Chy5G103960 (gene) Cucumber (hystrix) v1

Overview
NameChy5G103960
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Description(1->3)-beta-glucan endohydrolase
LocationchrH05: 17630470 .. 17632753 (-)
RNA-Seq ExpressionChy5G103960
SyntenyChy5G103960
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTACTCATCAGCTCCCACCAGAGAAAGTTGTTAAAATGCTTCAAGAAAATGGGTTTCATAAATTGAAGCTTTTTGAAGCTGATGATAGGATTTTGGAAGCACTTATTGGAAGTGATATTGAGGTTATGTTGGCTATTCCTAATTCAATGTTGTTTCTTATGAGTCAAGATCCTCACGCTGCTGCTTCTTGGGTTGATTCTAATGTTACTGCTTATACTTATCATGGTGGTGTCAAAATCAAGTTGAGTTTTGAAGATTCTTTTGATTAATTTTGATCTTCTTTGATTTGTTTTTTTCTCGTTTTTTTGGTGCTCTTTAGGTGTTTGATGAATTTCCCACTTCAGGGTTTCTCTGTTTTTAATATGTTGTTCTTTTTTGTTCATTCAGCTGCTGCTATCCTGCTGATGATGCTTTCATTTTCTTTGTTTACTTATTCGAAAAGCCGTTAACGTAGCTTAGAGGATATTTGCAAGAAAGGGTCATAATCACTTTTTTTTCATCACTTTTGTCATGTTTAAAGTCACAGCTTATATATGTCGATTTAGAATCAATTTATACCATCCAAATTGATTTTGAATGATTTAATGCATGGTTCGTTGTGATTTTGAACATGATAAAAAGTTATTTTAATCATTTCAAAATTACTTCAAAAAATGTTCTAAACATTATTTTTAGTTCGCTAAAATGAAATTTTCAGTTTTCTAAAAACCACGTCGGTTAATCTTAGTGTTACTAATTTCAAGTTAGTTTGATTGATCATTTGGTCATGTTCTTTAATGGATTGTAGCTACTAGTTTAAATGATTAAATATAAGCTAAACATGATATTGAAAGGATTAAAATCAGCTAAATAACTTTTCACTCTTCAAAAAGTCATGTTGGGTAATCACAGTGTCACTATGTCACTAAACATGATATTATCAGCTGGGTTGAACTTAGTTTCATTGATCACTTCACAAGGAAGTACTATTTGAGTTATAAAACTATTGAAAAGTAATCAAATGCCCCTCTTAAATAATGGTCATTTCTTGAAGAACCATTCTTTTGGCTGAAAAAGTAGAGATTTATGAAGTTAAAATTAGCTAAAAAATCATATCAGTTCTTTATCTTTTCCATAAAATTCCATTGAAACTGATTTTGCAGGTATGTTGCTGTAGGCAATGAGCCCTTTCTCAAGTCTTACAATGGCACTTACCTTCAGTTAACATTGCCTGCTCTAAGGAATATCCAACAATCTCTAAATGATGCAGGGCTTGGTTCAAAGGTCAAAGCTACTATTCCTTTCAATGCTGATATTTACAACTCCCCCGACTCAAACCCGGTTCCATCTGCAGGCGAGTTCCGTCCAGATGTCCGAGACTTAACGATCGAGATTCTACACTACCTATCCATAAACAACGCACCATTCACAGTCAATATCTACCCCTTCCTTTCCCTATATGGAAATGACTACTTTCCAATAGACTTTGCATTTTTTGATGGAACAGCATATAGGCCAATCAAAGACAGAGATCTAACGTACACGAATGTGTTTGATGCAAATTTCGACACACTCGTATCAGCTCTAGCAAAAGCAGGATACCCTGATATGAAGATCATCGTAGGAGAAGTTGGTTGGCCAACGGACGGCGACAAACACGCCAATATACAAAATGCGAAACGATTCAACCAAGGATTGTTAAGACATGCTTTAAGTGGACAGGGAACTCCAGCAAGGGTAGGCATAATTGATGTCTATCTTTTTAGTCTAATTGATGAAAATGCTAAAAGTATAGAACCAGGGAGCTTTGAAAGGCATTGGGGAATATTCGAATTTGACGGTAAACCAAAATATGAACTAGACTTGGCTGGAACAGAAGAAGAAAAAGGGTTAATACCAGTTGAAGGAGTGAGATATATGGGGAAAAGATGGTGTGTTTTGAATCCAAATGTGAATGATTGGGAAGATTTGGCTGATAGTGTAGACTATGCTTGTAGTTTATCAGATTGTACTGCATTGGAATATGGATCTTCTTGCAACCAATTAACTGCACAAGAAAATGCTTCTTATGCATTTAATATGTACTATCAAGTGAACAGCCAAAAGAGTTGGAATTGTGATTTTGATGGTTTGGCTGTGGTGACTCAACAAGATCCTTCTTATGGAAACTGCCAGTTCCCAATTATGATTGATTATACCTCTTCTTCTTCTTCATTTCTGGTTGATGAAAGATTTTCTGATCTTCTTGTTGCAGTTTTTTTGGGAATTGTAGTGTTTTTTATTGTGCAATATGAACAGTGA

mRNA sequence

ATGGCTACTCATCAGCTCCCACCAGAGAAAGTTGTTAAAATGCTTCAAGAAAATGGGTTTCATAAATTGAAGCTTTTTGAAGCTGATGATAGGATTTTGGAAGCACTTATTGGAAGTGATATTGAGGTTATGTTGGCTATTCCTAATTCAATGTTGTTTCTTATGAGTCAAGATCCTCACGCTGCTGCTTCTTGGGTTGATTCTAATGTTACTGCTTATACTTATCATGGTGGTGTCAAAATCAAGTATGTTGCTGTAGGCAATGAGCCCTTTCTCAAGTCTTACAATGGCACTTACCTTCAGTTAACATTGCCTGCTCTAAGGAATATCCAACAATCTCTAAATGATGCAGGGCTTGGTTCAAAGGTCAAAGCTACTATTCCTTTCAATGCTGATATTTACAACTCCCCCGACTCAAACCCGGTTCCATCTGCAGGCGAGTTCCGTCCAGATGTCCGAGACTTAACGATCGAGATTCTACACTACCTATCCATAAACAACGCACCATTCACAGTCAATATCTACCCCTTCCTTTCCCTATATGGAAATGACTACTTTCCAATAGACTTTGCATTTTTTGATGGAACAGCATATAGGCCAATCAAAGACAGAGATCTAACGTACACGAATGTGTTTGATGCAAATTTCGACACACTCGTATCAGCTCTAGCAAAAGCAGGATACCCTGATATGAAGATCATCGTAGGAGAAGTTGGTTGGCCAACGGACGGCGACAAACACGCCAATATACAAAATGCGAAACGATTCAACCAAGGATTGTTAAGACATGCTTTAAGTGGACAGGGAACTCCAGCAAGGGTAGGCATAATTGATGTCTATCTTTTTAGTCTAATTGATGAAAATGCTAAAAGTATAGAACCAGGGAGCTTTGAAAGGCATTGGGGAATATTCGAATTTGACGGTAAACCAAAATATGAACTAGACTTGGCTGGAACAGAAGAAGAAAAAGGGTTAATACCAGTTGAAGGAGTGAGATATATGGGGAAAAGATGGTGTGTTTTGAATCCAAATGTGAATGATTGGGAAGATTTGGCTGATAGTGTAGACTATGCTTGTAGTTTATCAGATTGTACTGCATTGGAATATGGATCTTCTTGCAACCAATTAACTGCACAAGAAAATGCTTCTTATGCATTTAATATGTACTATCAAGTGAACAGCCAAAAGAGTTGGAATTGTGATTTTGATGGTTTGGCTGTGGTGACTCAACAAGATCCTTCTTATGGAAACTGCCAGTTCCCAATTATGATTGATTATACCTCTTCTTCTTCTTCATTTCTGGTTGATGAAAGATTTTCTGATCTTCTTGTTGCAGTTTTTTTGGGAATTGTAGTGTTTTTTATTGTGCAATATGAACAGTGA

Coding sequence (CDS)

ATGGCTACTCATCAGCTCCCACCAGAGAAAGTTGTTAAAATGCTTCAAGAAAATGGGTTTCATAAATTGAAGCTTTTTGAAGCTGATGATAGGATTTTGGAAGCACTTATTGGAAGTGATATTGAGGTTATGTTGGCTATTCCTAATTCAATGTTGTTTCTTATGAGTCAAGATCCTCACGCTGCTGCTTCTTGGGTTGATTCTAATGTTACTGCTTATACTTATCATGGTGGTGTCAAAATCAAGTATGTTGCTGTAGGCAATGAGCCCTTTCTCAAGTCTTACAATGGCACTTACCTTCAGTTAACATTGCCTGCTCTAAGGAATATCCAACAATCTCTAAATGATGCAGGGCTTGGTTCAAAGGTCAAAGCTACTATTCCTTTCAATGCTGATATTTACAACTCCCCCGACTCAAACCCGGTTCCATCTGCAGGCGAGTTCCGTCCAGATGTCCGAGACTTAACGATCGAGATTCTACACTACCTATCCATAAACAACGCACCATTCACAGTCAATATCTACCCCTTCCTTTCCCTATATGGAAATGACTACTTTCCAATAGACTTTGCATTTTTTGATGGAACAGCATATAGGCCAATCAAAGACAGAGATCTAACGTACACGAATGTGTTTGATGCAAATTTCGACACACTCGTATCAGCTCTAGCAAAAGCAGGATACCCTGATATGAAGATCATCGTAGGAGAAGTTGGTTGGCCAACGGACGGCGACAAACACGCCAATATACAAAATGCGAAACGATTCAACCAAGGATTGTTAAGACATGCTTTAAGTGGACAGGGAACTCCAGCAAGGGTAGGCATAATTGATGTCTATCTTTTTAGTCTAATTGATGAAAATGCTAAAAGTATAGAACCAGGGAGCTTTGAAAGGCATTGGGGAATATTCGAATTTGACGGTAAACCAAAATATGAACTAGACTTGGCTGGAACAGAAGAAGAAAAAGGGTTAATACCAGTTGAAGGAGTGAGATATATGGGGAAAAGATGGTGTGTTTTGAATCCAAATGTGAATGATTGGGAAGATTTGGCTGATAGTGTAGACTATGCTTGTAGTTTATCAGATTGTACTGCATTGGAATATGGATCTTCTTGCAACCAATTAACTGCACAAGAAAATGCTTCTTATGCATTTAATATGTACTATCAAGTGAACAGCCAAAAGAGTTGGAATTGTGATTTTGATGGTTTGGCTGTGGTGACTCAACAAGATCCTTCTTATGGAAACTGCCAGTTCCCAATTATGATTGATTATACCTCTTCTTCTTCTTCATTTCTGGTTGATGAAAGATTTTCTGATCTTCTTGTTGCAGTTTTTTTGGGAATTGTAGTGTTTTTTATTGTGCAATATGAACAGTGA

Protein sequence

MATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIGSDIEVMLAIPNSMLFLMSQDPHAAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQSLNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDLTIEILHYLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTAYRPIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGQGTPARVGIIDVYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVNDWEDLADSVDYACSLSDCTALEYGSSCNQLTAQENASYAFNMYYQVNSQKSWNCDFDGLAVVTQQDPSYGNCQFPIMIDYTSSSSSFLVDERFSDLLVAVFLGIVVFFIVQYEQ*
Homology
BLAST of Chy5G103960 vs. ExPASy Swiss-Prot
Match: Q6NKW9 (Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana OX=3702 GN=At1g64760 PE=2 SV=2)

HSP 1 Score: 526.2 bits (1354), Expect = 3.8e-148
Identity = 259/448 (57.81%), Postives = 322/448 (71.88%), Query Frame = 0

Query: 1   MATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIGSDIEVMLAIPNSMLFLMSQDPH 60
           MATH+LPP+ VV+ML++N  +K+KLF+AD+  + AL GS +EVM+AIPN  L +M+    
Sbjct: 32  MATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGSGLEVMVAIPNDQLKVMTSYDR 91

Query: 61  AAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQSLNDAGLG 120
            A  WV  NVT Y + GGV I +VAVGNEPFLKSYNG+++ LT PAL NIQ +LN+AGLG
Sbjct: 92  -AKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFINLTFPALANIQNALNEAGLG 151

Query: 121 SKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDLTIEILHYLSINNAPFTVNIYPFLSL 180
           + VKAT+P NAD+Y+SP SNPVPSAG FRPD+     +I+ +L  NNAP T+NIYPFLSL
Sbjct: 152 NSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIVDFLGKNNAPITINIYPFLSL 211

Query: 181 YGNDYFPIDFAFFDGTAYRPIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVGW 240
           YGND FP+++AFFDG    PI D  + YTNVFDANFDTLVS+L   G+ DM IIVGEVGW
Sbjct: 212 YGNDDFPLNYAFFDGA--EPINDNGIDYTNVFDANFDTLVSSLKAVGHGDMPIIVGEVGW 271

Query: 241 PTDGDKHANIQNAKRFNQGLLRHALSGQGTPARVGIIDVYLFSLIDENAKSIEPGSFERH 300
           PT+GDKHAN  +A RF  GLL    + +GTP R   I+VYLF L+DE+AKSI PG FERH
Sbjct: 272 PTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPTYIEVYLFGLLDEDAKSIAPGPFERH 331

Query: 301 WGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVNDWEDLADSVDYACS 360
           WGIF+FDG+PK+ +DL+G  + K LI  + V Y+  +WC  NP   D   LA ++DYAC+
Sbjct: 332 WGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWCTFNPEAKDLTKLAANIDYACT 391

Query: 361 LSDCTALEYGSSCNQLTAQENASYAFNMYYQVNSQKSWNCDFDGLAVVTQQDPSYGNCQF 420
            SDCTAL YGSSCN L A  NASYAFNM++QV +Q    C F GLA +T Q+ S G C F
Sbjct: 392 FSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESACYFQGLATITTQNISQGQCNF 451

Query: 421 PIMIDYTSSSSSFLVDERFSDLLVAVFL 449
           PI I   SS+SSF        +L+  FL
Sbjct: 452 PIQI-VASSASSFSCSSYSLVVLIVWFL 475

BLAST of Chy5G103960 vs. ExPASy Swiss-Prot
Match: Q9M088 (Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana OX=3702 GN=At4g31140 PE=2 SV=1)

HSP 1 Score: 480.7 bits (1236), Expect = 1.8e-134
Identity = 236/456 (51.75%), Postives = 317/456 (69.52%), Query Frame = 0

Query: 2   ATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIGSDIEVMLAIPNSMLFLMSQDPHA 61
           A H LPP  VV++L+ENG  K+KLFEAD  IL+AL  + I+VM+ IPN +L  ++    A
Sbjct: 35  ARHPLPPATVVRLLRENGIQKVKLFEADSAILKALSRTGIQVMVGIPNDLLAPLAGSVAA 94

Query: 62  AASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQSLNDAGLGS 121
           A  WV  NV+A+    GV I+YVAVGNEPFLK++NGT+  +TLPAL+NIQ ++  AGL +
Sbjct: 95  AERWVSQNVSAHVSSNGVDIRYVAVGNEPFLKAFNGTFEGITLPALQNIQSAIIKAGLAT 154

Query: 122 KVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDLTIEILHYLSINNAPFTVNIYPFLSLY 181
           +VK T+P NAD+Y S  ++ +PS G+FRP++RDL + I+ +LS N APFT+NIYPF+SLY
Sbjct: 155 QVKVTVPLNADVYQS--ASNLPSDGDFRPEIRDLMLNIVKFLSDNGAPFTINIYPFISLY 214

Query: 182 GNDYFPIDFAFFDGTAYRPIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVGWP 241
            +  FP++FAFFDGT   PI D    Y NV DAN+DTLV +L K G+ ++ IIVGEVGWP
Sbjct: 215 NDPNFPVEFAFFDGTG-TPINDNGRIYDNVLDANYDTLVWSLQKNGFGNLTIIVGEVGWP 274

Query: 242 TDGDKHANIQNAKRFNQGLLRHALSGQGTPARVGIIDVYLFSLIDENAKSIEPGSFERHW 301
           TDGDK+AN+  A+R+NQG +    + +GTP R G +D YLF LIDE+AKSI+PG+FERHW
Sbjct: 275 TDGDKNANLMYARRYNQGFMNRQKANKGTPMRPGAMDAYLFGLIDEDAKSIQPGNFERHW 334

Query: 302 GIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVNDWE-DLADSVDYACS 361
           GIF  DG+PKY+L L       GLIP + V Y+ K+WC+L PN N  +  L  SV YAC 
Sbjct: 335 GIFYIDGQPKYQLSLG---SGNGLIPAKDVHYLAKKWCILAPNANLQDPQLGPSVSYACD 394

Query: 362 LSDCTALEYGSSCNQLTAQENASYAFNMYYQVNSQKSWNCDFDGLAVVTQQDPSYGNCQF 421
            +DCT+L YGSSC  L   +N SYAFN YYQV++Q    C F GL++V+ +DPS G+C+F
Sbjct: 395 HADCTSLGYGSSCGNLNLAQNVSYAFNSYYQVSNQLDSACKFPGLSIVSTRDPSVGSCKF 454

Query: 422 PIMIDYTSSSSSFLVDERFSDLLVAVFLGIVVFFIV 457
            IMI    +S +  +        V + L I ++ ++
Sbjct: 455 KIMIKSEDASEASAMMPITRSTAVLLLLSICLYIVL 484

BLAST of Chy5G103960 vs. ExPASy Swiss-Prot
Match: Q93Z08 (Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana OX=3702 GN=At5g58090 PE=2 SV=2)

HSP 1 Score: 479.2 bits (1232), Expect = 5.3e-134
Identity = 236/425 (55.53%), Postives = 300/425 (70.59%), Query Frame = 0

Query: 2   ATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIGSDIEVMLAIPNSMLFLMSQDPHA 61
           A+H LPP+ VV+ML+ENG  K+KLF+A+   L AL  S IEVM+ IPN ML  ++    A
Sbjct: 30  ASHPLPPDIVVRMLRENGIQKVKLFDAEYDTLRALGKSGIEVMVGIPNEMLATLASSLKA 89

Query: 62  AASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQSLNDAGLGS 121
           A  WV  NV+ +     V I+YVAVGNEPFL +YNG+YL  T PALRNIQ ++  AGL +
Sbjct: 90  AEKWVAKNVSTHISTDNVNIRYVAVGNEPFLSTYNGSYLSTTFPALRNIQIAIIKAGLQN 149

Query: 122 KVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDLTIEILHYLSINNAPFTVNIYPFLSLY 181
           +VK T P NAD+Y+S  S   PS G+FR ++RDL I I+ +LS N  PFTVNIYP++SLY
Sbjct: 150 QVKVTCPLNADVYDS--STTFPSGGDFRANIRDLMITIVKFLSENGGPFTVNIYPYISLY 209

Query: 182 GNDYFPIDFAFFDGTAYRPIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVGWP 241
            N  FP+D+AFFDG A +P+ D    Y N+FDAN+DTLV AL K G+ +M II+GE+GWP
Sbjct: 210 TNPDFPVDYAFFDGNA-QPLNDGGTFYYNMFDANYDTLVHALEKNGFGNMPIIIGEIGWP 269

Query: 242 TDGDKHANIQNAKRFNQGLLRHALSGQGTPARVGIIDVYLFSLIDENAKSIEPGSFERHW 301
           TDGD +AN+  AK+FNQG + H   G+GTP R G ID YLFSLIDE+AKS++PG FERHW
Sbjct: 270 TDGDSNANLDYAKKFNQGFMAHISGGKGTPRRPGPIDAYLFSLIDEDAKSVQPGYFERHW 329

Query: 302 GIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVN-DWEDLADSVDYACS 361
           GIF FDG PKY L+L GT     LI  +GVRY+ ++WCV+ PNV  D   +A +V YACS
Sbjct: 330 GIFTFDGLPKYALNL-GTTNTGALIQAKGVRYLERKWCVMKPNVRLDDPQVAPAVSYACS 389

Query: 362 LSDCTALEYGSSCNQLTAQENASYAFNMYYQVNSQKSWNCDFDGLAVVTQQDPSYGNCQF 421
           L DCT+L  G+SC  L  ++N SYAFN YYQ+  Q    C F  ++ VT+ DPS G C+F
Sbjct: 390 LGDCTSLGVGTSCANLDGKQNISYAFNSYYQIQDQLDTACKFPNISEVTKTDPSTGTCRF 449

Query: 422 PIMID 426
           PIMI+
Sbjct: 450 PIMIE 450

BLAST of Chy5G103960 vs. ExPASy Swiss-Prot
Match: Q9FGH4 (Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana OX=3702 GN=At5g58480 PE=2 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 7.3e-91
Identity = 179/432 (41.44%), Postives = 263/432 (60.88%), Query Frame = 0

Query: 2   ATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIGSDIEVMLAIPNSMLFLMSQDPHA 61
           A+H LPP KVV++L+ NG  K+KLF+AD ++L AL GS+I V + I NSML  ++     
Sbjct: 34  ASHPLPPSKVVELLKSNGIVKVKLFDADPKVLRALSGSNIGVTIGIQNSMLKSLNASVKV 93

Query: 62  AASWVDSNVTAYTYHGG--VKIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQSLNDAGL 121
           A SWV  NVT Y ++GG  V+I+YVAVG EPFL+SY   Y    + A  NIQ +L  A L
Sbjct: 94  AESWVHDNVTRY-FNGGNRVRIEYVAVGEEPFLQSYGNQYKPFVIGAAMNIQNALVKANL 153

Query: 122 GSKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDLTIEILHYLSINNAPFTVNIYPFLS 181
            ++VK  +P + D + S      PS+G FR D+    IE+L +L+ +++PF V I PFLS
Sbjct: 154 ANEVKVVVPSSFDSFLSESGR--PSSGHFRADLNKTMIELLSFLTKHHSPFFVTISPFLS 213

Query: 182 LYGNDYFPIDFAFFDGTAYRPIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVG 241
            + N    +DF+ F  TA +  KD   TY N FD ++DTLVSAL   G+ ++ I+V ++G
Sbjct: 214 FHQNKNISLDFSLFKETA-KAHKDGRKTYRNSFDLSYDTLVSALFTIGFSEVDIVVSKIG 273

Query: 242 WPTDGDKHANIQNAKRFNQGLLRHALSGQGTPARVGIIDVYLFSLIDENAKSIEPGSFER 301
           WPTDG ++A    A+ F +GL+ H      +  R   ++ Y+ SL+DE+ +++  G+FER
Sbjct: 274 WPTDGAENATSLTAEAFFKGLIVHLEKKTASLPRPP-VETYIESLLDEDQRNLSAGNFER 333

Query: 302 HWGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVNDWEDLADSVDYAC 361
           HWG+F FDG+ KY          K  +  + V+Y+  +WCV+N N  D  + +     AC
Sbjct: 334 HWGVFTFDGQAKYNFSF----NHKNQVNAQNVQYLPPKWCVVNNN-KDLSNASARALEAC 393

Query: 362 SLSDCTALEYGSSCNQLTAQENASYAFNMYYQVNSQKSWNCDFDGLAVVTQQDPSYGNCQ 421
           +++DCT++  G SC+ +    N SYAFN  YQ N   + +C+F GL ++T  DPS  NC+
Sbjct: 394 AVADCTSILPGGSCSGIRWPGNVSYAFNSLYQQNDHSAESCNFGGLGLITTVDPSEDNCR 453

Query: 422 FPIMIDYTSSSS 432
           F I +D + SSS
Sbjct: 454 FSIQLDTSHSSS 455

BLAST of Chy5G103960 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 238.8 bits (608), Expect = 1.2e-61
Identity = 147/424 (34.67%), Postives = 227/424 (53.54%), Query Frame = 0

Query: 7   PPEKVVKMLQENGFHKLKLFEADDRILEALIGSDIEVMLAIPNSMLFLMSQDPHAAASWV 66
           PP + VK+LQ     K++L+ AD  I++AL G+ + +++   N  +  ++ DP+AA  W+
Sbjct: 39  PPSETVKLLQSTSIQKVRLYGADPAIIKALAGTGVGIVIGAANGDVPSLASDPNAATQWI 98

Query: 67  DSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQSLNDAGLGSKVKAT 126
           +SNV    ++   KI  + VGNE  L S +   +   LPA++N+Q++L    LG K+K +
Sbjct: 99  NSNV--LPFYPASKIMLITVGNE-ILMSNDPNLVNQLLPAMQNVQKALEAVSLGGKIKVS 158

Query: 127 IPFNADIYNSPDSNPVPSAGEFRPDVRDLTIEILHYLSINNAPFTVNIYPFLSLYGNDYF 186
              +  +  S D    PS+G F    +     IL +LS   +PF +N YPF + Y +D  
Sbjct: 159 TVNSMTVLGSSDP---PSSGSFAAGYQTGLKGILQFLSDTGSPFAINPYPFFA-YQSDPR 218

Query: 187 P--IDFAFFDGTAYRPIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVGWPTDG 246
           P  + F  F+  A R      + YTN+FDA  D + SAL   G+  ++I+V E GW + G
Sbjct: 219 PETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVHSALKSMGFEKVEIVVAETGWASRG 278

Query: 247 DKH---ANIQNAKRFNQGLLRHALSGQGTPARVG-IIDVYLFSLIDENAKSIEPG-SFER 306
           D +   A++ NAK +N  L+ H  S  GTP   G  +D Y+F+L DEN K   PG S ER
Sbjct: 279 DANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKPVDTYIFALYDENLK---PGPSSER 338

Query: 307 HWGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVNDWEDLADSVDYAC 366
            +G+F+ D    Y++ LA +            +     WCV      + E+L  S+D+AC
Sbjct: 339 AFGLFKTDLSMVYDVGLAKSSSSS---QTPSGKVTSSGWCVPKKGATN-EELQASLDWAC 398

Query: 367 SLS-DCTALEYGSSC-NQLTAQENASYAFNMYYQVNSQKSWNCDFDGLAVVTQQDPSYGN 422
               DC A++ G +C        +A+YA NMY+Q + ++  +CDF   A VT Q+PSY N
Sbjct: 399 GHGIDCGAIQPGGACFEPNNVVSHAAYAMNMYFQKSPKQPTDCDFSKTATVTSQNPSYNN 448

BLAST of Chy5G103960 vs. ExPASy TrEMBL
Match: A0A0A0LHZ1 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_2G005360 PE=3 SV=1)

HSP 1 Score: 924.1 bits (2387), Expect = 2.3e-265
Identity = 453/463 (97.84%), Postives = 456/463 (98.49%), Query Frame = 0

Query: 1   MATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIGSDIEVMLAIPNSMLFLMSQDPH 60
           MATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIGSDIEVMLAIPNSMLFLMSQDPH
Sbjct: 16  MATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIGSDIEVMLAIPNSMLFLMSQDPH 75

Query: 61  AAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQSLNDAGLG 120
           AAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYL LTLPALRNIQQSLNDAGLG
Sbjct: 76  AAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLPLTLPALRNIQQSLNDAGLG 135

Query: 121 SKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDLTIEILHYLSINNAPFTVNIYPFLSL 180
           SKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDLTIEILHYLSINNAPFTVNIYPFLSL
Sbjct: 136 SKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDLTIEILHYLSINNAPFTVNIYPFLSL 195

Query: 181 YGNDYFPIDFAFFDGTAYRPIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVGW 240
           YGNDYFPIDFAFFDGTAYRPIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVGW
Sbjct: 196 YGNDYFPIDFAFFDGTAYRPIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVGW 255

Query: 241 PTDGDKHANIQNAKRFNQGLLRHALSGQGTPARVGIIDVYLFSLIDENAKSIEPGSFERH 300
           PTDGDKHAN+QNAKRFNQGLLRHALSGQGTPARVGIIDVYLFSLIDENAKSIEPGSFERH
Sbjct: 256 PTDGDKHANVQNAKRFNQGLLRHALSGQGTPARVGIIDVYLFSLIDENAKSIEPGSFERH 315

Query: 301 WGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVNDWEDLADSVDYACS 360
           WGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVNDWE LADSVDYACS
Sbjct: 316 WGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVNDWEGLADSVDYACS 375

Query: 361 LSDCTALEYGSSCNQLTAQENASYAFNMYYQVNSQKSWNCDFDGLAVVTQQDPSYGNCQF 420
           LSDCTALEYGSSCNQL+AQ NASYAFNMYYQVNSQKSWNCDFDGLAVVTQQDPSYGNCQF
Sbjct: 376 LSDCTALEYGSSCNQLSAQGNASYAFNMYYQVNSQKSWNCDFDGLAVVTQQDPSYGNCQF 435

Query: 421 PIMIDYT---SSSSSFLVDERFSDLLVAVFLGIVVFFIVQYEQ 461
           PIMIDYT   SSSSSFLV ERFSDLLVAVF+GIVVFFIVQYEQ
Sbjct: 436 PIMIDYTSSSSSSSSFLVYERFSDLLVAVFMGIVVFFIVQYEQ 478

BLAST of Chy5G103960 vs. ExPASy TrEMBL
Match: A0A5A7V2U1 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold41G001360 PE=3 SV=1)

HSP 1 Score: 911.0 bits (2353), Expect = 2.0e-261
Identity = 443/462 (95.89%), Postives = 453/462 (98.05%), Query Frame = 0

Query: 1   MATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIGSDIEVMLAIPNSMLFLMSQDPH 60
           MATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIG+DIEVMLAIPNSMLFLMSQDP 
Sbjct: 1   MATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIGTDIEVMLAIPNSMLFLMSQDPQ 60

Query: 61  AAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQSLNDAGLG 120
           AAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQ+LNDAGLG
Sbjct: 61  AAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLG 120

Query: 121 SKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDLTIEILHYLSINNAPFTVNIYPFLSL 180
           SKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDL IEILHYLSINNAPFTVNIYPFLSL
Sbjct: 121 SKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDLAIEILHYLSINNAPFTVNIYPFLSL 180

Query: 181 YGNDYFPIDFAFFDGTAYRPIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVGW 240
           YGNDYFPIDFAFFDGTAY+PIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVGW
Sbjct: 181 YGNDYFPIDFAFFDGTAYKPIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVGW 240

Query: 241 PTDGDKHANIQNAKRFNQGLLRHALSGQGTPARVGIIDVYLFSLIDENAKSIEPGSFERH 300
           PTDGDKHANIQNAKRFNQGLLRHALSG+GTPARVGIID+YLFSLIDENAKSIEPGSFERH
Sbjct: 241 PTDGDKHANIQNAKRFNQGLLRHALSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERH 300

Query: 301 WGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVNDWEDLADSVDYACS 360
           WGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVNDWE LADSVDYACS
Sbjct: 301 WGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVNDWEGLADSVDYACS 360

Query: 361 LSDCTALEYGSSCNQLTAQENASYAFNMYYQVNSQKSWNCDFDGLAVVTQQDPSYGNCQF 420
           LSDCTALEYGSSCNQLTAQ NASYAFNMYYQVN+QKSWNCDFDGLAVVTQQDPSYGNC F
Sbjct: 361 LSDCTALEYGSSCNQLTAQGNASYAFNMYYQVNNQKSWNCDFDGLAVVTQQDPSYGNCLF 420

Query: 421 PIMIDYT--SSSSSFLVDERFSDLLVAVFLGIVVFFIVQYEQ 461
           PIMIDYT  SSSSSFLVDERF DLLVA+F+GIVVF +VQY+Q
Sbjct: 421 PIMIDYTSSSSSSSFLVDERFFDLLVAIFMGIVVFLMVQYDQ 462

BLAST of Chy5G103960 vs. ExPASy TrEMBL
Match: A0A5D3CH54 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G00090 PE=3 SV=1)

HSP 1 Score: 911.0 bits (2353), Expect = 2.0e-261
Identity = 443/463 (95.68%), Postives = 453/463 (97.84%), Query Frame = 0

Query: 1   MATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIGSDIEVMLAIPNSMLFLMSQDPH 60
           MATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIG+DIEVMLAIPNSMLFLMSQDP 
Sbjct: 1   MATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIGTDIEVMLAIPNSMLFLMSQDPQ 60

Query: 61  AAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQSLNDAGLG 120
           AAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQ+LNDAGLG
Sbjct: 61  AAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLG 120

Query: 121 SKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDLTIEILHYLSINNAPFTVNIYPFLSL 180
           SKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDLTIEILHYLSINNAPFTVNIYPFLSL
Sbjct: 121 SKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDLTIEILHYLSINNAPFTVNIYPFLSL 180

Query: 181 YGNDYFPIDFAFFDGTAYRPIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVGW 240
           YGNDYFPIDFAFFDGT Y+PIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVGW
Sbjct: 181 YGNDYFPIDFAFFDGTVYKPIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVGW 240

Query: 241 PTDGDKHANIQNAKRFNQGLLRHALSGQGTPARVGIIDVYLFSLIDENAKSIEPGSFERH 300
           PTDGDKHANIQNAKRFNQGLLRHALSG+GTPARVGIID+YLFSLIDENAKSIEPGSFERH
Sbjct: 241 PTDGDKHANIQNAKRFNQGLLRHALSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERH 300

Query: 301 WGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVNDWEDLADSVDYACS 360
           WGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVNDWE LADSVDYACS
Sbjct: 301 WGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVNDWEGLADSVDYACS 360

Query: 361 LSDCTALEYGSSCNQLTAQENASYAFNMYYQVNSQKSWNCDFDGLAVVTQQDPSYGNCQF 420
           LSDCTALEYGSSCNQLTAQ NASYAFNMYYQVN+QKSWNCDFDGLAVVTQQDPSYGNC F
Sbjct: 361 LSDCTALEYGSSCNQLTAQGNASYAFNMYYQVNNQKSWNCDFDGLAVVTQQDPSYGNCLF 420

Query: 421 PIMIDYT---SSSSSFLVDERFSDLLVAVFLGIVVFFIVQYEQ 461
           PIMIDYT   SSSSSFLVDERF DLLVA+F+GIVVF +VQY+Q
Sbjct: 421 PIMIDYTSSSSSSSSFLVDERFFDLLVAIFMGIVVFLMVQYDQ 463

BLAST of Chy5G103960 vs. ExPASy TrEMBL
Match: A0A1S3C742 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103497605 PE=3 SV=1)

HSP 1 Score: 910.6 bits (2352), Expect = 2.6e-261
Identity = 443/463 (95.68%), Postives = 453/463 (97.84%), Query Frame = 0

Query: 1   MATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIGSDIEVMLAIPNSMLFLMSQDPH 60
           MATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIG+DIEVMLAIPNSMLFLMSQDP 
Sbjct: 34  MATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIGTDIEVMLAIPNSMLFLMSQDPQ 93

Query: 61  AAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQSLNDAGLG 120
           AAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQ+LNDAGLG
Sbjct: 94  AAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLG 153

Query: 121 SKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDLTIEILHYLSINNAPFTVNIYPFLSL 180
           SKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDL IEILHYLSINNAPFTVNIYPFLSL
Sbjct: 154 SKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDLAIEILHYLSINNAPFTVNIYPFLSL 213

Query: 181 YGNDYFPIDFAFFDGTAYRPIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVGW 240
           YGNDYFPIDFAFFDGTAY+PIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVGW
Sbjct: 214 YGNDYFPIDFAFFDGTAYKPIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVGW 273

Query: 241 PTDGDKHANIQNAKRFNQGLLRHALSGQGTPARVGIIDVYLFSLIDENAKSIEPGSFERH 300
           PTDGDKHANIQNAKRFNQGLLRHALSG+GTPARVGIID+YLFSLIDENAKSIEPGSFERH
Sbjct: 274 PTDGDKHANIQNAKRFNQGLLRHALSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERH 333

Query: 301 WGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVNDWEDLADSVDYACS 360
           WGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVNDWE LADSVDYACS
Sbjct: 334 WGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVNDWEGLADSVDYACS 393

Query: 361 LSDCTALEYGSSCNQLTAQENASYAFNMYYQVNSQKSWNCDFDGLAVVTQQDPSYGNCQF 420
           LSDCTALEYGSSCNQLTAQ NASYAFNMYYQVN+QKSWNCDFDGLAVVTQQDPSYGNC F
Sbjct: 394 LSDCTALEYGSSCNQLTAQGNASYAFNMYYQVNNQKSWNCDFDGLAVVTQQDPSYGNCLF 453

Query: 421 PIMIDYT---SSSSSFLVDERFSDLLVAVFLGIVVFFIVQYEQ 461
           PIMIDYT   SSSSSFLVDERF DLLVA+F+GIVVF +VQY+Q
Sbjct: 454 PIMIDYTSSSSSSSSFLVDERFFDLLVAIFMGIVVFLMVQYDQ 496

BLAST of Chy5G103960 vs. ExPASy TrEMBL
Match: A0A6J1DUS6 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111024222 PE=3 SV=1)

HSP 1 Score: 843.2 bits (2177), Expect = 5.2e-241
Identity = 409/457 (89.50%), Postives = 433/457 (94.75%), Query Frame = 0

Query: 1   MATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIGSDIEVMLAIPNSMLFLMSQDPH 60
           MATHQLPPEKVVKMLQENGF KLKLFEA+DRILEALIGSDIEVMLAIPNSML +MS+DP 
Sbjct: 38  MATHQLPPEKVVKMLQENGFRKLKLFEAEDRILEALIGSDIEVMLAIPNSMLQIMSEDPE 97

Query: 61  AAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQSLNDAGLG 120
           AAASWVDSNVT Y+YHGGV IKYVAVGNEPFLKSYNGTYLQ TLPALRNIQQ+LNDAGLG
Sbjct: 98  AAASWVDSNVTTYSYHGGVNIKYVAVGNEPFLKSYNGTYLQFTLPALRNIQQALNDAGLG 157

Query: 121 SKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDLTIEILHYLSINNAPFTVNIYPFLSL 180
           SKVKATIPFNADIYNSPDSNPVPSAGEFR DVRDLTIEILH+LS NNAPFTVNIYPFLSL
Sbjct: 158 SKVKATIPFNADIYNSPDSNPVPSAGEFRSDVRDLTIEILHFLSTNNAPFTVNIYPFLSL 217

Query: 181 YGNDYFPIDFAFFDGTAYRPIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVGW 240
           Y NDYFP+DFAFFDGT Y+PI+D DL YTNVFDANFDTLVSAL KAGYP+MKIIVGEVGW
Sbjct: 218 YSNDYFPLDFAFFDGT-YKPIRDGDLAYTNVFDANFDTLVSALTKAGYPEMKIIVGEVGW 277

Query: 241 PTDGDKHANIQNAKRFNQGLLRHALSGQGTPARVGIIDVYLFSLIDENAKSIEPGSFERH 300
           PTDGDKHANIQ AKRFNQGLLRHALSG+GTPARVGIID+YLFSLIDENAKSIEPGSFERH
Sbjct: 278 PTDGDKHANIQYAKRFNQGLLRHALSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERH 337

Query: 301 WGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVNDWEDLADSVDYACS 360
           WG+FEFDGKPKYELDL+GTE+EKGL+ VEGVRYM KRWCVLNPN+NDWE LAD+VDYACS
Sbjct: 338 WGLFEFDGKPKYELDLSGTEQEKGLVAVEGVRYMVKRWCVLNPNLNDWEGLADNVDYACS 397

Query: 361 LSDCTALEYGSSCNQLTAQENASYAFNMYYQVNSQKSWNCDFDGLAVVTQQDPSYGNCQF 420
           LSDCTALEYGSSCNQLTAQ NASYAFNMYYQVNSQKSWNCDF GLAVVT++DPSYGNCQF
Sbjct: 398 LSDCTALEYGSSCNQLTAQGNASYAFNMYYQVNSQKSWNCDFSGLAVVTEEDPSYGNCQF 457

Query: 421 PIMIDYTSSSSS-FLVDERFSDLLVAVFLGIVVFFIV 457
           P+MIDYTSSSSS FLVDERF DLLVAV +G++V F V
Sbjct: 458 PVMIDYTSSSSSPFLVDERFLDLLVAVIMGLLVSFFV 493

BLAST of Chy5G103960 vs. NCBI nr
Match: XP_004139424.2 (glucan endo-1,3-beta-glucosidase 8 [Cucumis sativus] >KAE8651524.1 hypothetical protein Csa_019195 [Cucumis sativus])

HSP 1 Score: 920 bits (2379), Expect = 0.0
Identity = 453/463 (97.84%), Postives = 456/463 (98.49%), Query Frame = 0

Query: 1   MATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIGSDIEVMLAIPNSMLFLMSQDPH 60
           MATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIGSDIEVMLAIPNSMLFLMSQDPH
Sbjct: 34  MATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIGSDIEVMLAIPNSMLFLMSQDPH 93

Query: 61  AAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQSLNDAGLG 120
           AAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYL LTLPALRNIQQSLNDAGLG
Sbjct: 94  AAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLPLTLPALRNIQQSLNDAGLG 153

Query: 121 SKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDLTIEILHYLSINNAPFTVNIYPFLSL 180
           SKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDLTIEILHYLSINNAPFTVNIYPFLSL
Sbjct: 154 SKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDLTIEILHYLSINNAPFTVNIYPFLSL 213

Query: 181 YGNDYFPIDFAFFDGTAYRPIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVGW 240
           YGNDYFPIDFAFFDGTAYRPIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVGW
Sbjct: 214 YGNDYFPIDFAFFDGTAYRPIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVGW 273

Query: 241 PTDGDKHANIQNAKRFNQGLLRHALSGQGTPARVGIIDVYLFSLIDENAKSIEPGSFERH 300
           PTDGDKHAN+QNAKRFNQGLLRHALSGQGTPARVGIIDVYLFSLIDENAKSIEPGSFERH
Sbjct: 274 PTDGDKHANVQNAKRFNQGLLRHALSGQGTPARVGIIDVYLFSLIDENAKSIEPGSFERH 333

Query: 301 WGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVNDWEDLADSVDYACS 360
           WGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVNDWE LADSVDYACS
Sbjct: 334 WGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVNDWEGLADSVDYACS 393

Query: 361 LSDCTALEYGSSCNQLTAQENASYAFNMYYQVNSQKSWNCDFDGLAVVTQQDPSYGNCQF 420
           LSDCTALEYGSSCNQL+AQ NASYAFNMYYQVNSQKSWNCDFDGLAVVTQQDPSYGNCQF
Sbjct: 394 LSDCTALEYGSSCNQLSAQGNASYAFNMYYQVNSQKSWNCDFDGLAVVTQQDPSYGNCQF 453

Query: 421 PIMIDYTSSSSS---FLVDERFSDLLVAVFLGIVVFFIVQYEQ 460
           PIMIDYTSSSSS   FLV ERFSDLLVAVF+GIVVFFIVQYEQ
Sbjct: 454 PIMIDYTSSSSSSSSFLVYERFSDLLVAVFMGIVVFFIVQYEQ 496

BLAST of Chy5G103960 vs. NCBI nr
Match: TYK10700.1 (glucan endo-1,3-beta-glucosidase 8 [Cucumis melo var. makuwa])

HSP 1 Score: 907 bits (2345), Expect = 0.0
Identity = 443/463 (95.68%), Postives = 453/463 (97.84%), Query Frame = 0

Query: 1   MATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIGSDIEVMLAIPNSMLFLMSQDPH 60
           MATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIG+DIEVMLAIPNSMLFLMSQDP 
Sbjct: 1   MATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIGTDIEVMLAIPNSMLFLMSQDPQ 60

Query: 61  AAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQSLNDAGLG 120
           AAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQ+LNDAGLG
Sbjct: 61  AAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLG 120

Query: 121 SKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDLTIEILHYLSINNAPFTVNIYPFLSL 180
           SKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDLTIEILHYLSINNAPFTVNIYPFLSL
Sbjct: 121 SKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDLTIEILHYLSINNAPFTVNIYPFLSL 180

Query: 181 YGNDYFPIDFAFFDGTAYRPIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVGW 240
           YGNDYFPIDFAFFDGT Y+PIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVGW
Sbjct: 181 YGNDYFPIDFAFFDGTVYKPIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVGW 240

Query: 241 PTDGDKHANIQNAKRFNQGLLRHALSGQGTPARVGIIDVYLFSLIDENAKSIEPGSFERH 300
           PTDGDKHANIQNAKRFNQGLLRHALSG+GTPARVGIID+YLFSLIDENAKSIEPGSFERH
Sbjct: 241 PTDGDKHANIQNAKRFNQGLLRHALSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERH 300

Query: 301 WGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVNDWEDLADSVDYACS 360
           WGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVNDWE LADSVDYACS
Sbjct: 301 WGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVNDWEGLADSVDYACS 360

Query: 361 LSDCTALEYGSSCNQLTAQENASYAFNMYYQVNSQKSWNCDFDGLAVVTQQDPSYGNCQF 420
           LSDCTALEYGSSCNQLTAQ NASYAFNMYYQVN+QKSWNCDFDGLAVVTQQDPSYGNC F
Sbjct: 361 LSDCTALEYGSSCNQLTAQGNASYAFNMYYQVNNQKSWNCDFDGLAVVTQQDPSYGNCLF 420

Query: 421 PIMIDYTSSSSS---FLVDERFSDLLVAVFLGIVVFFIVQYEQ 460
           PIMIDYTSSSSS   FLVDERF DLLVA+F+GIVVF +VQY+Q
Sbjct: 421 PIMIDYTSSSSSSSSFLVDERFFDLLVAIFMGIVVFLMVQYDQ 463

BLAST of Chy5G103960 vs. NCBI nr
Match: KAA0061574.1 (glucan endo-1,3-beta-glucosidase 8 [Cucumis melo var. makuwa])

HSP 1 Score: 907 bits (2345), Expect = 0.0
Identity = 443/462 (95.89%), Postives = 453/462 (98.05%), Query Frame = 0

Query: 1   MATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIGSDIEVMLAIPNSMLFLMSQDPH 60
           MATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIG+DIEVMLAIPNSMLFLMSQDP 
Sbjct: 1   MATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIGTDIEVMLAIPNSMLFLMSQDPQ 60

Query: 61  AAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQSLNDAGLG 120
           AAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQ+LNDAGLG
Sbjct: 61  AAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLG 120

Query: 121 SKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDLTIEILHYLSINNAPFTVNIYPFLSL 180
           SKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDL IEILHYLSINNAPFTVNIYPFLSL
Sbjct: 121 SKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDLAIEILHYLSINNAPFTVNIYPFLSL 180

Query: 181 YGNDYFPIDFAFFDGTAYRPIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVGW 240
           YGNDYFPIDFAFFDGTAY+PIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVGW
Sbjct: 181 YGNDYFPIDFAFFDGTAYKPIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVGW 240

Query: 241 PTDGDKHANIQNAKRFNQGLLRHALSGQGTPARVGIIDVYLFSLIDENAKSIEPGSFERH 300
           PTDGDKHANIQNAKRFNQGLLRHALSG+GTPARVGIID+YLFSLIDENAKSIEPGSFERH
Sbjct: 241 PTDGDKHANIQNAKRFNQGLLRHALSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERH 300

Query: 301 WGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVNDWEDLADSVDYACS 360
           WGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVNDWE LADSVDYACS
Sbjct: 301 WGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVNDWEGLADSVDYACS 360

Query: 361 LSDCTALEYGSSCNQLTAQENASYAFNMYYQVNSQKSWNCDFDGLAVVTQQDPSYGNCQF 420
           LSDCTALEYGSSCNQLTAQ NASYAFNMYYQVN+QKSWNCDFDGLAVVTQQDPSYGNC F
Sbjct: 361 LSDCTALEYGSSCNQLTAQGNASYAFNMYYQVNNQKSWNCDFDGLAVVTQQDPSYGNCLF 420

Query: 421 PIMIDYTSSSSS--FLVDERFSDLLVAVFLGIVVFFIVQYEQ 460
           PIMIDYTSSSSS  FLVDERF DLLVA+F+GIVVF +VQY+Q
Sbjct: 421 PIMIDYTSSSSSSSFLVDERFFDLLVAIFMGIVVFLMVQYDQ 462

BLAST of Chy5G103960 vs. NCBI nr
Match: XP_008458070.1 (PREDICTED: glucan endo-1,3-beta-glucosidase 8 [Cucumis melo])

HSP 1 Score: 907 bits (2344), Expect = 0.0
Identity = 443/463 (95.68%), Postives = 453/463 (97.84%), Query Frame = 0

Query: 1   MATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIGSDIEVMLAIPNSMLFLMSQDPH 60
           MATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIG+DIEVMLAIPNSMLFLMSQDP 
Sbjct: 34  MATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIGTDIEVMLAIPNSMLFLMSQDPQ 93

Query: 61  AAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQSLNDAGLG 120
           AAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQ+LNDAGLG
Sbjct: 94  AAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLG 153

Query: 121 SKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDLTIEILHYLSINNAPFTVNIYPFLSL 180
           SKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDL IEILHYLSINNAPFTVNIYPFLSL
Sbjct: 154 SKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDLAIEILHYLSINNAPFTVNIYPFLSL 213

Query: 181 YGNDYFPIDFAFFDGTAYRPIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVGW 240
           YGNDYFPIDFAFFDGTAY+PIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVGW
Sbjct: 214 YGNDYFPIDFAFFDGTAYKPIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVGW 273

Query: 241 PTDGDKHANIQNAKRFNQGLLRHALSGQGTPARVGIIDVYLFSLIDENAKSIEPGSFERH 300
           PTDGDKHANIQNAKRFNQGLLRHALSG+GTPARVGIID+YLFSLIDENAKSIEPGSFERH
Sbjct: 274 PTDGDKHANIQNAKRFNQGLLRHALSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERH 333

Query: 301 WGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVNDWEDLADSVDYACS 360
           WGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVNDWE LADSVDYACS
Sbjct: 334 WGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVNDWEGLADSVDYACS 393

Query: 361 LSDCTALEYGSSCNQLTAQENASYAFNMYYQVNSQKSWNCDFDGLAVVTQQDPSYGNCQF 420
           LSDCTALEYGSSCNQLTAQ NASYAFNMYYQVN+QKSWNCDFDGLAVVTQQDPSYGNC F
Sbjct: 394 LSDCTALEYGSSCNQLTAQGNASYAFNMYYQVNNQKSWNCDFDGLAVVTQQDPSYGNCLF 453

Query: 421 PIMIDYTSSSSS---FLVDERFSDLLVAVFLGIVVFFIVQYEQ 460
           PIMIDYTSSSSS   FLVDERF DLLVA+F+GIVVF +VQY+Q
Sbjct: 454 PIMIDYTSSSSSSSSFLVDERFFDLLVAIFMGIVVFLMVQYDQ 496

BLAST of Chy5G103960 vs. NCBI nr
Match: XP_038890697.1 (glucan endo-1,3-beta-glucosidase 8-like [Benincasa hispida])

HSP 1 Score: 873 bits (2256), Expect = 0.0
Identity = 427/460 (92.83%), Postives = 443/460 (96.30%), Query Frame = 0

Query: 1   MATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIGSDIEVMLAIPNSMLFLMSQDPH 60
           MATHQLPPEKVVKMLQENGFHKLKLFEA+DRILEALIGSDIEVMLAIPNSML LMSQ+P 
Sbjct: 41  MATHQLPPEKVVKMLQENGFHKLKLFEAEDRILEALIGSDIEVMLAIPNSMLQLMSQNPR 100

Query: 61  AAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQSLNDAGLG 120
           AAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQ +LNDAGLG
Sbjct: 101 AAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQAALNDAGLG 160

Query: 121 SKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDLTIEILHYLSINNAPFTVNIYPFLSL 180
           SKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDLTIEILH+LSINNAPFTVNIYPFLSL
Sbjct: 161 SKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDLTIEILHFLSINNAPFTVNIYPFLSL 220

Query: 181 YGNDYFPIDFAFFDGTAYRPIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVGW 240
           YGNDYFP+DFAFFDGT Y+PIKD DLTYTNVFDANFDTLVSAL KAGYPDMKIIVGEVGW
Sbjct: 221 YGNDYFPMDFAFFDGT-YKPIKDGDLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEVGW 280

Query: 241 PTDGDKHANIQNAKRFNQGLLRHALSGQGTPARVGIIDVYLFSLIDENAKSIEPGSFERH 300
           PTDGDKHANIQNAKRFNQGLLRHALSG+GTPARVGIID+YLFSLIDENAKSIEPGSFERH
Sbjct: 281 PTDGDKHANIQNAKRFNQGLLRHALSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERH 340

Query: 301 WGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVNDWEDLADSVDYACS 360
           WGIFEFDGKPKYELDL+GTEEEKGLIPVEGVRYM KRWC+LNPNVNDWE LADS+DYACS
Sbjct: 341 WGIFEFDGKPKYELDLSGTEEEKGLIPVEGVRYMVKRWCILNPNVNDWEGLADSIDYACS 400

Query: 361 LSDCTALEYGSSCNQLTAQENASYAFNMYYQVNSQKSWNCDFDGLAVVTQQDPSYGNCQF 420
           LSDCTALEYGSSCNQLTAQ NASYAFNMYYQVNSQKSWNCDFDGLAVVTQQDPSYGNCQF
Sbjct: 401 LSDCTALEYGSSCNQLTAQGNASYAFNMYYQVNSQKSWNCDFDGLAVVTQQDPSYGNCQF 460

Query: 421 PIMIDYTSSS-SSFLVDERFSDLLVAVFLGIVVFFIVQYE 459
           PIMIDY SSS S FL DERF DLLV VF+G +VF I++Y+
Sbjct: 461 PIMIDYASSSYSPFLFDERFLDLLVEVFMGFMVFLILEYD 499

BLAST of Chy5G103960 vs. TAIR 10
Match: AT3G24330.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 616.7 bits (1589), Expect = 1.5e-176
Identity = 294/457 (64.33%), Postives = 371/457 (81.18%), Query Frame = 0

Query: 1   MATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIGSDIEVMLAIPNSMLFLMSQDPH 60
           MA+HQLPPEKVVKML +N F KLKLFEAD  IL+ALIGSDIEVM+ IPN  L  M+QD  
Sbjct: 46  MASHQLPPEKVVKMLMDNSFTKLKLFEADQNILDALIGSDIEVMIGIPNRFLKEMAQDTS 105

Query: 61  AAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQSLNDAGLG 120
            AASWV+ NVTAY+Y+GGV IKY+AVGNEPFL++YNGTY++ TLPAL NIQ++L +A L 
Sbjct: 106 VAASWVEENVTAYSYNGGVNIKYIAVGNEPFLQTYNGTYVEFTLPALINIQRALEEADL- 165

Query: 121 SKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDLTIEILHYLSINNAPFTVNIYPFLSL 180
             VK T+PFNADIY SP++NPVPSAG+FRP++RD TIEI+++L  +++PFTVNIYPFLSL
Sbjct: 166 KNVKVTVPFNADIYFSPEANPVPSAGDFRPELRDATIEIINFLYSHDSPFTVNIYPFLSL 225

Query: 181 YGNDYFPIDFAFFDGTAYRPIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVGW 240
           YGN YFP+DFAFFDGT  + ++D +L YTNVFDAN DTL+ A+ +  +  MKIIVGEVGW
Sbjct: 226 YGNAYFPLDFAFFDGT-NKSLRDGNLVYTNVFDANLDTLICAMERYSFLGMKIIVGEVGW 285

Query: 241 PTDGDKHANIQNAKRFNQGLLRHALSGQGTPARVGII-DVYLFSLIDENAKSIEPGSFER 300
           PTDGDK+AN+++AKRFNQG+++HA+SG GTPAR G+I DVYLFSL+DE+AKSI PG+FER
Sbjct: 286 PTDGDKNANVKSAKRFNQGMVKHAMSGNGTPARKGVIMDVYLFSLVDEDAKSIAPGTFER 345

Query: 301 HWGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVNDWEDLADSVDYAC 360
           HWGIFEFDG+PKYELDL+G   +K L+PVE V+Y+ K WC+L+PN  + +DL D++DYAC
Sbjct: 346 HWGIFEFDGRPKYELDLSGKGNDKPLVPVEDVKYLPKTWCILDPNAYNLDDLPDNIDYAC 405

Query: 361 SLSDCTALEYGSSCNQLTAQENASYAFNMYYQVNSQKSWNCDFDGLAVVTQQDPSYGNCQ 420
           SLSDCTAL YGSSCN LTA  N SYAFNMYYQ++ QK+W+CDF GL ++T +DPS   C+
Sbjct: 406 SLSDCTALGYGSSCNHLTATGNVSYAFNMYYQMHDQKTWDCDFLGLGLITDEDPSDELCE 465

Query: 421 FPIMIDYTSSSSSFLVDERFSDLLVAVFLGIVVFFIV 457
           FP+MID   +  S  +    S +L  V   ++V  ++
Sbjct: 466 FPVMID---TGDSTRLQPGSSRVLTRVAAAVLVMLVL 497

BLAST of Chy5G103960 vs. TAIR 10
Match: AT2G19440.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 530.0 bits (1364), Expect = 1.9e-150
Identity = 260/450 (57.78%), Postives = 325/450 (72.22%), Query Frame = 0

Query: 1   MATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIGSDIEVMLAIPNSMLFLMSQDPH 60
           MATH+LPP+KVV+ML++N  +K+KLF+AD+  + AL GS +EVM+AIPN  L +M     
Sbjct: 28  MATHKLPPKKVVQMLKDNNINKVKLFDADETTMSALSGSGLEVMVAIPNDQLKVMGSYDR 87

Query: 61  AAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQSLNDAGLG 120
            A  WV  NVT Y ++GGV I +VAVGNEPFLKSYNG+++ LT PAL+NIQ +LN+AGLG
Sbjct: 88  -AKDWVHKNVTRYNFNGGVNITFVAVGNEPFLKSYNGSFINLTFPALQNIQNALNEAGLG 147

Query: 121 SKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDLTIEILHYLSINNAPFTVNIYPFLSL 180
           S VKAT+P NAD+Y+SP SNPVPSAG FRPD+     +I+ +L  N+AP T+NIYPFLSL
Sbjct: 148 SSVKATVPLNADVYDSPSSNPVPSAGRFRPDIIGQMTQIVDFLGNNSAPITINIYPFLSL 207

Query: 181 YGNDYFPIDFAFFDGTAYRPIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVGW 240
           YGND FP+++AFFDG   +P+ D  + YTNVFDANFDTLVSAL   G+ DM IIVGEVGW
Sbjct: 208 YGNDDFPLNYAFFDGA--KPVDDNGIAYTNVFDANFDTLVSALKAVGHGDMPIIVGEVGW 267

Query: 241 PTDGDKHANIQNAKRFNQGLLRHALSGQGTPARVGIIDVYLFSLIDENAKSIEPGSFERH 300
           PT+GDKHAN  +A RF  GLL      +GTP R   I+VYLF L+DE+AKSI PG FERH
Sbjct: 268 PTEGDKHANSGSAYRFYNGLLPRLGENRGTPLRPTYIEVYLFGLLDEDAKSIAPGEFERH 327

Query: 301 WGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVNDWEDLADSVDYACS 360
           WGIF+FDG+PK+ +DL+G  + K LI  E V Y  K+WC+ N    D   LA ++DYAC+
Sbjct: 328 WGIFKFDGQPKFPIDLSGQGQNKLLIGAENVTYQPKKWCMFNTEAKDLTKLAANIDYACT 387

Query: 361 LSDCTALEYGSSCNQLTAQENASYAFNMYYQVNSQKSWNCDFDGLAVVTQQDPSYGNCQF 420
            SDCTAL YGSSCN L A  NASYAFNMY+QV +Q    C F GLA +T ++ S G C F
Sbjct: 388 FSDCTALGYGSSCNTLDANGNASYAFNMYFQVKNQDEDACIFQGLATITTKNISQGQCNF 447

Query: 421 PIMIDYTSSSSSFLVDERFSDLLVAVFLGI 451
           PI I  +++SS       FS  LV +  G+
Sbjct: 448 PIQIVASTASS-------FSSSLVLLIAGV 467

BLAST of Chy5G103960 vs. TAIR 10
Match: AT1G64760.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 526.2 bits (1354), Expect = 2.7e-149
Identity = 259/448 (57.81%), Postives = 322/448 (71.88%), Query Frame = 0

Query: 1   MATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIGSDIEVMLAIPNSMLFLMSQDPH 60
           MATH+LPP+ VV+ML++N  +K+KLF+AD+  + AL GS +EVM+AIPN  L +M+    
Sbjct: 32  MATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGSGLEVMVAIPNDQLKVMTSYDR 91

Query: 61  AAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQSLNDAGLG 120
            A  WV  NVT Y + GGV I +VAVGNEPFLKSYNG+++ LT PAL NIQ +LN+AGLG
Sbjct: 92  -AKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFINLTFPALANIQNALNEAGLG 151

Query: 121 SKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDLTIEILHYLSINNAPFTVNIYPFLSL 180
           + VKAT+P NAD+Y+SP SNPVPSAG FRPD+     +I+ +L  NNAP T+NIYPFLSL
Sbjct: 152 NSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIVDFLGKNNAPITINIYPFLSL 211

Query: 181 YGNDYFPIDFAFFDGTAYRPIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVGW 240
           YGND FP+++AFFDG    PI D  + YTNVFDANFDTLVS+L   G+ DM IIVGEVGW
Sbjct: 212 YGNDDFPLNYAFFDGA--EPINDNGIDYTNVFDANFDTLVSSLKAVGHGDMPIIVGEVGW 271

Query: 241 PTDGDKHANIQNAKRFNQGLLRHALSGQGTPARVGIIDVYLFSLIDENAKSIEPGSFERH 300
           PT+GDKHAN  +A RF  GLL    + +GTP R   I+VYLF L+DE+AKSI PG FERH
Sbjct: 272 PTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPTYIEVYLFGLLDEDAKSIAPGPFERH 331

Query: 301 WGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVNDWEDLADSVDYACS 360
           WGIF+FDG+PK+ +DL+G  + K LI  + V Y+  +WC  NP   D   LA ++DYAC+
Sbjct: 332 WGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWCTFNPEAKDLTKLAANIDYACT 391

Query: 361 LSDCTALEYGSSCNQLTAQENASYAFNMYYQVNSQKSWNCDFDGLAVVTQQDPSYGNCQF 420
            SDCTAL YGSSCN L A  NASYAFNM++QV +Q    C F GLA +T Q+ S G C F
Sbjct: 392 FSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESACYFQGLATITTQNISQGQCNF 451

Query: 421 PIMIDYTSSSSSFLVDERFSDLLVAVFL 449
           PI I   SS+SSF        +L+  FL
Sbjct: 452 PIQI-VASSASSFSCSSYSLVVLIVWFL 475

BLAST of Chy5G103960 vs. TAIR 10
Match: AT1G64760.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 526.2 bits (1354), Expect = 2.7e-149
Identity = 259/448 (57.81%), Postives = 322/448 (71.88%), Query Frame = 0

Query: 1   MATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIGSDIEVMLAIPNSMLFLMSQDPH 60
           MATH+LPP+ VV+ML++N  +K+KLF+AD+  + AL GS +EVM+AIPN  L +M+    
Sbjct: 32  MATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGSGLEVMVAIPNDQLKVMTSYDR 91

Query: 61  AAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQSLNDAGLG 120
            A  WV  NVT Y + GGV I +VAVGNEPFLKSYNG+++ LT PAL NIQ +LN+AGLG
Sbjct: 92  -AKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFINLTFPALANIQNALNEAGLG 151

Query: 121 SKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDLTIEILHYLSINNAPFTVNIYPFLSL 180
           + VKAT+P NAD+Y+SP SNPVPSAG FRPD+     +I+ +L  NNAP T+NIYPFLSL
Sbjct: 152 NSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIVDFLGKNNAPITINIYPFLSL 211

Query: 181 YGNDYFPIDFAFFDGTAYRPIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVGW 240
           YGND FP+++AFFDG    PI D  + YTNVFDANFDTLVS+L   G+ DM IIVGEVGW
Sbjct: 212 YGNDDFPLNYAFFDGA--EPINDNGIDYTNVFDANFDTLVSSLKAVGHGDMPIIVGEVGW 271

Query: 241 PTDGDKHANIQNAKRFNQGLLRHALSGQGTPARVGIIDVYLFSLIDENAKSIEPGSFERH 300
           PT+GDKHAN  +A RF  GLL    + +GTP R   I+VYLF L+DE+AKSI PG FERH
Sbjct: 272 PTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPTYIEVYLFGLLDEDAKSIAPGPFERH 331

Query: 301 WGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVNDWEDLADSVDYACS 360
           WGIF+FDG+PK+ +DL+G  + K LI  + V Y+  +WC  NP   D   LA ++DYAC+
Sbjct: 332 WGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWCTFNPEAKDLTKLAANIDYACT 391

Query: 361 LSDCTALEYGSSCNQLTAQENASYAFNMYYQVNSQKSWNCDFDGLAVVTQQDPSYGNCQF 420
            SDCTAL YGSSCN L A  NASYAFNM++QV +Q    C F GLA +T Q+ S G C F
Sbjct: 392 FSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESACYFQGLATITTQNISQGQCNF 451

Query: 421 PIMIDYTSSSSSFLVDERFSDLLVAVFL 449
           PI I   SS+SSF        +L+  FL
Sbjct: 452 PIQI-VASSASSFSCSSYSLVVLIVWFL 475

BLAST of Chy5G103960 vs. TAIR 10
Match: AT3G04010.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 492.3 bits (1266), Expect = 4.3e-139
Identity = 237/430 (55.12%), Postives = 310/430 (72.09%), Query Frame = 0

Query: 1   MATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIGSDIEVMLAIPNSMLFLMSQDPH 60
           MA+HQLPP+ VV+ML++N   K+KLF+AD   + AL GS +EVM+AIPN +L  M  +  
Sbjct: 40  MASHQLPPKTVVEMLKDNNIQKVKLFDADTNTMGALAGSGVEVMVAIPNDLLLAMG-NYQ 99

Query: 61  AAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQSLNDAGLG 120
            A  WV  NV+ + ++ GVKIKYVAVGNEPFL +YNG+++ LT PAL NIQ +LN+AG+G
Sbjct: 100 RAKDWVQRNVSRFNFNNGVKIKYVAVGNEPFLTAYNGSFINLTYPALFNIQTALNEAGVG 159

Query: 121 SKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDLTIEILHYLSINNAPFTVNIYPFLSL 180
              KAT+P NAD+YNSP  N VPSAG FR D+     +I+++L+ N APFTVNIYPFLSL
Sbjct: 160 DFTKATVPLNADVYNSPPDNQVPSAGRFRSDIIQEMTQIVNFLAQNKAPFTVNIYPFLSL 219

Query: 181 YGNDYFPIDFAFFDGTAYRPIKDRDLTYTNVFDANFDTLVSALAKAGYPDMKIIVGEVGW 240
           Y +  FP ++AFFDG     + D  + YTNVFDA+FDTL+++L    + +M++IVGEVGW
Sbjct: 220 YLSSDFPFEYAFFDG--QNTVNDNGVIYTNVFDASFDTLLASLNALNHGNMEVIVGEVGW 279

Query: 241 PTDGDKHANIQNAKRFNQGLLRHALSGQGTPARVGIIDVYLFSLIDENAKSIEPGSFERH 300
           PTDGDK+AN+ NA+RF  GLL    +  GTP R G I+VYLF  IDE+AKS+ PG+FERH
Sbjct: 280 PTDGDKNANVPNAERFYSGLLPRLANNVGTPMRKGYIEVYLFGFIDEDAKSVAPGNFERH 339

Query: 301 WGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPN-VNDWEDLADSVDYAC 360
           WGIF+FDG+PK+ +D  G  ++K L   + V+Y   +WC+ NPN   +   L D+++YAC
Sbjct: 340 WGIFKFDGQPKFPVDFRGQGQKKFLTGAQNVQYFLNQWCMFNPNGRGNMSRLGDNINYAC 399

Query: 361 SLSDCTALEYGSSCNQLTAQENASYAFNMYYQVNSQKSWNCDFDGLAVVTQQDPSYGNCQ 420
           S SDCTAL YGSSC  L A  NASYAFNMY+QV +Q++  CDF+GLA +T Q+ S G C 
Sbjct: 400 SHSDCTALGYGSSCGNLDANGNASYAFNMYFQVQNQEAQACDFEGLATITTQNISQGQCN 459

Query: 421 FPIMIDYTSS 430
           FPI I   SS
Sbjct: 460 FPIQIGEPSS 466

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6NKW93.8e-14857.81Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana OX=3702 GN=At1g64760 ... [more]
Q9M0881.8e-13451.75Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana OX=3702 GN=At4g31140 ... [more]
Q93Z085.3e-13455.53Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana OX=3702 GN=At5g58090 ... [more]
Q9FGH47.3e-9141.44Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana OX=3702 GN=At5g58480 ... [more]
Q9M0691.2e-6134.67Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ... [more]
Match NameE-valueIdentityDescription
A0A0A0LHZ12.3e-26597.84(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_2G005360 PE=3... [more]
A0A5A7V2U12.0e-26195.89(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A5D3CH542.0e-26195.68(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3C7422.6e-26195.68(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103497605 PE=3 SV... [more]
A0A6J1DUS65.2e-24189.50(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111024222 ... [more]
Match NameE-valueIdentityDescription
XP_004139424.20.097.84glucan endo-1,3-beta-glucosidase 8 [Cucumis sativus] >KAE8651524.1 hypothetical ... [more]
TYK10700.10.095.68glucan endo-1,3-beta-glucosidase 8 [Cucumis melo var. makuwa][more]
KAA0061574.10.095.89glucan endo-1,3-beta-glucosidase 8 [Cucumis melo var. makuwa][more]
XP_008458070.10.095.68PREDICTED: glucan endo-1,3-beta-glucosidase 8 [Cucumis melo][more]
XP_038890697.10.092.83glucan endo-1,3-beta-glucosidase 8-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT3G24330.11.5e-17664.33O-Glycosyl hydrolases family 17 protein [more]
AT2G19440.11.9e-15057.78O-Glycosyl hydrolases family 17 protein [more]
AT1G64760.12.7e-14957.81O-Glycosyl hydrolases family 17 protein [more]
AT1G64760.22.7e-14957.81O-Glycosyl hydrolases family 17 protein [more]
AT3G04010.14.3e-13955.12O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012946X8 domainSMARTSM00768X8_clscoord: 337..420
e-value: 2.5E-37
score: 140.0
IPR012946X8 domainPFAMPF07983X8coord: 338..406
e-value: 9.0E-19
score: 67.9
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 1..323
e-value: 4.4E-105
score: 353.2
NoneNo IPR availableGENE3D1.20.58.1040coord: 333..429
e-value: 9.8E-10
score: 40.7
NoneNo IPR availablePANTHERPTHR32227:SF319BETA-1,3-GLUCANASE-LIKE PROTEINcoord: 1..431
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 8..315
e-value: 5.9E-64
score: 216.6
IPR000490Glycoside hydrolase family 17PROSITEPS00587GLYCOSYL_HYDROL_F17coord: 231..244
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 1..431
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 4..315

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy5G103960.1Chy5G103960.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds