Chy5G103350 (gene) Cucumber (hystrix) v1

BLAST of Chy5G103350 vs. ExPASy Swiss-Prot
Match: Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 4.9e-22
Identity = 75/227 (33.04%), Postives = 107/227 (47.14%), Query Frame = 0

Query: 1   MPLYLSELFQLKTLSAFVVGLKKGCTTTELGSLRKLKGLLVILCLEHVGSKEEAKSAKLV 60
           MP     L  L+TL+ F V    G   +ELG L  L G L I+ L+ V    +A  A L 
Sbjct: 664 MPRRFGRLKSLQTLTTFFVSASDGSRISELGGLHDLHGKLKIVELQRVVDVADAAEANLN 723

Query: 61  EKEKVEELHFVWSTESDRIVKN------ENDFEVLEGLEPHNNLKHLEIESFSGGQFPNQ 120
            K+ + E+ FVW T S     N      +N+ EV E L PH +++ L IE + G +FP+ 
Sbjct: 724 SKKHLREIDFVWRTGSSSSENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDW 783

Query: 121 I---FVENLVKITLIECGNCEKLPMLGQLTNLEILVIFRLRKVESIGNEFYGNQR----G 180
           +       +V I L EC  C  LP LGQL  L+ L I  +  ++SIG +FY + +     
Sbjct: 784 LSDPSFSRIVCIRLRECQYCTSLPSLGQLPCLKELHISGMVGLQSIGRKFYFSDQQLRDQ 843

Query: 181 SSSVFPKLKEFYVDEMDSLVEWEEAVSNNVKAFPRLECLHIISCKKL 215
               F  L+    D +    EW +        FP L+ L I+ C +L
Sbjct: 844 DQQPFRSLETLRFDNLPDWQEWLDVRVTRGDLFPSLKKLFILRCPEL 890

BLAST of Chy5G103350 vs. ExPASy Swiss-Prot
Match: Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)

HSP 1 Score: 87.4 bits (215), Expect = 3.1e-16
Identity = 70/209 (33.49%), Postives = 101/209 (48.33%), Query Frame = 0

Query: 11  LKTLSAFVVGLKKGCTTTELGSLRKLKGLLVILCLEHVGSKEEAKSAKLVEKEKVEELHF 70
           LK+LS FV+G +KG    EL +L  L G + I  L+ V    +AK A L  K  +  L  
Sbjct: 621 LKSLSCFVIGKRKGHQLGELKNL-NLYGSISITKLDRVKKDTDAKEANLSAKANLHSLCL 680

Query: 71  VWSTESDRIVKNENDFEVLEGLEPHNNLKHLEIESFSGGQFP---NQIFVENLVKITLIE 130
            W  +     K+  D EVLE L+PH+NLK+LEI  F G + P   NQ  ++N+V I +  
Sbjct: 681 SWDLDG----KHRYDSEVLEALKPHSNLKYLEINGFGGIRLPDWMNQSVLKNVVSIRIRG 740

Query: 131 CGNCEKLPMLGQLTNLEILVIFR-LRKVESIGNEFYGNQRGSSSVFPKLKEFYVDEMDSL 190
           C NC  LP  G+L  LE L +      VE + +  +  +      FP L++  + +  +L
Sbjct: 741 CENCSCLPPFGELPCLESLELHTGSADVEYVEDNVHPGR------FPSLRKLVIWDFSNL 800

Query: 191 VEWEEAVSNNVKAFPRLECLHIISCKKLV 216
               +      K FP LE +    C   V
Sbjct: 801 KGLLK--MEGEKQFPVLEEMTFYWCPMFV 816

BLAST of Chy5G103350 vs. ExPASy Swiss-Prot
Match: Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)

HSP 1 Score: 87.0 bits (214), Expect = 4.0e-16
Identity = 78/223 (34.98%), Postives = 107/223 (47.98%), Query Frame = 0

Query: 1   MPLYLSELFQLKTLSAFVVGLKKGCTTTELGSLRKLKGLLVILCLEHVGSKEEAKSAKLV 60
           MP  +  L  LKTL  FVVG +KG    EL +L  L+G + I  LE V +  EAK A L 
Sbjct: 620 MPPRIGLLTCLKTLGYFVVGERKGYQLGELRNL-NLRGAISITHLERVKNDMEAKEANLS 679

Query: 61  EKEKVEELHFVWSTESDRIVKNEN-DFEVLEGLEPHNNLKHLEIESFSGGQFP---NQIF 120
            K  +  L   W    DR  + E+ + +VLE L+PH NLK+LEI  F G   P   N   
Sbjct: 680 AKANLHSLSMSW----DRPNRYESEEVKVLEALKPHPNLKYLEIIDFCGFCLPDWMNHSV 739

Query: 121 VENLVKITLIECGNCEKLPMLGQLTNLEILVI----FRLRKVESIGNEFYGNQRGSSSVF 180
           ++N+V I +  C NC  LP  G+L  LE L +      +  VE  G  F   +R     F
Sbjct: 740 LKNVVSILISGCENCSCLPPFGELPCLESLELQDGSVEVEYVEDSG--FLTRRR-----F 799

Query: 181 PKLKEFYVDEMDSLVEWEEAVSNNVKAFPRLECLHIISCKKLV 216
           P L++ ++    +L   +       + FP LE + I  C   V
Sbjct: 800 PSLRKLHIGGFCNLKGLQR--MKGAEQFPVLEEMKISDCPMFV 828

BLAST of Chy5G103350 vs. ExPASy Swiss-Prot
Match: Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 4.5e-15
Identity = 68/209 (32.54%), Postives = 105/209 (50.24%), Query Frame = 0

Query: 11  LKTLSAFVVGLKKGCTTTELGSLRKLKGLLVILCLEHVGSKEEAKSAKLVEKEKVEELHF 70
           LKTL  F+VG KKG    EL +L  L G + I  LE V +  +A+ A L  K  ++ L  
Sbjct: 622 LKTLGFFIVGSKKGYQLGELKNL-NLCGSISITHLERVKNDTDAE-ANLSAKANLQSLSM 681

Query: 71  VWSTESDRIVKNENDFEVLEGLEPHNNLKHLEIESFSGGQFP---NQIFVENLVKITLIE 130
            W  +     +++ + +VLE L+PH NLK+LEI +F G +FP   N   +E ++ + +  
Sbjct: 682 SWDNDGPNRYESK-EVKVLEALKPHPNLKYLEIIAFGGFRFPSWINHSVLEKVISVRIKS 741

Query: 131 CGNCEKLPMLGQLTNLEILVIFR-LRKVESIGNEFYGNQRGSSSVFPKLKEFYVDEMDSL 190
           C NC  LP  G+L  LE L +     +VE +  +   ++  +   FP LK+  +    SL
Sbjct: 742 CKNCLCLPPFGELPCLENLELQNGSAEVEYVEEDDVHSRFSTRRSFPSLKKLRIWFFRSL 801

Query: 191 VEWEEAVSNNVKAFPRLECLHIISCKKLV 216
               +      + FP LE + I+ C   V
Sbjct: 802 KGLMK--EEGEEKFPMLEEMAILYCPLFV 825

BLAST of Chy5G103350 vs. ExPASy TrEMBL
Match: A0A0A0LJ04 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G008000 PE=4 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 2.9e-126
Identity = 244/308 (79.22%), Postives = 250/308 (81.17%), Query Frame = 0

Query: 1   MPLYLSELFQLKTLSAFVVGLKKGCTTTELGSLRKLKGLLVILCLEHVGSKEEAKSAKLV 60
           MP YLSEL QL+TL AF VG + G   +ELG LR LKGLL +  LEHV SKEEAK+AKLV
Sbjct: 550 MPPYLSELVQLQTLFAFAVGFETGRKISELGGLRNLKGLLKLHRLEHVESKEEAKAAKLV 609

Query: 61  EKEKVEELHFVWSTESDRIVKNENDFEVLEGLEPHNNLKHLEIESFSGGQFPNQIFVENL 120
           EKEKVEELHFVWS                                   GQFPNQIFVENL
Sbjct: 610 EKEKVEELHFVWS-----------------------------------GQFPNQIFVENL 669

Query: 121 VKITLIECGNCEKLPMLGQLTN-LEILVIFRLRKVESIGNEFYGNQRGSSS-VFPKLKEF 180
           VKITLIECGNCEKLPMLGQLT  LEILVIFRLRKVESIGNEFYGNQR SSS VFPKLKEF
Sbjct: 670 VKITLIECGNCEKLPMLGQLTKYLEILVIFRLRKVESIGNEFYGNQRRSSSTVFPKLKEF 729

Query: 181 YVDEMDSLVEWEEAVSN-NVKAFPRLECLHIISCKKLVKIPDVFGYCDEDDGEKHLKFVE 240
           YVDEMDSLVEWEEAVSN NVKAFPRLECLHIISCKKL+KIPDVFGYCDEDDGEKHLKFVE
Sbjct: 730 YVDEMDSLVEWEEAVSNYNVKAFPRLECLHIISCKKLLKIPDVFGYCDEDDGEKHLKFVE 789

Query: 241 IRFCDQLKELTNGIQSCHSIQHLVLDKGSNLWSFVTTTTRGGPLAHLLFQRPINFFERRH 300
            RFCDQLKELTNGIQSCHSIQH VLDKGSNLWSFVTTTTRGGPLAHLLFQRPI FFERRH
Sbjct: 790 FRFCDQLKELTNGIQSCHSIQHFVLDKGSNLWSFVTTTTRGGPLAHLLFQRPIIFFERRH 822

Query: 301 GSFMNKRI 306
           GSFMNKRI
Sbjct: 850 GSFMNKRI 822

BLAST of Chy5G103350 vs. ExPASy TrEMBL
Match: A0A0A0LNG8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G433340 PE=4 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 7.3e-85
Identity = 180/297 (60.61%), Postives = 213/297 (71.72%), Query Frame = 0

Query: 1   MPLYLSELFQLKTLSAFVVGLKKGCTTTELGSLRKLKGLLVILCLEHVGSKEEAKSAKLV 60
           M  YLS+L QL+TL  F VG +KGC  TELG LR LKGLL +  LEHV SKEEA++AKLV
Sbjct: 642 MSPYLSQLIQLETLPKFAVGFEKGCKITELGVLRNLKGLLKLQRLEHVESKEEAETAKLV 701

Query: 61  EKEKVEELHFVWSTESDRIVKNENDFEVLEGLEPHNNLKHLEIESFSGGQFPNQIFVENL 120
           EKE +EE+HFVW+ E  R V+N+ND EVLEGL+P  N+++L I+ F GG  PNQ FVENL
Sbjct: 702 EKENLEEVHFVWTKERKRKVENKNDLEVLEGLQPPKNVEYLRIKYFLGGCLPNQTFVENL 761

Query: 121 VKITLIECGNCEKLPMLGQLTNLEILVIFRLRKVESIGNEFYGNQRGSS-SVFPKLKEFY 180
           VKI L +CGNCEKLP LGQL NLEIL I    +V+SIGNEFYGN   +  S+FP+LKE Y
Sbjct: 762 VKIELRDCGNCEKLPRLGQLGNLEILDISWFERVKSIGNEFYGNSSNNQRSLFPRLKELY 821

Query: 181 VDEMDSLVEWEEAVSNNVKAFPRLECLHIISCKKLVKIPDVFGYCDEDDGEKHLKFVEIR 240
           VDEM  + EWEE V +NVKAFPRLE L+I  C+ LVKIPDVFGYCDE  GEKHL+ VEI 
Sbjct: 822 VDEMRRIGEWEE-VGSNVKAFPRLERLYIGCCRDLVKIPDVFGYCDE-YGEKHLEVVEI- 881

Query: 241 FCDQLKELTNGIQSCHSIQHLVLDKGSNLWSFVTTTTRGGPLAHLLFQRPINFFERR 297
                            I+HL LD+ SNLWSFV  TT+GG LA+LL +R  +FF  R
Sbjct: 882 -----------------IEHLWLDRPSNLWSFV--TTQGGALANLLSRRTRSFFYYR 916

BLAST of Chy5G103350 vs. ExPASy TrEMBL
Match: A0A6J1D340 (disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111017143 PE=4 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 2.1e-68
Identity = 142/270 (52.59%), Postives = 185/270 (68.52%), Query Frame = 0

Query: 1   MPLYLSELFQLKTLSAFVVGLKKGCTTTELGSLRKLKGLLVILCLEHVGSKEEAKSAKLV 60
           MP Y  +L QL+TLS FVVG + G   +ELG L+ LKG L +  LE V SKEEA++AKLV
Sbjct: 650 MPAYFGKLTQLQTLSYFVVGFENGHKISELGLLKNLKGSLNLCFLEKVESKEEARAAKLV 709

Query: 61  EKEKVEELHFVWSTESDRIVKNE-NDFEVLEGLEPHNNLKHLEIESFSGGQFPNQIFVEN 120
           EKE ++EL F WS    R   +   DFE+LEGL+PH NL+ L IE  +    PN IFVEN
Sbjct: 710 EKENLDELEFEWSQSHLRKDNDSYKDFEILEGLQPHKNLRSLSIERSAIKSLPNNIFVEN 769

Query: 121 LVKITLIECGNCEKLPMLGQLTNLEILVIFRLRKVESIGNEFYGNQRGSSSVFPKLKEFY 180
           L  I+L +C NCE+LPMLGQL NLE L ++ L  V+SI NEFYGN     ++FPKL+ F 
Sbjct: 770 LAVISLRDCQNCERLPMLGQLRNLERLEVYGLHSVQSISNEFYGNDCNQRTLFPKLRAFL 829

Query: 181 VDEMDSLVEWEE-AVSNNVKAFPRLECLHIISCKKLVKIPDVFGYCDEDDGEKHLKFVEI 240
           + +M++L +W+E  V++N+  FP LE L+I  C KL+ IPDVFGYCDE +G +HL+ V I
Sbjct: 830 LSDMENLEQWKEVTVASNLTTFPCLESLNIWRCPKLMNIPDVFGYCDE-NGVEHLRHVTI 889

Query: 241 RFCDQLKELTNGIQSCHSIQHLVLDKGSNL 269
             CD L +L NG+Q CHSI+ L ++  SNL
Sbjct: 890 SGCDGLNKLPNGLQFCHSIEDLTIEDCSNL 918

BLAST of Chy5G103350 vs. ExPASy TrEMBL
Match: A0A6J1CUE1 (putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC111014667 PE=4 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 6.0e-63
Identity = 135/270 (50.00%), Postives = 175/270 (64.81%), Query Frame = 0

Query: 1   MPLYLSELFQLKTLSAFVVGLKKGCTTTELGSLRKLKGLLVILCLEHVGSKEEAKSAKLV 60
           MP +L +L QL+ LS+FVVG  + C   ELG  + LKG L + CLE V SK+EA +A LV
Sbjct: 454 MPQHLRQLIQLQMLSSFVVGFDERCKIEELGPFKNLKGGLNLFCLERVRSKKEAMAANLV 513

Query: 61  EKEKVEELHFVWSTESDRIVKNENDFEVLEGLEPHNNLKHLEIESFSGGQFPNQIFVENL 120
           EKE +  L F WS   +R   N NDF+VLEGL+PH NL+ L I +FSG   PN IFVENL
Sbjct: 514 EKENISYLRFQWSLIGEREGSNYNDFDVLEGLQPHKNLQVLRIRNFSGELLPNGIFVENL 573

Query: 121 VKITLIECGNCEKLPMLGQLTNLEILVIFRLRKVESIGNEFYGNQRGSSSVFPKLKEFYV 180
           V+I L +C  CE LPMLGQL+ LE+L I  L  V SIG EFYGN R + ++FPKLK F++
Sbjct: 574 VEIYLHDCERCETLPMLGQLSKLEVLEIHCLHSVRSIGEEFYGNYRNARTLFPKLKTFHI 633

Query: 181 DEMDSLVEWEEAVS-NNVKAFPRLECLHIISCKKLVKIPDVFGYCDEDD-GEKHLKFVEI 240
            EM SL  WEE  S +N   FP LE L I+ C +L  IP+ F   D++  G   L+ ++I
Sbjct: 634 SEMSSLEHWEEIKSVSNGTIFPHLESLSIVCCLRLKSIPNFFASHDKERLGSAKLRSLKI 693

Query: 241 RFCDQLKELTNGIQSCHSIQHLVLDKGSNL 269
             C+ L EL NG++ C S++++ L   SNL
Sbjct: 694 LGCENLIELPNGLEFCSSLKNMWLSNCSNL 723

BLAST of Chy5G103350 vs. ExPASy TrEMBL
Match: A0A6J1FWC6 (disease resistance protein RGA2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448744 PE=4 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 1.3e-62
Identity = 132/265 (49.81%), Postives = 177/265 (66.79%), Query Frame = 0

Query: 1   MPLYLSELFQLKTLSAFVVGLKKGCTTTELGSLRKLKGLLVILCLEHVGSKEEAKSAKLV 60
           MP +LS+L  L+TLS F+VG +K C   ELG L+ LKG L I  LE V S+EEAKSA  +
Sbjct: 626 MPPHLSQLIHLQTLSCFMVGFEKSCEIAELGPLKNLKGSLKISNLERVKSREEAKSANFM 685

Query: 61  EKEKVEE--LHFVWSTESDRIVKNENDFEVLEGLEPHNNLKHLEIESFSGGQFPNQIFVE 120
           EKEK+ E  L + WS E++    N N ++VLEGL+PH  L  L I SF G    N IFV+
Sbjct: 686 EKEKLRELVLEWGWSNETEDHSSNHNGYQVLEGLQPHKKLHSLSIHSFVGECLANNIFVD 745

Query: 121 NLVKITLIECGNCEKLPMLGQLTNLEILVIFRLRKVESIGNEFYGNQRGSSSVFPKLKEF 180
           NLV I+L++C NC +LPMLG L +LE L++  L  V SIG+EFYGN     ++FPK+K F
Sbjct: 746 NLVAISLVDCRNCGRLPMLGHLNSLEELLVSGLNGVRSIGSEFYGNHSNQRALFPKVKIF 805

Query: 181 YVDEMDSLVEWEE-AVSNNVKAFPRLECLHIISCKKLVKIPDVFGYCDEDDGEKHLKFVE 240
            + E++ L +WEE  V  +V  FP+L+ L I  C +L+ I D+FG CDE +G +HL+ ++
Sbjct: 806 KLIEVERLEQWEEVTVGAHVTTFPQLQVLEISKCPRLLSISDLFGCCDE-NGVQHLRVLQ 865

Query: 241 IRFCDQLKELTNGIQSCHSIQHLVL 263
           I  C +L +L NG+Q CHSIQHL L
Sbjct: 866 ILECSKLTKLPNGLQFCHSIQHLRL 889

BLAST of Chy5G103350 vs. NCBI nr
Match: KAE8651533.1 (hypothetical protein Csa_019479 [Cucumis sativus])

HSP 1 Score: 325 bits (834), Expect = 1.65e-109
Identity = 164/173 (94.80%), Postives = 165/173 (95.38%), Query Frame = 0

Query: 136 MLGQLTN-LEILVIFRLRKVESIGNEFYGNQRGSSS-VFPKLKEFYVDEMDSLVEWEEAV 195
           MLGQLT  LEILVIFRLRKVESIGNEFYGNQR SSS VFPKLKEFYVDEMDSLVEWEEAV
Sbjct: 1   MLGQLTKYLEILVIFRLRKVESIGNEFYGNQRRSSSTVFPKLKEFYVDEMDSLVEWEEAV 60

Query: 196 SN-NVKAFPRLECLHIISCKKLVKIPDVFGYCDEDDGEKHLKFVEIRFCDQLKELTNGIQ 255
           SN NVKAFPRLECLHIISCKKL+KIPDVFGYCDEDDGEKHLKFVE RFCDQLKELTNGIQ
Sbjct: 61  SNYNVKAFPRLECLHIISCKKLLKIPDVFGYCDEDDGEKHLKFVEFRFCDQLKELTNGIQ 120

Query: 256 SCHSIQHLVLDKGSNLWSFVTTTTRGGPLAHLLFQRPINFFERRHGSFMNKRI 305
           SCHSIQH VLDKGSNLWSFVTTTTRGGPLAHLLFQRPI FFERRHGSFMNKRI
Sbjct: 121 SCHSIQHFVLDKGSNLWSFVTTTTRGGPLAHLLFQRPIIFFERRHGSFMNKRI 173

BLAST of Chy5G103350 vs. NCBI nr
Match: XP_011650061.1 (putative disease resistance protein RGA3 [Cucumis sativus] >KGN63368.1 hypothetical protein Csa_022194 [Cucumis sativus])

HSP 1 Score: 318 bits (814), Expect = 3.56e-97
Identity = 178/291 (61.17%), Postives = 211/291 (72.51%), Query Frame = 0

Query: 4   YLSELFQLKTLSAFVVGLKKGCTTTELGSLRKLKGLLVILCLEHVGSKEEAKSAKLVEKE 63
           YLS+L QL+TL  F VG +KGC  TELG LR LKGLL +  LEHV SKEEA++AKLVEKE
Sbjct: 645 YLSQLIQLETLPKFAVGFEKGCKITELGVLRNLKGLLKLQRLEHVESKEEAETAKLVEKE 704

Query: 64  KVEELHFVWSTESDRIVKNENDFEVLEGLEPHNNLKHLEIESFSGGQFPNQIFVENLVKI 123
            +EE+HFVW+ E  R V+N+ND EVLEGL+P  N+++L I+ F GG  PNQ FVENLVKI
Sbjct: 705 NLEEVHFVWTKERKRKVENKNDLEVLEGLQPPKNVEYLRIKYFLGGCLPNQTFVENLVKI 764

Query: 124 TLIECGNCEKLPMLGQLTNLEILVIFRLRKVESIGNEFYGNQRGSS-SVFPKLKEFYVDE 183
            L +CGNCEKLP LGQL NLEIL I    +V+SIGNEFYGN   +  S+FP+LKE YVDE
Sbjct: 765 ELRDCGNCEKLPRLGQLGNLEILDISWFERVKSIGNEFYGNSSNNQRSLFPRLKELYVDE 824

Query: 184 MDSLVEWEEAVSNNVKAFPRLECLHIISCKKLVKIPDVFGYCDEDDGEKHLKFVEIRFCD 243
           M  + EWEE V +NVKAFPRLE L+I  C+ LVKIPDVFGYCDE  GEKHL+ VEI    
Sbjct: 825 MRRIGEWEE-VGSNVKAFPRLERLYIGCCRDLVKIPDVFGYCDEY-GEKHLEVVEI---- 884

Query: 244 QLKELTNGIQSCHSIQHLVLDKGSNLWSFVTTTTRGGPLAHLLFQRPINFF 293
                         I+HL LD+ SNLWSFVTT  +GG LA+LL +R  +FF
Sbjct: 885 --------------IEHLWLDRPSNLWSFVTT--QGGALANLLSRRTRSFF 913

BLAST of Chy5G103350 vs. NCBI nr
Match: XP_022148515.1 (disease resistance protein RGA2-like [Momordica charantia])

HSP 1 Score: 263 bits (672), Expect = 3.57e-76
Identity = 142/270 (52.59%), Postives = 185/270 (68.52%), Query Frame = 0

Query: 1   MPLYLSELFQLKTLSAFVVGLKKGCTTTELGSLRKLKGLLVILCLEHVGSKEEAKSAKLV 60
           MP Y  +L QL+TLS FVVG + G   +ELG L+ LKG L +  LE V SKEEA++AKLV
Sbjct: 650 MPAYFGKLTQLQTLSYFVVGFENGHKISELGLLKNLKGSLNLCFLEKVESKEEARAAKLV 709

Query: 61  EKEKVEELHFVWSTESDRIVKNE-NDFEVLEGLEPHNNLKHLEIESFSGGQFPNQIFVEN 120
           EKE ++EL F WS    R   +   DFE+LEGL+PH NL+ L IE  +    PN IFVEN
Sbjct: 710 EKENLDELEFEWSQSHLRKDNDSYKDFEILEGLQPHKNLRSLSIERSAIKSLPNNIFVEN 769

Query: 121 LVKITLIECGNCEKLPMLGQLTNLEILVIFRLRKVESIGNEFYGNQRGSSSVFPKLKEFY 180
           L  I+L +C NCE+LPMLGQL NLE L ++ L  V+SI NEFYGN     ++FPKL+ F 
Sbjct: 770 LAVISLRDCQNCERLPMLGQLRNLERLEVYGLHSVQSISNEFYGNDCNQRTLFPKLRAFL 829

Query: 181 VDEMDSLVEWEEA-VSNNVKAFPRLECLHIISCKKLVKIPDVFGYCDEDDGEKHLKFVEI 240
           + +M++L +W+E  V++N+  FP LE L+I  C KL+ IPDVFGYCDE+ G +HL+ V I
Sbjct: 830 LSDMENLEQWKEVTVASNLTTFPCLESLNIWRCPKLMNIPDVFGYCDEN-GVEHLRHVTI 889

Query: 241 RFCDQLKELTNGIQSCHSIQHLVLDKGSNL 268
             CD L +L NG+Q CHSI+ L ++  SNL
Sbjct: 890 SGCDGLNKLPNGLQFCHSIEDLTIEDCSNL 918

BLAST of Chy5G103350 vs. NCBI nr
Match: XP_022145154.1 (putative disease resistance protein RGA3 [Momordica charantia])

HSP 1 Score: 245 bits (625), Expect = 1.37e-70
Identity = 135/270 (50.00%), Postives = 175/270 (64.81%), Query Frame = 0

Query: 1   MPLYLSELFQLKTLSAFVVGLKKGCTTTELGSLRKLKGLLVILCLEHVGSKEEAKSAKLV 60
           MP +L +L QL+ LS+FVVG  + C   ELG  + LKG L + CLE V SK+EA +A LV
Sbjct: 454 MPQHLRQLIQLQMLSSFVVGFDERCKIEELGPFKNLKGGLNLFCLERVRSKKEAMAANLV 513

Query: 61  EKEKVEELHFVWSTESDRIVKNENDFEVLEGLEPHNNLKHLEIESFSGGQFPNQIFVENL 120
           EKE +  L F WS   +R   N NDF+VLEGL+PH NL+ L I +FSG   PN IFVENL
Sbjct: 514 EKENISYLRFQWSLIGEREGSNYNDFDVLEGLQPHKNLQVLRIRNFSGELLPNGIFVENL 573

Query: 121 VKITLIECGNCEKLPMLGQLTNLEILVIFRLRKVESIGNEFYGNQRGSSSVFPKLKEFYV 180
           V+I L +C  CE LPMLGQL+ LE+L I  L  V SIG EFYGN R + ++FPKLK F++
Sbjct: 574 VEIYLHDCERCETLPMLGQLSKLEVLEIHCLHSVRSIGEEFYGNYRNARTLFPKLKTFHI 633

Query: 181 DEMDSLVEWEEAVS-NNVKAFPRLECLHIISCKKLVKIPDVFGYCDEDD-GEKHLKFVEI 240
            EM SL  WEE  S +N   FP LE L I+ C +L  IP+ F   D++  G   L+ ++I
Sbjct: 634 SEMSSLEHWEEIKSVSNGTIFPHLESLSIVCCLRLKSIPNFFASHDKERLGSAKLRSLKI 693

Query: 241 RFCDQLKELTNGIQSCHSIQHLVLDKGSNL 268
             C+ L EL NG++ C S++++ L   SNL
Sbjct: 694 LGCENLIELPNGLEFCSSLKNMWLSNCSNL 723

BLAST of Chy5G103350 vs. NCBI nr
Match: XP_022944234.1 (disease resistance protein RGA2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 244 bits (624), Expect = 1.12e-69
Identity = 143/316 (45.25%), Postives = 193/316 (61.08%), Query Frame = 0

Query: 1   MPLYLSELFQLKTLSAFVVGLKKGCTTTELGSLRKLKGLLVILCLEHVGSKEEAKSAKLV 60
           MP +LS+L  L+TLS F+VG +K C   ELG L+ LKG L I  LE V S+EEAKSA  +
Sbjct: 626 MPPHLSQLIHLQTLSCFMVGFEKSCEIAELGPLKNLKGSLKISNLERVKSREEAKSANFM 685

Query: 61  EKEKVEEL--HFVWSTESDRIVKNENDFEVLEGLEPHNNLKHLEIESFSGGQFPNQIFVE 120
           EKEK+ EL   + WS E++    N N ++VLEGL+PH  L  L I SF G    N IFV+
Sbjct: 686 EKEKLRELVLEWGWSNETEDHSSNHNGYQVLEGLQPHKKLHSLSIHSFVGECLANNIFVD 745

Query: 121 NLVKITLIECGNCEKLPMLGQLTNLEILVIFRLRKVESIGNEFYGNQRGSSSVFPKLKEF 180
           NLV I+L++C NC +LPMLG L +LE L++  L  V SIG+EFYGN     ++FPK+K F
Sbjct: 746 NLVAISLVDCRNCGRLPMLGHLNSLEELLVSGLNGVRSIGSEFYGNHSNQRALFPKVKIF 805

Query: 181 YVDEMDSLVEWEEA-VSNNVKAFPRLECLHIISCKKLVKIPDVFGYCDEDDGEKHLKFVE 240
            + E++ L +WEE  V  +V  FP+L+ L I  C +L+ I D+FG CDE+ G +HL+ ++
Sbjct: 806 KLIEVERLEQWEEVTVGAHVTTFPQLQVLEISKCPRLLSISDLFGCCDEN-GVQHLRVLQ 865

Query: 241 IRFCDQLKELTNGIQSCHSIQHLVLDKGSNLWSFVTTTTRGGPLAHLLFQRPINFFERR- 300
           I  C +L +L NG+Q CHSIQHL L       SF+    +   L   L +R  +  E + 
Sbjct: 866 ILECSKLTKLPNGLQFCHSIQHLRLIDNC---SFLALDLQNEHLLMRLPERLAHLSELKE 925

Query: 301 --------HGSFMNKR 304
                   HG  MN R
Sbjct: 926 MDIVGCTQHGDLMNLR 937

BLAST of Chy5G103350 vs. TAIR 10
Match: AT3G14470.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 106.7 bits (265), Expect = 3.5e-23
Identity = 75/227 (33.04%), Postives = 107/227 (47.14%), Query Frame = 0

Query: 1   MPLYLSELFQLKTLSAFVVGLKKGCTTTELGSLRKLKGLLVILCLEHVGSKEEAKSAKLV 60
           MP     L  L+TL+ F V    G   +ELG L  L G L I+ L+ V    +A  A L 
Sbjct: 664 MPRRFGRLKSLQTLTTFFVSASDGSRISELGGLHDLHGKLKIVELQRVVDVADAAEANLN 723

Query: 61  EKEKVEELHFVWSTESDRIVKN------ENDFEVLEGLEPHNNLKHLEIESFSGGQFPNQ 120
            K+ + E+ FVW T S     N      +N+ EV E L PH +++ L IE + G +FP+ 
Sbjct: 724 SKKHLREIDFVWRTGSSSSENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDW 783

Query: 121 I---FVENLVKITLIECGNCEKLPMLGQLTNLEILVIFRLRKVESIGNEFYGNQR----G 180
           +       +V I L EC  C  LP LGQL  L+ L I  +  ++SIG +FY + +     
Sbjct: 784 LSDPSFSRIVCIRLRECQYCTSLPSLGQLPCLKELHISGMVGLQSIGRKFYFSDQQLRDQ 843

Query: 181 SSSVFPKLKEFYVDEMDSLVEWEEAVSNNVKAFPRLECLHIISCKKL 215
               F  L+    D +    EW +        FP L+ L I+ C +L
Sbjct: 844 DQQPFRSLETLRFDNLPDWQEWLDVRVTRGDLFPSLKKLFILRCPEL 890