Chy5G099740 (gene) Cucumber (hystrix) v1

BLAST of Chy5G099740 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 496.9 bits (1278), Expect = 2.7e-139
Identity = 250/502 (49.80%), Postives = 341/502 (67.93%), Query Frame = 0

Query: 1   MKFVQDASDFPIKEEYDYIIVGGGTTGCPLAATLSKKFSVLLIERGSEPSKYPSVLNEQQ 60
           ++F  DA+D  ++  YDY+IVGGGT+GCPLAATLS+K+ VL++ERGS P+ YP+VL    
Sbjct: 39  LRFAYDATDLELEGSYDYVIVGGGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVLTADG 98

Query: 61  LLNVFTVEDDGKNPFNRFISEDGVENIRGRVLGGSSMINGGVYSRADPEFFRTQLGMQEL 120
            +     EDDGK P  RF+SEDG++N+RGRVLGG+SMIN GVY+RA+   +    G+ + 
Sbjct: 99  FVYNLQQEDDGKTPVERFVSEDGIDNVRGRVLGGTSMINAGVYARANTSIYSAS-GV-DW 158

Query: 121 DMEMVEKAYEWVEEAIVFKPSLNLWQGAFRRFLVEGGVEPDNGFDLRDDVGTKISGSIFD 180
           DM++V K YEWVE+ IVFKP+   WQ       +E GV+P++GF L  + GT+I+GS FD
Sbjct: 159 DMDLVNKTYEWVEDTIVFKPNYQPWQSVTGTAFLEAGVDPNHGFSLDHEAGTRITGSTFD 218

Query: 181 ENGTRHGAVELLNKAQPTNLKVAVQAIVQRILFS---GLSANGVLYSDSKGKLHTAIIRK 240
             GTRH A ELLNK    NL+V V A V++I+FS   GL+A GV+Y DS G  H A +R 
Sbjct: 219 NKGTRHAADELLNKGNSNNLRVGVHASVEKIIFSNAPGLTATGVIYRDSNGTPHRAFVRS 278

Query: 241 KGEIIVSAGAIGSPQLLLLSGIGPKSYLSSLKLPVVLHQPHVGQSMVDYPRFGYVLAWPF 300
           KGE+IVSAG IG+PQLLLLSG+GP+SYLSSL +PVVL  P+VGQ + D PR    +  P 
Sbjct: 279 KGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNIPVVLSHPYVGQFLHDNPRNFINILPPN 338

Query: 301 PLAFTSSKVIGISQNKTFYFQSIASTTPLSIPPLFSIFPPNSTSLTTTSLATIGGKFSKV 360
           P+  T   V+GIS +   ++Q   S+ P + PP FS FP  S  L  ++ A    K +  
Sbjct: 339 PIEPTIVTVLGISND---FYQCSFSSLPFTTPP-FSFFPSTSYPLPNSTFAHFASKVAGP 398

Query: 361 ASTGSLRLNSSTDAASNPIVRFNYYSHPADVAMCVKGVRKVGDFLKTQTVENIKTRDLEG 420
            S GSL L SS++   +P V+FNYYS+P D++ CV G++K+G+ L T  ++  K  DL G
Sbjct: 399 LSYGSLTLKSSSNVRVSPNVKFNYYSNPTDLSHCVSGMKKIGELLSTDALKPYKVEDLPG 458

Query: 421 NKTIQFVGLPLPGNLSDDSAVGEFCKKTVTSYWHYHGGCLVGKVVNGNYSVIGVKNLRVL 480
            +    +G+PLP + +DD+A   FC+++V SYWHYHGGCLVGKV++G++ V G+  LRV+
Sbjct: 459 IEGFNILGIPLPKDQTDDAAFETFCRESVASYWHYHGGCLVGKVLDGDFRVTGIDALRVV 518

Query: 481 DGSTFAVSPGSNPTATLMMLAR 500
           DGSTF  +P S+P    +ML R
Sbjct: 519 DGSTFPYTPASHPQGFYLMLGR 534

BLAST of Chy5G099740 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 4.7e-136
Identity = 245/502 (48.80%), Postives = 338/502 (67.33%), Query Frame = 0

Query: 1   MKFVQDASDFPIKEEYDYIIVGGGTTGCPLAATLSKKFSVLLIERGSEPSKYPSVLNEQQ 60
           + F  DA+D  ++  YDY+IVGGGT+GCPLAATLS+K+ VL++ERGS P+ YP+VL    
Sbjct: 39  LSFAYDATDLELEGSYDYVIVGGGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVLTADG 98

Query: 61  LLNVFTVEDDGKNPFNRFISEDGVENIRGRVLGGSSMINGGVYSRADPEFFRTQLGMQEL 120
            +     EDDGK P  RF+SEDG++N+RGRVLGG+S+IN GVY+RA+   +    G+ + 
Sbjct: 99  FVYNLQQEDDGKTPVERFVSEDGIDNVRGRVLGGTSIINAGVYARANTSIYSAS-GV-DW 158

Query: 121 DMEMVEKAYEWVEEAIVFKPSLNLWQGAFRRFLVEGGVEPDNGFDLRDDVGTKISGSIFD 180
           DM++V + YEWVE+ IV+KP+   WQ   +   +E GV P++GF L  + GT+I+GS FD
Sbjct: 159 DMDLVNQTYEWVEDTIVYKPNSQSWQSVTKTAFLEAGVHPNHGFSLDHEEGTRITGSTFD 218

Query: 181 ENGTRHGAVELLNKAQPTNLKVAVQAIVQRILFS---GLSANGVLYSDSKGKLHTAIIRK 240
             GTRH A ELLNK    NL+V V A V++I+FS   GL+A GV+Y DS G  H A +R 
Sbjct: 219 NKGTRHAADELLNKGNSNNLRVGVHASVEKIIFSNAPGLTATGVIYRDSNGTPHQAFVRS 278

Query: 241 KGEIIVSAGAIGSPQLLLLSGIGPKSYLSSLKLPVVLHQPHVGQSMVDYPRFGYVLAWPF 300
           KGE+IVSAG IG+PQLLLLSG+GP+SYLSSL +PVVL  P+VGQ + D PR    +  P 
Sbjct: 279 KGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNIPVVLSHPYVGQFLHDNPRNFINILPPN 338

Query: 301 PLAFTSSKVIGISQNKTFYFQSIASTTPLSIPPLFSIFPPNSTSLTTTSLATIGGKFSKV 360
           P+  T   V+GIS +   ++Q   S+ P + PP F  FP  S  L  ++ A    K +  
Sbjct: 339 PIEPTIVTVLGISND---FYQCSFSSLPFTTPP-FGFFPSASYPLPNSTFAHFASKVAGP 398

Query: 361 ASTGSLRLNSSTDAASNPIVRFNYYSHPADVAMCVKGVRKVGDFLKTQTVENIKTRDLEG 420
            S GSL L SS++   +P V+FNYYS+  D++ CV G++K+G+ L T  ++  K  DL G
Sbjct: 399 LSYGSLTLKSSSNVRVSPNVKFNYYSNLTDLSHCVSGMKKIGELLSTDALKPYKVEDLPG 458

Query: 421 NKTIQFVGLPLPGNLSDDSAVGEFCKKTVTSYWHYHGGCLVGKVVNGNYSVIGVKNLRVL 480
            +    +G+PLP + +DD+A   FC+++V SYWHYHGGCLVGKV++G++ V G+  LRV+
Sbjct: 459 VEGFNILGIPLPKDQTDDAAFETFCRESVASYWHYHGGCLVGKVLDGDFRVTGINALRVV 518

Query: 481 DGSTFAVSPGSNPTATLMMLAR 500
           DGSTF  +P S+P    +ML R
Sbjct: 519 DGSTFPYTPASHPQGFYLMLGR 534

BLAST of Chy5G099740 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 2.3e-135
Identity = 248/503 (49.30%), Postives = 336/503 (66.80%), Query Frame = 0

Query: 1   MKFVQDASDFPIKEEYDYIIVGGGTTGCPLAATLSKKFSVLLIERGSEPSKYPSVLNEQQ 60
           + FV DA+D  ++  YDYIIVGGGT GCPLAATLS  +SVL++ERGS P++YP++L    
Sbjct: 39  LSFVYDATDPELEGSYDYIIVGGGTAGCPLAATLSANYSVLVLERGSLPTEYPNLLISDG 98

Query: 61  LLNVFTVEDDGKNPFNRFISEDGVENIRGRVLGGSSMINGGVYSRADPEFFRTQLGMQEL 120
            +     EDDGK P  RF+SEDG++N+RGRVLGG+SMIN GVY RA+  FF  Q G+ E 
Sbjct: 99  FVYNLQQEDDGKTPVERFVSEDGIDNVRGRVLGGTSMINAGVYVRANTSFF-NQTGI-EW 158

Query: 121 DMEMVEKAYEWVEEAIVFKPSLNLWQGAFRRFLVEGGVEPDNGFDLRDDVGTKISGSIFD 180
           DM++V + YEWVE+ IVF+P    WQ       +E G+ P+NGF +    GT+++GS FD
Sbjct: 159 DMDLVNQTYEWVEDTIVFEPDSQTWQTVIGTAYLEAGILPNNGFSVDHLAGTRLTGSTFD 218

Query: 181 ENGTRHGAVELLNKAQPTNLKVAVQAIVQRILF----SGLSANGVLYSDSKGKLHTAIIR 240
            NGTRH + ELLNK  P NL+VAVQA V++I+F    SG++A GV+Y+DS G  H A +R
Sbjct: 219 NNGTRHASDELLNKGDPNNLRVAVQAAVEKIIFSSNTSGVTAIGVIYTDSNGTTHQAFVR 278

Query: 241 KKGEIIVSAGAIGSPQLLLLSGIGPKSYLSSLKLPVVLHQPHVGQSMVDYPRFGYVLAWP 300
            +GE+I+SAG IGSPQLLLLSG+GP+SYL+SL + VV   P+VGQ + D PR    +  P
Sbjct: 279 GEGEVILSAGPIGSPQLLLLSGVGPESYLTSLNISVVASHPYVGQYIYDNPRNFINILPP 338

Query: 301 FPLAFTSSKVIGISQNKTFYFQSIASTTPLSIPPLFSIFPPNSTSLTTTSLATIGGKFSK 360
            P+  ++  V+GI+ +   ++Q   S+ P   PP FS FP  S  L   + A I  K   
Sbjct: 339 NPIEASTVTVLGITSD---FYQCSISSLPFDTPP-FSFFPTTSYPLPNQTFAHIVNKVPG 398

Query: 361 VASTGSLRLNSSTDAASNPIVRFNYYSHPADVAMCVKGVRKVGDFLKTQTVENIKTRDLE 420
             S G++ LNSS+D    P V+FNYYS+  D++ CV G++K+G+ L T  +E  K  DL 
Sbjct: 399 PLSHGTVTLNSSSDVRVGPNVKFNYYSNLTDLSHCVSGMKKLGEVLSTDALEPYKVEDLP 458

Query: 421 GNKTIQFVGLPLPGNLSDDSAVGEFCKKTVTSYWHYHGGCLVGKVVNGNYSVIGVKNLRV 480
           G      +G+PLP N +DD+A   FC+++V SYWHYHGGCLVGKV++  + V G+  LRV
Sbjct: 459 GIDGFNILGIPLPENQTDDAAFETFCRESVASYWHYHGGCLVGKVLDDGFRVTGINALRV 518

Query: 481 LDGSTFAVSPGSNPTATLMMLAR 500
           +DGSTF  +P S+P    +ML R
Sbjct: 519 VDGSTFPSTPASHPQGFYLMLGR 535

BLAST of Chy5G099740 vs. ExPASy Swiss-Prot
Match: O24243 ((R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 477.2 bits (1227), Expect = 2.2e-133
Identity = 246/503 (48.91%), Postives = 338/503 (67.20%), Query Frame = 0

Query: 1   MKFVQDASDFPIKEEYDYIIVGGGTTGCPLAATLSKKFSVLLIERGSEPSKYPSVLNEQQ 60
           +KFV +A+D  ++  YDYI++GGGT+GCPLAATLS+K+ VLL+ERG+  ++YP+ L    
Sbjct: 39  LKFVYNATDTSLEGSYDYIVIGGGTSGCPLAATLSEKYKVLLLERGTIATEYPNTLTADG 98

Query: 61  LLNVFTVEDDGKNPFNRFISEDGVENIRGRVLGGSSMINGGVYSRADPEFFRTQLGMQEL 120
                  +DDGK P  RF+SEDG++N+R R+LGG+++IN GVY+RA+  F+ +Q G+ E 
Sbjct: 99  FAYNLQQQDDGKTPVERFVSEDGIDNVRARILGGTTIINAGVYARANISFY-SQTGI-EW 158

Query: 121 DMEMVEKAYEWVEEAIVFKPSLNLWQGAFRRFLVEGGVEPDNGFDLRDDVGTKISGSIFD 180
           D+++V K YEWVE+AIV KP+   WQ       +E G+ PDNGF L  + GT+++GS FD
Sbjct: 159 DLDLVNKTYEWVEDAIVVKPNNQSWQSVIGEGFLEAGILPDNGFSLDHEAGTRLTGSTFD 218

Query: 181 ENGTRHGAVELLNKAQPTNLKVAVQAIVQRILF----SGLSANGVLYSDSKGKLHTAIIR 240
            NGTRH A ELLNK  P NL VAVQA V++ILF    S LSA GV+Y+DS G  H A +R
Sbjct: 219 NNGTRHAADELLNKGDPNNLLVAVQASVEKILFSSNTSNLSAIGVIYTDSDGNSHQAFVR 278

Query: 241 KKGEIIVSAGAIGSPQLLLLSGIGPKSYLSSLKLPVVLHQPHVGQSMVDYPRFGYVLAWP 300
             GE+IVSAG IG+PQLLLLSG+GP+SYLSSL + VV   P+VGQ + + PR       P
Sbjct: 279 GNGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNITVVQPNPYVGQFLYNNPRNFINNFPP 338

Query: 301 FPLAFTSSKVIGISQNKTFYFQSIASTTPLSIPPLFSIFPPNSTSLTTTSLATIGGKFSK 360
            P+  +   V+GI   ++ Y+Q   S+ P S PP FS+FP  S  L  ++ A I  +   
Sbjct: 339 NPIEASVVTVLGI---RSDYYQVSLSSLPFSTPP-FSLFPTTSYPLPNSTFAHIVSQVPG 398

Query: 361 VASTGSLRLNSSTDAASNPIVRFNYYSHPADVAMCVKGVRKVGDFLKTQTVENIKTRDLE 420
             S GS+ LNSS+D    P ++FNYYS+  D+A CV G++K+GD L+T+ +E  K RD+ 
Sbjct: 399 PLSHGSVTLNSSSDVRIAPNIKFNYYSNSTDLANCVSGMKKLGDLLRTKALEPYKARDVL 458

Query: 421 GNKTIQFVGLPLPGNLSDDSAVGEFCKKTVTSYWHYHGGCLVGKVVNGNYSVIGVKNLRV 480
           G     ++G+PLP N +DD++   FC   V SYWHYHGG LVGKV++ ++ V+G+K LRV
Sbjct: 459 GIDGFNYLGVPLPENQTDDASFETFCLDNVASYWHYHGGSLVGKVLDDSFRVMGIKALRV 518

Query: 481 LDGSTFAVSPGSNPTATLMMLAR 500
           +D STF   P S+P    +ML R
Sbjct: 519 VDASTFPYEPNSHPQGFYLMLGR 535

BLAST of Chy5G099740 vs. ExPASy Swiss-Prot
Match: O82784 ((R)-mandelonitrile lyase 4 OS=Prunus serotina OX=23207 GN=MDL4 PE=2 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 2.9e-133
Identity = 244/503 (48.51%), Postives = 336/503 (66.80%), Query Frame = 0

Query: 1   MKFVQDASDFPIKEEYDYIIVGGGTTGCPLAATLSKKFSVLLIERGSEPSKYPSVLNEQQ 60
           +KFV +A D  ++  YDYIIVGGGT+GCPLAATLS  +SVL++ERG+  ++YP+ L    
Sbjct: 40  LKFVYNAVDLELEGSYDYIIVGGGTSGCPLAATLSANYSVLVLERGTIATEYPNTLTVDG 99

Query: 61  LLNVFTVEDDGKNPFNRFISEDGVENIRGRVLGGSSMINGGVYSRADPEFFRTQLGMQEL 120
                  +DDGK P  RF+SEDG++N+R R+LGG+++IN GVY+RA+  F+    G+ E 
Sbjct: 100 FAYNLQQQDDGKTPVERFVSEDGIDNVRSRILGGTTIINAGVYARANESFYNNS-GV-EW 159

Query: 121 DMEMVEKAYEWVEEAIVFKPSLNLWQGAFRRFLVEGGVEPDNGFDLRDDVGTKISGSIFD 180
           D+++V +AYEWVE+AIV+KPS   WQ       +E GV PDNGF L  + GT+++GS FD
Sbjct: 160 DLDLVNEAYEWVEDAIVYKPSNQSWQSITGTAFLEAGVHPDNGFGLVHEEGTRLTGSTFD 219

Query: 181 ENGTRHGAVELLNKAQPTNLKVAVQAIVQRILF----SGLSANGVLYSDSKGKLHTAIIR 240
            +GTRH + ELLNK  P NLKVAV+A VQ+I+F    SGL+A GV+Y+DS G  H A++ 
Sbjct: 220 NSGTRHASDELLNKGDPDNLKVAVEAAVQKIIFSTESSGLTAVGVVYTDSNGTSHRALVS 279

Query: 241 KKGEIIVSAGAIGSPQLLLLSGIGPKSYLSSLKLPVVLHQPHVGQSMVDYPRFGYVLAWP 300
            KGE+I+SAG +G+PQLLLLSG+GP+SYL+SL + VV   P+VGQ + D PR    +  P
Sbjct: 280 GKGEVILSAGTLGTPQLLLLSGVGPESYLTSLNISVVASHPYVGQYVNDNPRNFINILPP 339

Query: 301 FPLAFTSSKVIGISQNKTFYFQSIASTTPLSIPPLFSIFPPNSTSLTTTSLATIGGKFSK 360
            P+  ++  V+GI+ +   ++Q   S+ P   PP FS+FP  S  L   + A I  K   
Sbjct: 340 NPIEPSTVTVLGITSD---FYQCSLSSLPFDTPP-FSLFPTTSYPLPNQTFAHIVSKVPG 399

Query: 361 VASTGSLRLNSSTDAASNPIVRFNYYSHPADVAMCVKGVRKVGDFLKTQTVENIKTRDLE 420
             S GSL L SS++ +  P V+FNY S P D+  CV G++K+G FL T  ++  K  DL 
Sbjct: 400 PLSAGSLTLQSSSNVSVAPNVKFNYCSDPVDLTHCVSGMKKIGVFLSTDALKPYKVDDLP 459

Query: 421 GNKTIQFVGLPLPGNLSDDSAVGEFCKKTVTSYWHYHGGCLVGKVVNGNYSVIGVKNLRV 480
           G      +G PLP N +DD+A  +FC+ TV SYWHYHGG +VGKV++GN+ V G+  LRV
Sbjct: 460 GIDGFNILGTPLPENQTDDAAFEKFCRDTVASYWHYHGGAIVGKVIDGNFRVTGINALRV 519

Query: 481 LDGSTFAVSPGSNPTATLMMLAR 500
           +DGSTF  +P S+P    +ML R
Sbjct: 520 VDGSTFPATPASHPQGFYLMLGR 536

BLAST of Chy5G099740 vs. ExPASy TrEMBL
Match: A0A0A0LJ76 (GMC_OxRdtase_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G049980 PE=3 SV=1)

HSP 1 Score: 975.7 bits (2521), Expect = 7.3e-281
Identity = 489/499 (98.00%), Postives = 495/499 (99.20%), Query Frame = 0

Query: 1   MKFVQDASDFPIKEEYDYIIVGGGTTGCPLAATLSKKFSVLLIERGSEPSKYPSVLNEQQ 60
           MKFVQDAS+FPIKEEYDYIIVGGGTTGCPLAATLSKKFSVLLIERGSEPSKYPSVLNEQQ
Sbjct: 1   MKFVQDASEFPIKEEYDYIIVGGGTTGCPLAATLSKKFSVLLIERGSEPSKYPSVLNEQQ 60

Query: 61  LLNVFTVEDDGKNPFNRFISEDGVENIRGRVLGGSSMINGGVYSRADPEFFRTQLGMQEL 120
           LLNVFTVEDDGKNPFNRFISEDGVENIRGRVLGGSSMINGGVYSRADPEFFRTQLGMQEL
Sbjct: 61  LLNVFTVEDDGKNPFNRFISEDGVENIRGRVLGGSSMINGGVYSRADPEFFRTQLGMQEL 120

Query: 121 DMEMVEKAYEWVEEAIVFKPSLNLWQGAFRRFLVEGGVEPDNGFDLRDDVGTKISGSIFD 180
           DMEMVEKAYEWVEEAIVFKPSLNLWQGAFRRFLVEGGVEPDNGFDLRDDVGTKISGSIFD
Sbjct: 121 DMEMVEKAYEWVEEAIVFKPSLNLWQGAFRRFLVEGGVEPDNGFDLRDDVGTKISGSIFD 180

Query: 181 ENGTRHGAVELLNKAQPTNLKVAVQAIVQRILFSGLSANGVLYSDSKGKLHTAIIRKKGE 240
           ENGTRHGAVELLNKAQPTNLKVAVQAIVQRILFSGLSANGVLYSDSKGKLHTA IRK+GE
Sbjct: 181 ENGTRHGAVELLNKAQPTNLKVAVQAIVQRILFSGLSANGVLYSDSKGKLHTAYIRKEGE 240

Query: 241 IIVSAGAIGSPQLLLLSGIGPKSYLSSLKLPVVLHQPHVGQSMVDYPRFGYVLAWPFPLA 300
           IIVSAGAIGSPQLLLLSGIGPKSYLSSL+LPVVLHQPHVGQSM+DYPRFGYVLAWPFPL+
Sbjct: 241 IIVSAGAIGSPQLLLLSGIGPKSYLSSLQLPVVLHQPHVGQSMMDYPRFGYVLAWPFPLS 300

Query: 301 FTSSKVIGISQNKTFYFQSIASTTPLSIPPLFSIFPPNSTSLTTTSLATIGGKFSKVAST 360
           FTSSKVIGISQNKTFYFQSIASTTPLSIPPLFSIFPPNSTSLTTTSLATIGGKFSKVAST
Sbjct: 301 FTSSKVIGISQNKTFYFQSIASTTPLSIPPLFSIFPPNSTSLTTTSLATIGGKFSKVAST 360

Query: 361 GSLRLNSSTDAASNPIVRFNYYSHPADVAMCVKGVRKVGDFLKTQTVENIKTRDLEGNKT 420
           GSLRLNSS DAASNPIVRFNYYSHPADVAMCVKGVRKVG FLKTQTVENIKTRDLEGNKT
Sbjct: 361 GSLRLNSSADAASNPIVRFNYYSHPADVAMCVKGVRKVGGFLKTQTVENIKTRDLEGNKT 420

Query: 421 IQFVGLPLPGNLSDDSAVGEFCKKTVTSYWHYHGGCLVGKVVNGNYSVIGVKNLRVLDGS 480
           IQFVGLPLPGNLSDDSAVGEFCKKTVT YWHYHGGCLVGKVV+GNYSVIGVKNLRVLDGS
Sbjct: 421 IQFVGLPLPGNLSDDSAVGEFCKKTVTLYWHYHGGCLVGKVVDGNYSVIGVKNLRVLDGS 480

Query: 481 TFAVSPGSNPTATLMMLAR 500
           TFAVSPGSNPTATLMMLAR
Sbjct: 481 TFAVSPGSNPTATLMMLAR 499

BLAST of Chy5G099740 vs. ExPASy TrEMBL
Match: A0A6J1K9H9 ((R)-mandelonitrile lyase 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492330 PE=3 SV=1)

HSP 1 Score: 797.3 bits (2058), Expect = 3.5e-227
Identity = 388/499 (77.76%), Postives = 442/499 (88.58%), Query Frame = 0

Query: 1   MKFVQDASDFPIKEEYDYIIVGGGTTGCPLAATLSKKFSVLLIERGSEPSKYPSVLNEQQ 60
           MKFV+D +DFP KEEYDYIIVGGGT GCPLAATLSK FS LLIERGSEP+KYPSVL EQ 
Sbjct: 39  MKFVRDGNDFPEKEEYDYIIVGGGTAGCPLAATLSKNFSTLLIERGSEPNKYPSVLKEQA 98

Query: 61  LLNVFTVEDDGKNPFNRFISEDGVENIRGRVLGGSSMINGGVYSRADPEFFRTQLGMQEL 120
           LLNVFTVEDDGKNPFNRF+S++GV+N+RGRVLGG+SMINGGVYS+ + +FF+TQLG+Q+ 
Sbjct: 99  LLNVFTVEDDGKNPFNRFVSKEGVQNLRGRVLGGTSMINGGVYSKPNTDFFKTQLGVQKF 158

Query: 121 DMEMVEKAYEWVEEAIVFKPSLNLWQGAFRRFLVEGGVEPDNGFDLRDDVGTKISGSIFD 180
           DMEMVEKAY+WVEEA+V++P LN WQ AFRR LVEGG+ PDNGF+LRD VGTKI+GSIFD
Sbjct: 159 DMEMVEKAYKWVEEALVYRPRLNAWQNAFRRVLVEGGIVPDNGFELRDVVGTKIAGSIFD 218

Query: 181 ENGTRHGAVELLNKAQPTNLKVAVQAIVQRILFSGLSANGVLYSDSKGKLHTAIIRKKGE 240
           ENG RHGA ELLNKA+P NLKVA++A V+RI+FSG SA GVLYSDSKGKLHTA+IRK GE
Sbjct: 219 ENGNRHGAEELLNKARPGNLKVAIRATVERIIFSGSSATGVLYSDSKGKLHTAVIRKNGE 278

Query: 241 IIVSAGAIGSPQLLLLSGIGPKSYLSSLKLPVVLHQPHVGQSMVDYPRFGYVLAWPFPLA 300
           IIVSAGAIGSPQLLLLSG+GPKS+LSSL LP++LH PHVGQ M D PR G +   P+PL 
Sbjct: 279 IIVSAGAIGSPQLLLLSGVGPKSHLSSLNLPLILHNPHVGQFMKDNPRVGVIPILPYPLD 338

Query: 301 FTSSKVIGISQNKTFYFQSIASTTPLSIPPLFSIFPPNSTSLTTTSLATIGGKFSKVAST 360
           FTSSKVIGIS N T+YFQSIA+T+P SIPP FS+ PPNSTS+  T +ATIGGKFSK+ S 
Sbjct: 339 FTSSKVIGISNNNTYYFQSIATTSPFSIPPSFSLLPPNSTSVNLT-IATIGGKFSKLTSV 398

Query: 361 GSLRLNSSTDAASNPIVRFNYYSHPADVAMCVKGVRKVGDFLKTQTVENIKTRDLEGNKT 420
           GSLRLNSSTD  + PIVRFNYYS+PAD+ +CV GVRKVGDFLKTQ ++NIKTRDLEGN+ 
Sbjct: 399 GSLRLNSSTDVKTTPIVRFNYYSNPADIQLCVGGVRKVGDFLKTQIIQNIKTRDLEGNRV 458

Query: 421 IQFVGLPLPGNLSDDSAVGEFCKKTVTSYWHYHGGCLVGKVVNGNYSVIGVKNLRVLDGS 480
           IQF+G PLP NLSD S+V EFCK+TVT+YWHYHGGCLVGKVV+GNYSVIG+KNLRVLDGS
Sbjct: 459 IQFLGPPLPENLSDYSSVAEFCKRTVTTYWHYHGGCLVGKVVDGNYSVIGLKNLRVLDGS 518

Query: 481 TFAVSPGSNPTATLMMLAR 500
           TFAVSPGSNP ATLMML R
Sbjct: 519 TFAVSPGSNPIATLMMLGR 536

BLAST of Chy5G099740 vs. ExPASy TrEMBL
Match: A0A6J1K7D5 ((R)-mandelonitrile lyase 1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111492330 PE=3 SV=1)

HSP 1 Score: 797.3 bits (2058), Expect = 3.5e-227
Identity = 388/499 (77.76%), Postives = 442/499 (88.58%), Query Frame = 0

Query: 1   MKFVQDASDFPIKEEYDYIIVGGGTTGCPLAATLSKKFSVLLIERGSEPSKYPSVLNEQQ 60
           MKFV+D +DFP KEEYDYIIVGGGT GCPLAATLSK FS LLIERGSEP+KYPSVL EQ 
Sbjct: 35  MKFVRDGNDFPEKEEYDYIIVGGGTAGCPLAATLSKNFSTLLIERGSEPNKYPSVLKEQA 94

Query: 61  LLNVFTVEDDGKNPFNRFISEDGVENIRGRVLGGSSMINGGVYSRADPEFFRTQLGMQEL 120
           LLNVFTVEDDGKNPFNRF+S++GV+N+RGRVLGG+SMINGGVYS+ + +FF+TQLG+Q+ 
Sbjct: 95  LLNVFTVEDDGKNPFNRFVSKEGVQNLRGRVLGGTSMINGGVYSKPNTDFFKTQLGVQKF 154

Query: 121 DMEMVEKAYEWVEEAIVFKPSLNLWQGAFRRFLVEGGVEPDNGFDLRDDVGTKISGSIFD 180
           DMEMVEKAY+WVEEA+V++P LN WQ AFRR LVEGG+ PDNGF+LRD VGTKI+GSIFD
Sbjct: 155 DMEMVEKAYKWVEEALVYRPRLNAWQNAFRRVLVEGGIVPDNGFELRDVVGTKIAGSIFD 214

Query: 181 ENGTRHGAVELLNKAQPTNLKVAVQAIVQRILFSGLSANGVLYSDSKGKLHTAIIRKKGE 240
           ENG RHGA ELLNKA+P NLKVA++A V+RI+FSG SA GVLYSDSKGKLHTA+IRK GE
Sbjct: 215 ENGNRHGAEELLNKARPGNLKVAIRATVERIIFSGSSATGVLYSDSKGKLHTAVIRKNGE 274

Query: 241 IIVSAGAIGSPQLLLLSGIGPKSYLSSLKLPVVLHQPHVGQSMVDYPRFGYVLAWPFPLA 300
           IIVSAGAIGSPQLLLLSG+GPKS+LSSL LP++LH PHVGQ M D PR G +   P+PL 
Sbjct: 275 IIVSAGAIGSPQLLLLSGVGPKSHLSSLNLPLILHNPHVGQFMKDNPRVGVIPILPYPLD 334

Query: 301 FTSSKVIGISQNKTFYFQSIASTTPLSIPPLFSIFPPNSTSLTTTSLATIGGKFSKVAST 360
           FTSSKVIGIS N T+YFQSIA+T+P SIPP FS+ PPNSTS+  T +ATIGGKFSK+ S 
Sbjct: 335 FTSSKVIGISNNNTYYFQSIATTSPFSIPPSFSLLPPNSTSVNLT-IATIGGKFSKLTSV 394

Query: 361 GSLRLNSSTDAASNPIVRFNYYSHPADVAMCVKGVRKVGDFLKTQTVENIKTRDLEGNKT 420
           GSLRLNSSTD  + PIVRFNYYS+PAD+ +CV GVRKVGDFLKTQ ++NIKTRDLEGN+ 
Sbjct: 395 GSLRLNSSTDVKTTPIVRFNYYSNPADIQLCVGGVRKVGDFLKTQIIQNIKTRDLEGNRV 454

Query: 421 IQFVGLPLPGNLSDDSAVGEFCKKTVTSYWHYHGGCLVGKVVNGNYSVIGVKNLRVLDGS 480
           IQF+G PLP NLSD S+V EFCK+TVT+YWHYHGGCLVGKVV+GNYSVIG+KNLRVLDGS
Sbjct: 455 IQFLGPPLPENLSDYSSVAEFCKRTVTTYWHYHGGCLVGKVVDGNYSVIGLKNLRVLDGS 514

Query: 481 TFAVSPGSNPTATLMMLAR 500
           TFAVSPGSNP ATLMML R
Sbjct: 515 TFAVSPGSNPIATLMMLGR 532

BLAST of Chy5G099740 vs. ExPASy TrEMBL
Match: A0A6J1EPL5 ((R)-mandelonitrile lyase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111436223 PE=3 SV=1)

HSP 1 Score: 797.0 bits (2057), Expect = 4.6e-227
Identity = 388/499 (77.76%), Postives = 443/499 (88.78%), Query Frame = 0

Query: 1   MKFVQDASDFPIKEEYDYIIVGGGTTGCPLAATLSKKFSVLLIERGSEPSKYPSVLNEQQ 60
           MKFV D +DFP KEEYDYIIVGGGT GCPLAATLSK FS LLIERGSEP+KYPSVL EQ 
Sbjct: 39  MKFVHDGNDFPEKEEYDYIIVGGGTAGCPLAATLSKNFSTLLIERGSEPNKYPSVLKEQA 98

Query: 61  LLNVFTVEDDGKNPFNRFISEDGVENIRGRVLGGSSMINGGVYSRADPEFFRTQLGMQEL 120
           LLNVFTVEDDGKNP+NRF S++GV+N+RGRVLGG+SMINGGVYS+ + +FF+TQLG+Q+ 
Sbjct: 99  LLNVFTVEDDGKNPYNRFTSKEGVQNLRGRVLGGTSMINGGVYSKPNTDFFKTQLGVQKF 158

Query: 121 DMEMVEKAYEWVEEAIVFKPSLNLWQGAFRRFLVEGGVEPDNGFDLRDDVGTKISGSIFD 180
           DMEMVEKAY+WVEEA+V++PSLN WQ AFRR LVEGG+ PDNGF+LRD VGTKI+GSIFD
Sbjct: 159 DMEMVEKAYKWVEEALVYRPSLNAWQNAFRRILVEGGIVPDNGFELRDVVGTKITGSIFD 218

Query: 181 ENGTRHGAVELLNKAQPTNLKVAVQAIVQRILFSGLSANGVLYSDSKGKLHTAIIRKKGE 240
           ENG RHGAVELLNKA+P NLKVA++A V+RI+FSG SA+ VLYSDSKGKLHTA++ K GE
Sbjct: 219 ENGNRHGAVELLNKARPGNLKVAIRATVERIIFSGSSASEVLYSDSKGKLHTAVVHKNGE 278

Query: 241 IIVSAGAIGSPQLLLLSGIGPKSYLSSLKLPVVLHQPHVGQSMVDYPRFGYVLAWPFPLA 300
           II+SAGAIGSPQLLLLSG+GPKS+LSSL +P++LH PHVG+ M D PR G V   PFPL 
Sbjct: 279 IILSAGAIGSPQLLLLSGVGPKSHLSSLNIPLILHNPHVGEFMKDNPRVGVVPILPFPLD 338

Query: 301 FTSSKVIGISQNKTFYFQSIASTTPLSIPPLFSIFPPNSTSLTTTSLATIGGKFSKVAST 360
           FTSSKVIGIS N T+YFQSIAST+P SIPP FS+ PPNSTS+  T +ATIGGKFSK+AS 
Sbjct: 339 FTSSKVIGISNNNTYYFQSIASTSPFSIPPSFSLLPPNSTSVNLT-VATIGGKFSKLASV 398

Query: 361 GSLRLNSSTDAASNPIVRFNYYSHPADVAMCVKGVRKVGDFLKTQTVENIKTRDLEGNKT 420
           GSLRLNSSTD  +NPIVRFNYYS+PAD+ +CV GVRKVGDFLKTQ ++NIKTRDLEGN+ 
Sbjct: 399 GSLRLNSSTDVKTNPIVRFNYYSNPADIQLCVGGVRKVGDFLKTQIIQNIKTRDLEGNRV 458

Query: 421 IQFVGLPLPGNLSDDSAVGEFCKKTVTSYWHYHGGCLVGKVVNGNYSVIGVKNLRVLDGS 480
           IQF+G PLP NLSD S+V EFCK+TVT+YWHYHGGCLVGKVV+GNYSVIG+KNLRVLDGS
Sbjct: 459 IQFLGPPLPENLSDYSSVAEFCKRTVTTYWHYHGGCLVGKVVDGNYSVIGLKNLRVLDGS 518

Query: 481 TFAVSPGSNPTATLMMLAR 500
           TFAVSPGSNP ATLMML R
Sbjct: 519 TFAVSPGSNPIATLMMLGR 536

BLAST of Chy5G099740 vs. ExPASy TrEMBL
Match: A0A0A0LK76 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G055000 PE=3 SV=1)

HSP 1 Score: 709.9 bits (1831), Expect = 7.4e-201
Identity = 358/499 (71.74%), Postives = 411/499 (82.36%), Query Frame = 0

Query: 1   MKFVQDASDFPIKEEYDYIIVGGGTTGCPLAATLSKKFSVLLIERGSEPSKYPSVLNEQQ 60
           MKFV +A+D P KEEYDYII+GGGT GCPLA TLS KFSVLL+ERGS+P+KYPSVL+EQ 
Sbjct: 36  MKFVHNATDLPPKEEYDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLDEQG 95

Query: 61  LLNVFTVEDDGKNPFNRFISEDGVENIRGRVLGGSSMINGGVYSRADPEFFRTQLGMQEL 120
           LLNVF   DDG+NPF RF+SEDGVENIRGRVLGG SM+N G YSR   EFF +     + 
Sbjct: 96  LLNVFAAGDDGRNPFQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHREFFAS--AGVDW 155

Query: 121 DMEMVEKAYEWVEEAIVFKPSLNLWQGAFRRFLVEGGVEPDNGFDLRDDVGTKISGSIFD 180
           DME+VEKAYEWVEE +V +P LN WQ AFR  L+EGGV PD+GFDLR  VGTK  GSIFD
Sbjct: 156 DMELVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGVVPDSGFDLRHLVGTKTGGSIFD 215

Query: 181 ENGTRHGAVELLNKAQPTNLKVAVQAIVQRILFSGLSANGVLYSDSKGKLHTAIIRKKGE 240
             G RHGAVELLNKA PTN+KVA++A VQRILFSGLSANGVLYSDSKGKLH AIIRKKGE
Sbjct: 216 NKGNRHGAVELLNKANPTNIKVAIEATVQRILFSGLSANGVLYSDSKGKLHRAIIRKKGE 275

Query: 241 IIVSAGAIGSPQLLLLSGIGPKSYLSSLKLPVVLHQPHVGQSMVDYPRFGYVLAWPFPLA 300
           IIVSAGAIGSPQLLLLSGIGPKS+LSSLKLPVVLHQP+VGQSM D PRFG  +  PFP+ 
Sbjct: 276 IIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVGQSMSDNPRFGTNIIIPFPVL 335

Query: 301 FTSSKVIGISQNKTFYFQSIASTTPLSIPPLFSIFPPNSTSLTTTSLATIGGKFSKVAST 360
            +S KV+GI Q+   Y QSIAS  P+ IP +FS+ PP +TS+  T LA   GKFS+V S 
Sbjct: 336 PSSVKVVGILQD-NIYIQSIASPFPILIPQIFSLLPPQATSIIPT-LAMFVGKFSEVHSE 395

Query: 361 GSLRLNSSTDAASNPIVRFNYYSHPADVAMCVKGVRKVGDFLKTQTVENIKTRDLEGNKT 420
           GSLRLNSST+   +PIV FNYYSHP D+  CVKGVRK+GD LKT+T+E IKT++LEGNK 
Sbjct: 396 GSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVKGVRKMGDLLKTRTMEKIKTKNLEGNKG 455

Query: 421 IQFVGLPLPGNLSDDSAVGEFCKKTVTSYWHYHGGCLVGKVVNGNYSVIGVKNLRVLDGS 480
            +F+G+PLP NL +DS+V E+CKKTVT+YWHYHGGCLVGKVV+GNY VIG+KNLRV+DGS
Sbjct: 456 FEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGCLVGKVVDGNYKVIGIKNLRVVDGS 515

Query: 481 TFAVSPGSNPTATLMMLAR 500
           TF+ SPG+NP ATLMML R
Sbjct: 516 TFSESPGTNPMATLMMLGR 530

BLAST of Chy5G099740 vs. NCBI nr
Match: XP_011648921.2 ((R)-mandelonitrile lyase 1 [Cucumis sativus])

HSP 1 Score: 974 bits (2518), Expect = 0.0
Identity = 489/499 (98.00%), Postives = 495/499 (99.20%), Query Frame = 0

Query: 1   MKFVQDASDFPIKEEYDYIIVGGGTTGCPLAATLSKKFSVLLIERGSEPSKYPSVLNEQQ 60
           MKFVQDAS+FPIKEEYDYIIVGGGTTGCPLAATLSKKFSVLLIERGSEPSKYPSVLNEQQ
Sbjct: 32  MKFVQDASEFPIKEEYDYIIVGGGTTGCPLAATLSKKFSVLLIERGSEPSKYPSVLNEQQ 91

Query: 61  LLNVFTVEDDGKNPFNRFISEDGVENIRGRVLGGSSMINGGVYSRADPEFFRTQLGMQEL 120
           LLNVFTVEDDGKNPFNRFISEDGVENIRGRVLGGSSMINGGVYSRADPEFFRTQLGMQEL
Sbjct: 92  LLNVFTVEDDGKNPFNRFISEDGVENIRGRVLGGSSMINGGVYSRADPEFFRTQLGMQEL 151

Query: 121 DMEMVEKAYEWVEEAIVFKPSLNLWQGAFRRFLVEGGVEPDNGFDLRDDVGTKISGSIFD 180
           DMEMVEKAYEWVEEAIVFKPSLNLWQGAFRRFLVEGGVEPDNGFDLRDDVGTKISGSIFD
Sbjct: 152 DMEMVEKAYEWVEEAIVFKPSLNLWQGAFRRFLVEGGVEPDNGFDLRDDVGTKISGSIFD 211

Query: 181 ENGTRHGAVELLNKAQPTNLKVAVQAIVQRILFSGLSANGVLYSDSKGKLHTAIIRKKGE 240
           ENGTRHGAVELLNKAQPTNLKVAVQAIVQRILFSGLSANGVLYSDSKGKLHTA IRK+GE
Sbjct: 212 ENGTRHGAVELLNKAQPTNLKVAVQAIVQRILFSGLSANGVLYSDSKGKLHTAYIRKEGE 271

Query: 241 IIVSAGAIGSPQLLLLSGIGPKSYLSSLKLPVVLHQPHVGQSMVDYPRFGYVLAWPFPLA 300
           IIVSAGAIGSPQLLLLSGIGPKSYLSSL+LPVVLHQPHVGQSM+DYPRFGYVLAWPFPL+
Sbjct: 272 IIVSAGAIGSPQLLLLSGIGPKSYLSSLQLPVVLHQPHVGQSMMDYPRFGYVLAWPFPLS 331

Query: 301 FTSSKVIGISQNKTFYFQSIASTTPLSIPPLFSIFPPNSTSLTTTSLATIGGKFSKVAST 360
           FTSSKVIGISQNKTFYFQSIASTTPLSIPPLFSIFPPNSTSLTTTSLATIGGKFSKVAST
Sbjct: 332 FTSSKVIGISQNKTFYFQSIASTTPLSIPPLFSIFPPNSTSLTTTSLATIGGKFSKVAST 391

Query: 361 GSLRLNSSTDAASNPIVRFNYYSHPADVAMCVKGVRKVGDFLKTQTVENIKTRDLEGNKT 420
           GSLRLNSS DAASNPIVRFNYYSHPADVAMCVKGVRKVG FLKTQTVENIKTRDLEGNKT
Sbjct: 392 GSLRLNSSADAASNPIVRFNYYSHPADVAMCVKGVRKVGGFLKTQTVENIKTRDLEGNKT 451

Query: 421 IQFVGLPLPGNLSDDSAVGEFCKKTVTSYWHYHGGCLVGKVVNGNYSVIGVKNLRVLDGS 480
           IQFVGLPLPGNLSDDSAVGEFCKKTVT YWHYHGGCLVGKVV+GNYSVIGVKNLRVLDGS
Sbjct: 452 IQFVGLPLPGNLSDDSAVGEFCKKTVTLYWHYHGGCLVGKVVDGNYSVIGVKNLRVLDGS 511

Query: 481 TFAVSPGSNPTATLMMLAR 499
           TFAVSPGSNPTATLMMLAR
Sbjct: 512 TFAVSPGSNPTATLMMLAR 530

BLAST of Chy5G099740 vs. NCBI nr
Match: XP_038889318.1 ((R)-mandelonitrile lyase 3-like [Benincasa hispida])

HSP 1 Score: 885 bits (2286), Expect = 0.0
Identity = 440/499 (88.18%), Postives = 471/499 (94.39%), Query Frame = 0

Query: 1   MKFVQDASDFPIKEEYDYIIVGGGTTGCPLAATLSKKFSVLLIERGSEPSKYPSVLNEQQ 60
           MKFV++AS+FP KEEYDYIIVGGGTTGCPLAATLSK FS LLIERGSEPSKYPSVLNEQ+
Sbjct: 65  MKFVRNASEFPEKEEYDYIIVGGGTTGCPLAATLSKNFSTLLIERGSEPSKYPSVLNEQE 124

Query: 61  LLNVFTVEDDGKNPFNRFISEDGVENIRGRVLGGSSMINGGVYSRADPEFFRTQLGMQEL 120
           LLNVFTVEDDGKNPFNRFISEDGVENIRGR+LGG+SMINGGVYSRA+PEFF+TQLGMQEL
Sbjct: 125 LLNVFTVEDDGKNPFNRFISEDGVENIRGRILGGTSMINGGVYSRANPEFFKTQLGMQEL 184

Query: 121 DMEMVEKAYEWVEEAIVFKPSLNLWQGAFRRFLVEGGVEPDNGFDLRDDVGTKISGSIFD 180
           DMEMVEKAY+WVEEAIV+KPSLNLWQ AFRRFLVEGGV+PDNGFDLRD VGTKISGSIFD
Sbjct: 185 DMEMVEKAYQWVEEAIVYKPSLNLWQAAFRRFLVEGGVQPDNGFDLRDIVGTKISGSIFD 244

Query: 181 ENGTRHGAVELLNKAQPTNLKVAVQAIVQRILFSGLSANGVLYSDSKGKLHTAIIRKKGE 240
           ENG RHGAVELLNKA+PTNLKVAVQA VQRILFSGLSA GVLYSDSKGKLHTA IR+KGE
Sbjct: 245 ENGIRHGAVELLNKAKPTNLKVAVQATVQRILFSGLSARGVLYSDSKGKLHTAFIREKGE 304

Query: 241 IIVSAGAIGSPQLLLLSGIGPKSYLSSLKLPVVLHQPHVGQSMVDYPRFGYVLAWPFPLA 300
           II+SAGA+GSPQLLLLSG+GPKSYLSSLKL VVL+QPHVGQ M D PRFG +LA  F LA
Sbjct: 305 IILSAGALGSPQLLLLSGVGPKSYLSSLKLRVVLNQPHVGQFMSDNPRFGIILALSFRLA 364

Query: 301 FTSSKVIGISQNKTFYFQSIASTTPLSIPPLFSIFPPNSTSLTTTSLATIGGKFSKVAST 360
           F+SSKVIGISQN ++YFQSIASTTPLSIPPLFSIFPPNSTSLTTTSLATIGGKFSK++S 
Sbjct: 365 FSSSKVIGISQNNSYYFQSIASTTPLSIPPLFSIFPPNSTSLTTTSLATIGGKFSKISSF 424

Query: 361 GSLRLNSSTDAASNPIVRFNYYSHPADVAMCVKGVRKVGDFLKTQTVENIKTRDLEGNKT 420
           GSL LNSSTD  +NPIVRFNYYSHP D+A CV+GVRKVGDFLKT T+ENIKTRDLEGNKT
Sbjct: 425 GSLHLNSSTDVKNNPIVRFNYYSHPDDLAKCVRGVRKVGDFLKTPTIENIKTRDLEGNKT 484

Query: 421 IQFVGLPLPGNLSDDSAVGEFCKKTVTSYWHYHGGCLVGKVVNGNYSVIGVKNLRVLDGS 480
           +QFVGLPLP NLSDD+ VGEFCKKTVTSYWHYHGGCLVGKVV+ NYSVIG+KNLRVLDGS
Sbjct: 485 LQFVGLPLPENLSDDTLVGEFCKKTVTSYWHYHGGCLVGKVVDSNYSVIGIKNLRVLDGS 544

Query: 481 TFAVSPGSNPTATLMMLAR 499
           TFAVSPGSNPTATLMMLAR
Sbjct: 545 TFAVSPGSNPTATLMMLAR 563

BLAST of Chy5G099740 vs. NCBI nr
Match: XP_023546914.1 ((R)-mandelonitrile lyase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 797 bits (2059), Expect = 6.53e-287
Identity = 389/499 (77.96%), Postives = 444/499 (88.98%), Query Frame = 0

Query: 1   MKFVQDASDFPIKEEYDYIIVGGGTTGCPLAATLSKKFSVLLIERGSEPSKYPSVLNEQQ 60
           MKFV+D +DFP KEEYDYIIVGGGT GCPLAATLSK FS LLIERGSEP+KYPSVL EQ 
Sbjct: 39  MKFVRDGNDFPEKEEYDYIIVGGGTAGCPLAATLSKNFSTLLIERGSEPNKYPSVLKEQA 98

Query: 61  LLNVFTVEDDGKNPFNRFISEDGVENIRGRVLGGSSMINGGVYSRADPEFFRTQLGMQEL 120
           LLNVFTVEDDGKNP+NRF S++GV+N+RGRVLGG+SMINGGVYS+ + +FF+TQLG+Q+ 
Sbjct: 99  LLNVFTVEDDGKNPYNRFTSKEGVQNLRGRVLGGTSMINGGVYSKPNTDFFKTQLGVQKF 158

Query: 121 DMEMVEKAYEWVEEAIVFKPSLNLWQGAFRRFLVEGGVEPDNGFDLRDDVGTKISGSIFD 180
           DMEMVEKAY+WVEEA+V++PSLN WQ AFRR LVEGG+ PDNGF+LRD VGTKI+GSIFD
Sbjct: 159 DMEMVEKAYKWVEEALVYRPSLNAWQNAFRRILVEGGIVPDNGFELRDVVGTKITGSIFD 218

Query: 181 ENGTRHGAVELLNKAQPTNLKVAVQAIVQRILFSGLSANGVLYSDSKGKLHTAIIRKKGE 240
           +NG RHGAVELLNKA+P NLKVA++A V+RI+FSG SANGVLYSDSKGK HTA+IRK GE
Sbjct: 219 DNGNRHGAVELLNKARPRNLKVAIRATVERIIFSGSSANGVLYSDSKGKQHTAVIRKNGE 278

Query: 241 IIVSAGAIGSPQLLLLSGIGPKSYLSSLKLPVVLHQPHVGQSMVDYPRFGYVLAWPFPLA 300
           IIVSAGAIGSPQLLLLSG+GPKS+LSSL LP++LH PHVG+ M D PR G V   PFPL 
Sbjct: 279 IIVSAGAIGSPQLLLLSGVGPKSHLSSLNLPLILHNPHVGEFMKDNPRVGVVPILPFPLD 338

Query: 301 FTSSKVIGISQNKTFYFQSIASTTPLSIPPLFSIFPPNSTSLTTTSLATIGGKFSKVAST 360
           FTSSKVIGIS N T+YFQSIAST+P SIPP FS+ PPNST++   S+ATIGGKFSK+ S 
Sbjct: 339 FTSSKVIGISTNNTYYFQSIASTSPFSIPPSFSLLPPNSTTVNL-SIATIGGKFSKLTSV 398

Query: 361 GSLRLNSSTDAASNPIVRFNYYSHPADVAMCVKGVRKVGDFLKTQTVENIKTRDLEGNKT 420
           GSLRLNSSTD  ++PIVRFNYYS+PAD+ +CV GVRKVGDFLKTQ ++NIKTRDLEGN+ 
Sbjct: 399 GSLRLNSSTDVKTSPIVRFNYYSNPADIQLCVGGVRKVGDFLKTQIIQNIKTRDLEGNRV 458

Query: 421 IQFVGLPLPGNLSDDSAVGEFCKKTVTSYWHYHGGCLVGKVVNGNYSVIGVKNLRVLDGS 480
           IQF+G PLP NLSD S+V EFCK+TVT+YWHYHGGCLVGKVV+GNYSVIG+KNLRVLDGS
Sbjct: 459 IQFLGPPLPENLSDYSSVAEFCKRTVTTYWHYHGGCLVGKVVDGNYSVIGLKNLRVLDGS 518

Query: 481 TFAVSPGSNPTATLMMLAR 499
           TFAVSPGSNP ATLMML R
Sbjct: 519 TFAVSPGSNPIATLMMLGR 536

BLAST of Chy5G099740 vs. NCBI nr
Match: XP_022997401.1 ((R)-mandelonitrile lyase 1-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 796 bits (2055), Expect = 2.29e-286
Identity = 388/499 (77.76%), Postives = 442/499 (88.58%), Query Frame = 0

Query: 1   MKFVQDASDFPIKEEYDYIIVGGGTTGCPLAATLSKKFSVLLIERGSEPSKYPSVLNEQQ 60
           MKFV+D +DFP KEEYDYIIVGGGT GCPLAATLSK FS LLIERGSEP+KYPSVL EQ 
Sbjct: 35  MKFVRDGNDFPEKEEYDYIIVGGGTAGCPLAATLSKNFSTLLIERGSEPNKYPSVLKEQA 94

Query: 61  LLNVFTVEDDGKNPFNRFISEDGVENIRGRVLGGSSMINGGVYSRADPEFFRTQLGMQEL 120
           LLNVFTVEDDGKNPFNRF+S++GV+N+RGRVLGG+SMINGGVYS+ + +FF+TQLG+Q+ 
Sbjct: 95  LLNVFTVEDDGKNPFNRFVSKEGVQNLRGRVLGGTSMINGGVYSKPNTDFFKTQLGVQKF 154

Query: 121 DMEMVEKAYEWVEEAIVFKPSLNLWQGAFRRFLVEGGVEPDNGFDLRDDVGTKISGSIFD 180
           DMEMVEKAY+WVEEA+V++P LN WQ AFRR LVEGG+ PDNGF+LRD VGTKI+GSIFD
Sbjct: 155 DMEMVEKAYKWVEEALVYRPRLNAWQNAFRRVLVEGGIVPDNGFELRDVVGTKIAGSIFD 214

Query: 181 ENGTRHGAVELLNKAQPTNLKVAVQAIVQRILFSGLSANGVLYSDSKGKLHTAIIRKKGE 240
           ENG RHGA ELLNKA+P NLKVA++A V+RI+FSG SA GVLYSDSKGKLHTA+IRK GE
Sbjct: 215 ENGNRHGAEELLNKARPGNLKVAIRATVERIIFSGSSATGVLYSDSKGKLHTAVIRKNGE 274

Query: 241 IIVSAGAIGSPQLLLLSGIGPKSYLSSLKLPVVLHQPHVGQSMVDYPRFGYVLAWPFPLA 300
           IIVSAGAIGSPQLLLLSG+GPKS+LSSL LP++LH PHVGQ M D PR G +   P+PL 
Sbjct: 275 IIVSAGAIGSPQLLLLSGVGPKSHLSSLNLPLILHNPHVGQFMKDNPRVGVIPILPYPLD 334

Query: 301 FTSSKVIGISQNKTFYFQSIASTTPLSIPPLFSIFPPNSTSLTTTSLATIGGKFSKVAST 360
           FTSSKVIGIS N T+YFQSIA+T+P SIPP FS+ PPNSTS+  T +ATIGGKFSK+ S 
Sbjct: 335 FTSSKVIGISNNNTYYFQSIATTSPFSIPPSFSLLPPNSTSVNLT-IATIGGKFSKLTSV 394

Query: 361 GSLRLNSSTDAASNPIVRFNYYSHPADVAMCVKGVRKVGDFLKTQTVENIKTRDLEGNKT 420
           GSLRLNSSTD  + PIVRFNYYS+PAD+ +CV GVRKVGDFLKTQ ++NIKTRDLEGN+ 
Sbjct: 395 GSLRLNSSTDVKTTPIVRFNYYSNPADIQLCVGGVRKVGDFLKTQIIQNIKTRDLEGNRV 454

Query: 421 IQFVGLPLPGNLSDDSAVGEFCKKTVTSYWHYHGGCLVGKVVNGNYSVIGVKNLRVLDGS 480
           IQF+G PLP NLSD S+V EFCK+TVT+YWHYHGGCLVGKVV+GNYSVIG+KNLRVLDGS
Sbjct: 455 IQFLGPPLPENLSDYSSVAEFCKRTVTTYWHYHGGCLVGKVVDGNYSVIGLKNLRVLDGS 514

Query: 481 TFAVSPGSNPTATLMMLAR 499
           TFAVSPGSNP ATLMML R
Sbjct: 515 TFAVSPGSNPIATLMMLGR 532

BLAST of Chy5G099740 vs. NCBI nr
Match: XP_022997400.1 ((R)-mandelonitrile lyase 1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 796 bits (2055), Expect = 2.65e-286
Identity = 388/499 (77.76%), Postives = 442/499 (88.58%), Query Frame = 0

Query: 1   MKFVQDASDFPIKEEYDYIIVGGGTTGCPLAATLSKKFSVLLIERGSEPSKYPSVLNEQQ 60
           MKFV+D +DFP KEEYDYIIVGGGT GCPLAATLSK FS LLIERGSEP+KYPSVL EQ 
Sbjct: 39  MKFVRDGNDFPEKEEYDYIIVGGGTAGCPLAATLSKNFSTLLIERGSEPNKYPSVLKEQA 98

Query: 61  LLNVFTVEDDGKNPFNRFISEDGVENIRGRVLGGSSMINGGVYSRADPEFFRTQLGMQEL 120
           LLNVFTVEDDGKNPFNRF+S++GV+N+RGRVLGG+SMINGGVYS+ + +FF+TQLG+Q+ 
Sbjct: 99  LLNVFTVEDDGKNPFNRFVSKEGVQNLRGRVLGGTSMINGGVYSKPNTDFFKTQLGVQKF 158

Query: 121 DMEMVEKAYEWVEEAIVFKPSLNLWQGAFRRFLVEGGVEPDNGFDLRDDVGTKISGSIFD 180
           DMEMVEKAY+WVEEA+V++P LN WQ AFRR LVEGG+ PDNGF+LRD VGTKI+GSIFD
Sbjct: 159 DMEMVEKAYKWVEEALVYRPRLNAWQNAFRRVLVEGGIVPDNGFELRDVVGTKIAGSIFD 218

Query: 181 ENGTRHGAVELLNKAQPTNLKVAVQAIVQRILFSGLSANGVLYSDSKGKLHTAIIRKKGE 240
           ENG RHGA ELLNKA+P NLKVA++A V+RI+FSG SA GVLYSDSKGKLHTA+IRK GE
Sbjct: 219 ENGNRHGAEELLNKARPGNLKVAIRATVERIIFSGSSATGVLYSDSKGKLHTAVIRKNGE 278

Query: 241 IIVSAGAIGSPQLLLLSGIGPKSYLSSLKLPVVLHQPHVGQSMVDYPRFGYVLAWPFPLA 300
           IIVSAGAIGSPQLLLLSG+GPKS+LSSL LP++LH PHVGQ M D PR G +   P+PL 
Sbjct: 279 IIVSAGAIGSPQLLLLSGVGPKSHLSSLNLPLILHNPHVGQFMKDNPRVGVIPILPYPLD 338

Query: 301 FTSSKVIGISQNKTFYFQSIASTTPLSIPPLFSIFPPNSTSLTTTSLATIGGKFSKVAST 360
           FTSSKVIGIS N T+YFQSIA+T+P SIPP FS+ PPNSTS+  T +ATIGGKFSK+ S 
Sbjct: 339 FTSSKVIGISNNNTYYFQSIATTSPFSIPPSFSLLPPNSTSVNLT-IATIGGKFSKLTSV 398

Query: 361 GSLRLNSSTDAASNPIVRFNYYSHPADVAMCVKGVRKVGDFLKTQTVENIKTRDLEGNKT 420
           GSLRLNSSTD  + PIVRFNYYS+PAD+ +CV GVRKVGDFLKTQ ++NIKTRDLEGN+ 
Sbjct: 399 GSLRLNSSTDVKTTPIVRFNYYSNPADIQLCVGGVRKVGDFLKTQIIQNIKTRDLEGNRV 458

Query: 421 IQFVGLPLPGNLSDDSAVGEFCKKTVTSYWHYHGGCLVGKVVNGNYSVIGVKNLRVLDGS 480
           IQF+G PLP NLSD S+V EFCK+TVT+YWHYHGGCLVGKVV+GNYSVIG+KNLRVLDGS
Sbjct: 459 IQFLGPPLPENLSDYSSVAEFCKRTVTTYWHYHGGCLVGKVVDGNYSVIGLKNLRVLDGS 518

Query: 481 TFAVSPGSNPTATLMMLAR 499
           TFAVSPGSNP ATLMML R
Sbjct: 519 TFAVSPGSNPIATLMMLGR 536

BLAST of Chy5G099740 vs. TAIR 10
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 474.2 bits (1219), Expect = 1.3e-133
Identity = 248/507 (48.92%), Postives = 337/507 (66.47%), Query Frame = 0

Query: 1   MKFVQDASDFPIKEEYDYIIVGGGTTGCPLAATLSKKFSVLLIERGSEPSKYPSVLNEQQ 60
           M+F+ +A+DF  ++ YDYIIVGGGT GCPLAATLS+ F VLL+ERG  P   P+V++   
Sbjct: 39  MRFISNATDFASEDYYDYIIVGGGTAGCPLAATLSQSFRVLLLERGGVPYNRPNVMSHDG 98

Query: 61  LLNVFTVEDDGKNPFNRFISEDGVENIRGRVLGGSSMINGGVYSRADPEFFRTQLGMQEL 120
            L   T  ++  +P   FISE+GV N RGRVLGGSS IN G YSRAD +FF    G+   
Sbjct: 99  FLTTLTDVNNFDSPAQSFISEEGVPNARGRVLGGSSAINAGFYSRADKQFFENS-GL-VW 158

Query: 121 DMEMVEKAYEWVEEAIVFKPSLNLWQGAFRRFLVEGGVEPDNGFDLRDDVGTKISGSIFD 180
           D+  V ++YEWVE AIVF+P L  WQ A R  L+E GV P NGF L   VGTKI GS FD
Sbjct: 159 DLSSVNQSYEWVERAIVFRPQLRTWQTAIRDALLEVGVHPFNGFTLEHKVGTKIGGSTFD 218

Query: 181 ENGTRHGAVELLNKAQPTNLKVAVQAIVQRILF--------SGLSANGVLYSDSKGKLHT 240
             G RH + +LL  A+ +N++VAV A V+R+L         S +SA GV+Y D  G+ H 
Sbjct: 219 RTGRRHSSADLLRYARSSNIRVAVYATVERVLLASSPSVSGSNVSAIGVVYRDQLGRFHH 278

Query: 241 AIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSYLSSLKLPVVLHQPHVGQSMVDYPRFGYV 300
           A+IR +GE+I+SAGA+GSPQLL LSGIGP+SYLS+  +PV L QPHVG  + D PR G  
Sbjct: 279 ALIRDRGEVILSAGALGSPQLLFLSGIGPRSYLSTWGIPVALDQPHVGDFVYDNPRNGIS 338

Query: 301 LAWPFPLAFTSSKVIGISQNKTFYFQSIASTTPLSIPPLFSIFPPNSTSLTTTSLATIGG 360
           +  P P+  +  +V+G++++  F  ++ ++  P +  PL S+F     S     + TI  
Sbjct: 339 IVPPVPMENSLIQVVGVTEDGAF-LEAASNVIPFA-SPLHSVFIRAPASPLYVPVTTIME 398

Query: 361 KFSKVASTGSLRLNSSTDAASNPIVRFNYYSHPADVAMCVKGVRKVGDFLKTQTVENIKT 420
           K     S G LRL +STD   NP+VRFNY+S P D+  CV G RK+G+ L+++ +++   
Sbjct: 399 KILGPVSIGLLRL-ASTDVRINPVVRFNYFSDPQDLERCVNGTRKIGEILRSRAMQDFMI 458

Query: 421 RDLEGNKTIQFVGLPLPGNLSDDSAVGEFCKKTVTSYWHYHGGCLVGKVVNGNYSVIGVK 480
           R+  GN+  +FVG PLP + S+D  + +FC++TV++ WHYHGG +VGKVV+ +  VIGV 
Sbjct: 459 REWFGNRRFRFVGAPLPVDQSNDLVMADFCRRTVSTIWHYHGGAVVGKVVDSDLKVIGVN 518

Query: 481 NLRVLDGSTFAVSPGSNPTATLMMLAR 500
           +LR++DGSTF +SPG+NP ATLMML R
Sbjct: 519 SLRLVDGSTFNISPGTNPQATLMMLGR 540

BLAST of Chy5G099740 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 359.8 bits (922), Expect = 3.6e-99
Identity = 210/533 (39.40%), Postives = 293/533 (54.97%), Query Frame = 0

Query: 3   FVQDASDFPIKEEYDYIIVGGGTTGCPLAATLSKKFSVLLIERGSEPSKYPSVLNEQQLL 62
           F++DA+  P    YDYII+GGGT GCPLAATLS+  SVLL+ERG  P   P++       
Sbjct: 33  FMRDATGSPTTSYYDYIIIGGGTAGCPLAATLSQNASVLLLERGDSPYNNPNI-TRLSAF 92

Query: 63  NVFTVEDDGKNPFNRFISEDGVENIRGRVLGGSSMINGGVYSRADPEFFRTQLGMQELDM 122
                +    +P  RF+SEDGV N R RVLGG S +N G Y+RA  ++ R        D 
Sbjct: 93  GAALSDLSESSPSQRFVSEDGVINARARVLGGGSALNAGFYTRAGTKYVRN----MGWDG 152

Query: 123 EMVEKAYEWVEEAIVFKPSLNLWQGAFRRFLVEGGVEPDNGFDLRDDVGTKISGSIFDEN 182
            +  ++Y+WVE  + F+P +  WQ A R  L+E G+ P+NGF      GTK  G+IFD N
Sbjct: 153 ALANESYQWVEAKVAFQPPMGRWQTAVRDGLLEAGIVPNNGFTYDHINGTKFGGTIFDRN 212

Query: 183 GTRHGAVELLNKAQPTNLKVAVQAIVQRILFSGLS-----ANGVLYSDSKGKLHTAIIRK 242
           G RH A +LL  A P  + V + A V RILF         ANGV+Y D  G+ H A +++
Sbjct: 213 GNRHTAADLLEYADPKGITVLLHATVHRILFRTRGTTKPIANGVVYRDRTGQAHRAYLKE 272

Query: 243 --KGEIIVSAGAIGSPQLLLLSGIGPKSYLSSLKLPVVLHQPHVGQSMVDYPRFGYVLAW 302
               EII+SAG +GSPQLL+LSG+GP + L +  + VV+ QPHVGQ M D P     +  
Sbjct: 273 GALSEIILSAGTLGSPQLLMLSGVGPSAQLQAQNITVVMDQPHVGQGMYDNPMNAVFVPS 332

Query: 303 PFPLAFTSSKVIGISQNKTFYFQS---------IASTTPLSIPPLFSIFPPNSTSLTTTS 362
           P P+  +  +V+GI+   T+   +           S+   S    +++F P +T L + S
Sbjct: 333 PVPVEVSLIEVVGITGEGTYVEAAGGENFGGGGGGSSGSSSTRDYYAMFSPRATLLESNS 392

Query: 363 LATI-------GG----KFSKVASTGSLRLNSSTDAASNPIVRFNYYSHPADVAMCVKGV 422
           +  +       GG    K     STG L L  + +   NPIV FNY+ HP D+  CV+G+
Sbjct: 393 MTKLSSAQPFQGGFLLEKVMGPLSTGHLEL-KTRNPKDNPIVTFNYFQHPDDLKRCVRGI 452

Query: 423 RKVGDFLKTQTVENIKTRDLEGNKTIQFVGLPLPGNL---------SDDSAVGEFCKKTV 482
           + +   ++++     K  D+     +       P NL         S   +  EFC+ TV
Sbjct: 453 QTIERVVQSKAFSRYKYADVSFEYLLNLTA-STPVNLRPPRSGPGASLPPSAEEFCQHTV 512

Query: 483 TSYWHYHGGCLVGKVVNGNYSVIGVKNLRVLDGSTFAVSPGSNPTATLMMLAR 500
           T+ WHYHGGC+VG+VV+G+Y VIG+  LRV+D ST    PG+NP AT+MML R
Sbjct: 513 TTIWHYHGGCVVGRVVDGDYKVIGIDRLRVIDMSTVGYCPGTNPQATVMMLGR 558

BLAST of Chy5G099740 vs. TAIR 10
Match: AT5G51930.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 349.4 bits (895), Expect = 4.9e-96
Identity = 220/533 (41.28%), Postives = 307/533 (57.60%), Query Frame = 0

Query: 3   FVQDASDFPIKEEYDYIIVGGGTTGCPLAATLSKKFSVLLIERGSEPSKYPSVLNEQQLL 62
           F++DA+  P    +DYII+GGGT GC LAATLS+  SVL++ERG  P + P+  +    +
Sbjct: 60  FMKDATLAPKNASFDYIIIGGGTAGCALAATLSQNASVLVLERGGSPYENPTATDMGNSV 119

Query: 63  NVFTVEDDGKNPFNR-FISEDGVENIRGRVLGGSSMINGGVYSRADPEFFRTQLGMQELD 122
           N  T+ ++  N +++ FISEDGV N R RVLGG S+INGG YSRA  ++        E +
Sbjct: 120 N--TLLNNTPNSWSQLFISEDGVYNTRPRVLGGGSVINGGFYSRAGNDYVEE----AEWE 179

Query: 123 MEMVEKAYEWVEEAIVFKPSLNLWQGAFRRFLVEGGVEPDNGFDLRDDVGTKISGSIFDE 182
           ME VE AYEWVE+ +VF+P +  WQ AF+  L+E G  PDNGF      GTKI G+IFD 
Sbjct: 180 MEEVEAAYEWVEKKLVFEPQVIEWQKAFKDGLLEAGESPDNGFTYDHIYGTKIGGTIFDR 239

Query: 183 NGTRHGAVELLNKAQPTNLKVAVQAIVQRILFSGLSANGVLYSDSKGKLHTAIIRKK--G 242
            G RH A  LL  A P  + V + A V ++LF+   A  VL+ D+ G  H A +  K   
Sbjct: 240 AGHRHTAANLLEYANPNRIVVYLHASVHKVLFT-TEAYEVLFEDANGVFHKANLANKATN 299

Query: 243 EIIVSAGAIGSPQLLLLSGIGPKSYLSSLKL-PVVLHQPHVGQSMVDYPRFGYVLAWPFP 302
           E+I+SAGA+GSPQLL+LSG+GP  +L +  + P+VL QP VGQ M D P     +  P P
Sbjct: 300 EVILSAGALGSPQLLMLSGVGPAVHLEAHGVNPLVLDQPMVGQGMADNPMNFVAIPSPQP 359

Query: 303 LAFTSSKVIGISQNKTFYFQSIASTTPLSIPPLFSIFPP-----NSTSLTTT------SL 362
           +  +  + +GI++  + Y + ++  + LS       F       N TS TT+      S+
Sbjct: 360 VELSLIQAVGITKFDS-YIEGLSGLS-LSFDITRRFFDGVLNLLNETSHTTSRKILTQSI 419

Query: 363 ATI--------------GGKFSKV---ASTGSLRLNSSTDAASNPIVRFNYYSHPADVAM 422
           A +              G  F KV   AS G ++L  +T+   NP V FNYY  P D+  
Sbjct: 420 AVLLKSFDVKLEVRMNGGLIFQKVDGPASKGHMKLR-NTNPRDNPSVTFNYYQEPEDLNK 479

Query: 423 CVKGVRKVGDFLKTQTVENIKTRDLEGNKTIQ-FVGLPL---PGNLSDDSAVGEFCKKTV 482
           CVKG+  +   + ++     K   +   + +   + LP+   P +++    + +FC  TV
Sbjct: 480 CVKGLNTIIRMINSKAFSKYKYPGVTARELLNLMLALPINLRPRHVTSAFNLKQFCIDTV 539

Query: 483 TSYWHYHGGCLVGKVVNGNYSVIGVKNLRVLDGSTFAVSPGSNPTATLMMLAR 500
           TS WHYHGGC VGKVV+ NY V+G+  LRV+DGSTF  SPG+NP AT+MML R
Sbjct: 540 TSVWHYHGGCQVGKVVDKNYKVLGIDGLRVIDGSTFLKSPGTNPQATVMMLGR 582

BLAST of Chy5G099740 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 348.6 bits (893), Expect = 8.4e-96
Identity = 216/521 (41.46%), Postives = 290/521 (55.66%), Query Frame = 0

Query: 16  YDYIIVGGGTTGCPLAATLSKKFSVLLIERGSEPSKYPSVLNEQQLLNVFTVEDDGKNPF 75
           YDYI++GGGT GCPLAATLS+ FSVL++ERG  P    +V +  +  ++   +    +  
Sbjct: 63  YDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANV-SFLRNFHIGLADISASSAS 122

Query: 76  NRFISEDGVENIRGRVLGGSSMINGGVYSRADPEFFRTQLGMQELDMEMVEKAYEWVEEA 135
             F+S DGV N R RVLGG S IN G YSRAD  F +        D ++V+++Y WVE  
Sbjct: 123 QAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVK----RAGWDPKLVKESYPWVERE 182

Query: 136 IVFKPSLNLWQGAFRRFLVEGGVEPDNGFDLRDDVGTKISGSIFDENGTRHGAVELLNKA 195
           IV +P L LWQ A R  L+E GV P NGF      GTKI G+IFD  G RH A ELL  A
Sbjct: 183 IVHQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAELLAYA 242

Query: 196 QPTNLKVAVQAIVQRILF--SGL--SANGVLYSDSKGKLHTAII--RKKGEIIVSAGAIG 255
            P  L+V + A VQ+I+F  SG      GV++ D KG  H A++  RK  E+I+S+GAIG
Sbjct: 243 NPQKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSSGAIG 302

Query: 256 SPQLLLLSGIGPKSYLSSLKLPVVLHQPHVGQSMVDYPRFGYVLAWPFPLAFTSSKVIGI 315
           SPQ+L+LSGIGPK  L  LK+PVVL   HVG+ M D P    ++    P+  +  + +GI
Sbjct: 303 SPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPMNTILVPSKAPIEQSLIQTVGI 362

Query: 316 SQNKTFYFQSIA-STTPLSI----------PPLFSIFP-----PNSTSLTTT-------- 375
           ++   +   S     +P SI            LFS  P     P +T    T        
Sbjct: 363 TKMGVYVEASTGFGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITRNKYQLHE 422

Query: 376 --SLATIGGKFSKVASTGSLRLNSSTDAASNPIVRFNYYSHPADVAMCVKGVRKVGDFLK 435
             + + I  K +   S G L L  +T+   NP V FNY+ HP D+  CV+ +R V   + 
Sbjct: 423 AFNGSFILEKLAYPISRGHLSL-VNTNVDDNPSVTFNYFKHPVDLQRCVEAIRLVSKVVT 482

Query: 436 TQTVENIKTRDLEGNKTIQFVGLPL-----PGNLSDDSAVGEFCKKTVTSYWHYHGGCLV 495
           +    N    D +    +  + +       P  L+D  ++ +FCK TV + WHYHGGCLV
Sbjct: 483 SNRFLNYTQCDKQNVHKMLSLSVKANINLRPKQLNDTKSMAQFCKDTVVTIWHYHGGCLV 542

Query: 496 GKVVNGNYSVIGVKNLRVLDGSTFAVSPGSNPTATLMMLAR 500
           GKVV+ N  V+GV  LRV+DGSTF  SPG+NP AT+MM+ R
Sbjct: 543 GKVVSPNRKVLGVDRLRVIDGSTFDESPGTNPQATMMMMGR 577

BLAST of Chy5G099740 vs. TAIR 10
Match: AT5G51950.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 340.5 bits (872), Expect = 2.3e-93
Identity = 213/541 (39.37%), Postives = 300/541 (55.45%), Query Frame = 0

Query: 3   FVQDASDFPIKEEYDYIIVGGGTTGCPLAATLSKKFSVLLIERGSEPSKYPSVLNEQQLL 62
           F++DA+  P+   +DYII+GGGT+GC LAATLS+  SVL++ERG  P   P+  + +   
Sbjct: 33  FMKDATLAPMFARFDYIIIGGGTSGCALAATLSQNASVLVLERGGAPYDNPTATDIENFA 92

Query: 63  NVFTVEDDGKNPFNRFISEDGVENIRGRVLGGSSMINGGVYSRADPEFFRTQLGMQELDM 122
              +     K+    FISEDGV N R RVLGG S++N G Y+RA  E+ +      E   
Sbjct: 93  TTLS-NTSPKSWSQLFISEDGVYNTRARVLGGGSVLNAGFYTRAGDEYVKE----TEWKT 152

Query: 123 EMVEKAYEWVEEAIVFKPSLNLWQGAFRRFLVEGGVEPDNGFDLRDDVGTKISGSIFDEN 182
           + VE AYEWVE+ + F+P +  WQ AF+  L+E G  P NGF      GTKI G+IFD  
Sbjct: 153 DEVEAAYEWVEKKVAFQPPVLGWQTAFKDGLLEAGEFPYNGFTYDHIYGTKIGGTIFDRA 212

Query: 183 GTRHGAVELLNKAQPTNLKVAVQAIVQRILFSGL-----SANGVLYSDSKGKLHTAIIRK 242
           G RH A +LL  A P N+ V + A V +ILF+        A GV++ D+ G LH A + K
Sbjct: 213 GHRHTAADLLEYANPGNIVVYLHASVHKILFTTKGRPRPKAYGVIFQDANGVLHKAELEK 272

Query: 243 K--GEIIVSAGAIGSPQLLLLSGIGPKSYLSSLKL-PVVLHQPHVGQSMVDYPRFGYVLA 302
               E+I+SAGAIGSPQLL+LSGIGP ++L++  + P+VL  P VGQ M D P     + 
Sbjct: 273 NSMNEVILSAGAIGSPQLLMLSGIGPAAHLAAHGIKPLVLDHPMVGQGMGDNPMNAIFIP 332

Query: 303 WPFPLAFTSSKVIGISQNKTF----------------YFQSI-------------ASTTP 362
            P P+  +  +V+GI++ +++                +F  +             ++T+P
Sbjct: 333 SPTPVEVSLIQVVGITKFESYIEGASGVIFSYSWTRRFFDGVLNYLNEMQTSRTTSTTSP 392

Query: 363 -LSIPPLFSIFPP-NSTSLTTTSLATIGGKFSKVASTGSLRLNSSTDAASNPIVRFNYYS 422
            LS   +   F P N     TT    I  K +   S G L L  +T+   NP VRFNYY 
Sbjct: 393 TLSTQSITDFFNPINPLLNATTRAGLILQKIAGPISRGHLELR-NTNPDDNPSVRFNYYQ 452

Query: 423 HPADVAMCVKGVRKVGDFLKTQTVENIKTRDLEGNKTIQFVGLPLPGNLSDDSAVG---- 482
            P D+ +CV+G+  +   + ++     K  D   +  +  + L +P NL           
Sbjct: 453 EPEDLQICVEGINTIIKVINSKAFSKFKYPDATIHGLLDLM-LSVPTNLRPRHITSMFNL 512

Query: 483 -EFCKKTVTSYWHYHGGCLVGKVVNGNYSVIGVKNLRVLDGSTFAVSPGSNPTATLMMLA 500
            +FC  TV + WHYHGGC VG+VV+ NY V+G+ +LRV+DGSTF  SPG+NP AT+MML 
Sbjct: 513 RQFCIDTVMTIWHYHGGCQVGRVVDKNYRVLGIDSLRVIDGSTFLKSPGTNPQATVMMLG 566