Chy5G095970 (gene) Cucumber (hystrix) v1

Overview
NameChy5G095970
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptioncytochrome b5-like
LocationchrH05: 5995328 .. 5998123 (-)
RNA-Seq ExpressionChy5G095970
SyntenyChy5G095970
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTGGAGATGGAAAGCTCTTTACATTGGCTGAAGTCTCTCAGCACAACAATCCCAAGGACTGTTGGTTGATCATCAATGGCAAGGTTTTTCTTCATACCCTTTTCTTTGTTTTTGTTTTTGGGATTTCTTCTTTCTATTATGCCTGCAGGAGTTTGATTCTCTGAAGTTGTTTGTTCTTGTTTGCTCTTGAAATCTCGCATGCATTCTGGACAGACCCATTATTTTTCACTGTTGAATCAATATGTTTATCCATGGCTGCAAGCTGCTGCATGGTGAGCTTTGCCGATGATGTTGTATTGGGCTGGCTAATGAAGGATTTTAGTTGCACAGTACAGTGTAAATTGAACTATTCCTTTGCCTTTTTTCTTGGGAATGCAAAGAGATTCAGGCTTCATTTTGTAGCTTTTAAATCATGGCAGCAAACGGTGCTTGAATACGAGGGAAATTTGTGTTTGGTGTATTTAAAACTTAAACGTGAAAAGTAGGATAAAATTTGAGTTTTCTGTTGCTGGTTAGAAGGCATCATATCCTTTGGCATTATGATCAGGTTCACCAAACATTAATTACAGTTAGCATTCCCTCTATTCAGTCACTTTTATCGTTGGCAAAATCAATTTCAAGCGCAGCCTCAATCGTTGAAGAGTGATGTTTCATCCTCACCGGGGCTTAGAGGATTGATTTTTGGTTGATTTCATAAGTTTTGGAAAGGAATGGGTTTTATAAATGTGTATTCATATCACGTATATTTGGTTTAGGAGTTTGTGATCTATAAAATATCATAAAGTCGATTTTATAAGCCTGAGAATGTGTAACATTGGTTAGTGTAGTTCATGAGGTTAACAAACAAATTTGCATACGAAGATAAACAAACTCACATGATGGCAAGCATAGATTTGGCTTCTTAATTTCGGAATATACATTTCTTGCTCTGCTGTGCTCACCTTACCAAGTATAGAAGACTGAACTGGAGATTTATGATTTATAGATCTTAAAAAATGCGCTGTGTTTAGTGTCTTGAAGGAACTTATTTTGTGTCTTTGATTCCTTTTAGGACCAGGATCTCATTGTATGGATAGAATCTAAGCCTTCTTCATGTTTGGTAGTCCTGTTATGAAACAAATGCTTTCATATTTTTGCCAATTCACTGACAGGAATATCAGTGTAGAAAAACTTTGTTTCAAGAGCGGTTAAATATGAAGATCACTGACTTGAAAAATGAACTGATCCAAAGATCTCATTTCATTTTTGTGTGTGTGAATGCTCTTATCTACTTGAGGGAACTCTTATGCATATACTATGTGAGAACTGGACTCCAGTGGGGTTGGACATCCAACACTTCGTTCAAACCTAACCAATAAAAAAACCAGTCGTCTGGGGGATGAATAGTCAACATACATCGTTGTTATGCTGTCTGGTGGGTCAATTTCTGTCGCTGCAGACCTCATCTGCCATAAAAAATTTCTTTCATTCTGATTCTTCTGCATCCAATTGATGACTTTTTTCTTCGGCATCCATAATATGATCTCAAAGCAATATCCTGAAGCCTCAGTCTGTTGAAATTGCACATTCTATTGATGTTAAAAATTTATTTCCAACCATCAGACTTACGTCCGGCCCTACTAATCTTCAAGAAGGCTTCATCCACGCATTGGAAAGATTCATGACATACGAAGAGTTTTAAAATATTGACTTCCCCTACCTCGGCTTGAAAGTTGAACCCAGATATTTCACGTAGTTCTCTAGATTTAAAGTCCTTTACACTAAAGCCATCCTTTGGGTAAAATCAGAAATGTAGTGTCCAATTAGTAGTGGCAAGCTCTTGCTGGCTGTACTTGTTCATCATTGTGCTATAAACTCTGTTTTCGTCCTTATCAATGTAATGTGAAATCTCTTTCTTGACCTACCATATCAATAAAGGATAAGGATACTCTAGGAATGCTAATTGTGTTGGAGAATGCCAAGTACTAGTTTCTCAACAAACAAACTTGATAACCAAGACCATTAGCCAACTCAAGTTTCTCAAGGTTCTAAGTGTAAAGTTAGAAGCAGGTGGATAACATGAAAATGCATGAAACGTCCCTCAACTTTGCCCGATTCCTACTCGGAATTGAATATAAATTATATCTTCTCAAAGAACAACACTTGTGCATTTTTACTATTGTTTTGCTTCTAAAATTTCTTGAATTATAGTAATAGAAAGTCGAACAATTTTGATTTTCTCACTTGTACTAGGAGCTCAGAATGCGATCAACAAGCTTTCAGTTGTTTTGGCAGTTGGCCTGGAATTAAACTAAATGAATCGTGCATAGATTTTATCTTTGATGGGTATGGTTTTTAGCTTGAGGTTACCATTTTGTCCATCTTTGATTGTCTCAACAGGTTTACAATGTAACCGAGTTCTTGGAAGACCATCCCGGTGGTGACGAGGTGTTGTTGTCTGGCACAGGTAATAAGTCCTCCAGTCATACAGCATTAGTTATTTACAATGTACATTGAGTGTTTGTGCATCGTTTACACACTATGTAATTTTTATTACCACAGGTAAGGATGCAACTGATGATTTTGAAGACGTGGGTCATAGTGAGAGCGCAAGAGAAATGATGAGCAAATACTACGTTGGAGAGATCGATATCTCGACAATCCCAGAACGGTTGACATACACAGGTCCAAAGCAACCTCATTACAATCAGGATAAGACAACCGAGTTCATTATTAAGCTCCTCCAGTTCATGGTTCCTCTCGCAATCTTGGGATTGGCGGTTGGTATCCGCTTTTACAACAAATCAAGCTGA

mRNA sequence

ATGGGTGGAGATGGAAAGCTCTTTACATTGGCTGAAGTCTCTCAGCACAACAATCCCAAGGACTGTTGGTTGATCATCAATGGCAAGGTTTACAATGTAACCGAGTTCTTGGAAGACCATCCCGGTGGTGACGAGGTGTTGTTGTCTGGCACAGGTAAGGATGCAACTGATGATTTTGAAGACGTGGGTCATAGTGAGAGCGCAAGAGAAATGATGAGCAAATACTACGTTGGAGAGATCGATATCTCGACAATCCCAGAACGGTTGACATACACAGGTCCAAAGCAACCTCATTACAATCAGGATAAGACAACCGAGTTCATTATTAAGCTCCTCCAGTTCATGGTTCCTCTCGCAATCTTGGGATTGGCGGTTGGTATCCGCTTTTACAACAAATCAAGCTGA

Coding sequence (CDS)

ATGGGTGGAGATGGAAAGCTCTTTACATTGGCTGAAGTCTCTCAGCACAACAATCCCAAGGACTGTTGGTTGATCATCAATGGCAAGGTTTACAATGTAACCGAGTTCTTGGAAGACCATCCCGGTGGTGACGAGGTGTTGTTGTCTGGCACAGGTAAGGATGCAACTGATGATTTTGAAGACGTGGGTCATAGTGAGAGCGCAAGAGAAATGATGAGCAAATACTACGTTGGAGAGATCGATATCTCGACAATCCCAGAACGGTTGACATACACAGGTCCAAAGCAACCTCATTACAATCAGGATAAGACAACCGAGTTCATTATTAAGCTCCTCCAGTTCATGGTTCCTCTCGCAATCTTGGGATTGGCGGTTGGTATCCGCTTTTACAACAAATCAAGCTGA

Protein sequence

MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERLTYTGPKQPHYNQDKTTEFIIKLLQFMVPLAILGLAVGIRFYNKSS*
Homology
BLAST of Chy5G095970 vs. ExPASy Swiss-Prot
Match: O48845 (Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 1.1e-60
Identity = 106/133 (79.70%), Postives = 121/133 (90.98%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MG + K+FTL+EVS+HN   DCW++INGKVYNVT+FLEDHPGGD+VLLS TGKDATDDFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  DVGHSESAREMMSKYYVGEIDISTIPERLTYTGPKQPHYNQDKTTEFIIKLLQFMVPLAI 120
           DVGHSESAREMM +YYVGEID +TIP+++ YT PKQPHYNQDKT+EFIIKLLQF+VPLAI
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYNKS 134
           LGLAVGIR Y KS
Sbjct: 121 LGLAVGIRIYTKS 133

BLAST of Chy5G095970 vs. ExPASy Swiss-Prot
Match: P49099 (Cytochrome b5, seed isoform OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 6.3e-59
Identity = 103/134 (76.87%), Postives = 116/134 (86.57%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGG  K+FTLAEVS HNN KDCWLII+GKVYNVT+FLEDHPGG EVLLS TGKDATDDFE
Sbjct: 1   MGGQSKVFTLAEVSNHNNAKDCWLIISGKVYNVTKFLEDHPGGGEVLLSATGKDATDDFE 60

Query: 61  DVGHSESAREMMSKYYVGEIDISTIPERLTYTGPKQPHYNQDKTTEFIIKLLQFMVPLAI 120
           D+GHS SAR M+ +YYVG+ID STIP ++ YT PKQPHYNQDKTTEFI+KLLQF+VPL I
Sbjct: 61  DIGHSSSARAMLDEYYVGDIDSSTIPTKVKYTPPKQPHYNQDKTTEFIVKLLQFLVPLII 120

Query: 121 LGLAVGIRFYNKSS 135
           LG+A G+ FY K S
Sbjct: 121 LGVAFGVHFYTKQS 134

BLAST of Chy5G095970 vs. ExPASy Swiss-Prot
Match: P49098 (Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 2.4e-58
Identity = 101/134 (75.37%), Postives = 118/134 (88.06%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGG+ K+FTLAEVSQHNN KDCWL+I+GKVY+VT+FL+DHPGGDEVLLS TGKDATDDFE
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFE 60

Query: 61  DVGHSESAREMMSKYYVGEIDISTIPERLTYTGPKQPHYNQDKTTEFIIKLLQFMVPLAI 120
           DVGHS SAR M+ +YYVG+ID +TIP +  YT P QPHYNQDKT+EF++KLLQF+VPL I
Sbjct: 61  DVGHSSSARAMLDEYYVGDIDSATIPTKTKYTPPNQPHYNQDKTSEFVVKLLQFLVPLII 120

Query: 121 LGLAVGIRFYNKSS 135
           LG+A GIRFY K S
Sbjct: 121 LGVAFGIRFYTKQS 134

BLAST of Chy5G095970 vs. ExPASy Swiss-Prot
Match: O04354 (Cytochrome b5 OS=Borago officinalis OX=13363 PE=2 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 1.9e-55
Identity = 98/130 (75.38%), Postives = 114/130 (87.69%), Query Frame = 0

Query: 5   GKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFEDVGH 64
           GK+FTLAEV+QHNN KDCWLIINGKVY+VT+FLEDHPGGD+VLLS TGKDATDDFED+GH
Sbjct: 2   GKIFTLAEVAQHNNSKDCWLIINGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFEDIGH 61

Query: 65  SESAREMMSKYYVGEIDISTIPERLTYTGPKQPHYNQDKTTEFIIKLLQFMVPLAILGLA 124
           S SA+ M+ +YYVG+ID S+IP ++ YT PKQP YN DKT EF+IKLLQF+VPL IL  A
Sbjct: 62  SSSAKAMLDEYYVGDIDSSSIPSQVKYTPPKQPLYNPDKTREFVIKLLQFLVPLVILAGA 121

Query: 125 VGIRFYNKSS 135
           +GIRFY KSS
Sbjct: 122 IGIRFYTKSS 131

BLAST of Chy5G095970 vs. ExPASy Swiss-Prot
Match: P49100 (Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 211.8 bits (538), Expect = 4.7e-54
Identity = 97/128 (75.78%), Postives = 110/128 (85.94%), Query Frame = 0

Query: 6   KLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFEDVGHS 65
           K++TL EV++HN+  DCWLII GKVYNV++FLEDHPGGD+VLLS TGKDATDDFEDVGHS
Sbjct: 7   KVYTLEEVAKHNSKDDCWLIIGGKVYNVSKFLEDHPGGDDVLLSSTGKDATDDFEDVGHS 66

Query: 66  ESAREMMSKYYVGEIDISTIPERLTYTGPKQPHYNQDKTTEFIIKLLQFMVPLAILGLAV 125
            +AR MM +YYVG+ID STIP R  Y  PKQPHYNQDKT EFIIK+LQF+VPLAILGLAV
Sbjct: 67  TTARAMMDEYYVGDIDTSTIPARTKYVPPKQPHYNQDKTPEFIIKILQFLVPLAILGLAV 126

Query: 126 GIRFYNKS 134
            IR Y KS
Sbjct: 127 AIRIYTKS 134

BLAST of Chy5G095970 vs. ExPASy TrEMBL
Match: A0A5D3CSF5 (Cytochrome b5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold472G00160 PE=3 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 2.0e-71
Identity = 130/134 (97.01%), Postives = 134/134 (100.00%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGGDGKL+TLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 1   MGGDGKLYTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60

Query: 61  DVGHSESAREMMSKYYVGEIDISTIPERLTYTGPKQPHYNQDKTTEFIIKLLQFMVPLAI 120
           DVGHSESAREMMSKYY+GEIDISTIPE+LTYTGPKQPHYNQDKTTEFIIKLLQF+VPLAI
Sbjct: 61  DVGHSESAREMMSKYYIGEIDISTIPEQLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYNKSS 135
           LGLAVGIRFYNKSS
Sbjct: 121 LGLAVGIRFYNKSS 134

BLAST of Chy5G095970 vs. ExPASy TrEMBL
Match: A0A1S3C5R9 (cytochrome b5-like OS=Cucumis melo OX=3656 GN=LOC103496974 PE=3 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 2.0e-71
Identity = 130/134 (97.01%), Postives = 134/134 (100.00%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGGDGKL+TLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 1   MGGDGKLYTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60

Query: 61  DVGHSESAREMMSKYYVGEIDISTIPERLTYTGPKQPHYNQDKTTEFIIKLLQFMVPLAI 120
           DVGHSESAREMMSKYY+GEIDISTIPE+LTYTGPKQPHYNQDKTTEFIIKLLQF+VPLAI
Sbjct: 61  DVGHSESAREMMSKYYIGEIDISTIPEQLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYNKSS 135
           LGLAVGIRFYNKSS
Sbjct: 121 LGLAVGIRFYNKSS 134

BLAST of Chy5G095970 vs. ExPASy TrEMBL
Match: A0A0A0LHS9 (Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G138200 PE=3 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 5.7e-71
Identity = 130/134 (97.01%), Postives = 132/134 (98.51%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGGDGKLFT AEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 1   MGGDGKLFTFAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60

Query: 61  DVGHSESAREMMSKYYVGEIDISTIPERLTYTGPKQPHYNQDKTTEFIIKLLQFMVPLAI 120
           DVGHSESARE MSKYYVGEIDIST+PERLTYTGPKQPHYNQDKTTEFIIKLLQF+VPLAI
Sbjct: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYNKSS 135
           LGLAVGIRFYNKSS
Sbjct: 121 LGLAVGIRFYNKSS 134

BLAST of Chy5G095970 vs. ExPASy TrEMBL
Match: A0A6J1CAX1 (cytochrome b5-like OS=Momordica charantia OX=3673 GN=LOC111009917 PE=3 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 3.2e-66
Identity = 120/134 (89.55%), Postives = 129/134 (96.27%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGGDGK+FTLAEVSQHN PKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 50  MGGDGKIFTLAEVSQHNTPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 109

Query: 61  DVGHSESAREMMSKYYVGEIDISTIPERLTYTGPKQPHYNQDKTTEFIIKLLQFMVPLAI 120
           DVGHS+SARE M+KYYVGEIDISTIPE++TY+  KQPHYNQDKTTEFI+KLLQF+VPLAI
Sbjct: 110 DVGHSDSARETMNKYYVGEIDISTIPEKMTYSATKQPHYNQDKTTEFIVKLLQFLVPLAI 169

Query: 121 LGLAVGIRFYNKSS 135
           LGLAVGIRFY KS+
Sbjct: 170 LGLAVGIRFYTKST 183

BLAST of Chy5G095970 vs. ExPASy TrEMBL
Match: A0A6J1KXV9 (cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111499200 PE=3 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 2.7e-65
Identity = 121/134 (90.30%), Postives = 128/134 (95.52%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGG GK+FTLAEVSQHN  +DCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 1   MGGHGKVFTLAEVSQHNTRQDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60

Query: 61  DVGHSESAREMMSKYYVGEIDISTIPERLTYTGPKQPHYNQDKTTEFIIKLLQFMVPLAI 120
           DVGHSESARE M+KYYVGEIDISTIPERLTYT  KQPHYNQDKTTEFI+KLLQF+VPLAI
Sbjct: 61  DVGHSESARESMNKYYVGEIDISTIPERLTYTASKQPHYNQDKTTEFIVKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYNKSS 135
           LGLAVGIRFY+KS+
Sbjct: 121 LGLAVGIRFYSKST 134

BLAST of Chy5G095970 vs. NCBI nr
Match: XP_008457249.1 (PREDICTED: cytochrome b5-like [Cucumis melo] >KAA0033284.1 cytochrome b5-like [Cucumis melo var. makuwa] >TYK14823.1 cytochrome b5-like [Cucumis melo var. makuwa])

HSP 1 Score: 276 bits (706), Expect = 1.16e-93
Identity = 130/134 (97.01%), Postives = 134/134 (100.00%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGGDGKL+TLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 1   MGGDGKLYTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60

Query: 61  DVGHSESAREMMSKYYVGEIDISTIPERLTYTGPKQPHYNQDKTTEFIIKLLQFMVPLAI 120
           DVGHSESAREMMSKYY+GEIDISTIPE+LTYTGPKQPHYNQDKTTEFIIKLLQF+VPLAI
Sbjct: 61  DVGHSESAREMMSKYYIGEIDISTIPEQLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYNKSS 134
           LGLAVGIRFYNKSS
Sbjct: 121 LGLAVGIRFYNKSS 134

BLAST of Chy5G095970 vs. NCBI nr
Match: XP_004138957.1 (cytochrome b5 [Cucumis sativus] >KGN61465.1 hypothetical protein Csa_005989 [Cucumis sativus])

HSP 1 Score: 275 bits (702), Expect = 4.71e-93
Identity = 130/134 (97.01%), Postives = 132/134 (98.51%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGGDGKLFT AEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 1   MGGDGKLFTFAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60

Query: 61  DVGHSESAREMMSKYYVGEIDISTIPERLTYTGPKQPHYNQDKTTEFIIKLLQFMVPLAI 120
           DVGHSESARE MSKYYVGEIDIST+PERLTYTGPKQPHYNQDKTTEFIIKLLQF+VPLAI
Sbjct: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYNKSS 134
           LGLAVGIRFYNKSS
Sbjct: 121 LGLAVGIRFYNKSS 134

BLAST of Chy5G095970 vs. NCBI nr
Match: XP_022138846.1 (cytochrome b5-like [Momordica charantia])

HSP 1 Score: 259 bits (661), Expect = 4.44e-86
Identity = 120/134 (89.55%), Postives = 129/134 (96.27%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGGDGK+FTLAEVSQHN PKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 50  MGGDGKIFTLAEVSQHNTPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 109

Query: 61  DVGHSESAREMMSKYYVGEIDISTIPERLTYTGPKQPHYNQDKTTEFIIKLLQFMVPLAI 120
           DVGHS+SARE M+KYYVGEIDISTIPE++TY+  KQPHYNQDKTTEFI+KLLQF+VPLAI
Sbjct: 110 DVGHSDSARETMNKYYVGEIDISTIPEKMTYSATKQPHYNQDKTTEFIVKLLQFLVPLAI 169

Query: 121 LGLAVGIRFYNKSS 134
           LGLAVGIRFY KS+
Sbjct: 170 LGLAVGIRFYTKST 183

BLAST of Chy5G095970 vs. NCBI nr
Match: XP_023006481.1 (cytochrome b5-like [Cucurbita maxima])

HSP 1 Score: 256 bits (653), Expect = 1.40e-85
Identity = 121/134 (90.30%), Postives = 128/134 (95.52%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGG GK+FTLAEVSQHN  +DCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 1   MGGHGKVFTLAEVSQHNTRQDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60

Query: 61  DVGHSESAREMMSKYYVGEIDISTIPERLTYTGPKQPHYNQDKTTEFIIKLLQFMVPLAI 120
           DVGHSESARE M+KYYVGEIDISTIPERLTYT  KQPHYNQDKTTEFI+KLLQF+VPLAI
Sbjct: 61  DVGHSESARESMNKYYVGEIDISTIPERLTYTASKQPHYNQDKTTEFIVKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYNKSS 134
           LGLAVGIRFY+KS+
Sbjct: 121 LGLAVGIRFYSKST 134

BLAST of Chy5G095970 vs. NCBI nr
Match: XP_038906915.1 (cytochrome b5-like [Benincasa hispida])

HSP 1 Score: 255 bits (651), Expect = 2.83e-85
Identity = 124/134 (92.54%), Postives = 127/134 (94.78%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           M GD KLFTLAEVSQHNN KDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 1   MDGDRKLFTLAEVSQHNNRKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60

Query: 61  DVGHSESAREMMSKYYVGEIDISTIPERLTYTGPKQPHYNQDKTTEFIIKLLQFMVPLAI 120
           DVGHSESAREMMSKY VGEIDISTIPER TYT  KQPHYNQDKTTEFIIKLLQF+VPLAI
Sbjct: 61  DVGHSESAREMMSKYSVGEIDISTIPERSTYTTSKQPHYNQDKTTEFIIKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYNKSS 134
           LGLAVGIRFY+KS+
Sbjct: 121 LGLAVGIRFYSKST 134

BLAST of Chy5G095970 vs. TAIR 10
Match: AT2G32720.1 (cytochrome B5 isoform B )

HSP 1 Score: 233.8 bits (595), Expect = 8.1e-62
Identity = 106/133 (79.70%), Postives = 121/133 (90.98%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MG + K+FTL+EVS+HN   DCW++INGKVYNVT+FLEDHPGGD+VLLS TGKDATDDFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  DVGHSESAREMMSKYYVGEIDISTIPERLTYTGPKQPHYNQDKTTEFIIKLLQFMVPLAI 120
           DVGHSESAREMM +YYVGEID +TIP+++ YT PKQPHYNQDKT+EFIIKLLQF+VPLAI
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYNKS 134
           LGLAVGIR Y KS
Sbjct: 121 LGLAVGIRIYTKS 133

BLAST of Chy5G095970 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 198.7 bits (504), Expect = 2.9e-51
Identity = 88/132 (66.67%), Postives = 109/132 (82.58%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           M  D K+ +  EVS+HN  KDCWLII+GKVY+VT F++DHPGGDEVLLS TGKDAT+DFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  DVGHSESAREMMSKYYVGEIDISTIPERLTYTGPKQPHYNQDKTTEFIIKLLQFMVPLAI 120
           DVGHS++AR+MM KY++GEID S++P   TY  P+QP YNQDKT EFIIK+LQF+VP+ I
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYVAPQQPAYNQDKTPEFIIKILQFLVPILI 120

Query: 121 LGLAVGIRFYNK 133
           LGLA+ +R Y K
Sbjct: 121 LGLALVVRHYTK 132

BLAST of Chy5G095970 vs. TAIR 10
Match: AT5G48810.1 (cytochrome B5 isoform D )

HSP 1 Score: 193.7 bits (491), Expect = 9.3e-50
Identity = 85/135 (62.96%), Postives = 113/135 (83.70%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGGDGK+FTL+EVSQH++ KDCW++I+GKVY+VT+FL+DHPGGDEV+L+ TGKDATDDFE
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 61  DVGHSESAREMMSKYYVGEIDISTIPERLTYTGPKQPH--YNQDKTTEFIIKLLQFMVPL 120
           DVGHS +A+ M+ +YYVG+ID +T+P +  +  P        QDK+++F+IKLLQF+VPL
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAVATQDKSSDFVIKLLQFLVPL 120

Query: 121 AILGLAVGIRFYNKS 134
            ILGLA GIR+Y K+
Sbjct: 121 LILGLAFGIRYYTKT 135

BLAST of Chy5G095970 vs. TAIR 10
Match: AT2G46650.1 (cytochrome B5 isoform C )

HSP 1 Score: 132.9 bits (333), Expect = 2.0e-31
Identity = 60/127 (47.24%), Postives = 89/127 (70.08%), Query Frame = 0

Query: 7   LFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFEDVGHSE 66
           L +  +V++H    DCW++I+GKVY+++ F+++HPGGD VLL+ TGKDA+ DFEDV HS+
Sbjct: 4   LISFHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSK 63

Query: 67  SAREMMSKYYVGEIDISTIPERLTYTGPKQPHYNQDKTT--EFIIKLLQFMVPLAILGLA 126
            A+E+M KY +G++D ST+P    Y  P +      +TT  E   KLL +++PL ILG+A
Sbjct: 64  DAKELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAETTKEESGKKLLIYLIPLLILGVA 123

Query: 127 VGIRFYN 132
             +RFYN
Sbjct: 124 FALRFYN 130

BLAST of Chy5G095970 vs. TAIR 10
Match: AT1G26340.1 (cytochrome B5 isoform A )

HSP 1 Score: 128.6 bits (322), Expect = 3.7e-30
Identity = 56/127 (44.09%), Postives = 91/127 (71.65%), Query Frame = 0

Query: 6   KLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFEDVGHS 65
           KL+++ E + HN   DCW++I+GKVY+V+ ++++HPGGD+VLL+  GKDATDDFED GHS
Sbjct: 6   KLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHS 65

Query: 66  ESAREMMSKYYVGEIDISTIPERLTYTGPKQPHYNQDKTTEFIIKLLQ-----FMVPLAI 125
           + ARE+M KY++GE+D S++PE      P+   Y +D+  + + KL       ++VP++I
Sbjct: 66  KDARELMEKYFIGELDESSLPEI-----PELKIYKKDQPQDSVQKLFDLTKQYWVVPVSI 125

Query: 126 LGLAVGI 128
           + ++V +
Sbjct: 126 ITISVAV 127

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O488451.1e-6079.70Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1[more]
P490996.3e-5976.87Cytochrome b5, seed isoform OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
P490982.4e-5875.37Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
O043541.9e-5575.38Cytochrome b5 OS=Borago officinalis OX=13363 PE=2 SV=1[more]
P491004.7e-5475.78Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A5D3CSF52.0e-7197.01Cytochrome b5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold472G0... [more]
A0A1S3C5R92.0e-7197.01cytochrome b5-like OS=Cucumis melo OX=3656 GN=LOC103496974 PE=3 SV=1[more]
A0A0A0LHS95.7e-7197.01Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 ... [more]
A0A6J1CAX13.2e-6689.55cytochrome b5-like OS=Momordica charantia OX=3673 GN=LOC111009917 PE=3 SV=1[more]
A0A6J1KXV92.7e-6590.30cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111499200 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_008457249.11.16e-9397.01PREDICTED: cytochrome b5-like [Cucumis melo] >KAA0033284.1 cytochrome b5-like [C... [more]
XP_004138957.14.71e-9397.01cytochrome b5 [Cucumis sativus] >KGN61465.1 hypothetical protein Csa_005989 [Cuc... [more]
XP_022138846.14.44e-8689.55cytochrome b5-like [Momordica charantia][more]
XP_023006481.11.40e-8590.30cytochrome b5-like [Cucurbita maxima][more]
XP_038906915.12.83e-8592.54cytochrome b5-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT2G32720.18.1e-6279.70cytochrome B5 isoform B [more]
AT5G53560.12.9e-5166.67cytochrome B5 isoform E [more]
AT5G48810.19.3e-5062.96cytochrome B5 isoform D [more]
AT2G46650.12.0e-3147.24cytochrome B5 isoform C [more]
AT1G26340.13.7e-3044.09cytochrome B5 isoform A [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001199Cytochrome b5-like heme/steroid binding domainPRINTSPR00363CYTOCHROMEB5coord: 68..80
score: 39.04
coord: 40..54
score: 53.54
coord: 30..40
score: 61.02
coord: 55..62
score: 48.11
IPR001199Cytochrome b5-like heme/steroid binding domainSMARTSM01117Cyt_b5_2coord: 8..81
e-value: 9.5E-36
score: 134.8
IPR001199Cytochrome b5-like heme/steroid binding domainPFAMPF00173Cyt-b5coord: 9..80
e-value: 4.8E-28
score: 97.1
IPR001199Cytochrome b5-like heme/steroid binding domainPROSITEPS50255CYTOCHROME_B5_2coord: 5..81
score: 29.95039
IPR036400Cytochrome b5-like heme/steroid binding domain superfamilyGENE3D3.10.120.10coord: 3..85
e-value: 1.7E-35
score: 122.9
IPR036400Cytochrome b5-like heme/steroid binding domain superfamilySUPERFAMILY55856Cytochrome b5-like heme/steroid binding domaincoord: 6..90
NoneNo IPR availablePANTHERPTHR19359CYTOCHROME B5coord: 1..132
NoneNo IPR availablePANTHERPTHR19359:SF110BNAC04G43300D PROTEINcoord: 1..132
IPR018506Cytochrome b5, heme-binding sitePROSITEPS00191CYTOCHROME_B5_1coord: 36..43

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy5G095970.1Chy5G095970.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043447 alkane biosynthetic process
biological_process GO:0042761 very long-chain fatty acid biosynthetic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0043231 intracellular membrane-bounded organelle
molecular_function GO:0020037 heme binding
molecular_function GO:0046872 metal ion binding