Chy5G100290 (gene) Cucumber (hystrix) v1

Overview
NameChy5G100290
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionTranslation initiation factor IF-3
LocationchrH05: 14529639 .. 14532519 (+)
RNA-Seq ExpressionChy5G100290
SyntenyChy5G100290
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGGGCTCTCCGGCAGTTTCCCTTTCAAATCTCTCTTATCCCAATCCTCCGCCGCTGCCACCACCACCACCTCCTCCGCCCCACCTGCCGCTACCTCCTCCTCTTCCACTACTCCCTTCCGAACCTCATTCCCCTCTCTTCCCATCTCCTCCTCTAATTCCAATGCCTTCAAATTCCGCCTTCACAAATCCAACTCTCTACGTCTCCTCTTTTCCTCTCCTATTCGTACTTCTGTATTCGCTCGACTCGGCGGCGCTTCTCGATCTTCCGGACCGCCTGATTCTAAGCGCTCTAACGATGAAATTGGTCTCGATATTTCCGCCATCAAGTTTGTGTCCTGTACTTGTACTTGTATGATTTATGGATTTTTTTTTTTTTTTTTTTTTTTAATTTGGAGTTCTTATTTGTTTGGTTGCGTTTTTTGTGATTTATTTGTATAGGTCAACTAATGTGAGGCTTATCGATGCTGCTCAGAATATGGTAGGTGTTTAAGTTTTTGGATGGTTTGTGCTTTGGGAAACCATATATGTGTGTGTGTGTGTGTGTTTGTCTGTTTCCTTTTGCTACAATGCAATACCTGTATGCTAGGTGTGGTAGTTTGAAACGTTCATGTTCTTATAATGAATAGGTTGGAGTTGTGTCGAAGGCCCAAGCCATTCAAATGGCTGAAGACGCTGAACTTGATTTGGTATGTCTTCTTGCATTGTTTGTTGTTAAAATCATTGTTTGTCTATGAATTCCAAAGAACCTTGCACCAAACTTGCATCGTTTGTTGTTAAAATCATTGTTTGTCTATGAATTCCAAAGAACCTTGCACCAAACTTTAATCTCCTTTTGAGGAAACATTGTATAGTTCATATTTCTTTTACTAAAAATGGAATTAAAAGCATATAATAATAAACTATTCGAAAAATTATAGATATAAGTTATAGCCTTTCATCCATAGCTAGAAGAACCATCGCATAGTGAATGGATTTTATTTCACAGCTGTGCATTAGGCCAATTTTACTGTTTGCTCTGATATAAAATTGTAATTTCCTTCCTTATGCCATAAACTTGCAAAGAAATCCTATTTGTCAACACGAAACTTGGATAAATGCTGGATAATGCCTTGTTATTAAGATATATGTTAAAAATTTCTTAGAACCTTTGAGTTGTCTGGATGCTCCTGGTGCAATAAACGATGCTCATTTCACATTCTGTTTTTTACTTCTTAACTTGGATAAAGAAGGTCGGGGGACTAATATTTTTCCTGGAATCCTAATATTTTCGTTGCTTTATGTAAGGTTTAGCTAATTCTTTTTATTCCGTTCAGGTTGTAGTATCTCCTGATGCAGATCCTCCAGTTGTTAGAATAATGGATTACAAGTATGCCACTTTCTCTCTCTGGTTTCCCAATTCCCTCTATACTTGTGGATAAGGTTGTATTTTGCTCGAGTTCAATGCCAATGTGATCAACTTTTATGATTAATTTCAATTCTTATGTTAAACATGTTGTGATGATATATTTTTAAATTAGTACTTTACTTTTAATTGAATGTTTCCGAAAAAGTATGGAAATATGGTGCAGTATAACGGTGACATAATGAAATAGGATGATTGTTATGTTGTTTTCACTGGGAGAATTGGAAGGCCACTTTTTTTTTTTTTCCTTACTACTATTTTGTTACTTTTATGTTGAGAATTTTCTTTTCCCGTCTTTTGTTTCTCAGTAAATATAGGTATGAACTGCAAAAAAAGAAAAGAGGACAACAGAAGAAAAGTGCTGGTGAATCTCTTTTTTAATAGAGTCCGCATATTTTCTTTGGTACAAAAATCTTTCAGATTTGTGGAAAGATACTGACAAAGTGTGGTTTTGAATGTTAGCAACTCGCATGGATTTGAAAGAGCTTAAAATGGGGTAACACATTCCTTCATCTCTCTGTCTCTTTTCTCTCTGGAGGTTTCCTTTTTTTTTTCTTCTTTTTCTCGACTTAAAGCCACTTGAACTTGTGGCGTCTAAGATCAATAATCACCCAGCCTGCAAAGCAAAGAGACATGAGAGGCCTTTGTCTGCTCTTTCAGAAACATTTATGCACATGTTCCTTTTCTCAATCTCGACCTTCTGATTGTTATTATTGTTGCACAGTTATAACATCGACCAACATGATTACTCTGTCCGTTTGAAAGCTGCAAAGAAATTTTTAAAAGACGGAGACAAGGTTTCTTATCAAATCTTCTATATCTTTCATCTTCTTTTCATGCATCCAGATATATCGTTATAATTTGTCTGAATCACTTTGGAAATCTTTTATCGAATATTGTAGGTTAAAGTTATAGTGAACCTGAAAGGTCGTGAAAATGAGTTCAGAAACATAGCAATTGAGCTCATCAGACGTTTCCAGGAAGAAGTTGGGGAGGTATTCATGTTCTATCAATTTTGTCTCGACTTCAATATCTGTTTATCATGGCAATACTAGAGAAAATGATTGCCCATTGCCCCTTATTGTGGTCTTGTTACTCAATACTACAAATAATGGTTGTTTTATAGAACCATTTTCTTAAAAGAATGCTTAGTACCTAAAACATTGTGATCATATTGCTTGTTTGTCTTGTTTTTTTCTTATTTGTGTCTGCTAATCTCTCTTTATCCCATATCCGTGCTTCGGGTTAATACCTGAAACCAACTCGATATACCTTCTCTTTATTTATCTCTCTTCCCCATTGACGTTCTCGATATTTTATTATACACAGCTTGCAACTGAAGAGGCCAAAAACTTCAGAGACAGGAATATATTTATTGTTCTAGTCCCTAACAAGGCTGTTCTTCAAAAGGCCCAAGAACCTCCCAAGAAAAAAGATAAACCTGCAGTTAATGAAGTTTCTGCCGGTGTTTAA

mRNA sequence

ATGGCTGGGCTCTCCGGCAGTTTCCCTTTCAAATCTCTCTTATCCCAATCCTCCGCCGCTGCCACCACCACCACCTCCTCCGCCCCACCTGCCGCTACCTCCTCCTCTTCCACTACTCCCTTCCGAACCTCATTCCCCTCTCTTCCCATCTCCTCCTCTAATTCCAATGCCTTCAAATTCCGCCTTCACAAATCCAACTCTCTACGTCTCCTCTTTTCCTCTCCTATTCGTACTTCTGTATTCGCTCGACTCGGCGGCGCTTCTCGATCTTCCGGACCGCCTGATTCTAAGCGCTCTAACGATGAAATTGGTCTCGATATTTCCGCCATCAAGTCAACTAATGTGAGGCTTATCGATGCTGCTCAGAATATGGTTGGAGTTGTGTCGAAGGCCCAAGCCATTCAAATGGCTGAAGACGCTGAACTTGATTTGGTTGTAGTATCTCCTGATGCAGATCCTCCAGTTGTTAGAATAATGGATTACAATAAATATAGGTATGAACTGCAAAAAAAGAAAAGAGGACAACAGAAGAAAAGTGCTGCAACTCGCATGGATTTGAAAGAGCTTAAAATGGGTTATAACATCGACCAACATGATTACTCTGTCCGTTTGAAAGCTGCAAAGAAATTTTTAAAAGACGGAGACAAGGTTAAAGTTATAGTGAACCTGAAAGGTCGTGAAAATGAGTTCAGAAACATAGCAATTGAGCTCATCAGACGTTTCCAGGAAGAAGTTGGGGAGCTTGCAACTGAAGAGGCCAAAAACTTCAGAGACAGGAATATATTTATTGTTCTAGTCCCTAACAAGGCTGTTCTTCAAAAGGCCCAAGAACCTCCCAAGAAAAAAGATAAACCTGCAGTTAATGAAGTTTCTGCCGGTGTTTAA

Coding sequence (CDS)

ATGGCTGGGCTCTCCGGCAGTTTCCCTTTCAAATCTCTCTTATCCCAATCCTCCGCCGCTGCCACCACCACCACCTCCTCCGCCCCACCTGCCGCTACCTCCTCCTCTTCCACTACTCCCTTCCGAACCTCATTCCCCTCTCTTCCCATCTCCTCCTCTAATTCCAATGCCTTCAAATTCCGCCTTCACAAATCCAACTCTCTACGTCTCCTCTTTTCCTCTCCTATTCGTACTTCTGTATTCGCTCGACTCGGCGGCGCTTCTCGATCTTCCGGACCGCCTGATTCTAAGCGCTCTAACGATGAAATTGGTCTCGATATTTCCGCCATCAAGTCAACTAATGTGAGGCTTATCGATGCTGCTCAGAATATGGTTGGAGTTGTGTCGAAGGCCCAAGCCATTCAAATGGCTGAAGACGCTGAACTTGATTTGGTTGTAGTATCTCCTGATGCAGATCCTCCAGTTGTTAGAATAATGGATTACAATAAATATAGGTATGAACTGCAAAAAAAGAAAAGAGGACAACAGAAGAAAAGTGCTGCAACTCGCATGGATTTGAAAGAGCTTAAAATGGGTTATAACATCGACCAACATGATTACTCTGTCCGTTTGAAAGCTGCAAAGAAATTTTTAAAAGACGGAGACAAGGTTAAAGTTATAGTGAACCTGAAAGGTCGTGAAAATGAGTTCAGAAACATAGCAATTGAGCTCATCAGACGTTTCCAGGAAGAAGTTGGGGAGCTTGCAACTGAAGAGGCCAAAAACTTCAGAGACAGGAATATATTTATTGTTCTAGTCCCTAACAAGGCTGTTCTTCAAAAGGCCCAAGAACCTCCCAAGAAAAAAGATAAACCTGCAGTTAATGAAGTTTCTGCCGGTGTTTAA

Protein sequence

MAGLSGSFPFKSLLSQSSAAATTTTSSAPPAATSSSSTTPFRTSFPSLPISSSNSNAFKFRLHKSNSLRLLFSSPIRTSVFARLGGASRSSGPPDSKRSNDEIGLDISAIKSTNVRLIDAAQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQQKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAIELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKPAVNEVSAGV*
Homology
BLAST of Chy5G100290 vs. ExPASy Swiss-Prot
Match: Q94B52 (Translation initiation factor IF3-4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IF3-4 PE=2 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 8.5e-77
Identity = 154/211 (72.99%), Postives = 181/211 (85.78%), Query Frame = 0

Query: 85  GGASRSSGP--PDSKRSNDEIGLDISAIKSTNVRLIDAAQNMVGVVSKAQAIQMAEDAEL 144
           GG SR  G      K S D+  LDISAI+S  VRLID  QNM+G+VSK +A++ AEDAEL
Sbjct: 64  GGGSRFPGDRRGRQKESEDDDSLDISAIRSATVRLIDDQQNMIGLVSKEEAVRRAEDAEL 123

Query: 145 DLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQQKKSAATRMDLKELKMGYNIDQHDYSV 204
           DLV++SPDADPPVVR+MDY+KYRYE QK+K+ QQKK+  TRMDLKELKMGYNIDQHDYSV
Sbjct: 124 DLVILSPDADPPVVRMMDYSKYRYEQQKRKKEQQKKT--TRMDLKELKMGYNIDQHDYSV 183

Query: 205 RLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAIELIRRFQEEVGELATEEAKNFRDRNIF 264
           R++AA+KFL+DGDKVKVIVN+KGRENEFRNIAIEL+RRFQ E+GEL TEE+KNFRDRN+F
Sbjct: 184 RMRAARKFLQDGDKVKVIVNMKGRENEFRNIAIELLRRFQTEIGELGTEESKNFRDRNLF 243

Query: 265 IVLVPNKAVLQKAQE-PPKKKDKPAVNEVSA 293
           IVLVPNK V++K QE PPKKK KPA ++VSA
Sbjct: 244 IVLVPNKEVIRKVQEPPPKKKKKPADDKVSA 272

BLAST of Chy5G100290 vs. ExPASy Swiss-Prot
Match: O82234 (Translation initiation factor IF3-2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IF3-2 PE=2 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 7.5e-73
Identity = 165/274 (60.22%), Postives = 203/274 (74.09%), Query Frame = 0

Query: 35  SSSTTPFRTSFPSLPISSSNSNAF-------KFRLHKSN-SLRLLFSSPIR----TSVFA 94
           +SST  F   F  +  + S+ + F         RL K+  S   L  SP R    T  F 
Sbjct: 5   TSSTVGFNAVFTGITKTVSSHSLFSVDSKLCSLRLSKTELSFTNLTPSPRRAFAVTCRFG 64

Query: 95  RLGGASRSSGPPDSKRSND---EIGLDISAIKSTNVRLIDAAQNMVGVVSKAQAIQMAED 154
             GG  R SG     R  +   +  LDIS+I+S  VRLID  QNM+G+VSK +A++MA+D
Sbjct: 65  GGGGGYRFSGDNRRGRPKEAEIDEALDISSIRSATVRLIDGQQNMLGLVSKDEAVRMADD 124

Query: 155 AELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQQKKSAATRMDLKELKMGYNIDQHD 214
           AELDLV++SPDADPPVV++MDY+KYRYE QK+K+ QQKK+  TRMDLKELKMGYNIDQHD
Sbjct: 125 AELDLVILSPDADPPVVKMMDYSKYRYEQQKRKKDQQKKT--TRMDLKELKMGYNIDQHD 184

Query: 215 YSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAIELIRRFQEEVGELATEEAKNFRDR 274
           YSVRL+AA+KFL+DGDKVKVIV++KGRENEFRNIAIEL+RRFQ E+GELATEE+KNFRDR
Sbjct: 185 YSVRLRAAQKFLQDGDKVKVIVSMKGRENEFRNIAIELLRRFQTEIGELATEESKNFRDR 244

Query: 275 NIFIVLVPNKAVLQKAQEPP-KKKDKPAVNEVSA 293
           N+FI+LVPNK +++K QEPP +KK K A NE SA
Sbjct: 245 NMFIILVPNKEMIRKPQEPPTRKKKKTAENEASA 276

BLAST of Chy5G100290 vs. ExPASy Swiss-Prot
Match: B0JKQ1 (Translation initiation factor IF-3 OS=Microcystis aeruginosa (strain NIES-843) OX=449447 GN=infC PE=3 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.2e-30
Identity = 72/176 (40.91%), Postives = 117/176 (66.48%), Query Frame = 0

Query: 95  DSKRSNDEIGLDISAIKSTNVRLIDAAQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPP 154
           D +R+  ++      I+   +R+ID+  + +G+++ A+A+++AE+ ELDLV+VS  A PP
Sbjct: 3   DKRRTTRDLPQINERIRFPEIRVIDSDGSQLGIITPAEALRVAEEKELDLVLVSETASPP 62

Query: 155 VVRIMDYNKYRYELQKKKRGQQKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDG 214
           V RIMDY KY++E +KK R  +KK      D+KE+KM Y ID+HDY+VR+  A++FLK G
Sbjct: 63  VCRIMDYGKYKFEQEKKAREAKKKQHTA--DIKEVKMRYKIDEHDYNVRVNQAQRFLKAG 122

Query: 215 DKVKVIVNLKGRENEFRNIAIELIRRFQEEVGELA-TEEAKNFRDRNIFIVLVPNK 270
           DKVK  +  +GRE +  N+A EL+ R  +++ ++A  ++A     RN+ ++L P K
Sbjct: 123 DKVKATITFRGREIQHSNLAEELLARMAKDLQDVAEVQQAPKKEGRNMMMMLSPKK 176

BLAST of Chy5G100290 vs. ExPASy Swiss-Prot
Match: Q8R9C2 (Translation initiation factor IF-3 OS=Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) OX=273068 GN=infC PE=3 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 7.8e-30
Identity = 69/161 (42.86%), Postives = 110/161 (68.32%), Query Frame = 0

Query: 110 IKSTNVRLIDAAQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPPVVRIMDYNKYRYELQ 169
           I+   VRLID     +G++S  +A ++A++  LDLV + P+A+PPV ++MD+ KYRYEL 
Sbjct: 21  IRDKEVRLIDQDGKQIGIMSAKEAYKIAQERHLDLVKIVPNANPPVCKLMDFGKYRYELS 80

Query: 170 KKKRGQQKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENE 229
           K+++  +KK     +++KE++M  NI+ HD+ V+LK+A KFLKDGDKVKV +  +GRE  
Sbjct: 81  KREKEAKKKQKI--INVKEIRMSPNIEDHDFGVKLKSAIKFLKDGDKVKVTIRFRGREAA 140

Query: 230 FRNIAIELIRRFQEEVGELA-TEEAKNFRDRNIFIVLVPNK 270
             ++A +L++RF EE+ E    E+A +   RN+ +V+ P K
Sbjct: 141 HTSLAEDLLKRFAEELREYGNVEKAPSMDGRNMMMVIAPKK 179

BLAST of Chy5G100290 vs. ExPASy Swiss-Prot
Match: Q0IDZ5 (Translation initiation factor IF-3 OS=Synechococcus sp. (strain CC9311) OX=64471 GN=infC PE=3 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 7.8e-30
Identity = 80/181 (44.20%), Postives = 116/181 (64.09%), Query Frame = 0

Query: 110 IKSTNVRLIDAAQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPPVVRIMDYNKYRYELQ 169
           I    +R++DA    +GV+ + QA+++A + ELDLV+VS  ADPPV RIMDY K+++E +
Sbjct: 23  INYPQLRVVDADGEQLGVIDREQALEVARERELDLVLVSEKADPPVCRIMDYGKFKFEQE 82

Query: 170 KKKRGQQKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENE 229
           KK +  +KKS  T  ++KE+KM Y IDQHDY VR+  A++FLK GDKVK  V  +GRE +
Sbjct: 83  KKAKEAKKKSHQT--EVKEVKMRYKIDQHDYDVRIGQAQRFLKAGDKVKCTVIFRGREIQ 142

Query: 230 FRNIAIELIRRFQEEVGELATEEAKNFRD-RNIFIVLVPNKAVLQKAQEPPKKKDKPAVN 289
              +A  L+RR  +++ E A  +    R+ RN+ + L P KA L K     K KD+  VN
Sbjct: 143 HTALAEVLLRRMAKDLEEPAEVQQPPKREGRNMIMFLTPRKAPLVK-----KDKDEEVVN 196

BLAST of Chy5G100290 vs. ExPASy TrEMBL
Match: A0A0A0LH34 (Translation initiation factor IF-3 OS=Cucumis sativus OX=3659 GN=Csa_2G036070 PE=3 SV=1)

HSP 1 Score: 506.5 bits (1303), Expect = 7.4e-140
Identity = 285/299 (95.32%), Postives = 286/299 (95.65%), Query Frame = 0

Query: 1   MAGLSGSFPFKSLLSQSSAAATTT-TSSAPPAATSSSSTTPFRTSFPSLPISSSNSNAFK 60
           MAGLSGSFPFKSLLSQSSAAATTT +SSA P  TSSSSTTPFRTSFPSLPISSSNSNAFK
Sbjct: 1   MAGLSGSFPFKSLLSQSSAAATTTSSSSAAPPPTSSSSTTPFRTSFPSLPISSSNSNAFK 60

Query: 61  FRLHKSNSLRLLF----SSPIRTSVFARLGGASRSSGPPDSKRSNDEIGLDISAIKSTNV 120
           FRLHKSNSLRLLF    SSPIRTSVFARLGGA RSSGPPDSKRSNDEIGLDISAIKS NV
Sbjct: 61  FRLHKSNSLRLLFSSPVSSPIRTSVFARLGGAPRSSGPPDSKRSNDEIGLDISAIKSINV 120

Query: 121 RLIDAAQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQ 180
           RLIDA QNMVGVVSKAQAIQMAEDAELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQ
Sbjct: 121 RLIDAEQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQ 180

Query: 181 QKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAI 240
           QKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAI
Sbjct: 181 QKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAI 240

Query: 241 ELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKPAVNEVSAGV 295
           ELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKPAVN VSA V
Sbjct: 241 ELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKPAVNGVSASV 299

BLAST of Chy5G100290 vs. ExPASy TrEMBL
Match: A0A1S3BV82 (Translation initiation factor IF-3 OS=Cucumis melo OX=3656 GN=LOC103493630 PE=3 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 6.5e-136
Identity = 277/299 (92.64%), Postives = 284/299 (94.98%), Query Frame = 0

Query: 1   MAGLSGSFPFKSLLSQSSAAATTTTSSAPPAATSSSSTTPFRTSFPSLPISSS-NSNAFK 60
           MAGLSGSFPFKSLLSQSSAA+TTT+ +A P  TSSSSTTPFRTS PSLPISSS NSNAFK
Sbjct: 1   MAGLSGSFPFKSLLSQSSAASTTTSPAAAP--TSSSSTTPFRTSTPSLPISSSFNSNAFK 60

Query: 61  FRLHKSNSLRLLF----SSPIRTSVFARLGGASRSSGPPDSKRSNDEIGLDISAIKSTNV 120
            RLHKSNSLRL+F    SSPIRTSVFARLGGA RSSGPPDSKRSNDE GLD+SAI+S NV
Sbjct: 61  LRLHKSNSLRLVFSFPVSSPIRTSVFARLGGAPRSSGPPDSKRSNDEFGLDVSAIRSNNV 120

Query: 121 RLIDAAQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQ 180
           RLIDA QNMVGVVSKAQAIQMAEDAELDLVV+SPDADPPVVRIMDYNKYRYELQKKKRGQ
Sbjct: 121 RLIDAQQNMVGVVSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQKKKRGQ 180

Query: 181 QKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAI 240
           QKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAI
Sbjct: 181 QKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAI 240

Query: 241 ELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKPAVNEVSAGV 295
           ELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKPAVNEVSAGV
Sbjct: 241 ELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKPAVNEVSAGV 297

BLAST of Chy5G100290 vs. ExPASy TrEMBL
Match: A0A6J1DYM5 (Translation initiation factor IF-3 OS=Momordica charantia OX=3673 GN=LOC111024717 PE=3 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 3.3e-124
Identity = 247/294 (84.01%), Postives = 269/294 (91.50%), Query Frame = 0

Query: 1   MAGLSGSFPFKSLLSQSSAAATTTTSSAPPAATSSSSTTPFRTSFPSLPISSSNSNAFKF 60
           MAGL+  FPFK LLSQSSA   TT+S+A  +++S+++  PFRTS   LP+SSS SNAF F
Sbjct: 1   MAGLTSRFPFKPLLSQSSA---TTSSAAASSSSSAAAAPPFRTSISPLPLSSSESNAFGF 60

Query: 61  RLHKSNSLRLLFSSPIRTSVFARLGGASRSSGPPDSKRSNDEIGLDISAIKSTNVRLIDA 120
           RLH SNSLRL+ SSPIR SVFAR GGA RSSGP DSKRS+D+ GLD+SAI+S NVRLIDA
Sbjct: 61  RLHNSNSLRLIVSSPIRRSVFARFGGAPRSSGPADSKRSDDDFGLDVSAIRSNNVRLIDA 120

Query: 121 AQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQQKKSA 180
            QNMVG+VSKAQAIQMAEDAELDLVV+SPDADPPVVRIMDYNKYRYELQKKKRGQQKKSA
Sbjct: 121 QQNMVGIVSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQKKKRGQQKKSA 180

Query: 181 ATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAIELIRR 240
           ATRMDLKELKMGYNIDQHDYSVRLKAA++FLKDGDKVKVIVNLKGRENEFRNIAIELIRR
Sbjct: 181 ATRMDLKELKMGYNIDQHDYSVRLKAARRFLKDGDKVKVIVNLKGRENEFRNIAIELIRR 240

Query: 241 FQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKPAVNEVSAGV 295
           FQ+E+GELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKD PA+NEVSAGV
Sbjct: 241 FQDEIGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDNPAINEVSAGV 291

BLAST of Chy5G100290 vs. ExPASy TrEMBL
Match: A0A6J1KDY9 (Translation initiation factor IF-3 OS=Cucurbita maxima OX=3661 GN=LOC111493999 PE=3 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 7.4e-116
Identity = 239/295 (81.02%), Postives = 258/295 (87.46%), Query Frame = 0

Query: 1   MAGLSGSFPFKSLLSQSSAAATTTTSSAPPAATSSSSTTPFRTSFPSLPISSSNSNAFKF 60
           MAGLSG FPFKS+ SQSSA AT TTS       SSSS TPFRTS   LP  SS SNA +F
Sbjct: 1   MAGLSGRFPFKSISSQSSATATATTS-------SSSSATPFRTSIFPLPFPSSKSNAVRF 60

Query: 61  RLHKSNSLRLLFSSPIRTSVFARLGGASRSSGPPDSKRSNDEIGLDISAIKSTNVRLIDA 120
           RLH SNSLRL+ SSPIRTSVFAR GGA RSSGP  SKRS D + LD+S+I+S NVRLID 
Sbjct: 61  RLHSSNSLRLV-SSPIRTSVFARYGGAPRSSGPTGSKRSEDSLALDVSSIRSNNVRLIDE 120

Query: 121 AQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQQKKSA 180
            QNMVG+V K+QAIQMAEDAELDLVV+SPDADPPVVRIMDY+KYRY+LQKKKR QQKKSA
Sbjct: 121 QQNMVGIVPKSQAIQMAEDAELDLVVLSPDADPPVVRIMDYSKYRYDLQKKKREQQKKSA 180

Query: 181 ATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAIELIRR 240
           ATRMDLKELKMGY IDQHDYSVRL+AAKKFL+DGDKVKVIVNLKGRENEFR IAIEL++R
Sbjct: 181 ATRMDLKELKMGYKIDQHDYSVRLRAAKKFLQDGDKVKVIVNLKGRENEFRKIAIELVKR 240

Query: 241 FQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKP-AVNEVSAGV 295
           FQEE+GELATEEAKNFRDRN+FIVL PNKAVLQK+QEPPKKKDKP AVNEVSAGV
Sbjct: 241 FQEELGELATEEAKNFRDRNVFIVLAPNKAVLQKSQEPPKKKDKPAAVNEVSAGV 287

BLAST of Chy5G100290 vs. ExPASy TrEMBL
Match: A0A6J1G3Y5 (Translation initiation factor IF-3 OS=Cucurbita moschata OX=3662 GN=LOC111450575 PE=3 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 9.7e-116
Identity = 238/295 (80.68%), Postives = 261/295 (88.47%), Query Frame = 0

Query: 1   MAGLSGSFPFKSLLSQSSAAATTTTSSAPPAATSSSSTTPFRTSFPSLPISSSNSNAFKF 60
           MAGLSG FPFKS+ SQSSA AT TT++     +SSSS TPFRTS   LP+ SS SNAF+F
Sbjct: 1   MAGLSGRFPFKSISSQSSATATATTTT-----SSSSSATPFRTSIFPLPLPSSKSNAFRF 60

Query: 61  RLHKSNSLRLLFSSPIRTSVFARLGGASRSSGPPDSKRSNDEIGLDISAIKSTNVRLIDA 120
           RLH SNSLRL+ SSPIRTSVFAR GGA RSSGP  SKRS D + LD+S+I+S NVRLID 
Sbjct: 61  RLHNSNSLRLV-SSPIRTSVFARYGGAPRSSGPAGSKRSEDSLALDVSSIRSNNVRLIDE 120

Query: 121 AQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQQKKSA 180
            QNMVG+V K+QAIQMAEDAELDLVV+SPDADPPVVRIMDY+KYRY+LQKKKR QQKKSA
Sbjct: 121 QQNMVGIVPKSQAIQMAEDAELDLVVLSPDADPPVVRIMDYSKYRYDLQKKKREQQKKSA 180

Query: 181 ATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAIELIRR 240
           ATRMDLKELKMGY IDQHDYSVRL+AAKKFL+DGDKVKVIVNLKGRENEFR IAIEL++R
Sbjct: 181 ATRMDLKELKMGYKIDQHDYSVRLRAAKKFLQDGDKVKVIVNLKGRENEFRKIAIELVKR 240

Query: 241 FQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKP-AVNEVSAGV 295
           FQEE+GELATEEAKNFRDRN+FIVL PNKAVLQK+QEPPKKKDKP AVNEVSA V
Sbjct: 241 FQEELGELATEEAKNFRDRNVFIVLAPNKAVLQKSQEPPKKKDKPAAVNEVSARV 289

BLAST of Chy5G100290 vs. NCBI nr
Match: XP_004144726.1 (translation initiation factor IF3-4, chloroplastic [Cucumis sativus] >KGN61058.1 hypothetical protein Csa_021304 [Cucumis sativus])

HSP 1 Score: 507 bits (1305), Expect = 1.30e-179
Identity = 285/299 (95.32%), Postives = 286/299 (95.65%), Query Frame = 0

Query: 1   MAGLSGSFPFKSLLSQSSAAATTTTSS-APPAATSSSSTTPFRTSFPSLPISSSNSNAFK 60
           MAGLSGSFPFKSLLSQSSAAATTT+SS A P  TSSSSTTPFRTSFPSLPISSSNSNAFK
Sbjct: 1   MAGLSGSFPFKSLLSQSSAAATTTSSSSAAPPPTSSSSTTPFRTSFPSLPISSSNSNAFK 60

Query: 61  FRLHKSNSLRLLFSSP----IRTSVFARLGGASRSSGPPDSKRSNDEIGLDISAIKSTNV 120
           FRLHKSNSLRLLFSSP    IRTSVFARLGGA RSSGPPDSKRSNDEIGLDISAIKS NV
Sbjct: 61  FRLHKSNSLRLLFSSPVSSPIRTSVFARLGGAPRSSGPPDSKRSNDEIGLDISAIKSINV 120

Query: 121 RLIDAAQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQ 180
           RLIDA QNMVGVVSKAQAIQMAEDAELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQ
Sbjct: 121 RLIDAEQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQ 180

Query: 181 QKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAI 240
           QKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAI
Sbjct: 181 QKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAI 240

Query: 241 ELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKPAVNEVSAGV 294
           ELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKPAVN VSA V
Sbjct: 241 ELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKPAVNGVSASV 299

BLAST of Chy5G100290 vs. NCBI nr
Match: XP_008452687.1 (PREDICTED: translation initiation factor IF-3 [Cucumis melo])

HSP 1 Score: 494 bits (1271), Expect = 1.83e-174
Identity = 277/299 (92.64%), Postives = 284/299 (94.98%), Query Frame = 0

Query: 1   MAGLSGSFPFKSLLSQSSAAATTTTSSAPPAATSSSSTTPFRTSFPSLPISSS-NSNAFK 60
           MAGLSGSFPFKSLLSQSSAA+TTT+ +A P  TSSSSTTPFRTS PSLPISSS NSNAFK
Sbjct: 1   MAGLSGSFPFKSLLSQSSAASTTTSPAAAP--TSSSSTTPFRTSTPSLPISSSFNSNAFK 60

Query: 61  FRLHKSNSLRLLFS----SPIRTSVFARLGGASRSSGPPDSKRSNDEIGLDISAIKSTNV 120
            RLHKSNSLRL+FS    SPIRTSVFARLGGA RSSGPPDSKRSNDE GLD+SAI+S NV
Sbjct: 61  LRLHKSNSLRLVFSFPVSSPIRTSVFARLGGAPRSSGPPDSKRSNDEFGLDVSAIRSNNV 120

Query: 121 RLIDAAQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQ 180
           RLIDA QNMVGVVSKAQAIQMAEDAELDLVV+SPDADPPVVRIMDYNKYRYELQKKKRGQ
Sbjct: 121 RLIDAQQNMVGVVSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQKKKRGQ 180

Query: 181 QKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAI 240
           QKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAI
Sbjct: 181 QKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAI 240

Query: 241 ELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKPAVNEVSAGV 294
           ELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKPAVNEVSAGV
Sbjct: 241 ELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKPAVNEVSAGV 297

BLAST of Chy5G100290 vs. NCBI nr
Match: XP_038891171.1 (translation initiation factor IF3-4, chloroplastic [Benincasa hispida])

HSP 1 Score: 459 bits (1181), Expect = 8.65e-161
Identity = 260/299 (86.96%), Postives = 274/299 (91.64%), Query Frame = 0

Query: 1   MAGLSGSFPFKSLLSQSSAAATTTTSSAPPAATSSSSTTPFRTSFPSLPISSSNSNAFKF 60
           MAGLSG FPFK LLSQS+ A  TTTS+A  AATSSSS TPFRTS   LP+SSS +NAFKF
Sbjct: 1   MAGLSGRFPFKPLLSQSTTA--TTTSAA--AATSSSSATPFRTSIRPLPLSSSKANAFKF 60

Query: 61  RLHKSNSLRLLFS----SPIRTSVFARLGGASRSSGPPDSKRSNDEIGLDISAIKSTNVR 120
           RL KSNSLRL+ S    SPIRTSVF+R GGA RSSGP DSKRS+DE GLD+SAI+S NVR
Sbjct: 61  RLRKSNSLRLVVSFPVSSPIRTSVFSRYGGAPRSSGPADSKRSDDEFGLDVSAIRSNNVR 120

Query: 121 LIDAAQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQQ 180
           LIDA QNMVG+VSKAQAIQMAEDAELDLVV+SPDADPPVVRIMDYNKYRYELQKKKRGQQ
Sbjct: 121 LIDAQQNMVGIVSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQKKKRGQQ 180

Query: 181 KKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAIE 240
           KKSAATRMDLKELKMGYNIDQHDYSVRL+AA+KFLKDGDKVKVIVNLKGRENEFRNIAIE
Sbjct: 181 KKSAATRMDLKELKMGYNIDQHDYSVRLRAAQKFLKDGDKVKVIVNLKGRENEFRNIAIE 240

Query: 241 LIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQE-PPKKKDKPAVNEVSAGV 294
           LIRRFQEE+GELATEEAKNFRDRNIFIVLVPNKAVLQKAQE PPKKKDKPAVNEVSAGV
Sbjct: 241 LIRRFQEEIGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEQPPKKKDKPAVNEVSAGV 295

BLAST of Chy5G100290 vs. NCBI nr
Match: XP_022158178.1 (translation initiation factor IF3-4, chloroplastic [Momordica charantia] >XP_022158179.1 translation initiation factor IF3-4, chloroplastic [Momordica charantia])

HSP 1 Score: 455 bits (1170), Expect = 3.54e-159
Identity = 247/294 (84.01%), Postives = 269/294 (91.50%), Query Frame = 0

Query: 1   MAGLSGSFPFKSLLSQSSAAATTTTSSAPPAATSSSSTTPFRTSFPSLPISSSNSNAFKF 60
           MAGL+  FPFK LLSQSSA   TT+S+A  +++S+++  PFRTS   LP+SSS SNAF F
Sbjct: 1   MAGLTSRFPFKPLLSQSSA---TTSSAAASSSSSAAAAPPFRTSISPLPLSSSESNAFGF 60

Query: 61  RLHKSNSLRLLFSSPIRTSVFARLGGASRSSGPPDSKRSNDEIGLDISAIKSTNVRLIDA 120
           RLH SNSLRL+ SSPIR SVFAR GGA RSSGP DSKRS+D+ GLD+SAI+S NVRLIDA
Sbjct: 61  RLHNSNSLRLIVSSPIRRSVFARFGGAPRSSGPADSKRSDDDFGLDVSAIRSNNVRLIDA 120

Query: 121 AQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQQKKSA 180
            QNMVG+VSKAQAIQMAEDAELDLVV+SPDADPPVVRIMDYNKYRYELQKKKRGQQKKSA
Sbjct: 121 QQNMVGIVSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQKKKRGQQKKSA 180

Query: 181 ATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAIELIRR 240
           ATRMDLKELKMGYNIDQHDYSVRLKAA++FLKDGDKVKVIVNLKGRENEFRNIAIELIRR
Sbjct: 181 ATRMDLKELKMGYNIDQHDYSVRLKAARRFLKDGDKVKVIVNLKGRENEFRNIAIELIRR 240

Query: 241 FQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKPAVNEVSAGV 294
           FQ+E+GELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKD PA+NEVSAGV
Sbjct: 241 FQDEIGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDNPAINEVSAGV 291

BLAST of Chy5G100290 vs. NCBI nr
Match: KAG6599141.1 (Translation initiation factor IF3-2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 429 bits (1104), Expect = 6.50e-149
Identity = 237/295 (80.34%), Postives = 263/295 (89.15%), Query Frame = 0

Query: 1   MAGLSGSFPFKSLLSQSSAAATTTTSSAPPAATSSSSTTPFRTSFPSLPISSSNSNAFKF 60
           MAGLSG FPFKS+ SQSSA AT TT+++  +++SSSS TPFRTS   LP+ SS SNAF+F
Sbjct: 1   MAGLSGRFPFKSISSQSSATATATTTTSSSSSSSSSSATPFRTSIFPLPLPSSKSNAFRF 60

Query: 61  RLHKSNSLRLLFSSPIRTSVFARLGGASRSSGPPDSKRSNDEIGLDISAIKSTNVRLIDA 120
           RLH SNSLRL+ SSPIRTSVFAR GGA RSSGP  SK S D + LD+S+I+S NVRLID 
Sbjct: 61  RLHNSNSLRLV-SSPIRTSVFARYGGAPRSSGPAGSKHSEDSLALDVSSIRSNNVRLIDE 120

Query: 121 AQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQQKKSA 180
            QNMVG+V K+QAIQMAEDAELDLVV+SPDADPPVVRIMDY+KYRY+LQKKKR QQKKSA
Sbjct: 121 QQNMVGIVPKSQAIQMAEDAELDLVVLSPDADPPVVRIMDYSKYRYDLQKKKREQQKKSA 180

Query: 181 ATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAIELIRR 240
           ATRMDLKELKMGY IDQHDYSVRL+AAKKFL+DGDKVKVIVNLKGRENEFR IAIEL++R
Sbjct: 181 ATRMDLKELKMGYKIDQHDYSVRLRAAKKFLQDGDKVKVIVNLKGRENEFRKIAIELVKR 240

Query: 241 FQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKPA-VNEVSAGV 294
           FQEE+GELATEEAKNFRDRN+FIVL PNKAVLQK+QEPPKKKDKPA VNEVSA V
Sbjct: 241 FQEELGELATEEAKNFRDRNVFIVLAPNKAVLQKSQEPPKKKDKPAAVNEVSARV 294

BLAST of Chy5G100290 vs. TAIR 10
Match: AT4G30690.1 (Translation initiation factor 3 protein )

HSP 1 Score: 288.5 bits (737), Expect = 6.1e-78
Identity = 154/211 (72.99%), Postives = 181/211 (85.78%), Query Frame = 0

Query: 85  GGASRSSGP--PDSKRSNDEIGLDISAIKSTNVRLIDAAQNMVGVVSKAQAIQMAEDAEL 144
           GG SR  G      K S D+  LDISAI+S  VRLID  QNM+G+VSK +A++ AEDAEL
Sbjct: 64  GGGSRFPGDRRGRQKESEDDDSLDISAIRSATVRLIDDQQNMIGLVSKEEAVRRAEDAEL 123

Query: 145 DLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQQKKSAATRMDLKELKMGYNIDQHDYSV 204
           DLV++SPDADPPVVR+MDY+KYRYE QK+K+ QQKK+  TRMDLKELKMGYNIDQHDYSV
Sbjct: 124 DLVILSPDADPPVVRMMDYSKYRYEQQKRKKEQQKKT--TRMDLKELKMGYNIDQHDYSV 183

Query: 205 RLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAIELIRRFQEEVGELATEEAKNFRDRNIF 264
           R++AA+KFL+DGDKVKVIVN+KGRENEFRNIAIEL+RRFQ E+GEL TEE+KNFRDRN+F
Sbjct: 184 RMRAARKFLQDGDKVKVIVNMKGRENEFRNIAIELLRRFQTEIGELGTEESKNFRDRNLF 243

Query: 265 IVLVPNKAVLQKAQE-PPKKKDKPAVNEVSA 293
           IVLVPNK V++K QE PPKKK KPA ++VSA
Sbjct: 244 IVLVPNKEVIRKVQEPPPKKKKKPADDKVSA 272

BLAST of Chy5G100290 vs. TAIR 10
Match: AT2G24060.1 (Translation initiation factor 3 protein )

HSP 1 Score: 275.4 bits (703), Expect = 5.3e-74
Identity = 165/274 (60.22%), Postives = 203/274 (74.09%), Query Frame = 0

Query: 35  SSSTTPFRTSFPSLPISSSNSNAF-------KFRLHKSN-SLRLLFSSPIR----TSVFA 94
           +SST  F   F  +  + S+ + F         RL K+  S   L  SP R    T  F 
Sbjct: 5   TSSTVGFNAVFTGITKTVSSHSLFSVDSKLCSLRLSKTELSFTNLTPSPRRAFAVTCRFG 64

Query: 95  RLGGASRSSGPPDSKRSND---EIGLDISAIKSTNVRLIDAAQNMVGVVSKAQAIQMAED 154
             GG  R SG     R  +   +  LDIS+I+S  VRLID  QNM+G+VSK +A++MA+D
Sbjct: 65  GGGGGYRFSGDNRRGRPKEAEIDEALDISSIRSATVRLIDGQQNMLGLVSKDEAVRMADD 124

Query: 155 AELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQQKKSAATRMDLKELKMGYNIDQHD 214
           AELDLV++SPDADPPVV++MDY+KYRYE QK+K+ QQKK+  TRMDLKELKMGYNIDQHD
Sbjct: 125 AELDLVILSPDADPPVVKMMDYSKYRYEQQKRKKDQQKKT--TRMDLKELKMGYNIDQHD 184

Query: 215 YSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAIELIRRFQEEVGELATEEAKNFRDR 274
           YSVRL+AA+KFL+DGDKVKVIV++KGRENEFRNIAIEL+RRFQ E+GELATEE+KNFRDR
Sbjct: 185 YSVRLRAAQKFLQDGDKVKVIVSMKGRENEFRNIAIELLRRFQTEIGELATEESKNFRDR 244

Query: 275 NIFIVLVPNKAVLQKAQEPP-KKKDKPAVNEVSA 293
           N+FI+LVPNK +++K QEPP +KK K A NE SA
Sbjct: 245 NMFIILVPNKEMIRKPQEPPTRKKKKTAENEASA 276

BLAST of Chy5G100290 vs. TAIR 10
Match: AT4G30690.2 (Translation initiation factor 3 protein )

HSP 1 Score: 235.3 bits (599), Expect = 6.1e-62
Identity = 131/211 (62.09%), Postives = 155/211 (73.46%), Query Frame = 0

Query: 85  GGASRSSGP--PDSKRSNDEIGLDISAIKSTNVRLIDAAQNMVGVVSKAQAIQMAEDAEL 144
           GG SR  G      K S D+  LDISAI+S  VRLID  QNM+G+VSK +A++ AEDAEL
Sbjct: 64  GGGSRFPGDRRGRQKESEDDDSLDISAIRSATVRLIDDQQNMIGLVSKEEAVRRAEDAEL 123

Query: 145 DLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQQKKSAATRMDLKELKMGYNIDQHDYSV 204
           DLV++SPDADPPVVR+MDY+                              YNIDQHDYSV
Sbjct: 124 DLVILSPDADPPVVRMMDYS------------------------------YNIDQHDYSV 183

Query: 205 RLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAIELIRRFQEEVGELATEEAKNFRDRNIF 264
           R++AA+KFL+DGDKVKVIVN+KGRENEFRNIAIEL+RRFQ E+GEL TEE+KNFRDRN+F
Sbjct: 184 RMRAARKFLQDGDKVKVIVNMKGRENEFRNIAIELLRRFQTEIGELGTEESKNFRDRNLF 243

Query: 265 IVLVPNKAVLQKAQE-PPKKKDKPAVNEVSA 293
           IVLVPNK V++K QE PPKKK KPA ++VSA
Sbjct: 244 IVLVPNKEVIRKVQEPPPKKKKKPADDKVSA 244

BLAST of Chy5G100290 vs. TAIR 10
Match: AT1G34360.1 (translation initiation factor 3 (IF-3) family protein )

HSP 1 Score: 55.1 bits (131), Expect = 1.1e-07
Identity = 59/191 (30.89%), Postives = 95/191 (49.74%), Query Frame = 0

Query: 95  DSKRSNDEIGLDISAIKSTNVRLIDAAQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPP 154
           D  R N++I  D        VRL+    +   VVS  +A++ A++ + DLV V  DA PP
Sbjct: 82  DGPRLNEKITGDY-------VRLVSEEGHC--VVSLREALRRAKELQCDLVEVQRDAKPP 141

Query: 155 VVRIMDYNKYRYELQK--KKRGQQKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLK 214
           V +I+ Y+   Y+  K  K+R + K++ A R D+KE++    I+  D   +   A K ++
Sbjct: 142 VCKIVKYSLELYKKAKVGKERAKAKRAEAIRPDIKEIRFTPKIEAKDLKFKSDQALKLME 201

Query: 215 DGDKVKVI-VNLKGRENEFR-NIAIELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKA 274
            G +VK + V  K +  E      +EL+ RF   +G+ A  E+    DR   +V+V +  
Sbjct: 202 SGYRVKCLAVPDKDKHKELEPEKLLELLFRFTCFIGD-ALVESWPEADRKGAVVIVRHAK 257

Query: 275 VLQKAQEPPKK 282
                  PPKK
Sbjct: 262 F-----GPPKK 257

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94B528.5e-7772.99Translation initiation factor IF3-4, chloroplastic OS=Arabidopsis thaliana OX=37... [more]
O822347.5e-7360.22Translation initiation factor IF3-2, chloroplastic OS=Arabidopsis thaliana OX=37... [more]
B0JKQ11.2e-3040.91Translation initiation factor IF-3 OS=Microcystis aeruginosa (strain NIES-843) O... [more]
Q8R9C27.8e-3042.86Translation initiation factor IF-3 OS=Caldanaerobacter subterraneus subsp. tengc... [more]
Q0IDZ57.8e-3044.20Translation initiation factor IF-3 OS=Synechococcus sp. (strain CC9311) OX=64471... [more]
Match NameE-valueIdentityDescription
A0A0A0LH347.4e-14095.32Translation initiation factor IF-3 OS=Cucumis sativus OX=3659 GN=Csa_2G036070 PE... [more]
A0A1S3BV826.5e-13692.64Translation initiation factor IF-3 OS=Cucumis melo OX=3656 GN=LOC103493630 PE=3 ... [more]
A0A6J1DYM53.3e-12484.01Translation initiation factor IF-3 OS=Momordica charantia OX=3673 GN=LOC11102471... [more]
A0A6J1KDY97.4e-11681.02Translation initiation factor IF-3 OS=Cucurbita maxima OX=3661 GN=LOC111493999 P... [more]
A0A6J1G3Y59.7e-11680.68Translation initiation factor IF-3 OS=Cucurbita moschata OX=3662 GN=LOC111450575... [more]
Match NameE-valueIdentityDescription
XP_004144726.11.30e-17995.32translation initiation factor IF3-4, chloroplastic [Cucumis sativus] >KGN61058.1... [more]
XP_008452687.11.83e-17492.64PREDICTED: translation initiation factor IF-3 [Cucumis melo][more]
XP_038891171.18.65e-16186.96translation initiation factor IF3-4, chloroplastic [Benincasa hispida][more]
XP_022158178.13.54e-15984.01translation initiation factor IF3-4, chloroplastic [Momordica charantia] >XP_022... [more]
KAG6599141.16.50e-14980.34Translation initiation factor IF3-2, chloroplastic, partial [Cucurbita argyrospe... [more]
Match NameE-valueIdentityDescription
AT4G30690.16.1e-7872.99Translation initiation factor 3 protein [more]
AT2G24060.15.3e-7460.22Translation initiation factor 3 protein [more]
AT4G30690.26.1e-6262.09Translation initiation factor 3 protein [more]
AT1G34360.11.1e-0730.89translation initiation factor 3 (IF-3) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019815Translation initiation factor 3, C-terminalPFAMPF00707IF3_Ccoord: 185..268
e-value: 4.3E-23
score: 81.1
IPR001288Translation initiation factor 3TIGRFAMTIGR00168TIGR00168coord: 109..269
e-value: 2.8E-47
score: 158.4
IPR001288Translation initiation factor 3PANTHERPTHR10938TRANSLATION INITIATION FACTOR IF-3coord: 60..287
IPR001288Translation initiation factor 3HAMAPMF_00080IF_3coord: 87..270
score: 22.6852
IPR036788Translation initiation factor 3 (IF-3), C-terminal domain superfamilyGENE3D3.30.110.10coord: 174..270
e-value: 1.7E-23
score: 84.4
IPR036788Translation initiation factor 3 (IF-3), C-terminal domain superfamilySUPERFAMILY55200Translation initiation factor IF3, C-terminal domaincoord: 184..269
IPR036787Translation initiation factor 3 (IF-3), N-terminal domain superfamilyGENE3D3.10.20.80coord: 108..173
e-value: 3.7E-27
score: 96.2
IPR036787Translation initiation factor 3 (IF-3), N-terminal domain superfamilySUPERFAMILY54364Translation initiation factor IF3, N-terminal domaincoord: 109..177
IPR019814Translation initiation factor 3, N-terminalPFAMPF05198IF3_Ncoord: 109..173
e-value: 1.5E-25
score: 89.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 274..294
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 19..41
NoneNo IPR availablePANTHERPTHR10938:SF3TRANSLATION INITIATION FACTOR IF3-2, CHLOROPLASTICcoord: 60..287
IPR019813Translation initiation factor 3, conserved sitePROSITEPS00938IF3coord: 157..170

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy5G100290.1Chy5G100290.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032790 ribosome disassembly
biological_process GO:0006413 translational initiation
cellular_component GO:0009507 chloroplast
molecular_function GO:0043022 ribosome binding
molecular_function GO:0003743 translation initiation factor activity