Chy5G099700 (gene) Cucumber (hystrix) v1

Overview
NameChy5G099700
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Description(R)-mandelonitrile lyase 1-like
LocationchrH05: 13863842 .. 13869625 (+)
RNA-Seq ExpressionChy5G099700
SyntenyChy5G099700
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTAGAAATAAAATTAGAAAAGAGAAACAAGCAAAGATATGCTAAACGTTTTTGCATACATTGGCTGCCTCTTCCAAAATTATCAATACAAAAAGAAATATAAGAAAAAAAGAAATAGAGTTGCGATACCCAATATTTAGGCGAGGACAATGGTAGCACTATTAATTAGGACCAATAATTTGGTGTGTTTTCCTTTTTTTTTTGTACATGTTTTGTTTTGTTCTTTTATGATCTTTTTGTGGTATCTGTCCAAGGCCACTATTGTGGCTCTCTCCTCCTTTCAATTCACATACCTACCTTCACTTCTTTCATGAGACCTTGCACCGGGGGACATAGTTTTCATCATTTACAAAGGGTATTAACTTGGCCTACCTTTAAATTTCTGCGTACAAAGCCCAACAAATATTAACCGGCCATTTATTTCTTATTAGACTTCTACAAATCTTAGACTCTATCACTTCTACTAGAGATATAGTCTCACCCTTACCACTAACAATAGATTCAAAGAGCTGGATTTAAAATTTTACTATAACTAGAAATCATTTTATATTGTGTTACATCTACTAATCGGATTGATATATTTGCAATTTATCTTTATATAAACTATGTCCTAAAGAAACTTTCTAAGTTAGAATTTTTTTTTTTTAAAAAAAAAAACTATAGAATTGACAGCAAATTTTCTCGTGAATAGCTTCGAAAGGGAATTTCGGTAAGGAGACCATCTTTTGATGTCTTGGAACTATGTGCCACATGTTTTGTGGAGCGGTAAATTTCCACGAGATATGTTTTTCTGTTTTCGGAAAACTTAAACATTGTACTAGCCGGAGGAAAAAAGAAAGGAAAAATTGATTCTCCCTGACCACCACAACACATTGGATAATCCAGCTTTAACATCAGTTCGTCCAGGCCTTCACTTAGTTTAATCCCAGTATTATCTTGGTCATCGATAGATTGAGAATTGAAGGAGAAACTGAGGAAGAGAAAGATCAAAACACGAAAAAAGAGGGATTGGAATTGTGGTTCGAGGATTTGGTGATATGAGTAGAGTTAATTTGATTTCTTGAAAATTAGAGAGATGATTAATGAAAGGTTTGGATGCTTAGTTATATTCCATAGATTCCATAGTTATTAGTACACTCACTTATTAATTGAATTAAGCTTTTATTTGTGATGCTAAATTTTTTAGAACATTGTTAATGTAATGCCTTAAATTTTACTGATATTTGGAGGGAAAATTTTTAAAAATAATAGATTTTACAAAATATTTACAATAGAATTTAGTTATTAATATGCAAGTAATAGAATCCAAAATTTTGTTATAGTTTTATTTTAGAAAACTATAAGTAATAGAATCCAAAATTTTGTTATAGTTTTATTTTAGAAAACTATAAGTAATAGAATCCAAAATTTTGTTATAGTTTTATTTTAGAAAACTATAAGTAATAGAATCCAAAATTTTGTTATAGTTTTATATTATTTTGTTATTTTAAAAAATGCTCCTATTATTTGGAGTGTAGGAAGAAAAAAAAAACAATAAAATAAATATCATAAATATCATAAATATCATAAATATGGGAGAAAAAGGGTGATAAGATAATCTAACCTCTTAGGTGGGTGGAGATAATAAATTAGGAGAGAGAAATTTGATTAAAAAGAAGTGAAATTGAATAACATAATGGTGTACAATGGTGAATAAAGAGAAAAACTTCATGAGAAAGGCAATATGGGAGAAAAGAAAGGTGTGGACTTCACAAGAAGTTTTGTTAGAAATTTTGAAAAAGTTATATGTATCTAAAATTAGGTTTAATTAAAATACCATTTTGATCATGAGCTTTGATATAAGGTGTTGATCTATTTTGACTCACTACATTCAATCTCCAATTTTAATACATACTATAATACTACTTTGAATGTAATTGCTACATTATTGCTATTGTTTAAAAGAGAGAATTTGTTTAATTAATTCATATTTTTATCCCTATTTTTCTACTAGCCTTTTGTTGCTTTATTTTGATCAAATACACAGAGTCAGTTGATGATAATATTCCATCAAGGTCATTGGTAAATGTGACTTTTAGCTTAGCGTTGATCAAGATTGTTTCAAGAGAAAAGATTTTTAGTTGAATTCTTTGGATAAAATGATTTAAATTAAAATACACATCAAAAGTTATTTAGCTAGTTATATATAGTAGTTGATACAAGAAAAAAAATAATTAAAAAAGTAAACTCAAAGATCAACCGTGTAAAGAAAAACAAATTTTCATTTTAAAGATATTGATAGACAAAATGGCAAAAGCTCTCTTAATTTGATTGTGCTAATTAATTAATTTTTTTCTTAAGAATGTGTTAAAAATATTAATTTAAATATATACAAAAAAAACAAAAAACTGAATAGATTTGACTAACTATAATCATCATAGGTTGATTTGTGGTAAATAATTTTTTTAAAAAAAAATATAATGGATCAGTTAGATTTTTATGATAAATAATTAAAATGTAAATAAAGGCTTTGATAAGATGGCATAAATTCAATAGTTGTCCCGGCCTGTCTAAGATATAATGTTCTAAGAGTTTTTACTGATACCTAAATGTTTTAGGATTGGAGGTACTGTCTTTCAAAATCAGTAAATTGGACACACTCCGGTATAAAAACAAAAATTAACCAAACCATAGCTTGCAATTAAATTAAGTGTCCAACTTTGTAAACCTAAATATATATATATATACCAACAGTTTGCAAATTAGAAAGGAACAAAGAAGTACCCCCACATATACATCTACATCTTTGAATAAGCATGGAGTATTCAATGGCAACCTTTCTCCCTCTCATCCTAATCTCCATTGAATTTCTCTCTTCACATGCCATCCCTAATCAAGGCAAAACCCCTGATCTCTCTATATCTTTCTTTTTCTAAACTTATATCATCTTTAATTTATTGATTCTAACTGTTGTGTATTTTGTTACCTTTCCTTTTCTAGATATTAGCTACATGAAATTTGTACACAATGCCTATGATCAACCAATACAAGAAAAATATGACTACATAGTAATAGGAGGAGGAACAACAGGCTGTCCATTAGCTACAACATTATCATCAAAATTCTCAGTCCTCCTTCTTGAAAGAGGTAGTGACCCCAACACATATCCCTTTGTCTTGAATGAAGAAACTCTATCCTACGTTTTTATTATAGATGATGATGGCCAAAATCCCATCCAACGTTTCGTCTCTGAGGATGGTGTCGAGAACATCCGAGGGCGAGTTCTTGGCGGCGGAAGCATGGTCAATGCCGGGTTCTACTCAAGGGGACATCAAGAGTTTTTTGAAACTGCAGGTGTGGATTGGGACATGGAATTGGTTGAGAAGGCTTACGAATGGGTTGAAGAAACTTTGGTGTCTAGGCCAAATTTAACTTCTTGGCAAGCTGCTTTTAGAAATACTTTGTTGGAAGGTGGTGTTGTCCCTGATAATGGGTTTGATTTGAGGCATCTTGTGGGTACTAAAATTGGAGGTTCAATCTTTGATAACAAAGGAAATAGACATGGAGCTGTAGAGCTTCTCAATAAGGCTAATCCTAAAAATCTTAAGGTTGTCATTCAAGCCACAGTTCAAAGAATCATCTTCAAAGGTTAGTGGCTAACAAGTGAAATTTGGTCTTTTTTTTTTGGTTAATGAATTTTTTGAAGATGGTTTCTAACTTTTAAAAAGATAAGGGATTTTTTTTTTATATTGATATTAGAGAAAAAAATAATGATATTTTGGTCCATTTTAAGATGCATTTTCATCACCCAACTCACTCTCACTCATCACGAAAGTAAATATAATTTTTTTAAAAATTAATCTTTTTAGTACTCAGATATAATTTTGAAGAATAAGGTGGGTTGGATTCTTAAGTTTTTAAAACACACCTTATCCTGTTATTATATAAAACTAAAATAGAAAACAGTTCCATGCACCTTTTGAACTTTTTTTTTTTTATAATTTCAAATTTAAGGACATATATTTTTAAAACTCAAGGAAAAATACTCGTTTTTTTTTTTTTAAAAAAATACGGTTTTGGGTCAGATCTGTTTTAAAGACTCAAAATTTTTTTTTAAAGAATTAAAAGATCAAAACTCACTGGTTCATAGACTAATACAGTAGTTTGCAGAATAAAAAATAAATATTTTTTTGAGAAAATGGTGATGATCCAGTCTATGTGTCTCCAAAACATAATCACATCAATGTCACGTTAATAAACGCATTACAACGATATACCAATGGTTAAAACTTAAAATCAAAACGAAATTAAACTTATCATCTCTACCTAAATAAATAATAATAATAATAAAAACTTATGTTAACTACTACAAAATCTACAACTTACAATTTTTAACAGTTTTATCGAAAAAAATGTCGAGTAATGTATTTAGAAACGTAAAATAGATAACATAATAAGCAGAAATTTCACAGAAGTTTTCGATCTTTTTTTTTTCACGTTTCTCTACTTCCAACTAATTACAGGTTTATCAGCAGTTGGAGTTTTATATTCAGATTCAAAAGGAAAGTTACACACGGCATTAATTCATAAAAAAGGAGAAATATTTTTAAGTGCAGGAGCTATTGGAAGTCCTCAACTTCTCCTTTTAAGTGGGATTGGCCCAAAATCTTATCTTTCATCTTTAAAACTACATGTCGTTCACCACCAACCACATGTGGGCCAATACATGACCGACAATCCCCGTTTTAGTCGTTCTATTATTTTTCCATTCCAGTTACTTGCTTCAACAGCGCAAGTGGTTGGAACATTAGAAAAAAACATCCATTTACAATCATTGGCCAGCCCTTTACCATTTTTCCCTTTACCATCTTATGGTCTTCTTCCTCCTCAATCCACTTCCATCACTTCAAGCTTAGTTATTTTTGTAGGTAAATTTGCTAATGTTAGTTCTAAAGGGTGGCTTCATTTGAATAATTCTTCCACTGATGCGAAGGAGAATCCTATTGTTAGATTCAATTATTATTCACAGCATGGTGATCTTAGTAGATGTGTGAGTGGAGTGAGGAAAATGGAGGATTTGCTTAAGACACAAACTATGGAAAGGATTAAGACACAAGATTTGGAGGGTAATAATGGATTTCAATTTATGGGACTTCCAATGCCTGAAAATTTGTGGAATGATAGCTCTGTTGAAGAGTATTGCAAAAATACGGTGGCTACTTATTGGCATTATCATGGAGGATGTTTGGTTGGAAAAGTTGTGGATGATAATTATAAAGTTATTGGAATTAAAAATTTGCGTGTTGTTGATGGCTCCACTTTCTCCGACTCGCCAGGAACTAATCCTATGGCCACCCTTATGATGTTAGGCCGGTGAGTTTATTTTTATTTTTATTTTTTACTTGGTGGATCTCTCAAAATATATGTGTGTGTGTTTGGTTTGGTTTTTGGTTTAATATATAATATAAACAGTTTGTGTTTTGAACCTTGATACCTGTTTATAAGAAGCATTTTTCTAGTAAAAAAAGATGCTACTTATTGTTATTATTTGAAATAAATGAAGGGCTTGGAAAACTAATTTTTAAATGATTTATAAGATCAGCATAATGCAATTTATGCATTATTAGTTTTTTTCAAAAAGATAAAAAGAAGGAACCCTCCACTATTACCTGAACCTTTGCATTATTAGTTGAACTTAGTTTTTTCTCTCTTTTGCATTATCTTTTATTTCCATAAACTTACAAGTTCTAATTTGTTTTGCAGATATGTTGGCCTTAAAGTAGTGCAACAAAGATCAAGTTAA

mRNA sequence

ATGCTAGAAATAAAATTAGAAAAGAGAAACAAGCAAAGATATGCTAAACATATTAGCTACATGAAATTTGTACACAATGCCTATGATCAACCAATACAAGAAAAATATGACTACATAGTAATAGGAGGAGGAACAACAGGCTGTCCATTAGCTACAACATTATCATCAAAATTCTCAGTCCTCCTTCTTGAAAGAGGTAGTGACCCCAACACATATCCCTTTGTCTTGAATGAAGAAACTCTATCCTACGTTTTTATTATAGATGATGATGGCCAAAATCCCATCCAACGTTTCGTCTCTGAGGATGGTGTCGAGAACATCCGAGGGCGAGTTCTTGGCGGCGGAAGCATGGTCAATGCCGGGTTCTACTCAAGGGGACATCAAGAGTTTTTTGAAACTGCAGGTGTGGATTGGGACATGGAATTGGTTGAGAAGGCTTACGAATGGGTTGAAGAAACTTTGGTGTCTAGGCCAAATTTAACTTCTTGGCAAGCTGCTTTTAGAAATACTTTGTTGGAAGGTGGTGTTGTCCCTGATAATGGGTTTGATTTGAGGCATCTTGTGGGTACTAAAATTGGAGGTTCAATCTTTGATAACAAAGGAAATAGACATGGAGCTGTAGAGCTTCTCAATAAGGCTAATCCTAAAAATCTTAAGGTTGTCATTCAAGCCACAGTTCAAAGAATCATCTTCAAAGGTTTATCAGCAGTTGGAGTTTTATATTCAGATTCAAAAGGAAAGTTACACACGGCATTAATTCATAAAAAAGGAGAAATATTTTTAAGTGCAGGAGCTATTGGAAGTCCTCAACTTCTCCTTTTAAGTGGGATTGGCCCAAAATCTTATCTTTCATCTTTAAAACTACATGTCGTTCACCACCAACCACATGTGGGCCAATACATGACCGACAATCCCCGTTTTAGTCGTTCTATTATTTTTCCATTCCAGTTACTTGCTTCAACAGCGCAAGTGGTTGGAACATTAGAAAAAAACATCCATTTACAATCATTGGCCAGCCCTTTACCATTTTTCCCTTTACCATCTTATGGTCTTCTTCCTCCTCAATCCACTTCCATCACTTCAAGCTTAGTTATTTTTGTAGGTAAATTTGCTAATGTTAGTTCTAAAGGGTGGCTTCATTTGAATAATTCTTCCACTGATGCGAAGGAGAATCCTATTGTTAGATTCAATTATTATTCACAGCATGGTGATCTTAGTAGATGTGTGAGTGGAGTGAGGAAAATGGAGGATTTGCTTAAGACACAAACTATGGAAAGGATTAAGACACAAGATTTGGAGGGTAATAATGGATTTCAATTTATGGGACTTCCAATGCCTGAAAATTTGTGGAATGATAGCTCTGTTGAAGAGTATTGCAAAAATACGGTGGCTACTTATTGGCATTATCATGGAGGATGTTTGGTTGGAAAAGTTGTGGATGATAATTATAAAGTTATTGGAATTAAAAATTTGCGTGTTGTTGATGGCTCCACTTTCTCCGACTCGCCAGGAACTAATCCTATGGCCACCCTTATGATGTTAGGCCGATATGTTGGCCTTAAAGTAGTGCAACAAAGATCAAGTTAA

Coding sequence (CDS)

ATGCTAGAAATAAAATTAGAAAAGAGAAACAAGCAAAGATATGCTAAACATATTAGCTACATGAAATTTGTACACAATGCCTATGATCAACCAATACAAGAAAAATATGACTACATAGTAATAGGAGGAGGAACAACAGGCTGTCCATTAGCTACAACATTATCATCAAAATTCTCAGTCCTCCTTCTTGAAAGAGGTAGTGACCCCAACACATATCCCTTTGTCTTGAATGAAGAAACTCTATCCTACGTTTTTATTATAGATGATGATGGCCAAAATCCCATCCAACGTTTCGTCTCTGAGGATGGTGTCGAGAACATCCGAGGGCGAGTTCTTGGCGGCGGAAGCATGGTCAATGCCGGGTTCTACTCAAGGGGACATCAAGAGTTTTTTGAAACTGCAGGTGTGGATTGGGACATGGAATTGGTTGAGAAGGCTTACGAATGGGTTGAAGAAACTTTGGTGTCTAGGCCAAATTTAACTTCTTGGCAAGCTGCTTTTAGAAATACTTTGTTGGAAGGTGGTGTTGTCCCTGATAATGGGTTTGATTTGAGGCATCTTGTGGGTACTAAAATTGGAGGTTCAATCTTTGATAACAAAGGAAATAGACATGGAGCTGTAGAGCTTCTCAATAAGGCTAATCCTAAAAATCTTAAGGTTGTCATTCAAGCCACAGTTCAAAGAATCATCTTCAAAGGTTTATCAGCAGTTGGAGTTTTATATTCAGATTCAAAAGGAAAGTTACACACGGCATTAATTCATAAAAAAGGAGAAATATTTTTAAGTGCAGGAGCTATTGGAAGTCCTCAACTTCTCCTTTTAAGTGGGATTGGCCCAAAATCTTATCTTTCATCTTTAAAACTACATGTCGTTCACCACCAACCACATGTGGGCCAATACATGACCGACAATCCCCGTTTTAGTCGTTCTATTATTTTTCCATTCCAGTTACTTGCTTCAACAGCGCAAGTGGTTGGAACATTAGAAAAAAACATCCATTTACAATCATTGGCCAGCCCTTTACCATTTTTCCCTTTACCATCTTATGGTCTTCTTCCTCCTCAATCCACTTCCATCACTTCAAGCTTAGTTATTTTTGTAGGTAAATTTGCTAATGTTAGTTCTAAAGGGTGGCTTCATTTGAATAATTCTTCCACTGATGCGAAGGAGAATCCTATTGTTAGATTCAATTATTATTCACAGCATGGTGATCTTAGTAGATGTGTGAGTGGAGTGAGGAAAATGGAGGATTTGCTTAAGACACAAACTATGGAAAGGATTAAGACACAAGATTTGGAGGGTAATAATGGATTTCAATTTATGGGACTTCCAATGCCTGAAAATTTGTGGAATGATAGCTCTGTTGAAGAGTATTGCAAAAATACGGTGGCTACTTATTGGCATTATCATGGAGGATGTTTGGTTGGAAAAGTTGTGGATGATAATTATAAAGTTATTGGAATTAAAAATTTGCGTGTTGTTGATGGCTCCACTTTCTCCGACTCGCCAGGAACTAATCCTATGGCCACCCTTATGATGTTAGGCCGATATGTTGGCCTTAAAGTAGTGCAACAAAGATCAAGTTAA

Protein sequence

MLEIKLEKRNKQRYAKHISYMKFVHNAYDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLNEETLSYVFIIDDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTSWQAAFRNTLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRIIFKGLSAVGVLYSDSKGKLHTALIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLHVVHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFPLPSYGLLPPQSTSITSSLVIFVGKFANVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDLSRCVSGVRKMEDLLKTQTMERIKTQDLEGNNGFQFMGLPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVVQQRSS*
Homology
BLAST of Chy5G099700 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 8.8e-149
Identity = 257/520 (49.42%), Postives = 357/520 (68.65%), Query Frame = 0

Query: 19  SYMKFVHNAYDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLNE 78
           SY+ FV++A D  ++  YDYI++GGGT GCPLA TLS+ +SVL+LERGS P  YP +L  
Sbjct: 37  SYLSFVYDATDPELEGSYDYIIVGGGTAGCPLAATLSANYSVLVLERGSLPTEYPNLLIS 96

Query: 79  ETLSYVFIIDDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHQEFFETAGVDW 138
           +   Y    +DDG+ P++RFVSEDG++N+RGRVLGG SM+NAG Y R +  FF   G++W
Sbjct: 97  DGFVYNLQQEDDGKTPVERFVSEDGIDNVRGRVLGGTSMINAGVYVRANTSFFNQTGIEW 156

Query: 139 DMELVEKAYEWVEETLVSRPNLTSWQAAFRNTLLEGGVVPDNGFDLRHLVGTKIGGSIFD 198
           DM+LV + YEWVE+T+V  P+  +WQ       LE G++P+NGF + HL GT++ GS FD
Sbjct: 157 DMDLVNQTYEWVEDTIVFEPDSQTWQTVIGTAYLEAGILPNNGFSVDHLAGTRLTGSTFD 216

Query: 199 NKGNRHGAVELLNKANPKNLKVVIQATVQRIIF----KGLSAVGVLYSDSKGKLHTALIH 258
           N G RH + ELLNK +P NL+V +QA V++IIF     G++A+GV+Y+DS G  H A + 
Sbjct: 217 NNGTRHASDELLNKGDPNNLRVAVQAAVEKIIFSSNTSGVTAIGVIYTDSNGTTHQAFVR 276

Query: 259 KKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLHVVHHQPHVGQYMTDNPRFSRSIIFP 318
            +GE+ LSAG IGSPQLLLLSG+GP+SYL+SL + VV   P+VGQY+ DNPR   +I+ P
Sbjct: 277 GEGEVILSAGPIGSPQLLLLSGVGPESYLTSLNISVVASHPYVGQYIYDNPRNFINILPP 336

Query: 319 FQLLASTAQVVGTLEKNIHLQSLAS------PLPFFPLPSYGLLPPQSTSITSSLVIFVG 378
             + AST  V+G +  + +  S++S      P  FFP  SY L  P  T         V 
Sbjct: 337 NPIEASTVTVLG-ITSDFYQCSISSLPFDTPPFSFFPTTSYPL--PNQT-----FAHIVN 396

Query: 379 KFANVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDLSRCVSGVRKMEDLLKTQTMERIK 438
           K     S G + L NSS+D +  P V+FNYYS   DLS CVSG++K+ ++L T  +E  K
Sbjct: 397 KVPGPLSHGTVTL-NSSSDVRVGPNVKFNYYSNLTDLSHCVSGMKKLGEVLSTDALEPYK 456

Query: 439 TQDLEGNNGFQFMGLPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGI 498
            +DL G +GF  +G+P+PEN  +D++ E +C+ +VA+YWHYHGGCLVGKV+DD ++V GI
Sbjct: 457 VEDLPGIDGFNILGIPLPENQTDDAAFETFCRESVASYWHYHGGCLVGKVLDDGFRVTGI 516

Query: 499 KNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVVQQRSS 529
             LRVVDGSTF  +P ++P    +MLGRY+G++++Q+RS+
Sbjct: 517 NALRVVDGSTFPSTPASHPQGFYLMLGRYMGIQILQERSA 547

BLAST of Chy5G099700 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 517.7 bits (1332), Expect = 1.6e-145
Identity = 255/514 (49.61%), Postives = 352/514 (68.48%), Query Frame = 0

Query: 19  SYMKFVHNAYDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLNE 78
           SY+ F ++A D  ++  YDY+++GGGT+GCPLA TLS K+ VL+LERGS P  YP VL  
Sbjct: 37  SYLSFAYDATDLELEGSYDYVIVGGGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVLTA 96

Query: 79  ETLSYVFIIDDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHQEFFETAGVDW 138
           +   Y    +DDG+ P++RFVSEDG++N+RGRVLGG S++NAG Y+R +   +  +GVDW
Sbjct: 97  DGFVYNLQQEDDGKTPVERFVSEDGIDNVRGRVLGGTSIINAGVYARANTSIYSASGVDW 156

Query: 139 DMELVEKAYEWVEETLVSRPNLTSWQAAFRNTLLEGGVVPDNGFDLRHLVGTKIGGSIFD 198
           DM+LV + YEWVE+T+V +PN  SWQ+  +   LE GV P++GF L H  GT+I GS FD
Sbjct: 157 DMDLVNQTYEWVEDTIVYKPNSQSWQSVTKTAFLEAGVHPNHGFSLDHEEGTRITGSTFD 216

Query: 199 NKGNRHGAVELLNKANPKNLKVVIQATVQRIIFK---GLSAVGVLYSDSKGKLHTALIHK 258
           NKG RH A ELLNK N  NL+V + A+V++IIF    GL+A GV+Y DS G  H A +  
Sbjct: 217 NKGTRHAADELLNKGNSNNLRVGVHASVEKIIFSNAPGLTATGVIYRDSNGTPHQAFVRS 276

Query: 259 KGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLHVVHHQPHVGQYMTDNPRFSRSIIFPF 318
           KGE+ +SAG IG+PQLLLLSG+GP+SYLSSL + VV   P+VGQ++ DNPR   +I+ P 
Sbjct: 277 KGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNIPVVLSHPYVGQFLHDNPRNFINILPPN 336

Query: 319 QLLASTAQVVGTLEKNIHLQSLASPLPFFPLPSYGLLPPQSTSI-TSSLVIFVGKFANVS 378
            +  +   V+G    N   Q   S LP F  P +G  P  S  +  S+   F  K A   
Sbjct: 337 PIEPTIVTVLGI--SNDFYQCSFSSLP-FTTPPFGFFPSASYPLPNSTFAHFASKVAGPL 396

Query: 379 SKGWLHLNNSSTDAKENPIVRFNYYSQHGDLSRCVSGVRKMEDLLKTQTMERIKTQDLEG 438
           S G L L  SS++ + +P V+FNYYS   DLS CVSG++K+ +LL T  ++  K +DL G
Sbjct: 397 SYGSLTL-KSSSNVRVSPNVKFNYYSNLTDLSHCVSGMKKIGELLSTDALKPYKVEDLPG 456

Query: 439 NNGFQFMGLPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVV 498
             GF  +G+P+P++  +D++ E +C+ +VA+YWHYHGGCLVGKV+D +++V GI  LRVV
Sbjct: 457 VEGFNILGIPLPKDQTDDAAFETFCRESVASYWHYHGGCLVGKVLDGDFRVTGINALRVV 516

Query: 499 DGSTFSDSPGTNPMATLMMLGRYVGLKVVQQRSS 529
           DGSTF  +P ++P    +MLGRYVG+K++Q+RS+
Sbjct: 517 DGSTFPYTPASHPQGFYLMLGRYVGIKILQERSA 546

BLAST of Chy5G099700 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 2.6e-145
Identity = 255/514 (49.61%), Postives = 350/514 (68.09%), Query Frame = 0

Query: 19  SYMKFVHNAYDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLNE 78
           SY++F ++A D  ++  YDY+++GGGT+GCPLA TLS K+ VL+LERGS P  YP VL  
Sbjct: 37  SYLRFAYDATDLELEGSYDYVIVGGGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVLTA 96

Query: 79  ETLSYVFIIDDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHQEFFETAGVDW 138
           +   Y    +DDG+ P++RFVSEDG++N+RGRVLGG SM+NAG Y+R +   +  +GVDW
Sbjct: 97  DGFVYNLQQEDDGKTPVERFVSEDGIDNVRGRVLGGTSMINAGVYARANTSIYSASGVDW 156

Query: 139 DMELVEKAYEWVEETLVSRPNLTSWQAAFRNTLLEGGVVPDNGFDLRHLVGTKIGGSIFD 198
           DM+LV K YEWVE+T+V +PN   WQ+      LE GV P++GF L H  GT+I GS FD
Sbjct: 157 DMDLVNKTYEWVEDTIVFKPNYQPWQSVTGTAFLEAGVDPNHGFSLDHEAGTRITGSTFD 216

Query: 199 NKGNRHGAVELLNKANPKNLKVVIQATVQRIIFK---GLSAVGVLYSDSKGKLHTALIHK 258
           NKG RH A ELLNK N  NL+V + A+V++IIF    GL+A GV+Y DS G  H A +  
Sbjct: 217 NKGTRHAADELLNKGNSNNLRVGVHASVEKIIFSNAPGLTATGVIYRDSNGTPHRAFVRS 276

Query: 259 KGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLHVVHHQPHVGQYMTDNPRFSRSIIFPF 318
           KGE+ +SAG IG+PQLLLLSG+GP+SYLSSL + VV   P+VGQ++ DNPR   +I+ P 
Sbjct: 277 KGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNIPVVLSHPYVGQFLHDNPRNFINILPPN 336

Query: 319 QLLASTAQVVGTLEKNIHLQSLASPLPFFPLPSYGLLPPQSTSI-TSSLVIFVGKFANVS 378
            +  +   V+G    N   Q   S LP F  P +   P  S  +  S+   F  K A   
Sbjct: 337 PIEPTIVTVLGI--SNDFYQCSFSSLP-FTTPPFSFFPSTSYPLPNSTFAHFASKVAGPL 396

Query: 379 SKGWLHLNNSSTDAKENPIVRFNYYSQHGDLSRCVSGVRKMEDLLKTQTMERIKTQDLEG 438
           S G L L  SS++ + +P V+FNYYS   DLS CVSG++K+ +LL T  ++  K +DL G
Sbjct: 397 SYGSLTL-KSSSNVRVSPNVKFNYYSNPTDLSHCVSGMKKIGELLSTDALKPYKVEDLPG 456

Query: 439 NNGFQFMGLPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVV 498
             GF  +G+P+P++  +D++ E +C+ +VA+YWHYHGGCLVGKV+D +++V GI  LRVV
Sbjct: 457 IEGFNILGIPLPKDQTDDAAFETFCRESVASYWHYHGGCLVGKVLDGDFRVTGIDALRVV 516

Query: 499 DGSTFSDSPGTNPMATLMMLGRYVGLKVVQQRSS 529
           DGSTF  +P ++P    +MLGRYVG+K++Q+RS+
Sbjct: 517 DGSTFPYTPASHPQGFYLMLGRYVGIKILQERSA 546

BLAST of Chy5G099700 vs. ExPASy Swiss-Prot
Match: O24243 ((R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 1.3e-144
Identity = 253/518 (48.84%), Postives = 353/518 (68.15%), Query Frame = 0

Query: 15  AKHISYMKFVHNAYDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPF 74
           A   SY+KFV+NA D  ++  YDYIVIGGGT+GCPLA TLS K+ VLLLERG+    YP 
Sbjct: 33  AHDFSYLKFVYNATDTSLEGSYDYIVIGGGTSGCPLAATLSEKYKVLLLERGTIATEYPN 92

Query: 75  VLNEETLSYVFIIDDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHQEFFETA 134
            L  +  +Y     DDG+ P++RFVSEDG++N+R R+LGG +++NAG Y+R +  F+   
Sbjct: 93  TLTADGFAYNLQQQDDGKTPVERFVSEDGIDNVRARILGGTTIINAGVYARANISFYSQT 152

Query: 135 GVDWDMELVEKAYEWVEETLVSRPNLTSWQAAFRNTLLEGGVVPDNGFDLRHLVGTKIGG 194
           G++WD++LV K YEWVE+ +V +PN  SWQ+      LE G++PDNGF L H  GT++ G
Sbjct: 153 GIEWDLDLVNKTYEWVEDAIVVKPNNQSWQSVIGEGFLEAGILPDNGFSLDHEAGTRLTG 212

Query: 195 SIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRIIF----KGLSAVGVLYSDSKGKLHT 254
           S FDN G RH A ELLNK +P NL V +QA+V++I+F      LSA+GV+Y+DS G  H 
Sbjct: 213 STFDNNGTRHAADELLNKGDPNNLLVAVQASVEKILFSSNTSNLSAIGVIYTDSDGNSHQ 272

Query: 255 ALIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLHVVHHQPHVGQYMTDNPRFSRS 314
           A +   GE+ +SAG IG+PQLLLLSG+GP+SYLSSL + VV   P+VGQ++ +NPR   +
Sbjct: 273 AFVRGNGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNITVVQPNPYVGQFLYNNPRNFIN 332

Query: 315 IIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFPLPSYGLLPPQSTSI-TSSLVIFVGK 374
              P  + AS   V+G   ++ + Q   S LP F  P + L P  S  +  S+    V +
Sbjct: 333 NFPPNPIEASVVTVLGI--RSDYYQVSLSSLP-FSTPPFSLFPTTSYPLPNSTFAHIVSQ 392

Query: 375 FANVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDLSRCVSGVRKMEDLLKTQTMERIKT 434
                S G + L NSS+D +  P ++FNYYS   DL+ CVSG++K+ DLL+T+ +E  K 
Sbjct: 393 VPGPLSHGSVTL-NSSSDVRIAPNIKFNYYSNSTDLANCVSGMKKLGDLLRTKALEPYKA 452

Query: 435 QDLEGNNGFQFMGLPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIK 494
           +D+ G +GF ++G+P+PEN  +D+S E +C + VA+YWHYHGG LVGKV+DD+++V+GIK
Sbjct: 453 RDVLGIDGFNYLGVPLPENQTDDASFETFCLDNVASYWHYHGGSLVGKVLDDSFRVMGIK 512

Query: 495 NLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVVQQRS 528
            LRVVD STF   P ++P    +MLGRYVGL+++Q+RS
Sbjct: 513 ALRVVDASTFPYEPNSHPQGFYLMLGRYVGLQILQERS 546

BLAST of Chy5G099700 vs. ExPASy Swiss-Prot
Match: O50048 ((R)-mandelonitrile lyase 2 OS=Prunus serotina OX=23207 GN=MDL2 PE=2 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 9.4e-143
Identity = 251/522 (48.08%), Postives = 350/522 (67.05%), Query Frame = 0

Query: 15  AKHISYMKFVHNAYDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPF 74
           A   SY++FV++A D  ++  YDYI++GGGT GCPLA TLS+ +SVL+LERG+ P  YP 
Sbjct: 34  AHDFSYLEFVYDANDTELEGTYDYIIVGGGTAGCPLAATLSANYSVLVLERGTLPTEYPN 93

Query: 75  VLNEETLSYVFIIDDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHQEFFETA 134
           +L  +   Y    +DDGQ P++RFVS DG++N+RGRVLGG SM+NAG Y R +  FF   
Sbjct: 94  LLTSDGFIYNLQQEDDGQTPVERFVSGDGIDNVRGRVLGGTSMINAGVYVRANTSFFNQT 153

Query: 135 GVDWDMELVEKAYEWVEETLVSRPNLTSWQAAFRNTLLEGGVVPDNGFDLRHLVGTKIGG 194
           G++WDM+LV K Y+WVE+T+V +P+   WQ       LE G++PDNGF L HL GT++ G
Sbjct: 154 GIEWDMDLVNKTYDWVEDTIVFKPDFQFWQNLTGTAFLEVGILPDNGFSLDHLEGTRLTG 213

Query: 195 SIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRIIF----KGLSAVGVLYSDSKGKLHT 254
           S FDN G RH + ELLNK +P NL+V + A V++IIF     G++A+GV+Y+DS G  H 
Sbjct: 214 STFDNNGTRHASDELLNKGDPNNLRVAVHAAVEKIIFSSDSSGVTAIGVIYTDSNGTTHQ 273

Query: 255 ALIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLHVVHHQPHVGQYMTDNPRFSRS 314
           A +   GE+ LSAG IGSPQLLLLSG+G +SYL+SL + VV   P+VGQY+ DNPR   +
Sbjct: 274 AFVRGDGEVILSAGPIGSPQLLLLSGVGLESYLTSLNISVVASHPYVGQYIYDNPRNFIN 333

Query: 315 IIFPFQLLASTAQVVGTLEKNIHLQSLAS------PLPFFPLPSYGLLPPQSTSITSSLV 374
           I+ P  + AST  V+G +  + +  S++S      P  FFP P+Y L  P +T       
Sbjct: 334 ILPPNPIEASTVTVLG-ITSDFYQCSISSLPFSTAPFGFFPNPTYPL--PNTT-----FA 393

Query: 375 IFVGKFANVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDLSRCVSGVRKMEDLLKTQTM 434
             V K     S G + L  S++D +  P V FNYYS   DL+ CVSG++K+ + L +  +
Sbjct: 394 HIVNKVPGPLSHGTV-LLQSTSDVRVAPNVTFNYYSNTTDLAHCVSGMKKIGEFLSSDAL 453

Query: 435 ERIKTQDLEGNNGFQFMGLPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVGKVVDDNYK 494
           +  K +DL G  GF  +G+P+PEN  +D++ E +C+  VA+YWHYHGGCLVG+V+DD+++
Sbjct: 454 KPYKVEDLPGIEGFDILGIPLPENQTDDAAFETFCREAVASYWHYHGGCLVGEVLDDDFR 513

Query: 495 VIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVVQQR 527
           V GI  LRVVDGSTF  +P ++P    +MLGRY+G K++Q+R
Sbjct: 514 VTGINALRVVDGSTFPSTPASHPQGFYLMLGRYMGTKILQER 546

BLAST of Chy5G099700 vs. ExPASy TrEMBL
Match: A0A0A0LMB8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G055040 PE=3 SV=1)

HSP 1 Score: 1018.8 bits (2633), Expect = 7.9e-294
Identity = 499/511 (97.65%), Postives = 506/511 (99.02%), Query Frame = 0

Query: 18  ISYMKFVHNAYDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLN 77
           ISYMKFVHNA DQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLN
Sbjct: 29  ISYMKFVHNANDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLN 88

Query: 78  EETLSYVFIIDDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHQEFFETAGVD 137
           EETLSY FI+DDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHQEFFETAGVD
Sbjct: 89  EETLSYTFILDDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHQEFFETAGVD 148

Query: 138 WDMELVEKAYEWVEETLVSRPNLTSWQAAFRNTLLEGGVVPDNGFDLRHLVGTKIGGSIF 197
           WDMELVEKAYEWVEETLVSRPNLT+WQAAFRNTLLEGGVVPDNGFDLRHLVGTKIGGSIF
Sbjct: 149 WDMELVEKAYEWVEETLVSRPNLTAWQAAFRNTLLEGGVVPDNGFDLRHLVGTKIGGSIF 208

Query: 198 DNKGNRHGAVELLNKANPKNLKVVIQATVQRIIFKGLSAVGVLYSDSKGKLHTALIHKKG 257
           DNKGNRHGAVELLNKANPKNLKVVIQATVQRIIFKGLSAVGVLYSDSKGKLHTALIHKKG
Sbjct: 209 DNKGNRHGAVELLNKANPKNLKVVIQATVQRIIFKGLSAVGVLYSDSKGKLHTALIHKKG 268

Query: 258 EIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLHVVHHQPHVGQYMTDNPRFSRSIIFPFQL 317
           EIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLH+VHHQPHVGQYMTDNPRFSRSIIFPFQL
Sbjct: 269 EIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLHLVHHQPHVGQYMTDNPRFSRSIIFPFQL 328

Query: 318 LASTAQVVGTLEKNIHLQSLASPLPFFPLPSYGLLPPQSTSITSSLVIFVGKFANVSSKG 377
           LASTAQVVGTLEKNIHLQSLASPLPFFPLPSYGLLPPQSTSITSSLVI VGKF+NVSSKG
Sbjct: 329 LASTAQVVGTLEKNIHLQSLASPLPFFPLPSYGLLPPQSTSITSSLVIIVGKFSNVSSKG 388

Query: 378 WLHLNNSSTDAKENPIVRFNYYSQHGDLSRCVSGVRKMEDLLKTQTMERIKTQDLEGNNG 437
           WLHLNNSSTDAKENPIVRFNYYSQHGD+SRCVSGVRK+EDLLKTQTMERIKTQDLEGN G
Sbjct: 389 WLHLNNSSTDAKENPIVRFNYYSQHGDISRCVSGVRKVEDLLKTQTMERIKTQDLEGNKG 448

Query: 438 FQFMGLPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGS 497
           FQFM LPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGS
Sbjct: 449 FQFMELPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGS 508

Query: 498 TFSDSPGTNPMATLMMLGRYVGLKVVQQRSS 529
           TFSDSPGTNPMATLMMLGRYVGLKV+QQRSS
Sbjct: 509 TFSDSPGTNPMATLMMLGRYVGLKVLQQRSS 539

BLAST of Chy5G099700 vs. ExPASy TrEMBL
Match: A0A1S3BTE4 ((R)-mandelonitrile lyase 3-like OS=Cucumis melo OX=3656 GN=LOC103493064 PE=3 SV=1)

HSP 1 Score: 996.5 bits (2575), Expect = 4.2e-287
Identity = 484/511 (94.72%), Postives = 501/511 (98.04%), Query Frame = 0

Query: 18  ISYMKFVHNAYDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLN 77
           ISYMKFVHNA DQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLN
Sbjct: 30  ISYMKFVHNANDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLN 89

Query: 78  EETLSYVFIIDDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHQEFFETAGVD 137
           EETLSYVFI++DDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGH+EFFETAGVD
Sbjct: 90  EETLSYVFILEDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHREFFETAGVD 149

Query: 138 WDMELVEKAYEWVEETLVSRPNLTSWQAAFRNTLLEGGVVPDNGFDLRHLVGTKIGGSIF 197
           W+MELVEKAYEWVEETLVSRPNL++WQ AFRNTLLEGGVVPDNGFDLRHL+GTK GGSIF
Sbjct: 150 WEMELVEKAYEWVEETLVSRPNLSAWQVAFRNTLLEGGVVPDNGFDLRHLMGTKTGGSIF 209

Query: 198 DNKGNRHGAVELLNKANPKNLKVVIQATVQRIIFKGLSAVGVLYSDSKGKLHTALIHKKG 257
           DNKGNRHGAVELLNKANPKNLKVVIQATVQRIIF GLSAVGVLYSDSKGKLHTALIHKKG
Sbjct: 210 DNKGNRHGAVELLNKANPKNLKVVIQATVQRIIFNGLSAVGVLYSDSKGKLHTALIHKKG 269

Query: 258 EIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLHVVHHQPHVGQYMTDNPRFSRSIIFPFQL 317
           EI LSAGAIGSPQLLLLSGIGPKSYLSSLKLHVVHHQPHVGQ+MTDNPRFSRSI+FPFQL
Sbjct: 270 EILLSAGAIGSPQLLLLSGIGPKSYLSSLKLHVVHHQPHVGQFMTDNPRFSRSIVFPFQL 329

Query: 318 LASTAQVVGTLEKNIHLQSLASPLPFFPLPSYGLLPPQSTSITSSLVIFVGKFANVSSKG 377
           LAS+AQVVGTLEKNIHLQSLASPLPF+PLPSYGLLPPQSTSIT SLV+FVGKF+NVSSKG
Sbjct: 330 LASSAQVVGTLEKNIHLQSLASPLPFYPLPSYGLLPPQSTSITPSLVVFVGKFSNVSSKG 389

Query: 378 WLHLNNSSTDAKENPIVRFNYYSQHGDLSRCVSGVRKMEDLLKTQTMERIKTQDLEGNNG 437
           WLHLNNSSTD+KE+PIVRFNYYSQH DLSRCV GVRK+EDLLKTQTMER KTQDLEGN G
Sbjct: 390 WLHLNNSSTDSKESPIVRFNYYSQHDDLSRCVRGVRKVEDLLKTQTMERFKTQDLEGNKG 449

Query: 438 FQFMGLPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGS 497
           FQFMGLPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGS
Sbjct: 450 FQFMGLPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGS 509

Query: 498 TFSDSPGTNPMATLMMLGRYVGLKVVQQRSS 529
           TFSDSPGTNPMATLMMLGRYVGLKV+QQRSS
Sbjct: 510 TFSDSPGTNPMATLMMLGRYVGLKVLQQRSS 540

BLAST of Chy5G099700 vs. ExPASy TrEMBL
Match: A0A5D3CI61 ((R)-mandelonitrile lyase 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold231G00870 PE=3 SV=1)

HSP 1 Score: 969.1 bits (2504), Expect = 7.2e-279
Identity = 470/496 (94.76%), Postives = 486/496 (97.98%), Query Frame = 0

Query: 21  MKFVHNAYDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLNEET 80
           MKFVHNA DQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLNEET
Sbjct: 1   MKFVHNANDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLNEET 60

Query: 81  LSYVFIIDDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHQEFFETAGVDWDM 140
           LSYVFI++DDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGH+EFFETAGVDW+M
Sbjct: 61  LSYVFILEDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHREFFETAGVDWEM 120

Query: 141 ELVEKAYEWVEETLVSRPNLTSWQAAFRNTLLEGGVVPDNGFDLRHLVGTKIGGSIFDNK 200
           ELVEKAYEWVEETLVSRPNL++WQ AFRNTLLEGGVVPDNGFDLRHL+GTK GGSIFDNK
Sbjct: 121 ELVEKAYEWVEETLVSRPNLSAWQVAFRNTLLEGGVVPDNGFDLRHLMGTKTGGSIFDNK 180

Query: 201 GNRHGAVELLNKANPKNLKVVIQATVQRIIFKGLSAVGVLYSDSKGKLHTALIHKKGEIF 260
           GNRHGAVELLNKANPKNLKVVIQATVQRIIF GLSAVGVLYSDSKGKLHTALIHKKGEI 
Sbjct: 181 GNRHGAVELLNKANPKNLKVVIQATVQRIIFNGLSAVGVLYSDSKGKLHTALIHKKGEIL 240

Query: 261 LSAGAIGSPQLLLLSGIGPKSYLSSLKLHVVHHQPHVGQYMTDNPRFSRSIIFPFQLLAS 320
           LSAGAIGSPQLLLLSGIGPKSYLSSLKLHVVHHQPHVGQ+MTDNPRFSRSI+FPFQLLAS
Sbjct: 241 LSAGAIGSPQLLLLSGIGPKSYLSSLKLHVVHHQPHVGQFMTDNPRFSRSIVFPFQLLAS 300

Query: 321 TAQVVGTLEKNIHLQSLASPLPFFPLPSYGLLPPQSTSITSSLVIFVGKFANVSSKGWLH 380
           +AQVVGTLEKNIHLQSLASPLPF+PLPSYGLLPPQSTSIT SLV+FVGKF+NVSSKGWLH
Sbjct: 301 SAQVVGTLEKNIHLQSLASPLPFYPLPSYGLLPPQSTSITPSLVVFVGKFSNVSSKGWLH 360

Query: 381 LNNSSTDAKENPIVRFNYYSQHGDLSRCVSGVRKMEDLLKTQTMERIKTQDLEGNNGFQF 440
           LNNSSTD+KE+PIVRFNYYSQH DLSRCV GVRK+EDLLKTQTMER KTQDLEGN GFQF
Sbjct: 361 LNNSSTDSKESPIVRFNYYSQHDDLSRCVRGVRKVEDLLKTQTMERFKTQDLEGNKGFQF 420

Query: 441 MGLPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFS 500
           MGLPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFS
Sbjct: 421 MGLPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFS 480

Query: 501 DSPGTNPMATLMMLGR 517
           DSPGTNPMATLMMLGR
Sbjct: 481 DSPGTNPMATLMMLGR 496

BLAST of Chy5G099700 vs. ExPASy TrEMBL
Match: A0A1S3BTS5 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493179 PE=3 SV=1)

HSP 1 Score: 820.1 bits (2117), Expect = 5.4e-234
Identity = 399/510 (78.24%), Postives = 444/510 (87.06%), Query Frame = 0

Query: 18  ISYMKFVHNAYDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLN 77
           +SYMKFVHNA D P +E+YDYI+IGGGT GCPLATTLSSKFS+L+LERGSDPN YP VLN
Sbjct: 33  VSYMKFVHNATDLPTKEEYDYIIIGGGTAGCPLATTLSSKFSILVLERGSDPNKYPSVLN 92

Query: 78  EETLSYVFIIDDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHQEFFETAGVD 137
            + ++  F   DDG+NP Q FVSEDGVENIRGRVLGGGSMVNAGFYSRGH+EFFE AGVD
Sbjct: 93  AQGINNAFAAGDDGKNPFQYFVSEDGVENIRGRVLGGGSMVNAGFYSRGHREFFENAGVD 152

Query: 138 WDMELVEKAYEWVEETLVSRPNL-TSWQAAFRNTLLEGGVVPDNGFDLRHLVGTKIGGSI 197
           WDMELVEKAYEWVEET+VS+P L  +WQAAFR+ LLEGGVVPDNGFDLRHLVGTKIGGSI
Sbjct: 153 WDMELVEKAYEWVEETVVSQPILNNAWQAAFRSALLEGGVVPDNGFDLRHLVGTKIGGSI 212

Query: 198 FDNKGNRHGAVELLNKANPKNLKVVIQATVQRIIFKGLSAVGVLYSDSKGKLHTALIHKK 257
           FDNKGNRHGAVELLNKANP N+KV IQATVQ+I+F GLSA GV YSDSKGKLHTA IHK 
Sbjct: 213 FDNKGNRHGAVELLNKANPTNIKVAIQATVQKILFSGLSATGVSYSDSKGKLHTASIHKN 272

Query: 258 GEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLHVVHHQPHVGQYMTDNPRFSRSIIFPFQ 317
           GEI +SAGAIGSPQLLLLSGIGPKS+LSSLKL VV HQPHVGQ+MTDNPRF  +I+ PFQ
Sbjct: 273 GEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPHVGQFMTDNPRFGTTIVLPFQ 332

Query: 318 LLASTAQVVGTLEKNIHLQSLASPLPFFPLPSYGLLPPQSTSITSSLVIFVGKFANVSSK 377
           LL S  +VVG L++NI++QS A P  F   P++ LLPPQSTSI  +L  FVGKF++V+SK
Sbjct: 333 LLPSAGKVVGILQENIYIQSFAGPSTFLVPPAFSLLPPQSTSINPTLASFVGKFSDVNSK 392

Query: 378 GWLHLNNSSTDAKENPIVRFNYYSQHGDLSRCVSGVRKMEDLLKTQTMERIKTQDLEGNN 437
           GWLHL NSS D K++PIVRFNYYS H DL RCV GVRK+ DLLKTQT+E+IKTQDLEG  
Sbjct: 393 GWLHL-NSSLDVKKSPIVRFNYYSHHDDLDRCVRGVRKVGDLLKTQTIEKIKTQDLEGEK 452

Query: 438 GFQFMGLPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDG 497
            F F+GLP+ ENLWNDSSV+EYCK TV TYWHYHGGCLVGKVVD NYKVIGI+NLRVVDG
Sbjct: 453 AFMFLGLPLTENLWNDSSVKEYCKKTVTTYWHYHGGCLVGKVVDGNYKVIGIENLRVVDG 512

Query: 498 STFSDSPGTNPMATLMMLGRYVGLKVVQQR 527
           STFS SPGTNPMATLMMLGRYVGLKV++QR
Sbjct: 513 STFSVSPGTNPMATLMMLGRYVGLKVLKQR 541

BLAST of Chy5G099700 vs. ExPASy TrEMBL
Match: A0A5D3CL00 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold231G00890 PE=3 SV=1)

HSP 1 Score: 808.9 bits (2088), Expect = 1.2e-230
Identity = 397/510 (77.84%), Postives = 443/510 (86.86%), Query Frame = 0

Query: 19  SYMKFVHNAYDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLNE 78
           SYMKFVH A +    E+YDYI+IGGGT GCPLA TLSSKFSVLLLERG+DPN YP VLNE
Sbjct: 30  SYMKFVHEASELQESEEYDYIIIGGGTAGCPLAATLSSKFSVLLLERGNDPNKYPSVLNE 89

Query: 79  ETLSYVFIIDDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHQEFFETAGVDW 138
           + L   F+ +DDGQNP Q F+SEDGVEN+RGRVLGGGSM+NAGFYSRGH+EFFETAGVDW
Sbjct: 90  QGLLNAFVAEDDGQNPFQHFISEDGVENLRGRVLGGGSMINAGFYSRGHREFFETAGVDW 149

Query: 139 DMELVEKAYEWVEETLVSRPNLTSWQAAFRNTLLEGGVVPDNGFDLRHLVGTKIGGSIFD 198
           DMELVEKAYEWVEET+VS+P+L+ WQAAFR+ LLEGGV  DNGFDL+HLVGTK GGSIFD
Sbjct: 150 DMELVEKAYEWVEETVVSKPSLSPWQAAFRSALLEGGVGHDNGFDLKHLVGTKTGGSIFD 209

Query: 199 NKGNRHGAVELLNKANPKNLKVVIQATVQRIIFKGLSAVGVLYSDSKGKLHTALIHKKGE 258
           NKGNRHGAVELLNK  P+N KV  QATVQRIIF GLSA GV YSDSKGKLHTA I KKGE
Sbjct: 210 NKGNRHGAVELLNKGEPENFKVATQATVQRIIFTGLSASGVSYSDSKGKLHTAFIRKKGE 269

Query: 259 IFLSAGAIGSPQLLLLSGIGPKSYLSSLKLHVVHHQPHVGQYMTDNPRFSRSIIFPFQLL 318
           I LSAGAIGSPQLLLLSG+GPKS L SLKL VV  QPHVG++M+DNPRF+ +I+ PFQ++
Sbjct: 270 IILSAGAIGSPQLLLLSGVGPKSQLKSLKLPVVLDQPHVGEFMSDNPRFTPTIVLPFQVV 329

Query: 319 ASTAQVVGTLEKNIHLQSLASPLPFFPLPSYGLLPPQSTSITSSLVIFVGKFANVSSKGW 378
           AS+AQVVGTL+ NIHLQ+ ASPLPFF  PS+ LLPPQSTSI  SL IFVGKF++V S+G 
Sbjct: 330 ASSAQVVGTLDNNIHLQAFASPLPFFAPPSFSLLPPQSTSIVPSLAIFVGKFSDVYSEGS 389

Query: 379 LHLNNSSTDAKENPIVRFNYYSQHGDLSRCVSGVRKMEDLLKTQTMERIKTQDLEGNNGF 438
           L L NSS D KE+PIVRFNYYS   DL+RCV GVRK+ DLL+T TME+IKTQDLEGN  F
Sbjct: 390 LRL-NSSIDVKESPIVRFNYYSHPDDLARCVRGVRKVGDLLRTPTMEKIKTQDLEGNKRF 449

Query: 439 QFMGLPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGST 498
           QF+GL +PENL NDS+VEEYC+ TV TYWHYHGGCLVGKVVDDN+KVIGI+NLRVVDGST
Sbjct: 450 QFLGLSLPENLLNDSAVEEYCQKTVTTYWHYHGGCLVGKVVDDNHKVIGIENLRVVDGST 509

Query: 499 FSDSPGTNPMATLMMLGRYVGLKVVQQRSS 529
           FS SPGTNPMATLMMLGRYVGLKV+QQRSS
Sbjct: 510 FSVSPGTNPMATLMMLGRYVGLKVLQQRSS 538

BLAST of Chy5G099700 vs. NCBI nr
Match: XP_011648923.1 ((R)-mandelonitrile lyase 3 [Cucumis sativus] >KGN61116.1 hypothetical protein Csa_021230 [Cucumis sativus])

HSP 1 Score: 1014 bits (2621), Expect = 0.0
Identity = 499/511 (97.65%), Postives = 506/511 (99.02%), Query Frame = 0

Query: 18  ISYMKFVHNAYDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLN 77
           ISYMKFVHNA DQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLN
Sbjct: 29  ISYMKFVHNANDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLN 88

Query: 78  EETLSYVFIIDDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHQEFFETAGVD 137
           EETLSY FI+DDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHQEFFETAGVD
Sbjct: 89  EETLSYTFILDDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHQEFFETAGVD 148

Query: 138 WDMELVEKAYEWVEETLVSRPNLTSWQAAFRNTLLEGGVVPDNGFDLRHLVGTKIGGSIF 197
           WDMELVEKAYEWVEETLVSRPNLT+WQAAFRNTLLEGGVVPDNGFDLRHLVGTKIGGSIF
Sbjct: 149 WDMELVEKAYEWVEETLVSRPNLTAWQAAFRNTLLEGGVVPDNGFDLRHLVGTKIGGSIF 208

Query: 198 DNKGNRHGAVELLNKANPKNLKVVIQATVQRIIFKGLSAVGVLYSDSKGKLHTALIHKKG 257
           DNKGNRHGAVELLNKANPKNLKVVIQATVQRIIFKGLSAVGVLYSDSKGKLHTALIHKKG
Sbjct: 209 DNKGNRHGAVELLNKANPKNLKVVIQATVQRIIFKGLSAVGVLYSDSKGKLHTALIHKKG 268

Query: 258 EIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLHVVHHQPHVGQYMTDNPRFSRSIIFPFQL 317
           EIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLH+VHHQPHVGQYMTDNPRFSRSIIFPFQL
Sbjct: 269 EIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLHLVHHQPHVGQYMTDNPRFSRSIIFPFQL 328

Query: 318 LASTAQVVGTLEKNIHLQSLASPLPFFPLPSYGLLPPQSTSITSSLVIFVGKFANVSSKG 377
           LASTAQVVGTLEKNIHLQSLASPLPFFPLPSYGLLPPQSTSITSSLVI VGKF+NVSSKG
Sbjct: 329 LASTAQVVGTLEKNIHLQSLASPLPFFPLPSYGLLPPQSTSITSSLVIIVGKFSNVSSKG 388

Query: 378 WLHLNNSSTDAKENPIVRFNYYSQHGDLSRCVSGVRKMEDLLKTQTMERIKTQDLEGNNG 437
           WLHLNNSSTDAKENPIVRFNYYSQHGD+SRCVSGVRK+EDLLKTQTMERIKTQDLEGN G
Sbjct: 389 WLHLNNSSTDAKENPIVRFNYYSQHGDISRCVSGVRKVEDLLKTQTMERIKTQDLEGNKG 448

Query: 438 FQFMGLPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGS 497
           FQFM LPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGS
Sbjct: 449 FQFMELPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGS 508

Query: 498 TFSDSPGTNPMATLMMLGRYVGLKVVQQRSS 528
           TFSDSPGTNPMATLMMLGRYVGLKV+QQRSS
Sbjct: 509 TFSDSPGTNPMATLMMLGRYVGLKVLQQRSS 539

BLAST of Chy5G099700 vs. NCBI nr
Match: XP_008451913.1 (PREDICTED: (R)-mandelonitrile lyase 3-like [Cucumis melo])

HSP 1 Score: 991 bits (2563), Expect = 0.0
Identity = 484/511 (94.72%), Postives = 501/511 (98.04%), Query Frame = 0

Query: 18  ISYMKFVHNAYDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLN 77
           ISYMKFVHNA DQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLN
Sbjct: 30  ISYMKFVHNANDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLN 89

Query: 78  EETLSYVFIIDDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHQEFFETAGVD 137
           EETLSYVFI++DDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGH+EFFETAGVD
Sbjct: 90  EETLSYVFILEDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHREFFETAGVD 149

Query: 138 WDMELVEKAYEWVEETLVSRPNLTSWQAAFRNTLLEGGVVPDNGFDLRHLVGTKIGGSIF 197
           W+MELVEKAYEWVEETLVSRPNL++WQ AFRNTLLEGGVVPDNGFDLRHL+GTK GGSIF
Sbjct: 150 WEMELVEKAYEWVEETLVSRPNLSAWQVAFRNTLLEGGVVPDNGFDLRHLMGTKTGGSIF 209

Query: 198 DNKGNRHGAVELLNKANPKNLKVVIQATVQRIIFKGLSAVGVLYSDSKGKLHTALIHKKG 257
           DNKGNRHGAVELLNKANPKNLKVVIQATVQRIIF GLSAVGVLYSDSKGKLHTALIHKKG
Sbjct: 210 DNKGNRHGAVELLNKANPKNLKVVIQATVQRIIFNGLSAVGVLYSDSKGKLHTALIHKKG 269

Query: 258 EIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLHVVHHQPHVGQYMTDNPRFSRSIIFPFQL 317
           EI LSAGAIGSPQLLLLSGIGPKSYLSSLKLHVVHHQPHVGQ+MTDNPRFSRSI+FPFQL
Sbjct: 270 EILLSAGAIGSPQLLLLSGIGPKSYLSSLKLHVVHHQPHVGQFMTDNPRFSRSIVFPFQL 329

Query: 318 LASTAQVVGTLEKNIHLQSLASPLPFFPLPSYGLLPPQSTSITSSLVIFVGKFANVSSKG 377
           LAS+AQVVGTLEKNIHLQSLASPLPF+PLPSYGLLPPQSTSIT SLV+FVGKF+NVSSKG
Sbjct: 330 LASSAQVVGTLEKNIHLQSLASPLPFYPLPSYGLLPPQSTSITPSLVVFVGKFSNVSSKG 389

Query: 378 WLHLNNSSTDAKENPIVRFNYYSQHGDLSRCVSGVRKMEDLLKTQTMERIKTQDLEGNNG 437
           WLHLNNSSTD+KE+PIVRFNYYSQH DLSRCV GVRK+EDLLKTQTMER KTQDLEGN G
Sbjct: 390 WLHLNNSSTDSKESPIVRFNYYSQHDDLSRCVRGVRKVEDLLKTQTMERFKTQDLEGNKG 449

Query: 438 FQFMGLPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGS 497
           FQFMGLPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGS
Sbjct: 450 FQFMGLPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGS 509

Query: 498 TFSDSPGTNPMATLMMLGRYVGLKVVQQRSS 528
           TFSDSPGTNPMATLMMLGRYVGLKV+QQRSS
Sbjct: 510 TFSDSPGTNPMATLMMLGRYVGLKVLQQRSS 540

BLAST of Chy5G099700 vs. NCBI nr
Match: KAA0067297.1 ((R)-mandelonitrile lyase 3-like [Cucumis melo var. makuwa] >TYK10908.1 (R)-mandelonitrile lyase 3-like [Cucumis melo var. makuwa])

HSP 1 Score: 964 bits (2493), Expect = 0.0
Identity = 470/496 (94.76%), Postives = 486/496 (97.98%), Query Frame = 0

Query: 21  MKFVHNAYDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLNEET 80
           MKFVHNA DQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLNEET
Sbjct: 1   MKFVHNANDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLNEET 60

Query: 81  LSYVFIIDDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHQEFFETAGVDWDM 140
           LSYVFI++DDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGH+EFFETAGVDW+M
Sbjct: 61  LSYVFILEDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHREFFETAGVDWEM 120

Query: 141 ELVEKAYEWVEETLVSRPNLTSWQAAFRNTLLEGGVVPDNGFDLRHLVGTKIGGSIFDNK 200
           ELVEKAYEWVEETLVSRPNL++WQ AFRNTLLEGGVVPDNGFDLRHL+GTK GGSIFDNK
Sbjct: 121 ELVEKAYEWVEETLVSRPNLSAWQVAFRNTLLEGGVVPDNGFDLRHLMGTKTGGSIFDNK 180

Query: 201 GNRHGAVELLNKANPKNLKVVIQATVQRIIFKGLSAVGVLYSDSKGKLHTALIHKKGEIF 260
           GNRHGAVELLNKANPKNLKVVIQATVQRIIF GLSAVGVLYSDSKGKLHTALIHKKGEI 
Sbjct: 181 GNRHGAVELLNKANPKNLKVVIQATVQRIIFNGLSAVGVLYSDSKGKLHTALIHKKGEIL 240

Query: 261 LSAGAIGSPQLLLLSGIGPKSYLSSLKLHVVHHQPHVGQYMTDNPRFSRSIIFPFQLLAS 320
           LSAGAIGSPQLLLLSGIGPKSYLSSLKLHVVHHQPHVGQ+MTDNPRFSRSI+FPFQLLAS
Sbjct: 241 LSAGAIGSPQLLLLSGIGPKSYLSSLKLHVVHHQPHVGQFMTDNPRFSRSIVFPFQLLAS 300

Query: 321 TAQVVGTLEKNIHLQSLASPLPFFPLPSYGLLPPQSTSITSSLVIFVGKFANVSSKGWLH 380
           +AQVVGTLEKNIHLQSLASPLPF+PLPSYGLLPPQSTSIT SLV+FVGKF+NVSSKGWLH
Sbjct: 301 SAQVVGTLEKNIHLQSLASPLPFYPLPSYGLLPPQSTSITPSLVVFVGKFSNVSSKGWLH 360

Query: 381 LNNSSTDAKENPIVRFNYYSQHGDLSRCVSGVRKMEDLLKTQTMERIKTQDLEGNNGFQF 440
           LNNSSTD+KE+PIVRFNYYSQH DLSRCV GVRK+EDLLKTQTMER KTQDLEGN GFQF
Sbjct: 361 LNNSSTDSKESPIVRFNYYSQHDDLSRCVRGVRKVEDLLKTQTMERFKTQDLEGNKGFQF 420

Query: 441 MGLPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFS 500
           MGLPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFS
Sbjct: 421 MGLPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFS 480

Query: 501 DSPGTNPMATLMMLGR 516
           DSPGTNPMATLMMLGR
Sbjct: 481 DSPGTNPMATLMMLGR 496

BLAST of Chy5G099700 vs. NCBI nr
Match: XP_038889699.1 ((R)-mandelonitrile lyase 1-like [Benincasa hispida])

HSP 1 Score: 817 bits (2111), Expect = 1.92e-294
Identity = 401/510 (78.63%), Postives = 447/510 (87.65%), Query Frame = 0

Query: 19  SYMKFVHNAYDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLNE 78
           SY+KFVH A + P   +YDYI+IGGGT GCPLA TLSSK+SVLLLERGSDPN YP VLNE
Sbjct: 33  SYLKFVHEANELPETGEYDYIIIGGGTAGCPLAATLSSKYSVLLLERGSDPNKYPSVLNE 92

Query: 79  ETLSYVFIIDDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHQEFFETAGVDW 138
           + L   F+ +DDGQNP Q F+SEDGVENIRGRVLGGGSM+NAGFYSRGH+EFFETAGVDW
Sbjct: 93  QGLLNAFVAEDDGQNPFQHFISEDGVENIRGRVLGGGSMINAGFYSRGHREFFETAGVDW 152

Query: 139 DMELVEKAYEWVEETLVSRPNLTSWQAAFRNTLLEGGVVPDNGFDLRHLVGTKIGGSIFD 198
           DMELVEKAY+WVE+T+VSRP+L++WQ+AFR  LLE GV PDNGFDL+HLVGTK GGSIFD
Sbjct: 153 DMELVEKAYQWVEQTVVSRPSLSAWQSAFRGALLEAGVGPDNGFDLKHLVGTKTGGSIFD 212

Query: 199 NKGNRHGAVELLNKANPKNLKVVIQATVQRIIFKGLSAVGVLYSDSKGKLHTALIHKKGE 258
           NKGNRHGAVELLNKA P+NLKV  QA V+RIIF GLSA GV YSD KGKLHTA I +KGE
Sbjct: 213 NKGNRHGAVELLNKAEPQNLKVGTQAIVERIIFTGLSASGVSYSDPKGKLHTAFIREKGE 272

Query: 259 IFLSAGAIGSPQLLLLSGIGPKSYLSSLKLHVVHHQPHVGQYMTDNPRFSRSIIFPFQLL 318
           I LSAGAIGSPQLLLLSG+GPK +LSSLKL VV HQPHVG++M+DNPRFS +I+ PFQL+
Sbjct: 273 IILSAGAIGSPQLLLLSGVGPKYHLSSLKLPVVLHQPHVGEFMSDNPRFSPTIVLPFQLV 332

Query: 319 ASTAQVVGTLEKNIHLQSLASPLPFFPLPSYGLLPPQSTSITSSLVIFVGKFANVSSKGW 378
           AS+AQVVGTL  NIHLQS ASPLPFF  PS+ LLPPQSTSI  SL IFVGKF++V S+G 
Sbjct: 333 ASSAQVVGTLANNIHLQSFASPLPFFAPPSFSLLPPQSTSIIPSLAIFVGKFSDVLSEGS 392

Query: 379 LHLNNSSTDAKENPIVRFNYYSQHGDLSRCVSGVRKMEDLLKTQTMERIKTQDLEGNNGF 438
           L LN SS D KE+PIVRFNYYS H DL+RCV GVRK+ DLLKTQTME+IK QDLEGN GF
Sbjct: 393 LRLN-SSIDVKESPIVRFNYYSNHDDLARCVRGVRKVGDLLKTQTMEKIKNQDLEGNKGF 452

Query: 439 QFMGLPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGST 498
           QF+GLP+PENLWNDS+VEEYC+ TV TYWHYHGGCLVGKVVD N+KVIGI+NLRVVDGST
Sbjct: 453 QFLGLPLPENLWNDSAVEEYCQKTVTTYWHYHGGCLVGKVVDHNHKVIGIENLRVVDGST 512

Query: 499 FSDSPGTNPMATLMMLGRYVGLKVVQQRSS 528
           FS SPGTNPMATLMMLGRYVGLKV+QQRSS
Sbjct: 513 FSVSPGTNPMATLMMLGRYVGLKVLQQRSS 541

BLAST of Chy5G099700 vs. NCBI nr
Match: XP_008452065.1 (PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo])

HSP 1 Score: 814 bits (2103), Expect = 3.28e-293
Identity = 399/510 (78.24%), Postives = 444/510 (87.06%), Query Frame = 0

Query: 18  ISYMKFVHNAYDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLN 77
           +SYMKFVHNA D P +E+YDYI+IGGGT GCPLATTLSSKFS+L+LERGSDPN YP VLN
Sbjct: 33  VSYMKFVHNATDLPTKEEYDYIIIGGGTAGCPLATTLSSKFSILVLERGSDPNKYPSVLN 92

Query: 78  EETLSYVFIIDDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHQEFFETAGVD 137
            + ++  F   DDG+NP Q FVSEDGVENIRGRVLGGGSMVNAGFYSRGH+EFFE AGVD
Sbjct: 93  AQGINNAFAAGDDGKNPFQYFVSEDGVENIRGRVLGGGSMVNAGFYSRGHREFFENAGVD 152

Query: 138 WDMELVEKAYEWVEETLVSRPNLTS-WQAAFRNTLLEGGVVPDNGFDLRHLVGTKIGGSI 197
           WDMELVEKAYEWVEET+VS+P L + WQAAFR+ LLEGGVVPDNGFDLRHLVGTKIGGSI
Sbjct: 153 WDMELVEKAYEWVEETVVSQPILNNAWQAAFRSALLEGGVVPDNGFDLRHLVGTKIGGSI 212

Query: 198 FDNKGNRHGAVELLNKANPKNLKVVIQATVQRIIFKGLSAVGVLYSDSKGKLHTALIHKK 257
           FDNKGNRHGAVELLNKANP N+KV IQATVQ+I+F GLSA GV YSDSKGKLHTA IHK 
Sbjct: 213 FDNKGNRHGAVELLNKANPTNIKVAIQATVQKILFSGLSATGVSYSDSKGKLHTASIHKN 272

Query: 258 GEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLHVVHHQPHVGQYMTDNPRFSRSIIFPFQ 317
           GEI +SAGAIGSPQLLLLSGIGPKS+LSSLKL VV HQPHVGQ+MTDNPRF  +I+ PFQ
Sbjct: 273 GEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPHVGQFMTDNPRFGTTIVLPFQ 332

Query: 318 LLASTAQVVGTLEKNIHLQSLASPLPFFPLPSYGLLPPQSTSITSSLVIFVGKFANVSSK 377
           LL S  +VVG L++NI++QS A P  F   P++ LLPPQSTSI  +L  FVGKF++V+SK
Sbjct: 333 LLPSAGKVVGILQENIYIQSFAGPSTFLVPPAFSLLPPQSTSINPTLASFVGKFSDVNSK 392

Query: 378 GWLHLNNSSTDAKENPIVRFNYYSQHGDLSRCVSGVRKMEDLLKTQTMERIKTQDLEGNN 437
           GWLHLN SS D K++PIVRFNYYS H DL RCV GVRK+ DLLKTQT+E+IKTQDLEG  
Sbjct: 393 GWLHLN-SSLDVKKSPIVRFNYYSHHDDLDRCVRGVRKVGDLLKTQTIEKIKTQDLEGEK 452

Query: 438 GFQFMGLPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDG 497
            F F+GLP+ ENLWNDSSV+EYCK TV TYWHYHGGCLVGKVVD NYKVIGI+NLRVVDG
Sbjct: 453 AFMFLGLPLTENLWNDSSVKEYCKKTVTTYWHYHGGCLVGKVVDGNYKVIGIENLRVVDG 512

Query: 498 STFSDSPGTNPMATLMMLGRYVGLKVVQQR 526
           STFS SPGTNPMATLMMLGRYVGLKV++QR
Sbjct: 513 STFSVSPGTNPMATLMMLGRYVGLKVLKQR 541

BLAST of Chy5G099700 vs. TAIR 10
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 497.3 bits (1279), Expect = 1.5e-140
Identity = 251/516 (48.64%), Postives = 342/516 (66.28%), Query Frame = 0

Query: 20  YMKFVHNAYDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLNEE 79
           +M+F+ NA D   ++ YDYI++GGGT GCPLA TLS  F VLLLERG  P   P V++ +
Sbjct: 38  FMRFISNATDFASEDYYDYIIVGGGTAGCPLAATLSQSFRVLLLERGGVPYNRPNVMSHD 97

Query: 80  TLSYVFIIDDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHQEFFETAGVDWD 139
                    ++  +P Q F+SE+GV N RGRVLGG S +NAGFYSR  ++FFE +G+ WD
Sbjct: 98  GFLTTLTDVNNFDSPAQSFISEEGVPNARGRVLGGSSAINAGFYSRADKQFFENSGLVWD 157

Query: 140 MELVEKAYEWVEETLVSRPNLTSWQAAFRNTLLEGGVVPDNGFDLRHLVGTKIGGSIFDN 199
           +  V ++YEWVE  +V RP L +WQ A R+ LLE GV P NGF L H VGTKIGGS FD 
Sbjct: 158 LSSVNQSYEWVERAIVFRPQLRTWQTAIRDALLEVGVHPFNGFTLEHKVGTKIGGSTFDR 217

Query: 200 KGNRHGAVELLNKANPKNLKVVIQATVQRIIF--------KGLSAVGVLYSDSKGKLHTA 259
            G RH + +LL  A   N++V + ATV+R++           +SA+GV+Y D  G+ H A
Sbjct: 218 TGRRHSSADLLRYARSSNIRVAVYATVERVLLASSPSVSGSNVSAIGVVYRDQLGRFHHA 277

Query: 260 LIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLHVVHHQPHVGQYMTDNPRFSRSI 319
           LI  +GE+ LSAGA+GSPQLL LSGIGP+SYLS+  + V   QPHVG ++ DNPR   SI
Sbjct: 278 LIRDRGEVILSAGALGSPQLLFLSGIGPRSYLSTWGIPVALDQPHVGDFVYDNPRNGISI 337

Query: 320 IFPFQLLASTAQVVGTLEKNIHLQSLASPLPF-FPLPSYGLLPPQSTSITSSLVIFVGKF 379
           + P  +  S  QVVG  E    L++ ++ +PF  PL S  +  P S  +   +   + K 
Sbjct: 338 VPPVPMENSLIQVVGVTEDGAFLEAASNVIPFASPLHSVFIRAPAS-PLYVPVTTIMEKI 397

Query: 380 ANVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDLSRCVSGVRKMEDLLKTQTMERIKTQ 439
               S G L L  +STD + NP+VRFNY+S   DL RCV+G RK+ ++L+++ M+    +
Sbjct: 398 LGPVSIGLLRL--ASTDVRINPVVRFNYFSDPQDLERCVNGTRKIGEILRSRAMQDFMIR 457

Query: 440 DLEGNNGFQFMGLPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKN 499
           +  GN  F+F+G P+P +  ND  + ++C+ TV+T WHYHGG +VGKVVD + KVIG+ +
Sbjct: 458 EWFGNRRFRFVGAPLPVDQSNDLVMADFCRRTVSTIWHYHGGAVVGKVVDSDLKVIGVNS 517

Query: 500 LRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVVQQR 527
           LR+VDGSTF+ SPGTNP ATLMMLGRY+GLK++++R
Sbjct: 518 LRLVDGSTFNISPGTNPQATLMMLGRYMGLKMLRER 550

BLAST of Chy5G099700 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 402.5 bits (1033), Expect = 5.2e-112
Identity = 232/544 (42.65%), Postives = 314/544 (57.72%), Query Frame = 0

Query: 23  FVHNAYDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLNEETLS 82
           F+ +A   P    YDYI+IGGGT GCPLA TLS   SVLLLERG  P   P +       
Sbjct: 33  FMRDATGSPTTSYYDYIIIGGGTAGCPLAATLSQNASVLLLERGDSPYNNPNITRLSAFG 92

Query: 83  YVFIIDDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHQEFFETAGVDWDMEL 142
              + D    +P QRFVSEDGV N R RVLGGGS +NAGFY+R   ++    G  WD  L
Sbjct: 93  -AALSDLSESSPSQRFVSEDGVINARARVLGGGSALNAGFYTRAGTKYVRNMG--WDGAL 152

Query: 143 VEKAYEWVEETLVSRPNLTSWQAAFRNTLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGN 202
             ++Y+WVE  +  +P +  WQ A R+ LLE G+VP+NGF   H+ GTK GG+IFD  GN
Sbjct: 153 ANESYQWVEAKVAFQPPMGRWQTAVRDGLLEAGIVPNNGFTYDHINGTKFGGTIFDRNGN 212

Query: 203 RHGAVELLNKANPKNLKVVIQATVQRIIFKGLS-----AVGVLYSDSKGKLHTALIHKKG 262
           RH A +LL  A+PK + V++ ATV RI+F+        A GV+Y D  G+ H A + K+G
Sbjct: 213 RHTAADLLEYADPKGITVLLHATVHRILFRTRGTTKPIANGVVYRDRTGQAHRAYL-KEG 272

Query: 263 ---EIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLHVVHHQPHVGQYMTDNPRFSRSIIFP 322
              EI LSAG +GSPQLL+LSG+GP + L +  + VV  QPHVGQ M DNP  +  +  P
Sbjct: 273 ALSEIILSAGTLGSPQLLMLSGVGPSAQLQAQNITVVMDQPHVGQGMYDNPMNAVFVPSP 332

Query: 323 FQLLASTAQVVGTLEKNIHLQSL---------------ASPLPFFPL--PSYGLLPPQST 382
             +  S  +VVG   +  ++++                +S   ++ +  P   LL   S 
Sbjct: 333 VPVEVSLIEVVGITGEGTYVEAAGGENFGGGGGGSSGSSSTRDYYAMFSPRATLLESNSM 392

Query: 383 SITSSLVIFVGKFANVSSKGWL---HLNNSSTDAKENPIVRFNYYSQHGDLSRCVSGVRK 442
           +  SS   F G F      G L   HL   + + K+NPIV FNY+    DL RCV G++ 
Sbjct: 393 TKLSSAQPFQGGFLLEKVMGPLSTGHLELKTRNPKDNPIVTFNYFQHPDDLKRCVRGIQT 452

Query: 443 MEDLLKTQTMERIKTQDLEGNNGFQF---MGLPMPENLWN---------DSSVEEYCKNT 502
           +E +++++   R K  D+     F++   +    P NL             S EE+C++T
Sbjct: 453 IERVVQSKAFSRYKYADV----SFEYLLNLTASTPVNLRPPRSGPGASLPPSAEEFCQHT 512

Query: 503 VATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKV 527
           V T WHYHGGC+VG+VVD +YKVIGI  LRV+D ST    PGTNP AT+MMLGRY+G+K+
Sbjct: 513 VTTIWHYHGGCVVGRVVDGDYKVIGIDRLRVIDMSTVGYCPGTNPQATVMMLGRYMGVKI 568

BLAST of Chy5G099700 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 396.4 bits (1017), Expect = 3.7e-110
Identity = 232/535 (43.36%), Postives = 312/535 (58.32%), Query Frame = 0

Query: 36  YDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNT---YPFVLNEETLSYVFIIDDDGQ 95
           YDYIVIGGGT GCPLA TLS  FSVL+LERG  P T     F+ N     ++ + D    
Sbjct: 63  YDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRN----FHIGLADISAS 122

Query: 96  NPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEE 155
           +  Q FVS DGV N R RVLGGGS +NAGFYSR    F + AG  WD +LV+++Y WVE 
Sbjct: 123 SASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRAG--WDPKLVKESYPWVER 182

Query: 156 TLVSRPNLTSWQAAFRNTLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNK 215
            +V +P LT WQ A R++LLE GV P NGF   H+ GTKIGG+IFD  G RH A ELL  
Sbjct: 183 EIVHQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAELLAY 242

Query: 216 ANPKNLKVVIQATVQRIIFKGLS----AVGVLYSDSKGKLHTALI--HKKGEIFLSAGAI 275
           ANP+ L+V+I ATVQ+I+F          GV++ D KG  H AL+   K  E+ LS+GAI
Sbjct: 243 ANPQKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSSGAI 302

Query: 276 GSPQLLLLSGIGPKSYLSSLKLHVVHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVG 335
           GSPQ+L+LSGIGPK  L  LK+ VV    HVG+ M DNP  +  +     +  S  Q VG
Sbjct: 303 GSPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPMNTILVPSKAPIEQSLIQTVG 362

Query: 336 TLEKNIHLQSLA--SPLPFFPLPSYGLLP---------------PQSTS--ITSSLV--- 395
             +  +++++       P      YG++                P++T   IT +     
Sbjct: 363 ITKMGVYVEASTGFGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITRNKYQLH 422

Query: 396 ------IFVGKFANVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDLSRCVSGVRKMEDL 455
                   + K A   S+G L L N++ D  +NP V FNY+    DL RCV  +R +  +
Sbjct: 423 EAFNGSFILEKLAYPISRGHLSLVNTNVD--DNPSVTFNYFKHPVDLQRCVEAIRLVSKV 482

Query: 456 LKTQTMERIKTQDLEGNNGFQFMGLPM-------PENLWNDSSVEEYCKNTVATYWHYHG 515
           + +     +     +  N  + + L +       P+ L +  S+ ++CK+TV T WHYHG
Sbjct: 483 VTSNRF--LNYTQCDKQNVHKMLSLSVKANINLRPKQLNDTKSMAQFCKDTVVTIWHYHG 542

Query: 516 GCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVVQQR 527
           GCLVGKVV  N KV+G+  LRV+DGSTF +SPGTNP AT+MM+GRY+G+K++++R
Sbjct: 543 GCLVGKVVSPNRKVLGVDRLRVIDGSTFDESPGTNPQATMMMMGRYMGVKILRER 587

BLAST of Chy5G099700 vs. TAIR 10
Match: AT1G14185.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 383.3 bits (983), Expect = 3.2e-106
Identity = 220/503 (43.74%), Postives = 294/503 (58.45%), Query Frame = 0

Query: 34  EKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLNEETLSYVFIIDDDGQN 93
           + +DYIV+GGGT GC LA TLS K+SVL++ERG  P   P V +++   Y  I  D+  +
Sbjct: 35  KSFDYIVVGGGTAGCSLAATLSEKYSVLVIERGGSPFGDPLVEDKKYYGYSLINTDEYSS 94

Query: 94  PIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEET 153
             Q F S DG++N RGRVLGG S +N GFYSR   EF + AG  WD +LV+++Y+WVE  
Sbjct: 95  VAQSFTSVDGIKNHRGRVLGGSSAINGGFYSRASDEFVKKAG--WDKDLVQESYKWVESK 154

Query: 154 LVSRPNLTSWQAAFRNTLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKA 213
           +V  P LT WQ+  +   LE G  P NG+ L H  GTKIGGSIFD  G RH + +LL   
Sbjct: 155 VVFMPELTRWQSIVQFGFLEAGFYPYNGYSLEHTQGTKIGGSIFDQCGKRHTSADLLGYG 214

Query: 214 NPKNLKVVIQATVQRIIFKG--LSAVGVLYSDSKG------KLHTALIHKKGEIFLSAGA 273
            P  + V++ ATV+ IIF      AVGV + +S G      K+H      +GE+ L+AGA
Sbjct: 215 KPNCITVLLNATVKSIIFDANKTRAVGVRFMESDGNSSKSYKVHVE--QHRGEVILAAGA 274

Query: 274 IGSPQLLLLSGIGPKSYLSSLKLHVVHHQPHVGQYMTDNPRFSRSI-IFPFQLLASTAQV 333
           +GSPQ+LLLSGIGP+++L+   + V+ +   VG+ M+DNP  S  +  F   L     QV
Sbjct: 275 LGSPQILLLSGIGPENHLNDFDIPVIVNLKEVGKQMSDNPAISLLVDRFSQNLTVDPPQV 334

Query: 334 VGTLEK-NIHLQSLASPLPFFPLPSYGLLPPQSTSITSSLVIFVGKFANVSSKGWLHLNN 393
           V   E     LQSL  P                T+IT++      K A   SKG L LNN
Sbjct: 335 VAITEGFKFILQSLVLP----------------TNITTTRTAISAKIAFPKSKGRLKLNN 394

Query: 394 SSTDAKENPIVRFNYYSQHGDLSRCVSGVRKMEDLLKTQTMERIKTQDLEGNNGFQFMGL 453
             T+ +ENP V FNY     DL  C       E +L  Q + R KT          F+G 
Sbjct: 395 --TNPRENPSVTFNYLENKADLDAC------QEMVLHLQHVARSKTVTF-------FLGT 454

Query: 454 PMPENL-WNDSSVEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDS 513
              + L   D  ++++C   V TY+HYHGGC+VG VV++ YKV G+K LRVVDGSTF +S
Sbjct: 455 QAQDKLVAGDEELKKFCIKNVRTYYHYHGGCVVGSVVNEEYKVNGVKRLRVVDGSTFEES 502

Query: 514 PGTNPMATLMMLGRYVGLKVVQQ 526
           PGTNPMAT++MLGRY G+K++++
Sbjct: 515 PGTNPMATVLMLGRYQGIKILKE 502

BLAST of Chy5G099700 vs. TAIR 10
Match: AT5G51950.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 381.3 bits (978), Expect = 1.2e-105
Identity = 229/554 (41.34%), Postives = 326/554 (58.84%), Query Frame = 0

Query: 20  YMKFVHNAYDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLNEE 79
           Y  F+ +A   P+  ++DYI+IGGGT+GC LA TLS   SVL+LERG  P   P   + E
Sbjct: 30  YYSFMKDATLAPMFARFDYIIIGGGTSGCALAATLSQNASVLVLERGGAPYDNPTATDIE 89

Query: 80  TLSYVFIIDDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHQEFFETAGVDWD 139
             +   + +   ++  Q F+SEDGV N R RVLGGGS++NAGFY+R   E+ +    +W 
Sbjct: 90  NFA-TTLSNTSPKSWSQLFISEDGVYNTRARVLGGGSVLNAGFYTRAGDEYVKE--TEWK 149

Query: 140 MELVEKAYEWVEETLVSRPNLTSWQAAFRNTLLEGGVVPDNGFDLRHLVGTKIGGSIFDN 199
            + VE AYEWVE+ +  +P +  WQ AF++ LLE G  P NGF   H+ GTKIGG+IFD 
Sbjct: 150 TDEVEAAYEWVEKKVAFQPPVLGWQTAFKDGLLEAGEFPYNGFTYDHIYGTKIGGTIFDR 209

Query: 200 KGNRHGAVELLNKANPKNLKVVIQATVQRIIF--KGL---SAVGVLYSDSKGKLHTALIH 259
            G+RH A +LL  ANP N+ V + A+V +I+F  KG     A GV++ D+ G LH A + 
Sbjct: 210 AGHRHTAADLLEYANPGNIVVYLHASVHKILFTTKGRPRPKAYGVIFQDANGVLHKAELE 269

Query: 260 KK--GEIFLSAGAIGSPQLLLLSGIGPKSYLSS--LKLHVVHHQPHVGQYMTDNPRFSRS 319
           K    E+ LSAGAIGSPQLL+LSGIGP ++L++  +K  V+ H P VGQ M DNP  +  
Sbjct: 270 KNSMNEVILSAGAIGSPQLLMLSGIGPAAHLAAHGIKPLVLDH-PMVGQGMGDNPMNAIF 329

Query: 320 IIFPFQLLASTAQVVGTLEKNIHLQSLASPL-------PFFP-LPSY--GLLPPQSTSIT 379
           I  P  +  S  QVVG  +   +++  +  +        FF  + +Y   +   ++TS T
Sbjct: 330 IPSPTPVEVSLIQVVGITKFESYIEGASGVIFSYSWTRRFFDGVLNYLNEMQTSRTTSTT 389

Query: 380 SSLV-----------------------IFVGKFANVSSKGWLHLNNSSTDAKENPIVRFN 439
           S  +                       + + K A   S+G L L N++ D  +NP VRFN
Sbjct: 390 SPTLSTQSITDFFNPINPLLNATTRAGLILQKIAGPISRGHLELRNTNPD--DNPSVRFN 449

Query: 440 YYSQHGDLSRCVSGVRKMEDLLKTQTMERIKTQDLEGNNGFQFMGLPMPENL-----WND 499
           YY +  DL  CV G+  +  ++ ++   + K  D    +G   + L +P NL      + 
Sbjct: 450 YYQEPEDLQICVEGINTIIKVINSKAFSKFKYPDAT-IHGLLDLMLSVPTNLRPRHITSM 509

Query: 500 SSVEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLM 527
            ++ ++C +TV T WHYHGGC VG+VVD NY+V+GI +LRV+DGSTF  SPGTNP AT+M
Sbjct: 510 FNLRQFCIDTVMTIWHYHGGCQVGRVVDKNYRVLGIDSLRVIDGSTFLKSPGTNPQATVM 569

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P527078.8e-14949.42(R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1[more]
Q945K21.6e-14549.61(R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1[more]
P527062.6e-14549.61(R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1[more]
O242431.3e-14448.84(R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1[more]
O500489.4e-14348.08(R)-mandelonitrile lyase 2 OS=Prunus serotina OX=23207 GN=MDL2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LMB87.9e-29497.65Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G055040 PE=3 SV=1[more]
A0A1S3BTE44.2e-28794.72(R)-mandelonitrile lyase 3-like OS=Cucumis melo OX=3656 GN=LOC103493064 PE=3 SV=... [more]
A0A5D3CI617.2e-27994.76(R)-mandelonitrile lyase 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A1S3BTS55.4e-23478.24(R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493179 PE=3 SV=... [more]
A0A5D3CL001.2e-23077.84(R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
Match NameE-valueIdentityDescription
XP_011648923.10.097.65(R)-mandelonitrile lyase 3 [Cucumis sativus] >KGN61116.1 hypothetical protein Cs... [more]
XP_008451913.10.094.72PREDICTED: (R)-mandelonitrile lyase 3-like [Cucumis melo][more]
KAA0067297.10.094.76(R)-mandelonitrile lyase 3-like [Cucumis melo var. makuwa] >TYK10908.1 (R)-mande... [more]
XP_038889699.11.92e-29478.63(R)-mandelonitrile lyase 1-like [Benincasa hispida][more]
XP_008452065.13.28e-29378.24PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT1G73050.11.5e-14048.64Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G12570.15.2e-11242.65Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G72970.13.7e-11043.36Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G14185.13.2e-10643.74Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT5G51950.11.2e-10541.34Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPFAMPF00732GMC_oxred_Ncoord: 36..305
e-value: 2.1E-32
score: 112.7
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00623GMC_OXRED_1coord: 109..132
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00624GMC_OXRED_2coord: 264..278
IPR012132Glucose-methanol-choline oxidoreductasePIRSFPIRSF000137Alcohol_oxidasecoord: 13..340
e-value: 7.9E-56
score: 187.9
coord: 316..528
e-value: 8.0E-32
score: 108.7
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 36..515
e-value: 8.8E-162
score: 541.4
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 19..523
NoneNo IPR availableGENE3D3.30.410.40coord: 156..465
e-value: 8.8E-162
score: 541.4
NoneNo IPR availablePANTHERPTHR45968OSJNBA0019K04.7 PROTEINcoord: 13..527
NoneNo IPR availablePANTHERPTHR45968:SF23(R)-MANDELONITRILE LYASE 4-RELATEDcoord: 13..527
NoneNo IPR availableSUPERFAMILY54373FAD-linked reductases, C-terminal domaincoord: 360..472
IPR007867Glucose-methanol-choline oxidoreductase, C-terminalPFAMPF05199GMC_oxred_Ccoord: 375..516
e-value: 4.6E-27
score: 95.3

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy5G099700.1Chy5G099700.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016829 lyase activity
molecular_function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors