Chy5G102870 (gene) Cucumber (hystrix) v1

Overview
NameChy5G102870
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptioncalcium uptake protein, mitochondrial-like
LocationchrH05: 16795606 .. 16798140 (-)
RNA-Seq ExpressionChy5G102870
SyntenyChy5G102870
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTTCTAAAACGTTTTTGGTGTCAAAATCTTCTAAGCCAATTTGTAGAGTTATACCTTCTCAACACCTTACTGTTCGTTTTCTCGACAATCAACTTGGTGGCTCTTTAGGATCGCCATCAACATCCCCGTTGTCTTCTTCTTCTTCGTCAATTACATCTCAACAAACTGGCTGCGGAGAAGGCGAGAATAGAAGAAGCTTTGTATTTGACTTCTTGATCAAATTGATTTCTTCAGGGATATTGATTGGCGGAGTGGGATCACTTCTCAGCTCGAATTCTTCTGCGTCCAATTCGCATTTATCGTTTGCTGACTTCCCTAAGGAAACAACATGGACAACAGTGAAAGAAGATCAATTCCAATACCCATCGTCACCAAATCATCAAAATATAAGTCCAAAGAAGAAATCCAAATTTCTATTTGGAGGTAAGAGTGTTCCCTTTTTGCCATTAATCTAATAAATGTGTTTAGGGTAGAGTTTACCATTTTATTCATACTTTATACTGGACTCCAGATGATTATAGGAGAAGGGTGTTTTTCAACTATGAAAAACGTATAAGAATCCAAAGTCCTCCTGAGAAGGTATTTGAATATTTTGCCTCTATCCACACACCTGAAGGGGAAATATATATGAGACCATCAGATCTGATGAGGGCCATTGTTCCTGTGTTTCCTCCATCTGAGTCCAATCGGGTTAGAGAAGGCTTTTTAAGAGGAGAAAGAAGAATTTCTGGAGAACTATGTTGTGCTCCTTCTACCTTTTTCATGCTCTTCGACACCAACAATGATGGCCTCATCTCTTTTGCAGAGTAAGAATTCTTCGACCCTTCCAACTTCATGATCATTTACACAACCTGACATTCACATATAGTTGAACTTCATTCAAACTTAAATAAATACCTAATGCTTTTATTATAATTTTTAGTTTATGATTTATTTCCGGTGAGCTATCTAATAAATTGTTGAATTATAATGGCCAAACCTATGGGATCAAAACCAAAAGATCATGCATTGTGCTCTCGTCTCTTCGGTTTGCAGGTACATATTTTTTGTTACACTTCTCAGCATACCAGAATCCAGCTTTTCAGTGGCATTCAAAATGTTTGACATTGATAACAATGGGTTAGATAGTTAATTGCTTCTTCAAATTCGTCATTTTCCACATTTGTTTCCTCTTCTATTCACAATCCTCTTGGGTAATGAATGTTATCTGTCTTTGTTGATAAAATAGTGAAATAGACAGGGAGGAGTTCAAGAAAGTGATGGGTTTGATGAGAAAACAGAATAGACAGGGAGCTCACCACAGAGATGGAAGAAGGTTTGGGATGAAAGTTAGTGTTGAAAATGGTGGTTTAGTAGAATACTTCTTTGGTCAAGATGGAAAAGGCTCCCTTCACCATGACAAATTTGTCCAATTCCTAAGACAACTTCATGATGAGGTATGTCATTCCCCCAAATATCAATTCTTCGTGTACTTTTAACTCTTTTGAACTCAAAGACACAATTGTACAGTCTATAAAACAAAACCTGTTCAAATTCAGAACAGTTCCGTTTCAAATTCAAGCAAGGTATTTCTTCCTATACGTAACTCTTTAGAACTCAAAGTCACGGCTGACGGCTGTACAACCTATAGAACAGATGTATGGAAATTCAAGACTGTTATTCCTGTGCTCAACTTTTTAAACTCAAAGATTACGGTTGTACAAAGTATAGAAAAGATCTATTCAAATTCATGCAAGGTTGCTCTTCCTAACTCTTTAGACTCAAAGACACGATTGTACAATCTATACAAAAGATCCCTATTCAAATCCTTCCAATACTTAACTCTTTATAAATAAAAAACATGATTGGAGAAACTATAAAGCAGATCCTATTCAAATTCAGGTAAGGTCTTCTACCTACACATGACTCAACCTACATAAATTGGATGAATGCAGATACTTCTACTAGAGTTTTCACATTATGACTTCAAATCACAAGGAAGCATATCAGCCAAAGATTTTGCACTATCAATAGTTGCTTCAGCAGACATAAATCACATAGACAAACTACTGGACAGAGTTGAAGCATTAAACAAAGAACCCCATTTCAAAAACATCAGAATCACATTTGATGAATTCAAAGACTTTGCAGAGCTACGCAAGAAATTAGAATCCTTCTCTTTGGCTATTTTCAGCTATGGTAAAGTAAATGGAGAATTAACAAAACAAGATTTCCAAAGAGCAGCTTCCCATGTAAGTACACACCAAACATTTCAAGTCAACAAACTTAAAATCAATCTAATACAAAATGTGGGTTTTGTTTTTCTGTACAAGGTGTGTGGAGTTTCAATCACTAAGAATGTTGTGGACATAATATTTCACATATTTGATGCAAATGAAGATGGGGATTTAAGCTCAGATGAGTTCGTAAGAGTCATACAAAGAAGAGAAGTGAACTCTACACAACCAGCTGTTGGTGTTGGAGGATTACTGTCTTGTTGGTTTAGCTGTGCAGCTAAATGTTCTTACGCAAAGCTCTTTGTTCGCAGCTGA

mRNA sequence

ATGTTTTCTAAAACGTTTTTGGTGTCAAAATCTTCTAAGCCAATTTGTAGAGTTATACCTTCTCAACACCTTACTGTTCGTTTTCTCGACAATCAACTTGGTGGCTCTTTAGGATCGCCATCAACATCCCCGTTGTCTTCTTCTTCTTCGTCAATTACATCTCAACAAACTGGCTGCGGAGAAGGCGAGAATAGAAGAAGCTTTGTATTTGACTTCTTGATCAAATTGATTTCTTCAGGGATATTGATTGGCGGAGTGGGATCACTTCTCAGCTCGAATTCTTCTGCGTCCAATTCGCATTTATCGTTTGCTGACTTCCCTAAGGAAACAACATGGACAACAGTGAAAGAAGATCAATTCCAATACCCATCGTCACCAAATCATCAAAATATAAGTCCAAAGAAGAAATCCAAATTTCTATTTGGAGATGATTATAGGAGAAGGGTGTTTTTCAACTATGAAAAACGTATAAGAATCCAAAGTCCTCCTGAGAAGGTATTTGAATATTTTGCCTCTATCCACACACCTGAAGGGGAAATATATATGAGACCATCAGATCTGATGAGGGCCATTGTTCCTGTGTTTCCTCCATCTGAGTCCAATCGGGTTAGAGAAGGCTTTTTAAGAGGAGAAAGAAGAATTTCTGGAGAACTATGTTGTGCTCCTTCTACCTTTTTCATGCTCTTCGACACCAACAATGATGGCCTCATCTCTTTTGCAGAGTACATATTTTTTGTTACACTTCTCAGCATACCAGAATCCAGCTTTTCAGTGGCATTCAAAATGTTTGACATTGATAACAATGGTGAAATAGACAGGGAGGAGTTCAAGAAAGTGATGGGTTTGATGAGAAAACAGAATAGACAGGGAGCTCACCACAGAGATGGAAGAAGGTTTGGGATGAAAGTTAGTGTTGAAAATGGTGGTTTAGTAGAATACTTCTTTGGTCAAGATGGAAAAGGCTCCCTTCACCATGACAAATTTGTCCAATTCCTAAGACAACTTCATGATGAGATACTTCTACTAGAGTTTTCACATTATGACTTCAAATCACAAGGAAGCATATCAGCCAAAGATTTTGCACTATCAATAGTTGCTTCAGCAGACATAAATCACATAGACAAACTACTGGACAGAGTTGAAGCATTAAACAAAGAACCCCATTTCAAAAACATCAGAATCACATTTGATGAATTCAAAGACTTTGCAGAGCTACGCAAGAAATTAGAATCCTTCTCTTTGGCTATTTTCAGCTATGGTAAAGTAAATGGAGAATTAACAAAACAAGATTTCCAAAGAGCAGCTTCCCATGTGTGTGGAGTTTCAATCACTAAGAATGTTGTGGACATAATATTTCACATATTTGATGCAAATGAAGATGGGGATTTAAGCTCAGATGAGTTCGTAAGAGTCATACAAAGAAGAGAAGTGAACTCTACACAACCAGCTGTTGGTGTTGGAGGATTACTGTCTTGTTGGTTTAGCTGTGCAGCTAAATGTTCTTACGCAAAGCTCTTTGTTCGCAGCTGA

Coding sequence (CDS)

ATGTTTTCTAAAACGTTTTTGGTGTCAAAATCTTCTAAGCCAATTTGTAGAGTTATACCTTCTCAACACCTTACTGTTCGTTTTCTCGACAATCAACTTGGTGGCTCTTTAGGATCGCCATCAACATCCCCGTTGTCTTCTTCTTCTTCGTCAATTACATCTCAACAAACTGGCTGCGGAGAAGGCGAGAATAGAAGAAGCTTTGTATTTGACTTCTTGATCAAATTGATTTCTTCAGGGATATTGATTGGCGGAGTGGGATCACTTCTCAGCTCGAATTCTTCTGCGTCCAATTCGCATTTATCGTTTGCTGACTTCCCTAAGGAAACAACATGGACAACAGTGAAAGAAGATCAATTCCAATACCCATCGTCACCAAATCATCAAAATATAAGTCCAAAGAAGAAATCCAAATTTCTATTTGGAGATGATTATAGGAGAAGGGTGTTTTTCAACTATGAAAAACGTATAAGAATCCAAAGTCCTCCTGAGAAGGTATTTGAATATTTTGCCTCTATCCACACACCTGAAGGGGAAATATATATGAGACCATCAGATCTGATGAGGGCCATTGTTCCTGTGTTTCCTCCATCTGAGTCCAATCGGGTTAGAGAAGGCTTTTTAAGAGGAGAAAGAAGAATTTCTGGAGAACTATGTTGTGCTCCTTCTACCTTTTTCATGCTCTTCGACACCAACAATGATGGCCTCATCTCTTTTGCAGAGTACATATTTTTTGTTACACTTCTCAGCATACCAGAATCCAGCTTTTCAGTGGCATTCAAAATGTTTGACATTGATAACAATGGTGAAATAGACAGGGAGGAGTTCAAGAAAGTGATGGGTTTGATGAGAAAACAGAATAGACAGGGAGCTCACCACAGAGATGGAAGAAGGTTTGGGATGAAAGTTAGTGTTGAAAATGGTGGTTTAGTAGAATACTTCTTTGGTCAAGATGGAAAAGGCTCCCTTCACCATGACAAATTTGTCCAATTCCTAAGACAACTTCATGATGAGATACTTCTACTAGAGTTTTCACATTATGACTTCAAATCACAAGGAAGCATATCAGCCAAAGATTTTGCACTATCAATAGTTGCTTCAGCAGACATAAATCACATAGACAAACTACTGGACAGAGTTGAAGCATTAAACAAAGAACCCCATTTCAAAAACATCAGAATCACATTTGATGAATTCAAAGACTTTGCAGAGCTACGCAAGAAATTAGAATCCTTCTCTTTGGCTATTTTCAGCTATGGTAAAGTAAATGGAGAATTAACAAAACAAGATTTCCAAAGAGCAGCTTCCCATGTGTGTGGAGTTTCAATCACTAAGAATGTTGTGGACATAATATTTCACATATTTGATGCAAATGAAGATGGGGATTTAAGCTCAGATGAGTTCGTAAGAGTCATACAAAGAAGAGAAGTGAACTCTACACAACCAGCTGTTGGTGTTGGAGGATTACTGTCTTGTTGGTTTAGCTGTGCAGCTAAATGTTCTTACGCAAAGCTCTTTGTTCGCAGCTGA

Protein sequence

MFSKTFLVSKSSKPICRVIPSQHLTVRFLDNQLGGSLGSPSTSPLSSSSSSITSQQTGCGEGENRRSFVFDFLIKLISSGILIGGVGSLLSSNSSASNSHLSFADFPKETTWTTVKEDQFQYPSSPNHQNISPKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYMRPSDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSTFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMKVSVENGGLVEYFFGQDGKGSLHHDKFVQFLRQLHDEILLLEFSHYDFKSQGSISAKDFALSIVASADINHIDKLLDRVEALNKEPHFKNIRITFDEFKDFAELRKKLESFSLAIFSYGKVNGELTKQDFQRAASHVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVNSTQPAVGVGGLLSCWFSCAAKCSYAKLFVRS*
Homology
BLAST of Chy5G102870 vs. ExPASy Swiss-Prot
Match: Q9SZ45 (Calcium uptake protein, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MICU PE=2 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 7.0e-135
Identity = 272/476 (57.14%), Postives = 342/476 (71.85%), Query Frame = 0

Query: 38  GSPSTSPLSSSSSSITSQQTGCGEGENRRSFVFDFLIKLISSGILIGGVGSLLSSNSSAS 97
           GS S+ P SS S+SI S     G    + +     L K I +GI  G     L  +S +S
Sbjct: 30  GSSSSFPESSPSASILS-----GADPLKCTVSGGSLAKWI-TGISAGSALGFLYWSSGSS 89

Query: 98  NS---------HLSFADFPKETTWTTVKEDQFQYPSSPNHQNISPKKKSKFLFGDDYRRR 157
           +S          LSFAD    +    VK    + P S   +   P   S F+FGD YRR+
Sbjct: 90  DSISGLFGGSNLLSFADSSTPSV-CGVKVGDLK-PRSFIPKLSLPGYSSGFIFGDAYRRK 149

Query: 158 VFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYMRPSDLMRAIVPVFPPSESNRVREGFL 217
           +FFNYEKR+R+QSPPEKVFEYFAS+ T +GEI M+P+DLMRAIVPVFPPSES+ VREG+L
Sbjct: 150 IFFNYEKRLRLQSPPEKVFEYFASVRTDKGEILMKPADLMRAIVPVFPPSESHLVREGYL 209

Query: 218 RGERRISGELCCAPSTFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAFKMFDIDNN 277
            GER   GEL C+PS FFMLFD +NDGLISF EYIFFVTLLSIPESSF+VAFKMFD DNN
Sbjct: 210 TGERN-PGELRCSPSEFFMLFDVDNDGLISFKEYIFFVTLLSIPESSFAVAFKMFDTDNN 269

Query: 278 GEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMKV--SVENGGLVEYFFGQDGKGSLHHD 337
           GEID+EEFK VM LMR Q+RQG  HRDG R G+ +  SVE+GGLVEYFFG+DG   L HD
Sbjct: 270 GEIDKEEFKTVMSLMRSQHRQGVGHRDGLRTGLHMTGSVEDGGLVEYFFGKDGSQKLKHD 329

Query: 338 KFVQFLRQLHDEILLLEFSHYDFKSQGSISAKDFALSIVASADINHIDKLLDRVEALNKE 397
           KF +F++ L +E+L LEF+HYD+K +GSISAKDFALS+VA+AD +H+ KLLDRVE+L++ 
Sbjct: 330 KFTKFMKDLTEEMLRLEFAHYDYKRRGSISAKDFALSMVAAADASHLSKLLDRVESLSEH 389

Query: 398 PHFKNIRITFDEFKDFAELRKKLESFSLAIFSYGKVNGELTKQDFQRAASHVCGVSITKN 457
           PH +++RI+  EFK F ELR KL  FSLA+F+YGK NG LT +DF+RAAS VCG++++ N
Sbjct: 390 PHLRDMRISLKEFKQFDELRSKLGPFSLALFAYGKANGLLTMKDFKRAASQVCGITLSDN 449

Query: 458 VVDIIFHIFDANEDGDLSSDEFVRVIQRREVNSTQP-AVGVGGLLSCWFSCAAKCS 502
           V++I FH+FD+N+DG+LS DEF+RV+ RRE +  QP A G+    S  +  +  CS
Sbjct: 450 VIEIAFHVFDSNQDGNLSVDEFLRVLHRRERDVAQPIAKGLSRYFSDGWKGSKNCS 496

BLAST of Chy5G102870 vs. ExPASy Swiss-Prot
Match: B1H2N3 (Calcium uptake protein 1, mitochondrial OS=Xenopus tropicalis OX=8364 GN=micu1 PE=2 SV=2)

HSP 1 Score: 191.8 bits (486), Expect = 1.9e-47
Identity = 126/405 (31.11%), Postives = 208/405 (51.36%), Query Frame = 0

Query: 108 KETTWTTVKEDQFQYPSSPNHQNISPKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVF 167
           +E T    KED  Q   S +       KK K   G  +R R    YE RIR  S P+K+F
Sbjct: 68  REETSEKEKEDADQAVESSDEDQPQEGKKKKARVG--FRDRKVMEYENRIRAYSTPDKIF 127

Query: 168 EYFASI----HTPEGEIYMRPSDLMRAIVPVFPPSESNRVREGFLR--GERRISGELCCA 227
            YFA++     + E E++M P D +R+I P     E+  + +  ++    ++IS E    
Sbjct: 128 RYFATLKVIHESGESEVFMTPQDFVRSITPNEKQPENLGLDQFIIKRYDGKKISQEREKF 187

Query: 228 PSTFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMG 287
                + +     GLISF++YIF  T+LS P+ +F +AFKMFD++ +GE+D EEF++V  
Sbjct: 188 ADEDSIFYSLGECGLISFSDYIFLTTVLSTPQRNFEIAFKMFDLNGDGEVDMEEFEQVQS 247

Query: 288 LMRKQNRQGAHHRDGRRFGMKVSVE-NGGLVEYFFGQDGKGSLHHDKFVQFLRQLHDEIL 347
           ++R Q   G  HRD    G  +    +  L  YFFG D KG L    F++F R+L  ++L
Sbjct: 248 IIRSQTSMGMRHRDRSTTGNTLKTGFSSALTTYFFGADLKGKLTIKNFLEFQRKLQHDVL 307

Query: 348 LLEFSHYDFKSQGSISAKDFALSIVASADINHIDKLLDRVEALNKEPHFKNIR-ITFDEF 407
            LEF  +D    G I+ + F   ++A + +    KL   ++ L K   FK+   +TF+E 
Sbjct: 308 KLEFERHD-PVDGHITERQFGSMLLAYSGVQS-KKLTHMLKQLKK--RFKDAEGLTFEEV 367

Query: 408 KDFAELRKKLESFSLAIFSYGKVNGELTKQDFQRAASHVCGVSITKNVVDIIFHIFDANE 467
           ++F    K +     A+  Y      L K   Q+ A  V  V ++ +V D++F +FD + 
Sbjct: 368 ENFFTFLKNINDVDTALSFYHMAGASLDKVTMQQVARTVAKVELSDHVCDVVFALFDCDG 427

Query: 468 DGDLSSDEFVRVIQRREVNSTQPA--VGVGGLLSCWFSCAAKCSY 503
           +G+LS+ EF+ ++++R +   +    +G   L+   + CA + ++
Sbjct: 428 NGELSNKEFIAIMKQRLMRGLEKPKDMGFTRLMRAMWKCAQETAW 466

BLAST of Chy5G102870 vs. ExPASy Swiss-Prot
Match: Q95PZ2 (Calcium uptake protein 1 homolog, mitochondrial OS=Caenorhabditis elegans OX=6239 GN=micu-1 PE=2 SV=2)

HSP 1 Score: 184.1 bits (466), Expect = 3.9e-45
Identity = 122/360 (33.89%), Postives = 185/360 (51.39%), Query Frame = 0

Query: 129 QNISPKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASI-------HTPEGEIY 188
           + +  K K K L    +R R    YE R+R+ S P+K+F YFA++        +   E++
Sbjct: 147 EEVKEKPKKKKL---GFRERRIIEYEDRLRLYSTPDKIFRYFATLKIIDPNEDSGRFEVF 206

Query: 189 MRPSDLMRAIVP-VFPPSESNRVREGFLRGERRISGELCCAPSTFFMLFDTNNDGLISFA 248
           M P D +R+  P V  P             E+    +     S F+ L     +GLI+F+
Sbjct: 207 MTPEDFLRSFTPGVMQPRRWGLDSFKNYNPEKHKRHKFSDPDSIFYKL---GENGLINFS 266

Query: 249 EYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFG 308
           +Y+F +TLLS   + F++AFK+FD+D NG +D+EEF KV  L+  Q   G  HRD     
Sbjct: 267 DYLFLMTLLSTSHADFALAFKIFDVDGNGALDKEEFTKVQQLIMSQTTVGQRHRDHITPN 326

Query: 309 MKVSVE-NGGLVEYFFGQDGKGSLHHDKFVQFLRQLHDEILLLEFSHYDF--KSQGSISA 368
               VE N  L  YFFG+DGKGSL  +KF++F  +L  +IL +EF   D      G I+ 
Sbjct: 327 QSFRVETNSALETYFFGKDGKGSLSSEKFIEFQERLQHDILKMEFERRDALDNPDGLINE 386

Query: 369 KDFALSIVASADINHIDK--LLDRVEALNKEPHFKNIRITFDEFKDFAELRKKLESFSLA 428
             FA  ++  A IN   +  +L RV+   K  + K   I+F E K F E    ++   +A
Sbjct: 387 DSFAQLLLLHAQINEKKQKHMLKRVKRRFKGENLKG--ISFGETKAFFEFLYHIDDVDIA 446

Query: 429 IFSYGKVNGELTKQDFQRAASHVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRR 476
           +  +      +  +  QR A  V G+ ++ +VVD++  +FD N DG LS +E V V++RR
Sbjct: 447 LHFHKMAGMSIDAKLLQRVAVKVTGIPLSDHVVDVVITLFDDNLDGKLSHEEMVAVMRRR 498

BLAST of Chy5G102870 vs. ExPASy Swiss-Prot
Match: Q4R518 (Calcium uptake protein 1, mitochondrial OS=Macaca fascicularis OX=9541 GN=MICU1 PE=2 SV=2)

HSP 1 Score: 184.1 bits (466), Expect = 3.9e-45
Identity = 124/390 (31.79%), Postives = 202/390 (51.79%), Query Frame = 0

Query: 127 NHQ----NISPKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFAS---IHTP-EG 186
           NH+    +++P  + K      +R R    YE RIR  S P+K+F YFA+   I+ P E 
Sbjct: 84  NHEKKTADLAPHPEEKKKKRSGFRDRKVMEYENRIRAYSTPDKIFRYFATLKVINEPGEA 143

Query: 187 EIYMRPSDLMRAIVPVFPPSESNRVREGFLR--GERRISGELCCAPSTFFMLFDTNNDGL 246
           E++M P D +R+I P     E   + +  ++    ++IS E         + +     GL
Sbjct: 144 EVFMTPEDFVRSITPNEKQPEHLGLDQYIIKRFDGKKISQEREKFADEGSIFYTLGECGL 203

Query: 247 ISFAEYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDG 306
           ISF++YIF  T+LS P+ +F +AFKMFD++ +GE+D EEF++V  ++R Q   G  HRD 
Sbjct: 204 ISFSDYIFLTTVLSTPQRNFEIAFKMFDLNGDGEVDMEEFEQVQSIIRSQTSMGMRHRDR 263

Query: 307 RRFGMKV-SVENGGLVEYFFGQDGKGSLHHDKFVQFLRQLHDEILLLEFSHYDFKSQGSI 366
              G  + S     L  YFFG D KG L    F++F R+L  ++L LEF  +D    G I
Sbjct: 264 PTTGNTLKSGLCSALTTYFFGADLKGKLTIKNFLEFQRKLQHDVLKLEFERHD-PVDGRI 323

Query: 367 SAKDFALSIVASADINHIDKLLDRVEALNKEPHFKNIR-ITFDEFKDFAELRKKLESFSL 426
           + + F   ++A + +    KL      L K  HFK  + +TF E ++F    K +     
Sbjct: 324 TERQFGGMLLAYSGVQS-KKLTAMQRQLKK--HFKEGKGLTFQEVENFFTFLKNINDVDT 383

Query: 427 AIFSYGKVNGELTKQDFQRAASHVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQR 486
           A+  Y      L K   Q+ A  V  V ++ +V D++F +FD + +G+LS+ EFV ++++
Sbjct: 384 ALSFYHMAGASLDKVTMQQVARTVAKVELSDHVCDVVFALFDCDGNGELSNKEFVSIMKQ 443

Query: 487 REVNSTQPA--VGVGGLLSCWFSCAAKCSY 503
           R +   +    +G   L+   + CA + ++
Sbjct: 444 RLMRGLEKPKDMGFTRLMQAMWKCAQETAW 469

BLAST of Chy5G102870 vs. ExPASy Swiss-Prot
Match: Q6P6Q9 (Calcium uptake protein 1, mitochondrial OS=Rattus norvegicus OX=10116 GN=Micu1 PE=2 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 3.9e-45
Identity = 124/380 (32.63%), Postives = 200/380 (52.63%), Query Frame = 0

Query: 133 PKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFAS---IHTP-EGEIYMRPSDLM 192
           P++K K   G  +R R    YE RIR  S P+K+F YFA+   I+ P E E++M P D +
Sbjct: 98  PEEKKKKRSG--FRDRKVMEYENRIRAYSTPDKIFRYFATLKVINEPGETEVFMTPQDFV 157

Query: 193 RAIVPVFPPSESNRVREGFLR--GERRISGELCCAPSTFFMLFDTNNDGLISFAEYIFFV 252
           R+I P     E   + +  ++    ++I+ E         + +     GLISF++YIF  
Sbjct: 158 RSITPNEKQPEHLGLDQYIIKRFDGKKIAQEREKFADEGSIFYTLGECGLISFSDYIFLT 217

Query: 253 TLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMKV-SV 312
           T+LS P+ +F +AFKMFD++ +GE+D EEF++V  ++R Q   G  HRD    G  + S 
Sbjct: 218 TVLSTPQRNFEIAFKMFDLNGDGEVDMEEFEQVQSIIRSQTSMGMRHRDRPTTGNTLKSG 277

Query: 313 ENGGLVEYFFGQDGKGSLHHDKFVQFLRQLHDEILLLEFSHYDFKSQGSISAKDFALSIV 372
               L  YFFG D KG L    F++F R+L  ++L LEF  +D    G IS + F   ++
Sbjct: 278 LCSALTTYFFGADLKGKLTIKNFLEFQRKLQHDVLKLEFERHD-PVDGRISERQFGGMLL 337

Query: 373 ASADINHIDKLLDRVEALNKEPHFKNIR-ITFDEFKDFAELRKKLESFSLAIFSYGKVNG 432
           A + +    KL      L K  HFK+ + +TF E ++F    K +     A+  Y     
Sbjct: 338 AYSGVQS-KKLTAMQRQLKK--HFKDGKGLTFQEVENFFTFLKNINDVDTALSFYHMAGA 397

Query: 433 ELTKQDFQRAASHVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVNSTQPA- 492
            L K   Q+ A  V  V ++ +V D++F +FD + +G+LS+ EFV ++++R +   +   
Sbjct: 398 SLDKVTMQQVARTVAKVELSDHVCDVVFALFDCDGNGELSNKEFVSIMKQRLMRGLEKPK 457

Query: 493 -VGVGGLLSCWFSCAAKCSY 503
            +G   L+   + CA + ++
Sbjct: 458 DMGFTRLMQAMWKCAQETAW 471

BLAST of Chy5G102870 vs. ExPASy TrEMBL
Match: A0A0A0LJ54 (Calcium ion binding protein OS=Cucumis sativus OX=3659 GN=Csa_2G009450 PE=4 SV=1)

HSP 1 Score: 989.9 bits (2558), Expect = 3.8e-285
Identity = 501/511 (98.04%), Postives = 504/511 (98.63%), Query Frame = 0

Query: 1   MFSKTFLVSKSSKPICRVIPSQHLTVRFLDNQLGGSLGSPSTSPL--SSSSSSITSQQTG 60
           MFS TFLVSKSSKPICRVIPSQHLTVRFLDNQLGGSLGSPSTSPL  SSSSSSITSQQTG
Sbjct: 1   MFSSTFLVSKSSKPICRVIPSQHLTVRFLDNQLGGSLGSPSTSPLFSSSSSSSITSQQTG 60

Query: 61  CGEGENRRSFVFDFLIKLISSGILIGGVGSLLSSNSSASNSHLSFADFPKETTWTTVKED 120
           CG GENRRSFVFDFLIKLISSGILIGGVGSLLSSNSSASNSHLSFADFPKETTWTTVKED
Sbjct: 61  CGGGENRRSFVFDFLIKLISSGILIGGVGSLLSSNSSASNSHLSFADFPKETTWTTVKED 120

Query: 121 QFQYPSSPNHQNISPKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEG 180
           QFQYPSSPNHQNI+PKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEG
Sbjct: 121 QFQYPSSPNHQNINPKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEG 180

Query: 181 EIYMRPSDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSTFFMLFDTNNDGLIS 240
           EIYMRPSDLMRAIVPVFPPSESNRVREGFLRGERR+SGELCCAPSTFFMLFDTNNDGLIS
Sbjct: 181 EIYMRPSDLMRAIVPVFPPSESNRVREGFLRGERRMSGELCCAPSTFFMLFDTNNDGLIS 240

Query: 241 FAEYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRR 300
           FAEYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHR+GRR
Sbjct: 241 FAEYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHREGRR 300

Query: 301 FGMKVSVENGGLVEYFFGQDGKGSLHHDKFVQFLRQLHDEILLLEFSHYDFKSQGSISAK 360
           FGMKVSVENGGLVEYFFGQDGK SLHHDKFVQFLRQLHDEILLLEFSHYDFKSQGSISAK
Sbjct: 301 FGMKVSVENGGLVEYFFGQDGKASLHHDKFVQFLRQLHDEILLLEFSHYDFKSQGSISAK 360

Query: 361 DFALSIVASADINHIDKLLDRVEALNKEPHFKNIRITFDEFKDFAELRKKLESFSLAIFS 420
           DFALSIVASADINHIDKLLDRVEALNKEPHFKNIRITFDEFKDFAELRKKLESFSLAIFS
Sbjct: 361 DFALSIVASADINHIDKLLDRVEALNKEPHFKNIRITFDEFKDFAELRKKLESFSLAIFS 420

Query: 421 YGKVNGELTKQDFQRAASHVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVN 480
           YGKVNGELTKQDFQRAASHVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVN
Sbjct: 421 YGKVNGELTKQDFQRAASHVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVN 480

Query: 481 STQPAVGVGGLLSCWFSCAAKCSYAKLFVRS 510
           STQPAVG GGLLSCWF CAAKCSYAKLFVRS
Sbjct: 481 STQPAVGAGGLLSCWFGCAAKCSYAKLFVRS 511

BLAST of Chy5G102870 vs. ExPASy TrEMBL
Match: A0A5A7VDG3 (Calcium uptake protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G001000 PE=4 SV=1)

HSP 1 Score: 946.4 bits (2445), Expect = 4.8e-272
Identity = 485/509 (95.28%), Postives = 497/509 (97.64%), Query Frame = 0

Query: 1   MFSKTFLVSKSSKPICRVIPSQHLTVRFLDNQLGGSLGSPSTSPLSSSSSSITSQQTGCG 60
           MFS TFLVSKSSK ICRVIPSQHLTVRFL+NQLGGSLGSPSTSPL SSSSSIT+QQTGCG
Sbjct: 1   MFSSTFLVSKSSKSICRVIPSQHLTVRFLNNQLGGSLGSPSTSPL-SSSSSITTQQTGCG 60

Query: 61  EGENRRSFVFDFLIKLISSGILIGGVGSLLSSNSSASNSHLSFADFPKETTWTTVKEDQF 120
            GENRR+FVF+FLIKLISSGILIGGVGSLLSSNSS SNSHLSFA+FPKETTWTTVKEDQF
Sbjct: 61  -GENRRNFVFEFLIKLISSGILIGGVGSLLSSNSSTSNSHLSFAEFPKETTWTTVKEDQF 120

Query: 121 QYPSSPNHQNISPKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEI 180
           QYP SPN QNI+ KKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEI
Sbjct: 121 QYP-SPN-QNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEI 180

Query: 181 YMRPSDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSTFFMLFDTNNDGLISFA 240
           YMRPSDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPS FFMLFDTNNDGLISFA
Sbjct: 181 YMRPSDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFA 240

Query: 241 EYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFG 300
           EYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFG
Sbjct: 241 EYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFG 300

Query: 301 MKVSVENGGLVEYFFGQDGKGSLHHDKFVQFLRQLHDEILLLEFSHYDFKSQGSISAKDF 360
           MKVSVENGGLVEYFFG+DGKGSLHHDKFVQFLRQLH+EIL+LEFSHYDFKSQGSIS KDF
Sbjct: 301 MKVSVENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDF 360

Query: 361 ALSIVASADINHIDKLLDRVEALNKEPHFKNIRITFDEFKDFAELRKKLESFSLAIFSYG 420
           ALSIVASADINHIDKLLDRVEALNKEPHFKNIRIT++EFKDFAELRKKLESFSLAIFSYG
Sbjct: 361 ALSIVASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYG 420

Query: 421 KVNGELTKQDFQRAASHVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVNST 480
           KVNGELTKQDFQRAAS VCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVNS+
Sbjct: 421 KVNGELTKQDFQRAASQVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVNSS 480

Query: 481 QPAVGVGGLLSCWFSCAAKCSYAKLFVRS 510
           QPAVGVGGLLSCWFSCAAKCS AKLFVRS
Sbjct: 481 QPAVGVGGLLSCWFSCAAKCSSAKLFVRS 505

BLAST of Chy5G102870 vs. ExPASy TrEMBL
Match: A0A1S3C4U2 (calcium uptake protein 1, mitochondrial-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103496476 PE=4 SV=1)

HSP 1 Score: 944.9 bits (2441), Expect = 1.4e-271
Identity = 484/509 (95.09%), Postives = 496/509 (97.45%), Query Frame = 0

Query: 1   MFSKTFLVSKSSKPICRVIPSQHLTVRFLDNQLGGSLGSPSTSPLSSSSSSITSQQTGCG 60
           MFS TFLVSKSSK ICRVIPSQHLTVRFL+NQLGGSLGSPSTSPL SSSSSIT+QQTGCG
Sbjct: 1   MFSSTFLVSKSSKSICRVIPSQHLTVRFLNNQLGGSLGSPSTSPL-SSSSSITTQQTGCG 60

Query: 61  EGENRRSFVFDFLIKLISSGILIGGVGSLLSSNSSASNSHLSFADFPKETTWTTVKEDQF 120
            GENRR+FVF+FLIKLISSG+LIGGVGSLLSS SS SNSHLSFADFPKETTWTTVKEDQF
Sbjct: 61  -GENRRNFVFEFLIKLISSGLLIGGVGSLLSSKSSTSNSHLSFADFPKETTWTTVKEDQF 120

Query: 121 QYPSSPNHQNISPKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEI 180
           QYP SPN QNI+ KKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEI
Sbjct: 121 QYP-SPN-QNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEI 180

Query: 181 YMRPSDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSTFFMLFDTNNDGLISFA 240
           YMRPSDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPS FFMLFDTNNDGLISFA
Sbjct: 181 YMRPSDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFA 240

Query: 241 EYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFG 300
           EYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFG
Sbjct: 241 EYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFG 300

Query: 301 MKVSVENGGLVEYFFGQDGKGSLHHDKFVQFLRQLHDEILLLEFSHYDFKSQGSISAKDF 360
           MKVSVENGGLVEYFFG+DGKGSLHHDKFVQFLRQLH+EIL+LEFSHYDFKSQGSIS KDF
Sbjct: 301 MKVSVENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDF 360

Query: 361 ALSIVASADINHIDKLLDRVEALNKEPHFKNIRITFDEFKDFAELRKKLESFSLAIFSYG 420
           ALSIVASADINHIDKLLDRVEALNKEPHFKNIRIT++EFKDFAELRKKLESFSLAIFSYG
Sbjct: 361 ALSIVASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYG 420

Query: 421 KVNGELTKQDFQRAASHVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVNST 480
           KVNGELTKQDFQRAAS VCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVNS+
Sbjct: 421 KVNGELTKQDFQRAASQVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVNSS 480

Query: 481 QPAVGVGGLLSCWFSCAAKCSYAKLFVRS 510
           QPAVGVGGLLSCWFSCAAKCS AKLFVRS
Sbjct: 481 QPAVGVGGLLSCWFSCAAKCSSAKLFVRS 505

BLAST of Chy5G102870 vs. ExPASy TrEMBL
Match: A0A1S3C3I8 (calcium uptake protein 1, mitochondrial-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103496476 PE=4 SV=1)

HSP 1 Score: 930.2 bits (2403), Expect = 3.6e-267
Identity = 484/536 (90.30%), Postives = 496/536 (92.54%), Query Frame = 0

Query: 1   MFSKTFLVSKSSKPICRVIPSQHLTVRFLDNQLGGSLGSPSTSPLSSSSSSITSQQTGCG 60
           MFS TFLVSKSSK ICRVIPSQHLTVRFL+NQLGGSLGSPSTSPL SSSSSIT+QQTGCG
Sbjct: 1   MFSSTFLVSKSSKSICRVIPSQHLTVRFLNNQLGGSLGSPSTSPL-SSSSSITTQQTGCG 60

Query: 61  EGENRRSFVFDFLIKLISSGILIGGVGSLLSSNSSASNSHLSFADFPKETTWTTVKEDQF 120
            GENRR+FVF+FLIKLISSG+LIGGVGSLLSS SS SNSHLSFADFPKETTWTTVKEDQF
Sbjct: 61  -GENRRNFVFEFLIKLISSGLLIGGVGSLLSSKSSTSNSHLSFADFPKETTWTTVKEDQF 120

Query: 121 QYPSSPNHQNISPKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEI 180
           QYP SPN QNI+ KKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEI
Sbjct: 121 QYP-SPN-QNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEI 180

Query: 181 YMRPSDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSTFFMLFDTNNDGLISFA 240
           YMRPSDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPS FFMLFDTNNDGLISFA
Sbjct: 181 YMRPSDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFA 240

Query: 241 EYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFG 300
           EYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFG
Sbjct: 241 EYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFG 300

Query: 301 MKVSVENGGLVEYFFGQDGKGSLHHDKFVQFLRQLHDEILLLEFSHYDFKSQGSISAKDF 360
           MKVSVENGGLVEYFFG+DGKGSLHHDKFVQFLRQLH+EIL+LEFSHYDFKSQGSIS KDF
Sbjct: 301 MKVSVENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDF 360

Query: 361 ALSIVASADINHIDKLLDRVEALNKEPHFKNIRITFDEFKDFAELRKKLESFSLAIFSYG 420
           ALSIVASADINHIDKLLDRVEALNKEPHFKNIRIT++EFKDFAELRKKLESFSLAIFSYG
Sbjct: 361 ALSIVASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYG 420

Query: 421 KVNGELTKQDFQRAASH---------------------------VCGVSITKNVVDIIFH 480
           KVNGELTKQDFQRAAS                            VCGVSITKNVVDIIFH
Sbjct: 421 KVNGELTKQDFQRAASQVSTHQTFQVNKLKINLIHNVGFVFLYKVCGVSITKNVVDIIFH 480

Query: 481 IFDANEDGDLSSDEFVRVIQRREVNSTQPAVGVGGLLSCWFSCAAKCSYAKLFVRS 510
           IFDANEDGDLSSDEFVRVIQRREVNS+QPAVGVGGLLSCWFSCAAKCS AKLFVRS
Sbjct: 481 IFDANEDGDLSSDEFVRVIQRREVNSSQPAVGVGGLLSCWFSCAAKCSSAKLFVRS 532

BLAST of Chy5G102870 vs. ExPASy TrEMBL
Match: A0A6J1CPF9 (calcium uptake protein, mitochondrial-like OS=Momordica charantia OX=3673 GN=LOC111013100 PE=4 SV=1)

HSP 1 Score: 805.4 bits (2079), Expect = 1.3e-229
Identity = 422/509 (82.91%), Postives = 451/509 (88.61%), Query Frame = 0

Query: 1   MFSKTFLVSKSSKPICRVIPSQHLTVRFLDNQLGGSLGSPSTSPLSSSSSSITSQQTGCG 60
           MFS T LVSKSS  I RV+ SQ LTVR  +NQLG   GS STSP  SSS   +S  TG G
Sbjct: 1   MFSSTLLVSKSSASIHRVVTSQRLTVRLFNNQLG---GSASTSP--SSSLLASSHNTGSG 60

Query: 61  EGENRRSFVFDFLIKLISSGILIGGVGSLLSSNSSASNSHLSFADFPKETTWTTVKEDQF 120
           E E++RS +FDFL+K +SSG++  G+GSLL  NSS SNS++SFADF KET W T+KEDQF
Sbjct: 61  E-ESKRSSIFDFLVKSVSSGVVAVGIGSLLCLNSSTSNSNVSFADFQKETKW-TMKEDQF 120

Query: 121 QYPSSPNHQNISPKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEI 180
           QYP      N + +KKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFAS+HTPEGEI
Sbjct: 121 QYP----FPNQNARKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASVHTPEGEI 180

Query: 181 YMRPSDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSTFFMLFDTNNDGLISFA 240
           +M P+DLMRAIVPVFPPSESNRVREGFLRGE RI GEL C PS FFMLFD N+DGLISFA
Sbjct: 181 FMTPADLMRAIVPVFPPSESNRVREGFLRGE-RIHGELYCVPSEFFMLFDINSDGLISFA 240

Query: 241 EYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFG 300
           EYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVM LMRKQNRQGA HRDGRR G
Sbjct: 241 EYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMALMRKQNRQGARHRDGRRLG 300

Query: 301 MKVSVENGGLVEYFFGQDGKGSLHHDKFVQFLRQLHDEILLLEFSHYDFKSQGSISAKDF 360
           MKV VENGGLVEYFFGQDGKGSLHHDKFVQFLRQLHDEIL LEFSHYDFKSQGSISAKDF
Sbjct: 301 MKVCVENGGLVEYFFGQDGKGSLHHDKFVQFLRQLHDEILRLEFSHYDFKSQGSISAKDF 360

Query: 361 ALSIVASADINHIDKLLDRVEALNKEPHFKNIRITFDEFKDFAELRKKLESFSLAIFSYG 420
           ALS+VASADINHIDKLLDRVEALNKEP F+NIRIT++EFKDFAELRKKLESFSLAIFSYG
Sbjct: 361 ALSMVASADINHIDKLLDRVEALNKEPQFRNIRITYEEFKDFAELRKKLESFSLAIFSYG 420

Query: 421 KVNGELTKQDFQRAASHVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVNST 480
           KVNGELTKQDFQRAASHVCGVSIT+NVVDIIFHIFDAN DGDLSSDEFVRVIQR+EV+S+
Sbjct: 421 KVNGELTKQDFQRAASHVCGVSITRNVVDIIFHIFDANGDGDLSSDEFVRVIQRKEVSSS 480

Query: 481 QPAVGVGGLLSCWFSCAAKCSYAKLFVRS 510
           Q AVG GG LSCWFSC+AKCS AKLFVRS
Sbjct: 481 QAAVGAGGFLSCWFSCSAKCSSAKLFVRS 497

BLAST of Chy5G102870 vs. NCBI nr
Match: XP_004139397.1 (calcium uptake protein, mitochondrial [Cucumis sativus] >KGN60757.1 hypothetical protein Csa_019233 [Cucumis sativus])

HSP 1 Score: 986 bits (2549), Expect = 0.0
Identity = 501/511 (98.04%), Postives = 504/511 (98.63%), Query Frame = 0

Query: 1   MFSKTFLVSKSSKPICRVIPSQHLTVRFLDNQLGGSLGSPSTSPL--SSSSSSITSQQTG 60
           MFS TFLVSKSSKPICRVIPSQHLTVRFLDNQLGGSLGSPSTSPL  SSSSSSITSQQTG
Sbjct: 1   MFSSTFLVSKSSKPICRVIPSQHLTVRFLDNQLGGSLGSPSTSPLFSSSSSSSITSQQTG 60

Query: 61  CGEGENRRSFVFDFLIKLISSGILIGGVGSLLSSNSSASNSHLSFADFPKETTWTTVKED 120
           CG GENRRSFVFDFLIKLISSGILIGGVGSLLSSNSSASNSHLSFADFPKETTWTTVKED
Sbjct: 61  CGGGENRRSFVFDFLIKLISSGILIGGVGSLLSSNSSASNSHLSFADFPKETTWTTVKED 120

Query: 121 QFQYPSSPNHQNISPKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEG 180
           QFQYPSSPNHQNI+PKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEG
Sbjct: 121 QFQYPSSPNHQNINPKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEG 180

Query: 181 EIYMRPSDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSTFFMLFDTNNDGLIS 240
           EIYMRPSDLMRAIVPVFPPSESNRVREGFLRGERR+SGELCCAPSTFFMLFDTNNDGLIS
Sbjct: 181 EIYMRPSDLMRAIVPVFPPSESNRVREGFLRGERRMSGELCCAPSTFFMLFDTNNDGLIS 240

Query: 241 FAEYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRR 300
           FAEYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHR+GRR
Sbjct: 241 FAEYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHREGRR 300

Query: 301 FGMKVSVENGGLVEYFFGQDGKGSLHHDKFVQFLRQLHDEILLLEFSHYDFKSQGSISAK 360
           FGMKVSVENGGLVEYFFGQDGK SLHHDKFVQFLRQLHDEILLLEFSHYDFKSQGSISAK
Sbjct: 301 FGMKVSVENGGLVEYFFGQDGKASLHHDKFVQFLRQLHDEILLLEFSHYDFKSQGSISAK 360

Query: 361 DFALSIVASADINHIDKLLDRVEALNKEPHFKNIRITFDEFKDFAELRKKLESFSLAIFS 420
           DFALSIVASADINHIDKLLDRVEALNKEPHFKNIRITFDEFKDFAELRKKLESFSLAIFS
Sbjct: 361 DFALSIVASADINHIDKLLDRVEALNKEPHFKNIRITFDEFKDFAELRKKLESFSLAIFS 420

Query: 421 YGKVNGELTKQDFQRAASHVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVN 480
           YGKVNGELTKQDFQRAASHVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVN
Sbjct: 421 YGKVNGELTKQDFQRAASHVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVN 480

Query: 481 STQPAVGVGGLLSCWFSCAAKCSYAKLFVRS 509
           STQPAVG GGLLSCWF CAAKCSYAKLFVRS
Sbjct: 481 STQPAVGAGGLLSCWFGCAAKCSYAKLFVRS 511

BLAST of Chy5G102870 vs. NCBI nr
Match: KAA0063559.1 (calcium uptake protein 1 [Cucumis melo var. makuwa] >TYJ97885.1 calcium uptake protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 942 bits (2436), Expect = 0.0
Identity = 485/509 (95.28%), Postives = 497/509 (97.64%), Query Frame = 0

Query: 1   MFSKTFLVSKSSKPICRVIPSQHLTVRFLDNQLGGSLGSPSTSPLSSSSSSITSQQTGCG 60
           MFS TFLVSKSSK ICRVIPSQHLTVRFL+NQLGGSLGSPSTSPLSSSSS IT+QQTGCG
Sbjct: 1   MFSSTFLVSKSSKSICRVIPSQHLTVRFLNNQLGGSLGSPSTSPLSSSSS-ITTQQTGCG 60

Query: 61  EGENRRSFVFDFLIKLISSGILIGGVGSLLSSNSSASNSHLSFADFPKETTWTTVKEDQF 120
            GENRR+FVF+FLIKLISSGILIGGVGSLLSSNSS SNSHLSFA+FPKETTWTTVKEDQF
Sbjct: 61  -GENRRNFVFEFLIKLISSGILIGGVGSLLSSNSSTSNSHLSFAEFPKETTWTTVKEDQF 120

Query: 121 QYPSSPNHQNISPKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEI 180
           QYPS PN QNI+ KKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEI
Sbjct: 121 QYPS-PN-QNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEI 180

Query: 181 YMRPSDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSTFFMLFDTNNDGLISFA 240
           YMRPSDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPS FFMLFDTNNDGLISFA
Sbjct: 181 YMRPSDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFA 240

Query: 241 EYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFG 300
           EYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFG
Sbjct: 241 EYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFG 300

Query: 301 MKVSVENGGLVEYFFGQDGKGSLHHDKFVQFLRQLHDEILLLEFSHYDFKSQGSISAKDF 360
           MKVSVENGGLVEYFFG+DGKGSLHHDKFVQFLRQLH+EIL+LEFSHYDFKSQGSIS KDF
Sbjct: 301 MKVSVENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDF 360

Query: 361 ALSIVASADINHIDKLLDRVEALNKEPHFKNIRITFDEFKDFAELRKKLESFSLAIFSYG 420
           ALSIVASADINHIDKLLDRVEALNKEPHFKNIRIT++EFKDFAELRKKLESFSLAIFSYG
Sbjct: 361 ALSIVASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYG 420

Query: 421 KVNGELTKQDFQRAASHVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVNST 480
           KVNGELTKQDFQRAAS VCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVNS+
Sbjct: 421 KVNGELTKQDFQRAASQVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVNSS 480

Query: 481 QPAVGVGGLLSCWFSCAAKCSYAKLFVRS 509
           QPAVGVGGLLSCWFSCAAKCS AKLFVRS
Sbjct: 481 QPAVGVGGLLSCWFSCAAKCSSAKLFVRS 505

BLAST of Chy5G102870 vs. NCBI nr
Match: XP_008456571.1 (PREDICTED: calcium uptake protein 1, mitochondrial-like isoform X2 [Cucumis melo])

HSP 1 Score: 941 bits (2432), Expect = 0.0
Identity = 484/509 (95.09%), Postives = 496/509 (97.45%), Query Frame = 0

Query: 1   MFSKTFLVSKSSKPICRVIPSQHLTVRFLDNQLGGSLGSPSTSPLSSSSSSITSQQTGCG 60
           MFS TFLVSKSSK ICRVIPSQHLTVRFL+NQLGGSLGSPSTSPLSSSSS IT+QQTGCG
Sbjct: 1   MFSSTFLVSKSSKSICRVIPSQHLTVRFLNNQLGGSLGSPSTSPLSSSSS-ITTQQTGCG 60

Query: 61  EGENRRSFVFDFLIKLISSGILIGGVGSLLSSNSSASNSHLSFADFPKETTWTTVKEDQF 120
            GENRR+FVF+FLIKLISSG+LIGGVGSLLSS SS SNSHLSFADFPKETTWTTVKEDQF
Sbjct: 61  -GENRRNFVFEFLIKLISSGLLIGGVGSLLSSKSSTSNSHLSFADFPKETTWTTVKEDQF 120

Query: 121 QYPSSPNHQNISPKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEI 180
           QYPS PN QNI+ KKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEI
Sbjct: 121 QYPS-PN-QNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEI 180

Query: 181 YMRPSDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSTFFMLFDTNNDGLISFA 240
           YMRPSDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPS FFMLFDTNNDGLISFA
Sbjct: 181 YMRPSDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFA 240

Query: 241 EYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFG 300
           EYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFG
Sbjct: 241 EYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFG 300

Query: 301 MKVSVENGGLVEYFFGQDGKGSLHHDKFVQFLRQLHDEILLLEFSHYDFKSQGSISAKDF 360
           MKVSVENGGLVEYFFG+DGKGSLHHDKFVQFLRQLH+EIL+LEFSHYDFKSQGSIS KDF
Sbjct: 301 MKVSVENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDF 360

Query: 361 ALSIVASADINHIDKLLDRVEALNKEPHFKNIRITFDEFKDFAELRKKLESFSLAIFSYG 420
           ALSIVASADINHIDKLLDRVEALNKEPHFKNIRIT++EFKDFAELRKKLESFSLAIFSYG
Sbjct: 361 ALSIVASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYG 420

Query: 421 KVNGELTKQDFQRAASHVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVNST 480
           KVNGELTKQDFQRAAS VCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVNS+
Sbjct: 421 KVNGELTKQDFQRAASQVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVNSS 480

Query: 481 QPAVGVGGLLSCWFSCAAKCSYAKLFVRS 509
           QPAVGVGGLLSCWFSCAAKCS AKLFVRS
Sbjct: 481 QPAVGVGGLLSCWFSCAAKCSSAKLFVRS 505

BLAST of Chy5G102870 vs. NCBI nr
Match: XP_008456564.1 (PREDICTED: calcium uptake protein 1, mitochondrial-like isoform X1 [Cucumis melo])

HSP 1 Score: 926 bits (2394), Expect = 0.0
Identity = 484/536 (90.30%), Postives = 496/536 (92.54%), Query Frame = 0

Query: 1   MFSKTFLVSKSSKPICRVIPSQHLTVRFLDNQLGGSLGSPSTSPLSSSSSSITSQQTGCG 60
           MFS TFLVSKSSK ICRVIPSQHLTVRFL+NQLGGSLGSPSTSPLSSSSS IT+QQTGCG
Sbjct: 1   MFSSTFLVSKSSKSICRVIPSQHLTVRFLNNQLGGSLGSPSTSPLSSSSS-ITTQQTGCG 60

Query: 61  EGENRRSFVFDFLIKLISSGILIGGVGSLLSSNSSASNSHLSFADFPKETTWTTVKEDQF 120
            GENRR+FVF+FLIKLISSG+LIGGVGSLLSS SS SNSHLSFADFPKETTWTTVKEDQF
Sbjct: 61  -GENRRNFVFEFLIKLISSGLLIGGVGSLLSSKSSTSNSHLSFADFPKETTWTTVKEDQF 120

Query: 121 QYPSSPNHQNISPKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEI 180
           QYPS PN QNI+ KKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEI
Sbjct: 121 QYPS-PN-QNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEI 180

Query: 181 YMRPSDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSTFFMLFDTNNDGLISFA 240
           YMRPSDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPS FFMLFDTNNDGLISFA
Sbjct: 181 YMRPSDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFA 240

Query: 241 EYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFG 300
           EYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFG
Sbjct: 241 EYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFG 300

Query: 301 MKVSVENGGLVEYFFGQDGKGSLHHDKFVQFLRQLHDEILLLEFSHYDFKSQGSISAKDF 360
           MKVSVENGGLVEYFFG+DGKGSLHHDKFVQFLRQLH+EIL+LEFSHYDFKSQGSIS KDF
Sbjct: 301 MKVSVENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDF 360

Query: 361 ALSIVASADINHIDKLLDRVEALNKEPHFKNIRITFDEFKDFAELRKKLESFSLAIFSYG 420
           ALSIVASADINHIDKLLDRVEALNKEPHFKNIRIT++EFKDFAELRKKLESFSLAIFSYG
Sbjct: 361 ALSIVASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYG 420

Query: 421 KVNGELTKQDFQRAASHV---------------------------CGVSITKNVVDIIFH 480
           KVNGELTKQDFQRAAS V                           CGVSITKNVVDIIFH
Sbjct: 421 KVNGELTKQDFQRAASQVSTHQTFQVNKLKINLIHNVGFVFLYKVCGVSITKNVVDIIFH 480

Query: 481 IFDANEDGDLSSDEFVRVIQRREVNSTQPAVGVGGLLSCWFSCAAKCSYAKLFVRS 509
           IFDANEDGDLSSDEFVRVIQRREVNS+QPAVGVGGLLSCWFSCAAKCS AKLFVRS
Sbjct: 481 IFDANEDGDLSSDEFVRVIQRREVNSSQPAVGVGGLLSCWFSCAAKCSSAKLFVRS 532

BLAST of Chy5G102870 vs. NCBI nr
Match: XP_022143166.1 (calcium uptake protein, mitochondrial-like [Momordica charantia])

HSP 1 Score: 801 bits (2069), Expect = 4.54e-289
Identity = 422/509 (82.91%), Postives = 451/509 (88.61%), Query Frame = 0

Query: 1   MFSKTFLVSKSSKPICRVIPSQHLTVRFLDNQLGGSLGSPSTSPLSSSSSSITSQQTGCG 60
           MFS T LVSKSS  I RV+ SQ LTVR  +NQLGGS    STSP  SSS   +S  TG G
Sbjct: 1   MFSSTLLVSKSSASIHRVVTSQRLTVRLFNNQLGGSA---STSP--SSSLLASSHNTGSG 60

Query: 61  EGENRRSFVFDFLIKLISSGILIGGVGSLLSSNSSASNSHLSFADFPKETTWTTVKEDQF 120
           E E++RS +FDFL+K +SSG++  G+GSLL  NSS SNS++SFADF KET WT +KEDQF
Sbjct: 61  E-ESKRSSIFDFLVKSVSSGVVAVGIGSLLCLNSSTSNSNVSFADFQKETKWT-MKEDQF 120

Query: 121 QYPSSPNHQNISPKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEI 180
           QYP      N + +KKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFAS+HTPEGEI
Sbjct: 121 QYP----FPNQNARKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASVHTPEGEI 180

Query: 181 YMRPSDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSTFFMLFDTNNDGLISFA 240
           +M P+DLMRAIVPVFPPSESNRVREGFLRGER I GEL C PS FFMLFD N+DGLISFA
Sbjct: 181 FMTPADLMRAIVPVFPPSESNRVREGFLRGER-IHGELYCVPSEFFMLFDINSDGLISFA 240

Query: 241 EYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFG 300
           EYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVM LMRKQNRQGA HRDGRR G
Sbjct: 241 EYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMALMRKQNRQGARHRDGRRLG 300

Query: 301 MKVSVENGGLVEYFFGQDGKGSLHHDKFVQFLRQLHDEILLLEFSHYDFKSQGSISAKDF 360
           MKV VENGGLVEYFFGQDGKGSLHHDKFVQFLRQLHDEIL LEFSHYDFKSQGSISAKDF
Sbjct: 301 MKVCVENGGLVEYFFGQDGKGSLHHDKFVQFLRQLHDEILRLEFSHYDFKSQGSISAKDF 360

Query: 361 ALSIVASADINHIDKLLDRVEALNKEPHFKNIRITFDEFKDFAELRKKLESFSLAIFSYG 420
           ALS+VASADINHIDKLLDRVEALNKEP F+NIRIT++EFKDFAELRKKLESFSLAIFSYG
Sbjct: 361 ALSMVASADINHIDKLLDRVEALNKEPQFRNIRITYEEFKDFAELRKKLESFSLAIFSYG 420

Query: 421 KVNGELTKQDFQRAASHVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVNST 480
           KVNGELTKQDFQRAASHVCGVSIT+NVVDIIFHIFDAN DGDLSSDEFVRVIQR+EV+S+
Sbjct: 421 KVNGELTKQDFQRAASHVCGVSITRNVVDIIFHIFDANGDGDLSSDEFVRVIQRKEVSSS 480

Query: 481 QPAVGVGGLLSCWFSCAAKCSYAKLFVRS 509
           Q AVG GG LSCWFSC+AKCS AKLFVRS
Sbjct: 481 QAAVGAGGFLSCWFSCSAKCSSAKLFVRS 497

BLAST of Chy5G102870 vs. TAIR 10
Match: AT4G32060.1 (calcium-binding EF hand family protein )

HSP 1 Score: 482.3 bits (1240), Expect = 5.0e-136
Identity = 272/476 (57.14%), Postives = 342/476 (71.85%), Query Frame = 0

Query: 38  GSPSTSPLSSSSSSITSQQTGCGEGENRRSFVFDFLIKLISSGILIGGVGSLLSSNSSAS 97
           GS S+ P SS S+SI S     G    + +     L K I +GI  G     L  +S +S
Sbjct: 30  GSSSSFPESSPSASILS-----GADPLKCTVSGGSLAKWI-TGISAGSALGFLYWSSGSS 89

Query: 98  NS---------HLSFADFPKETTWTTVKEDQFQYPSSPNHQNISPKKKSKFLFGDDYRRR 157
           +S          LSFAD    +    VK    + P S   +   P   S F+FGD YRR+
Sbjct: 90  DSISGLFGGSNLLSFADSSTPSV-CGVKVGDLK-PRSFIPKLSLPGYSSGFIFGDAYRRK 149

Query: 158 VFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYMRPSDLMRAIVPVFPPSESNRVREGFL 217
           +FFNYEKR+R+QSPPEKVFEYFAS+ T +GEI M+P+DLMRAIVPVFPPSES+ VREG+L
Sbjct: 150 IFFNYEKRLRLQSPPEKVFEYFASVRTDKGEILMKPADLMRAIVPVFPPSESHLVREGYL 209

Query: 218 RGERRISGELCCAPSTFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAFKMFDIDNN 277
            GER   GEL C+PS FFMLFD +NDGLISF EYIFFVTLLSIPESSF+VAFKMFD DNN
Sbjct: 210 TGERN-PGELRCSPSEFFMLFDVDNDGLISFKEYIFFVTLLSIPESSFAVAFKMFDTDNN 269

Query: 278 GEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMKV--SVENGGLVEYFFGQDGKGSLHHD 337
           GEID+EEFK VM LMR Q+RQG  HRDG R G+ +  SVE+GGLVEYFFG+DG   L HD
Sbjct: 270 GEIDKEEFKTVMSLMRSQHRQGVGHRDGLRTGLHMTGSVEDGGLVEYFFGKDGSQKLKHD 329

Query: 338 KFVQFLRQLHDEILLLEFSHYDFKSQGSISAKDFALSIVASADINHIDKLLDRVEALNKE 397
           KF +F++ L +E+L LEF+HYD+K +GSISAKDFALS+VA+AD +H+ KLLDRVE+L++ 
Sbjct: 330 KFTKFMKDLTEEMLRLEFAHYDYKRRGSISAKDFALSMVAAADASHLSKLLDRVESLSEH 389

Query: 398 PHFKNIRITFDEFKDFAELRKKLESFSLAIFSYGKVNGELTKQDFQRAASHVCGVSITKN 457
           PH +++RI+  EFK F ELR KL  FSLA+F+YGK NG LT +DF+RAAS VCG++++ N
Sbjct: 390 PHLRDMRISLKEFKQFDELRSKLGPFSLALFAYGKANGLLTMKDFKRAASQVCGITLSDN 449

Query: 458 VVDIIFHIFDANEDGDLSSDEFVRVIQRREVNSTQP-AVGVGGLLSCWFSCAAKCS 502
           V++I FH+FD+N+DG+LS DEF+RV+ RRE +  QP A G+    S  +  +  CS
Sbjct: 450 VIEIAFHVFDSNQDGNLSVDEFLRVLHRRERDVAQPIAKGLSRYFSDGWKGSKNCS 496

BLAST of Chy5G102870 vs. TAIR 10
Match: AT4G32060.2 (calcium-binding EF hand family protein )

HSP 1 Score: 422.2 bits (1084), Expect = 6.1e-118
Identity = 245/427 (57.38%), Postives = 303/427 (70.96%), Query Frame = 0

Query: 38  GSPSTSPLSSSSSSITSQQTGCGEGENRRSFVFDFLIKLISSGILIGGVGSLLSSNSSAS 97
           GS S+ P SS S+SI S     G    + +     L K I +GI  G     L  +S +S
Sbjct: 30  GSSSSFPESSPSASILS-----GADPLKCTVSGGSLAKWI-TGISAGSALGFLYWSSGSS 89

Query: 98  NS---------HLSFADFPKETTWTTVKEDQFQYPSSPNHQNISPKKKSKFLFGDDYRRR 157
           +S          LSFAD    +    VK    + P S   +   P   S F+FGD YRR+
Sbjct: 90  DSISGLFGGSNLLSFADSSTPSV-CGVKVGDLK-PRSFIPKLSLPGYSSGFIFGDAYRRK 149

Query: 158 VFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYMRPSDLMRAIVPVFPPSESNRVREGFL 217
           +FFNYEKR+R+QSPPEKVFEYFAS+ T +GEI M+P+DLMRAIVPVFPPSES+ VREG+L
Sbjct: 150 IFFNYEKRLRLQSPPEKVFEYFASVRTDKGEILMKPADLMRAIVPVFPPSESHLVREGYL 209

Query: 218 RGERRISGELCCAPSTFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAFKMFDIDNN 277
            GER   GEL C+PS FFMLFD +NDGLISF EYIFFVTLLSIPESSF+VAFKMFD DNN
Sbjct: 210 TGERN-PGELRCSPSEFFMLFDVDNDGLISFKEYIFFVTLLSIPESSFAVAFKMFDTDNN 269

Query: 278 GEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMKV--SVENGGLVEYFFGQDGKGSLHHD 337
           GEID+EEFK VM LMR Q+RQG  HRDG R G+ +  SVE+GGLVEYFFG+DG   L HD
Sbjct: 270 GEIDKEEFKTVMSLMRSQHRQGVGHRDGLRTGLHMTGSVEDGGLVEYFFGKDGSQKLKHD 329

Query: 338 KFVQFLRQLHDEILLLEFSHYDFKSQGSISAKDFALSIVASADINHIDKLLDRVEALNKE 397
           KF +F++ L +E+L LEF+HYD+K +GSISAKDFALS+VA+AD +H+ KLLDRVE+L++ 
Sbjct: 330 KFTKFMKDLTEEMLRLEFAHYDYKRRGSISAKDFALSMVAAADASHLSKLLDRVESLSEH 389

Query: 398 PHFKNIRITFDEFKDFAELRKKLESFSLAIFSYGKVNGELTKQDFQRAAS--HVCGVSIT 452
           PH +++RI+  EFK F ELR KL  FSLA+F+YGK NG LT +DF+RAAS     G +  
Sbjct: 390 PHLRDMRISLKEFKQFDELRSKLGPFSLALFAYGKANGLLTMKDFKRAASQREGRGTANC 447

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SZ457.0e-13557.14Calcium uptake protein, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MICU PE... [more]
B1H2N31.9e-4731.11Calcium uptake protein 1, mitochondrial OS=Xenopus tropicalis OX=8364 GN=micu1 P... [more]
Q95PZ23.9e-4533.89Calcium uptake protein 1 homolog, mitochondrial OS=Caenorhabditis elegans OX=623... [more]
Q4R5183.9e-4531.79Calcium uptake protein 1, mitochondrial OS=Macaca fascicularis OX=9541 GN=MICU1 ... [more]
Q6P6Q93.9e-4532.63Calcium uptake protein 1, mitochondrial OS=Rattus norvegicus OX=10116 GN=Micu1 P... [more]
Match NameE-valueIdentityDescription
A0A0A0LJ543.8e-28598.04Calcium ion binding protein OS=Cucumis sativus OX=3659 GN=Csa_2G009450 PE=4 SV=1[more]
A0A5A7VDG34.8e-27295.28Calcium uptake protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A1S3C4U21.4e-27195.09calcium uptake protein 1, mitochondrial-like isoform X2 OS=Cucumis melo OX=3656 ... [more]
A0A1S3C3I83.6e-26790.30calcium uptake protein 1, mitochondrial-like isoform X1 OS=Cucumis melo OX=3656 ... [more]
A0A6J1CPF91.3e-22982.91calcium uptake protein, mitochondrial-like OS=Momordica charantia OX=3673 GN=LOC... [more]
Match NameE-valueIdentityDescription
XP_004139397.10.098.04calcium uptake protein, mitochondrial [Cucumis sativus] >KGN60757.1 hypothetical... [more]
KAA0063559.10.095.28calcium uptake protein 1 [Cucumis melo var. makuwa] >TYJ97885.1 calcium uptake p... [more]
XP_008456571.10.095.09PREDICTED: calcium uptake protein 1, mitochondrial-like isoform X2 [Cucumis melo... [more]
XP_008456564.10.090.30PREDICTED: calcium uptake protein 1, mitochondrial-like isoform X1 [Cucumis melo... [more]
XP_022143166.14.54e-28982.91calcium uptake protein, mitochondrial-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT4G32060.15.0e-13657.14calcium-binding EF hand family protein [more]
AT4G32060.26.1e-11857.38calcium-binding EF hand family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 447..475
e-value: 1.1
score: 17.4
coord: 255..283
e-value: 2.6E-5
score: 33.7
coord: 221..249
e-value: 0.43
score: 19.6
IPR002048EF-hand domainPFAMPF13833EF-hand_8coord: 226..247
e-value: 7.7E-4
score: 19.2
coord: 423..474
e-value: 5.0E-7
score: 29.4
IPR002048EF-hand domainPFAMPF00036EF-hand_1coord: 259..281
e-value: 9.0E-7
score: 28.0
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 443..478
score: 10.747526
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 251..286
score: 13.118635
IPR002048EF-hand domainCDDcd00051EFhcoord: 226..280
e-value: 5.28108E-11
score: 56.0169
NoneNo IPR availableGENE3D1.10.238.10coord: 340..483
e-value: 1.4E-11
score: 46.3
NoneNo IPR availableGENE3D1.10.238.10coord: 160..300
e-value: 1.7E-14
score: 55.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 37..61
NoneNo IPR availablePANTHERPTHR12294:SF23CALCIUM UPTAKE PROTEIN, MITOCHONDRIALcoord: 68..505
NoneNo IPR availableCDDcd15900EFh_MICUcoord: 256..475
e-value: 2.13321E-42
score: 146.219
IPR039800Calcium uptake protein 1/2/3PANTHERPTHR12294EF HAND DOMAIN FAMILY A1,A2-RELATEDcoord: 68..505
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 264..276
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 456..468
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 225..473

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy5G102870.1Chy5G102870.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0036444 calcium import into the mitochondrion
biological_process GO:0051560 mitochondrial calcium ion homeostasis
biological_process GO:0006851 mitochondrial calcium ion transmembrane transport
cellular_component GO:1990246 uniplex complex
molecular_function GO:0005509 calcium ion binding