Chy5G100700 (gene) Cucumber (hystrix) v1

Overview
NameChy5G100700
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionLipoyl(octanoyl) transferase
LocationchrH05: 14861354 .. 14864184 (-)
RNA-Seq ExpressionChy5G100700
SyntenyChy5G100700
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTATCTGAAATTGAAGAAGCAAATGATTGGTGGGAAAAACGTTAACTGGGTTTCATTTTATAGATACAATTAACGAGAAAAAGTAGGTAAAGTTGTCTAATTGGTGGATTAAGTGGTGGGTTTTTTTTCTCTCAAATTAGAAAAGTTTTTAAGTTTAATTTTGAGATTTTAAACAAATCTATAAGCCTTCTTTTAAGGGAATTGTTAGATTCTTCTCAAGTGGAAATGAATAACTCAAGTTTTTGCCTTTAGATTTTCCGCCTCCTTCAGTTCTCTCTCTTATTTGGGTCAATATTTAGCAAAATTACCTTTAAAAGGCAAAAGTATTTATGGATCTTTACATTTATATTTTACTTATCAATTTTAACATTAAATACCTAAAATATTTTGGACTGTAATTATTTTATTTTAAAATATTGATCAAATCGAACTCTAAATTTTCAACTTAAATGTATATTTATACACTCGAAACATAACACATAATATTTATTATATAGAAAATTGAAAGTTTATAGACATATAAATAACTAAGGACGTGAAAAACTTACCTCAAAACCTTTTGTAAGGTTTAGTTATATGTTTGGTATAACACAAATGTTATTAGTGACTGGTTGATTCAAATTTCCTACTCCTATTCAACTAGAAAAAGAAAATTAAGACTACCCAAATTTTTTACATAGAATAATTTGTAGGCAAGTATACTTCAACATGATAAAAATAATAATAAAATAAAGGTTCACATGTAAAAATCCTTTACGGACAGTTTGTCTCATACGCATCTTAGATTCTCATAATGTGGGCATTCAAATTGTTGTGATTGTTTTGCAATATTGTAAGATGTCTTGATACCTAAAGATTATATTCTCAAGCATCCTAGAACATCGGAATAGAGGACATCTTGAAAGTTTTTGATGCCCTCTTAAAACACGGCCACACACACATACTAAGATGTTGATAATTTTTCTACATCAAACATGATAATCTAGAGATGTCAAACACGCATAACTTCATATATATATCCTATAATTTCAAACATGGTCTTATATTCCTAAAAATATACAAACAGCTCCTTAATCCTTTTATTATAATCATTATTTTTCTCATAAACTCTCTCTATAGGAACCTTTTTCTACTGCAATAAATTAGATATTTTTAGAGATATATTCTTAAAAAAAAATAGATATTTTTATGAACTAGAACACTACGTGAACTAGAACATTACGTGTTATGTACACGTGTATTTTTAAATATATTACGTATTTTAATATAATATATGAGGTTCATATATTTAAGTAAATTGTGCAGAAGAGCAATGTTGAGAATAAGTGGATGTTCTTTGGCTTTTTCCTAAAAATTGCTACTACTTTCCATTCCAATAAGGATACAAGAGTACTGAATTTGGATGTCCATTTTTAATAGCTTTTCAATTTAAAGTATTCCTACAATACTCAGCTTTACATGTTCTCGTTCTATAGCTATTTTTCTTCCATTTTTGTTGGATGATCTTGTTGGTTACGTTGCTGTCACGAGAAAAAAAAAAAATAATAATTTTAGTGACATATATTCGGTACCAGGTTTGGATTCTGAAAAAGAAAAAAGAACTCATTTTAATCTTTGCTAGAGCACGATGGTTTTGTTAAAGTTCTCAACCATTTCTTGAATTTTTTTTTGAGATTAATGTTATATTGTTTTTCTGAATATACTCATGTTGAGATGGTTCGATGCTGATAACTTAGCCATGTGTGAATGAAGCCTTAGGATTGCAAGTTGTTCAGTTTGCTTGTTTTCAGGTACGGTGTGGATCTACATCAATCCTTTAAGCAATACATTTTGCTCTCCCTTCATTTTCATTTCTTGTTATGCAAATCAAGCACTCTCTAGAATCAATCTCAACAATTAAAAGCATATCATAGAGAGGAGATAAATTTTGATATTACTTCACAGATAGCCGAATCTAAATCCCCGAAATGCCCCCTTTGTGTCTATATATTCTTCCATTCCATTTATTAGATTCCAAACCACAGACATTAAGGAGGTGTTTGTTGGACTGTAATGGAACAGGAATGTAATCTAATGGAATAAAAACTCATATTTGGATGGGTCGTTTTGAGTCTAAATTGCAATACTAAACCTATTCTTTCTCATAGTTTTTAATTCAACCTTGTTTTCAAAAGTTTTCACACTTCACAATTACATTTTATTCGTTCCTCGCTCAATCTTCATGCATTCCAACATCCATCCAACATCTTTCTGATTTCAAGTAATCTGGATCACATTCTAACTCTTTAAACAGCTCCTAAGAAAGTACTTCATCTCCAAGAAAAATTCGTCTCTACTAGAAAATCTAGTAAAATCCCAGACACACTCTTAACCAACATACACACTTGGAAAAAGGCGAACTGATCATAATCTATTAATCCCTGTTCGATCTAGCGAAGCTAGGAACTGAACTTCACTACACTGAGAGAGGGGGAGACATTACATTTCATGGTCCAAATCAATCCATTTTGTACCCAATAATTTCCCTCAGAGATGTTGGATTTGGGGCTAGAAAGTATGTGGAGAAGCTAGAACGACAGACCATGGCATGTTTGTATGGAGTGAAAGCTAGAGCTGGGGAGAAAGGGGAAACGGGGGTTTGGGTTGGAGATCATAGGAAAATTGGAGCCATTGGAGTTAGAATTTCAAATGGGGTTACTTCTCATGGTTTAGCTTTTAATGTGGATCCTGATTTGATGTCTTTTATGCATATTGTGCCTTGTGGGATTGTTGATAAAGATGTGACATCTTTGAGAGAAGAGACTAAGGAAGTGCTTCCTAGTGAAAAA

mRNA sequence

ATGGTATCTGAAATTGAAGAAGCAAATGATTGGTGGGAAAAACCGAAGCTAGGAACTGAACTTCACTACACTGAGAGAGGGGGAGACATTACATTTCATGGTCCAAATCAATCCATTTTGTACCCAATAATTTCCCTCAGAGATGTTGGATTTGGGGCTAGAAAGTATGTGGAGAAGCTAGAACGACAGACCATGGCATGTTTGTATGGAGTGAAAGCTAGAGCTGGGGAGAAAGGGGAAACGGGGGTTTGGGTTGGAGATCATAGGAAAATTGGAGCCATTGGAGTTAGAATTTCAAATGGGGTTACTTCTCATGGTTTAGCTTTTAATGTGGATCCTGATTTGATGTCTTTTATGCATATTGTGCCTTGTGGGATTGTTGATAAAGATGTGACATCTTTGAGAGAAGAGACTAAGGAAGTGCTTCCTAGTGAAAAA

Coding sequence (CDS)

ATGGTATCTGAAATTGAAGAAGCAAATGATTGGTGGGAAAAACCGAAGCTAGGAACTGAACTTCACTACACTGAGAGAGGGGGAGACATTACATTTCATGGTCCAAATCAATCCATTTTGTACCCAATAATTTCCCTCAGAGATGTTGGATTTGGGGCTAGAAAGTATGTGGAGAAGCTAGAACGACAGACCATGGCATGTTTGTATGGAGTGAAAGCTAGAGCTGGGGAGAAAGGGGAAACGGGGGTTTGGGTTGGAGATCATAGGAAAATTGGAGCCATTGGAGTTAGAATTTCAAATGGGGTTACTTCTCATGGTTTAGCTTTTAATGTGGATCCTGATTTGATGTCTTTTATGCATATTGTGCCTTGTGGGATTGTTGATAAAGATGTGACATCTTTGAGAGAAGAGACTAAGGAAGTGCTTCCTAGTGAAAAA

Protein sequence

MVSEIEEANDWWEKPKLGTELHYTERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLERQTMACLYGVKARAGEKGETGVWVGDHRKIGAIGVRISNGVTSHGLAFNVDPDLMSFMHIVPCGIVDKDVTSLREETKEVLPSEK
Homology
BLAST of Chy5G100700 vs. ExPASy Swiss-Prot
Match: Q9SXP7 (Octanoyltransferase LIP2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=LIP2 PE=1 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 1.0e-51
Identity = 100/136 (73.53%), Postives = 114/136 (83.82%), Query Frame = 0

Query: 13  EKPKLGTELHYTERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLERQTM--ACLYG 72
           E  K+G ELHYT+RGGDITFHGP+Q+ILYPIISLR +GFGAR YVE LER  +  A +YG
Sbjct: 66  ELTKIGAELHYTQRGGDITFHGPHQAILYPIISLRSIGFGARNYVETLERSMIEFASIYG 125

Query: 73  VKARAGEKGETGVWVGDHRKIGAIGVRISNGVTSHGLAFNVDPDLMSFMHIVPCGIVDKD 132
           VKARAG K ETGVWVGD RKIGAIGVRIS+G+TSHGLA N+DPD+  F HIVPCGI DK+
Sbjct: 126 VKARAGNKCETGVWVGD-RKIGAIGVRISSGITSHGLALNIDPDMKYFEHIVPCGIADKE 185

Query: 133 VTSLREETKEVLPSEK 147
           VTSLR ET  +LPSE+
Sbjct: 186 VTSLRRETDTLLPSEE 200

BLAST of Chy5G100700 vs. ExPASy Swiss-Prot
Match: Q5LDM3 (Octanoyltransferase OS=Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343) OX=272559 GN=lipB PE=3 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 5.8e-26
Identity = 65/127 (51.18%), Postives = 84/127 (66.14%), Query Frame = 0

Query: 17  LGTELHYTERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLERQT--MACLYGVKAR 76
           +G  L++ +RGGDIT+HGP Q + YPI++L + G G ++YV  LE     +   YGV A 
Sbjct: 71  IGATLYHIDRGGDITYHGPGQLVCYPILNLEEFGLGLKEYVHLLEEAVIRVCASYGVVAG 130

Query: 77  AGEKGETGVWV-GD---HRKIGAIGVRISNGVTSHGLAFNVDPDLMSFMHIVPCGIVDKD 136
             EK  TGVW+ GD    RKI AIGVR S+ VT HGLA NV+ DL  F +I PCG +DK 
Sbjct: 131 RLEKA-TGVWLEGDTSRARKICAIGVRSSHYVTMHGLALNVNTDLRYFSYIHPCGFIDKG 190

Query: 137 VTSLREE 138
           VTSL++E
Sbjct: 191 VTSLQQE 196

BLAST of Chy5G100700 vs. ExPASy Swiss-Prot
Match: Q64UP8 (Octanoyltransferase OS=Bacteroides fragilis (strain YCH46) OX=295405 GN=lipB PE=3 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 5.8e-26
Identity = 65/127 (51.18%), Postives = 84/127 (66.14%), Query Frame = 0

Query: 17  LGTELHYTERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLERQT--MACLYGVKAR 76
           +G  L++ +RGGDIT+HGP Q + YPI++L + G G ++YV  LE     +   YGV A 
Sbjct: 71  IGATLYHIDRGGDITYHGPGQLVCYPILNLEEFGLGLKEYVHLLEEAVIRVCASYGVVAG 130

Query: 77  AGEKGETGVWV-GD---HRKIGAIGVRISNGVTSHGLAFNVDPDLMSFMHIVPCGIVDKD 136
             EK  TGVW+ GD    RKI AIGVR S+ VT HGLA NV+ DL  F +I PCG +DK 
Sbjct: 131 RLEKA-TGVWLEGDTSRARKICAIGVRSSHYVTMHGLALNVNTDLRYFSYIHPCGFIDKG 190

Query: 137 VTSLREE 138
           VTSL++E
Sbjct: 191 VTSLQQE 196

BLAST of Chy5G100700 vs. ExPASy Swiss-Prot
Match: Q8A8S8 (Octanoyltransferase OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482) OX=226186 GN=lipB PE=3 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 2.2e-25
Identity = 63/132 (47.73%), Postives = 84/132 (63.64%), Query Frame = 0

Query: 21  LHYTERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLERQT--MACLYGVKARAGEK 80
           L + +RGGDIT+HGP Q + YPI++L +   G ++YV  LE     +   YG++A   EK
Sbjct: 75  LFHIDRGGDITYHGPGQLVCYPILNLEEFHLGLKEYVHLLEEAVIRVCASYGIEAGRLEK 134

Query: 81  GETGVWVGDH----RKIGAIGVRISNGVTSHGLAFNVDPDLMSFMHIVPCGIVDKDVTSL 140
             TGVW+       RKI AIGVR S+ VT HGLA NV+ DL  F +I PCG +DK VTSL
Sbjct: 135 A-TGVWLEGSTPRARKICAIGVRSSHYVTMHGLALNVNTDLRYFSYIHPCGFIDKGVTSL 194

Query: 141 REETKEVLPSEK 147
           R+E K  +P ++
Sbjct: 195 RQELKHEVPMDE 205

BLAST of Chy5G100700 vs. ExPASy Swiss-Prot
Match: B8HGZ8 (Octanoyltransferase OS=Pseudarthrobacter chlorophenolicus (strain ATCC 700700 / DSM 12829 / CIP 107037 / JCM 12360 / KCTC 9906 / NCIMB 13794 / A6) OX=452863 GN=lipB PE=3 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 2.4e-24
Identity = 63/141 (44.68%), Postives = 87/141 (61.70%), Query Frame = 0

Query: 13  EKPKLGTELHYTERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLERQTMACL--YG 72
           E+P  GT +   +RGG +T+HGP Q I YPI+ L++   G R YVE+LE   +A +  YG
Sbjct: 59  ERPFDGTPVVAVDRGGKLTWHGPGQLIAYPILKLKNRA-GIRDYVERLEAVMIAVMADYG 118

Query: 73  VKARAGEKGETGVWV-----GDHRKIGAIGVRISNGVTSHGLAFNVDPDLMSFMHIVPCG 132
           ++A    KG  GVWV     G  RKI AIG+R+ NGVT HG+A N   DL  +  I+ CG
Sbjct: 119 IRAER-IKGRAGVWVKADANGPDRKIAAIGIRVLNGVTMHGVAINCSNDLAPYGQIIACG 178

Query: 133 IVDKDVTSLREET-KEVLPSE 146
           I D  VT++  ET K++ P++
Sbjct: 179 ITDAGVTTMSRETGKDIRPAD 197

BLAST of Chy5G100700 vs. ExPASy TrEMBL
Match: A0A5A7TLG4 (Lipoyl(octanoyl) transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold824G00760 PE=3 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 3.7e-60
Identity = 120/133 (90.23%), Postives = 124/133 (93.23%), Query Frame = 0

Query: 16  KLGTELHYTERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLERQ--TMACLYGVKA 75
           KLG ELHYTERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLE+    MACLYGVKA
Sbjct: 69  KLGAELHYTERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLEQTMIEMACLYGVKA 128

Query: 76  RAGEKGETGVWVGDHRKIGAIGVRISNGVTSHGLAFNVDPDLMSFMHIVPCGIVDKDVTS 135
           RAGEKGETGVWVGD RKIGAIGVRISNG+TSHGLAFNVDPDLM F HIVPCGIVDKDVTS
Sbjct: 129 RAGEKGETGVWVGD-RKIGAIGVRISNGITSHGLAFNVDPDLMYFKHIVPCGIVDKDVTS 188

Query: 136 LREETKEVLPSEK 147
           LR+ETKEVLP E+
Sbjct: 189 LRKETKEVLPGEE 200

BLAST of Chy5G100700 vs. ExPASy TrEMBL
Match: A0A5D3DTA6 (Lipoyl(octanoyl) transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold699G00090 PE=3 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 3.7e-60
Identity = 120/133 (90.23%), Postives = 124/133 (93.23%), Query Frame = 0

Query: 16  KLGTELHYTERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLERQ--TMACLYGVKA 75
           KLG ELHYTERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLE+    MACLYGVKA
Sbjct: 69  KLGAELHYTERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLEQTMIEMACLYGVKA 128

Query: 76  RAGEKGETGVWVGDHRKIGAIGVRISNGVTSHGLAFNVDPDLMSFMHIVPCGIVDKDVTS 135
           RAGEKGETGVWVGD RKIGAIGVRISNG+TSHGLAFNVDPDLM F HIVPCGIVDKDVTS
Sbjct: 129 RAGEKGETGVWVGD-RKIGAIGVRISNGITSHGLAFNVDPDLMYFKHIVPCGIVDKDVTS 188

Query: 136 LREETKEVLPSEK 147
           LR+ETKEVLP E+
Sbjct: 189 LRKETKEVLPGEE 200

BLAST of Chy5G100700 vs. ExPASy TrEMBL
Match: A0A1S3BQW0 (Lipoyl(octanoyl) transferase OS=Cucumis melo OX=3656 GN=LOC103492510 PE=3 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 3.7e-60
Identity = 120/133 (90.23%), Postives = 124/133 (93.23%), Query Frame = 0

Query: 16  KLGTELHYTERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLERQ--TMACLYGVKA 75
           KLG ELHYTERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLE+    MACLYGVKA
Sbjct: 69  KLGAELHYTERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLEQTMIEMACLYGVKA 128

Query: 76  RAGEKGETGVWVGDHRKIGAIGVRISNGVTSHGLAFNVDPDLMSFMHIVPCGIVDKDVTS 135
           RAGEKGETGVWVGD RKIGAIGVRISNG+TSHGLAFNVDPDLM F HIVPCGIVDKDVTS
Sbjct: 129 RAGEKGETGVWVGD-RKIGAIGVRISNGITSHGLAFNVDPDLMYFKHIVPCGIVDKDVTS 188

Query: 136 LREETKEVLPSEK 147
           LR+ETKEVLP E+
Sbjct: 189 LRKETKEVLPGEE 200

BLAST of Chy5G100700 vs. ExPASy TrEMBL
Match: A0A0A0LZZ4 (Lipoyl(octanoyl) transferase OS=Cucumis sativus OX=3659 GN=Csa_1G605760 PE=3 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 6.4e-60
Identity = 120/133 (90.23%), Postives = 125/133 (93.98%), Query Frame = 0

Query: 16  KLGTELHYTERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLERQ--TMACLYGVKA 75
           KLG ELHY+ERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLE+    MACLYGVKA
Sbjct: 69  KLGAELHYSERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLEQTMIEMACLYGVKA 128

Query: 76  RAGEKGETGVWVGDHRKIGAIGVRISNGVTSHGLAFNVDPDLMSFMHIVPCGIVDKDVTS 135
           +AGEKGETGVWVGD RKIGAIGVRISNGVTSHGLAFNVDPDLM F HIVPCGIVDKDVTS
Sbjct: 129 KAGEKGETGVWVGD-RKIGAIGVRISNGVTSHGLAFNVDPDLMYFKHIVPCGIVDKDVTS 188

Query: 136 LREETKEVLPSEK 147
           LR+ETKEVLPSE+
Sbjct: 189 LRKETKEVLPSEE 200

BLAST of Chy5G100700 vs. ExPASy TrEMBL
Match: A0A6J1JLK8 (Lipoyl(octanoyl) transferase OS=Cucurbita maxima OX=3661 GN=LOC111485511 PE=3 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 4.7e-55
Identity = 112/133 (84.21%), Postives = 119/133 (89.47%), Query Frame = 0

Query: 16  KLGTELHYTERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLERQ--TMACLYGVKA 75
           KLG ELHYTERGGDITFHGPNQ+ILYPIISLRD+G GARKYVEKLE+    MA LYGVKA
Sbjct: 69  KLGAELHYTERGGDITFHGPNQAILYPIISLRDIGIGARKYVEKLEQTMIEMAGLYGVKA 128

Query: 76  RAGEKGETGVWVGDHRKIGAIGVRISNGVTSHGLAFNVDPDLMSFMHIVPCGIVDKDVTS 135
           RAGEKGETGVWVGD RKIGAIGVRIS+G+TSHGLAFNVDPDL  F HIVPCGI DKDVTS
Sbjct: 129 RAGEKGETGVWVGD-RKIGAIGVRISSGITSHGLAFNVDPDLSYFKHIVPCGIADKDVTS 188

Query: 136 LREETKEVLPSEK 147
           LR ET EVLPS++
Sbjct: 189 LRRETGEVLPSQE 200

BLAST of Chy5G100700 vs. NCBI nr
Match: TYK26906.1 (octanoyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 237 bits (605), Expect = 1.49e-76
Identity = 120/132 (90.91%), Postives = 123/132 (93.18%), Query Frame = 0

Query: 16  KLGTELHYTERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLERQT--MACLYGVKA 75
           KLG ELHYTERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLE+    MACLYGVKA
Sbjct: 69  KLGAELHYTERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLEQTMIEMACLYGVKA 128

Query: 76  RAGEKGETGVWVGDHRKIGAIGVRISNGVTSHGLAFNVDPDLMSFMHIVPCGIVDKDVTS 135
           RAGEKGETGVWVGD RKIGAIGVRISNG+TSHGLAFNVDPDLM F HIVPCGIVDKDVTS
Sbjct: 129 RAGEKGETGVWVGD-RKIGAIGVRISNGITSHGLAFNVDPDLMYFKHIVPCGIVDKDVTS 188

Query: 136 LREETKEVLPSE 145
           LR+ETKEVLP E
Sbjct: 189 LRKETKEVLPGE 199

BLAST of Chy5G100700 vs. NCBI nr
Match: XP_008451131.1 (PREDICTED: octanoyltransferase [Cucumis melo] >KAA0042285.1 octanoyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 237 bits (605), Expect = 1.49e-76
Identity = 120/132 (90.91%), Postives = 123/132 (93.18%), Query Frame = 0

Query: 16  KLGTELHYTERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLERQT--MACLYGVKA 75
           KLG ELHYTERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLE+    MACLYGVKA
Sbjct: 69  KLGAELHYTERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLEQTMIEMACLYGVKA 128

Query: 76  RAGEKGETGVWVGDHRKIGAIGVRISNGVTSHGLAFNVDPDLMSFMHIVPCGIVDKDVTS 135
           RAGEKGETGVWVGD RKIGAIGVRISNG+TSHGLAFNVDPDLM F HIVPCGIVDKDVTS
Sbjct: 129 RAGEKGETGVWVGD-RKIGAIGVRISNGITSHGLAFNVDPDLMYFKHIVPCGIVDKDVTS 188

Query: 136 LREETKEVLPSE 145
           LR+ETKEVLP E
Sbjct: 189 LRKETKEVLPGE 199

BLAST of Chy5G100700 vs. NCBI nr
Match: KAE8653496.1 (hypothetical protein Csa_007704 [Cucumis sativus])

HSP 1 Score: 236 bits (602), Expect = 1.01e-75
Identity = 119/132 (90.15%), Postives = 124/132 (93.94%), Query Frame = 0

Query: 16  KLGTELHYTERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLERQT--MACLYGVKA 75
           KLG ELHY+ERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLE+    MACLYGVKA
Sbjct: 96  KLGAELHYSERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLEQTMIEMACLYGVKA 155

Query: 76  RAGEKGETGVWVGDHRKIGAIGVRISNGVTSHGLAFNVDPDLMSFMHIVPCGIVDKDVTS 135
           +AGEKGETGVWVGD RKIGAIGVRISNG+TSHGLAFNVDPDLM F HIVPCGIVDKDVTS
Sbjct: 156 KAGEKGETGVWVGD-RKIGAIGVRISNGITSHGLAFNVDPDLMYFKHIVPCGIVDKDVTS 215

Query: 136 LREETKEVLPSE 145
           LR+ETKEVLPSE
Sbjct: 216 LRKETKEVLPSE 226

BLAST of Chy5G100700 vs. NCBI nr
Match: XP_038879976.1 (octanoyltransferase LIP2, mitochondrial [Benincasa hispida] >XP_038879977.1 octanoyltransferase LIP2, mitochondrial [Benincasa hispida])

HSP 1 Score: 229 bits (585), Expect = 1.44e-73
Identity = 117/132 (88.64%), Postives = 120/132 (90.91%), Query Frame = 0

Query: 16  KLGTELHYTERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLERQT--MACLYGVKA 75
           KLG ELHYTERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLE     MACLYGVKA
Sbjct: 69  KLGAELHYTERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLELTMIEMACLYGVKA 128

Query: 76  RAGEKGETGVWVGDHRKIGAIGVRISNGVTSHGLAFNVDPDLMSFMHIVPCGIVDKDVTS 135
           RAGEKGETGVWVGD RKIGAIGVRISNG+TSHGLAFNVDPDL  F HIVPCGI DKDVTS
Sbjct: 129 RAGEKGETGVWVGD-RKIGAIGVRISNGITSHGLAFNVDPDLRYFKHIVPCGIADKDVTS 188

Query: 136 LREETKEVLPSE 145
           LR+E+ EVLPSE
Sbjct: 189 LRKESVEVLPSE 199

BLAST of Chy5G100700 vs. NCBI nr
Match: XP_031736684.1 (LOW QUALITY PROTEIN: octanoyltransferase LIP2, mitochondrial [Cucumis sativus])

HSP 1 Score: 223 bits (567), Expect = 2.01e-70
Identity = 118/132 (89.39%), Postives = 120/132 (90.91%), Query Frame = 0

Query: 16  KLGTELHYTERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLERQTMACLYGVKARA 75
           KLGTELHYTERGGDI F GPNQSILYPIISLRDVGFG RKYVEKLERQTMACLYGVKARA
Sbjct: 82  KLGTELHYTERGGDIAFDGPNQSILYPIISLRDVGFGDRKYVEKLERQTMACLYGVKARA 141

Query: 76  GEKGETGVWVGDHRKIGAIGVRISNGVTSHGLAFNVDPDLMSFM-HIVPC-GIVDKDVTS 135
           GEKGETGVWVGD RKIGAIGVRISNGVTSHGLAFNVDPDLMSF+ HIVP      KDVT 
Sbjct: 142 GEKGETGVWVGD-RKIGAIGVRISNGVTSHGLAFNVDPDLMSFIKHIVPLWDCXYKDVTX 201

Query: 136 LREETKEVLPSE 145
           LR+ETKEVLPSE
Sbjct: 202 LRKETKEVLPSE 212

BLAST of Chy5G100700 vs. TAIR 10
Match: AT1G04640.1 (lipoyltransferase 2 )

HSP 1 Score: 204.1 bits (518), Expect = 7.5e-53
Identity = 100/136 (73.53%), Postives = 114/136 (83.82%), Query Frame = 0

Query: 13  EKPKLGTELHYTERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLERQTM--ACLYG 72
           E  K+G ELHYT+RGGDITFHGP+Q+ILYPIISLR +GFGAR YVE LER  +  A +YG
Sbjct: 66  ELTKIGAELHYTQRGGDITFHGPHQAILYPIISLRSIGFGARNYVETLERSMIEFASIYG 125

Query: 73  VKARAGEKGETGVWVGDHRKIGAIGVRISNGVTSHGLAFNVDPDLMSFMHIVPCGIVDKD 132
           VKARAG K ETGVWVGD RKIGAIGVRIS+G+TSHGLA N+DPD+  F HIVPCGI DK+
Sbjct: 126 VKARAGNKCETGVWVGD-RKIGAIGVRISSGITSHGLALNIDPDMKYFEHIVPCGIADKE 185

Query: 133 VTSLREETKEVLPSEK 147
           VTSLR ET  +LPSE+
Sbjct: 186 VTSLRRETDTLLPSEE 200

BLAST of Chy5G100700 vs. TAIR 10
Match: AT1G04640.2 (lipoyltransferase 2 )

HSP 1 Score: 204.1 bits (518), Expect = 7.5e-53
Identity = 100/136 (73.53%), Postives = 114/136 (83.82%), Query Frame = 0

Query: 13  EKPKLGTELHYTERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLERQTM--ACLYG 72
           E  K+G ELHYT+RGGDITFHGP+Q+ILYPIISLR +GFGAR YVE LER  +  A +YG
Sbjct: 66  ELTKIGAELHYTQRGGDITFHGPHQAILYPIISLRSIGFGARNYVETLERSMIEFASIYG 125

Query: 73  VKARAGEKGETGVWVGDHRKIGAIGVRISNGVTSHGLAFNVDPDLMSFMHIVPCGIVDKD 132
           VKARAG K ETGVWVGD RKIGAIGVRIS+G+TSHGLA N+DPD+  F HIVPCGI DK+
Sbjct: 126 VKARAGNKCETGVWVGD-RKIGAIGVRISSGITSHGLALNIDPDMKYFEHIVPCGIADKE 185

Query: 133 VTSLREETKEVLPSEK 147
           VTSLR ET  +LPSE+
Sbjct: 186 VTSLRRETDTLLPSEE 200

BLAST of Chy5G100700 vs. TAIR 10
Match: AT4G31050.1 (Biotin/lipoate A/B protein ligase family )

HSP 1 Score: 103.6 bits (257), Expect = 1.4e-22
Identity = 52/118 (44.07%), Postives = 78/118 (66.10%), Query Frame = 0

Query: 21  LHYTERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLER---QTMACLYGVKARAGE 80
           ++ TERGG++T+HGP Q ++YPII+LR+       Y+  LE    + ++  + +KA   +
Sbjct: 116 VYRTERGGEVTYHGPGQLVMYPIINLRNHEMDLHWYLRMLEEIVIRVLSSTFSIKASRLD 175

Query: 81  KGETGVWVGDHRKIGAIGVRISNGVTSHGLAFNVDPDLMSFMHIVPCGIVDKDVTSLR 136
            G TGVWVG ++K+ AIG+R+S  +T HGLA NV  DL  F  IVPCGI D+ V +++
Sbjct: 176 -GLTGVWVG-NQKVAAIGIRVSKWITYHGLALNVTTDLTPFNWIVPCGIRDRKVGNIK 231

BLAST of Chy5G100700 vs. TAIR 10
Match: AT1G47578.1 (Biotin/lipoate A/B protein ligase family )

HSP 1 Score: 103.2 bits (256), Expect = 1.8e-22
Identity = 53/118 (44.92%), Postives = 76/118 (64.41%), Query Frame = 0

Query: 20  ELHYTERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLERQTMACLYGVKARAGEK- 79
           +++ TERGG++T+HGP Q ++YPII+LR+       Y+ KLE   +  L    A    + 
Sbjct: 117 DVYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRKLEEVVIRVLSSAFAINASRL 176

Query: 80  -GETGVWVGDHRKIGAIGVRISNGVTSHGLAFNVDPDLMSFMHIVPCGIVDKDVTSLR 136
            G TGVWVG ++K+ AIG+R+S  +T HGLA NV  DL  F  IVPCGI ++ V S++
Sbjct: 177 DGFTGVWVG-NKKMAAIGIRVSKWMTYHGLALNVTTDLTPFNSIVPCGIRNRGVGSVK 233

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SXP71.0e-5173.53Octanoyltransferase LIP2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=LIP2 ... [more]
Q5LDM35.8e-2651.18Octanoyltransferase OS=Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM ... [more]
Q64UP85.8e-2651.18Octanoyltransferase OS=Bacteroides fragilis (strain YCH46) OX=295405 GN=lipB PE=... [more]
Q8A8S82.2e-2547.73Octanoyltransferase OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 207... [more]
B8HGZ82.4e-2444.68Octanoyltransferase OS=Pseudarthrobacter chlorophenolicus (strain ATCC 700700 / ... [more]
Match NameE-valueIdentityDescription
A0A5A7TLG43.7e-6090.23Lipoyl(octanoyl) transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca... [more]
A0A5D3DTA63.7e-6090.23Lipoyl(octanoyl) transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3BQW03.7e-6090.23Lipoyl(octanoyl) transferase OS=Cucumis melo OX=3656 GN=LOC103492510 PE=3 SV=1[more]
A0A0A0LZZ46.4e-6090.23Lipoyl(octanoyl) transferase OS=Cucumis sativus OX=3659 GN=Csa_1G605760 PE=3 SV=... [more]
A0A6J1JLK84.7e-5584.21Lipoyl(octanoyl) transferase OS=Cucurbita maxima OX=3661 GN=LOC111485511 PE=3 SV... [more]
Match NameE-valueIdentityDescription
TYK26906.11.49e-7690.91octanoyltransferase [Cucumis melo var. makuwa][more]
XP_008451131.11.49e-7690.91PREDICTED: octanoyltransferase [Cucumis melo] >KAA0042285.1 octanoyltransferase ... [more]
KAE8653496.11.01e-7590.15hypothetical protein Csa_007704 [Cucumis sativus][more]
XP_038879976.11.44e-7388.64octanoyltransferase LIP2, mitochondrial [Benincasa hispida] >XP_038879977.1 octa... [more]
XP_031736684.12.01e-7089.39LOW QUALITY PROTEIN: octanoyltransferase LIP2, mitochondrial [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT1G04640.17.5e-5373.53lipoyltransferase 2 [more]
AT1G04640.27.5e-5373.53lipoyltransferase 2 [more]
AT4G31050.11.4e-2244.07Biotin/lipoate A/B protein ligase family [more]
AT1G47578.11.8e-2244.92Biotin/lipoate A/B protein ligase family [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000544OctanoyltransferaseTIGRFAMTIGR00214TIGR00214coord: 19..138
e-value: 7.9E-34
score: 114.8
IPR000544OctanoyltransferasePIRSFPIRSF016262LPLasecoord: 3..145
e-value: 7.5E-44
score: 147.6
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 3..146
e-value: 7.2E-47
score: 161.3
NoneNo IPR availablePANTHERPTHR10993OCTANOYLTRANSFERASEcoord: 14..139
NoneNo IPR availablePANTHERPTHR10993:SF7LIPOYLTRANSFERASE 2, MITOCHONDRIAL-RELATEDcoord: 14..139
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 10..140
IPR004143Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL), catalytic domainPFAMPF03099BPL_LplA_LipBcoord: 12..114
e-value: 3.2E-8
score: 33.5
IPR004143Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL), catalytic domainPROSITEPS51733BPL_LPL_CATALYTICcoord: 1..146
score: 39.306755

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy5G100700.1Chy5G100700.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009249 protein lipoylation
biological_process GO:0006464 cellular protein modification process
molecular_function GO:0033819 lipoyl(octanoyl) transferase activity
molecular_function GO:0102555 octanoyl transferase activity (acting on glycine-cleavage complex H protein)