALNANIQDPIFHRVRDVAPNKAMYCLSFR 470
           KYWKGSLPWIHCYCFMRANENQDFIIS AE ALNANIQDPIFHRVRDVAPNKAMYCLSFR
Sbjct: 421 KYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAMYCLSFR 480

[Close]

BLAST of Chy5G103970 vs. NCBI nr
Match: KAA0061573.1 (tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 895 bits (2314), Expect = 0.0
Identity = 451/501 (90.02%), Postives = 463/501 (92.42%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKVEELKGLCMYERVPYSLTLGYSYWGA--------------------- 120
           VQTPELSEIPEMK+EELKGLCM+ERVPYSLTLGYSYWGA                     
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 ---------GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFE 180
                    GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFE
Sbjct: 121 GDNFVLCFAGHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFE 180

Query: 181 ILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFA 240
           ILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFA
Sbjct: 181 ILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFA 240

Query: 241 IPAAQKECIVYANDLNPDSVRYLKVNAEINKVSGRIHVYNLDARKFISQLMIVPPNESNS 300
           IPAAQKECIVYANDLNPDSVRYLKVNAEINKVSGR+HVYNLDARKFISQLMIVPPNESNS
Sbjct: 241 IPAAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNS 300

Query: 301 ESVTSILKACEKGIGGTNQESPSANVQVQEIQDDIKVSNKSVEHSRSADISVAVLKRSSE 360
           E+VTSILKA E+GIGGTNQESP ANVQVQE+QD I++SNK VEHSRSADISVAVLKRSSE
Sbjct: 301 ETVTSILKASEEGIGGTNQESPLANVQVQEVQD-IEISNKFVEHSRSADISVAVLKRSSE 360

Query: 361 SCENEKDNGIADDASTRLAGRRNGNGNKRMKGSS-VSHARTWEHVDHVIMNLPASALHFL 420
           SCENEKD+ IADDASTR+AGRRNGNGNKRMKGS+ VSHARTWEH+DHVIMNLPASALHFL
Sbjct: 361 SCENEKDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFL 420

Query: 421 DAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAEFALNANIQDPIFHRVRDVAPN 470
           DAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIIS AE ALNANIQDPIFHRVRDVAPN
Sbjct: 421 DAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPN 480

[Close]

BLAST of Chy5G103970 vs. TAIR 10
Match: AT3G56120.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 580.5 bits (1495), Expect = 1.2e-165
Identity = 293/488 (60.04%), Postives = 356/488 (72.95%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           M DESKFDV+L LWALRIPRELCK+A +ILNGY+++ PRIKP+TEDPTC+K R VILSE 
Sbjct: 1   MFDESKFDVNLKLWALRIPRELCKSASRILNGYMLNMPRIKPITEDPTCEKTRLVILSES 60

Query: 61  VQTPELSEIPEMKVEELKGLCMYERVPYSLTLGYSYWGA--------------------- 120
           V+  +LSEIPE K+ +LK L   E VP+S+TLGYSYW A                     
Sbjct: 61  VKNADLSEIPEEKLNQLKKLSELEVVPHSVTLGYSYWSADHLLKQILPDGLDIPSSFETI 120

Query: 121 GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180
           GH+AHLN+HDELLP+KDVIAKVIYDKNYPRIKT+VNKVG+I+NEFRVPKFE+L GEN M 
Sbjct: 121 GHIAHLNLHDELLPFKDVIAKVIYDKNYPRIKTIVNKVGTISNEFRVPKFEVLAGENGME 180

Query: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240
           TEVKQYGA FKLDY LVYWNSRLEHEH+RL SLF+PGE +CDMFAGIGPFAIPAAQK C 
Sbjct: 181 TEVKQYGARFKLDYGLVYWNSRLEHEHMRLSSLFKPGETVCDMFAGIGPFAIPAAQKGCF 240

Query: 241 VYANDLNPDSVRYLKVNAEINKVSGRIHVYNLDARKFISQLMIVPPNESNSESVTSILKA 300
           VYANDLNPDSVRYLK+NA+ NKV   I V+N+DARKF S LM V   E N +SV    K 
Sbjct: 241 VYANDLNPDSVRYLKINAKFNKVDDLICVHNMDARKFFSHLMAVSTCEDNLQSVADNDKT 300

Query: 301 CEKGIGGTNQESPSANVQVQEIQDDIKVSNKSVEHSRSADISVAVLKRSSESCENEKDNG 360
            E  +    + + S         ++I+ SN S+     A+      K+ S + + E  NG
Sbjct: 301 KEAAVSRGGETNSSG--------EEIRESNASINEPLGAN------KKPSGTTKTE--NG 360

Query: 361 IADDASTRLAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIKKK 420
           +  D  +       G+ NKR++ + +  A+ WEH+DHVIMNLPASAL FLD+F   I+KK
Sbjct: 361 VGKDCKS-----IEGHANKRLRQTLLPIAKPWEHIDHVIMNLPASALQFLDSFSNVIQKK 420

Query: 421 YWKGSLPWIHCYCFMRANENQDFIISEAEFALNANIQDPIFHRVRDVAPNKAMYCLSFRL 468
           YWKG LP IHCYCF+RA+E  +FII+EAE AL  +I+DP+FH+VRDVAPNKAM+CLSFRL
Sbjct: 421 YWKGPLPLIHCYCFIRASETTEFIIAEAETALKFHIEDPVFHKVRDVAPNKAMFCLSFRL 467

[Close]

BLAST of Chy5G103970 vs. TAIR 10
Match: AT4G27340.1 (Met-10+ like family protein )

HSP 1 Score: 192.6 bits (488), Expect = 7.2e-49
Identity = 132/438 (30.14%), Postives = 187/438 (42.69%), Query Frame = 0


Query: 53  RYVILSEKVQTPELSEIPE------MKVEELKGLCMYERVPYSLTLGYSYWG-------- 112
           R ++L EK    E+ ++PE       + +       +E V   LTL Y YW         
Sbjct: 274 RLLLLDEKYSGEEVQDLPEAIKVLFAEAKMADASLSFELVKCRLTLFYDYWPMIEILEAV 333

Query: 113 -------------AGHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFR 172
                         GH+AHLN+ DE LPYK +IAKV+ DKN P+I+TVVNK+  I N+FR
Sbjct: 334 LPKGMIVPSAFEMVGHIAHLNLRDEHLPYKRLIAKVVLDKNQPKIQTVVNKIDPIHNDFR 393

Query: 173 VPKFEILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAG 232
             + E+L G + +VT V + G  F +D + VYWNS+L  E  RL+  F   +V+CD+FAG
Sbjct: 394 TMQLEVLAGNHSLVTLVVENGLRFHVDLARVYWNSKLGTERQRLLLGFDQNDVVCDVFAG 453

Query: 233 IGPFAIPAAQKECIVYANDLNPDSVRYLKVNAEINKVSGRIHVYNLDARKFISQLMIVPP 292
           +GP A+ AA+    VYANDLNP +V +++ N+ +NK+  RI ++N+D R+FI  +     
Sbjct: 454 VGPIALAAARIVKRVYANDLNPHAVEFMEQNSVVNKLEKRIEIFNMDGRRFIKAMF---- 513

Query: 293 NESNSESVTSILKACEKGIGGTNQESPSANVQVQEIQDDIKVSNKSVEHSRSADISVAVL 352
                        + EKG                                          
Sbjct: 514 -------------SSEKG------------------------------------------ 573

Query: 353 KRSSESCENEKDNGIADDASTRLAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASA 412
                                                         + V  V+MNLP  A
Sbjct: 574 ----------------------------------------------QKVTQVVMNLPKDA 606

Query: 413 LHFLDAFRGSIKKKYWKG--SLPWIHCYCFMRANENQDFIISEAEFALNANIQDPIFHRV 462
              LDAFRG    ++     S P IH Y F +A++ +         AL+    D    +V
Sbjct: 634 AESLDAFRGVYNDRHRDEGLSFPTIHVYGFSKASDPEFDFHERIRIALSEVAVDVKMRKV 606

[Close]

BLAST of Chy5G103970 vs. TAIR 10
Match: AT4G04670.1 (Met-10+ like family protein / kelch repeat-containing protein )

HSP 1 Score: 56.6 bits (135), Expect = 6.2e-08
Identity = 37/115 (32.17%), Postives = 57/115 (49.57%), Query Frame = 0


Query: 142 NEFRVPKFEILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICD 201
           N  R    EIL G+N  V   ++ G  +  D +   ++     E +R+ ++    EV+ D
Sbjct: 785 NGTRDSTLEILVGDNGWVNH-RENGILYSFDATKCMFSWGNLSEKLRMGNMACENEVVVD 844

Query: 202 MFAGIGPFAIP--AAQKECIVYANDLNPDSVRYLKVNAEINKVSGRIHVYNLDAR 255
           +FAGIG F +P     K  +VYA + NP ++  L+ N E N VS R  +   D R
Sbjct: 845 LFAGIGYFVLPFLVRAKAKLVYACEWNPHAIEALRRNVEANSVSERCIILEGDNR 898

The following BLAST results are available for this feature:

BLAST of Chy5G103970 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F6HH45	1.3e-180	64.87	tRNA (guanine(37)-N1)-methyltransferase 2 OS=Vitis vinifera OX=29760 GN=VIT_11s0...	[more]
Q93YU6	1.7e-164	60.04	tRNA (guanine(37)-N1)-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=At3...	[more]
C5XX79	2.3e-161	61.46	tRNA (guanine(37)-N1)-methyltransferase OS=Sorghum bicolor OX=4558 GN=Sb04g02539...	[more]
A9T6G5	4.5e-104	44.49	tRNA (guanine(37)-N1)-methyltransferase OS=Physcomitrium patens OX=3218 GN=PHYPA...	[more]
F4NUJ6	8.3e-66	32.92	tRNA (guanine(37)-N1)-methyltransferase OS=Batrachochytrium dendrobatidis (strai...	[more]

BLAST of Chy5G103970 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LFW8	1.5e-264	92.70	tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G0053...	[more]
A0A5D3CFY1	4.2e-262	95.54	tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A1S3C7L3	2.1e-258	91.46	tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103497610 ...	[more]
A0A5A7V0B1	8.1e-258	90.02	tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A6J1DUW2	4.2e-238	83.57	tRNA (guanine(37)-N1)-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111...	[more]

BLAST of Chy5G103970 vs. NCBI nr


Match Name	Identity	Description
XP_011648665.1	93.28	tRNA (guanine(37)-N1)-methyltransferase 2 isoform X2 [Cucumis sativus]	[more]
XP_031736378.1	92.53	tRNA (guanine(37)-N1)-methyltransferase 2 isoform X1 [Cucumis sativus]	[more]
TYK10701.1	95.54	tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis melo var. makuwa]	[more]
XP_008458074.1	91.46	PREDICTED: tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis melo]	[more]
KAA0061573.1	90.02	tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis melo var. makuwa]	[more]

BLAST of Chy5G103970 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G56120.1	1.2e-165	60.04	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT4G27340.1	7.2e-49	30.14	Met-10+ like family protein	[more]
AT4G04670.1	6.2e-08	32.17	Met-10+ like family protein / kelch repeat-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR030382	SAM-dependent methyltransferase TRM5/TYW2-type	PFAM	PF02475	Met_10	coord: 96..411 e-value: 1.8E-64 score: 217.2
IPR030382	SAM-dependent methyltransferase TRM5/TYW2-type	PROSITE	PS51684	SAM_MT_TRM5_TYW2	coord: 96..461 score: 102.409431
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 159..459 e-value: 2.1E-57 score: 195.7
None	No IPR available	GENE3D	3.30.300.110		coord: 85..156 e-value: 1.3E-18 score: 68.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 334..364
None	No IPR available	PANTHER	PTHR23245	TRNA METHYLTRANSFERASE	coord: 2..465
None	No IPR available	PANTHER	PTHR23245:SF36	TRNA (GUANINE(37)-N1)-METHYLTRANSFERASE	coord: 2..465
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 199..256 e-value: 4.06067E-4 score: 37.7947
IPR025792	tRNA (guanine(37)-N(1))-methyltransferase, eukaryotic	HAMAP	MF_03152	TRM5	coord: 2..461 score: 23.444159
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 97..461

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy5G103970.1	Chy5G103970.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030488	tRNA methylation
cellular_component	GO:0005759	mitochondrial matrix
cellular_component	GO:0005634	nucleus
molecular_function	GO:0052906	tRNA (guanine(37)-N(1))-methyltransferase activity
molecular_function	GO:0009019	tRNA (guanine-N1-)-methyltransferase activity