Chy5G100200 (gene) Cucumber (hystrix) v1

Overview
NameChy5G100200
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionDUF4220 domain-containing protein
LocationchrH05: 14429361 .. 14431388 (-)
RNA-Seq ExpressionChy5G100200
SyntenyChy5G100200
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTTCTGAATTATTTGACCTCATTGTACCAGAACATATAAGTTTCATATGGGGTTACTGGGGCATTGAGTTGCTAGTATTGGCAAACTTTGTGTTCCAAGTAATCCTAACTTTCAATGGAAGTCGTAGAAGGCACACACCAGGAAATAAGCTCAGCTTAATTGTTTGGTTTTCCTACTTATTAGCTGCTAAAATTGCAACTGTTGTTCTGGGCAAGCTCACAACAATTGATATAGGGCGTGAACAGCGCAATACGCACACACAAGTCCAGGCTTTGTTGGCACCACTAATGTTCATGCAAATAGGAAATCCAGATACAATCACGGCCTACTCAATTGAAGACAACCAACTTGGAGTGAGGCAAGTTTTCAGTATGGTAATCCAAGTGGGAATTATGTTTTACATTCTAGTAAGGTCATGGACAGATTCCAAAACATCGTTTCTTTACTTGCCGATGTCTTTGGCTGGAATAATCAAGTATGGTGAGACTTCATGGGCTCTGAAATCAGCACTTAATGGCAACTTTGGCTTCACAATTGCTGATTTCTTCAAATATCACGAAGTAGCTGATTTGTTTAACAAATTACCACAGGGAGAGAATGAACTTCCAGAGGCAAACTTGATCTTGAGGGCCTACTACAGATTTTGCTGCCTAAAACCTCATCTTGAAAATTGGCTTTACTATCCTCCAACTGATTGTGATCAAGATAAACTATACATTAAAGAATGTGGGTATGAAGATGTGTTCAGAATTACAGATGTTGAATTGGGTTTCATGTATGATGCCCTCTACACTAAGGCACCTGTTGTATACACTCGTAAGGGTTTGATTCTTCGTTTAATAAGTTTGCTTAGCGTAATTGCCACACTAATTGGATTTTCTGTATTGTTCAAGGATGCATTTGTGTACAATATTAGTATTGGCTTCATCCATTTTGTGTTGATAGCAGCTTTGATAATTGAGATTTATCAGATCTTGAGACTACCATTTACAGATTGGGCTATAGTACAGATGATAAGGCACCATGAAGCTTTCCCAATCCTTCGGGGTTTCTTGCGGTCTCTATCCCCTCAGTCGGCAACTTGGAGAAGGTGGTCTAACACAATGGGGCAGTTCAATCTTTTAGATTTCTGCTTGCAAACCAAGCATCGGAACTACAGCAGAATTAAAATTCTCCGCTATATGGGGATGGATATGAAACTCCGAAAGCAATTGAGTTTGGATCGAATTGACGTCCATCCAGAGGTGAAAGAATTTGTGGTGACAGAACTGAAAGAGATAGAGGCGATCAAAGGACAAGAAGAATTTGATCAGAGAGGCCAATGGACAATCGATAGGTACAAAACTTTACTGAACCTCAACAACGAAAACAAATTGATTAAAGCAATTGAAACAACTGTATCAAAGAGACCCTTTGATAAGTGCATCTTCATATGGCACATCACAACAAATATTTTCTATAACATCGAAGTTCATCGTGATACTAGTGTAGGAAATAAAACGGAAGCTATCATGAGTCTATCAGATTATATGATGTATCTTGTGGTGACTCGTTCCCACGTGCTATCAACAACAACTGCAGATATAATATTTGATCATTCATGTGTGAAACTCGGGAAGTTCACAAGAACTGGACGTCTAAAGAAAGAGGACATCTGCAATGATATTCTGAAATTAAAGAAAGAGAGCATCCTTCATGCAAGAGAGCCACATGAACCAATTGAATCAGAAGCAGAAAAAGTAGTTGTAGGAAATTGGCATCTGATGAAGGATGTAAAAGAACTTGCAGACTGCTTATTGACACTGAGCAACGAGAACAAGTGGAAGCTAATTGGCAGCATGTGGTTTGAGATGTTGGGATATGCTGCAAGTAAATGTGAAATGGAATATCATTCAGAACACATCAGACAAGGTGGTGAATTGATCACTCATGTTTGGCTTTTGATAGCTCATAATGTTACCAAATACAGTTCATATGAATACCATGCGGGAGGTCAAGATGAAGAGACTCCAGCTACTTCTTAG

mRNA sequence

ATGTTTTCTGAATTATTTGACCTCATTGTACCAGAACATATAAGTTTCATATGGGGTTACTGGGGCATTGAGTTGCTAGTATTGGCAAACTTTGTGTTCCAAGTAATCCTAACTTTCAATGGAAGTCGTAGAAGGCACACACCAGGAAATAAGCTCAGCTTAATTGTTTGGTTTTCCTACTTATTAGCTGCTAAAATTGCAACTGTTGTTCTGGGCAAGCTCACAACAATTGATATAGGGCGTGAACAGCGCAATACGCACACACAAGTCCAGGCTTTGTTGGCACCACTAATGTTCATGCAAATAGGAAATCCAGATACAATCACGGCCTACTCAATTGAAGACAACCAACTTGGAGTGAGGCAAGTTTTCAGTATGGTAATCCAAGTGGGAATTATGTTTTACATTCTAGTAAGGTCATGGACAGATTCCAAAACATCGTTTCTTTACTTGCCGATGTCTTTGGCTGGAATAATCAAGTATGGTGAGACTTCATGGGCTCTGAAATCAGCACTTAATGGCAACTTTGGCTTCACAATTGCTGATTTCTTCAAATATCACGAAGTAGCTGATTTGTTTAACAAATTACCACAGGGAGAGAATGAACTTCCAGAGGCAAACTTGATCTTGAGGGCCTACTACAGATTTTGCTGCCTAAAACCTCATCTTGAAAATTGGCTTTACTATCCTCCAACTGATTGTGATCAAGATAAACTATACATTAAAGAATGTGGGTATGAAGATGTGTTCAGAATTACAGATGTTGAATTGGGTTTCATGTATGATGCCCTCTACACTAAGGCACCTGTTGTATACACTCGTAAGGGTTTGATTCTTCGTTTAATAAGTTTGCTTAGCGTAATTGCCACACTAATTGGATTTTCTGTATTGTTCAAGGATGCATTTGTGTACAATATTAGTATTGGCTTCATCCATTTTGTGTTGATAGCAGCTTTGATAATTGAGATTTATCAGATCTTGAGACTACCATTTACAGATTGGGCTATAGTACAGATGATAAGGCACCATGAAGCTTTCCCAATCCTTCGGGGTTTCTTGCGGTCTCTATCCCCTCAGTCGGCAACTTGGAGAAGGTGGTCTAACACAATGGGGCAGTTCAATCTTTTAGATTTCTGCTTGCAAACCAAGCATCGGAACTACAGCAGAATTAAAATTCTCCGCTATATGGGGATGGATATGAAACTCCGAAAGCAATTGAGTTTGGATCGAATTGACGTCCATCCAGAGGTGAAAGAATTTGTGGTGACAGAACTGAAAGAGATAGAGGCGATCAAAGGACAAGAAGAATTTGATCAGAGAGGCCAATGGACAATCGATAGGTACAAAACTTTACTGAACCTCAACAACGAAAACAAATTGATTAAAGCAATTGAAACAACTGTATCAAAGAGACCCTTTGATAAGTGCATCTTCATATGGCACATCACAACAAATATTTTCTATAACATCGAAGTTCATCGTGATACTAGTGTAGGAAATAAAACGGAAGCTATCATGAGTCTATCAGATTATATGATGTATCTTGTGGTGACTCGTTCCCACGTGCTATCAACAACAACTGCAGATATAATATTTGATCATTCATGTGTGAAACTCGGGAAGTTCACAAGAACTGGACGTCTAAAGAAAGAGGACATCTGCAATGATATTCTGAAATTAAAGAAAGAGAGCATCCTTCATGCAAGAGAGCCACATGAACCAATTGAATCAGAAGCAGAAAAAGTAGTTGTAGGAAATTGGCATCTGATGAAGGATGTAAAAGAACTTGCAGACTGCTTATTGACACTGAGCAACGAGAACAAGTGGAAGCTAATTGGCAGCATGTGGTTTGAGATGTTGGGATATGCTGCAAGTAAATGTGAAATGGAATATCATTCAGAACACATCAGACAAGGTGGTGAATTGATCACTCATGTTTGGCTTTTGATAGCTCATAATGTTACCAAATACAGTTCATATGAATACCATGCGGGAGGTCAAGATGAAGAGACTCCAGCTACTTCTTAG

Coding sequence (CDS)

ATGTTTTCTGAATTATTTGACCTCATTGTACCAGAACATATAAGTTTCATATGGGGTTACTGGGGCATTGAGTTGCTAGTATTGGCAAACTTTGTGTTCCAAGTAATCCTAACTTTCAATGGAAGTCGTAGAAGGCACACACCAGGAAATAAGCTCAGCTTAATTGTTTGGTTTTCCTACTTATTAGCTGCTAAAATTGCAACTGTTGTTCTGGGCAAGCTCACAACAATTGATATAGGGCGTGAACAGCGCAATACGCACACACAAGTCCAGGCTTTGTTGGCACCACTAATGTTCATGCAAATAGGAAATCCAGATACAATCACGGCCTACTCAATTGAAGACAACCAACTTGGAGTGAGGCAAGTTTTCAGTATGGTAATCCAAGTGGGAATTATGTTTTACATTCTAGTAAGGTCATGGACAGATTCCAAAACATCGTTTCTTTACTTGCCGATGTCTTTGGCTGGAATAATCAAGTATGGTGAGACTTCATGGGCTCTGAAATCAGCACTTAATGGCAACTTTGGCTTCACAATTGCTGATTTCTTCAAATATCACGAAGTAGCTGATTTGTTTAACAAATTACCACAGGGAGAGAATGAACTTCCAGAGGCAAACTTGATCTTGAGGGCCTACTACAGATTTTGCTGCCTAAAACCTCATCTTGAAAATTGGCTTTACTATCCTCCAACTGATTGTGATCAAGATAAACTATACATTAAAGAATGTGGGTATGAAGATGTGTTCAGAATTACAGATGTTGAATTGGGTTTCATGTATGATGCCCTCTACACTAAGGCACCTGTTGTATACACTCGTAAGGGTTTGATTCTTCGTTTAATAAGTTTGCTTAGCGTAATTGCCACACTAATTGGATTTTCTGTATTGTTCAAGGATGCATTTGTGTACAATATTAGTATTGGCTTCATCCATTTTGTGTTGATAGCAGCTTTGATAATTGAGATTTATCAGATCTTGAGACTACCATTTACAGATTGGGCTATAGTACAGATGATAAGGCACCATGAAGCTTTCCCAATCCTTCGGGGTTTCTTGCGGTCTCTATCCCCTCAGTCGGCAACTTGGAGAAGGTGGTCTAACACAATGGGGCAGTTCAATCTTTTAGATTTCTGCTTGCAAACCAAGCATCGGAACTACAGCAGAATTAAAATTCTCCGCTATATGGGGATGGATATGAAACTCCGAAAGCAATTGAGTTTGGATCGAATTGACGTCCATCCAGAGGTGAAAGAATTTGTGGTGACAGAACTGAAAGAGATAGAGGCGATCAAAGGACAAGAAGAATTTGATCAGAGAGGCCAATGGACAATCGATAGGTACAAAACTTTACTGAACCTCAACAACGAAAACAAATTGATTAAAGCAATTGAAACAACTGTATCAAAGAGACCCTTTGATAAGTGCATCTTCATATGGCACATCACAACAAATATTTTCTATAACATCGAAGTTCATCGTGATACTAGTGTAGGAAATAAAACGGAAGCTATCATGAGTCTATCAGATTATATGATGTATCTTGTGGTGACTCGTTCCCACGTGCTATCAACAACAACTGCAGATATAATATTTGATCATTCATGTGTGAAACTCGGGAAGTTCACAAGAACTGGACGTCTAAAGAAAGAGGACATCTGCAATGATATTCTGAAATTAAAGAAAGAGAGCATCCTTCATGCAAGAGAGCCACATGAACCAATTGAATCAGAAGCAGAAAAAGTAGTTGTAGGAAATTGGCATCTGATGAAGGATGTAAAAGAACTTGCAGACTGCTTATTGACACTGAGCAACGAGAACAAGTGGAAGCTAATTGGCAGCATGTGGTTTGAGATGTTGGGATATGCTGCAAGTAAATGTGAAATGGAATATCATTCAGAACACATCAGACAAGGTGGTGAATTGATCACTCATGTTTGGCTTTTGATAGCTCATAATGTTACCAAATACAGTTCATATGAATACCATGCGGGAGGTCAAGATGAAGAGACTCCAGCTACTTCTTAG

Protein sequence

MFSELFDLIVPEHISFIWGYWGIELLVLANFVFQVILTFNGSRRRHTPGNKLSLIVWFSYLLAAKIATVVLGKLTTIDIGREQRNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGVRQVFSMVIQVGIMFYILVRSWTDSKTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFTIADFFKYHEVADLFNKLPQGENELPEANLILRAYYRFCCLKPHLENWLYYPPTDCDQDKLYIKECGYEDVFRITDVELGFMYDALYTKAPVVYTRKGLILRLISLLSVIATLIGFSVLFKDAFVYNISIGFIHFVLIAALIIEIYQILRLPFTDWAIVQMIRHHEAFPILRGFLRSLSPQSATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYMGMDMKLRKQLSLDRIDVHPEVKEFVVTELKEIEAIKGQEEFDQRGQWTIDRYKTLLNLNNENKLIKAIETTVSKRPFDKCIFIWHITTNIFYNIEVHRDTSVGNKTEAIMSLSDYMMYLVVTRSHVLSTTTADIIFDHSCVKLGKFTRTGRLKKEDICNDILKLKKESILHAREPHEPIESEAEKVVVGNWHLMKDVKELADCLLTLSNENKWKLIGSMWFEMLGYAASKCEMEYHSEHIRQGGELITHVWLLIAHNVTKYSSYEYHAGGQDEETPATS*
Homology
BLAST of Chy5G100200 vs. ExPASy TrEMBL
Match: A0A0A0LK45 (DUF4220 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G036650 PE=4 SV=1)

HSP 1 Score: 1337.0 bits (3459), Expect = 0.0e+00
Identity = 661/675 (97.93%), Postives = 670/675 (99.26%), Query Frame = 0

Query: 1   MFSELFDLIVPEHISFIWGYWGIELLVLANFVFQVILTFNGSRRRHTPGNKLSLIVWFSY 60
           MFSELFDLIVPEHISFIWGYWGIELLVLANFVFQVILTFNGSRRRHTPGN+LSLIVWFSY
Sbjct: 1   MFSELFDLIVPEHISFIWGYWGIELLVLANFVFQVILTFNGSRRRHTPGNRLSLIVWFSY 60

Query: 61  LLAAKIATVVLGKLTTIDIGREQRNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120
           LLAAKIATVVLGKLTTIDIG EQRNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGV
Sbjct: 61  LLAAKIATVVLGKLTTIDIGHEQRNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120

Query: 121 RQVFSMVIQVGIMFYILVRSWTDSKTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFTI 180
           RQVFSMVIQVGIMFYILVRSWTDSKTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFTI
Sbjct: 121 RQVFSMVIQVGIMFYILVRSWTDSKTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFTI 180

Query: 181 ADFFKYHEVADLFNKLPQGENELPEANLILRAYYRFCCLKPHLENWLYYPPTDCDQDKLY 240
           ADFFKYHEVADLFNKLPQGENELPEANLILRAYYRFCCLKPHLENWLYYPPTDCDQ KL+
Sbjct: 181 ADFFKYHEVADLFNKLPQGENELPEANLILRAYYRFCCLKPHLENWLYYPPTDCDQGKLH 240

Query: 241 IKECGYEDVFRITDVELGFMYDALYTKAPVVYTRKGLILRLISLLSVIATLIGFSVLFKD 300
           IKECGYEDVFRITDVELGFMYDALYTKAPVVYTRKGLILRLISLLSVIATL+GFSVLFKD
Sbjct: 241 IKECGYEDVFRITDVELGFMYDALYTKAPVVYTRKGLILRLISLLSVIATLVGFSVLFKD 300

Query: 301 AFVYNISIGFIHFVLIAALIIEIYQILRLPFTDWAIVQMIRHHEAFPILRGFLRSLSPQS 360
           AFVYNISIGFIHFVLIAALIIEIYQILRLPFTDWAIVQM+RHHEAFPILRGFLRSLSPQS
Sbjct: 301 AFVYNISIGFIHFVLIAALIIEIYQILRLPFTDWAIVQMVRHHEAFPILRGFLRSLSPQS 360

Query: 361 ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYMGMDMKLRKQLSLDRIDVHPEVKEF 420
           ATWRRWSNTMGQFNLL+FCLQTKHRNYSRIKILRYMGMDMKLRKQLSLDRIDV PEVKEF
Sbjct: 361 ATWRRWSNTMGQFNLLEFCLQTKHRNYSRIKILRYMGMDMKLRKQLSLDRIDVSPEVKEF 420

Query: 421 VVTELKEIEAIKGQEEFDQRGQWTIDRYKTLLNLNNENKLIKAIETTVSKRPFDKCIFIW 480
           VVTEL+EIEAIKG+EEFDQRGQWTI+RYKTLLNLNNENKLIKAIETTVSKRPFDKCIFIW
Sbjct: 421 VVTELREIEAIKGEEEFDQRGQWTINRYKTLLNLNNENKLIKAIETTVSKRPFDKCIFIW 480

Query: 481 HITTNIFYNIEVHRDTSVGNKTEAIMSLSDYMMYLVVTRSHVLSTTTADIIFDHSCVKLG 540
           HITTNIFYNIE +RDTSVGNKTEAIMSLSDYMMYLVVTRSHVLSTTTADIIFDHSCVKLG
Sbjct: 481 HITTNIFYNIEGYRDTSVGNKTEAIMSLSDYMMYLVVTRSHVLSTTTADIIFDHSCVKLG 540

Query: 541 KFTRTGRLKKEDICNDILKLKKESILHAREPHEPIESEAEKVVVGNWHLMKDVKELADCL 600
           KFTRTGRLKKEDICNDILKLKKESILHAREPHEPIESEAEKVVVGNWHLMKDVKELADCL
Sbjct: 541 KFTRTGRLKKEDICNDILKLKKESILHAREPHEPIESEAEKVVVGNWHLMKDVKELADCL 600

Query: 601 LTLSNENKWKLIGSMWFEMLGYAASKCEMEYHSEHIRQGGELITHVWLLIAHNVTKYSSY 660
           L LSNENKWKLIGSMWFEMLGYAASKCEMEYHSEHIRQGGELITHVWLLIAHNVTKYSSY
Sbjct: 601 LALSNENKWKLIGSMWFEMLGYAASKCEMEYHSEHIRQGGELITHVWLLIAHNVTKYSSY 660

Query: 661 EYHAGGQDEETPATS 676
           EYHAGGQDEETPATS
Sbjct: 661 EYHAGGQDEETPATS 675

BLAST of Chy5G100200 vs. ExPASy TrEMBL
Match: A0A1S4DZ97 (uncharacterized protein LOC103493508 OS=Cucumis melo OX=3656 GN=LOC103493508 PE=4 SV=1)

HSP 1 Score: 1310.0 bits (3389), Expect = 0.0e+00
Identity = 649/675 (96.15%), Postives = 659/675 (97.63%), Query Frame = 0

Query: 1   MFSELFDLIVPEHISFIWGYWGIELLVLANFVFQVILTFNGSRRRHTPGNKLSLIVWFSY 60
           MFSELFDLIVPEHISFIWGYWGIELLVLANFVFQVILTFNGSRRRHTPGN+LSLIVWFSY
Sbjct: 1   MFSELFDLIVPEHISFIWGYWGIELLVLANFVFQVILTFNGSRRRHTPGNRLSLIVWFSY 60

Query: 61  LLAAKIATVVLGKLTTIDIGREQRNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120
           LLAAKIATVVLGKLTTIDIGREQRNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGV
Sbjct: 61  LLAAKIATVVLGKLTTIDIGREQRNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120

Query: 121 RQVFSMVIQVGIMFYILVRSWTDSKTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFTI 180
           RQVFSMVIQVGIMFYILVRSWTDSKTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFTI
Sbjct: 121 RQVFSMVIQVGIMFYILVRSWTDSKTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFTI 180

Query: 181 ADFFKYHEVADLFNKLPQGENELPEANLILRAYYRFCCLKPHLENWLYYPPTDCDQDKLY 240
           ADFFKYHEVA+LFNKLPQGENELPEANLILRAYYRFCCLKPHLENWLYYPPTDCDQDKLY
Sbjct: 181 ADFFKYHEVANLFNKLPQGENELPEANLILRAYYRFCCLKPHLENWLYYPPTDCDQDKLY 240

Query: 241 IKECGYEDVFRITDVELGFMYDALYTKAPVVYTRKGLILRLISLLSVIATLIGFSVLFKD 300
           IK+CGYEDVFRITDVELGFMYDALYTKAPVVYTRKGLILR ISLLSVIATL GFSVLFKD
Sbjct: 241 IKDCGYEDVFRITDVELGFMYDALYTKAPVVYTRKGLILRFISLLSVIATLCGFSVLFKD 300

Query: 301 AFVYNISIGFIHFVLIAALIIEIYQILRLPFTDWAIVQMIRHHEAFPILRGFLRSLSPQS 360
           AFVYNISIGFIHFVLIAALIIEIYQILRLPFTDWAIVQM+RHHEAFPILRGFLRSL+PQS
Sbjct: 301 AFVYNISIGFIHFVLIAALIIEIYQILRLPFTDWAIVQMVRHHEAFPILRGFLRSLAPQS 360

Query: 361 ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYMGMDMKLRKQLSLDRIDVHPEVKEF 420
           ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRY GMDMKLRKQLSLDRIDVHPEV+EF
Sbjct: 361 ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYWGMDMKLRKQLSLDRIDVHPEVREF 420

Query: 421 VVTELKEIEAIKGQEEFDQRGQWTIDRYKTLLNLNNENKLIKAIETTVSKRPFDKCIFIW 480
           +VTEL+EIE IKGQEEFD RGQWTIDRYKT      ENKLIKAIETTV KRPFDKCIFIW
Sbjct: 421 LVTELREIEEIKGQEEFDNRGQWTIDRYKTKF----ENKLIKAIETTVLKRPFDKCIFIW 480

Query: 481 HITTNIFYNIEVHRDTSVGNKTEAIMSLSDYMMYLVVTRSHVLSTTTADIIFDHSCVKLG 540
           HITTNIFYNIE +RD SVGNKTEAIM LSDYMMYLVVTRSHVLSTTTADIIFDHSCVKLG
Sbjct: 481 HITTNIFYNIEGYRDNSVGNKTEAIMCLSDYMMYLVVTRSHVLSTTTADIIFDHSCVKLG 540

Query: 541 KFTRTGRLKKEDICNDILKLKKESILHAREPHEPIESEAEKVVVGNWHLMKDVKELADCL 600
           KFTRTGRLKKEDICNDIL LKKESILHAREPHEPIESEAEKVVVGNWHLMKDVKELADCL
Sbjct: 541 KFTRTGRLKKEDICNDILNLKKESILHAREPHEPIESEAEKVVVGNWHLMKDVKELADCL 600

Query: 601 LTLSNENKWKLIGSMWFEMLGYAASKCEMEYHSEHIRQGGELITHVWLLIAHNVTKYSSY 660
           LTLSNE+KWKLIGSMWFEMLGYAASKCEMEYHSEHIRQGGELITHVWLLIAHNVTKYSSY
Sbjct: 601 LTLSNEDKWKLIGSMWFEMLGYAASKCEMEYHSEHIRQGGELITHVWLLIAHNVTKYSSY 660

Query: 661 EYHAGGQDEETPATS 676
           EYHAGGQDEETPATS
Sbjct: 661 EYHAGGQDEETPATS 671

BLAST of Chy5G100200 vs. ExPASy TrEMBL
Match: A0A5A7TID1 (DUF4220 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G00210 PE=4 SV=1)

HSP 1 Score: 1310.0 bits (3389), Expect = 0.0e+00
Identity = 649/675 (96.15%), Postives = 659/675 (97.63%), Query Frame = 0

Query: 1   MFSELFDLIVPEHISFIWGYWGIELLVLANFVFQVILTFNGSRRRHTPGNKLSLIVWFSY 60
           MFSELFDLIVPEHISFIWGYWGIELLVLANFVFQVILTFNGSRRRHTPGN+LSLIVWFSY
Sbjct: 1   MFSELFDLIVPEHISFIWGYWGIELLVLANFVFQVILTFNGSRRRHTPGNRLSLIVWFSY 60

Query: 61  LLAAKIATVVLGKLTTIDIGREQRNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120
           LLAAKIATVVLGKLTTIDIGREQRNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGV
Sbjct: 61  LLAAKIATVVLGKLTTIDIGREQRNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120

Query: 121 RQVFSMVIQVGIMFYILVRSWTDSKTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFTI 180
           RQVFSMVIQVGIMFYILVRSWTDSKTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFTI
Sbjct: 121 RQVFSMVIQVGIMFYILVRSWTDSKTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFTI 180

Query: 181 ADFFKYHEVADLFNKLPQGENELPEANLILRAYYRFCCLKPHLENWLYYPPTDCDQDKLY 240
           ADFFKYHEVA+LFNKLPQGENELPEANLILRAYYRFCCLKPHLENWLYYPPTDCDQDKLY
Sbjct: 181 ADFFKYHEVANLFNKLPQGENELPEANLILRAYYRFCCLKPHLENWLYYPPTDCDQDKLY 240

Query: 241 IKECGYEDVFRITDVELGFMYDALYTKAPVVYTRKGLILRLISLLSVIATLIGFSVLFKD 300
           IK+CGYEDVFRITDVELGFMYDALYTKAPVVYTRKGLILR ISLLSVIATL GFSVLFKD
Sbjct: 241 IKDCGYEDVFRITDVELGFMYDALYTKAPVVYTRKGLILRFISLLSVIATLCGFSVLFKD 300

Query: 301 AFVYNISIGFIHFVLIAALIIEIYQILRLPFTDWAIVQMIRHHEAFPILRGFLRSLSPQS 360
           AFVYNISIGFIHFVLIAALIIEIYQILRLPFTDWAIVQM+RHHEAFPILRGFLRSL+PQS
Sbjct: 301 AFVYNISIGFIHFVLIAALIIEIYQILRLPFTDWAIVQMVRHHEAFPILRGFLRSLAPQS 360

Query: 361 ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYMGMDMKLRKQLSLDRIDVHPEVKEF 420
           ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRY GMDMKLRKQLSLDRIDVHPEV+EF
Sbjct: 361 ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYWGMDMKLRKQLSLDRIDVHPEVREF 420

Query: 421 VVTELKEIEAIKGQEEFDQRGQWTIDRYKTLLNLNNENKLIKAIETTVSKRPFDKCIFIW 480
           +VTEL+EIE IKGQEEFD RGQWTIDRYKT      ENKLIKAIETTV KRPFDKCIFIW
Sbjct: 421 LVTELREIEEIKGQEEFDNRGQWTIDRYKTKF----ENKLIKAIETTVLKRPFDKCIFIW 480

Query: 481 HITTNIFYNIEVHRDTSVGNKTEAIMSLSDYMMYLVVTRSHVLSTTTADIIFDHSCVKLG 540
           HITTNIFYNIE +RD SVGNKTEAIM LSDYMMYLVVTRSHVLSTTTADIIFDHSCVKLG
Sbjct: 481 HITTNIFYNIEGYRDNSVGNKTEAIMCLSDYMMYLVVTRSHVLSTTTADIIFDHSCVKLG 540

Query: 541 KFTRTGRLKKEDICNDILKLKKESILHAREPHEPIESEAEKVVVGNWHLMKDVKELADCL 600
           KFTRTGRLKKEDICNDIL LKKESILHAREPHEPIESEAEKVVVGNWHLMKDVKELADCL
Sbjct: 541 KFTRTGRLKKEDICNDILNLKKESILHAREPHEPIESEAEKVVVGNWHLMKDVKELADCL 600

Query: 601 LTLSNENKWKLIGSMWFEMLGYAASKCEMEYHSEHIRQGGELITHVWLLIAHNVTKYSSY 660
           LTLSNE+KWKLIGSMWFEMLGYAASKCEMEYHSEHIRQGGELITHVWLLIAHNVTKYSSY
Sbjct: 601 LTLSNEDKWKLIGSMWFEMLGYAASKCEMEYHSEHIRQGGELITHVWLLIAHNVTKYSSY 660

Query: 661 EYHAGGQDEETPATS 676
           EYHAGGQDEETPATS
Sbjct: 661 EYHAGGQDEETPATS 671

BLAST of Chy5G100200 vs. ExPASy TrEMBL
Match: A0A6J1KHN9 (uncharacterized protein LOC111493941 OS=Cucurbita maxima OX=3661 GN=LOC111493941 PE=4 SV=1)

HSP 1 Score: 1171.8 bits (3030), Expect = 0.0e+00
Identity = 580/675 (85.93%), Postives = 619/675 (91.70%), Query Frame = 0

Query: 1   MFSELFDLIVPEHISFIWGYWGIELLVLANFVFQVILTFNGSRRRHTPGNKLSLIVWFSY 60
           MFS+LF  I+P HISFIW  WGIELLV ANF+FQVILT+NGSRRRHTPG KLSL VWFSY
Sbjct: 1   MFSDLFLRILPGHISFIWNNWGIELLVFANFMFQVILTYNGSRRRHTPGYKLSLTVWFSY 60

Query: 61  LLAAKIATVVLGKLTTIDIGREQRNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120
           LLAAKIATVVLGKLTTIDIG E+RNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGV
Sbjct: 61  LLAAKIATVVLGKLTTIDIGHERRNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120

Query: 121 RQVFSMVIQVGIMFYILVRSWTDSKTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFTI 180
           RQVFSMVIQV IMFYILVRSWTDSKTSFLYLPMSLAGIIKYGETSWALKSAL+GN GFTI
Sbjct: 121 RQVFSMVIQVTIMFYILVRSWTDSKTSFLYLPMSLAGIIKYGETSWALKSALSGNLGFTI 180

Query: 181 ADFFKYHEVADLFNKLPQGENELPEANLILRAYYRFCCLKPHLENWLYYPPTDCDQDKLY 240
           ADFFKYHEVA+LF+KLPQ  NELPEA LILRAYYRFCCLKPHLENWLYYPPTDCD  KLY
Sbjct: 181 ADFFKYHEVANLFDKLPQ--NELPEAKLILRAYYRFCCLKPHLENWLYYPPTDCDHKKLY 240

Query: 241 IKECGYEDVFRITDVELGFMYDALYTKAPVVYTRKGLILRLISLLSVIATLIGFSVLFKD 300
           I +C YEDVFRITD ELGFMYDALYTKAPV+YTRKGLILR ISLLS+IATL GFSVLFKD
Sbjct: 241 IDDCEYEDVFRITDSELGFMYDALYTKAPVIYTRKGLILRFISLLSLIATLCGFSVLFKD 300

Query: 301 AFVYNISIGFIHFVLIAALIIEIYQILRLPFTDWAIVQMIRHHEAFPILRGFLRSLSPQS 360
           AFVYNIS+GFIH+VLIA+LIIEIYQI+R+PFTDWAIVQMIRHHE FPILRG L SL+PQS
Sbjct: 301 AFVYNISVGFIHYVLIASLIIEIYQIMRIPFTDWAIVQMIRHHETFPILRGLLHSLAPQS 360

Query: 361 ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYMGMDMKLRKQLSLDRIDVHPEVKEF 420
           ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIK+LR  G+DMKLRKQ+SLDRIDVHPEVKE 
Sbjct: 361 ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKLLRTWGLDMKLRKQMSLDRIDVHPEVKEL 420

Query: 421 VVTELKEIEAIKGQEEFDQRGQWTIDRYKTLLNLNNENKLIKAIETTVSKRPFDKCIFIW 480
           VVTEL+EIE IKGQEEF+QRGQWTIDRYKT L  N+++ LIKA+ETTV+KRPFDK IFIW
Sbjct: 421 VVTELREIEKIKGQEEFEQRGQWTIDRYKTKLKQNDDSILIKALETTVNKRPFDKSIFIW 480

Query: 481 HITTNIFYNIEVHRDTSVGNKTEAIMSLSDYMMYLVVTRSHVLSTTTADIIFDHSCVKLG 540
           HITTNIFY+I    DTS+GNK EAIMS+S+YMMYL+VTRSHVLSTTT +IIFDHSCVKLG
Sbjct: 481 HITTNIFYHIRRFHDTSIGNKMEAIMSISNYMMYLLVTRSHVLSTTTGEIIFDHSCVKLG 540

Query: 541 KFTRTGRLKKEDICNDILKLKKESILHAREPHEPIESEAEKVVVGNWHLMKDVKELADCL 600
           KFTRTG L KED CN +L L+KE  L A EPHEP  SEAEKVVVGNWHL+KDVK+LAD L
Sbjct: 541 KFTRTGHLNKEDACNALLNLQKEGTLQAEEPHEP--SEAEKVVVGNWHLLKDVKDLADSL 600

Query: 601 LTLSNENKWKLIGSMWFEMLGYAASKCEMEYHSEHIRQGGELITHVWLLIAHNVTKYSSY 660
           L LSNEN+W+LIGSMWFEMLGYAASKCEMEYHSEHIRQGGELITHVWLLIAHNVTKYSSY
Sbjct: 601 LFLSNENRWQLIGSMWFEMLGYAASKCEMEYHSEHIRQGGELITHVWLLIAHNVTKYSSY 660

Query: 661 EYHAGGQDEETPATS 676
           EYH GGQDEE  A S
Sbjct: 661 EYHVGGQDEEIAANS 671

BLAST of Chy5G100200 vs. ExPASy TrEMBL
Match: A0A6J1G3A8 (uncharacterized protein LOC111450396 OS=Cucurbita moschata OX=3662 GN=LOC111450396 PE=4 SV=1)

HSP 1 Score: 1147.9 bits (2968), Expect = 0.0e+00
Identity = 568/675 (84.15%), Postives = 611/675 (90.52%), Query Frame = 0

Query: 1   MFSELFDLIVPEHISFIWGYWGIELLVLANFVFQVILTFNGSRRRHTPGNKLSLIVWFSY 60
           MFS+LF  I+P HISFIW  WGIELLV ANFVFQVILT+NGSRRRHTPG KLSL VWFSY
Sbjct: 1   MFSDLFLRILPGHISFIWNNWGIELLVFANFVFQVILTYNGSRRRHTPGYKLSLTVWFSY 60

Query: 61  LLAAKIATVVLGKLTTIDIGREQRNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120
           LLAAKIATVVLGKLTTIDIG E+RNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGV
Sbjct: 61  LLAAKIATVVLGKLTTIDIGHERRNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120

Query: 121 RQVFSMVIQVGIMFYILVRSWTDSKTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFTI 180
           RQVFSM +QV IMFYILVRSWTDSKTSFLYLPMSLAGIIKYGETSWALKSAL+GN GFTI
Sbjct: 121 RQVFSMGVQVTIMFYILVRSWTDSKTSFLYLPMSLAGIIKYGETSWALKSALSGNLGFTI 180

Query: 181 ADFFKYHEVADLFNKLPQGENELPEANLILRAYYRFCCLKPHLENWLYYPPTDCDQDKLY 240
           ADFFKY EVA LF KLPQ  NELPEA LILRAYYRFCCLKPHLENWLYYPPTDCD  KLY
Sbjct: 181 ADFFKYQEVAKLFEKLPQ--NELPEAKLILRAYYRFCCLKPHLENWLYYPPTDCDHKKLY 240

Query: 241 IKECGYEDVFRITDVELGFMYDALYTKAPVVYTRKGLILRLISLLSVIATLIGFSVLFKD 300
           I +C YEDVFRITD ELGFMYDALYTKAPV+YTRKGLILR ISLLS+IATL GFSVLFKD
Sbjct: 241 IDDCEYEDVFRITDSELGFMYDALYTKAPVIYTRKGLILRFISLLSLIATLCGFSVLFKD 300

Query: 301 AFVYNISIGFIHFVLIAALIIEIYQILRLPFTDWAIVQMIRHHEAFPILRGFLRSLSPQS 360
           AFVYNIS+GFIH+VLIA+LIIE+YQI+R+PFTDWAIVQMIRHH+ FPILRG L SL+PQS
Sbjct: 301 AFVYNISVGFIHYVLIASLIIELYQIMRIPFTDWAIVQMIRHHKTFPILRGLLHSLAPQS 360

Query: 361 ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYMGMDMKLRKQLSLDRIDVHPEVKEF 420
           ATWRRWSNTMGQFNLLDFC+QTKHRNYSRIK+LR  GMDMKLRKQ+SLDRI+V PEVKE 
Sbjct: 361 ATWRRWSNTMGQFNLLDFCMQTKHRNYSRIKLLRTWGMDMKLRKQMSLDRIEVRPEVKEL 420

Query: 421 VVTELKEIEAIKGQEEFDQRGQWTIDRYKTLLNLNNENKLIKAIETTVSKRPFDKCIFIW 480
           VVTEL+EIE IKGQEEF+QRGQWTIDRYK  L  N+++KLIKA+ETTV+KRPFDK IFIW
Sbjct: 421 VVTELREIENIKGQEEFEQRGQWTIDRYKIKLKQNDDSKLIKALETTVNKRPFDKSIFIW 480

Query: 481 HITTNIFYNIEVHRDTSVGNKTEAIMSLSDYMMYLVVTRSHVLSTTTADIIFDHSCVKLG 540
           HITTNIFY+I    DTS+GNK EAIMS+S+YMMYL+VTRSHVLSTTT +IIFDHSCVKLG
Sbjct: 481 HITTNIFYHIRRFHDTSIGNKMEAIMSISNYMMYLLVTRSHVLSTTTGEIIFDHSCVKLG 540

Query: 541 KFTRTGRLKKEDICNDILKLKKESILHAREPHEPIESEAEKVVVGNWHLMKDVKELADCL 600
           +FTRTG    ED CN +L L+ E  L A+EPHEP  S AEK+VVGNWHL+KDVK+LA+ L
Sbjct: 541 EFTRTGHRNIEDACNKLLNLENEGTLQAKEPHEP--SGAEKIVVGNWHLLKDVKDLANSL 600

Query: 601 LTLSNENKWKLIGSMWFEMLGYAASKCEMEYHSEHIRQGGELITHVWLLIAHNVTKYSSY 660
           L LSNE+KWKLIGSMWFEMLGYAASKCEMEYHSEHIRQGGELITHVWLLIAHNVTKYSSY
Sbjct: 601 LRLSNEDKWKLIGSMWFEMLGYAASKCEMEYHSEHIRQGGELITHVWLLIAHNVTKYSSY 660

Query: 661 EYHAGGQDEETPATS 676
           EYH GGQDEE  A S
Sbjct: 661 EYHVGGQDEEIAANS 671

BLAST of Chy5G100200 vs. NCBI nr
Match: XP_004144790.1 (uncharacterized protein LOC101214084 [Cucumis sativus] >KGN61067.1 hypothetical protein Csa_021275 [Cucumis sativus])

HSP 1 Score: 1334 bits (3453), Expect = 0.0
Identity = 661/675 (97.93%), Postives = 670/675 (99.26%), Query Frame = 0

Query: 1   MFSELFDLIVPEHISFIWGYWGIELLVLANFVFQVILTFNGSRRRHTPGNKLSLIVWFSY 60
           MFSELFDLIVPEHISFIWGYWGIELLVLANFVFQVILTFNGSRRRHTPGN+LSLIVWFSY
Sbjct: 1   MFSELFDLIVPEHISFIWGYWGIELLVLANFVFQVILTFNGSRRRHTPGNRLSLIVWFSY 60

Query: 61  LLAAKIATVVLGKLTTIDIGREQRNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120
           LLAAKIATVVLGKLTTIDIG EQRNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGV
Sbjct: 61  LLAAKIATVVLGKLTTIDIGHEQRNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120

Query: 121 RQVFSMVIQVGIMFYILVRSWTDSKTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFTI 180
           RQVFSMVIQVGIMFYILVRSWTDSKTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFTI
Sbjct: 121 RQVFSMVIQVGIMFYILVRSWTDSKTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFTI 180

Query: 181 ADFFKYHEVADLFNKLPQGENELPEANLILRAYYRFCCLKPHLENWLYYPPTDCDQDKLY 240
           ADFFKYHEVADLFNKLPQGENELPEANLILRAYYRFCCLKPHLENWLYYPPTDCDQ KL+
Sbjct: 181 ADFFKYHEVADLFNKLPQGENELPEANLILRAYYRFCCLKPHLENWLYYPPTDCDQGKLH 240

Query: 241 IKECGYEDVFRITDVELGFMYDALYTKAPVVYTRKGLILRLISLLSVIATLIGFSVLFKD 300
           IKECGYEDVFRITDVELGFMYDALYTKAPVVYTRKGLILRLISLLSVIATL+GFSVLFKD
Sbjct: 241 IKECGYEDVFRITDVELGFMYDALYTKAPVVYTRKGLILRLISLLSVIATLVGFSVLFKD 300

Query: 301 AFVYNISIGFIHFVLIAALIIEIYQILRLPFTDWAIVQMIRHHEAFPILRGFLRSLSPQS 360
           AFVYNISIGFIHFVLIAALIIEIYQILRLPFTDWAIVQM+RHHEAFPILRGFLRSLSPQS
Sbjct: 301 AFVYNISIGFIHFVLIAALIIEIYQILRLPFTDWAIVQMVRHHEAFPILRGFLRSLSPQS 360

Query: 361 ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYMGMDMKLRKQLSLDRIDVHPEVKEF 420
           ATWRRWSNTMGQFNLL+FCLQTKHRNYSRIKILRYMGMDMKLRKQLSLDRIDV PEVKEF
Sbjct: 361 ATWRRWSNTMGQFNLLEFCLQTKHRNYSRIKILRYMGMDMKLRKQLSLDRIDVSPEVKEF 420

Query: 421 VVTELKEIEAIKGQEEFDQRGQWTIDRYKTLLNLNNENKLIKAIETTVSKRPFDKCIFIW 480
           VVTEL+EIEAIKG+EEFDQRGQWTI+RYKTLLNLNNENKLIKAIETTVSKRPFDKCIFIW
Sbjct: 421 VVTELREIEAIKGEEEFDQRGQWTINRYKTLLNLNNENKLIKAIETTVSKRPFDKCIFIW 480

Query: 481 HITTNIFYNIEVHRDTSVGNKTEAIMSLSDYMMYLVVTRSHVLSTTTADIIFDHSCVKLG 540
           HITTNIFYNIE +RDTSVGNKTEAIMSLSDYMMYLVVTRSHVLSTTTADIIFDHSCVKLG
Sbjct: 481 HITTNIFYNIEGYRDTSVGNKTEAIMSLSDYMMYLVVTRSHVLSTTTADIIFDHSCVKLG 540

Query: 541 KFTRTGRLKKEDICNDILKLKKESILHAREPHEPIESEAEKVVVGNWHLMKDVKELADCL 600
           KFTRTGRLKKEDICNDILKLKKESILHAREPHEPIESEAEKVVVGNWHLMKDVKELADCL
Sbjct: 541 KFTRTGRLKKEDICNDILKLKKESILHAREPHEPIESEAEKVVVGNWHLMKDVKELADCL 600

Query: 601 LTLSNENKWKLIGSMWFEMLGYAASKCEMEYHSEHIRQGGELITHVWLLIAHNVTKYSSY 660
           L LSNENKWKLIGSMWFEMLGYAASKCEMEYHSEHIRQGGELITHVWLLIAHNVTKYSSY
Sbjct: 601 LALSNENKWKLIGSMWFEMLGYAASKCEMEYHSEHIRQGGELITHVWLLIAHNVTKYSSY 660

Query: 661 EYHAGGQDEETPATS 675
           EYHAGGQDEETPATS
Sbjct: 661 EYHAGGQDEETPATS 675

BLAST of Chy5G100200 vs. NCBI nr
Match: XP_008452495.1 (PREDICTED: uncharacterized protein LOC103493508 [Cucumis melo] >XP_016901302.1 PREDICTED: uncharacterized protein LOC103493508 [Cucumis melo] >KAA0043094.1 uncharacterized protein E6C27_scaffold805G00040 [Cucumis melo var. makuwa] >TYJ96395.1 uncharacterized protein E5676_scaffold546G00210 [Cucumis melo var. makuwa])

HSP 1 Score: 1307 bits (3382), Expect = 0.0
Identity = 649/675 (96.15%), Postives = 659/675 (97.63%), Query Frame = 0

Query: 1   MFSELFDLIVPEHISFIWGYWGIELLVLANFVFQVILTFNGSRRRHTPGNKLSLIVWFSY 60
           MFSELFDLIVPEHISFIWGYWGIELLVLANFVFQVILTFNGSRRRHTPGN+LSLIVWFSY
Sbjct: 1   MFSELFDLIVPEHISFIWGYWGIELLVLANFVFQVILTFNGSRRRHTPGNRLSLIVWFSY 60

Query: 61  LLAAKIATVVLGKLTTIDIGREQRNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120
           LLAAKIATVVLGKLTTIDIGREQRNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGV
Sbjct: 61  LLAAKIATVVLGKLTTIDIGREQRNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120

Query: 121 RQVFSMVIQVGIMFYILVRSWTDSKTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFTI 180
           RQVFSMVIQVGIMFYILVRSWTDSKTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFTI
Sbjct: 121 RQVFSMVIQVGIMFYILVRSWTDSKTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFTI 180

Query: 181 ADFFKYHEVADLFNKLPQGENELPEANLILRAYYRFCCLKPHLENWLYYPPTDCDQDKLY 240
           ADFFKYHEVA+LFNKLPQGENELPEANLILRAYYRFCCLKPHLENWLYYPPTDCDQDKLY
Sbjct: 181 ADFFKYHEVANLFNKLPQGENELPEANLILRAYYRFCCLKPHLENWLYYPPTDCDQDKLY 240

Query: 241 IKECGYEDVFRITDVELGFMYDALYTKAPVVYTRKGLILRLISLLSVIATLIGFSVLFKD 300
           IK+CGYEDVFRITDVELGFMYDALYTKAPVVYTRKGLILR ISLLSVIATL GFSVLFKD
Sbjct: 241 IKDCGYEDVFRITDVELGFMYDALYTKAPVVYTRKGLILRFISLLSVIATLCGFSVLFKD 300

Query: 301 AFVYNISIGFIHFVLIAALIIEIYQILRLPFTDWAIVQMIRHHEAFPILRGFLRSLSPQS 360
           AFVYNISIGFIHFVLIAALIIEIYQILRLPFTDWAIVQM+RHHEAFPILRGFLRSL+PQS
Sbjct: 301 AFVYNISIGFIHFVLIAALIIEIYQILRLPFTDWAIVQMVRHHEAFPILRGFLRSLAPQS 360

Query: 361 ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYMGMDMKLRKQLSLDRIDVHPEVKEF 420
           ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRY GMDMKLRKQLSLDRIDVHPEV+EF
Sbjct: 361 ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYWGMDMKLRKQLSLDRIDVHPEVREF 420

Query: 421 VVTELKEIEAIKGQEEFDQRGQWTIDRYKTLLNLNNENKLIKAIETTVSKRPFDKCIFIW 480
           +VTEL+EIE IKGQEEFD RGQWTIDRYKT      ENKLIKAIETTV KRPFDKCIFIW
Sbjct: 421 LVTELREIEEIKGQEEFDNRGQWTIDRYKTKF----ENKLIKAIETTVLKRPFDKCIFIW 480

Query: 481 HITTNIFYNIEVHRDTSVGNKTEAIMSLSDYMMYLVVTRSHVLSTTTADIIFDHSCVKLG 540
           HITTNIFYNIE +RD SVGNKTEAIM LSDYMMYLVVTRSHVLSTTTADIIFDHSCVKLG
Sbjct: 481 HITTNIFYNIEGYRDNSVGNKTEAIMCLSDYMMYLVVTRSHVLSTTTADIIFDHSCVKLG 540

Query: 541 KFTRTGRLKKEDICNDILKLKKESILHAREPHEPIESEAEKVVVGNWHLMKDVKELADCL 600
           KFTRTGRLKKEDICNDIL LKKESILHAREPHEPIESEAEKVVVGNWHLMKDVKELADCL
Sbjct: 541 KFTRTGRLKKEDICNDILNLKKESILHAREPHEPIESEAEKVVVGNWHLMKDVKELADCL 600

Query: 601 LTLSNENKWKLIGSMWFEMLGYAASKCEMEYHSEHIRQGGELITHVWLLIAHNVTKYSSY 660
           LTLSNE+KWKLIGSMWFEMLGYAASKCEMEYHSEHIRQGGELITHVWLLIAHNVTKYSSY
Sbjct: 601 LTLSNEDKWKLIGSMWFEMLGYAASKCEMEYHSEHIRQGGELITHVWLLIAHNVTKYSSY 660

Query: 661 EYHAGGQDEETPATS 675
           EYHAGGQDEETPATS
Sbjct: 661 EYHAGGQDEETPATS 671

BLAST of Chy5G100200 vs. NCBI nr
Match: XP_038889477.1 (uncharacterized protein LOC120079385 [Benincasa hispida])

HSP 1 Score: 1276 bits (3302), Expect = 0.0
Identity = 628/675 (93.04%), Postives = 650/675 (96.30%), Query Frame = 0

Query: 1   MFSELFDLIVPEHISFIWGYWGIELLVLANFVFQVILTFNGSRRRHTPGNKLSLIVWFSY 60
           MFSELF+LIVP HISFIW YWGIELLVLANFVFQVILTFNGSRRRHTPGNKLSL VWFSY
Sbjct: 1   MFSELFELIVPGHISFIWSYWGIELLVLANFVFQVILTFNGSRRRHTPGNKLSLTVWFSY 60

Query: 61  LLAAKIATVVLGKLTTIDIGREQRNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120
           LLAAKIATVVLGKLTTI+IGRE+RNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGV
Sbjct: 61  LLAAKIATVVLGKLTTIEIGREKRNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120

Query: 121 RQVFSMVIQVGIMFYILVRSWTDSKTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFTI 180
           RQVFSMVIQV IMFYIL+RSWT+SKTSFLY+PMS+AGIIKYGETSWALKSALNGNFGFTI
Sbjct: 121 RQVFSMVIQVAIMFYILIRSWTNSKTSFLYIPMSVAGIIKYGETSWALKSALNGNFGFTI 180

Query: 181 ADFFKYHEVADLFNKLPQGENELPEANLILRAYYRFCCLKPHLENWLYYPPTDCDQDKLY 240
           ADFFKYHEVADLF KLPQGENELPEANLILRAYYRFCCLKPHLENWLYYPPTDCDQ+KLY
Sbjct: 181 ADFFKYHEVADLFKKLPQGENELPEANLILRAYYRFCCLKPHLENWLYYPPTDCDQEKLY 240

Query: 241 IKECGYEDVFRITDVELGFMYDALYTKAPVVYTRKGLILRLISLLSVIATLIGFSVLFKD 300
           I +C YEDVFRITD ELGFMYDALYTKAPVVYTRKGLILR ISLLSVIATL GFSVLFKD
Sbjct: 241 IDDCKYEDVFRITDSELGFMYDALYTKAPVVYTRKGLILRFISLLSVIATLCGFSVLFKD 300

Query: 301 AFVYNISIGFIHFVLIAALIIEIYQILRLPFTDWAIVQMIRHHEAFPILRGFLRSLSPQS 360
           AFVYNISIGFIHFVLIAALIIEIYQILRLPFTDWAIVQM+RHHEAFPIL GFLRSL+PQS
Sbjct: 301 AFVYNISIGFIHFVLIAALIIEIYQILRLPFTDWAIVQMVRHHEAFPILGGFLRSLAPQS 360

Query: 361 ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYMGMDMKLRKQLSLDRIDVHPEVKEF 420
           ATWRRWSNT+GQFNLLDFCLQTKHRNYSRIKILRY GMDMKLRKQLSLDRID+ PEVKE 
Sbjct: 361 ATWRRWSNTLGQFNLLDFCLQTKHRNYSRIKILRYWGMDMKLRKQLSLDRIDIPPEVKEL 420

Query: 421 VVTELKEIEAIKGQEEFDQRGQWTIDRYKTLLNLNNENKLIKAIETTVSKRPFDKCIFIW 480
           VVTEL+EIE IKGQEEFDQRGQWTIDRYK  LN NNENKLIKA+ETTVSKRPFDKCIFIW
Sbjct: 421 VVTELREIEKIKGQEEFDQRGQWTIDRYKKKLNFNNENKLIKAMETTVSKRPFDKCIFIW 480

Query: 481 HITTNIFYNIEVHRDTSVGNKTEAIMSLSDYMMYLVVTRSHVLSTTTADIIFDHSCVKLG 540
           HITTNIFYNIE +RD SVGNKTEAIMSLSDYMMYL+VTRSHVLSTTTADIIFDHSCVKLG
Sbjct: 481 HITTNIFYNIECYRDASVGNKTEAIMSLSDYMMYLLVTRSHVLSTTTADIIFDHSCVKLG 540

Query: 541 KFTRTGRLKKEDICNDILKLKKESILHAREPHEPIESEAEKVVVGNWHLMKDVKELADCL 600
           KFTRTGRLKKED+CNDIL L+KESILHAREPHEPIESEAEKVVVGNWHLMKDVKELAD L
Sbjct: 541 KFTRTGRLKKEDVCNDILNLQKESILHAREPHEPIESEAEKVVVGNWHLMKDVKELADSL 600

Query: 601 LTLSNENKWKLIGSMWFEMLGYAASKCEMEYHSEHIRQGGELITHVWLLIAHNVTKYSSY 660
           LTLSNE+KWKL+GSMWFEM+GYAASKCEMEYHSEHIRQGGELITHVWLLIAHNVTKYSSY
Sbjct: 601 LTLSNEDKWKLLGSMWFEMMGYAASKCEMEYHSEHIRQGGELITHVWLLIAHNVTKYSSY 660

Query: 661 EYHAGGQDEETPATS 675
           EYH GGQDEETP  S
Sbjct: 661 EYHVGGQDEETPPAS 675

BLAST of Chy5G100200 vs. NCBI nr
Match: XP_022999644.1 (uncharacterized protein LOC111493941 [Cucurbita maxima])

HSP 1 Score: 1169 bits (3024), Expect = 0.0
Identity = 580/675 (85.93%), Postives = 619/675 (91.70%), Query Frame = 0

Query: 1   MFSELFDLIVPEHISFIWGYWGIELLVLANFVFQVILTFNGSRRRHTPGNKLSLIVWFSY 60
           MFS+LF  I+P HISFIW  WGIELLV ANF+FQVILT+NGSRRRHTPG KLSL VWFSY
Sbjct: 1   MFSDLFLRILPGHISFIWNNWGIELLVFANFMFQVILTYNGSRRRHTPGYKLSLTVWFSY 60

Query: 61  LLAAKIATVVLGKLTTIDIGREQRNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120
           LLAAKIATVVLGKLTTIDIG E+RNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGV
Sbjct: 61  LLAAKIATVVLGKLTTIDIGHERRNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120

Query: 121 RQVFSMVIQVGIMFYILVRSWTDSKTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFTI 180
           RQVFSMVIQV IMFYILVRSWTDSKTSFLYLPMSLAGIIKYGETSWALKSAL+GN GFTI
Sbjct: 121 RQVFSMVIQVTIMFYILVRSWTDSKTSFLYLPMSLAGIIKYGETSWALKSALSGNLGFTI 180

Query: 181 ADFFKYHEVADLFNKLPQGENELPEANLILRAYYRFCCLKPHLENWLYYPPTDCDQDKLY 240
           ADFFKYHEVA+LF+KLPQ  NELPEA LILRAYYRFCCLKPHLENWLYYPPTDCD  KLY
Sbjct: 181 ADFFKYHEVANLFDKLPQ--NELPEAKLILRAYYRFCCLKPHLENWLYYPPTDCDHKKLY 240

Query: 241 IKECGYEDVFRITDVELGFMYDALYTKAPVVYTRKGLILRLISLLSVIATLIGFSVLFKD 300
           I +C YEDVFRITD ELGFMYDALYTKAPV+YTRKGLILR ISLLS+IATL GFSVLFKD
Sbjct: 241 IDDCEYEDVFRITDSELGFMYDALYTKAPVIYTRKGLILRFISLLSLIATLCGFSVLFKD 300

Query: 301 AFVYNISIGFIHFVLIAALIIEIYQILRLPFTDWAIVQMIRHHEAFPILRGFLRSLSPQS 360
           AFVYNIS+GFIH+VLIA+LIIEIYQI+R+PFTDWAIVQMIRHHE FPILRG L SL+PQS
Sbjct: 301 AFVYNISVGFIHYVLIASLIIEIYQIMRIPFTDWAIVQMIRHHETFPILRGLLHSLAPQS 360

Query: 361 ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYMGMDMKLRKQLSLDRIDVHPEVKEF 420
           ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIK+LR  G+DMKLRKQ+SLDRIDVHPEVKE 
Sbjct: 361 ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKLLRTWGLDMKLRKQMSLDRIDVHPEVKEL 420

Query: 421 VVTELKEIEAIKGQEEFDQRGQWTIDRYKTLLNLNNENKLIKAIETTVSKRPFDKCIFIW 480
           VVTEL+EIE IKGQEEF+QRGQWTIDRYKT L  N+++ LIKA+ETTV+KRPFDK IFIW
Sbjct: 421 VVTELREIEKIKGQEEFEQRGQWTIDRYKTKLKQNDDSILIKALETTVNKRPFDKSIFIW 480

Query: 481 HITTNIFYNIEVHRDTSVGNKTEAIMSLSDYMMYLVVTRSHVLSTTTADIIFDHSCVKLG 540
           HITTNIFY+I    DTS+GNK EAIMS+S+YMMYL+VTRSHVLSTTT +IIFDHSCVKLG
Sbjct: 481 HITTNIFYHIRRFHDTSIGNKMEAIMSISNYMMYLLVTRSHVLSTTTGEIIFDHSCVKLG 540

Query: 541 KFTRTGRLKKEDICNDILKLKKESILHAREPHEPIESEAEKVVVGNWHLMKDVKELADCL 600
           KFTRTG L KED CN +L L+KE  L A EPHEP  SEAEKVVVGNWHL+KDVK+LAD L
Sbjct: 541 KFTRTGHLNKEDACNALLNLQKEGTLQAEEPHEP--SEAEKVVVGNWHLLKDVKDLADSL 600

Query: 601 LTLSNENKWKLIGSMWFEMLGYAASKCEMEYHSEHIRQGGELITHVWLLIAHNVTKYSSY 660
           L LSNEN+W+LIGSMWFEMLGYAASKCEMEYHSEHIRQGGELITHVWLLIAHNVTKYSSY
Sbjct: 601 LFLSNENRWQLIGSMWFEMLGYAASKCEMEYHSEHIRQGGELITHVWLLIAHNVTKYSSY 660

Query: 661 EYHAGGQDEETPATS 675
           EYH GGQDEE  A S
Sbjct: 661 EYHVGGQDEEIAANS 671

BLAST of Chy5G100200 vs. NCBI nr
Match: KAG6599136.1 (hypothetical protein SDJN03_08914, partial [Cucurbita argyrosperma subsp. sororia] >KAG7030078.1 hypothetical protein SDJN02_08425, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1149 bits (2971), Expect = 0.0
Identity = 569/675 (84.30%), Postives = 612/675 (90.67%), Query Frame = 0

Query: 1   MFSELFDLIVPEHISFIWGYWGIELLVLANFVFQVILTFNGSRRRHTPGNKLSLIVWFSY 60
           MFS+LF  I+P HISFIW  WGIELLV ANFVFQVILT+NGSRRRHTPG KLSL VWFSY
Sbjct: 1   MFSDLFLRILPGHISFIWNNWGIELLVFANFVFQVILTYNGSRRRHTPGYKLSLTVWFSY 60

Query: 61  LLAAKIATVVLGKLTTIDIGREQRNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120
           LLAAKIATVVLGKLTTIDIG E+RNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGV
Sbjct: 61  LLAAKIATVVLGKLTTIDIGHERRNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120

Query: 121 RQVFSMVIQVGIMFYILVRSWTDSKTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFTI 180
           RQVFSM +QV IMFYILVRSWTDSKTSFLYLPMSLAGIIKYGETSWALKSAL+GN GFTI
Sbjct: 121 RQVFSMGVQVTIMFYILVRSWTDSKTSFLYLPMSLAGIIKYGETSWALKSALSGNLGFTI 180

Query: 181 ADFFKYHEVADLFNKLPQGENELPEANLILRAYYRFCCLKPHLENWLYYPPTDCDQDKLY 240
           ADFFKY EVA LF KLPQ  NELPEA LILRAYYRFCCLKPHLENWLYYPPTDCD  KLY
Sbjct: 181 ADFFKYQEVAKLFEKLPQ--NELPEAKLILRAYYRFCCLKPHLENWLYYPPTDCDHKKLY 240

Query: 241 IKECGYEDVFRITDVELGFMYDALYTKAPVVYTRKGLILRLISLLSVIATLIGFSVLFKD 300
           I +C YEDVFRITD ELGFMYDALYTKAPV+YTRKGLILR ISLLS+IATL GFSVLFKD
Sbjct: 241 IDDCEYEDVFRITDSELGFMYDALYTKAPVIYTRKGLILRFISLLSLIATLCGFSVLFKD 300

Query: 301 AFVYNISIGFIHFVLIAALIIEIYQILRLPFTDWAIVQMIRHHEAFPILRGFLRSLSPQS 360
           AFVYNIS+GFIH+VLIA+LIIE+YQI+R+PFTDWAIVQMIRHH+ FPILRG L SL+PQS
Sbjct: 301 AFVYNISVGFIHYVLIASLIIELYQIMRIPFTDWAIVQMIRHHKTFPILRGLLHSLAPQS 360

Query: 361 ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYMGMDMKLRKQLSLDRIDVHPEVKEF 420
           ATWRRWSNTMGQFNLLDFC+QTKHRNYSRIK+LR  GMDMKLRKQ+SLDRI+V PEVKE 
Sbjct: 361 ATWRRWSNTMGQFNLLDFCMQTKHRNYSRIKLLRTWGMDMKLRKQMSLDRIEVRPEVKEL 420

Query: 421 VVTELKEIEAIKGQEEFDQRGQWTIDRYKTLLNLNNENKLIKAIETTVSKRPFDKCIFIW 480
           VVTEL+EIE IKGQEEF+QRGQWTIDRYK  L  N+++KLIKA+ETTV+KRPFDK IFIW
Sbjct: 421 VVTELREIEKIKGQEEFEQRGQWTIDRYKIKLKQNDDSKLIKALETTVNKRPFDKSIFIW 480

Query: 481 HITTNIFYNIEVHRDTSVGNKTEAIMSLSDYMMYLVVTRSHVLSTTTADIIFDHSCVKLG 540
           HITTNIFY+I    DTS+GNK EAIMS+S+YMMYL+VTRSHVLSTTT +IIFDHSCVKLG
Sbjct: 481 HITTNIFYHIRRFHDTSIGNKMEAIMSISNYMMYLLVTRSHVLSTTTGEIIFDHSCVKLG 540

Query: 541 KFTRTGRLKKEDICNDILKLKKESILHAREPHEPIESEAEKVVVGNWHLMKDVKELADCL 600
           +FTRTG   KED CN +L L+ E  L A+EPHEP  S AEK+VVGNWHL+KDVK+LA+ L
Sbjct: 541 EFTRTGHRNKEDACNKLLNLENEGTLQAKEPHEP--SGAEKIVVGNWHLLKDVKDLANSL 600

Query: 601 LTLSNENKWKLIGSMWFEMLGYAASKCEMEYHSEHIRQGGELITHVWLLIAHNVTKYSSY 660
           L LSNE+KWKLIGSMWFEMLGYAASKCEMEYHSEHIRQGGELITHVWLLIAHNVTKYSSY
Sbjct: 601 LRLSNEDKWKLIGSMWFEMLGYAASKCEMEYHSEHIRQGGELITHVWLLIAHNVTKYSSY 660

Query: 661 EYHAGGQDEETPATS 675
           EYH GGQDEE  A S
Sbjct: 661 EYHVGGQDEEIAANS 671

BLAST of Chy5G100200 vs. TAIR 10
Match: AT5G45540.1 (Protein of unknown function (DUF594) )

HSP 1 Score: 189.9 bits (481), Expect = 6.7e-48
Identity = 190/791 (24.02%), Postives = 336/791 (42.48%), Query Frame = 0

Query: 9   IVPEHISFIWGYWGIELLVLANFVFQVILTFNGSRRRHTPGNKLSLIVWFSYLLAAKIAT 68
           ++P H+  +W  W I  +++ +   Q IL F    RR T      +++W +YLLA   A 
Sbjct: 4   MIPPHLRKLWDKWNIRGVIILSLFLQTILIFFAPSRRRTAKKLFLVLIWSAYLLADWAAD 63

Query: 69  VVLGKLTTIDIGREQRNTHTQVQALLA---PLMFMQIGNPDTITAYSIEDNQLGVRQVFS 128
             +G+++       + N  ++ + LLA   P + + +G PDTITA ++EDN+L  R +FS
Sbjct: 64  YAVGQISDSQEEEAESNKPSKNRELLAFWSPFLLLHLGGPDTITALALEDNELWDRHLFS 123

Query: 129 MVIQVGIMFYILVRSWTDSKTSFLYLPMSLAGIIKYGETSWALKSALNGNF--------- 188
           +V Q     Y+++ S   ++     L M + G+IKY E + AL SA    F         
Sbjct: 124 LVCQAVATVYVILLS-IPNRLLTPTLIMFVGGVIKYVERTAALFSASLDKFKDSMLDDPD 183

Query: 189 -GFTIADFFKYHEVADLFN--------KLPQ----------GENELPEANLILRAYYRFC 248
            G   A   + +E     N        K P+           +NEL    +I  AY  F 
Sbjct: 184 PGANYAKLMEEYEARKKMNMPTDVIVVKDPEKGREGNTPVRPDNELTALQVIQYAYKYFN 243

Query: 249 CLKPHLENWLYYPPTDCDQDKLYIKECGYEDVFRITDVELGFMYDALYTKAPVVYTRKGL 308
             K  + + L +   + D+ + +  +   E+  RI +VELG +YD L+TKA +++   G 
Sbjct: 244 IFKGLIVD-LIFTNQERDESRKFFDKLTAEEALRIIEVELGLIYDCLFTKAEILHNWTGA 303

Query: 309 ILRLISLLSVIATLIGFSVLFKDAFVYNISIGFIHFVLIAALIIEIYQILRLPFTDWAIV 368
           + R I+L  ++A+L  F +  KD +     +   + +LI  + ++   +L    +DW I 
Sbjct: 304 VFRFIALGCLVASLCLFKMNKKDQY-DGFDVVLTYALLICGIALDSIALLMFCVSDWTIA 363

Query: 369 QMIRHHE-----------------AFPILRGFLRSLSPQSA--------TWRRWSNTMGQ 428
           ++ +  E                  F  LR + RS   Q           +RRWS  +  
Sbjct: 364 RLRKLKEDLEEKDTLTDRVLNWILDFKTLR-WKRSKCSQDGHQVLNRNFMFRRWSEYVHA 423

Query: 429 FNLLDFCL--QTKHRNYSRIKI-------------------------------------- 488
           +NL+ FCL  + K  +Y++ KI                                      
Sbjct: 424 YNLIGFCLGIRPKRIHYTKGKIHSFFHQTVHILSIDTAIENATRGTRQFHNWIGRFLSNL 483

Query: 489 -----------LRYMGMDMKLRKQLSLDRID------------------VHPEVKEFVVT 548
                      LR+     +L   L  + +D                  +  E+ EF+ T
Sbjct: 484 SKRDNSVIRTGLRWFLFFPQLLGLLIYNFLDFFGIKDLVEEIRFTVSDRLTRELWEFIFT 543

Query: 549 ELKEIEAIKGQEE-----FDQRGQWTI--DRYKTLLNLNNENKLIKAIETTVSKRPFDKC 608
           E+++       +E        RG WT+     K   +  +  KL++     V+++ +D+ 
Sbjct: 544 EVQQKHRFAEDQESAKGISSARGNWTLLETSSKKKEDGTDHTKLLQ----YVTEKDYDQS 603

Query: 609 IFIWHITTNIFYNIEV------------HRDTSVGNKTEAIMSLSDYMMYLVVTRSHVLS 653
           I +WHI T + Y   +            +R+    +  E    LSDYMMYL++ +  ++S
Sbjct: 604 ILLWHIATELLYQKPIDKKVTEKEEHSTNREKEEHSNREFSKILSDYMMYLLIVQPTLMS 663

BLAST of Chy5G100200 vs. TAIR 10
Match: AT5G45530.1 (Protein of unknown function (DUF594) )

HSP 1 Score: 154.1 bits (388), Expect = 4.1e-37
Identity = 185/797 (23.21%), Postives = 328/797 (41.15%), Query Frame = 0

Query: 9   IVPEHISFIWGYWGIELLVLANFVFQVILTFNGSRRRHTPGNKLSLIVWFSYLLAAKIAT 68
           ++P  I  I   W I  LV+ + +FQ  L F    R+ T    L+ ++W +YLLA   A 
Sbjct: 3   VIPPAIKKILDKWNIRGLVIMSLLFQTSLIFLAPMRKRTSKKLLAAVLWTAYLLADWTAN 62

Query: 69  VVLGKLTTIDIGREQR----NTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGVRQVF 128
             + ++T  + G+E        + ++ AL AP + + +G PDTITA ++EDN L  R +F
Sbjct: 63  YAVSQITK-NQGKETEPGDPPKNKKLLALWAPFLLLHLGGPDTITALALEDNALWQRHLF 122

Query: 129 SMVIQVGIMFYILVRSWTDSKTSFLYLPMSL---AGIIKYGETSWALKSALNGNF----- 188
            +V Q     Y +V+    S  + L+ P++L    G IKY E + AL SA    F     
Sbjct: 123 GLVSQALAGVYAVVQ----SLENVLWPPITLLFITGTIKYVERTRALYSASLDKFKDRML 182

Query: 189 -----GFTIADFFKYHEVADLFN------------------KLPQGENELPEANLILRAY 248
                G   A   +      + N                   L + + +L +  ++   +
Sbjct: 183 QRADAGSNYAKLMEEFASRKMSNLPTEIFLTDEPDKHERPPTLVKPDRDLTDLEIVQYGF 242

Query: 249 YRFCCLKPHLENWLYYPPTDCDQDKLYIKECGYEDVFRITDVELGFMYDALYTKAPVVYT 308
             F   K  + + L +   + D+ + + KE    +  RI + ELGF+Y+++YTK  +++T
Sbjct: 243 KFFNTFKGLVVD-LIFSFRERDESRDFFKELKPGEALRIIETELGFLYESMYTKTAILHT 302

Query: 309 RKGLILRLISLLSVIATLIGFSVL-FKDAFVYNISIGFIHFVLIAALIIEIYQILRLPFT 368
             G + RLIS  S++++   F     K    +   +   + + I  + +++  ++    +
Sbjct: 303 GIGTLFRLISFGSLLSSFFVFHRRPLKSEDFHGADVVITYVLFIVGIALDLASMVIFLLS 362

Query: 369 DWAIVQMIRHHEAFP---------ILRGFLRSLSPQ-----------------SATWRRW 428
           DW    ++R+ +  P         +   FL    P+                     RRW
Sbjct: 363 DWTFA-VLRNLKDDPEEKSTSIDSLFNWFLEFRKPRWKKHTCNGNQTHEVLSTGFFTRRW 422

Query: 429 SNTMGQFNLLDFCLQTKHRNYSRIKILRYMGM----------DMKLR------------- 488
           S T+  FN + FCL+ K    SRI   R   +          D+ +R             
Sbjct: 423 SGTIYGFNFIGFCLKAK---VSRIHQKRNCNLLVWDYVVSLFDLVIRRIQMMIGWIKNVN 482

Query: 489 -----------KQLSLDRIDVHP------------------------------------- 548
                      K+  + R  V+P                                     
Sbjct: 483 RSIRSVLRQWSKKNPMIRCTVYPLYLVFFAGIPEVFRVLWKYIDRIFSVTSYLDGIRFIS 542

Query: 549 ------EVKEFVVTELKEIEAIKGQEEFDQ-----RGQWTIDRYKTLLNLNNENKLIKAI 608
                    EF+  E+K+        E  +     RG+W +   K    L   + L++ I
Sbjct: 543 REPLTKNQWEFIFNEVKDKSGFAETPEVAKKVSWARGEWALRDSK----LMEVDTLMRYI 602

Query: 609 ETTVSKRPFDKCIFIWHITTNIFYNIE----VHRDTSVG-NKTEAIMSLSDYMMYLVVTR 653
           E    K  +D+ + +WHI T + +  E    + + +  G +  E    +SDYMMYL++ R
Sbjct: 603 E----KVDYDQSLLLWHIATELCFQKEEGGKMEKLSREGYDDREFSKIISDYMMYLLIMR 662

BLAST of Chy5G100200 vs. TAIR 10
Match: AT5G45470.1 (Protein of unknown function (DUF594) )

HSP 1 Score: 145.6 bits (366), Expect = 1.5e-34
Identity = 196/854 (22.95%), Postives = 327/854 (38.29%), Query Frame = 0

Query: 9   IVPEHISFIWGYWGIELLVLANFVFQVILTFNGSRRRHTPGNKLSLIVWFSYLLA---AK 68
           ++P+HI  +W  W I   V+ +   Q IL      R+ TP   L ++VW SYLLA   A 
Sbjct: 4   VIPKHIKDVWDRWNIRGAVILSLTLQAILICFSPLRKRTPRRLLIVLVWSSYLLADWSAN 63

Query: 69  IATVVLGKLTTIDIGREQRNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGVRQVFS 128
            A  ++ K    D+  +      +V AL AP + + +G PDTITA+++EDN L +R VF 
Sbjct: 64  FAVGLISKNQGKDLKPDDPPQDKKVMALWAPFLLLHLGGPDTITAFALEDNALWLRHVFG 123

Query: 129 MVIQVGIMFYILVRSWTDSKTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFT------ 188
           +V Q     Y++V S  +S    + L + ++G IKY E + AL SA    F  +      
Sbjct: 124 LVFQAIAGVYVVVMSLPNSLWVVIVL-VFVSGTIKYLERTTALYSASLDKFRDSMIQAPD 183

Query: 189 -------IADFFKYHEVADLFNKL-----PQGEN-----------------ELPEANLIL 248
                  + + +K  + A L  K+     P  EN                 +L +  ++ 
Sbjct: 184 PGPNYAKLMEEYKAKKEARLPTKIVLIDEPDKENRPKKLEHPALASKKRKKDLTDLEIVQ 243

Query: 249 RAYYRFCCLKPHLENWLYYPPTDCDQDKLYIKECGYEDVFRITDVELGFMYDALYTKAPV 308
            AY  F   K  + N ++      +  +++      E+  RI ++ELGF+YDAL+TK  +
Sbjct: 244 YAYKFFNTFKGLVVNLIFSFRERDESLEIFENLNDPEEALRIIEIELGFLYDALFTKIAI 303

Query: 309 VYTRKGLILRLISLLSVIATLIGF-SVLFKDAFVYNISIGFIHFVLIAALIIEIYQILRL 368
           ++T  G + R+ +  +++A  I F     K    +   +   + +    L+++   IL  
Sbjct: 304 LHTGIGTVSRVFASGTLVAAFIIFHKKPNKGTDFHGADVVVTYTLFAVGLVLDFISILLF 363

Query: 369 PFTDW--AIVQMIR---------HHEAFPILRGFLR-SLSPQSA---------------- 428
            F+DW  A    ++             F  L  F +   +PQ                  
Sbjct: 364 LFSDWTCAAYSSLKDDPDELQSWQERCFNWLLKFRKLRWTPQECHKTGMHKCTKEGLKPC 423

Query: 429 ---------------------------------------------TWRRWSNTMGQFNLL 488
                                                          RRWS ++  FN +
Sbjct: 424 IMKGADKKEGGNKNEGGNKKEGDDKKEAADKCSLVDKHDVLTTRFFLRRWSGSINVFNFI 483

Query: 489 DFCLQT----------KHRNYS-----------RIKILRYMGMDMKL------------- 548
            +  +             R YS              I +  G  +KL             
Sbjct: 484 AYATKADVERIHDARGSFRRYSWKIITFPFKKLNFVIKKIFGSIVKLINEVHRRISHEVN 543

Query: 549 ---RKQL------------------------------------SLDRID----VH----- 608
              RK L                                    +LD +     VH     
Sbjct: 544 ALSRKHLWARRFLYPIYFEFISRIPHFIKSVWDILSEFFDISDTLDMVHKTLFVHGEPMT 603

Query: 609 PEVKEFVVTELKEIEAIKGQEEFDQ-----RGQWTIDRYKTLLNLNNENKLIKAIETTVS 653
            E+ +F+  ELK         E  +     RG+WT+ R    ++   E KL++     V+
Sbjct: 604 RELWKFIFEELKNKSKYGDSPENAKRISLARGEWTL-RENLPVDAERE-KLVR----YVT 663

BLAST of Chy5G100200 vs. TAIR 10
Match: AT5G45460.1 (unknown protein; BEST Arabidopsis thaliana protein match is: Protein of unknown function (DUF594) (TAIR:AT5G45470.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 120.9 bits (302), Expect = 3.8e-27
Identity = 107/416 (25.72%), Postives = 187/416 (44.95%), Query Frame = 0

Query: 9   IVPEHISFIWGYWGIELLVLANFVFQVILTFNGSRRRHTPGNKLSLIVWFSYLLA---AK 68
           ++P+HI   W  W I   +  +   Q  L      R+ TP   L +++W SYLLA   A 
Sbjct: 4   VIPKHIKDAWDRWNIRGTIFLSLTLQAFLICFSPLRKRTPRRHLIIVIWSSYLLADWSAN 63

Query: 69  IATVVLGKLTTIDIGREQRNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGVRQVFS 128
            A  ++ K    D+  +      ++ AL AP + + +G PDTITA+++EDN L +R VF 
Sbjct: 64  FAVGLISKNQGKDLKPDDPPQDKKLMALWAPFLLLHLGGPDTITAFALEDNALWLRNVFG 123

Query: 129 MVIQVGIMFYILVRSWTDSKTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFT------ 188
           +V Q     Y++++S  +S    + L + ++G IKY E + AL SA    F  +      
Sbjct: 124 LVFQAIAGVYVVLQSLPNSLWVTILL-VFISGTIKYLERTTALYSASLDKFRDSMIQGPD 183

Query: 189 -------IADFFKYHEVADLFNKL-----PQGEN-----------------ELPEANLIL 248
                  + + +K  + A L  K+     P  E+                 EL    +  
Sbjct: 184 PGPNYAKLMEEYKAKKEAKLPTKIILIDEPDKEHRPKKLEHPSLASETKRKELTHLEIAQ 243

Query: 249 RAYYRFCCLKPHLENWLYYPPTDCDQDKLYIKECGYEDVFRITDVELGFMYDALYTKAPV 308
            AY  F   K  + N ++         +++      E+  RI ++ELGF+YDAL+TK  V
Sbjct: 244 YAYKFFNTFKGLVVNLIFSFRERDQSIEIFQNLEDPEEALRIIEIELGFLYDALFTKNAV 303

Query: 309 VYTRKGLILRLISLLSVIATLIGFSVLFKDAFVYNISIGFIHFVLIA-ALIIEIYQILRL 368
           ++T  G + R+++  S++A  I F  +      ++ +   I ++L A  L+++   IL  
Sbjct: 304 LHTVLGTVSRVVASGSLVAAFIIFHKISNKGRDFHGADVVITYILFAVGLVLDFISILLF 363

Query: 369 PFTDWAIVQMIRHHEAFPILRGFLRSLSPQSATWR-RWSNTMGQFNLLDFCLQTKH 385
            F+DW    +             L+    +  +W+ R+ N + +F  L + +Q  H
Sbjct: 364 LFSDWTCAAL-----------SSLKDDPDEPLSWKDRFFNCLLEFRKLRWKMQECH 407

BLAST of Chy5G100200 vs. TAIR 10
Match: AT4G19090.1 (Protein of unknown function (DUF594) )

HSP 1 Score: 99.8 bits (247), Expect = 9.2e-21
Identity = 152/690 (22.03%), Postives = 265/690 (38.41%), Query Frame = 0

Query: 92  ALLAPLMFMQIGNPDTITAYSIEDNQLGVRQVFSMVIQVGIMFYILVRSWTDSKTSFLYL 151
           AL AP + + +G PDTITA+S+EDN L  R    +V Q     Y++V+S   +  S + L
Sbjct: 84  ALWAPFLLLHLGGPDTITAFSLEDNALWNRHFLGLVFQALAGVYVVVQS-LPNVLSVIIL 143

Query: 152 PMSLAGIIKYGETSWALKSALNGNFGFTIA-------DFFKYHEVADLFNKLPQGEN--- 211
            + +AG  KY E + AL  A +  +  ++        D+       D+  KL    N   
Sbjct: 144 LLFIAGTSKYLERTIALYLASSDKYRNSMLQASNSRFDYTDQTRDLDMDTKLASEMNMKE 203

Query: 212 --ELPEANLILRAYYRFCCLKPHLENWLYYPPTDC-DQDKLYIKECGYED-VFRITDVEL 271
               P+   +L+ +        HLE   Y    +  D+ K +      +D  F I + EL
Sbjct: 204 HRGQPKPLKLLQPHKEL----THLEILQYAFFLELRDESKAFFSALQLKDEAFCIIEAEL 263

Query: 272 GFMYDALYTKAPVVYTRKGLILRLISLLSVIATLIGFSVLF-KDAFVYNISIGFIHFVLI 331
            F+Y+ LYTK  V+++  GL+ R ISL S+++    +     K    +   I   + + +
Sbjct: 264 DFIYEGLYTKGSVLHSWVGLVSRFISLGSLLSAFTIYHYRHNKIQEFHKADIVITYTLFL 323

Query: 332 AALIIEIYQILRLPFTDW--AIVQMIR-----HHEAFPILRGFLRSLSPQSATW------ 391
             + +++  I     +DW  AI+  ++      +     +  ++  L      W      
Sbjct: 324 VGIALDVISIHMFMVSDWTTAILAKLKDDPDERYSGKDHILNWILFLKRPKWKWQTCREG 383

Query: 392 ------------RRWSNTMGQFNLLDF--------------------------------- 451
                       RRW+ ++   N L +                                 
Sbjct: 384 DQQEVLNTPFLLRRWTGSITMLNFLTYSMKADTERIHDPRSRACQLLWKIAVCPFSFIFS 443

Query: 452 CLQTKHRNYSR-----------IKILRYMGMDMKLRKQLSLDRIDVH-----PEVKEFVV 511
            +QT   N ++             I+   G +   R  + + +  V      P    ++V
Sbjct: 444 IIQTWFSNIAKWFCALHWYIIHHVIILPPGQNQVARSIIGIFKAFVEFWFNVPNFFVYLV 503

Query: 512 TELKEIEAIKGQEEFDQRGQWTIDRYKTLLNLNNENKLIKAIETTVSKR----------- 571
           T +     I              D +  +  L   ++++ ++E T +K            
Sbjct: 504 TSVINFLGIN-------------DLFYEIRLLQKNSQILYSLENTTTKSSARRDWASSHT 563

Query: 572 -----------------PFDKCIFIWHITTNIFYNIE----VHRDTSVGNKTEAIMS-LS 631
                             ++  + IWHI T + Y  E     + D S  +    I   +S
Sbjct: 564 QIQIADHDMLLHYITDVDYEHSLLIWHIATELCYQEEDSAKENCDKSEYHTNRKISKIIS 623

Query: 632 DYMMYLVVTRSHVLSTTT--ADIIFDHSCVKLGKFTRTGRLKKEDICNDI--LKLKKESI 653
           DYMMYL++ +  ++S       I F  +  +  +F      KK  I  D   +KL  + I
Sbjct: 624 DYMMYLLIMQPKLMSEVAGIGKIRFRDTLAEADRF-----FKKMGIIRDSRNVKLASKEI 683

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0LK450.0e+0097.93DUF4220 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G036650 PE=... [more]
A0A1S4DZ970.0e+0096.15uncharacterized protein LOC103493508 OS=Cucumis melo OX=3656 GN=LOC103493508 PE=... [more]
A0A5A7TID10.0e+0096.15DUF4220 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A6J1KHN90.0e+0085.93uncharacterized protein LOC111493941 OS=Cucurbita maxima OX=3661 GN=LOC111493941... [more]
A0A6J1G3A80.0e+0084.15uncharacterized protein LOC111450396 OS=Cucurbita moschata OX=3662 GN=LOC1114503... [more]
Match NameE-valueIdentityDescription
XP_004144790.10.097.93uncharacterized protein LOC101214084 [Cucumis sativus] >KGN61067.1 hypothetical ... [more]
XP_008452495.10.096.15PREDICTED: uncharacterized protein LOC103493508 [Cucumis melo] >XP_016901302.1 P... [more]
XP_038889477.10.093.04uncharacterized protein LOC120079385 [Benincasa hispida][more]
XP_022999644.10.085.93uncharacterized protein LOC111493941 [Cucurbita maxima][more]
KAG6599136.10.084.30hypothetical protein SDJN03_08914, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
AT5G45540.16.7e-4824.02Protein of unknown function (DUF594) [more]
AT5G45530.14.1e-3723.21Protein of unknown function (DUF594) [more]
AT5G45470.11.5e-3422.95Protein of unknown function (DUF594) [more]
AT5G45460.13.8e-2725.72unknown protein; BEST Arabidopsis thaliana protein match is: Protein of unknown ... [more]
AT4G19090.19.2e-2122.03Protein of unknown function (DUF594) [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007658Protein of unknown function DUF594PFAMPF04578DUF594coord: 605..652
e-value: 2.1E-18
score: 65.7
IPR025315Domain of unknown function DUF4220PFAMPF13968DUF4220coord: 57..377
e-value: 7.5E-53
score: 180.1
NoneNo IPR availablePANTHERPTHR31325:SF221DUF594 FAMILY PROTEINcoord: 36..645
NoneNo IPR availablePANTHERPTHR31325OS01G0798800 PROTEIN-RELATEDcoord: 36..645

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy5G100200.1Chy5G100200.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane