Chy5G097140 (gene) Cucumber (hystrix) v1

Overview
NameChy5G097140
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionprotein PHYTOCHROME KINASE SUBSTRATE 1
LocationchrH05: 8477695 .. 8479164 (-)
RNA-Seq ExpressionChy5G097140
SyntenyChy5G097140
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACATCTTCACCTCCATATCATCTAAAACATTGCCTTTTGATACCCATATTGATAGCAATAATAATTTGGGAGTTTATGGCGACACCTCCTTCTCGTCTTACTTGTCTGCGAAAGAAGACCATGACTTCATCCGCAAACTCGCTGAGTCAACTCGTTACCTAAAAAGCCCCAACATGATTCCTTGTAGCAGAGGCGGTGAAGATGGAGAGATTGGAATATTTGGAGCCGAAAAGTACTTCAATGGCGGAATGGAAGATGAGAGCACTCAAAGATCAAGTAACAATCATCCATCGTCTCAAAAGCTTGACAAACTTATTGTTGCTCATATGGAGGAAGCCTTGAAATTGCCAAAGCCGAGGCTTGGAACTCCAAGTGTTGGATCGGAATCCAGTAGCGTCAACAGCCAAAGACCTTTGCTGAAGATCGTTAAAAGCACAACAACAACAAGTGCAATAGCAACAACAATTGCCAATAACAGTTATTCGCTCCAGAAGAGGAGTAGTAACAACAACAAGAGCTTTCTATCCAATACTCTTGGCTATTGCATGTGCTGTACAAGCAACAAAAAAAGTACTGCAGTTGAAGACGTGGGTGAAATCAGTTTCAGCAATGCAGTCACAACAAACCCAACACGAAATAATATTAATAATATATTGGATCGAGAAACGCCTTCGTTTCGAGGCTTTCCAACTGCAGCATCATCACTGAAGATGGTCCATTTACAAGAACCTGAAGAAGTAGTTGAAAGAAAGTCGTTAGAGGTTTTCGGTTCGCCTATGACGGGAAGGCATAGAAACAATAAACCAATGAGCCTGGAAAAAAGGCTAACCATGCTTTCTTGGGATAAAACAAATAACCACAGCACCACCTTAGGATCAGGAATGTTTTACAACGAGGACGAGGTGAATAGCGATTGTAGCTCCGACTTGTTTGAGATTGAAAGCCTTACCAAGCAAACCAACCCATTCCACTCTCCAACTGCCTCTTGTTATGCGCCCAGTGAAGCTAGCGTTGACTGGAGCGTCGTCACTGCCAGTGCTCTAGACTTCGACGAACGGCGCCCCTCCACTACCAGTCCAGCCAGAGTGGTGCCACCACCACCAATGAGGGTTAATGTGCACAAAGAGGTTGTCGTTGTCCCGAAGAGGCGACCCGGTAGTATTTTAGGGTGTAAAAGTGAGAAAGCAGTAAGAGTTGCTGAAGGTAATAAGTATGAACGCAAAATGGGTGGTAATAAGTCTAACAACTCTGATTATCTTCTCATGGAACAACGTAACTCCGAGTCGTTAATGGCAGTTAAAAGGTTTGAAGATGAGACCAAAGTCGGAGGGTTCAGTTTCAAATCGCAAGGGTCGTCGTCGATGTTGCCTCTTCCCCTGCCTCTGCCTCATCATGGTCTCGCCACACGCTCGCTTCCTCGCCCTTATTCCCCAAGGCTTACCAATATAACCTTCAATATGCAGTGA

mRNA sequence

ATGGACATCTTCACCTCCATATCATCTAAAACATTGCCTTTTGATACCCATATTGATAGCAATAATAATTTGGGAGTTTATGGCGACACCTCCTTCTCGTCTTACTTGTCTGCGAAAGAAGACCATGACTTCATCCGCAAACTCGCTGAGTCAACTCGTTACCTAAAAAGCCCCAACATGATTCCTTGTAGCAGAGGCGGTGAAGATGGAGAGATTGGAATATTTGGAGCCGAAAAGTACTTCAATGGCGGAATGGAAGATGAGAGCACTCAAAGATCAAGTAACAATCATCCATCGTCTCAAAAGCTTGACAAACTTATTGTTGCTCATATGGAGGAAGCCTTGAAATTGCCAAAGCCGAGGCTTGGAACTCCAAGTGTTGGATCGGAATCCAGTAGCGTCAACAGCCAAAGACCTTTGCTGAAGATCGTTAAAAGCACAACAACAACAAGTGCAATAGCAACAACAATTGCCAATAACAGTTATTCGCTCCAGAAGAGGAGTAGTAACAACAACAAGAGCTTTCTATCCAATACTCTTGGCTATTGCATGTGCTGTACAAGCAACAAAAAAAGTACTGCAGTTGAAGACGTGGGTGAAATCAGTTTCAGCAATGCAGTCACAACAAACCCAACACGAAATAATATTAATAATATATTGGATCGAGAAACGCCTTCGTTTCGAGGCTTTCCAACTGCAGCATCATCACTGAAGATGGTCCATTTACAAGAACCTGAAGAAGTAGTTGAAAGAAAGTCGTTAGAGGTTTTCGGTTCGCCTATGACGGGAAGGCATAGAAACAATAAACCAATGAGCCTGGAAAAAAGGCTAACCATGCTTTCTTGGGATAAAACAAATAACCACAGCACCACCTTAGGATCAGGAATGTTTTACAACGAGGACGAGGTGAATAGCGATTGTAGCTCCGACTTGTTTGAGATTGAAAGCCTTACCAAGCAAACCAACCCATTCCACTCTCCAACTGCCTCTTGTTATGCGCCCAGTGAAGCTAGCGTTGACTGGAGCGTCGTCACTGCCAGTGCTCTAGACTTCGACGAACGGCGCCCCTCCACTACCAGTCCAGCCAGAGTGGTGCCACCACCACCAATGAGGGTTAATGTGCACAAAGAGGTTGTCGTTGTCCCGAAGAGGCGACCCGGTAGTATTTTAGGGTGTAAAAGTGAGAAAGCAGTAAGAGTTGCTGAAGGTAATAAGTATGAACGCAAAATGGGTGGTAATAAGTCTAACAACTCTGATTATCTTCTCATGGAACAACGTAACTCCGAGTCGTTAATGGCAGTTAAAAGGTTTGAAGATGAGACCAAAGTCGGAGGGTTCAGTTTCAAATCGCAAGGGTCGTCGTCGATGTTGCCTCTTCCCCTGCCTCTGCCTCATCATGGTCTCGCCACACGCTCGCTTCCTCGCCCTTATTCCCCAAGGCTTACCAATATAACCTTCAATATGCAGTGA

Coding sequence (CDS)

ATGGACATCTTCACCTCCATATCATCTAAAACATTGCCTTTTGATACCCATATTGATAGCAATAATAATTTGGGAGTTTATGGCGACACCTCCTTCTCGTCTTACTTGTCTGCGAAAGAAGACCATGACTTCATCCGCAAACTCGCTGAGTCAACTCGTTACCTAAAAAGCCCCAACATGATTCCTTGTAGCAGAGGCGGTGAAGATGGAGAGATTGGAATATTTGGAGCCGAAAAGTACTTCAATGGCGGAATGGAAGATGAGAGCACTCAAAGATCAAGTAACAATCATCCATCGTCTCAAAAGCTTGACAAACTTATTGTTGCTCATATGGAGGAAGCCTTGAAATTGCCAAAGCCGAGGCTTGGAACTCCAAGTGTTGGATCGGAATCCAGTAGCGTCAACAGCCAAAGACCTTTGCTGAAGATCGTTAAAAGCACAACAACAACAAGTGCAATAGCAACAACAATTGCCAATAACAGTTATTCGCTCCAGAAGAGGAGTAGTAACAACAACAAGAGCTTTCTATCCAATACTCTTGGCTATTGCATGTGCTGTACAAGCAACAAAAAAAGTACTGCAGTTGAAGACGTGGGTGAAATCAGTTTCAGCAATGCAGTCACAACAAACCCAACACGAAATAATATTAATAATATATTGGATCGAGAAACGCCTTCGTTTCGAGGCTTTCCAACTGCAGCATCATCACTGAAGATGGTCCATTTACAAGAACCTGAAGAAGTAGTTGAAAGAAAGTCGTTAGAGGTTTTCGGTTCGCCTATGACGGGAAGGCATAGAAACAATAAACCAATGAGCCTGGAAAAAAGGCTAACCATGCTTTCTTGGGATAAAACAAATAACCACAGCACCACCTTAGGATCAGGAATGTTTTACAACGAGGACGAGGTGAATAGCGATTGTAGCTCCGACTTGTTTGAGATTGAAAGCCTTACCAAGCAAACCAACCCATTCCACTCTCCAACTGCCTCTTGTTATGCGCCCAGTGAAGCTAGCGTTGACTGGAGCGTCGTCACTGCCAGTGCTCTAGACTTCGACGAACGGCGCCCCTCCACTACCAGTCCAGCCAGAGTGGTGCCACCACCACCAATGAGGGTTAATGTGCACAAAGAGGTTGTCGTTGTCCCGAAGAGGCGACCCGGTAGTATTTTAGGGTGTAAAAGTGAGAAAGCAGTAAGAGTTGCTGAAGGTAATAAGTATGAACGCAAAATGGGTGGTAATAAGTCTAACAACTCTGATTATCTTCTCATGGAACAACGTAACTCCGAGTCGTTAATGGCAGTTAAAAGGTTTGAAGATGAGACCAAAGTCGGAGGGTTCAGTTTCAAATCGCAAGGGTCGTCGTCGATGTTGCCTCTTCCCCTGCCTCTGCCTCATCATGGTCTCGCCACACGCTCGCTTCCTCGCCCTTATTCCCCAAGGCTTACCAATATAACCTTCAATATGCAGTGA

Protein sequence

MDIFTSISSKTLPFDTHIDSNNNLGVYGDTSFSSYLSAKEDHDFIRKLAESTRYLKSPNMIPCSRGGEDGEIGIFGAEKYFNGGMEDESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKPRLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIANNSYSLQKRSSNNNKSFLSNTLGYCMCCTSNKKSTAVEDVGEISFSNAVTTNPTRNNINNILDRETPSFRGFPTAASSLKMVHLQEPEEVVERKSLEVFGSPMTGRHRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYNEDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTTSPARVVPPPPMRVNVHKEVVVVPKRRPGSILGCKSEKAVRVAEGNKYERKMGGNKSNNSDYLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLPLPLPLPHHGLATRSLPRPYSPRLTNITFNMQ*
Homology
BLAST of Chy5G097140 vs. ExPASy Swiss-Prot
Match: Q9SWI1 (Protein PHYTOCHROME KINASE SUBSTRATE 1 OS=Arabidopsis thaliana OX=3702 GN=PKS1 PE=1 SV=2)

HSP 1 Score: 142.9 bits (359), Expect = 9.6e-33
Identity = 155/444 (34.91%), Postives = 217/444 (48.87%), Query Frame = 0

Query: 5   TSISSKTLPFDTHIDSNNNLGVYGDTSFSSYLSAKEDH-DFIRKLAESTRYLK------- 64
           +S S+    FD   ++N++  +Y   S SSYLS+KED     +KL E ++ L        
Sbjct: 7   SSASTPKTSFDFMKNNNSHSSLY--VSSSSYLSSKEDALVTTKKLMEPSKTLNMSINPKQ 66

Query: 65  ---SPNMIPCSRGGEDGEIGIFGAEKYFNGGMEDESTQRSSNNHPSSQKLDKLIVAHMEE 124
                      +  ED EIG+FGAEKYFNG M  +S Q SS    ++ ++++ +V   + 
Sbjct: 67  EEFGDEKKMVKKAPEDPEIGVFGAEKYFNGDM--DSDQGSSVLSLTNPEVERTVVDSKQS 126

Query: 125 ALKLPKPRLGTPSVGSESSSVNSQRPLL--KIVKSTTTTSAIATTIANNSYSLQKRSSNN 184
           A    K   GTPSV SE SS NSQ  LL  K+V S  +    +     NS    ++ +NN
Sbjct: 127 A----KKSTGTPSVRSE-SSWNSQSVLLQNKLVNSCNS----SFKEKKNSNGQIQKVTNN 186

Query: 185 NKSFLSNTLGYCMCCTSNKKSTAVEDVGEISFSNAVTTNPTRNNINNILDRETPSFRGFP 244
            KSFL+N LG C C  S+  S  VE+   +  S     +P   NI+ I  R         
Sbjct: 187 KKSFLAN-LG-CKCACSDGDSVDVEEKTSVKRS----ADP---NISVITMRS-------- 246

Query: 245 TAASSLKMVHLQEPEEVVERKSLEVFGSPMTGRHRNNKPMSLEKRLTMLSWDKTNNHSTT 304
           +A  + +++ +Q+ EE+ +RKSLEVFGSP+       K   ++K+L +  W        T
Sbjct: 247 SADMNTELIKIQKQEELSQRKSLEVFGSPVA---IEKKSSVVQKKLPLPPWKSRTEEDDT 306

Query: 305 LGSGMFYNEDEVNSDCSSDLFEIESLTKQTNPF--------HSPTASCYAPSEASVDWSV 364
              G         SD SSDLFEIE LT    PF         SPT  CYAPSE SV+WS+
Sbjct: 307 KSEG---------SDSSSDLFEIEGLTGNPKPFLTRQGSDPASPT--CYAPSEVSVEWSI 366

Query: 365 VTASALDFDERRPSTTSPARVVPPPPM-RVNVHKEVVVVPKRRP--------GSILGCKS 419
           VTASA DF       TSP R   P  + R+ +  +    P+RR         G ++ CKS
Sbjct: 367 VTASAADFSVMSECATSPVRRNRPTQIPRIPITAK--SAPQRRKSSSSSGGNGFLMSCKS 402

BLAST of Chy5G097140 vs. ExPASy Swiss-Prot
Match: Q9M9T4 (Protein PHYTOCHROME KINASE SUBSTRATE 2 OS=Arabidopsis thaliana OX=3702 GN=PKS2 PE=1 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 6.5e-29
Identity = 149/437 (34.10%), Postives = 212/437 (48.51%), Query Frame = 0

Query: 5   TSISSKTLPFDTHIDSNNNL-GVYGDTSFS----SYLSAKEDHDFIRKLAES-TRYLKSP 64
           +S S+  + FD  +++NNN   +YG  S S    SYL++KED    + L    T  +   
Sbjct: 6   SSSSTPNVSFDFMMNNNNNSNNLYGPFSSSSTSFSYLTSKEDALTQKNLMSGITNDVLGI 65

Query: 65  NMIPCSRGGEDGEIGIFGAEKYFNGGMEDESTQRSSNNHPSSQ-KLDKLIVAHMEEALKL 124
           N     +  ED EI +FGAEKYFNG M+ + + R  +  P  +  ++++ V   + +   
Sbjct: 66  N----KKASEDLEISVFGAEKYFNGDMDSDHSPRLVSPLPDPEVPIERIFVGPKQSS--- 125

Query: 125 PKPRLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIANNSYSLQKRSSNNN----- 184
            K    TPS+ SE SS NSQ  LL+  K       I    + NSY  +K  S+N+     
Sbjct: 126 -KNSSETPSLRSE-SSWNSQSLLLQ-SKYVEKKKNIKKNSSCNSYFQEKDMSSNHKVSNK 185

Query: 185 KSFLSNTLGYCMCCTSNKKST-AVEDVGEISFSNAVTTNPTRNNINNILDRETPSFRGFP 244
           KSFL+ TLG C C  SN  S   V+D    S    + T                    F 
Sbjct: 186 KSFLA-TLG-CRCVCSNWSSVDVVDDKRRSSGLKKIKTQ-----------------LSFS 245

Query: 245 TAASSLKMVHLQEPEEVVE-RKSLEVFGSPMTGRHRNNKPMSLEKRLTM--LSWDKTNN- 304
              SS   +H Q+ E ++E RKSLE+FGSP+           +EKR+      W+ +++ 
Sbjct: 246 GDLSSEMKIHQQQQEAMLEQRKSLEIFGSPL-----------IEKRIIQKKFPWEYSSSA 305

Query: 305 HSTTLGSGMFYNEDEVN--SDCSSDLFEIESLTKQTNPFHSPTASC-------YAPSEAS 364
                G  + Y E+E    SD S+DLFEIESLT + NPF +   S        YAPSE S
Sbjct: 306 KKEEHGFSVKYEEEEDGSVSDVSTDLFEIESLTGKANPFLARQGSSDPDSPDGYAPSEVS 365

Query: 365 VDWSVVTASALDFDERRPSTTSPAR-----VVPPPPMRVNVHKEVVVVPKRR----PGSI 407
           + WSVVTAS  DF       TSP +      +P  P+    ++E  + P+RR     G +
Sbjct: 366 IQWSVVTASVADFSVMSECATSPVKKNRSFQIPRIPIMAKSNRE--IAPQRRKSSSSGLL 399

BLAST of Chy5G097140 vs. ExPASy Swiss-Prot
Match: Q8GXS8 (Protein PHYTOCHROME KINASE SUBSTRATE 3 OS=Arabidopsis thaliana OX=3702 GN=PKS3 PE=2 SV=2)

HSP 1 Score: 71.2 bits (173), Expect = 3.6e-11
Identity = 107/376 (28.46%), Postives = 149/376 (39.63%), Query Frame = 0

Query: 65  RGGEDGEIGIFGAEKYFNGGMED-ESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKP--- 124
           +   D EIG+FGAEKYF+  ++  +ST   +  H      D             P P   
Sbjct: 41  KADNDAEIGVFGAEKYFSMKLDHVDSTADITKQHEKENTHDHPNANPNPNPNPHPHPHPQ 100

Query: 125 ------------RLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIANNSYSLQKRS 184
                       R GTPSV SE SS NSQ  L++I               NN    ++R 
Sbjct: 101 LTKTTSSRSKTSRHGTPSVRSE-SSCNSQTFLMRI---------------NNHNENKQRK 160

Query: 185 SNNNKSFLSNTLGYCMCCTSNKKSTAVEDVGEISFSNAVTTNPTRNNINNILDRETPSFR 244
            N+     S + G   C             G  S    V T+P  +  +   DR+  ++ 
Sbjct: 161 MNDT----SISFGGFRC------------YGPCSGVKTVHTDPKNSCKSRNSDRDFVAYD 220

Query: 245 GFPTAASSLKMVHLQEPEEVVERKSLEVFGSPMTGR--HRNNKPMSLEKRLTMLSWD--- 304
                       H  +P    E K  +            R++  M+LE++L++L+WD   
Sbjct: 221 A---------RKHNDKPRLHFEAKKADFHAQEKISLPIQRSDIAMNLERKLSLLTWDAIP 280

Query: 305 -----KTNNHSTT-LGSGMFYN--EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPS 364
                K NNH+     S M  N  E+E  S  SSDLFEIE++          T+S Y PS
Sbjct: 281 NQLSTKNNNHNNNGNNSSMSSNTQEEETASVASSDLFEIENI----------TSSVYEPS 340

Query: 365 EASVDWSVVTASAL------DFDERRPSTTSPARVVPPPPMRVNVHKEVVVVPKRRPGSI 406
           EAS+ WSVVT S        DFD  +  T +   V   P         V+    R  G +
Sbjct: 341 EASIGWSVVTGSMADQSVISDFDMMKRVTRNGPVVKTKP---------VIGEKVRSAGFL 356

BLAST of Chy5G097140 vs. ExPASy TrEMBL
Match: A0A0A0LKV2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G108600 PE=4 SV=1)

HSP 1 Score: 936.4 bits (2419), Expect = 4.8e-269
Identity = 481/490 (98.16%), Postives = 485/490 (98.98%), Query Frame = 0

Query: 1   MDIFTSISSKTLPFDTHIDSNNNLGVYGDTSFSSYLSAKEDHDFIRKLAESTRYLKSPNM 60
           MDIFTSISSKTLPFDTHIDSNN+LGVYGDTSFSSYLSAKEDHDFIRKLAESTRYLKSPNM
Sbjct: 1   MDIFTSISSKTLPFDTHIDSNNSLGVYGDTSFSSYLSAKEDHDFIRKLAESTRYLKSPNM 60

Query: 61  IPCSRGGEDGEIGIFGAEKYFNGGMEDESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKP 120
           IPCSRGGEDGEIGIFGAEKYFN GMEDESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKP
Sbjct: 61  IPCSRGGEDGEIGIFGAEKYFNDGMEDESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKP 120

Query: 121 RLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIANNSYSLQKRSSNNNKSFLSNTL 180
           RLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIA+NSYSLQKRSSNNNKSFLSNTL
Sbjct: 121 RLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKRSSNNNKSFLSNTL 180

Query: 181 GYCMCCTSNKKSTAVEDVGEISFSNAVTTNPTRNNINNILDRETPSFRGFPTAASSLKMV 240
           GYCMCCTSNKKSTAVEDVGEISFSNA+TTNPTRNNINNILDRE PSFRGFPTAASSLKMV
Sbjct: 181 GYCMCCTSNKKSTAVEDVGEISFSNAITTNPTRNNINNILDREAPSFRGFPTAASSLKMV 240

Query: 241 HLQEPEEVVERKSLEVFGSPMTGRHRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYNE 300
           HLQEPEEVVERKSLEVFGSPMTGR RNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYNE
Sbjct: 241 HLQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYNE 300

Query: 301 DEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTTS 360
           DEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTTS
Sbjct: 301 DEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTTS 360

Query: 361 PARVVPPPPMRVNVHKEVVVVPKRRPGSILGCKSEKAVRVAEG-NKYERKMGGNKSNNSD 420
           PARVVPPPPMRVNVHKEVVVVPKRRP SILGCKSEKAVRVAEG NKYERKMGGNKSNNSD
Sbjct: 361 PARVVPPPPMRVNVHKEVVVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMGGNKSNNSD 420

Query: 421 YLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLPLPLPLPHHGLATRSLPRPYSP 480
           YLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLP+PLPLPHHGLATRSLPRPYSP
Sbjct: 421 YLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLPIPLPLPHHGLATRSLPRPYSP 480

Query: 481 RLTNITFNMQ 490
           RLTNITFNMQ
Sbjct: 481 RLTNITFNMQ 490

BLAST of Chy5G097140 vs. ExPASy TrEMBL
Match: A0A5A7SNC2 (Protein PHYTOCHROME KINASE SUBSTRATE 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold745G00080 PE=4 SV=1)

HSP 1 Score: 875.5 bits (2261), Expect = 1.0e-250
Identity = 458/491 (93.28%), Postives = 467/491 (95.11%), Query Frame = 0

Query: 1   MDIFTSISSKTLPFDTHIDSNNNLGVYGDTSFSSYLSAKEDHDFIRKLAESTRYLKSPNM 60
           MDIFTSISSKTLPFDTHIDSNNNLGVYGDTSFSSYL+AKEDHDFIRKL ESTRYLKSP M
Sbjct: 1   MDIFTSISSKTLPFDTHIDSNNNLGVYGDTSFSSYLTAKEDHDFIRKLTESTRYLKSPIM 60

Query: 61  IPCSRGGEDGEIGIFGAEKYFNGGMEDESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKP 120
           IP SRGGEDGEIGIFGAEKYFNGGMEDESTQRS NNHPSSQK DKLIVAHMEEALKLPKP
Sbjct: 61  IPGSRGGEDGEIGIFGAEKYFNGGMEDESTQRSGNNHPSSQKFDKLIVAHMEEALKLPKP 120

Query: 121 RLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIANNSYSLQK-RSSNNNKSFLSNT 180
           RLGTPSVGSESSSVNSQRPLLKIVKSTTTT+AIATTIANNSYSLQK RSSNNNKSFLSNT
Sbjct: 121 RLGTPSVGSESSSVNSQRPLLKIVKSTTTTTAIATTIANNSYSLQKRRSSNNNKSFLSNT 180

Query: 181 LGYCMCCTSNKKSTAVEDVGEISFSNAVTTNPTRNNINNILDRETPSFRGFPTAASSLKM 240
           LGYCMCCTSNKKSTAVEDVGEISFSNAVTTNPTRNNINNILDRETPSFRGFPTAASS+KM
Sbjct: 181 LGYCMCCTSNKKSTAVEDVGEISFSNAVTTNPTRNNINNILDRETPSFRGFPTAASSMKM 240

Query: 241 VHLQEPEEVVERKSLEVFGSPMTGRHRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYN 300
           VHLQEPEEVVERKSLEVFGSP+TGR R+NKP+SLEKRLTMLSWDKTNNHSTTLGSGMFYN
Sbjct: 241 VHLQEPEEVVERKSLEVFGSPVTGRLRSNKPISLEKRLTMLSWDKTNNHSTTLGSGMFYN 300

Query: 301 EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTT 360
           EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPS T
Sbjct: 301 EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSIT 360

Query: 361 SPARVVPPPPMRVNVHKEVVVVPKRRPGSILGCKSEKAVRVAEG-NKYERKMGGNKSNNS 420
           SPARVVPPPPMRVNVHKEV VVPKRRP SILGC SEKAVRVAEG NKY+ KM GNKSNNS
Sbjct: 361 SPARVVPPPPMRVNVHKEVFVVPKRRPSSILGCNSEKAVRVAEGNNKYDHKMSGNKSNNS 420

Query: 421 DYLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLPLPLPLPHHGLATRSLPRPYS 480
           DYL MEQR+SESLMAVKRFEDETKVGG SFKSQGSSSMLPL  P     L TRSLPRPYS
Sbjct: 421 DYLHMEQRDSESLMAVKRFEDETKVGGLSFKSQGSSSMLPLAPPA--RALGTRSLPRPYS 480

Query: 481 PRLTNITFNMQ 490
           PRLTN+TFNMQ
Sbjct: 481 PRLTNMTFNMQ 489

BLAST of Chy5G097140 vs. ExPASy TrEMBL
Match: A0A1S3B4P0 (protein PHYTOCHROME KINASE SUBSTRATE 1 OS=Cucumis melo OX=3656 GN=LOC103485779 PE=4 SV=1)

HSP 1 Score: 875.5 bits (2261), Expect = 1.0e-250
Identity = 458/491 (93.28%), Postives = 467/491 (95.11%), Query Frame = 0

Query: 1   MDIFTSISSKTLPFDTHIDSNNNLGVYGDTSFSSYLSAKEDHDFIRKLAESTRYLKSPNM 60
           MDIFTSISSKTLPFDTHIDSNNNLGVYGDTSFSSYL+AKEDHDFIRKL ESTRYLKSP M
Sbjct: 1   MDIFTSISSKTLPFDTHIDSNNNLGVYGDTSFSSYLTAKEDHDFIRKLTESTRYLKSPIM 60

Query: 61  IPCSRGGEDGEIGIFGAEKYFNGGMEDESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKP 120
           IP SRGGEDGEIGIFGAEKYFNGGMEDESTQRS NNHPSSQK DKLIVAHMEEALKLPKP
Sbjct: 61  IPGSRGGEDGEIGIFGAEKYFNGGMEDESTQRSGNNHPSSQKFDKLIVAHMEEALKLPKP 120

Query: 121 RLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIANNSYSLQK-RSSNNNKSFLSNT 180
           RLGTPSVGSESSSVNSQRPLLKIVKSTTTT+AIATTIANNSYSLQK RSSNNNKSFLSNT
Sbjct: 121 RLGTPSVGSESSSVNSQRPLLKIVKSTTTTTAIATTIANNSYSLQKRRSSNNNKSFLSNT 180

Query: 181 LGYCMCCTSNKKSTAVEDVGEISFSNAVTTNPTRNNINNILDRETPSFRGFPTAASSLKM 240
           LGYCMCCTSNKKSTAVEDVGEISFSNAVTTNPTRNNINNILDRETPSFRGFPTAASS+KM
Sbjct: 181 LGYCMCCTSNKKSTAVEDVGEISFSNAVTTNPTRNNINNILDRETPSFRGFPTAASSMKM 240

Query: 241 VHLQEPEEVVERKSLEVFGSPMTGRHRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYN 300
           VHLQEPEEVVERKSLEVFGSP+TGR R+NKP+SLEKRLTMLSWDKTNNHSTTLGSGMFYN
Sbjct: 241 VHLQEPEEVVERKSLEVFGSPVTGRLRSNKPISLEKRLTMLSWDKTNNHSTTLGSGMFYN 300

Query: 301 EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTT 360
           EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPS T
Sbjct: 301 EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSIT 360

Query: 361 SPARVVPPPPMRVNVHKEVVVVPKRRPGSILGCKSEKAVRVAEG-NKYERKMGGNKSNNS 420
           SPARVVPPPPMRVNVHKEV VVPKRRP SILGC SEKAVRVAEG NKY+ KM GNKSNNS
Sbjct: 361 SPARVVPPPPMRVNVHKEVFVVPKRRPSSILGCNSEKAVRVAEGNNKYDHKMSGNKSNNS 420

Query: 421 DYLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLPLPLPLPHHGLATRSLPRPYS 480
           DYL MEQR+SESLMAVKRFEDETKVGG SFKSQGSSSMLPL  P     L TRSLPRPYS
Sbjct: 421 DYLHMEQRDSESLMAVKRFEDETKVGGLSFKSQGSSSMLPLAPPA--RALGTRSLPRPYS 480

Query: 481 PRLTNITFNMQ 490
           PRLTN+TFNMQ
Sbjct: 481 PRLTNMTFNMQ 489

BLAST of Chy5G097140 vs. ExPASy TrEMBL
Match: A0A6J1K8F0 (protein PHYTOCHROME KINASE SUBSTRATE 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493179 PE=4 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 1.6e-94
Identity = 235/429 (54.78%), Postives = 273/429 (63.64%), Query Frame = 0

Query: 70  GEIGIFGAEKYFNGGME---DESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKPRLGTPS 129
           GEIG+F AEKYFNGG++   D       NN P S+ LD        +    P P L TPS
Sbjct: 16  GEIGVFRAEKYFNGGLDIHVDSPRINPPNNKP-SKNLD-------PDGPFTPNPWLATPS 75

Query: 130 VGSES--SSVNSQRPLLKIVKSTTTTSAIATTIANNSYSLQKRSSNNNKSFLSNTLG-YC 189
           V SES  ++ N+Q+PLL   K                       ++N K FLS+ LG YC
Sbjct: 76  VASESCTTTTNTQKPLLNTHK-----------------------THNTKGFLSHALGYYC 135

Query: 190 MCCTSNKKSTAVEDVGEISFSNAVTTNPTRNNINNILDRETPSFRGFPTAASSLKMVHLQ 249
           MC + + K T  +D+GEISFS  VTT PT +  +N           F     SLK+VH Q
Sbjct: 136 MCYSRSDKPT--DDIGEISFSKPVTTAPTASFRDN----------SFTFQNGSLKLVHFQ 195

Query: 250 EPEEVVERKSLEVFGSPMTGRHRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYNEDEV 309
           EPE  VERKSLEVFGSP+ GRHR NKP SLEK+L MLSWD TN+ S         N DE 
Sbjct: 196 EPE--VERKSLEVFGSPVIGRHR-NKPFSLEKKLAMLSWDHTNSSSNN-------NNDEA 255

Query: 310 NSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTTSPAR 369
           NSDCSSDLFEIESLTKQTNPF SPT S YAPSEASV+WSVVTASALDFDERR STTSPAR
Sbjct: 256 NSDCSSDLFEIESLTKQTNPFQSPTPSYYAPSEASVEWSVVTASALDFDERRISTTSPAR 315

Query: 370 VV-PPPPMRVNVHKEV-VVVPKRRPGSILGCKSEKAVRVAEGNKYERKMGGNKSNNSDYL 429
           V  PPPP RV V+++V VVV KRR GS+LGCKSEKAVRVAE N   RK+ G  + NS   
Sbjct: 316 VAPPPPPRRVGVNEQVAVVVQKRRSGSLLGCKSEKAVRVAEDNSNGRKISGKVNFNSSSS 375

Query: 430 LMEQRNSESL-MAVKRFEDETKVGGFSFKSQGSSSMLPLPLPLPHHGLATRSLPRPYSPR 489
            +      S+ ++V R EDET+V  FSF+SQ    +    LP  HH LATRSLPRPYSPR
Sbjct: 376 SVSVSVPVSVPVSVNRLEDETRVRSFSFRSQAPQPL----LPSSHHALATRSLPRPYSPR 387

BLAST of Chy5G097140 vs. ExPASy TrEMBL
Match: A0A6J1GCF6 (protein PHYTOCHROME KINASE SUBSTRATE 1-like OS=Cucurbita moschata OX=3662 GN=LOC111452711 PE=4 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 2.1e-91
Identity = 230/428 (53.74%), Postives = 267/428 (62.38%), Query Frame = 0

Query: 70  GEIGIFGAEKYFNGGME---DESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKPRLGTPS 129
           GEIG+F AEKYFNGG++   D       NN PS        +    +    P P L TPS
Sbjct: 16  GEIGVFRAEKYFNGGLDIHVDSPRTNPPNNKPS--------INLAPDEPFTPNPWLATPS 75

Query: 130 VGSESSSVNSQRPLLKIVKSTTTTSAIATTIANNSYSLQKRSSNNNKSFLSNTLG-YCMC 189
           V SES               TTTT+     + N         ++N K FLS+ LG YCMC
Sbjct: 76  VASES--------------CTTTTTTTQKPLLNT------HKTHNTKGFLSHALGYYCMC 135

Query: 190 CTSNKKSTAVEDVGEISFSNAVTTNPTRNNINNILDRETPSFRGFPTAASSLKMVHLQEP 249
            + + K +  +D+GEISFS  VTT PT +  +N           F     SLK+VH QEP
Sbjct: 136 YSRSDKPS--DDIGEISFSRPVTTAPTASFRDN----------SFTFQNGSLKLVHFQEP 195

Query: 250 EEVVERKSLEVFGSPMTGRHRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYNEDEVNS 309
           E  VERKSLEVFGSP+ GRHR NKP SLEK+L MLSWD TNN++        YN DE NS
Sbjct: 196 E--VERKSLEVFGSPVIGRHR-NKPFSLEKKLAMLSWDHTNNNN--------YNNDEANS 255

Query: 310 DCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTTSPARVV 369
           DCSSDLFEIESLTKQTNPF SPT S YAPSEASV+WSVVTASALDFDERR STTSPARV 
Sbjct: 256 DCSSDLFEIESLTKQTNPFQSPTPSYYAPSEASVEWSVVTASALDFDERRISTTSPARVA 315

Query: 370 ---PPPPMRVNVHKEV-VVVPKRRPGSILGCKSEKAVRVAEGNKYERKMGGNKS-NNSDY 429
              PPPP RV V+++V VVV KRR GS+LGCKSEKAVRVAE N   RK+ G  + N+S  
Sbjct: 316 APPPPPPRRVGVNEQVAVVVQKRRSGSLLGCKSEKAVRVAEDNSNGRKISGKVNFNSSSS 375

Query: 430 LLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLPLPLPLPHHGLATRSLPRPYSPR 489
            +         ++V RFEDET+V  FSF+SQ      P  LP   H LATRSLPRPYSPR
Sbjct: 376 SVSVSVPVPVSVSVNRFEDETRVRSFSFRSQAP----PPLLPSSRHSLATRSLPRPYSPR 388

BLAST of Chy5G097140 vs. NCBI nr
Match: XP_004138989.1 (protein PHYTOCHROME KINASE SUBSTRATE 1 [Cucumis sativus] >KGN61382.1 hypothetical protein Csa_006559 [Cucumis sativus])

HSP 1 Score: 932 bits (2409), Expect = 0.0
Identity = 481/490 (98.16%), Postives = 485/490 (98.98%), Query Frame = 0

Query: 1   MDIFTSISSKTLPFDTHIDSNNNLGVYGDTSFSSYLSAKEDHDFIRKLAESTRYLKSPNM 60
           MDIFTSISSKTLPFDTHIDSNN+LGVYGDTSFSSYLSAKEDHDFIRKLAESTRYLKSPNM
Sbjct: 1   MDIFTSISSKTLPFDTHIDSNNSLGVYGDTSFSSYLSAKEDHDFIRKLAESTRYLKSPNM 60

Query: 61  IPCSRGGEDGEIGIFGAEKYFNGGMEDESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKP 120
           IPCSRGGEDGEIGIFGAEKYFN GMEDESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKP
Sbjct: 61  IPCSRGGEDGEIGIFGAEKYFNDGMEDESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKP 120

Query: 121 RLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIANNSYSLQKRSSNNNKSFLSNTL 180
           RLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIA+NSYSLQKRSSNNNKSFLSNTL
Sbjct: 121 RLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKRSSNNNKSFLSNTL 180

Query: 181 GYCMCCTSNKKSTAVEDVGEISFSNAVTTNPTRNNINNILDRETPSFRGFPTAASSLKMV 240
           GYCMCCTSNKKSTAVEDVGEISFSNA+TTNPTRNNINNILDRE PSFRGFPTAASSLKMV
Sbjct: 181 GYCMCCTSNKKSTAVEDVGEISFSNAITTNPTRNNINNILDREAPSFRGFPTAASSLKMV 240

Query: 241 HLQEPEEVVERKSLEVFGSPMTGRHRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYNE 300
           HLQEPEEVVERKSLEVFGSPMTGR RNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYNE
Sbjct: 241 HLQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYNE 300

Query: 301 DEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTTS 360
           DEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTTS
Sbjct: 301 DEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTTS 360

Query: 361 PARVVPPPPMRVNVHKEVVVVPKRRPGSILGCKSEKAVRVAEGN-KYERKMGGNKSNNSD 420
           PARVVPPPPMRVNVHKEVVVVPKRRP SILGCKSEKAVRVAEGN KYERKMGGNKSNNSD
Sbjct: 361 PARVVPPPPMRVNVHKEVVVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMGGNKSNNSD 420

Query: 421 YLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLPLPLPLPHHGLATRSLPRPYSP 480
           YLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLP+PLPLPHHGLATRSLPRPYSP
Sbjct: 421 YLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLPIPLPLPHHGLATRSLPRPYSP 480

Query: 481 RLTNITFNMQ 489
           RLTNITFNMQ
Sbjct: 481 RLTNITFNMQ 490

BLAST of Chy5G097140 vs. NCBI nr
Match: XP_008441702.1 (PREDICTED: protein PHYTOCHROME KINASE SUBSTRATE 1 [Cucumis melo] >KAA0032290.1 protein PHYTOCHROME KINASE SUBSTRATE 1 [Cucumis melo var. makuwa] >TYK13512.1 protein PHYTOCHROME KINASE SUBSTRATE 1 [Cucumis melo var. makuwa])

HSP 1 Score: 872 bits (2252), Expect = 0.0
Identity = 458/491 (93.28%), Postives = 467/491 (95.11%), Query Frame = 0

Query: 1   MDIFTSISSKTLPFDTHIDSNNNLGVYGDTSFSSYLSAKEDHDFIRKLAESTRYLKSPNM 60
           MDIFTSISSKTLPFDTHIDSNNNLGVYGDTSFSSYL+AKEDHDFIRKL ESTRYLKSP M
Sbjct: 1   MDIFTSISSKTLPFDTHIDSNNNLGVYGDTSFSSYLTAKEDHDFIRKLTESTRYLKSPIM 60

Query: 61  IPCSRGGEDGEIGIFGAEKYFNGGMEDESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKP 120
           IP SRGGEDGEIGIFGAEKYFNGGMEDESTQRS NNHPSSQK DKLIVAHMEEALKLPKP
Sbjct: 61  IPGSRGGEDGEIGIFGAEKYFNGGMEDESTQRSGNNHPSSQKFDKLIVAHMEEALKLPKP 120

Query: 121 RLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIANNSYSLQKR-SSNNNKSFLSNT 180
           RLGTPSVGSESSSVNSQRPLLKIVKSTTTT+AIATTIANNSYSLQKR SSNNNKSFLSNT
Sbjct: 121 RLGTPSVGSESSSVNSQRPLLKIVKSTTTTTAIATTIANNSYSLQKRRSSNNNKSFLSNT 180

Query: 181 LGYCMCCTSNKKSTAVEDVGEISFSNAVTTNPTRNNINNILDRETPSFRGFPTAASSLKM 240
           LGYCMCCTSNKKSTAVEDVGEISFSNAVTTNPTRNNINNILDRETPSFRGFPTAASS+KM
Sbjct: 181 LGYCMCCTSNKKSTAVEDVGEISFSNAVTTNPTRNNINNILDRETPSFRGFPTAASSMKM 240

Query: 241 VHLQEPEEVVERKSLEVFGSPMTGRHRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYN 300
           VHLQEPEEVVERKSLEVFGSP+TGR R+NKP+SLEKRLTMLSWDKTNNHSTTLGSGMFYN
Sbjct: 241 VHLQEPEEVVERKSLEVFGSPVTGRLRSNKPISLEKRLTMLSWDKTNNHSTTLGSGMFYN 300

Query: 301 EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTT 360
           EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPS T
Sbjct: 301 EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSIT 360

Query: 361 SPARVVPPPPMRVNVHKEVVVVPKRRPGSILGCKSEKAVRVAEGN-KYERKMGGNKSNNS 420
           SPARVVPPPPMRVNVHKEV VVPKRRP SILGC SEKAVRVAEGN KY+ KM GNKSNNS
Sbjct: 361 SPARVVPPPPMRVNVHKEVFVVPKRRPSSILGCNSEKAVRVAEGNNKYDHKMSGNKSNNS 420

Query: 421 DYLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLPLPLPLPHHGLATRSLPRPYS 480
           DYL MEQR+SESLMAVKRFEDETKVGG SFKSQGSSSMLPL  P     L TRSLPRPYS
Sbjct: 421 DYLHMEQRDSESLMAVKRFEDETKVGGLSFKSQGSSSMLPLAPPA--RALGTRSLPRPYS 480

Query: 481 PRLTNITFNMQ 489
           PRLTN+TFNMQ
Sbjct: 481 PRLTNMTFNMQ 489

BLAST of Chy5G097140 vs. NCBI nr
Match: XP_038889450.1 (protein PHYTOCHROME KINASE SUBSTRATE 1-like [Benincasa hispida])

HSP 1 Score: 655 bits (1691), Expect = 3.88e-232
Identity = 384/510 (75.29%), Postives = 419/510 (82.16%), Query Frame = 0

Query: 1   MDIFTSISSKTLPFDTHIDSNNN-LGVYGDTSFSSYLSAKEDHDFI-RKLAESTRYLKSP 60
           MDIF+SIS+KTLPFDTHID+NNN +GVYGD S     S KEDH FI  KL ESTRYLKS 
Sbjct: 1   MDIFSSISTKTLPFDTHIDNNNNNIGVYGDPS-----SYKEDHTFIIHKLTESTRYLKSS 60

Query: 61  NMIPCSRGGEDGEIGIFGAEKYFNGGMEDESTQRSSNNHPSSQKLDKLIV--AH-----M 120
           N IP S G EDGEIGIFGAEKYFNGG+E++ T+       S+QKLDKL+V  AH     +
Sbjct: 61  NTIPGSGGHEDGEIGIFGAEKYFNGGIENDGTRTER----SAQKLDKLLVLAAHQQHNLL 120

Query: 121 EEALKLPKPRLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIANNSYSLQKRSS-- 180
           EE+LKLPKPRLGTPSVGSESSS+NSQRPLL +VK+       +TTIANN+YSLQKRSS  
Sbjct: 121 EESLKLPKPRLGTPSVGSESSSINSQRPLLNVVKN-------STTIANNNYSLQKRSSSS 180

Query: 181 NNNKSFLSNTLGYCMCCTSNKKSTAVEDVGEISFSNAVTTNPTR-----NNINNILDRET 240
           N+NKSFLSNT GYCMCC+S++KS AVEDVGEISFSNAVTT PTR     NN NNI +RET
Sbjct: 181 NHNKSFLSNTFGYCMCCSSSEKS-AVEDVGEISFSNAVTTKPTRSNNNNNNNNNIFERET 240

Query: 241 PSFRGFPTAASS--LKMVHLQEPEEVVERKSLEVFGSPMTGRHRNNKPMSLEKRLTMLSW 300
           PSFRGFPTAASS  LKM+H QEPEEV ERKSLEVFGSP+ GR RNNKP+SLEKRL MLSW
Sbjct: 241 PSFRGFPTAASSSSLKMIHFQEPEEVGERKSLEVFGSPVMGRLRNNKPISLEKRLAMLSW 300

Query: 301 DKTNNHSTTLGSGMFYNEDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWS 360
           D TNN++ +LGSG+FYNEDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWS
Sbjct: 301 DHTNNNNNSLGSGVFYNEDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWS 360

Query: 361 VVTASALDFDERRPSTTSPARVVPPP-PMRVNVHKEVVVVPKRRPGSILGCKSEKAVRVA 420
           VVTASALDFDERR STTSP RVV P  PMRVNV+KEVV   KRRP SILGCKSEKAVRVA
Sbjct: 361 VVTASALDFDERRLSTTSPTRVVAPSLPMRVNVNKEVVQ--KRRPSSILGCKSEKAVRVA 420

Query: 421 E-GNKYERKMGGNKSNNSDYLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSS-SMLPL 480
           E  NKY RKM G KSN  DY      NSESL+AVKR EDETKVGG SF+SQGSS S+LPL
Sbjct: 421 EDNNKYGRKMNG-KSNYCDY------NSESLVAVKRSEDETKVGGLSFRSQGSSASLLPL 480

Query: 481 PLPLPHHGLATRSLPRPYSPRLTNITFNMQ 489
           P P PH  LATR LPRPYSPRLTNI+FN+Q
Sbjct: 481 PPP-PHRALATRPLPRPYSPRLTNISFNIQ 483

BLAST of Chy5G097140 vs. NCBI nr
Match: XP_022998592.1 (protein PHYTOCHROME KINASE SUBSTRATE 1-like [Cucurbita maxima])

HSP 1 Score: 355 bits (912), Expect = 1.58e-115
Identity = 237/431 (54.99%), Postives = 272/431 (63.11%), Query Frame = 0

Query: 70  GEIGIFGAEKYFNGGME---DESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKPRLGTPS 129
           GEIG+F AEKYFNGG++   D       NN PS   LD        +    P P L TPS
Sbjct: 16  GEIGVFRAEKYFNGGLDIHVDSPRINPPNNKPSKN-LD-------PDGPFTPNPWLATPS 75

Query: 130 VGSES--SSVNSQRPLLKIVKSTTTTSAIATTIANNSYSLQKRSSNNNKSFLSNTLGY-C 189
           V SES  ++ N+Q+PLL   K+                       +N K FLS+ LGY C
Sbjct: 76  VASESCTTTTNTQKPLLNTHKT-----------------------HNTKGFLSHALGYYC 135

Query: 190 MCCTSNKKSTAVEDVGEISFSNAVTTNPTRNNINNILDRETPSFR--GFPTAASSLKMVH 249
           MC + + K T  +D+GEISFS  VTT P            T SFR   F     SLK+VH
Sbjct: 136 MCYSRSDKPT--DDIGEISFSKPVTTAP------------TASFRDNSFTFQNGSLKLVH 195

Query: 250 LQEPEEVVERKSLEVFGSPMTGRHRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYNED 309
            QEPE  VERKSLEVFGSP+ GRHRN KP SLEK+L MLSWD TN+ S         N D
Sbjct: 196 FQEPE--VERKSLEVFGSPVIGRHRN-KPFSLEKKLAMLSWDHTNSSSNN-------NND 255

Query: 310 EVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTTSP 369
           E NSDCSSDLFEIESLTKQTNPF SPT S YAPSEASV+WSVVTASALDFDERR STTSP
Sbjct: 256 EANSDCSSDLFEIESLTKQTNPFQSPTPSYYAPSEASVEWSVVTASALDFDERRISTTSP 315

Query: 370 ARVVPPPP-MRVNVHKEV-VVVPKRRPGSILGCKSEKAVRVAEGNKYERKMGGNKSNNSD 429
           ARV PPPP  RV V+++V VVV KRR GS+LGCKSEKAVRVAE N   RK+ G  + NS 
Sbjct: 316 ARVAPPPPPRRVGVNEQVAVVVQKRRSGSLLGCKSEKAVRVAEDNSNGRKISGKVNFNSS 375

Query: 430 YLLMEQRNSESL-MAVKRFEDETKVGGFSFKSQGSSSMLPLPLPLPHHGLATRSLPRPYS 489
              +      S+ ++V R EDET+V  FSF+SQ    +LP      HH LATRSLPRPYS
Sbjct: 376 SSSVSVSVPVSVPVSVNRLEDETRVRSFSFRSQAPQPLLPSS----HHALATRSLPRPYS 387

BLAST of Chy5G097140 vs. NCBI nr
Match: XP_023523597.1 (protein PHYTOCHROME KINASE SUBSTRATE 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 347 bits (889), Expect = 3.78e-112
Identity = 233/430 (54.19%), Postives = 265/430 (61.63%), Query Frame = 0

Query: 70  GEIGIFGAEKYFNGGME---DESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKPRLGTPS 129
           GEIG+F AEKYFNGG++   D       NN PS       I    +E    P P L TPS
Sbjct: 16  GEIGVFRAEKYFNGGLDIHVDSPRTNPPNNKPS-------INLAPDEPFT-PTPWLATPS 75

Query: 130 VGSES----SSVNSQRPLLKIVKSTTTTSAIATTIANNSYSLQKRSSNNNKSFLSNTLGY 189
           V SES    ++  +Q+PLL   K+                       +N K FLS+ LGY
Sbjct: 76  VASESCTTTTTTTTQKPLLNTHKT-----------------------HNTKGFLSHALGY 135

Query: 190 -CMCCTSNKKSTAVEDVGEISFSNAVTTNPTRNNINNILDRETPSFR--GFPTAASSLKM 249
            CMC + + K +  +D+GEISFS  VTT P            T SFR   F     SLK+
Sbjct: 136 YCMCYSRSDKPS--DDIGEISFSRPVTTAP------------TASFRDNSFTFQNGSLKL 195

Query: 250 VHLQEPEEVVERKSLEVFGSPMTGRHRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYN 309
           VH QEPE  VERKSLEVFGSP+ GRHRN KP SLEK+L MLSWD TNN           N
Sbjct: 196 VHFQEPE--VERKSLEVFGSPVIGRHRN-KPFSLEKKLAMLSWDHTNN-----------N 255

Query: 310 EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTT 369
            DE NSDCSSDLFEIESLTKQTNPF SPT S YAPSEASV+WSVVTASALDFDERR STT
Sbjct: 256 NDEANSDCSSDLFEIESLTKQTNPFQSPTPSYYAPSEASVEWSVVTASALDFDERRISTT 315

Query: 370 SPARVVPPPPMRVNVHKEV-VVVPKRRPGSILGCKSEKAVRVAEGNKYERKMGGNKSNNS 429
           SPARV  PPP RV V++ V VVV KRR GS+LGCKSEKAVRVAE N   RK+ G  + NS
Sbjct: 316 SPARVAAPPPRRVGVNEPVAVVVQKRRSGSLLGCKSEKAVRVAEDNSNGRKINGKVNFNS 375

Query: 430 DYLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLPLPLPLPHHGLATRSLPRPYS 488
                   +S   ++V RFEDET+V  FSF+SQ     L   LP   H LATRSLPRPYS
Sbjct: 376 S-------SSSVSVSVNRFEDETRVRSFSFRSQAPPPSL---LPSSRHALATRSLPRPYS 376

BLAST of Chy5G097140 vs. TAIR 10
Match: AT2G02950.1 (phytochrome kinase substrate 1 )

HSP 1 Score: 142.9 bits (359), Expect = 6.8e-34
Identity = 155/444 (34.91%), Postives = 217/444 (48.87%), Query Frame = 0

Query: 5   TSISSKTLPFDTHIDSNNNLGVYGDTSFSSYLSAKEDH-DFIRKLAESTRYLK------- 64
           +S S+    FD   ++N++  +Y   S SSYLS+KED     +KL E ++ L        
Sbjct: 7   SSASTPKTSFDFMKNNNSHSSLY--VSSSSYLSSKEDALVTTKKLMEPSKTLNMSINPKQ 66

Query: 65  ---SPNMIPCSRGGEDGEIGIFGAEKYFNGGMEDESTQRSSNNHPSSQKLDKLIVAHMEE 124
                      +  ED EIG+FGAEKYFNG M  +S Q SS    ++ ++++ +V   + 
Sbjct: 67  EEFGDEKKMVKKAPEDPEIGVFGAEKYFNGDM--DSDQGSSVLSLTNPEVERTVVDSKQS 126

Query: 125 ALKLPKPRLGTPSVGSESSSVNSQRPLL--KIVKSTTTTSAIATTIANNSYSLQKRSSNN 184
           A    K   GTPSV SE SS NSQ  LL  K+V S  +    +     NS    ++ +NN
Sbjct: 127 A----KKSTGTPSVRSE-SSWNSQSVLLQNKLVNSCNS----SFKEKKNSNGQIQKVTNN 186

Query: 185 NKSFLSNTLGYCMCCTSNKKSTAVEDVGEISFSNAVTTNPTRNNINNILDRETPSFRGFP 244
            KSFL+N LG C C  S+  S  VE+   +  S     +P   NI+ I  R         
Sbjct: 187 KKSFLAN-LG-CKCACSDGDSVDVEEKTSVKRS----ADP---NISVITMRS-------- 246

Query: 245 TAASSLKMVHLQEPEEVVERKSLEVFGSPMTGRHRNNKPMSLEKRLTMLSWDKTNNHSTT 304
           +A  + +++ +Q+ EE+ +RKSLEVFGSP+       K   ++K+L +  W        T
Sbjct: 247 SADMNTELIKIQKQEELSQRKSLEVFGSPVA---IEKKSSVVQKKLPLPPWKSRTEEDDT 306

Query: 305 LGSGMFYNEDEVNSDCSSDLFEIESLTKQTNPF--------HSPTASCYAPSEASVDWSV 364
              G         SD SSDLFEIE LT    PF         SPT  CYAPSE SV+WS+
Sbjct: 307 KSEG---------SDSSSDLFEIEGLTGNPKPFLTRQGSDPASPT--CYAPSEVSVEWSI 366

Query: 365 VTASALDFDERRPSTTSPARVVPPPPM-RVNVHKEVVVVPKRRP--------GSILGCKS 419
           VTASA DF       TSP R   P  + R+ +  +    P+RR         G ++ CKS
Sbjct: 367 VTASAADFSVMSECATSPVRRNRPTQIPRIPITAK--SAPQRRKSSSSSGGNGFLMSCKS 402

BLAST of Chy5G097140 vs. TAIR 10
Match: AT1G14280.1 (phytochrome kinase substrate 2 )

HSP 1 Score: 130.2 bits (326), Expect = 4.6e-30
Identity = 149/437 (34.10%), Postives = 212/437 (48.51%), Query Frame = 0

Query: 5   TSISSKTLPFDTHIDSNNNL-GVYGDTSFS----SYLSAKEDHDFIRKLAES-TRYLKSP 64
           +S S+  + FD  +++NNN   +YG  S S    SYL++KED    + L    T  +   
Sbjct: 6   SSSSTPNVSFDFMMNNNNNSNNLYGPFSSSSTSFSYLTSKEDALTQKNLMSGITNDVLGI 65

Query: 65  NMIPCSRGGEDGEIGIFGAEKYFNGGMEDESTQRSSNNHPSSQ-KLDKLIVAHMEEALKL 124
           N     +  ED EI +FGAEKYFNG M+ + + R  +  P  +  ++++ V   + +   
Sbjct: 66  N----KKASEDLEISVFGAEKYFNGDMDSDHSPRLVSPLPDPEVPIERIFVGPKQSS--- 125

Query: 125 PKPRLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIANNSYSLQKRSSNNN----- 184
            K    TPS+ SE SS NSQ  LL+  K       I    + NSY  +K  S+N+     
Sbjct: 126 -KNSSETPSLRSE-SSWNSQSLLLQ-SKYVEKKKNIKKNSSCNSYFQEKDMSSNHKVSNK 185

Query: 185 KSFLSNTLGYCMCCTSNKKST-AVEDVGEISFSNAVTTNPTRNNINNILDRETPSFRGFP 244
           KSFL+ TLG C C  SN  S   V+D    S    + T                    F 
Sbjct: 186 KSFLA-TLG-CRCVCSNWSSVDVVDDKRRSSGLKKIKTQ-----------------LSFS 245

Query: 245 TAASSLKMVHLQEPEEVVE-RKSLEVFGSPMTGRHRNNKPMSLEKRLTM--LSWDKTNN- 304
              SS   +H Q+ E ++E RKSLE+FGSP+           +EKR+      W+ +++ 
Sbjct: 246 GDLSSEMKIHQQQQEAMLEQRKSLEIFGSPL-----------IEKRIIQKKFPWEYSSSA 305

Query: 305 HSTTLGSGMFYNEDEVN--SDCSSDLFEIESLTKQTNPFHSPTASC-------YAPSEAS 364
                G  + Y E+E    SD S+DLFEIESLT + NPF +   S        YAPSE S
Sbjct: 306 KKEEHGFSVKYEEEEDGSVSDVSTDLFEIESLTGKANPFLARQGSSDPDSPDGYAPSEVS 365

Query: 365 VDWSVVTASALDFDERRPSTTSPAR-----VVPPPPMRVNVHKEVVVVPKRR----PGSI 407
           + WSVVTAS  DF       TSP +      +P  P+    ++E  + P+RR     G +
Sbjct: 366 IQWSVVTASVADFSVMSECATSPVKKNRSFQIPRIPIMAKSNRE--IAPQRRKSSSSGLL 399

BLAST of Chy5G097140 vs. TAIR 10
Match: AT1G18810.1 (phytochrome kinase substrate-related )

HSP 1 Score: 71.2 bits (173), Expect = 2.5e-12
Identity = 107/376 (28.46%), Postives = 149/376 (39.63%), Query Frame = 0

Query: 65  RGGEDGEIGIFGAEKYFNGGMED-ESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKP--- 124
           +   D EIG+FGAEKYF+  ++  +ST   +  H      D             P P   
Sbjct: 41  KADNDAEIGVFGAEKYFSMKLDHVDSTADITKQHEKENTHDHPNANPNPNPNPHPHPHPQ 100

Query: 125 ------------RLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIANNSYSLQKRS 184
                       R GTPSV SE SS NSQ  L++I               NN    ++R 
Sbjct: 101 LTKTTSSRSKTSRHGTPSVRSE-SSCNSQTFLMRI---------------NNHNENKQRK 160

Query: 185 SNNNKSFLSNTLGYCMCCTSNKKSTAVEDVGEISFSNAVTTNPTRNNINNILDRETPSFR 244
            N+     S + G   C             G  S    V T+P  +  +   DR+  ++ 
Sbjct: 161 MNDT----SISFGGFRC------------YGPCSGVKTVHTDPKNSCKSRNSDRDFVAYD 220

Query: 245 GFPTAASSLKMVHLQEPEEVVERKSLEVFGSPMTGR--HRNNKPMSLEKRLTMLSWD--- 304
                       H  +P    E K  +            R++  M+LE++L++L+WD   
Sbjct: 221 A---------RKHNDKPRLHFEAKKADFHAQEKISLPIQRSDIAMNLERKLSLLTWDAIP 280

Query: 305 -----KTNNHSTT-LGSGMFYN--EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPS 364
                K NNH+     S M  N  E+E  S  SSDLFEIE++          T+S Y PS
Sbjct: 281 NQLSTKNNNHNNNGNNSSMSSNTQEEETASVASSDLFEIENI----------TSSVYEPS 340

Query: 365 EASVDWSVVTASAL------DFDERRPSTTSPARVVPPPPMRVNVHKEVVVVPKRRPGSI 406
           EAS+ WSVVT S        DFD  +  T +   V   P         V+    R  G +
Sbjct: 341 EASIGWSVVTGSMADQSVISDFDMMKRVTRNGPVVKTKP---------VIGEKVRSAGFL 356

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SWI19.6e-3334.91Protein PHYTOCHROME KINASE SUBSTRATE 1 OS=Arabidopsis thaliana OX=3702 GN=PKS1 P... [more]
Q9M9T46.5e-2934.10Protein PHYTOCHROME KINASE SUBSTRATE 2 OS=Arabidopsis thaliana OX=3702 GN=PKS2 P... [more]
Q8GXS83.6e-1128.46Protein PHYTOCHROME KINASE SUBSTRATE 3 OS=Arabidopsis thaliana OX=3702 GN=PKS3 P... [more]
Match NameE-valueIdentityDescription
A0A0A0LKV24.8e-26998.16Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G108600 PE=4 SV=1[more]
A0A5A7SNC21.0e-25093.28Protein PHYTOCHROME KINASE SUBSTRATE 1 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3B4P01.0e-25093.28protein PHYTOCHROME KINASE SUBSTRATE 1 OS=Cucumis melo OX=3656 GN=LOC103485779 P... [more]
A0A6J1K8F01.6e-9454.78protein PHYTOCHROME KINASE SUBSTRATE 1-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1GCF62.1e-9153.74protein PHYTOCHROME KINASE SUBSTRATE 1-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
Match NameE-valueIdentityDescription
XP_004138989.10.098.16protein PHYTOCHROME KINASE SUBSTRATE 1 [Cucumis sativus] >KGN61382.1 hypothetica... [more]
XP_008441702.10.093.28PREDICTED: protein PHYTOCHROME KINASE SUBSTRATE 1 [Cucumis melo] >KAA0032290.1 p... [more]
XP_038889450.13.88e-23275.29protein PHYTOCHROME KINASE SUBSTRATE 1-like [Benincasa hispida][more]
XP_022998592.11.58e-11554.99protein PHYTOCHROME KINASE SUBSTRATE 1-like [Cucurbita maxima][more]
XP_023523597.13.78e-11254.19protein PHYTOCHROME KINASE SUBSTRATE 1-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT2G02950.16.8e-3434.91phytochrome kinase substrate 1 [more]
AT1G14280.14.6e-3034.10phytochrome kinase substrate 2 [more]
AT1G18810.12.5e-1228.46phytochrome kinase substrate-related [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR039615Protein PHYTOCHROME KINASE SUBSTRATEPANTHERPTHR33781PROTEIN PHYTOCHROME KINASE SUBSTRATE 1-RELATEDcoord: 3..447
NoneNo IPR availablePANTHERPTHR33781:SF4PROTEIN PHYTOCHROME KINASE SUBSTRATE 1coord: 3..447

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy5G097140.1Chy5G097140.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
biological_process GO:0009638 phototropism
molecular_function GO:0016301 kinase activity