Chy5G103660 (gene) Cucumber (hystrix) v1

Overview


Name	Chy5G103660
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Pectin lyase-like superfamily protein
Location	chrH05: 17351966 .. 17354420 (+)
RNA-Seq Expression	Chy5G103660
Synteny	Chy5G103660

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTCGTGAAAAGGTTTCCAGAAGAGGCCATCTTTAATGTCATGAGTTATGGAGCTGTGGGAGGAAGCAACACAGATAGTTCTCAAGTAAAATCTATCTGTGGATTTGCAGTTCTGTATGGGATCTGTTAACTACATTACCCTTCAATTGAAAGAGCTGGGAGTGTGGAGTGATTGATTCTTACACCACAATAGTGCACCTTAGCCGAGTGGTCAAATGTGTGAGAGCCAAGTTAGAGATGTAATAGAATTATTAGAAGATGTGAGCCCAATAGGGTTATCAGTAGAATATTAGTATGGTTATTAGAAAGGGTATAATATTAGTATGATTATTAGAAGATGTGAGCCCCATAGGTAGTAGGTTTGTTAGGATTTTAGCTATAAATAGAGGGAGTTGGTTGGGAGCAAGGGTGTGAAGAGTTTTGTAGTGATTTCCCTGGGAAATTTTGGGAAAGAGTAGCCCTCTTAAAAGGCTATAGTATATTGTGAATATACTGTAGTATATTGTAGGTTCGTTGATATTGTAATATACTAATATTAGTCTACCTTTTAGTGTTCTTTGTTGTTCTTGAGTGTTCTTGTTATGTGGTCTCTTCATTGTATCCTAAAATCCTACTAATATTCCTAATATGTCCCCAACTAACATGGAATATAACTCATGTTTTGGCAGGCAATTTCAAAAGCATGGAAGGCAGCCTGTAATTCTAAAACAAAAAGTTCTACGGTGCTAATTCCCAAGAGGCAAACAATTATGGTAAAACCAATTCTTTTGAGTGGTCCTTGCAGAGCAAAAAGCATCACTTTCATGGTAAAGAAAAAGCAGAGATTTTCCCTTTATGATTAAATCTTTCCTAGAATACTACCTGTCTTCAAGTAGATGCAAGTTGTCACAGATAAATGGAGTGATTGTGGCACCAACCTCACCAAAGGGATGGGCGGGAACTGACAAGAGTCTGGCTAGCTTTCGAAGGGATAAGCAGGCTCAGTATCACTGGCTCTGGCATCGGCAAGATTGATGGACGAGGTACAGGCTGGTGGAACCAATCATGCAGGGATCACCCAAACTTGGTAAGAGTGAATCAGAAAGGAAATTTTCAAAATGCAAATTAAGCCAAATATCTGATGCTTCTTCCACTTTTGGCAGGAGAGCTGCACCTCAATAGCACCTACTGTAAGTATCCTGCACAAAGCAATTTTATTAACAAACAGATGGTTTCTTGATAAAAACCTCGACCAAATAGATGAATTTACCTGAGAGAATCTAGAGCCACTACTACAGGCGATGAAGATCATTTCATGCAACGACACCAACCTGAGCAACCTTCACTTTATTAACAGTTCACAAGCACATGTACTATTAATGGGGTTCAGAAGAACCGAAATTAAGAACTTGGTAATCACAGCTCCTGAAAGCAGTCCAAACACTGACGGGATTCACATTCATTCTTCTCACAATATTATTATAAGGAACGCAGCTATAGGAACAGGTATCTTATGGAATCATTTCCTCGAACATCAATTTTACTAAGATTAATAAGTGTTTAATTGAGACAACAAATAAGTAATTGTGATGAACAGGTGATGATTGTGCATCTATAGGAGATTACACCTCCCATGTGAATATCTCACATATTAAATGTGGACCTGGTCATGGAATAAGGTAGGCTCCCTATTCAGATAAACTCTAGAGAATCACTTTTAGAGCCAAAATGAAAACAAAGAAATTAGAACAAAATTCGATATTCCAATATCTGATTTCATATGCTGTCTGGTAATAGTATTGGAAGTTTGGGTAGAGGTGGCAATTTTGTACAAGTGGAGGACATTAGCGTGAGTAATATCTACTTCAAAGGGACTACCAATGGAGCTCGGATTAAGACTTGGCAGGTTAATGGAAATTCTAAATAATTTACATCTCAATAAAAATAAACTATAAAGCTTCCACTAACACTTCCAAAAGATTGGTAGGGGTACCATTCAAAGGGTCACATTTGAGAATCTCCATTTTGAATCAGTCAATAACCCCTTAATTATAGACCAAAACTATGGTAAGGAAGAGGAATTTTGCAAGGAAAAGGTAAGAGAAACCGAACTTCTTTGCGATACTCATTTTAAATTTTCAGCTTTGAATTATTCAGCATATAGTGCTCATAAATTTTTTATATTTTCACAGCAATCAGGCGTTCATATAGCCAATGTATCCATCAAAAACCTTTTTGGAACATCAATGAGCAATGTGGCTATCAATCTCAATTGCAGTAGGTCTGTTGCCTGCACTGGTATACTGTTGAATTCAATTCAGCTTCAATCTGTAAACAGTGGACAAAAGGTGACCCAAGTTGCATCAATGCCCATGGATTTGCCTTTGGAGTGGTTCAACCTAAATCCTGTCTTCAGATCTAAATCTGAATCCTACAAAGATCCTACCCCTCCTTATTTAGCAAGGGGTAGATGA

mRNA sequence

ATGTTCGTGAAAAGGTTTCCAGAAGAGGCCATCTTTAATGTCATGAGTTATGGAGCTGTGGGAGGAAGCAACACAGATAGTTCTCAAGTAAAATCTATCTGTGGATTTGCAGTTCTGGATGGGCGGGAACTGACAAGAGTCTGGCTAGCTTTCGAAGGGATAAGCAGGCTCAGTATCACTGGCTCTGGCATCGGCAAGATTGATGGACGAGGTACAGGCTGGTGGAACCAATCATGCAGGGATCACCCAAACTTGGAGAGCTGCACCTCAATAGCACCTACTGCGATGAAGATCATTTCATGCAACGACACCAACCTGAGCAACCTTCACTTTATTAACAGTTCACAAGCACATGTACTATTAATGGGGTTCAGAAGAACCGAAATTAAGAACTTGGTAATCACAGCTCCTGAAAGCAGTCCAAACACTGACGGGATTCACATTCATTCTTCTCACAATATTATTATAAGGAACGCAGCTATAGGAACAGGTGATGATTGTGCATCTATAGGAGATTACACCTCCCATGTGAATATCTCACATATTAAATGTGGACCTGGTCATGGAATAAGTATTGGAAGTTTGGGTAGAGGTGGCAATTTTGTACAAGTGGAGGACATTAGCGTGAGTAATATCTACTTCAAAGGGACTACCAATGGAGCTCGGATTAAGACTTGGCAGCAATCAGGCGTTCATATAGCCAATGTATCCATCAAAAACCTTTTTGGAACATCAATGAGCAATGTGGCTATCAATCTCAATTGCAGTAGGTCTGTTGCCTGCACTGGTATACTGTTGAATTCAATTCAGCTTCAATCTGTAAACAGTGGACAAAAGGTGACCCAAGTTGCATCAATGCCCATGGATTTGCCTTTGGAGTGGTTCAACCTAAATCCTGTCTTCAGATCTAAATCTGAATCCTACAAAGATCCTACCCCTCCTTATTTAGCAAGGGGTAGATGA

Coding sequence (CDS)

ATGTTCGTGAAAAGGTTTCCAGAAGAGGCCATCTTTAATGTCATGAGTTATGGAGCTGTGGGAGGAAGCAACACAGATAGTTCTCAAGTAAAATCTATCTGTGGATTTGCAGTTCTGGATGGGCGGGAACTGACAAGAGTCTGGCTAGCTTTCGAAGGGATAAGCAGGCTCAGTATCACTGGCTCTGGCATCGGCAAGATTGATGGACGAGGTACAGGCTGGTGGAACCAATCATGCAGGGATCACCCAAACTTGGAGAGCTGCACCTCAATAGCACCTACTGCGATGAAGATCATTTCATGCAACGACACCAACCTGAGCAACCTTCACTTTATTAACAGTTCACAAGCACATGTACTATTAATGGGGTTCAGAAGAACCGAAATTAAGAACTTGGTAATCACAGCTCCTGAAAGCAGTCCAAACACTGACGGGATTCACATTCATTCTTCTCACAATATTATTATAAGGAACGCAGCTATAGGAACAGGTGATGATTGTGCATCTATAGGAGATTACACCTCCCATGTGAATATCTCACATATTAAATGTGGACCTGGTCATGGAATAAGTATTGGAAGTTTGGGTAGAGGTGGCAATTTTGTACAAGTGGAGGACATTAGCGTGAGTAATATCTACTTCAAAGGGACTACCAATGGAGCTCGGATTAAGACTTGGCAGCAATCAGGCGTTCATATAGCCAATGTATCCATCAAAAACCTTTTTGGAACATCAATGAGCAATGTGGCTATCAATCTCAATTGCAGTAGGTCTGTTGCCTGCACTGGTATACTGTTGAATTCAATTCAGCTTCAATCTGTAAACAGTGGACAAAAGGTGACCCAAGTTGCATCAATGCCCATGGATTTGCCTTTGGAGTGGTTCAACCTAAATCCTGTCTTCAGATCTAAATCTGAATCCTACAAAGATCCTACCCCTCCTTATTTAGCAAGGGGTAGATGA

Protein sequence

MFVKRFPEEAIFNVMSYGAVGGSNTDSSQVKSICGFAVLDGRELTRVWLAFEGISRLSITGSGIGKIDGRGTGWWNQSCRDHPNLESCTSIAPTAMKIISCNDTNLSNLHFINSSQAHVLLMGFRRTEIKNLVITAPESSPNTDGIHIHSSHNIIIRNAAIGTGDDCASIGDYTSHVNISHIKCGPGHGISIGSLGRGGNFVQVEDISVSNIYFKGTTNGARIKTWQQSGVHIANVSIKNLFGTSMSNVAINLNCSRSVACTGILLNSIQLQSVNSGQKVTQVASMPMDLPLEWFNLNPVFRSKSESYKDPTPPYLARGR*

Homology

[Close]

BLAST of Chy5G103660 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 1.1e-45
Identity = 105/269 (39.03%), Postives = 140/269 (52.04%), Query Frame = 0


Query: 46  RVWLAFEGISRLSITGSGIGKIDGRGTGWWNQSCRDHPNLESCTSIAPTAMKIISCNDTN 105
           R WL F G+SRL++ G   G ++G G  WW +SC+ H +   C   APTA+    C +  
Sbjct: 134 RKWLYFHGLSRLTVEGG--GTVNGMGQEWWERSCK-HNHSNPCRG-APTALTFHKCKNMR 193

Query: 106 LSNLHFINSSQAHVLLMGFRRTEIKNLVITAPESSPNTDGIHIHSSHNIIIRNAAIGTGD 165
           + NL+ I+S Q H+ L   RR  I  L + AP +SPNTDGIHI  S  I+I N  + TGD
Sbjct: 194 VENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNTDGIHISVSRGIVIDNTTVSTGD 253

Query: 166 DCASIGDYTSHVNISHIKCGPGHGISIGSLGRGGNFVQVEDISVSNIYFKGTTNGARIKT 225
           DC SI   ++ ++IS+I CGPGHGISIGSLG+  ++ +V DI+V       T NG RIKT
Sbjct: 254 DCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEEVRDITVDTAIISDTANGVRIKT 313

Query: 226 W---------------------------------------QQSGVHIANVSIKNLFGTSM 276
           W                                       Q S + I N+S  ++ GTS 
Sbjct: 314 WQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPCANQTSAISIENISFVHVRGTSA 373

[Close]

BLAST of Chy5G103660 vs. ExPASy Swiss-Prot
Match: P48978 (Polygalacturonase OS=Malus domestica OX=3750 PE=2 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 1.4e-45
Identity = 104/266 (39.10%), Postives = 138/266 (51.88%), Query Frame = 0


Query: 48  WLAFEGISRLSITGSGIGKIDGRGTGWWNQSCRDHPNLESCTSIAPTAMKIISCNDTNLS 107
           WL F+ +  L + G   G I+G G  WW  SC+  P    C + APTA+    CN+  + 
Sbjct: 178 WLIFDNVQNLLVVGP--GTINGNGNIWWKNSCKIKPQ-PPCGTYAPTAVTFNRCNNLVVK 237

Query: 108 NLHFINSSQAHVLLMGFRRTEIKNLVITAPESSPNTDGIHIHSSHNIIIRNAAIGTGDDC 167
           NL+  ++ Q HV+       +   L +TAPE SPNTDGIH+ ++ NI I ++ IGTGDDC
Sbjct: 238 NLNIQDAQQIHVIFQNCINVQASCLTVTAPEDSPNTDGIHVTNTQNITISSSVIGTGDDC 297

Query: 168 ASIGDYTSHVNISHIKCGPGHGISIGSLGRGGNFVQVEDISVSNIYFKGTTNGARIKTW- 227
            SI   +  V  + I CGPGHGISIGSLG  G+   V  + V+     GT+NG RIKTW 
Sbjct: 298 ISIVSGSQRVQATDITCGPGHGISIGSLGEDGSEDHVSGVFVNGAKLSGTSNGLRIKTWK 357

Query: 228 ---------------------------------------QQSGVHIANVSIKNLFGTSMS 274
                                                  Q+S V + NV  +N+ GTS S
Sbjct: 358 GGSGSATNIVFQNVQMNDVTNPIIIDQNYCDHKTKDCKQQKSAVQVKNVLYQNIRGTSAS 417

[Close]

BLAST of Chy5G103660 vs. ExPASy Swiss-Prot
Match: Q9SFB7 (Polygalacturonase QRT2 OS=Arabidopsis thaliana OX=3702 GN=QRT2 PE=1 SV=2)

HSP 1 Score: 179.1 bits (453), Expect = 7.9e-44
Identity = 95/265 (35.85%), Postives = 142/265 (53.58%), Query Frame = 0


Query: 46  RVWLAFEGISRLSITGSGIGKIDGRGTGWWNQSCRDHPNLESCTSIAPTAMKIISCNDTN 105
           R W+ FE ++ L + G   G+IDG G  WW +SC+ +P L  C   APTA+  + CN+  
Sbjct: 150 RHWIVFENVNNLRVEGG--GRIDGNGHIWWPKSCKINPQL-PCLG-APTAVTFVECNNLR 209

Query: 106 LSNLHFINSSQAHVLLMGFRRTEIKNLVITAPESSPNTDGIHIHSSHNIIIRNAAIGTGD 165
           +SN+   N+ Q H+     +  +  NL++T+P  SPNTDGIH+  + NI+I+++ + TGD
Sbjct: 210 VSNIRLENAQQMHLTFQDCKNVKALNLMVTSPADSPNTDGIHVSGTQNILIQDSIVRTGD 269

Query: 166 DCASIGDYTSHVNISHIKCGPGHGISIGSLGRGGNFVQVEDISVSNIYFKGTTNGARIKT 225
           DC SI   + +V  + I CGPGHGISIGSLG   +   V ++ V+     GTTNG RIKT
Sbjct: 270 DCISIVSGSENVRATGITCGPGHGISIGSLGEDNSEAYVSNVVVNKATLIGTTNGVRIKT 329

Query: 226 W---------------------------------------QQSGVHIANVSIKNLFGTSM 272
           W                                       Q+S V ++NV  KN+ GTS 
Sbjct: 330 WQGGHGMAKNIIFQDIIMKNVTNPIIINQDYCDRVEACPEQKSAVQVSNVLYKNIQGTSS 389

[Close]

BLAST of Chy5G103660 vs. ExPASy Swiss-Prot
Match: O23147 (Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 1.0e-43
Identity = 102/274 (37.23%), Postives = 142/274 (51.82%), Query Frame = 0


Query: 48  WLAFEGISRLSITGSGIGKIDGRGTGWWNQSCRDHPNLESCTSIAPTAMKIISCNDTNLS 107
           WL  E ++ LSI G   G I+G G  WW  SC+   + + CT  APTA+ + +  + N+ 
Sbjct: 149 WLILEDVNNLSIDGGSTGIINGNGKTWWQNSCKIDKS-KPCTK-APTALTLYNLKNLNVK 208

Query: 108 NLHFINSSQAHVLLMGFRRTEIKNLVITAPESSPNTDGIHIHSSHNIIIRNAAIGTGDDC 167
           NL   N+ Q  + +    + E+ N+ ITAP  SPNTDGIHI ++ NI + N+ IGTGDDC
Sbjct: 209 NLRVKNAQQIQISIEKCNKVEVSNVEITAPGDSPNTDGIHITNTQNIRVSNSDIGTGDDC 268

Query: 168 ASIGDYTSHVNISHIKCGPGHGISIGSLGRGGNFVQVEDISVSNIYFKGTTNGARIKTW- 227
            SI D T ++ I  + CGPGHGISIGSLG   +   V  I+V    F  + NG RIKT+ 
Sbjct: 269 ISIEDGTQNLQIFDLTCGPGHGISIGSLGDDNSKAYVSGINVDGAKFSESDNGVRIKTYQ 328

Query: 228 -------------------------------------QQSGVHIANVSIKNLFGTSMSNV 284
                                                Q+S V + NV  KN+ GTS ++V
Sbjct: 329 GGSGTAKNIKFQNIRMENVKNPIIIDQDYCDKDKCEDQESAVQVKNVVYKNISGTSATDV 388

[Close]

BLAST of Chy5G103660 vs. ExPASy Swiss-Prot
Match: Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 1.8e-43
Identity = 101/271 (37.27%), Postives = 137/271 (50.55%), Query Frame = 0


Query: 48  WLAFEGISRLSITGSGIGKIDGRGTGWWNQSCRDHPNLESCTSIAPTAMKIISCNDTNLS 107
           W+ F  I  L I G   G+I+G+G+ WW                 PTA+K  SCN+  LS
Sbjct: 106 WILFTDIEGLVIEGD--GEINGQGSSWWEHK-----------GSRPTALKFRSCNNLRLS 165

Query: 108 NLHFINSSQAHVLLMGFRRTEIKNLVITAPESSPNTDGIHIHSSHNIIIRNAAIGTGDDC 167
            L  ++S  AH+ +       I +L I APESSPNTDGI + +S N++I++  I TGDDC
Sbjct: 166 GLTHLDSPMAHIHISECNYVTISSLRINAPESSPNTDGIDVGASSNVVIQDCIIATGDDC 225

Query: 168 ASIGDYTSHVNISHIKCGPGHGISIGSLGRGGNFVQVEDISVSNIYFKGTTNGARIKTWQ 227
            +I   TS+++IS I CGPGHGISIGSLG+ G    VE++ V N  F+GT NGARIKTWQ
Sbjct: 226 IAINSGTSNIHISGIDCGPGHGISIGSLGKDGETATVENVCVQNCNFRGTMNGARIKTWQ 285

Query: 228 -----------------------------------------QSGVHIANVSIKNLFGTSM 278
                                                     S V ++ V   N  GTS 
Sbjct: 286 GGSGYARMITFNGITLDNVENPIIIDQFYNGGDSDNAKDRKSSAVEVSKVVFSNFIGTSK 345

[Close]

BLAST of Chy5G103660 vs. ExPASy TrEMBL
Match: A0A5D3BEX0 (Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G00230 PE=3 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 1.2e-119
Identity = 237/333 (71.17%), Postives = 253/333 (75.98%), Query Frame = 0


Query: 1   MFVKRFPEEAIFNVMSY---------------------GAVGGSN------TDSS----- 60
           MFVK FPEEAIFNVMS                        VG         +DS+     
Sbjct: 18  MFVKGFPEEAIFNVMSMELWEEATQIVLNKFVRVFSYKWRVGWEQGCEEFCSDSTGKFWE 77

Query: 61  QVKSICGFAVLDGRELTRV--------------------WLAFEGISRLSITGSGIGKID 120
           +V  +  ++++   +L+++                    WLAF+GISRLSITGSGIGKID
Sbjct: 78  RVALLKSYSIVGRCKLSQINGVIVAPTSPKEWAGTDKSLWLAFKGISRLSITGSGIGKID 137

Query: 121 GRGTGWWNQSCRDHPNLESCTSIAPTAMKIISCNDTNLSNLHFINSSQAHVLLMGFRRTE 180
           GRGTGWWNQSCRDHP+LE  T IAPTA KIISCNDTNLSNLHFINSSQAHVLLM  RRTE
Sbjct: 138 GRGTGWWNQSCRDHPSLEGRTLIAPTATKIISCNDTNLSNLHFINSSQAHVLLMECRRTE 197

Query: 181 IKNLVITAPESSPNTDGIHIHSSHNIIIRNAAIGTGDDCASIGDYTSHVNISHIKCGPGH 240
           IKNLVITAPESSPNTDGIHIHSSHNIII+N  IGTGDDC S GDYTSHVNISHIKCGPGH
Sbjct: 198 IKNLVITAPESSPNTDGIHIHSSHNIIIKNTTIGTGDDCVSTGDYTSHVNISHIKCGPGH 257

Query: 241 GISIGSLGRGGNFVQVEDISVSNIYFKGTTNGARIKTWQQSGVHIANVSIKNLFGTSMSN 282
           GISIGSLG+ GNFVQVEDISVSNIYFKGTTN ARIKTWQQSGV IANVSIKNLFGTSMSN
Sbjct: 258 GISIGSLGKSGNFVQVEDISVSNIYFKGTTNRARIKTWQQSGVRIANVSIKNLFGTSMSN 317

[Close]

BLAST of Chy5G103660 vs. ExPASy TrEMBL
Match: A0A0A0LF63 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G006210 PE=3 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 8.1e-116
Identity = 219/255 (85.88%), Postives = 221/255 (86.67%), Query Frame = 0


Query: 47  VWLAFEGISRLSITGSGIGKIDGRGTGWWNQSCRDHPNLESCTSIAPTAMKIISCNDTNL 106
           +WLA EGISRL I GSGIGKIDGRGTGWWNQSCRDHPNLE CTSIAPTAMKIISCND NL
Sbjct: 40  LWLALEGISRLRINGSGIGKIDGRGTGWWNQSCRDHPNLEGCTSIAPTAMKIISCNDPNL 99

Query: 107 SNLHFINSSQAHVLLMGFRRTEIKNLVITAPESSPNTDGIHIHSSHNIIIRNAAIGTGDD 166
           SNLHFINSSQA VLLMG  RTEIKNLVITAPE SPNTDGIHIHSSHNIIIRN  IGTGDD
Sbjct: 100 SNLHFINSSQALVLLMGCIRTEIKNLVITAPERSPNTDGIHIHSSHNIIIRNTTIGTGDD 159

Query: 167 CASIGDYTSHVNISHIKCGPGHGISIGSLGRGGNFVQVEDISVSNIYFKGTTNGARIKTW 226
           C SIGDYTS +NISHIKCGPGHGISIGSLGRGGNFVQVEDISVSNIYFKGTTNGARIKTW
Sbjct: 160 CVSIGDYTSRMNISHIKCGPGHGISIGSLGRGGNFVQVEDISVSNIYFKGTTNGARIKTW 219

Query: 227 Q-------------------QSGVHIANVSIKNLFGTSMSNVAINLNCSRSVACTG-ILL 282
           Q                   QSGVHIANVSIKNLFGTSMSNVAINLNCSRSVACTG ILL
Sbjct: 220 QFSALNYSAYSAQKFFIFSKQSGVHIANVSIKNLFGTSMSNVAINLNCSRSVACTGSILL 279

[Close]

BLAST of Chy5G103660 vs. ExPASy TrEMBL
Match: A0A5A7V0D8 (Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold41G001680 PE=3 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 2.2e-113
Identity = 210/235 (89.36%), Postives = 215/235 (91.49%), Query Frame = 0


Query: 47  VWLAFEGISRLSITGSGIGKIDGRGTGWWNQSCRDHPNLESCTSIAPTAMKIISCNDTNL 106
           +WLAF+GISRLSITGSGIGKIDGRGTGWWNQSCRDHP+L         A KIISCNDTNL
Sbjct: 141 LWLAFKGISRLSITGSGIGKIDGRGTGWWNQSCRDHPSLPLL-----QATKIISCNDTNL 200

Query: 107 SNLHFINSSQAHVLLMGFRRTEIKNLVITAPESSPNTDGIHIHSSHNIIIRNAAIGTGDD 166
           SNLHFINSSQAHVLLM  RRTEIKNLVITAPESSPNTDGIHIHSSHNIII+N  IGTGDD
Sbjct: 201 SNLHFINSSQAHVLLMECRRTEIKNLVITAPESSPNTDGIHIHSSHNIIIKNTTIGTGDD 260

Query: 167 CASIGDYTSHVNISHIKCGPGHGISIGSLGRGGNFVQVEDISVSNIYFKGTTNGARIKTW 226
           C S GDYTSHVNISHIKCGPGHGISIGSLG+ GNFVQVEDISVSNIYFKGTTN ARIKTW
Sbjct: 261 CVSTGDYTSHVNISHIKCGPGHGISIGSLGKSGNFVQVEDISVSNIYFKGTTNRARIKTW 320

Query: 227 QQSGVHIANVSIKNLFGTSMSNVAINLNCSRSVACTGILLNSIQLQSVNSGQKVT 282
           QQSGV IANVSIKNLFGTSMSNVAINLNCSRSVACTGILL SIQLQSVNSGQKVT
Sbjct: 321 QQSGVRIANVSIKNLFGTSMSNVAINLNCSRSVACTGILLKSIQLQSVNSGQKVT 370

[Close]

BLAST of Chy5G103660 vs. ExPASy TrEMBL
Match: A0A5A7V0D8 (Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold41G001680 PE=3 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 9.9e-05
Identity = 28/32 (87.50%), Postives = 29/32 (90.62%), Query Frame = 0


Query: 1  MFVKRFPEEAIFNVMSYGAVGGSNTDSSQVKS 33
          MFVK FPEEAIFNV+SYGAVGGSNTDSSQ  S
Sbjct: 18 MFVKGFPEEAIFNVVSYGAVGGSNTDSSQAIS 49

HSP 2 Score: 412.9 bits (1060), Expect = 1.2e-111
Identity = 222/368 (60.33%), Postives = 247/368 (67.12%), Query Frame = 0


Query: 2   FVKRFPEEAIFNVMSYGAVGGSNTDSSQV-----KSICG--------------------- 61
           F   F +E +FNVMSYGAV G+  DSS       K+ C                      
Sbjct: 19  FTIGFSKENVFNVMSYGAVEGTTRDSSHAILKAWKAACSSKTESSTVLIPKRHTLLVKPI 78

Query: 62  -------------------FAVLDGRELTRV----WLAFEGISRLSITGSGIGKIDGRGT 121
                               A     E TR     WLAFEG+SRL++TG+GIGKIDGRGT
Sbjct: 79  VLRGPCRANSITFMIHGEIVAPTSPGEWTRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGT 138

Query: 122 GWWNQSCRDHPNLESCTSIAPTAMKIISCNDTNLSNLHFINSSQAHVLLMGFRRTEIKNL 181
            WW+QSCRDHP+L+ CTS+APTAMKIISCN+T+LSN+HFINSSQ HVL+MG +R EIKNL
Sbjct: 139 RWWSQSCRDHPDLKGCTSLAPTAMKIISCNNTSLSNIHFINSSQTHVLIMGSKRVEIKNL 198

Query: 182 VITAPESSPNTDGIHIHSSHNIIIRNAAIGTGDDCASIGDYTSHVNISHIKCGPGHGISI 241
           VITAPESSPNTDGIHIHSSHN+II+N  IGTGDDC SIGDYTS+VNISHI+CGPGHGISI
Sbjct: 199 VITAPESSPNTDGIHIHSSHNVIIKNTTIGTGDDCVSIGDYTSNVNISHIRCGPGHGISI 258

Query: 242 GSLGRGGNFVQVEDISVSNIYFKGTTNGARIKTWQ------------------------- 282
           GSLGR GNFVQVEDI VSN+YFKGTTNGARIKTWQ                         
Sbjct: 259 GSLGRSGNFVQVEDIRVSNVYFKGTTNGARIKTWQIGRGNIRRVTFQNLYFESVNNPFII 318

[Close]

BLAST of Chy5G103660 vs. ExPASy TrEMBL
Match: A0A1S4E1D2 (probable polygalacturonase At1g80170 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103497273 PE=3 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 3.1e-99
Identity = 196/279 (70.25%), Postives = 204/279 (73.12%), Query Frame = 0


Query: 1   MFVKRFPEEAIFNVMSYGAVGGSNTDSSQV-----KSIC------GFAVLDGRELTRV-- 60
           MFVK FPEEAIFNVMSYGAVGGSNTDSSQ      K+ C         ++  R+   V  
Sbjct: 18  MFVKGFPEEAIFNVMSYGAVGGSNTDSSQAISEAWKAACNSKTKSSTVLIPKRQTVMVKP 77

Query: 61  ------------------------------------WLAFEGISRLSITGSGIGKIDGRG 120
                                               WLAFEGISRLSITGS IGKIDGRG
Sbjct: 78  ILLSGPCRVKSITFMINGVIMAPTSPKEWAGTDKSLWLAFEGISRLSITGSSIGKIDGRG 137

Query: 121 TGWWNQSCRDHPNLESCTSIAPTAMKIISCNDTNLSNLHFINSSQAHVLLMGFRRTEIKN 180
           TGWWNQSCRDHP+LE  T IAPTA KIISCNDTNLSNLHFINSSQAHVLLM  RRTEIKN
Sbjct: 138 TGWWNQSCRDHPSLEGRTLIAPTATKIISCNDTNLSNLHFINSSQAHVLLMECRRTEIKN 197

Query: 181 LVITAPESSPNTDGIHIHSSHNIIIRNAAIGTGDDCASIGDYTSHVNISHIKCGPGHGIS 231
           LVITAPESSPNTDGIHIHSSHNIII+N  IGTGDDC S GDYTS VNISHIKCGPGHGIS
Sbjct: 198 LVITAPESSPNTDGIHIHSSHNIIIKNTTIGTGDDCVSTGDYTSRVNISHIKCGPGHGIS 257

[Close]

BLAST of Chy5G103660 vs. NCBI nr
Match: KAA0061603.1 (putative polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 439 bits (1128), Expect = 8.49e-151
Identity = 242/358 (67.60%), Postives = 254/358 (70.95%), Query Frame = 0


Query: 1   MFVKRFPEEAIFNVMSYGAVGGSNTDSSQ------------------------------- 60
           MFVK FPEEAIFNV+SYGAVGGSNTDSSQ                               
Sbjct: 18  MFVKGFPEEAIFNVVSYGAVGGSNTDSSQAISKAWKAACNSKTKSSTVLIPKRQTVMVKP 77

Query: 61  -------------------------VKSICGFAVLDGR-ELTRV---------------- 120
                                    +KS   + +  GR +L+++                
Sbjct: 78  ILLSGPCRAKSITFMVKKNRDFPLMIKSFLEYYLSSGRCKLSQINGVIVAPTSPKEWAGT 137

Query: 121 ----WLAFEGISRLSITGSGIGKIDGRGTGWWNQSCRDHPNLESCTSIAPTAMKIISCND 180
               WLAF+GISRLSITGSGIGKIDGRGTGWWNQSCRDHP+L         A KIISCND
Sbjct: 138 DKSLWLAFKGISRLSITGSGIGKIDGRGTGWWNQSCRDHPSLPLLQ-----ATKIISCND 197

Query: 181 TNLSNLHFINSSQAHVLLMGFRRTEIKNLVITAPESSPNTDGIHIHSSHNIIIRNAAIGT 240
           TNLSNLHFINSSQAHVLLM  RRTEIKNLVITAPESSPNTDGIHIHSSHNIII+N  IGT
Sbjct: 198 TNLSNLHFINSSQAHVLLMECRRTEIKNLVITAPESSPNTDGIHIHSSHNIIIKNTTIGT 257

Query: 241 GDDCASIGDYTSHVNISHIKCGPGHGISIGSLGRGGNFVQVEDISVSNIYFKGTTNGARI 281
           GDDC S GDYTSHVNISHIKCGPGHGISIGSLG+ GNFVQVEDISVSNIYFKGTTN ARI
Sbjct: 258 GDDCVSTGDYTSHVNISHIKCGPGHGISIGSLGKSGNFVQVEDISVSNIYFKGTTNRARI 317

[Close]

BLAST of Chy5G103660 vs. NCBI nr
Match: TYJ97813.1 (putative polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 437 bits (1124), Expect = 1.72e-150
Identity = 237/333 (71.17%), Postives = 253/333 (75.98%), Query Frame = 0


Query: 1   MFVKRFPEEAIFNVMSYGA---------------------VGGSN------TDSS----- 60
           MFVK FPEEAIFNVMS                        VG         +DS+     
Sbjct: 18  MFVKGFPEEAIFNVMSMELWEEATQIVLNKFVRVFSYKWRVGWEQGCEEFCSDSTGKFWE 77

Query: 61  QVKSICGFAVLDGRELTRV--------------------WLAFEGISRLSITGSGIGKID 120
           +V  +  ++++   +L+++                    WLAF+GISRLSITGSGIGKID
Sbjct: 78  RVALLKSYSIVGRCKLSQINGVIVAPTSPKEWAGTDKSLWLAFKGISRLSITGSGIGKID 137

Query: 121 GRGTGWWNQSCRDHPNLESCTSIAPTAMKIISCNDTNLSNLHFINSSQAHVLLMGFRRTE 180
           GRGTGWWNQSCRDHP+LE  T IAPTA KIISCNDTNLSNLHFINSSQAHVLLM  RRTE
Sbjct: 138 GRGTGWWNQSCRDHPSLEGRTLIAPTATKIISCNDTNLSNLHFINSSQAHVLLMECRRTE 197

Query: 181 IKNLVITAPESSPNTDGIHIHSSHNIIIRNAAIGTGDDCASIGDYTSHVNISHIKCGPGH 240
           IKNLVITAPESSPNTDGIHIHSSHNIII+N  IGTGDDC S GDYTSHVNISHIKCGPGH
Sbjct: 198 IKNLVITAPESSPNTDGIHIHSSHNIIIKNTTIGTGDDCVSTGDYTSHVNISHIKCGPGH 257

Query: 241 GISIGSLGRGGNFVQVEDISVSNIYFKGTTNGARIKTWQQSGVHIANVSIKNLFGTSMSN 281
           GISIGSLG+ GNFVQVEDISVSNIYFKGTTN ARIKTWQQSGV IANVSIKNLFGTSMSN
Sbjct: 258 GISIGSLGKSGNFVQVEDISVSNIYFKGTTNRARIKTWQQSGVRIANVSIKNLFGTSMSN 317

[Close]

BLAST of Chy5G103660 vs. NCBI nr
Match: XP_022143115.1 (probable polygalacturonase At1g80170 [Momordica charantia])

HSP 1 Score: 410 bits (1055), Expect = 1.78e-139
Identity = 224/368 (60.87%), Postives = 249/368 (67.66%), Query Frame = 0


Query: 2   FVKRFPEEAIFNVMSYGAVGGSNTDSSQV-----KSICGFA------------------- 61
           F   F +E +FNVMSYGAV G+  DSS       K+ C                      
Sbjct: 19  FTIGFSKENVFNVMSYGAVEGTTRDSSHAILKAWKAACSSKTESSTVLIPKRHTLLVKPI 78

Query: 62  VLDGR---------------------ELTRV----WLAFEGISRLSITGSGIGKIDGRGT 121
           VL G                      E TR     WLAFEG+SRL++TG+GIGKIDGRGT
Sbjct: 79  VLRGPCRANSITFMIHGEIVAPTSPGEWTRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGT 138

Query: 122 GWWNQSCRDHPNLESCTSIAPTAMKIISCNDTNLSNLHFINSSQAHVLLMGFRRTEIKNL 181
            WW+QSCRDHP+L+ CTS+APTAMKIISCN+T+LSN+HFINSSQ HVL+MG +R EIKNL
Sbjct: 139 RWWSQSCRDHPDLKGCTSLAPTAMKIISCNNTSLSNIHFINSSQTHVLIMGSKRVEIKNL 198

Query: 182 VITAPESSPNTDGIHIHSSHNIIIRNAAIGTGDDCASIGDYTSHVNISHIKCGPGHGISI 241
           VITAPESSPNTDGIHIHSSHN+II+N  IGTGDDC SIGDYTS+VNISHI+CGPGHGISI
Sbjct: 199 VITAPESSPNTDGIHIHSSHNVIIKNTTIGTGDDCVSIGDYTSNVNISHIRCGPGHGISI 258

Query: 242 GSLGRGGNFVQVEDISVSNIYFKGTTNGARIKTWQ------------------------- 281
           GSLGR GNFVQVEDI VSN+YFKGTTNGARIKTWQ                         
Sbjct: 259 GSLGRSGNFVQVEDIRVSNVYFKGTTNGARIKTWQIGRGNIRRVTFQNLYFESVNNPFII 318

[Close]

BLAST of Chy5G103660 vs. NCBI nr
Match: XP_016902034.1 (PREDICTED: probable polygalacturonase At1g80170 isoform X1 [Cucumis melo])

HSP 1 Score: 367 bits (943), Expect = 4.28e-124
Identity = 196/279 (70.25%), Postives = 204/279 (73.12%), Query Frame = 0


Query: 1   MFVKRFPEEAIFNVMSYGAVGGSNTDSSQV-----KSICGF------AVLDGRELTRV-- 60
           MFVK FPEEAIFNVMSYGAVGGSNTDSSQ      K+ C         ++  R+   V  
Sbjct: 18  MFVKGFPEEAIFNVMSYGAVGGSNTDSSQAISEAWKAACNSKTKSSTVLIPKRQTVMVKP 77

Query: 61  ------------------------------------WLAFEGISRLSITGSGIGKIDGRG 120
                                               WLAFEGISRLSITGS IGKIDGRG
Sbjct: 78  ILLSGPCRVKSITFMINGVIMAPTSPKEWAGTDKSLWLAFEGISRLSITGSSIGKIDGRG 137

Query: 121 TGWWNQSCRDHPNLESCTSIAPTAMKIISCNDTNLSNLHFINSSQAHVLLMGFRRTEIKN 180
           TGWWNQSCRDHP+LE  T IAPTA KIISCNDTNLSNLHFINSSQAHVLLM  RRTEIKN
Sbjct: 138 TGWWNQSCRDHPSLEGRTLIAPTATKIISCNDTNLSNLHFINSSQAHVLLMECRRTEIKN 197

Query: 181 LVITAPESSPNTDGIHIHSSHNIIIRNAAIGTGDDCASIGDYTSHVNISHIKCGPGHGIS 230
           LVITAPESSPNTDGIHIHSSHNIII+N  IGTGDDC S GDYTS VNISHIKCGPGHGIS
Sbjct: 198 LVITAPESSPNTDGIHIHSSHNIIIKNTTIGTGDDCVSTGDYTSRVNISHIKCGPGHGIS 257

[Close]

BLAST of Chy5G103660 vs. NCBI nr
Match: KAE8651530.1 (hypothetical protein Csa_019326 [Cucumis sativus])

HSP 1 Score: 365 bits (938), Expect = 9.28e-124
Identity = 194/236 (82.20%), Postives = 196/236 (83.05%), Query Frame = 0


Query: 47  VWLAFEGISRLSITGSGIGKIDGRGTGWWNQSCRDHPNLESCTSIAPTAMKIISCNDTNL 106
           +WLA EGISRL I GSGIGKIDGRGTGWWNQSCRDHPNLE CTSIAPTAMKIISCND NL
Sbjct: 40  LWLALEGISRLRINGSGIGKIDGRGTGWWNQSCRDHPNLEGCTSIAPTAMKIISCNDPNL 99

Query: 107 SNLHFINSSQAHVLLMGFRRTEIKNLVITAPESSPNTDGIHIHSSHNIIIRNAAIGTGDD 166
           SNLHFINSSQA VLLMG  RTEIKNLVITAPE SPNTDGIHIHSSHNIIIRN  IGTGD 
Sbjct: 100 SNLHFINSSQALVLLMGCIRTEIKNLVITAPERSPNTDGIHIHSSHNIIIRNTTIGTGD- 159

Query: 167 CASIGDYTSHVNISHIKCGPGHGISIGSLGRGGNFVQVEDISVSNIYFKGTTNGARIKTW 226
                 YTS +NISHIKCGPGHGISIGSLGRGGNFVQVEDIS                  
Sbjct: 160 ------YTSRMNISHIKCGPGHGISIGSLGRGGNFVQVEDIS------------------ 219

Query: 227 QQSGVHIANVSIKNLFGTSMSNVAINLNCSRSVACTG-ILLNSIQLQSVNSGQKVT 281
            QSGVHIANVSIKNLFGTSMSNVAINLNCSRSVACTG ILL SIQLQSVNSGQKVT
Sbjct: 220 -QSGVHIANVSIKNLFGTSMSNVAINLNCSRSVACTGSILLKSIQLQSVNSGQKVT 249

[Close]

BLAST of Chy5G103660 vs. TAIR 10
Match: AT1G80140.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 201.4 bits (511), Expect = 1.1e-51
Identity = 111/277 (40.07%), Postives = 150/277 (54.15%), Query Frame = 0


Query: 48  WLAFEGISRLSITGSGIGKIDGRGTGWWNQSCRDHPNLESCTSIAPTAMKIISCNDTNLS 107
           WL F+ +  L+I G G+  +DG+G  WW+  CRDHP            M   +C +  L 
Sbjct: 55  WLIFDQVDGLAIVGRGL--LDGQGKSWWDIHCRDHPG---------PMMTFSNCGNVTLK 114

Query: 108 NLHFINSSQAHVLLMGFRRTEIKNLVITAPESSPNTDGIHIHSSHNIIIRNAAIGTGDDC 167
           +L F NS+Q HVL+MG +   I ++ IT+PE+SPNTDGIHI SS  + I ++ I TGDDC
Sbjct: 115 SLRFRNSAQTHVLVMGSQNVYIDDIKITSPEASPNTDGIHITSSTAVSINHSDIATGDDC 174

Query: 168 ASIGDYTSHVNISHIKCGPGHGISIGSLGRGGNFVQVEDISVSNIYFKGTTNGARIKTW- 227
            SIGD  +++N++ + CGPGHG+SIGSLGRGG  V VE+I VS++ F GTTNGARIKTW 
Sbjct: 175 VSIGDQVNNLNVTFMNCGPGHGVSIGSLGRGGTEVTVENIRVSHVNFTGTTNGARIKTWP 234

Query: 228 -------------------------------------QQSGVHIANVSIKNLFGTSMSNV 286
                                                   GVHI  V    + GTS + V
Sbjct: 235 GGTGYVRGIEFFDIRFSSVQNPIIIDQFYGCAPTCVETMKGVHIEKVRYMKMSGTSATKV 294

[Close]

BLAST of Chy5G103660 vs. TAIR 10
Match: AT1G80170.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 185.3 bits (469), Expect = 7.9e-47
Identity = 105/269 (39.03%), Postives = 140/269 (52.04%), Query Frame = 0


Query: 46  RVWLAFEGISRLSITGSGIGKIDGRGTGWWNQSCRDHPNLESCTSIAPTAMKIISCNDTN 105
           R WL F G+SRL++ G   G ++G G  WW +SC+ H +   C   APTA+    C +  
Sbjct: 134 RKWLYFHGLSRLTVEGG--GTVNGMGQEWWERSCK-HNHSNPCRG-APTALTFHKCKNMR 193

Query: 106 LSNLHFINSSQAHVLLMGFRRTEIKNLVITAPESSPNTDGIHIHSSHNIIIRNAAIGTGD 165
           + NL+ I+S Q H+ L   RR  I  L + AP +SPNTDGIHI  S  I+I N  + TGD
Sbjct: 194 VENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNTDGIHISVSRGIVIDNTTVSTGD 253

Query: 166 DCASIGDYTSHVNISHIKCGPGHGISIGSLGRGGNFVQVEDISVSNIYFKGTTNGARIKT 225
           DC SI   ++ ++IS+I CGPGHGISIGSLG+  ++ +V DI+V       T NG RIKT
Sbjct: 254 DCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEEVRDITVDTAIISDTANGVRIKT 313

Query: 226 W---------------------------------------QQSGVHIANVSIKNLFGTSM 276
           W                                       Q S + I N+S  ++ GTS 
Sbjct: 314 WQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPCANQTSAISIENISFVHVRGTSA 373

[Close]

BLAST of Chy5G103660 vs. TAIR 10
Match: AT3G07970.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 179.1 bits (453), Expect = 5.6e-45
Identity = 95/265 (35.85%), Postives = 142/265 (53.58%), Query Frame = 0


Query: 46  RVWLAFEGISRLSITGSGIGKIDGRGTGWWNQSCRDHPNLESCTSIAPTAMKIISCNDTN 105
           R W+ FE ++ L + G   G+IDG G  WW +SC+ +P L  C   APTA+  + CN+  
Sbjct: 150 RHWIVFENVNNLRVEGG--GRIDGNGHIWWPKSCKINPQL-PCLG-APTAVTFVECNNLR 209

Query: 106 LSNLHFINSSQAHVLLMGFRRTEIKNLVITAPESSPNTDGIHIHSSHNIIIRNAAIGTGD 165
           +SN+   N+ Q H+     +  +  NL++T+P  SPNTDGIH+  + NI+I+++ + TGD
Sbjct: 210 VSNIRLENAQQMHLTFQDCKNVKALNLMVTSPADSPNTDGIHVSGTQNILIQDSIVRTGD 269

Query: 166 DCASIGDYTSHVNISHIKCGPGHGISIGSLGRGGNFVQVEDISVSNIYFKGTTNGARIKT 225
           DC SI   + +V  + I CGPGHGISIGSLG   +   V ++ V+     GTTNG RIKT
Sbjct: 270 DCISIVSGSENVRATGITCGPGHGISIGSLGEDNSEAYVSNVVVNKATLIGTTNGVRIKT 329

Query: 226 W---------------------------------------QQSGVHIANVSIKNLFGTSM 272
           W                                       Q+S V ++NV  KN+ GTS 
Sbjct: 330 WQGGHGMAKNIIFQDIIMKNVTNPIIINQDYCDRVEACPEQKSAVQVSNVLYKNIQGTSS 389

[Close]

BLAST of Chy5G103660 vs. TAIR 10
Match: AT3G57510.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 178.7 bits (452), Expect = 7.4e-45
Identity = 102/274 (37.23%), Postives = 142/274 (51.82%), Query Frame = 0


Query: 48  WLAFEGISRLSITGSGIGKIDGRGTGWWNQSCRDHPNLESCTSIAPTAMKIISCNDTNLS 107
           WL  E ++ LSI G   G I+G G  WW  SC+   + + CT  APTA+ + +  + N+ 
Sbjct: 149 WLILEDVNNLSIDGGSTGIINGNGKTWWQNSCKIDKS-KPCTK-APTALTLYNLKNLNVK 208

Query: 108 NLHFINSSQAHVLLMGFRRTEIKNLVITAPESSPNTDGIHIHSSHNIIIRNAAIGTGDDC 167
           NL   N+ Q  + +    + E+ N+ ITAP  SPNTDGIHI ++ NI + N+ IGTGDDC
Sbjct: 209 NLRVKNAQQIQISIEKCNKVEVSNVEITAPGDSPNTDGIHITNTQNIRVSNSDIGTGDDC 268

Query: 168 ASIGDYTSHVNISHIKCGPGHGISIGSLGRGGNFVQVEDISVSNIYFKGTTNGARIKTW- 227
            SI D T ++ I  + CGPGHGISIGSLG   +   V  I+V    F  + NG RIKT+ 
Sbjct: 269 ISIEDGTQNLQIFDLTCGPGHGISIGSLGDDNSKAYVSGINVDGAKFSESDNGVRIKTYQ 328

Query: 228 -------------------------------------QQSGVHIANVSIKNLFGTSMSNV 284
                                                Q+S V + NV  KN+ GTS ++V
Sbjct: 329 GGSGTAKNIKFQNIRMENVKNPIIIDQDYCDKDKCEDQESAVQVKNVVYKNISGTSATDV 388

[Close]

BLAST of Chy5G103660 vs. TAIR 10
Match: AT3G15720.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 177.9 bits (450), Expect = 1.3e-44
Identity = 101/271 (37.27%), Postives = 137/271 (50.55%), Query Frame = 0


Query: 48  WLAFEGISRLSITGSGIGKIDGRGTGWWNQSCRDHPNLESCTSIAPTAMKIISCNDTNLS 107
           W+ F  I  L I G   G+I+G+G+ WW                 PTA+K  SCN+  LS
Sbjct: 106 WILFTDIEGLVIEGD--GEINGQGSSWWEHK-----------GSRPTALKFRSCNNLRLS 165

Query: 108 NLHFINSSQAHVLLMGFRRTEIKNLVITAPESSPNTDGIHIHSSHNIIIRNAAIGTGDDC 167
            L  ++S  AH+ +       I +L I APESSPNTDGI + +S N++I++  I TGDDC
Sbjct: 166 GLTHLDSPMAHIHISECNYVTISSLRINAPESSPNTDGIDVGASSNVVIQDCIIATGDDC 225

Query: 168 ASIGDYTSHVNISHIKCGPGHGISIGSLGRGGNFVQVEDISVSNIYFKGTTNGARIKTWQ 227
            +I   TS+++IS I CGPGHGISIGSLG+ G    VE++ V N  F+GT NGARIKTWQ
Sbjct: 226 IAINSGTSNIHISGIDCGPGHGISIGSLGKDGETATVENVCVQNCNFRGTMNGARIKTWQ 285

Query: 228 -----------------------------------------QSGVHIANVSIKNLFGTSM 278
                                                     S V ++ V   N  GTS 
Sbjct: 286 GGSGYARMITFNGITLDNVENPIIIDQFYNGGDSDNAKDRKSSAVEVSKVVFSNFIGTSK 345

The following BLAST results are available for this feature:

BLAST of Chy5G103660 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94AJ5	1.1e-45	39.03	Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017...	[more]
P48978	1.4e-45	39.10	Polygalacturonase OS=Malus domestica OX=3750 PE=2 SV=1	[more]
Q9SFB7	7.9e-44	35.85	Polygalacturonase QRT2 OS=Arabidopsis thaliana OX=3702 GN=QRT2 PE=1 SV=2	[more]
O23147	1.0e-43	37.23	Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1	[more]
Q9LW07	1.8e-43	37.27	Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g1572...	[more]

BLAST of Chy5G103660 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BEX0	1.2e-119	71.17	Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A0A0LF63	8.1e-116	85.88	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G006210 PE=3 SV=1	[more]
A0A5A7V0D8	2.2e-113	89.36	Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff...	[more]
A0A5A7V0D8	9.9e-05	87.50	Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff...	[more]
A0A1S4E1D2	3.1e-99	70.25	probable polygalacturonase At1g80170 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10...	[more]

BLAST of Chy5G103660 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0061603.1	8.49e-151	67.60	putative polygalacturonase [Cucumis melo var. makuwa]	[more]
TYJ97813.1	1.72e-150	71.17	putative polygalacturonase [Cucumis melo var. makuwa]	[more]
XP_022143115.1	1.78e-139	60.87	probable polygalacturonase At1g80170 [Momordica charantia]	[more]
XP_016902034.1	4.28e-124	70.25	PREDICTED: probable polygalacturonase At1g80170 isoform X1 [Cucumis melo]	[more]
KAE8651530.1	9.28e-124	82.20	hypothetical protein Csa_019326 [Cucumis sativus]	[more]

BLAST of Chy5G103660 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G80140.1	1.1e-51	40.07	Pectin lyase-like superfamily protein	[more]
AT1G80170.1	7.9e-47	39.03	Pectin lyase-like superfamily protein	[more]
AT3G07970.1	5.6e-45	35.85	Pectin lyase-like superfamily protein	[more]
AT3G57510.1	7.4e-45	37.23	Pectin lyase-like superfamily protein	[more]
AT3G15720.1	1.3e-44	37.27	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 36..298 e-value: 4.5E-63 score: 215.6
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 44..236 e-value: 1.3E-50 score: 172.4
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 181..194
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 46..228 coord: 228..275
None	No IPR available	PANTHER	PTHR31375:SF229	POLYGALACTURONASE-LIKE	coord: 228..275
None	No IPR available	PANTHER	PTHR31375:SF229	POLYGALACTURONASE-LIKE	coord: 46..228
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 7..282

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy5G103660.1	Chy5G103660.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity