Chy5G099710 (gene) Cucumber (hystrix) v1

Overview
NameChy5G099710
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Description(R)-mandelonitrile lyase 1-like
LocationchrH05: 13891602 .. 13899205 (+)
RNA-Seq ExpressionChy5G099710
SyntenyChy5G099710
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAATTATTGTAAGGCTACTATTCTCATACTCAGCCTTATCATCTCTATCTTTCAATTACAAGTTCACTCCTCGCATGCTATCCCCAATCAAGGTACTACGATTACAAATTGTTATAGAAAGTTATCGTCAGAAGAGGCTCACAGTTTTTCAACATTTGTAACATGTAGCCTTTTTTTTTTATTTCACATTTACCTTTGTCGATACTTATTTTGGTATGACTTTTCCTTTTTCAGATGTTAGCTACATGAAGTTTGTACATAATGCTACCGATTTACCAACAAAAGAAGAATATGACTACATAATTATAGGAGGAGGAACAGCAGGGTGTCCATTAGCTACAACATTATCATCAAAATTCTCAGTTCTCCTTCTTGAAAGAGGCAGTGACCCCAACATATATCCTTCTGTGTTGAATGAACAGGGTCTATCGAAGGCTTTTGATACAGAAGATGATGGCAAAACTCCCTTCCAACGGTTCATCTCGGAGGACGGCGTAGAGAACATTCGGGGGCGAGTCCTCGGCGGTGGAAGCATGGTCAATGCCGGGTTCTATTCAAGGGGACATAAAGAGTTTTTTGAAACTGCAGGTGTTGATTGGGACATGGAATTGGTGGAGAAAGCTTATGAATGGGTTGAAGAGACTGTGGTCTCACAACCGATTTTGAAAGATTGGCAATCTGCTTTTAGAACTGCTTTGTTGGAAGGTGGTATTGTTCCCGATAATGGGTTTGATTTGAGGCATCTTGTAGGTACTAAAACTGGAGGCTCTATCATCGATGATAAAGGAAATAGGCATGGAGCTGTGGAGCTTCTAAATAAGGCTAACCCCAAGAATCTTAAAGTTGCAATTGAAGCCACAGTCCAAAGAATCCTCTTCTCTGGTTAGTCCTAATTAAACATTTGGTTTACAAACATTTGTTTGTCTCCATTTCAATGTCTATCTTTTTTAAAAAGTAGCTAGGATTAGGTTAGAAATTATTTCAATTCTTCTAAGATTTTAAAACATGAATAAATTTAAAAGAATTGATTTTGACTAGGTTTTACCTCACGTGGGCAGGTATAATATGAAAAGTAGGCTACACTTGTTTACTTTCGTTATACACTGAGTTGTGGGATCCACCTTAAAATATACCATCAAACCATGCAATTTAATTGAGGATGAAACGGGTTGTCAATGGGTTGTCAATCTAATTGTAATTGAATTTTACGTTATGATAATTATCATTACATATATTTTGTTACTATACTAATTATGAATATGTCAAATTTGTTGTTTTAAGCGATAATGGTAATGATAATAATAACAATAAAATGTGTCAGATTCATAATTATAAGTAGACGTAATTAAAAGTAAAGTTAATTTTTATAAATATAACAAACCGACAAAATATTTACGATCCATGTAACAAAATGAAAAAGTCTATTAAGCTGACAATTTTTTTTAAATATTTCAGATTTTTCATTCATTTTCATCGTCTTTCTTCTTCTCTTCCTTTCTACGATTTTTTTCTTCTTTCCATCGTCTTTTTCTCGTTTTTAATGTTTTCATCGACAAATATAAAAGATCGTGAAAAATAATTTTTAGATATTGGATAACGAAACGATCGTGTACCAAAGAATCTTAAAAAAAATCATTTAGATTTGGCTACCCAAAGTTAAACGATCAAAATCTATGTAAAATCTAAATGATCGTGTACAAAAAAAAGATTTTTTTAGAAAACAATATAACTTGGGTCATTAGGTGCACTCAAGCATCACCACTAGGTGGGCACTAGGGGTGTAAGAATATGGGCTGAACTCGAATAACCCGGACTACCTAACCCATATTCTAAGGGTTGGGTTGCGTTAGGTTAAAATGTGGGTTGGGTTGAATTAAAAAAATTAATTTTTTTTTGGGTTGGGTTGAGTCTCGGGTTGGAGGGTTGAAAAATTCGGTTCAACCCAACCCAACCCAACCCAAATTAATATATATATATATATATATATATATATTTATATATTATTTATTTGATAAAATAAAATCTAAAAATTAGGTCTTCCTCCCTCTTCAATCTTCATCCCCTCGTGCCTCGCTCACCCTTCTTCACTGTGTTTGATTCTTCTCTTCCATCGCACATCAATCTTCAGTTTTCTCCTTCACAATCTCGCTGTCCACCTCCATTTCGTTAAAAAGTAGAAAAAAAAAAAAAAAAAAATAACAACACAATCCTACAACCCCCAACCCATAAAATATTGGCTGAGCTGGGTTAGGTTGGAAACTAAATTTGGGTTATTCGGGTTGTTAACCCATATAACTCAAAAATCTAGGTTGGGTTCTCCCAACCCAAGCGATTACATCCCTAGTGGACACCCTTTTAGCACCCTCATCATCTCCTATAAGATTTCATTCATTGAAGCACAAGGTACACAATATGTTAAAAAAAGAGAGACTAGAGATAAGCCCAAAGAAAGGAAGTTCAAGTAAAAGCATTACAATTTAGAGCAATAACAGTGGGGGAGTAGTCAGCGAAGAGTTTGTTTCCGGATGACCATTGGCCCCTGAAGTTGCAAATATTCTCCCAGATATTTGTTATGCTTACACAATTGTTGTTGAAAGTCCTGTTGTTTCGCTCCAACCAAATCGGCCATAAAGTAGCCACACCCATATTGAAAAATATGATACCTTTCTTATGATTTGTGTTTATCGAGCAGAGGTCTTTATAAAGGCTTTGACAGCTTTGGTTATTATTTTTCCAACCAGCATATTTCTTGATTTTCTCTCATAGAGAATTTGCAAAATTATAATGGATGAAGAGATGATCATCGAATTTCTTGATTTTCTCTCATAGAGAATTATACTAACCTGACAGAGGATACAACAATTACTAGGACTGATGCAATGGTTTTTTAGCCTACCTTGCAGCTTATCATAAGTGAATAATCCCTTTTGGAAGATAGACCACAAGAAGAATTTGCATTTCTTGGAATCTTTGATTTTAAAAGGGATTTGTATAGCAGAATATCCAGAGAATCAATAGTTGTGGCATCTAAATTTTGTTTAACAGAGTTTGCCGAAAATAAATCATTACCATTAATCGTTCAAATTGGAAAGTTTGGGCTCTATTTTGATTTGGCTAAGGGAGAGATGCTTTGATGGAGTTCCACAACTCTTGTTCTCTCTTAAGCAAAGGTCTTCTCGGCTTAAAGTCCCAGTCCTGAGTGGAATCATTCCAAATATCTTTGATGTTAGCATTTTGAGCTTGAGAAAGAGCAAAAAGTCTGGGGGCTGTCACTTTTAGGGGACCATTTTTACTTCACAGGTTGTGCTAGAAAGATAAGAGCTCTTCATTATTGATCTTCCAATCGATTATTTTAAAGCTATACCAGGCTGAACATTTGATAATTGATCTCCATAGAGCTTTCGTACTACTAAATTTTACTTCAGAAGGAAAAAGCTCAGATGGAGATTTGTTATGTTTGGCATCAATGAATTTCCTACATAAAGCAAGAGGTTCAGAACGATACCTCCAAAGCCATTTACACACATCAGAGCAAAGTTAGTATCAGCCATTTTAGTAATCCCGAGCCCTCTGTTTTTTGACATTGACACAATGGGCCATATGATTGGATGCAATTTCTTTGTTGTTTGCAAGGTAATATTGATAATCATGGCTACCTTTCCAGAGAAAATCCCTCCAATGTTTTTCAATCTTTTTGCAGACAGAAGATGGGGCTTTAAAGATCAACAATTGGTAAATGGGAAGGCTAGGTAATGTATAGTTGACGAAAGTGATTTTTCCCTCTATTGATACGTAAGAGTACTCCCAATTGTTTAGCCTTTTATGTATTTTCTCAGCAACATTGTCCCAAAAAGCCATGGAGCTAGGCTTTCCTTTTAAAGGCATCCCCAAGTAACTAATAGGCAGAAATTGGGTTGAAATCCCCCAACATTTGGCAGCAAAATCAACTCTTTCCTTGGAGACATTTATAGGAGAAATGACAGATTTTGATAAGTTGATATTGAGTCCAGAAGCTTCCTCAAATAAGTGGATTGCATATTGTAAATTTCTAAGGTAAAGATCATTATCTTCCACAAACATTAAAATGTCACCAGCAAAGAGGAGATGGGTAAGGTTGATATTGTTTATAGCAATACCTTTGATTTTGTTATTTTTTTTTTCAGATATATAAGGATCCGACTGAGATAATCCATAGCTATAACAAAAACAAAGGGAGAGATTGGATCACCTTGACAAATACCTCTACTTGGTTTGATTCTTCCATGAGGTTTGTCATTGATGAGGATAGAGTATTGTGTACCATTGAAACACAGGGCATAATCCACTCTCTCCATTTCATAGGAAAATGCTTTTTCATCACCATATAGTCAATAAATTTTCAGCTTATCTTATCAAAAGCCTTCTCAATATCAATCTTTATAACAAAGTCCTTGATTTTCTTGGCTCAACAGTAGTCAATAACTTTATTAGCAAGAAGCATAGCATCGGTAATCTGCATATCCTTTATGAAAGCCATTTGATTTTCAAAGATTGTATATGGTAATACAGCTTTGAGTTTAGTGGCCATGGCTTTTGCTATAATTTTGTATAAAGAAATAGCGAGGCTGGTGGGTTTGTAGTCAGTAGCGACTTTGCATTTCTCCTTCTTCGCAATTAGAGTGATATATGTTTCGTTCATATTTCTGTTGATCACCTGTTTTTGAAAAAATCATGGAATGCCTCCAATAAGTTTAGCTTGAAGATGTTCCTGAATTTTTTGTAAAACTCAATGGTAAAACTGTTCGGCCCTGGAGCTTTGTTCTTTTCAAATGAGCAAATGGAGTTTAAAATGTCTTTTTCAAAGAAGGGCCATCAGAAGAGTCTAAGCTTCTGTGCTGATGGGTTGTCATCAAGATTGTCAATGTATTGGGAGAATTTTCCATTGCTCTTATATATGTGCTAAAGTGATTTGTGAGGATCCTCTCAATGTCCTCATTTGTGTTGAAGCAAGTTTCGTTCTCATAAAAAATTTCAGAGATCATGTTTCTTCTCTGCCTTGCCGTACATATTTTGTGGAAAAAATTGGAGTTTTCATCACCTTCCATAAGCCAAAGCTTTTTTGCTCTTTGTGCTCAGTATTGAGCTTCTATAAGGATCAAACAACTAAGATTAGACTTTAATACTTTCCTTCTATCACTATCAGTTTCAGTCATCAACAGTTGATTTTTCAAGGCATCAATTTTGTCAATTTCGGCTCTCTTTTCTTGAAAAAATCTGTTGGTTCTTGGCTAGCAATCAGGGACTTGAAGGCGCTCTTTAGCCATTCCAGATCTCTTTAGGGTTTGTCGTGATTGAGAAGGACTTGTAGGGACAATATTTAGTGATCAGGAATCTGTTGTATCTTGATCTTTTGTCAATCGCCGAAGTGTATTGCTTTCTTCTAGTTTGGCAGTACCGCGGGAATATCATAGAGGAAGAGATCCTGAATTTTTTACAGTTAGTAACTTCCGGTAGTATCTGAAGGATGAAAAGGATGGGGTAATATATTGAAGGGGAAGATGTAAAGGTTGGTTAAAGAGATTTATGAATCTGCAGAGTTTGATTTGTAATGGTTGGCTGAGGTAAATGTTGATGGAGATAAATTATTAAGGGTTGTTTTGGCTGGACAAGTGTCAACATGGTGAGCTCGTTGGCGGCGAAAGGAAATTTGGAGGTCAAGAATGGGAAGTTGAGAGGGTTTTCTCTCTCATCTCTCTTCCAAGGAAACCAATCTCCCGAACCTCGTAAAAAAAATATTAAAAAAAAAACGCTATCTAATTTAAAGATCCACGATTAAATCTAAACGATCGTATATCGAAATAATCAAATCTATAGGATCGAAAGAATAGTTAAATGTAAACGATCGTTGGGTGTCAATTAATTCTGAGACATATTTTTGTATTTTTCATTATGAATTGTAGAGTTTTTTCTGTATTCAAAATTAAAAGATGCCTTAAAATTTCTGGACAAGTGTATTCAATTACACCCTTTTCCGATGTTTATCCATATCTGTCTCTTCACTCCCCTTTTTCACACATATATATCTCCGTTCTCTCCCCCATCTTTGCTCTTTTTTTATCTCCTTAACTTTTTTTCCTTTAATTAAAATTTTCTTTTAATACTCTATATGTGCTTTTTTTTTTCTTATCTTTTTTATGTTTTCATTTTTATTTCTACTTTTAGTACAGTATCATATAATTCTATTTCATAAAAAAAGTTATCCATCCTAGTAAATATTGTAAACATTTTATCAATAATAAACATTGATAGCTACTGATATACTTCTATTAGAGACATTGATAGATAGTGATAGAAGCCTATAAGTAGCTAGCATTGATAGAATTTAAAATTTTTATATAATTTATAAATATTTTAATTTGATTTGCTATTTTTAAAAATACCATTTAATTATTAGTAATAATTATCATTAGTCAGTATCAATAATGTTTAGCATTAATAATCAATAATATTCCTACTAATATATATAGTAACAAAAGTTTGTTATTACTTTTTTTCCCTTTTATCCCCATAATTTGTCTCTAGAACATTCATATTTATAATCTATATCCATATCAGCCATAATTAAAATGGTAGGATTTTTTTTCTTTAATCCATTATAAAAAAAATGTGATCAAACAATTTTAAATCAGTTTCAAATTAAGGGGTGCCCAAATAATGTATTAATTAACTCCACACTTTTTCAAATGTGTATTATATTATGAACATGCAGACTTATCAGCAAATGGAGTTTCATATTTAGATTCAACAGGAAAGTTACACACAGCATTCATTCATGAAAAAGGAGAAATATTTTTAAGTGCAGGAGCCATTGGAAGTCCTCAACTTCTCCTTCTAAGTGGGATTGGCCCAAAATCTTATCTTTCATCCTTAAAACTACCTGTCGTTCTTCACCAACCACATGTCGGACAATCCATGTCTGACAATCCCCGTTTTGGGACAAACATTGCTCTTCCATTCCAAGTGGTTCCTACATCAGGAAAAGTTGTTGGCATTTTACAAGACAATATCTATTTCCAATCTCTTGCTAGCCCTTCACCATTTTTAGTTCCACCAACTTTCAGTCTTCTTCCTCCTCATCCCACTTCAATTAATCCAACCTTAGCCACTTTTTTTGGGAAATTCTCGGAAGTTCATTCTAAAGGCTCACTTAAACTAAATTCCTCTATTGATGTGAAGAAGAGTCCCATTGTTCAATTCAATTATTATTCCCATCCTGATGATCTTGCTCGATGTGTTAAAGGTGTAAGAAAATTGGGAGATGTGTTTAAAACTCCAACAATGGAAAAGATTAAAACCCAAGATTTGAAGGGTAATAAAGGATTTATGTTTTTGGGGCTTCCATTGCCGGAAAATTTGTGGAATGATAGCGCTGTTGAAGAATATTGTAAGAAAACGGTGGCTACTTATTGGCATTATCATGGAGGATCTTTGGTCGGAAAAGTTGTCGACGGTAATTATAAAGTGATCGGAACTGAAAATTTACGTGTAGTTGATGGCTCCACTTTCTCTGTCTCGCCAGGAACTAATCCTATGGCCACTCTCATGATGCTTGGCCGGTGA

mRNA sequence

ATGGAGAATTATTGTAAGGCTACTATTCTCATACTCAGCCTTATCATCTCTATCTTTCAATTACAAGTTCACTCCTCGCATGCTATCCCCAATCAAGATGTTAGCTACATGAAGTTTGTACATAATGCTACCGATTTACCAACAAAAGAAGAATATGACTACATAATTATAGGAGGAGGAACAGCAGGGTGTCCATTAGCTACAACATTATCATCAAAATTCTCAGTTCTCCTTCTTGAAAGAGGCAGTGACCCCAACATATATCCTTCTGTGTTGAATGAACAGGGTCTATCGAAGGCTTTTGATACAGAAGATGATGGCAAAACTCCCTTCCAACGGTTCATCTCGGAGGACGGCGTAGAGAACATTCGGGGGCGAGTCCTCGGCGGTGGAAGCATGGTCAATGCCGGGTTCTATTCAAGGGGACATAAAGAGTTTTTTGAAACTGCAGGTGTTGATTGGGACATGGAATTGGTGGAGAAAGCTTATGAATGGGTTGAAGAGACTGTGGTCTCACAACCGATTTTGAAAGATTGGCAATCTGCTTTTAGAACTGCTTTGTTGGAAGGTGGTATTGTTCCCGATAATGGGTTTGATTTGAGGCATCTTGTAGGTACTAAAACTGGAGGCTCTATCATCGATGATAAAGGAAATAGGCATGGAGCTGTGGAGCTTCTAAATAAGGCTAACCCCAAGAATCTTAAAGTTGCAATTGAAGCCACAGTCCAAAGAATCCTCTTCTCTGACTTATCAGCAAATGGAGTTTCATATTTAGATTCAACAGGAAAGTTACACACAGCATTCATTCATGAAAAAGGAGAAATATTTTTAAGTGCAGGAGCCATTGGAAGTCCTCAACTTCTCCTTCTAAGTGGGATTGGCCCAAAATCTTATCTTTCATCCTTAAAACTACCTGTCGTTCTTCACCAACCACATGTCGGACAATCCATGTCTGACAATCCCCGTTTTGGGACAAACATTGCTCTTCCATTCCAAGTGGTTCCTACATCAGGAAAAGTTGTTGGCATTTTACAAGACAATATCTATTTCCAATCTCTTGCTAGCCCTTCACCATTTTTAGTTCCACCAACTTTCAGTCTTCTTCCTCCTCATCCCACTTCAATTAATCCAACCTTAGCCACTTTTTTTGGGAAATTCTCGGAAGTTCATTCTAAAGGCTCACTTAAACTAAATTCCTCTATTGATGTGAAGAAGAGTCCCATTGTTCAATTCAATTATTATTCCCATCCTGATGATCTTGCTCGATGTGTTAAAGGTGTAAGAAAATTGGGAGATGTGTTTAAAACTCCAACAATGGAAAAGATTAAAACCCAAGATTTGAAGGGTAATAAAGGATTTATGTTTTTGGGGCTTCCATTGCCGGAAAATTTGTGGAATGATAGCGCTGTTGAAGAATATTGTAAGAAAACGGTGGCTACTTATTGGCATTATCATGGAGGATCTTTGGTCGGAAAAGTTGTCGACGGTAATTATAAAGTGATCGGAACTGAAAATTTACGTGTAGTTGATGGCTCCACTTTCTCTGTCTCGCCAGGAACTAATCCTATGGCCACTCTCATGATGCTTGGCCGGTGA

Coding sequence (CDS)

ATGGAGAATTATTGTAAGGCTACTATTCTCATACTCAGCCTTATCATCTCTATCTTTCAATTACAAGTTCACTCCTCGCATGCTATCCCCAATCAAGATGTTAGCTACATGAAGTTTGTACATAATGCTACCGATTTACCAACAAAAGAAGAATATGACTACATAATTATAGGAGGAGGAACAGCAGGGTGTCCATTAGCTACAACATTATCATCAAAATTCTCAGTTCTCCTTCTTGAAAGAGGCAGTGACCCCAACATATATCCTTCTGTGTTGAATGAACAGGGTCTATCGAAGGCTTTTGATACAGAAGATGATGGCAAAACTCCCTTCCAACGGTTCATCTCGGAGGACGGCGTAGAGAACATTCGGGGGCGAGTCCTCGGCGGTGGAAGCATGGTCAATGCCGGGTTCTATTCAAGGGGACATAAAGAGTTTTTTGAAACTGCAGGTGTTGATTGGGACATGGAATTGGTGGAGAAAGCTTATGAATGGGTTGAAGAGACTGTGGTCTCACAACCGATTTTGAAAGATTGGCAATCTGCTTTTAGAACTGCTTTGTTGGAAGGTGGTATTGTTCCCGATAATGGGTTTGATTTGAGGCATCTTGTAGGTACTAAAACTGGAGGCTCTATCATCGATGATAAAGGAAATAGGCATGGAGCTGTGGAGCTTCTAAATAAGGCTAACCCCAAGAATCTTAAAGTTGCAATTGAAGCCACAGTCCAAAGAATCCTCTTCTCTGACTTATCAGCAAATGGAGTTTCATATTTAGATTCAACAGGAAAGTTACACACAGCATTCATTCATGAAAAAGGAGAAATATTTTTAAGTGCAGGAGCCATTGGAAGTCCTCAACTTCTCCTTCTAAGTGGGATTGGCCCAAAATCTTATCTTTCATCCTTAAAACTACCTGTCGTTCTTCACCAACCACATGTCGGACAATCCATGTCTGACAATCCCCGTTTTGGGACAAACATTGCTCTTCCATTCCAAGTGGTTCCTACATCAGGAAAAGTTGTTGGCATTTTACAAGACAATATCTATTTCCAATCTCTTGCTAGCCCTTCACCATTTTTAGTTCCACCAACTTTCAGTCTTCTTCCTCCTCATCCCACTTCAATTAATCCAACCTTAGCCACTTTTTTTGGGAAATTCTCGGAAGTTCATTCTAAAGGCTCACTTAAACTAAATTCCTCTATTGATGTGAAGAAGAGTCCCATTGTTCAATTCAATTATTATTCCCATCCTGATGATCTTGCTCGATGTGTTAAAGGTGTAAGAAAATTGGGAGATGTGTTTAAAACTCCAACAATGGAAAAGATTAAAACCCAAGATTTGAAGGGTAATAAAGGATTTATGTTTTTGGGGCTTCCATTGCCGGAAAATTTGTGGAATGATAGCGCTGTTGAAGAATATTGTAAGAAAACGGTGGCTACTTATTGGCATTATCATGGAGGATCTTTGGTCGGAAAAGTTGTCGACGGTAATTATAAAGTGATCGGAACTGAAAATTTACGTGTAGTTGATGGCTCCACTTTCTCTGTCTCGCCAGGAACTAATCCTATGGCCACTCTCATGATGCTTGGCCGGTGA

Protein sequence

MENYCKATILILSLIISIFQLQVHSSHAIPNQDVSYMKFVHNATDLPTKEEYDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNIYPSVLNEQGLSKAFDTEDDGKTPFQRFISEDGVENIRGRVLGGGSMVNAGFYSRGHKEFFETAGVDWDMELVEKAYEWVEETVVSQPILKDWQSAFRTALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKANPKNLKVAIEATVQRILFSDLSANGVSYLDSTGKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLPVVLHQPHVGQSMSDNPRFGTNIALPFQVVPTSGKVVGILQDNIYFQSLASPSPFLVPPTFSLLPPHPTSINPTLATFFGKFSEVHSKGSLKLNSSIDVKKSPIVQFNYYSHPDDLARCVKGVRKLGDVFKTPTMEKIKTQDLKGNKGFMFLGLPLPENLWNDSAVEEYCKKTVATYWHYHGGSLVGKVVDGNYKVIGTENLRVVDGSTFSVSPGTNPMATLMMLGR*
Homology
BLAST of Chy5G099710 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 546.6 bits (1407), Expect = 3.1e-154
Identity = 272/530 (51.32%), Postives = 364/530 (68.68%), Query Frame = 0

Query: 7   ATILILSLIISIFQL-QVHSSHAIPNQDVSYMKFVHNATDLPTKEEYDYIIIGGGTAGCP 66
           A +L+L L + + Q  +VHS     N D SY++F ++ATDL  +  YDY+I+GGGT+GCP
Sbjct: 8   AILLVLHLFVLLLQYSEVHSLATTSNHDFSYLRFAYDATDLELEGSYDYVIVGGGTSGCP 67

Query: 67  LATTLSSKFSVLLLERGSDPNIYPSVLNEQGLSKAFDTEDDGKTPFQRFISEDGVENIRG 126
           LA TLS K+ VL+LERGS P  YP+VL   G       EDDGKTP +RF+SEDG++N+RG
Sbjct: 68  LAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSEDGIDNVRG 127

Query: 127 RVLGGGSMVNAGFYSRGHKEFFETAGVDWDMELVEKAYEWVEETVVSQPILKDWQSAFRT 186
           RVLGG SM+NAG Y+R +   +  +GVDWDM+LV K YEWVE+T+V +P  + WQS   T
Sbjct: 128 RVLGGTSMINAGVYARANTSIYSASGVDWDMDLVNKTYEWVEDTIVFKPNYQPWQSVTGT 187

Query: 187 ALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKANPKNLKVAIEATVQRI 246
           A LE G+ P++GF L H  GT+  GS  D+KG RH A ELLNK N  NL+V + A+V++I
Sbjct: 188 AFLEAGVDPNHGFSLDHEAGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASVEKI 247

Query: 247 LFSD---LSANGVSYLDSTGKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSL 306
           +FS+   L+A GV Y DS G  H AF+  KGE+ +SAG IG+PQLLLLSG+GP+SYLSSL
Sbjct: 248 IFSNAPGLTATGVIYRDSNGTPHRAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYLSSL 307

Query: 307 KLPVVLHQPHVGQSMSDNPRFGTNIALPFQVVPTSGKVVGILQDNIYFQSLASPSPFLVP 366
            +PVVL  P+VGQ + DNPR   NI  P  + PT   V+GI  D  ++Q   S  PF  P
Sbjct: 308 NIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGISND--FYQCSFSSLPFTTP 367

Query: 367 PTFSLLPPHPTSI-NPTLATFFGKFSEVHSKGSLKLNSSIDVKKSPIVQFNYYSHPDDLA 426
           P FS  P     + N T A F  K +   S GSL L SS +V+ SP V+FNYYS+P DL+
Sbjct: 368 P-FSFFPSTSYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNPTDLS 427

Query: 427 RCVKGVRKLGDVFKTPTMEKIKTQDLKGNKGFMFLGLPLPENLWNDSAVEEYCKKTVATY 486
            CV G++K+G++  T  ++  K +DL G +GF  LG+PLP++  +D+A E +C+++VA+Y
Sbjct: 428 HCVSGMKKIGELLSTDALKPYKVEDLPGIEGFNILGIPLPKDQTDDAAFETFCRESVASY 487

Query: 487 WHYHGGSLVGKVVDGNYKVIGTENLRVVDGSTFSVSPGTNPMATLMMLGR 532
           WHYHGG LVGKV+DG+++V G + LRVVDGSTF  +P ++P    +MLGR
Sbjct: 488 WHYHGGCLVGKVLDGDFRVTGIDALRVVDGSTFPYTPASHPQGFYLMLGR 534

BLAST of Chy5G099710 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 1.2e-150
Identity = 268/535 (50.09%), Postives = 363/535 (67.85%), Query Frame = 0

Query: 6   KATILILSLIISIFQL-----QVHSSHAIPNQDVSYMKFVHNATDLPTKEEYDYIIIGGG 65
           K+T+  + L++ IF L     +VHS     + D SY+ F ++ATDL  +  YDY+I+GGG
Sbjct: 3   KSTMSAILLVLYIFVLHLQYSEVHSLATTSDHDFSYLSFAYDATDLELEGSYDYVIVGGG 62

Query: 66  TAGCPLATTLSSKFSVLLLERGSDPNIYPSVLNEQGLSKAFDTEDDGKTPFQRFISEDGV 125
           T+GCPLA TLS K+ VL+LERGS P  YP+VL   G       EDDGKTP +RF+SEDG+
Sbjct: 63  TSGCPLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSEDGI 122

Query: 126 ENIRGRVLGGGSMVNAGFYSRGHKEFFETAGVDWDMELVEKAYEWVEETVVSQPILKDWQ 185
           +N+RGRVLGG S++NAG Y+R +   +  +GVDWDM+LV + YEWVE+T+V +P  + WQ
Sbjct: 123 DNVRGRVLGGTSIINAGVYARANTSIYSASGVDWDMDLVNQTYEWVEDTIVYKPNSQSWQ 182

Query: 186 SAFRTALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKANPKNLKVAIEA 245
           S  +TA LE G+ P++GF L H  GT+  GS  D+KG RH A ELLNK N  NL+V + A
Sbjct: 183 SVTKTAFLEAGVHPNHGFSLDHEEGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHA 242

Query: 246 TVQRILFSD---LSANGVSYLDSTGKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPKS 305
           +V++I+FS+   L+A GV Y DS G  H AF+  KGE+ +SAG IG+PQLLLLSG+GP+S
Sbjct: 243 SVEKIIFSNAPGLTATGVIYRDSNGTPHQAFVRSKGEVIVSAGTIGTPQLLLLSGVGPES 302

Query: 306 YLSSLKLPVVLHQPHVGQSMSDNPRFGTNIALPFQVVPTSGKVVGILQDNIYFQSLASPS 365
           YLSSL +PVVL  P+VGQ + DNPR   NI  P  + PT   V+GI  D  ++Q   S  
Sbjct: 303 YLSSLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGISND--FYQCSFSSL 362

Query: 366 PFLVPPTFSLLPPHPTSI-NPTLATFFGKFSEVHSKGSLKLNSSIDVKKSPIVQFNYYSH 425
           PF  PP F   P     + N T A F  K +   S GSL L SS +V+ SP V+FNYYS+
Sbjct: 363 PFTTPP-FGFFPSASYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSN 422

Query: 426 PDDLARCVKGVRKLGDVFKTPTMEKIKTQDLKGNKGFMFLGLPLPENLWNDSAVEEYCKK 485
             DL+ CV G++K+G++  T  ++  K +DL G +GF  LG+PLP++  +D+A E +C++
Sbjct: 423 LTDLSHCVSGMKKIGELLSTDALKPYKVEDLPGVEGFNILGIPLPKDQTDDAAFETFCRE 482

Query: 486 TVATYWHYHGGSLVGKVVDGNYKVIGTENLRVVDGSTFSVSPGTNPMATLMMLGR 532
           +VA+YWHYHGG LVGKV+DG+++V G   LRVVDGSTF  +P ++P    +MLGR
Sbjct: 483 SVASYWHYHGGCLVGKVLDGDFRVTGINALRVVDGSTFPYTPASHPQGFYLMLGR 534

BLAST of Chy5G099710 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 4.9e-147
Identity = 267/536 (49.81%), Postives = 357/536 (66.60%), Query Frame = 0

Query: 6   KATILILSLIISIFQL-----QVHSSHAIPNQDVSYMKFVHNATDLPTKEEYDYIIIGGG 65
           K+T+  + L++ IF L     +V S     + D SY+ FV++ATD   +  YDYII+GGG
Sbjct: 3   KSTMSAVLLVLHIFVLHLQYSEVQSLANTSSHDFSYLSFVYDATDPELEGSYDYIIVGGG 62

Query: 66  TAGCPLATTLSSKFSVLLLERGSDPNIYPSVLNEQGLSKAFDTEDDGKTPFQRFISEDGV 125
           TAGCPLA TLS+ +SVL+LERGS P  YP++L   G       EDDGKTP +RF+SEDG+
Sbjct: 63  TAGCPLAATLSANYSVLVLERGSLPTEYPNLLISDGFVYNLQQEDDGKTPVERFVSEDGI 122

Query: 126 ENIRGRVLGGGSMVNAGFYSRGHKEFFETAGVDWDMELVEKAYEWVEETVVSQPILKDWQ 185
           +N+RGRVLGG SM+NAG Y R +  FF   G++WDM+LV + YEWVE+T+V +P  + WQ
Sbjct: 123 DNVRGRVLGGTSMINAGVYVRANTSFFNQTGIEWDMDLVNQTYEWVEDTIVFEPDSQTWQ 182

Query: 186 SAFRTALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKANPKNLKVAIEA 245
           +   TA LE GI+P+NGF + HL GT+  GS  D+ G RH + ELLNK +P NL+VA++A
Sbjct: 183 TVIGTAYLEAGILPNNGFSVDHLAGTRLTGSTFDNNGTRHASDELLNKGDPNNLRVAVQA 242

Query: 246 TVQRILFSD----LSANGVSYLDSTGKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPK 305
            V++I+FS     ++A GV Y DS G  H AF+  +GE+ LSAG IGSPQLLLLSG+GP+
Sbjct: 243 AVEKIIFSSNTSGVTAIGVIYTDSNGTTHQAFVRGEGEVILSAGPIGSPQLLLLSGVGPE 302

Query: 306 SYLSSLKLPVVLHQPHVGQSMSDNPRFGTNIALPFQVVPTSGKVVGILQDNIYFQSLASP 365
           SYL+SL + VV   P+VGQ + DNPR   NI  P  +  ++  V+GI  D  ++Q   S 
Sbjct: 303 SYLTSLNISVVASHPYVGQYIYDNPRNFINILPPNPIEASTVTVLGITSD--FYQCSISS 362

Query: 366 SPFLVPPTFSLLPPHPTSI-NPTLATFFGKFSEVHSKGSLKLNSSIDVKKSPIVQFNYYS 425
            PF  PP FS  P     + N T A    K     S G++ LNSS DV+  P V+FNYYS
Sbjct: 363 LPFDTPP-FSFFPTTSYPLPNQTFAHIVNKVPGPLSHGTVTLNSSSDVRVGPNVKFNYYS 422

Query: 426 HPDDLARCVKGVRKLGDVFKTPTMEKIKTQDLKGNKGFMFLGLPLPENLWNDSAVEEYCK 485
           +  DL+ CV G++KLG+V  T  +E  K +DL G  GF  LG+PLPEN  +D+A E +C+
Sbjct: 423 NLTDLSHCVSGMKKLGEVLSTDALEPYKVEDLPGIDGFNILGIPLPENQTDDAAFETFCR 482

Query: 486 KTVATYWHYHGGSLVGKVVDGNYKVIGTENLRVVDGSTFSVSPGTNPMATLMMLGR 532
           ++VA+YWHYHGG LVGKV+D  ++V G   LRVVDGSTF  +P ++P    +MLGR
Sbjct: 483 ESVASYWHYHGGCLVGKVLDDGFRVTGINALRVVDGSTFPSTPASHPQGFYLMLGR 535

BLAST of Chy5G099710 vs. ExPASy Swiss-Prot
Match: O82784 ((R)-mandelonitrile lyase 4 OS=Prunus serotina OX=23207 GN=MDL4 PE=2 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 1.2e-145
Identity = 263/534 (49.25%), Postives = 354/534 (66.29%), Query Frame = 0

Query: 7   ATILILSLIISIFQLQVHSSHAIPN----QDVSYMKFVHNATDLPTKEEYDYIIIGGGTA 66
           A +L+L+L+  +  LQ    H++ N     D  Y+KFV+NA DL  +  YDYII+GGGT+
Sbjct: 8   AVVLVLNLL--VLHLQYSEVHSLANTSSEHDFGYLKFVYNAVDLELEGSYDYIIVGGGTS 67

Query: 67  GCPLATTLSSKFSVLLLERGSDPNIYPSVLNEQGLSKAFDTEDDGKTPFQRFISEDGVEN 126
           GCPLA TLS+ +SVL+LERG+    YP+ L   G +     +DDGKTP +RF+SEDG++N
Sbjct: 68  GCPLAATLSANYSVLVLERGTIATEYPNTLTVDGFAYNLQQQDDGKTPVERFVSEDGIDN 127

Query: 127 IRGRVLGGGSMVNAGFYSRGHKEFFETAGVDWDMELVEKAYEWVEETVVSQPILKDWQSA 186
           +R R+LGG +++NAG Y+R ++ F+  +GV+WD++LV +AYEWVE+ +V +P  + WQS 
Sbjct: 128 VRSRILGGTTIINAGVYARANESFYNNSGVEWDLDLVNEAYEWVEDAIVYKPSNQSWQSI 187

Query: 187 FRTALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKANPKNLKVAIEATV 246
             TA LE G+ PDNGF L H  GT+  GS  D+ G RH + ELLNK +P NLKVA+EA V
Sbjct: 188 TGTAFLEAGVHPDNGFGLVHEEGTRLTGSTFDNSGTRHASDELLNKGDPDNLKVAVEAAV 247

Query: 247 QRILF----SDLSANGVSYLDSTGKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPKSY 306
           Q+I+F    S L+A GV Y DS G  H A +  KGE+ LSAG +G+PQLLLLSG+GP+SY
Sbjct: 248 QKIIFSTESSGLTAVGVVYTDSNGTSHRALVSGKGEVILSAGTLGTPQLLLLSGVGPESY 307

Query: 307 LSSLKLPVVLHQPHVGQSMSDNPRFGTNIALPFQVVPTSGKVVGILQDNIYFQSLASPSP 366
           L+SL + VV   P+VGQ ++DNPR   NI  P  + P++  V+GI  D  ++Q   S  P
Sbjct: 308 LTSLNISVVASHPYVGQYVNDNPRNFINILPPNPIEPSTVTVLGITSD--FYQCSLSSLP 367

Query: 367 FLVPPTFSLLPPHPTSI-NPTLATFFGKFSEVHSKGSLKLNSSIDVKKSPIVQFNYYSHP 426
           F  PP FSL P     + N T A    K     S GSL L SS +V  +P V+FNY S P
Sbjct: 368 FDTPP-FSLFPTTSYPLPNQTFAHIVSKVPGPLSAGSLTLQSSSNVSVAPNVKFNYCSDP 427

Query: 427 DDLARCVKGVRKLGDVFKTPTMEKIKTQDLKGNKGFMFLGLPLPENLWNDSAVEEYCKKT 486
            DL  CV G++K+G    T  ++  K  DL G  GF  LG PLPEN  +D+A E++C+ T
Sbjct: 428 VDLTHCVSGMKKIGVFLSTDALKPYKVDDLPGIDGFNILGTPLPENQTDDAAFEKFCRDT 487

Query: 487 VATYWHYHGGSLVGKVVDGNYKVIGTENLRVVDGSTFSVSPGTNPMATLMMLGR 532
           VA+YWHYHGG++VGKV+DGN++V G   LRVVDGSTF  +P ++P    +MLGR
Sbjct: 488 VASYWHYHGGAIVGKVIDGNFRVTGINALRVVDGSTFPATPASHPQGFYLMLGR 536

BLAST of Chy5G099710 vs. ExPASy Swiss-Prot
Match: O24243 ((R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 1.5e-143
Identity = 258/532 (48.50%), Postives = 351/532 (65.98%), Query Frame = 0

Query: 8   TILILSLIISIFQLQVHSSHAIPN---QDVSYMKFVHNATDLPTKEEYDYIIIGGGTAGC 67
           ++++  L + +  LQ    H++ N    D SY+KFV+NATD   +  YDYI+IGGGT+GC
Sbjct: 7   SVILFVLHLLVLHLQYSEVHSLANTSAHDFSYLKFVYNATDTSLEGSYDYIVIGGGTSGC 66

Query: 68  PLATTLSSKFSVLLLERGSDPNIYPSVLNEQGLSKAFDTEDDGKTPFQRFISEDGVENIR 127
           PLA TLS K+ VLLLERG+    YP+ L   G +     +DDGKTP +RF+SEDG++N+R
Sbjct: 67  PLAATLSEKYKVLLLERGTIATEYPNTLTADGFAYNLQQQDDGKTPVERFVSEDGIDNVR 126

Query: 128 GRVLGGGSMVNAGFYSRGHKEFFETAGVDWDMELVEKAYEWVEETVVSQPILKDWQSAFR 187
            R+LGG +++NAG Y+R +  F+   G++WD++LV K YEWVE+ +V +P  + WQS   
Sbjct: 127 ARILGGTTIINAGVYARANISFYSQTGIEWDLDLVNKTYEWVEDAIVVKPNNQSWQSVIG 186

Query: 188 TALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKANPKNLKVAIEATVQR 247
              LE GI+PDNGF L H  GT+  GS  D+ G RH A ELLNK +P NL VA++A+V++
Sbjct: 187 EGFLEAGILPDNGFSLDHEAGTRLTGSTFDNNGTRHAADELLNKGDPNNLLVAVQASVEK 246

Query: 248 ILF----SDLSANGVSYLDSTGKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPKSYLS 307
           ILF    S+LSA GV Y DS G  H AF+   GE+ +SAG IG+PQLLLLSG+GP+SYLS
Sbjct: 247 ILFSSNTSNLSAIGVIYTDSDGNSHQAFVRGNGEVIVSAGTIGTPQLLLLSGVGPESYLS 306

Query: 308 SLKLPVVLHQPHVGQSMSDNPRFGTNIALPFQVVPTSGKVVGILQDNIYFQSLASPSPFL 367
           SL + VV   P+VGQ + +NPR   N   P  +  +   V+GI  D  Y+Q   S  PF 
Sbjct: 307 SLNITVVQPNPYVGQFLYNNPRNFINNFPPNPIEASVVTVLGIRSD--YYQVSLSSLPFS 366

Query: 368 VPPTFSLLPPHPTSI-NPTLATFFGKFSEVHSKGSLKLNSSIDVKKSPIVQFNYYSHPDD 427
            PP FSL P     + N T A    +     S GS+ LNSS DV+ +P ++FNYYS+  D
Sbjct: 367 TPP-FSLFPTTSYPLPNSTFAHIVSQVPGPLSHGSVTLNSSSDVRIAPNIKFNYYSNSTD 426

Query: 428 LARCVKGVRKLGDVFKTPTMEKIKTQDLKGNKGFMFLGLPLPENLWNDSAVEEYCKKTVA 487
           LA CV G++KLGD+ +T  +E  K +D+ G  GF +LG+PLPEN  +D++ E +C   VA
Sbjct: 427 LANCVSGMKKLGDLLRTKALEPYKARDVLGIDGFNYLGVPLPENQTDDASFETFCLDNVA 486

Query: 488 TYWHYHGGSLVGKVVDGNYKVIGTENLRVVDGSTFSVSPGTNPMATLMMLGR 532
           +YWHYHGGSLVGKV+D +++V+G + LRVVD STF   P ++P    +MLGR
Sbjct: 487 SYWHYHGGSLVGKVLDDSFRVMGIKALRVVDASTFPYEPNSHPQGFYLMLGR 535

BLAST of Chy5G099710 vs. ExPASy TrEMBL
Match: A0A0A0LZU8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G627390 PE=3 SV=1)

HSP 1 Score: 1024.2 bits (2647), Expect = 1.9e-295
Identity = 505/531 (95.10%), Postives = 518/531 (97.55%), Query Frame = 0

Query: 1   MENYCKATILILSLIISIFQLQVHSSHAIPNQDVSYMKFVHNATDLPTKEEYDYIIIGGG 60
           MENY KA ILIL+LIISIFQLQVHSSHAIPNQDVSYMKFVHNATDLP KEE+DYIIIGGG
Sbjct: 1   MENYSKANILILNLIISIFQLQVHSSHAIPNQDVSYMKFVHNATDLPRKEEFDYIIIGGG 60

Query: 61  TAGCPLATTLSSKFSVLLLERGSDPNIYPSVLNEQGLSKAFDTEDDGKTPFQRFISEDGV 120
           TAGCPLATTLSSKFSVLLLERGSDPN YPSVLNEQGL KAFDTEDDGKTPFQ F+SEDGV
Sbjct: 61  TAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLKAFDTEDDGKTPFQHFVSEDGV 120

Query: 121 ENIRGRVLGGGSMVNAGFYSRGHKEFFETAGVDWDMELVEKAYEWVEETVVSQPILKDWQ 180
           ENIRGRVLGGGSMVNAGFYSR HKEFFE+AGVDWDMELVEKAYEWVEE+VVSQPILKDWQ
Sbjct: 121 ENIRGRVLGGGSMVNAGFYSRAHKEFFESAGVDWDMELVEKAYEWVEESVVSQPILKDWQ 180

Query: 181 SAFRTALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKANPKNLKVAIEA 240
           SAFR+ALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKA+PKNLKVAIEA
Sbjct: 181 SAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKADPKNLKVAIEA 240

Query: 241 TVQRILFSDLSANGVSYLDSTGKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPKSYLS 300
           TV+RILFSDLSANGVSYLDS GKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPKS+LS
Sbjct: 241 TVRRILFSDLSANGVSYLDSKGKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPKSHLS 300

Query: 301 SLKLPVVLHQPHVGQSMSDNPRFGTNIALPFQVVPTSGKVVGILQDNIYFQSLASPSPFL 360
           SLKLPVVLHQPHVGQSM+DNPRFGTNI LPFQVVPTSGKVVGILQDNIYFQS+ASPSPFL
Sbjct: 301 SLKLPVVLHQPHVGQSMTDNPRFGTNIILPFQVVPTSGKVVGILQDNIYFQSIASPSPFL 360

Query: 361 VPPTFSLLPPHPTSINPTLATFFGKFSEVHSKGSLKLNSSIDVKKSPIVQFNYYSHPDDL 420
           VPPTFSLLPPHPTSINPTLA FFGKFSEVHSKGSLKLNSSIDVKKSPIVQFNYYSHPDDL
Sbjct: 361 VPPTFSLLPPHPTSINPTLAIFFGKFSEVHSKGSLKLNSSIDVKKSPIVQFNYYSHPDDL 420

Query: 421 ARCVKGVRKLGDVFKTPTMEKIKTQDLKGNKGFMFLGLPLPENLWNDSAVEEYCKKTVAT 480
           ARCVKGVRKLGDVFKTPT+EKIKTQ+LKGNKGFMFLGLPLP NLWNDSAVEEYCKKTVAT
Sbjct: 421 ARCVKGVRKLGDVFKTPTLEKIKTQNLKGNKGFMFLGLPLPGNLWNDSAVEEYCKKTVAT 480

Query: 481 YWHYHGGSLVGKVVDGNYKVIGTENLRVVDGSTFSVSPGTNPMATLMMLGR 532
           YWHYHGG LVGKVVDGNYKVI TENLRVVDGSTFSVSPGTNPMATLMMLGR
Sbjct: 481 YWHYHGGCLVGKVVDGNYKVIETENLRVVDGSTFSVSPGTNPMATLMMLGR 531

BLAST of Chy5G099710 vs. ExPASy TrEMBL
Match: A0A1S3BSR3 ((R)-mandelonitrile lyase 1-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493130 PE=3 SV=1)

HSP 1 Score: 981.1 bits (2535), Expect = 1.8e-282
Identity = 486/531 (91.53%), Postives = 504/531 (94.92%), Query Frame = 0

Query: 1   MENYCKATILILSLIISIFQLQVHSSHAIPNQDVSYMKFVHNATDLPTKEEYDYIIIGGG 60
           MENY K+TILILSLIISIFQLQVHSSHAIPNQ+VSYMKFVHN  DLP KE+YDYIIIGGG
Sbjct: 1   MENYSKSTILILSLIISIFQLQVHSSHAIPNQNVSYMKFVHNGIDLPAKEKYDYIIIGGG 60

Query: 61  TAGCPLATTLSSKFSVLLLERGSDPNIYPSVLNEQGLSKAFDTEDDGKTPFQRFISEDGV 120
           TAGCPLATTLSSKFSVLLLERGSDPN YP VLNEQGLSKAF TEDDGKTPFQRF+SEDGV
Sbjct: 61  TAGCPLATTLSSKFSVLLLERGSDPNKYPYVLNEQGLSKAFGTEDDGKTPFQRFVSEDGV 120

Query: 121 ENIRGRVLGGGSMVNAGFYSRGHKEFFETAGVDWDMELVEKAYEWVEETVVSQPILKDWQ 180
           ENIRGRVLGGGSMVN GFYSRGHKEFFE+AGVDWDMELVEKAYEWVEETVVSQPILKDWQ
Sbjct: 121 ENIRGRVLGGGSMVNIGFYSRGHKEFFESAGVDWDMELVEKAYEWVEETVVSQPILKDWQ 180

Query: 181 SAFRTALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKANPKNLKVAIEA 240
           +AFR+ALLEGGIVPDNGFDLRHLVGTKTGGSI D+KGNRHGAVELLNKANPKNLKVAIE 
Sbjct: 181 AAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPKNLKVAIEV 240

Query: 241 TVQRILFSDLSANGVSYLDSTGKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPKSYLS 300
           TVQRILFSDLSA GVSYLDSTGKLHTAFIH+KGEI LSAGAIGSPQLLLLSGIGPKS+LS
Sbjct: 241 TVQRILFSDLSAKGVSYLDSTGKLHTAFIHKKGEIVLSAGAIGSPQLLLLSGIGPKSHLS 300

Query: 301 SLKLPVVLHQPHVGQSMSDNPRFGTNIALPFQVVPTSGKVVGILQDNIYFQSLASPSPFL 360
           SLKLPVVLHQP+VG+SMSDNPRFG ++ + FQVVPT+ KVVGILQDNIYFQSLA PSPFL
Sbjct: 301 SLKLPVVLHQPYVGKSMSDNPRFGASMVVQFQVVPTAAKVVGILQDNIYFQSLAGPSPFL 360

Query: 361 VPPTFSLLPPHPTSINPTLATFFGKFSEVHSKGSLKLNSSIDVKKSPIVQFNYYSHPDDL 420
           VPPTFSLLP  PTSIN TLA F GKFSEV+SKGSLKLNSSIDVKKSPIVQFNYYSHP DL
Sbjct: 361 VPPTFSLLPSQPTSINLTLANFVGKFSEVNSKGSLKLNSSIDVKKSPIVQFNYYSHPGDL 420

Query: 421 ARCVKGVRKLGDVFKTPTMEKIKTQDLKGNKGFMFLGLPLPENLWNDSAVEEYCKKTVAT 480
           ARCVK VRKLGDVFKTPTMEKIKTQDL+GNK FMFLGLPLPENLWNDSAVEEYCKKTVAT
Sbjct: 421 ARCVKSVRKLGDVFKTPTMEKIKTQDLEGNKRFMFLGLPLPENLWNDSAVEEYCKKTVAT 480

Query: 481 YWHYHGGSLVGKVVDGNYKVIGTENLRVVDGSTFSVSPGTNPMATLMMLGR 532
           YWHYHGG LVGKVVDGNYKVIG ENLRVVDGSTFSVSPGTNPMATLMMLGR
Sbjct: 481 YWHYHGGCLVGKVVDGNYKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGR 531

BLAST of Chy5G099710 vs. ExPASy TrEMBL
Match: A0A1S3BSS8 ((R)-mandelonitrile lyase 1-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103493130 PE=3 SV=1)

HSP 1 Score: 926.8 bits (2394), Expect = 4.1e-266
Identity = 456/499 (91.38%), Postives = 473/499 (94.79%), Query Frame = 0

Query: 33  DVSYMKFVHNATDLPTKEEYDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNIYPSVL 92
           +VSYMKFVHN  DLP KE+YDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPN YP VL
Sbjct: 33  NVSYMKFVHNGIDLPAKEKYDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPYVL 92

Query: 93  NEQGLSKAFDTEDDGKTPFQRFISEDGVENIRGRVLGGGSMVNAGFYSRGHKEFFETAGV 152
           NEQGLSKAF TEDDGKTPFQRF+SEDGVENIRGRVLGGGSMVN GFYSRGHKEFFE+AGV
Sbjct: 93  NEQGLSKAFGTEDDGKTPFQRFVSEDGVENIRGRVLGGGSMVNIGFYSRGHKEFFESAGV 152

Query: 153 DWDMELVEKAYEWVEETVVSQPILKDWQSAFRTALLEGGIVPDNGFDLRHLVGTKTGGSI 212
           DWDMELVEKAYEWVEETVVSQPILKDWQ+AFR+ALLEGGIVPDNGFDLRHLVGTKTGGSI
Sbjct: 153 DWDMELVEKAYEWVEETVVSQPILKDWQAAFRSALLEGGIVPDNGFDLRHLVGTKTGGSI 212

Query: 213 IDDKGNRHGAVELLNKANPKNLKVAIEATVQRILFSDLSANGVSYLDSTGKLHTAFIHEK 272
            D+KGNRHGAVELLNKANPKNLKVAIE TVQRILFSDLSA GVSYLDSTGKLHTAFIH+K
Sbjct: 213 FDNKGNRHGAVELLNKANPKNLKVAIEVTVQRILFSDLSAKGVSYLDSTGKLHTAFIHKK 272

Query: 273 GEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLPVVLHQPHVGQSMSDNPRFGTNIALPFQ 332
           GEI LSAGAIGSPQLLLLSGIGPKS+LSSLKLPVVLHQP+VG+SMSDNPRFG ++ + FQ
Sbjct: 273 GEIVLSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVGKSMSDNPRFGASMVVQFQ 332

Query: 333 VVPTSGKVVGILQDNIYFQSLASPSPFLVPPTFSLLPPHPTSINPTLATFFGKFSEVHSK 392
           VVPT+ KVVGILQDNIYFQSLA PSPFLVPPTFSLLP  PTSIN TLA F GKFSEV+SK
Sbjct: 333 VVPTAAKVVGILQDNIYFQSLAGPSPFLVPPTFSLLPSQPTSINLTLANFVGKFSEVNSK 392

Query: 393 GSLKLNSSIDVKKSPIVQFNYYSHPDDLARCVKGVRKLGDVFKTPTMEKIKTQDLKGNKG 452
           GSLKLNSSIDVKKSPIVQFNYYSHP DLARCVK VRKLGDVFKTPTMEKIKTQDL+GNK 
Sbjct: 393 GSLKLNSSIDVKKSPIVQFNYYSHPGDLARCVKSVRKLGDVFKTPTMEKIKTQDLEGNKR 452

Query: 453 FMFLGLPLPENLWNDSAVEEYCKKTVATYWHYHGGSLVGKVVDGNYKVIGTENLRVVDGS 512
           FMFLGLPLPENLWNDSAVEEYCKKTVATYWHYHGG LVGKVVDGNYKVIG ENLRVVDGS
Sbjct: 453 FMFLGLPLPENLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIGIENLRVVDGS 512

Query: 513 TFSVSPGTNPMATLMMLGR 532
           TFSVSPGTNPMATLMMLGR
Sbjct: 513 TFSVSPGTNPMATLMMLGR 531

BLAST of Chy5G099710 vs. ExPASy TrEMBL
Match: A0A5A7VHS6 ((R)-mandelonitrile lyase 1-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold231G00760 PE=3 SV=1)

HSP 1 Score: 920.6 bits (2378), Expect = 3.0e-264
Identity = 453/495 (91.52%), Postives = 469/495 (94.75%), Query Frame = 0

Query: 37  MKFVHNATDLPTKEEYDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNIYPSVLNEQG 96
           MKFVHN  DLP KE+YDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPN YP VLNEQG
Sbjct: 1   MKFVHNGIDLPAKEKYDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPYVLNEQG 60

Query: 97  LSKAFDTEDDGKTPFQRFISEDGVENIRGRVLGGGSMVNAGFYSRGHKEFFETAGVDWDM 156
           LSKAF TEDDGKTPFQRF+SEDGVENIRGRVLGGGSMVN GFYSRGHKEFFE+AGVDWDM
Sbjct: 61  LSKAFGTEDDGKTPFQRFVSEDGVENIRGRVLGGGSMVNIGFYSRGHKEFFESAGVDWDM 120

Query: 157 ELVEKAYEWVEETVVSQPILKDWQSAFRTALLEGGIVPDNGFDLRHLVGTKTGGSIIDDK 216
           ELVEKAYEWVEETVVSQPILKDWQ+AFR+ALLEGGIVPDNGFDLRHLVGTKTGGSI D+K
Sbjct: 121 ELVEKAYEWVEETVVSQPILKDWQAAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFDNK 180

Query: 217 GNRHGAVELLNKANPKNLKVAIEATVQRILFSDLSANGVSYLDSTGKLHTAFIHEKGEIF 276
           GNRHGAVELLNKANPKNLKVAIE TVQRILFSDLSA GVSYLDSTGKLHTAFIH+KGEI 
Sbjct: 181 GNRHGAVELLNKANPKNLKVAIEVTVQRILFSDLSAKGVSYLDSTGKLHTAFIHKKGEIV 240

Query: 277 LSAGAIGSPQLLLLSGIGPKSYLSSLKLPVVLHQPHVGQSMSDNPRFGTNIALPFQVVPT 336
           LSAGAIGSPQLLLLSGIGPKS+LSSLKLPVVLHQP+VG+SMSDNPRFG ++ + FQVVPT
Sbjct: 241 LSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVGKSMSDNPRFGASMVVQFQVVPT 300

Query: 337 SGKVVGILQDNIYFQSLASPSPFLVPPTFSLLPPHPTSINPTLATFFGKFSEVHSKGSLK 396
           + KVVGILQDNIYFQSLA PSPFLVPPTFSLLP  PTSIN TLA F GKFSEV+SKGSLK
Sbjct: 301 AAKVVGILQDNIYFQSLAGPSPFLVPPTFSLLPSQPTSINLTLANFVGKFSEVNSKGSLK 360

Query: 397 LNSSIDVKKSPIVQFNYYSHPDDLARCVKGVRKLGDVFKTPTMEKIKTQDLKGNKGFMFL 456
           LNSSIDVKKSPIVQFNYYSHP DLARCVK VRKLGDVFKTPTMEKIKTQDL+GNK FMFL
Sbjct: 361 LNSSIDVKKSPIVQFNYYSHPGDLARCVKSVRKLGDVFKTPTMEKIKTQDLEGNKRFMFL 420

Query: 457 GLPLPENLWNDSAVEEYCKKTVATYWHYHGGSLVGKVVDGNYKVIGTENLRVVDGSTFSV 516
           GLPLPENLWNDSAVEEYCKKTVATYWHYHGG LVGKVVDGNYKVIG ENLRVVDGSTFSV
Sbjct: 421 GLPLPENLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIGIENLRVVDGSTFSV 480

Query: 517 SPGTNPMATLMMLGR 532
           SPGTNPMATLMMLGR
Sbjct: 481 SPGTNPMATLMMLGR 495

BLAST of Chy5G099710 vs. ExPASy TrEMBL
Match: A0A1S3BTY8 ((R)-mandelonitrile lyase 1-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC103493130 PE=3 SV=1)

HSP 1 Score: 920.6 bits (2378), Expect = 3.0e-264
Identity = 453/495 (91.52%), Postives = 469/495 (94.75%), Query Frame = 0

Query: 37  MKFVHNATDLPTKEEYDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNIYPSVLNEQG 96
           MKFVHN  DLP KE+YDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPN YP VLNEQG
Sbjct: 1   MKFVHNGIDLPAKEKYDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPYVLNEQG 60

Query: 97  LSKAFDTEDDGKTPFQRFISEDGVENIRGRVLGGGSMVNAGFYSRGHKEFFETAGVDWDM 156
           LSKAF TEDDGKTPFQRF+SEDGVENIRGRVLGGGSMVN GFYSRGHKEFFE+AGVDWDM
Sbjct: 61  LSKAFGTEDDGKTPFQRFVSEDGVENIRGRVLGGGSMVNIGFYSRGHKEFFESAGVDWDM 120

Query: 157 ELVEKAYEWVEETVVSQPILKDWQSAFRTALLEGGIVPDNGFDLRHLVGTKTGGSIIDDK 216
           ELVEKAYEWVEETVVSQPILKDWQ+AFR+ALLEGGIVPDNGFDLRHLVGTKTGGSI D+K
Sbjct: 121 ELVEKAYEWVEETVVSQPILKDWQAAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFDNK 180

Query: 217 GNRHGAVELLNKANPKNLKVAIEATVQRILFSDLSANGVSYLDSTGKLHTAFIHEKGEIF 276
           GNRHGAVELLNKANPKNLKVAIE TVQRILFSDLSA GVSYLDSTGKLHTAFIH+KGEI 
Sbjct: 181 GNRHGAVELLNKANPKNLKVAIEVTVQRILFSDLSAKGVSYLDSTGKLHTAFIHKKGEIV 240

Query: 277 LSAGAIGSPQLLLLSGIGPKSYLSSLKLPVVLHQPHVGQSMSDNPRFGTNIALPFQVVPT 336
           LSAGAIGSPQLLLLSGIGPKS+LSSLKLPVVLHQP+VG+SMSDNPRFG ++ + FQVVPT
Sbjct: 241 LSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVGKSMSDNPRFGASMVVQFQVVPT 300

Query: 337 SGKVVGILQDNIYFQSLASPSPFLVPPTFSLLPPHPTSINPTLATFFGKFSEVHSKGSLK 396
           + KVVGILQDNIYFQSLA PSPFLVPPTFSLLP  PTSIN TLA F GKFSEV+SKGSLK
Sbjct: 301 AAKVVGILQDNIYFQSLAGPSPFLVPPTFSLLPSQPTSINLTLANFVGKFSEVNSKGSLK 360

Query: 397 LNSSIDVKKSPIVQFNYYSHPDDLARCVKGVRKLGDVFKTPTMEKIKTQDLKGNKGFMFL 456
           LNSSIDVKKSPIVQFNYYSHP DLARCVK VRKLGDVFKTPTMEKIKTQDL+GNK FMFL
Sbjct: 361 LNSSIDVKKSPIVQFNYYSHPGDLARCVKSVRKLGDVFKTPTMEKIKTQDLEGNKRFMFL 420

Query: 457 GLPLPENLWNDSAVEEYCKKTVATYWHYHGGSLVGKVVDGNYKVIGTENLRVVDGSTFSV 516
           GLPLPENLWNDSAVEEYCKKTVATYWHYHGG LVGKVVDGNYKVIG ENLRVVDGSTFSV
Sbjct: 421 GLPLPENLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIGIENLRVVDGSTFSV 480

Query: 517 SPGTNPMATLMMLGR 532
           SPGTNPMATLMMLGR
Sbjct: 481 SPGTNPMATLMMLGR 495

BLAST of Chy5G099710 vs. NCBI nr
Match: XP_011650118.2 ((R)-mandelonitrile lyase 1-like [Cucumis sativus] >KGN61134.2 hypothetical protein Csa_021306 [Cucumis sativus])

HSP 1 Score: 1026 bits (2654), Expect = 0.0
Identity = 509/531 (95.86%), Postives = 521/531 (98.12%), Query Frame = 0

Query: 1   MENYCKATILILSLIISIFQLQVHSSHAIPNQDVSYMKFVHNATDLPTKEEYDYIIIGGG 60
           MENY KATILI+SLIISIFQLQVH SHAIPNQDVSYMKFVHNATDLPTKEEYDYIIIGGG
Sbjct: 1   MENYSKATILIVSLIISIFQLQVHFSHAIPNQDVSYMKFVHNATDLPTKEEYDYIIIGGG 60

Query: 61  TAGCPLATTLSSKFSVLLLERGSDPNIYPSVLNEQGLSKAFDTEDDGKTPFQRFISEDGV 120
           TAGCPLATTLSSKFSVLLLERGSDPN YPSVLNE+GLSKAFDTEDDGKTPFQRF+SEDGV
Sbjct: 61  TAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEKGLSKAFDTEDDGKTPFQRFVSEDGV 120

Query: 121 ENIRGRVLGGGSMVNAGFYSRGHKEFFETAGVDWDMELVEKAYEWVEETVVSQPILKDWQ 180
           ENIRGR+LGGGSMVNAGFYSRGHKEFFE+AGVDWDMELVEKAYEW+EE+VVSQPILKDWQ
Sbjct: 121 ENIRGRILGGGSMVNAGFYSRGHKEFFESAGVDWDMELVEKAYEWIEESVVSQPILKDWQ 180

Query: 181 SAFRTALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKANPKNLKVAIEA 240
           SAFR+ALLEGGIVPDNGFDL HLVGTKTGGSIIDDKGNRHGAVELLNKANPKNLKVAIEA
Sbjct: 181 SAFRSALLEGGIVPDNGFDLSHLVGTKTGGSIIDDKGNRHGAVELLNKANPKNLKVAIEA 240

Query: 241 TVQRILFSDLSANGVSYLDSTGKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPKSYLS 300
           TVQRILFSDLSANGVSYLDS GKLHT FIHEKGEI +SAGAIGSPQLLLLSGIGPKS+LS
Sbjct: 241 TVQRILFSDLSANGVSYLDSKGKLHTTFIHEKGEIIVSAGAIGSPQLLLLSGIGPKSHLS 300

Query: 301 SLKLPVVLHQPHVGQSMSDNPRFGTNIALPFQVVPTSGKVVGILQDNIYFQSLASPSPFL 360
           SLKLPVVLHQPHVGQSMSDNPRFGTNI LPFQVVPTSGKVVGILQDNIYFQSLASPSPFL
Sbjct: 301 SLKLPVVLHQPHVGQSMSDNPRFGTNIVLPFQVVPTSGKVVGILQDNIYFQSLASPSPFL 360

Query: 361 VPPTFSLLPPHPTSINPTLATFFGKFSEVHSKGSLKLNSSIDVKKSPIVQFNYYSHPDDL 420
           VPPTFSLLPPHPTSINPTLATFFGKFSEVHSKGSLKLNSSIDVKKSPIVQFNYYSHPDDL
Sbjct: 361 VPPTFSLLPPHPTSINPTLATFFGKFSEVHSKGSLKLNSSIDVKKSPIVQFNYYSHPDDL 420

Query: 421 ARCVKGVRKLGDVFKTPTMEKIKTQDLKGNKGFMFLGLPLPENLWNDSAVEEYCKKTVAT 480
           ARCVKGVRKLGDVFKTPTMEKIKTQDLKGNKGFMFLGLPLP NLWNDSAVEEYCKKTVAT
Sbjct: 421 ARCVKGVRKLGDVFKTPTMEKIKTQDLKGNKGFMFLGLPLPGNLWNDSAVEEYCKKTVAT 480

Query: 481 YWHYHGGSLVGKVVDGNYKVIGTENLRVVDGSTFSVSPGTNPMATLMMLGR 531
           YWHYHGG LVGKVVDGNYKVIGTENLRVVDGSTFS+SPGTNPMAT+MMLGR
Sbjct: 481 YWHYHGGCLVGKVVDGNYKVIGTENLRVVDGSTFSISPGTNPMATVMMLGR 531

BLAST of Chy5G099710 vs. NCBI nr
Match: XP_031736583.1 ((R)-mandelonitrile lyase 1 [Cucumis sativus] >KAE8651658.1 hypothetical protein Csa_021303 [Cucumis sativus])

HSP 1 Score: 1011 bits (2613), Expect = 0.0
Identity = 503/531 (94.73%), Postives = 518/531 (97.55%), Query Frame = 0

Query: 1   MENYCKATILILSLIISIFQLQVHSSHAIPNQDVSYMKFVHNATDLPTKEEYDYIIIGGG 60
           MENY KA ILIL+LIISIFQLQVHSSHAIPNQDVSYMKFVHNATDLP KEE+DYIIIGGG
Sbjct: 1   MENYSKANILILNLIISIFQLQVHSSHAIPNQDVSYMKFVHNATDLPRKEEFDYIIIGGG 60

Query: 61  TAGCPLATTLSSKFSVLLLERGSDPNIYPSVLNEQGLSKAFDTEDDGKTPFQRFISEDGV 120
           TAGCPLATTLSSKFSVLLLERGSDPN YPSVLNEQGL KAFDTEDDGKTPFQ F+SEDGV
Sbjct: 61  TAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLKAFDTEDDGKTPFQHFVSEDGV 120

Query: 121 ENIRGRVLGGGSMVNAGFYSRGHKEFFETAGVDWDMELVEKAYEWVEETVVSQPILKDWQ 180
           ENIRGRVLGGGSMVNAGFYSR HKEFFE+AGVDWDMELVEKAYEWVEE+VVSQPILKDWQ
Sbjct: 121 ENIRGRVLGGGSMVNAGFYSRAHKEFFESAGVDWDMELVEKAYEWVEESVVSQPILKDWQ 180

Query: 181 SAFRTALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKANPKNLKVAIEA 240
           SAFR+ALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKA+PKNLKVAIEA
Sbjct: 181 SAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKADPKNLKVAIEA 240

Query: 241 TVQRILFSDLSANGVSYLDSTGKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPKSYLS 300
           TV+RILFSDLSANGVSYLDS GKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPKS+LS
Sbjct: 241 TVRRILFSDLSANGVSYLDSKGKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPKSHLS 300

Query: 301 SLKLPVVLHQPHVGQSMSDNPRFGTNIALPFQVVPTSGKVVGILQDNIYFQSLASPSPFL 360
           SLKLPVVLHQPHVGQSM+DNPRFGTNI LPFQVVPTSGKVVGILQDNIYFQS+ASPSPFL
Sbjct: 301 SLKLPVVLHQPHVGQSMTDNPRFGTNIILPFQVVPTSGKVVGILQDNIYFQSIASPSPFL 360

Query: 361 VPPTFSLLPPHPTSINPTLATFFGKFSEVHSKGSLKLNSSIDVKKSPIVQFNYYSHPDDL 420
           VPPTFSLLPPHPTSINPTLA FFGKFSEVHSKGSLKLNSSIDVKK+PIV+FNYYSHPDDL
Sbjct: 361 VPPTFSLLPPHPTSINPTLAIFFGKFSEVHSKGSLKLNSSIDVKKNPIVRFNYYSHPDDL 420

Query: 421 ARCVKGVRKLGDVFKTPTMEKIKTQDLKGNKGFMFLGLPLPENLWNDSAVEEYCKKTVAT 480
           ARCVKGVRKLGDVFKTPT+EKIKTQ+LKGNKGFMFLGLPLP NLWNDSAVEEYCKKTVAT
Sbjct: 421 ARCVKGVRKLGDVFKTPTLEKIKTQNLKGNKGFMFLGLPLPGNLWNDSAVEEYCKKTVAT 480

Query: 481 YWHYHGGSLVGKVVDGNYKVIGTENLRVVDGSTFSVSPGTNPMATLMMLGR 531
           YWHYHGG LVGKVVDGNYKVI TENLRVVDGSTFSVSPGTNPMATLMMLGR
Sbjct: 481 YWHYHGGCLVGKVVDGNYKVIETENLRVVDGSTFSVSPGTNPMATLMMLGR 531

BLAST of Chy5G099710 vs. NCBI nr
Match: XP_008451990.1 (PREDICTED: (R)-mandelonitrile lyase 1-like isoform X1 [Cucumis melo])

HSP 1 Score: 970 bits (2508), Expect = 0.0
Identity = 486/531 (91.53%), Postives = 504/531 (94.92%), Query Frame = 0

Query: 1   MENYCKATILILSLIISIFQLQVHSSHAIPNQDVSYMKFVHNATDLPTKEEYDYIIIGGG 60
           MENY K+TILILSLIISIFQLQVHSSHAIPNQ+VSYMKFVHN  DLP KE+YDYIIIGGG
Sbjct: 1   MENYSKSTILILSLIISIFQLQVHSSHAIPNQNVSYMKFVHNGIDLPAKEKYDYIIIGGG 60

Query: 61  TAGCPLATTLSSKFSVLLLERGSDPNIYPSVLNEQGLSKAFDTEDDGKTPFQRFISEDGV 120
           TAGCPLATTLSSKFSVLLLERGSDPN YP VLNEQGLSKAF TEDDGKTPFQRF+SEDGV
Sbjct: 61  TAGCPLATTLSSKFSVLLLERGSDPNKYPYVLNEQGLSKAFGTEDDGKTPFQRFVSEDGV 120

Query: 121 ENIRGRVLGGGSMVNAGFYSRGHKEFFETAGVDWDMELVEKAYEWVEETVVSQPILKDWQ 180
           ENIRGRVLGGGSMVN GFYSRGHKEFFE+AGVDWDMELVEKAYEWVEETVVSQPILKDWQ
Sbjct: 121 ENIRGRVLGGGSMVNIGFYSRGHKEFFESAGVDWDMELVEKAYEWVEETVVSQPILKDWQ 180

Query: 181 SAFRTALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKANPKNLKVAIEA 240
           +AFR+ALLEGGIVPDNGFDLRHLVGTKTGGSI D+KGNRHGAVELLNKANPKNLKVAIE 
Sbjct: 181 AAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPKNLKVAIEV 240

Query: 241 TVQRILFSDLSANGVSYLDSTGKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPKSYLS 300
           TVQRILFSDLSA GVSYLDSTGKLHTAFIH+KGEI LSAGAIGSPQLLLLSGIGPKS+LS
Sbjct: 241 TVQRILFSDLSAKGVSYLDSTGKLHTAFIHKKGEIVLSAGAIGSPQLLLLSGIGPKSHLS 300

Query: 301 SLKLPVVLHQPHVGQSMSDNPRFGTNIALPFQVVPTSGKVVGILQDNIYFQSLASPSPFL 360
           SLKLPVVLHQP+VG+SMSDNPRFG ++ + FQVVPT+ KVVGILQDNIYFQSLA PSPFL
Sbjct: 301 SLKLPVVLHQPYVGKSMSDNPRFGASMVVQFQVVPTAAKVVGILQDNIYFQSLAGPSPFL 360

Query: 361 VPPTFSLLPPHPTSINPTLATFFGKFSEVHSKGSLKLNSSIDVKKSPIVQFNYYSHPDDL 420
           VPPTFSLLP  PTSIN TLA F GKFSEV+SKGSLKLNSSIDVKKSPIVQFNYYSHP DL
Sbjct: 361 VPPTFSLLPSQPTSINLTLANFVGKFSEVNSKGSLKLNSSIDVKKSPIVQFNYYSHPGDL 420

Query: 421 ARCVKGVRKLGDVFKTPTMEKIKTQDLKGNKGFMFLGLPLPENLWNDSAVEEYCKKTVAT 480
           ARCVK VRKLGDVFKTPTMEKIKTQDL+GNK FMFLGLPLPENLWNDSAVEEYCKKTVAT
Sbjct: 421 ARCVKSVRKLGDVFKTPTMEKIKTQDLEGNKRFMFLGLPLPENLWNDSAVEEYCKKTVAT 480

Query: 481 YWHYHGGSLVGKVVDGNYKVIGTENLRVVDGSTFSVSPGTNPMATLMMLGR 531
           YWHYHGG LVGKVVDGNYKVIG ENLRVVDGSTFSVSPGTNPMATLMMLGR
Sbjct: 481 YWHYHGGCLVGKVVDGNYKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGR 531

BLAST of Chy5G099710 vs. NCBI nr
Match: XP_008452005.2 (PREDICTED: (R)-mandelonitrile lyase 1-like isoform X2 [Cucumis melo])

HSP 1 Score: 917 bits (2369), Expect = 0.0
Identity = 456/499 (91.38%), Postives = 473/499 (94.79%), Query Frame = 0

Query: 33  DVSYMKFVHNATDLPTKEEYDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNIYPSVL 92
           +VSYMKFVHN  DLP KE+YDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPN YP VL
Sbjct: 33  NVSYMKFVHNGIDLPAKEKYDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPYVL 92

Query: 93  NEQGLSKAFDTEDDGKTPFQRFISEDGVENIRGRVLGGGSMVNAGFYSRGHKEFFETAGV 152
           NEQGLSKAF TEDDGKTPFQRF+SEDGVENIRGRVLGGGSMVN GFYSRGHKEFFE+AGV
Sbjct: 93  NEQGLSKAFGTEDDGKTPFQRFVSEDGVENIRGRVLGGGSMVNIGFYSRGHKEFFESAGV 152

Query: 153 DWDMELVEKAYEWVEETVVSQPILKDWQSAFRTALLEGGIVPDNGFDLRHLVGTKTGGSI 212
           DWDMELVEKAYEWVEETVVSQPILKDWQ+AFR+ALLEGGIVPDNGFDLRHLVGTKTGGSI
Sbjct: 153 DWDMELVEKAYEWVEETVVSQPILKDWQAAFRSALLEGGIVPDNGFDLRHLVGTKTGGSI 212

Query: 213 IDDKGNRHGAVELLNKANPKNLKVAIEATVQRILFSDLSANGVSYLDSTGKLHTAFIHEK 272
            D+KGNRHGAVELLNKANPKNLKVAIE TVQRILFSDLSA GVSYLDSTGKLHTAFIH+K
Sbjct: 213 FDNKGNRHGAVELLNKANPKNLKVAIEVTVQRILFSDLSAKGVSYLDSTGKLHTAFIHKK 272

Query: 273 GEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLPVVLHQPHVGQSMSDNPRFGTNIALPFQ 332
           GEI LSAGAIGSPQLLLLSGIGPKS+LSSLKLPVVLHQP+VG+SMSDNPRFG ++ + FQ
Sbjct: 273 GEIVLSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVGKSMSDNPRFGASMVVQFQ 332

Query: 333 VVPTSGKVVGILQDNIYFQSLASPSPFLVPPTFSLLPPHPTSINPTLATFFGKFSEVHSK 392
           VVPT+ KVVGILQDNIYFQSLA PSPFLVPPTFSLLP  PTSIN TLA F GKFSEV+SK
Sbjct: 333 VVPTAAKVVGILQDNIYFQSLAGPSPFLVPPTFSLLPSQPTSINLTLANFVGKFSEVNSK 392

Query: 393 GSLKLNSSIDVKKSPIVQFNYYSHPDDLARCVKGVRKLGDVFKTPTMEKIKTQDLKGNKG 452
           GSLKLNSSIDVKKSPIVQFNYYSHP DLARCVK VRKLGDVFKTPTMEKIKTQDL+GNK 
Sbjct: 393 GSLKLNSSIDVKKSPIVQFNYYSHPGDLARCVKSVRKLGDVFKTPTMEKIKTQDLEGNKR 452

Query: 453 FMFLGLPLPENLWNDSAVEEYCKKTVATYWHYHGGSLVGKVVDGNYKVIGTENLRVVDGS 512
           FMFLGLPLPENLWNDSAVEEYCKKTVATYWHYHGG LVGKVVDGNYKVIG ENLRVVDGS
Sbjct: 453 FMFLGLPLPENLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIGIENLRVVDGS 512

Query: 513 TFSVSPGTNPMATLMMLGR 531
           TFSVSPGTNPMATLMMLGR
Sbjct: 513 TFSVSPGTNPMATLMMLGR 531

BLAST of Chy5G099710 vs. NCBI nr
Match: XP_008451997.1 (PREDICTED: (R)-mandelonitrile lyase 1-like isoform X3 [Cucumis melo])

HSP 1 Score: 911 bits (2354), Expect = 0.0
Identity = 453/495 (91.52%), Postives = 469/495 (94.75%), Query Frame = 0

Query: 37  MKFVHNATDLPTKEEYDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNIYPSVLNEQG 96
           MKFVHN  DLP KE+YDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPN YP VLNEQG
Sbjct: 1   MKFVHNGIDLPAKEKYDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPYVLNEQG 60

Query: 97  LSKAFDTEDDGKTPFQRFISEDGVENIRGRVLGGGSMVNAGFYSRGHKEFFETAGVDWDM 156
           LSKAF TEDDGKTPFQRF+SEDGVENIRGRVLGGGSMVN GFYSRGHKEFFE+AGVDWDM
Sbjct: 61  LSKAFGTEDDGKTPFQRFVSEDGVENIRGRVLGGGSMVNIGFYSRGHKEFFESAGVDWDM 120

Query: 157 ELVEKAYEWVEETVVSQPILKDWQSAFRTALLEGGIVPDNGFDLRHLVGTKTGGSIIDDK 216
           ELVEKAYEWVEETVVSQPILKDWQ+AFR+ALLEGGIVPDNGFDLRHLVGTKTGGSI D+K
Sbjct: 121 ELVEKAYEWVEETVVSQPILKDWQAAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFDNK 180

Query: 217 GNRHGAVELLNKANPKNLKVAIEATVQRILFSDLSANGVSYLDSTGKLHTAFIHEKGEIF 276
           GNRHGAVELLNKANPKNLKVAIE TVQRILFSDLSA GVSYLDSTGKLHTAFIH+KGEI 
Sbjct: 181 GNRHGAVELLNKANPKNLKVAIEVTVQRILFSDLSAKGVSYLDSTGKLHTAFIHKKGEIV 240

Query: 277 LSAGAIGSPQLLLLSGIGPKSYLSSLKLPVVLHQPHVGQSMSDNPRFGTNIALPFQVVPT 336
           LSAGAIGSPQLLLLSGIGPKS+LSSLKLPVVLHQP+VG+SMSDNPRFG ++ + FQVVPT
Sbjct: 241 LSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVGKSMSDNPRFGASMVVQFQVVPT 300

Query: 337 SGKVVGILQDNIYFQSLASPSPFLVPPTFSLLPPHPTSINPTLATFFGKFSEVHSKGSLK 396
           + KVVGILQDNIYFQSLA PSPFLVPPTFSLLP  PTSIN TLA F GKFSEV+SKGSLK
Sbjct: 301 AAKVVGILQDNIYFQSLAGPSPFLVPPTFSLLPSQPTSINLTLANFVGKFSEVNSKGSLK 360

Query: 397 LNSSIDVKKSPIVQFNYYSHPDDLARCVKGVRKLGDVFKTPTMEKIKTQDLKGNKGFMFL 456
           LNSSIDVKKSPIVQFNYYSHP DLARCVK VRKLGDVFKTPTMEKIKTQDL+GNK FMFL
Sbjct: 361 LNSSIDVKKSPIVQFNYYSHPGDLARCVKSVRKLGDVFKTPTMEKIKTQDLEGNKRFMFL 420

Query: 457 GLPLPENLWNDSAVEEYCKKTVATYWHYHGGSLVGKVVDGNYKVIGTENLRVVDGSTFSV 516
           GLPLPENLWNDSAVEEYCKKTVATYWHYHGG LVGKVVDGNYKVIG ENLRVVDGSTFSV
Sbjct: 421 GLPLPENLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIGIENLRVVDGSTFSV 480

Query: 517 SPGTNPMATLMMLGR 531
           SPGTNPMATLMMLGR
Sbjct: 481 SPGTNPMATLMMLGR 495

BLAST of Chy5G099710 vs. TAIR 10
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 506.5 bits (1303), Expect = 2.6e-143
Identity = 259/537 (48.23%), Postives = 355/537 (66.11%), Query Frame = 0

Query: 7   ATILILSLIISIFQLQVHSSHAIP--NQDVSYMKFVHNATDLPTKEEYDYIIIGGGTAGC 66
           +++L  +L++ +    VH S+A P  N+   +M+F+ NATD  +++ YDYII+GGGTAGC
Sbjct: 7   SSLLYTALVVLLLLGVVHRSNARPRVNRPPGFMRFISNATDFASEDYYDYIIVGGGTAGC 66

Query: 67  PLATTLSSKFSVLLLERGSDPNIYPSVLNEQGLSKAFDTEDDGKTPFQRFISEDGVENIR 126
           PLA TLS  F VLLLERG  P   P+V++  G        ++  +P Q FISE+GV N R
Sbjct: 67  PLAATLSQSFRVLLLERGGVPYNRPNVMSHDGFLTTLTDVNNFDSPAQSFISEEGVPNAR 126

Query: 127 GRVLGGGSMVNAGFYSRGHKEFFETAGVDWDMELVEKAYEWVEETVVSQPILKDWQSAFR 186
           GRVLGG S +NAGFYSR  K+FFE +G+ WD+  V ++YEWVE  +V +P L+ WQ+A R
Sbjct: 127 GRVLGGSSAINAGFYSRADKQFFENSGLVWDLSSVNQSYEWVERAIVFRPQLRTWQTAIR 186

Query: 187 TALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKANPKNLKVAIEATVQR 246
            ALLE G+ P NGF L H VGTK GGS  D  G RH + +LL  A   N++VA+ ATV+R
Sbjct: 187 DALLEVGVHPFNGFTLEHKVGTKIGGSTFDRTGRRHSSADLLRYARSSNIRVAVYATVER 246

Query: 247 ILF--------SDLSANGVSYLDSTGKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPK 306
           +L         S++SA GV Y D  G+ H A I ++GE+ LSAGA+GSPQLL LSGIGP+
Sbjct: 247 VLLASSPSVSGSNVSAIGVVYRDQLGRFHHALIRDRGEVILSAGALGSPQLLFLSGIGPR 306

Query: 307 SYLSSLKLPVVLHQPHVGQSMSDNPRFGTNIALPFQVVPTSGKVVGILQDNIYFQSLASP 366
           SYLS+  +PV L QPHVG  + DNPR G +I  P  +  +  +VVG+ +D  + ++ ++ 
Sbjct: 307 SYLSTWGIPVALDQPHVGDFVYDNPRNGISIVPPVPMENSLIQVVGVTEDGAFLEAASNV 366

Query: 367 SPFLVP--PTFSLLPPHPTSINPTLATFFGKFSEVHSKGSLKLNSSIDVKKSPIVQFNYY 426
            PF  P    F   P  P  +  T  T   K     S G L+L +S DV+ +P+V+FNY+
Sbjct: 367 IPFASPLHSVFIRAPASPLYVPVT--TIMEKILGPVSIGLLRL-ASTDVRINPVVRFNYF 426

Query: 427 SHPDDLARCVKGVRKLGDVFKTPTMEKIKTQDLKGNKGFMFLGLPLPENLWNDSAVEEYC 486
           S P DL RCV G RK+G++ ++  M+    ++  GN+ F F+G PLP +  ND  + ++C
Sbjct: 427 SDPQDLERCVNGTRKIGEILRSRAMQDFMIREWFGNRRFRFVGAPLPVDQSNDLVMADFC 486

Query: 487 KKTVATYWHYHGGSLVGKVVDGNYKVIGTENLRVVDGSTFSVSPGTNPMATLMMLGR 532
           ++TV+T WHYHGG++VGKVVD + KVIG  +LR+VDGSTF++SPGTNP ATLMMLGR
Sbjct: 487 RRTVSTIWHYHGGAVVGKVVDSDLKVIGVNSLRLVDGSTFNISPGTNPQATLMMLGR 540

BLAST of Chy5G099710 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 400.2 bits (1027), Expect = 2.6e-111
Identity = 238/559 (42.58%), Postives = 309/559 (55.28%), Query Frame = 0

Query: 12  LSLIISIF-QLQVHSSHAIPNQDVSYMKFVHNATDLPTKEEYDYIIIGGGTAGCPLATTL 71
           L + +S+F    + SS   PN       F+ +AT  PT   YDYIIIGGGTAGCPLA TL
Sbjct: 10  LFVALSLFLHSPICSSDKAPN-----YSFMRDATGSPTTSYYDYIIIGGGTAGCPLAATL 69

Query: 72  SSKFSVLLLERGSDPNIYPSVLNEQGLSKAFDTEDDGKTPFQRFISEDGVENIRGRVLGG 131
           S   SVLLLERG  P   P++        A     +  +P QRF+SEDGV N R RVLGG
Sbjct: 70  SQNASVLLLERGDSPYNNPNITRLSAFGAALSDLSE-SSPSQRFVSEDGVINARARVLGG 129

Query: 132 GSMVNAGFYSRGHKEFFETAGVDWDMELVEKAYEWVEETVVSQPILKDWQSAFRTALLEG 191
           GS +NAGFY+R   ++    G  WD  L  ++Y+WVE  V  QP +  WQ+A R  LLE 
Sbjct: 130 GSALNAGFYTRAGTKYVRNMG--WDGALANESYQWVEAKVAFQPPMGRWQTAVRDGLLEA 189

Query: 192 GIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKANPKNLKVAIEATVQRILFSDL 251
           GIVP+NGF   H+ GTK GG+I D  GNRH A +LL  A+PK + V + ATV RILF   
Sbjct: 190 GIVPNNGFTYDHINGTKFGGTIFDRNGNRHTAADLLEYADPKGITVLLHATVHRILFRTR 249

Query: 252 S-----ANGVSYLDSTGKLHTAFIHE--KGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLK 311
                 ANGV Y D TG+ H A++ E    EI LSAG +GSPQLL+LSG+GP + L +  
Sbjct: 250 GTTKPIANGVVYRDRTGQAHRAYLKEGALSEIILSAGTLGSPQLLMLSGVGPSAQLQAQN 309

Query: 312 LPVVLHQPHVGQSMSDNPRFGTNIALPFQVVPTSGKVVGILQDNIYFQSL---------- 371
           + VV+ QPHVGQ M DNP     +  P  V  +  +VVGI  +  Y ++           
Sbjct: 310 ITVVMDQPHVGQGMYDNPMNAVFVPSPVPVEVSLIEVVGITGEGTYVEAAGGENFGGGGG 369

Query: 372 ASPSPFLVPPTFSLLPPHPTSINPTLAT------------FFGKFSEVHSKGSLKLNSSI 431
            S         +++  P  T +     T               K     S G L+L +  
Sbjct: 370 GSSGSSSTRDYYAMFSPRATLLESNSMTKLSSAQPFQGGFLLEKVMGPLSTGHLELKTR- 429

Query: 432 DVKKSPIVQFNYYSHPDDLARCVKGVRKLGDVFKTPTMEKIKTQDLKGNKGFMFLGLPLP 491
           + K +PIV FNY+ HPDDL RCV+G++ +  V ++    + K  D+   +  + L    P
Sbjct: 430 NPKDNPIVTFNYFQHPDDLKRCVRGIQTIERVVQSKAFSRYKYADV-SFEYLLNLTASTP 489

Query: 492 ENLWN---------DSAVEEYCKKTVATYWHYHGGSLVGKVVDGNYKVIGTENLRVVDGS 532
            NL             + EE+C+ TV T WHYHGG +VG+VVDG+YKVIG + LRV+D S
Sbjct: 490 VNLRPPRSGPGASLPPSAEEFCQHTVTTIWHYHGGCVVGRVVDGDYKVIGIDRLRVIDMS 549

BLAST of Chy5G099710 vs. TAIR 10
Match: AT5G51930.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 370.2 bits (949), Expect = 2.9e-102
Identity = 228/528 (43.18%), Postives = 306/528 (57.95%), Query Frame = 0

Query: 39  FVHNATDLPTKEEYDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNIYPSVLNEQGLS 98
           F+ +AT  P    +DYIIIGGGTAGC LA TLS   SVL+LERG  P   P+   + G S
Sbjct: 60  FMKDATLAPKNASFDYIIIGGGTAGCALAATLSQNASVLVLERGGSPYENPTA-TDMGNS 119

Query: 99  KAFDTEDDGKTPFQRFISEDGVENIRGRVLGGGSMVNAGFYSRGHKEFFETAGVDWDMEL 158
                 +   +  Q FISEDGV N R RVLGGGS++N GFYSR   ++ E A  +W+ME 
Sbjct: 120 VNTLLNNTPNSWSQLFISEDGVYNTRPRVLGGGSVINGGFYSRAGNDYVEEA--EWEMEE 179

Query: 159 VEKAYEWVEETVVSQPILKDWQSAFRTALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGN 218
           VE AYEWVE+ +V +P + +WQ AF+  LLE G  PDNGF   H+ GTK GG+I D  G+
Sbjct: 180 VEAAYEWVEKKLVFEPQVIEWQKAFKDGLLEAGESPDNGFTYDHIYGTKIGGTIFDRAGH 239

Query: 219 RHGAVELLNKANPKNLKVAIEATVQRILFSDLSANGVSYLDSTGKLHTAFIHEK--GEIF 278
           RH A  LL  ANP  + V + A+V ++LF+   A  V + D+ G  H A +  K   E+ 
Sbjct: 240 RHTAANLLEYANPNRIVVYLHASVHKVLFT-TEAYEVLFEDANGVFHKANLANKATNEVI 299

Query: 279 LSAGAIGSPQLLLLSGIGPKSYLSSLKL-PVVLHQPHVGQSMSDNPRFGTNIALPFQVVP 338
           LSAGA+GSPQLL+LSG+GP  +L +  + P+VL QP VGQ M+DNP     I  P  V  
Sbjct: 300 LSAGALGSPQLLMLSGVGPAVHLEAHGVNPLVLDQPMVGQGMADNPMNFVAIPSPQPVEL 359

Query: 339 TSGKVVGILQDNIYFQSLASPS-PFLVPPTF-----SLL--PPHPT-------SINPTLA 398
           +  + VGI + + Y + L+  S  F +   F     +LL    H T       SI   L 
Sbjct: 360 SLIQAVGITKFDSYIEGLSGLSLSFDITRRFFDGVLNLLNETSHTTSRKILTQSIAVLLK 419

Query: 399 TF-------------FGKFSEVHSKGSLKLNSSIDVKKSPIVQFNYYSHPDDLARCVKGV 458
           +F             F K     SKG +KL ++ + + +P V FNYY  P+DL +CVKG+
Sbjct: 420 SFDVKLEVRMNGGLIFQKVDGPASKGHMKLRNT-NPRDNPSVTFNYYQEPEDLNKCVKGL 479

Query: 459 RKLGDVFKTPTMEKIKTQDLKGNKGF-MFLGLPL---PENLWNDSAVEEYCKKTVATYWH 518
             +  +  +    K K   +   +   + L LP+   P ++ +   ++++C  TV + WH
Sbjct: 480 NTIIRMINSKAFSKYKYPGVTARELLNLMLALPINLRPRHVTSAFNLKQFCIDTVTSVWH 539

Query: 519 YHGGSLVGKVVDGNYKVIGTENLRVVDGSTFSVSPGTNPMATLMMLGR 532
           YHGG  VGKVVD NYKV+G + LRV+DGSTF  SPGTNP AT+MMLGR
Sbjct: 540 YHGGCQVGKVVDKNYKVLGIDGLRVIDGSTFLKSPGTNPQATVMMLGR 582

BLAST of Chy5G099710 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 367.9 bits (943), Expect = 1.4e-101
Identity = 222/522 (42.53%), Postives = 289/522 (55.36%), Query Frame = 0

Query: 52  YDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDP--NIYPSVLNEQGLSKAFDTEDDGKT 111
           YDYI+IGGGTAGCPLA TLS  FSVL+LERG  P  N   S L    +  A   +    +
Sbjct: 63  YDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRNFHIGLA---DISASS 122

Query: 112 PFQRFISEDGVENIRGRVLGGGSMVNAGFYSRGHKEFFETAGVDWDMELVEKAYEWVEET 171
             Q F+S DGV N R RVLGGGS +NAGFYSR    F + AG  WD +LV+++Y WVE  
Sbjct: 123 ASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRAG--WDPKLVKESYPWVERE 182

Query: 172 VVSQPILKDWQSAFRTALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKA 231
           +V QP L  WQ A R +LLE G+ P NGF   H+ GTK GG+I D  G RH A ELL  A
Sbjct: 183 IVHQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAELLAYA 242

Query: 232 NPKNLKVAIEATVQRILFSDLS----ANGVSYLDSTGKLHTAFIHEK--GEIFLSAGAIG 291
           NP+ L+V I ATVQ+I+F          GV + D  G  H A +  +   E+ LS+GAIG
Sbjct: 243 NPQKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSSGAIG 302

Query: 292 SPQLLLLSGIGPKSYLSSLKLPVVLHQPHVGQSMSDNPRFGTNIALPFQVVPTSGKVVGI 351
           SPQ+L+LSGIGPK  L  LK+PVVL   HVG+ M+DNP     +     +  +  + VGI
Sbjct: 303 SPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPMNTILVPSKAPIEQSLIQTVGI 362

Query: 352 LQDNIYFQSLA--SPSPFLV----------PPTFSLLPPHPTSINPTLATFFGKFSEVH- 411
            +  +Y ++      SP  +             FS +P        T A       ++H 
Sbjct: 363 TKMGVYVEASTGFGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITRNKYQLHE 422

Query: 412 ---------------SKGSLKL-NSSIDVKKSPIVQFNYYSHPDDLARCVKGVRKLGDVF 471
                          S+G L L N+++D   +P V FNY+ HP DL RCV+ +R +  V 
Sbjct: 423 AFNGSFILEKLAYPISRGHLSLVNTNVD--DNPSVTFNYFKHPVDLQRCVEAIRLVSKVV 482

Query: 472 KTPTMEKIKTQDLKGNKGFMFLGLPL-----PENLWNDSAVEEYCKKTVATYWHYHGGSL 531
            +         D +     + L +       P+ L +  ++ ++CK TV T WHYHGG L
Sbjct: 483 TSNRFLNYTQCDKQNVHKMLSLSVKANINLRPKQLNDTKSMAQFCKDTVVTIWHYHGGCL 542

BLAST of Chy5G099710 vs. TAIR 10
Match: AT3G56060.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 364.8 bits (935), Expect = 1.2e-100
Identity = 225/531 (42.37%), Postives = 301/531 (56.69%), Query Frame = 0

Query: 38  KFVHNATDLPTKEEYDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNIYPSVLNEQGL 97
           +F+ +AT  P    +DYIIIGGGTAGC LA TLS   +VL+LERG  P   P+  +    
Sbjct: 32  RFMKDATLAPKLSHFDYIIIGGGTAGCALAATLSQNATVLVLERGGSPYDDPAATDIGNF 91

Query: 98  SKAFDTEDDGKTPFQRFISEDGVENIRGRVLGGGSMVNAGFYSRGHKEFFETAGVDWDME 157
           +          +  Q FISEDGV N R RVLGGG+++NAGFYSR  ++F   AG  W+ +
Sbjct: 92  ANTL-LNITPNSWSQLFISEDGVFNSRARVLGGGTVINAGFYSRAEEDFVAEAG--WERD 151

Query: 158 LVEKAYEWVEETVVSQPILKDWQSAFRTALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKG 217
            VE AYEWVE+ VV +P +  WQSAFR  LLE G+ P NGF   H+VGTK GG+I D  G
Sbjct: 152 EVEAAYEWVEKKVVFEPPVNKWQSAFRDGLLEAGVTPYNGFTYEHIVGTKFGGTIFDRDG 211

Query: 218 NRHGAVELLNKANPKNLKVAIEATVQRILFS-----DLSANGVSYLDSTGKLHTAFIHEK 277
           +RH A  LL  ANP  + V + A+V +ILF+        A GV +LD+ G  + A +  +
Sbjct: 212 HRHTAANLLEYANPNMIVVYLHASVHKILFTIKGNQRPKAYGVIFLDANGVSYKAELATQ 271

Query: 278 ----GEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKL-PVVLHQPHVGQSMSDNPRFGTNI 337
                E+ LSAGAI SPQLL+LSG+GP ++L++ ++ PV++ QP VGQ M DNP     I
Sbjct: 272 DSTMSEVILSAGAIASPQLLMLSGVGPAAHLAAYRVNPVIVDQPMVGQGMGDNPMNPVFI 331

Query: 338 ALPFQVVPTSGKVVGILQDNIYFQ--SLASPSPFLVPPTF---------SLLPPHPTS-- 397
             P  V  +  + VGI +   Y +  S  S S  L    F         + LP    S  
Sbjct: 332 PSPEPVEVSLVQAVGITKFGSYIEGGSALSLSISLTRSFFDGVLNLLKKTKLPTQSISKF 391

Query: 398 ---------INPTLATFFGKFSEVHSKGSLKLNSSIDVKKSPIVQFNYYSHPDDLARCVK 457
                    +         K +   S+G L+L ++ +   +P V FNY+  P+DL +CV+
Sbjct: 392 FKSLDLTLNVTTKAGVIIQKVNGPLSRGHLELRNT-NPDDNPSVTFNYFKDPEDLNKCVE 451

Query: 458 GVRKLGDVFKTPTMEKIKTQDLKGNKGFMFLGLPLPENL-----WNDSAVEEYCKKTVAT 517
           G+  +  V  +    K K   L   +G + L L LP NL      +   +E+YC  TV T
Sbjct: 452 GLSTIIKVIDSKGYSKYK-YPLASARGLLNLILALPTNLRPRHITSTFDLEQYCIDTVMT 511

Query: 518 YWHYHGGSLVGKVVDGNYKVIGTENLRVVDGSTFSVSPGTNPMATLMMLGR 532
            +HYHGG  VGKVVD NYKV+G + LR++DGSTF  SPGTNP AT+MMLGR
Sbjct: 512 IYHYHGGCQVGKVVDNNYKVLGVDALRIIDGSTFLKSPGTNPQATIMMLGR 557

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P527063.1e-15451.32(R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1[more]
Q945K21.2e-15050.09(R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1[more]
P527074.9e-14749.81(R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1[more]
O827841.2e-14549.25(R)-mandelonitrile lyase 4 OS=Prunus serotina OX=23207 GN=MDL4 PE=2 SV=1[more]
O242431.5e-14348.50(R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LZU81.9e-29595.10Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G627390 PE=3 SV=1[more]
A0A1S3BSR31.8e-28291.53(R)-mandelonitrile lyase 1-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC1034931... [more]
A0A1S3BSS84.1e-26691.38(R)-mandelonitrile lyase 1-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC1034931... [more]
A0A5A7VHS63.0e-26491.52(R)-mandelonitrile lyase 1-like isoform X1 OS=Cucumis melo var. makuwa OX=119469... [more]
A0A1S3BTY83.0e-26491.52(R)-mandelonitrile lyase 1-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC1034931... [more]
Match NameE-valueIdentityDescription
XP_011650118.20.095.86(R)-mandelonitrile lyase 1-like [Cucumis sativus] >KGN61134.2 hypothetical prote... [more]
XP_031736583.10.094.73(R)-mandelonitrile lyase 1 [Cucumis sativus] >KAE8651658.1 hypothetical protein ... [more]
XP_008451990.10.091.53PREDICTED: (R)-mandelonitrile lyase 1-like isoform X1 [Cucumis melo][more]
XP_008452005.20.091.38PREDICTED: (R)-mandelonitrile lyase 1-like isoform X2 [Cucumis melo][more]
XP_008451997.10.091.52PREDICTED: (R)-mandelonitrile lyase 1-like isoform X3 [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT1G73050.12.6e-14348.23Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G12570.12.6e-11142.58Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT5G51930.12.9e-10243.18Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G72970.11.4e-10142.53Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT3G56060.11.2e-10042.37Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.410.40coord: 172..480
e-value: 4.9E-166
score: 555.4
NoneNo IPR availablePANTHERPTHR45968OSJNBA0019K04.7 PROTEINcoord: 5..531
NoneNo IPR availablePANTHERPTHR45968:SF23(R)-MANDELONITRILE LYASE 4-RELATEDcoord: 5..531
NoneNo IPR availableSUPERFAMILY54373FAD-linked reductases, C-terminal domaincoord: 376..487
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 52..530
e-value: 4.9E-166
score: 555.4
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 33..531
IPR012132Glucose-methanol-choline oxidoreductasePIRSFPIRSF000137Alcohol_oxidasecoord: 32..347
e-value: 5.4E-57
score: 191.8
coord: 345..531
e-value: 1.5E-28
score: 97.9
IPR007867Glucose-methanol-choline oxidoreductase, C-terminalPFAMPF05199GMC_oxred_Ccoord: 390..530
e-value: 2.2E-26
score: 93.1
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPFAMPF00732GMC_oxred_Ncoord: 52..321
e-value: 1.5E-30
score: 106.6
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00623GMC_OXRED_1coord: 125..148
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00624GMC_OXRED_2coord: 280..294

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy5G099710.1Chy5G099710.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016829 lyase activity
molecular_function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors