Chy5G094230 (gene) Cucumber (hystrix) v1

Overview
NameChy5G094230
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionSulfate adenylyltransferase
LocationchrH05: 4074431 .. 4078256 (-)
RNA-Seq ExpressionChy5G094230
SyntenyChy5G094230
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGTCCATGGCCGCCGCCTTCATGAAAACTTCAAATCTCTTCCCCCAGTCTCCAATTACATTCACTAAAGCCGTAACACCGCCGATTATGCTCCCAATGTCTCTCAGAGTAAAAACCAAAACCCACCGTCGGATCCGCGTCTCGTGCGGGTTGATCGACCCGGACGGGGGAAAGCTTGTGGAACTTATTGTGGAGGAATCCATGAGGGGTTCCAAGAATCGGGAGGCTCTGAGTCTGCCCCGAATCAAGCTGTCGCGAATCGATCTGCAATGGGTTCACGTTCTGAGCGAGGGCTGGGCTAGCCCTCTTACCGGATTCATGAGAGAATCCGAGTTCCTCCAAACGCTTCATTTCAATTCGCTCCGTCTTCAAGATGGATCGGTTGTTAACATGTCGGTGCCGATCGTTTTGGCCATCGACGATGACCTCAAGAACCGAATCGGCGACTCCTCTATGGTTGCGCTCTTCGGCTCCGATGATCGCCCAGTTGCAATTCTCAATGAGTAAGATTATTATTTTCTTCAACTCTATTTTTTTATTTCTCTTTCATATATATAATTTTATTTTAGGCATTTTCAAGTATACCAACTAAAATCAATATTTACAAAATATATCAAAATTATTATATATATATTTTTTTAAGTATAGTTTGAATTTAATTTTAATTTGGAACTATGAATTCATGTCATTTTCAACTATGTTTTGATCGGCCATGTTATGAACTCTCATCCCACGCGTTGTTAAGGAAAAAGAATCTAACTTTAATACAAAAATTACTTTTCTTTCTTGTTTAGTTGACTTTGTAGAGGATCAACCTTACTTTAGCTATTTAAGTTTTTATTAGGGCCCATCTTGGTGTTTAAGGTTAATTTTCAATTAATTATGACTAATTTCGTGTTACAATCACATTAGTTTTAAAATAAGTTATGAAGCAAATTTTTTTTTTAATTAGAATGAAAAGTAAGAAAAACGAATTTATTTTAGTTAGTTTAATTTTTTAAAAATTGATTAACCTATATTAACGCCAACAATATCTAAAGCGAGTATGTAGTGAAGACTTAAGGTTAAAAGTATAAATCTTGAATCAACAAAAACTAAATTAAAACAACACACAAAATTCGAAAGTAGCAATCAAATGAAAATTACTTCAAATTTTAGTAGAAAAAGTTTAATATTTTGAAGATGTAAATGAAAATTAGAGCATAGATGGTTATGTTAGGGACCAAATTTATCGTTCCCTACTTTATATTATATTTAAATTTGAGTTTGGTTTTAGTATATTCGGTTAAATTTACATATAGTGTAGAGATTGTAAATTTCTAATAAACTGATGTGAGTCTCCCAATTGAATAAAAGTCCAGAAAATGGAGCCATACCCATAGGAATAGACACGTGTATTGCTTAGAAAAATATTTATTGAAAGATTTTAAGAAATAATTTATGTAAGTTTAGTACTTTTACTTTAATTTTGTGTATATGTTTTATTTGCCCTTTAAATTTTAAAATATTACATTTTTATTTAGAGATATTTTTCAAAAATAGTAAAATATTGTAATTATTTATTAAATATAACAAAACTCGTCAAGGTCTTCATAGTCTATTTAAGTTTTGTTTTTATATTTTGTAAATAGTTAAATTTTCTTTTGCCATTTATGACAATTTCGATTTGAATATTTCATTAATTTTAATGAAATAGGATTAATTAATTTAAATATCTGTATCATACTTGTTTCACTCGATTGTCTAATTAAATTATTTTATTTAAGGAACAAAAGCTAGCATTAGTGTAAAAATCTAGAAATTTAGGGATCGGATAAAAACTAAACTCGATACTCGAGATTAAAATACAACATTTTAAAATATATAAACCAAATGAAAGCTAACCTTAAAATTTATATACATCTTGAAATCCAGGAAAGAAATGAAAACATAATTAAAAACTTTGGAAACAAAAAGTTATTTTCTCCTTATATTTATGGTCTTTTTATTGCATCCATTTCAATATAAATTTGAAGGTGTTTTTTAATTAATGTTTCTTAAACAAGTAGCATTAAATCTTTTTCCTCCTAATCTTTTTTGGTAGACAGTATAAAATCTATAATTTAGAGATGTAAAATTATAAGCAAATGAGAAATTAAGTGTGAACATAAAAATATGTAAAATTGAAATAATATGTATACTAATCTTTTTTTTCCTTGAGCTCACATTATCTATTAATGATGGACTTTTTTAAAATTAAAAATGAAAAGGATACAATACATTGGTGTTGGGATTATAGTGATGTCATAATTAGTTGGTTTTGTTTTCTTGTTTTTTTTTTTCTCTATTTGAACATTGGTTGGTAGTCGATTGATTTGATTCAATTCTTGGTTGGTCCCAAAACTAACAAAATCGAACTGTCTTTCACTGCTTCTATATATTATATTGGTTTTGTTAGTTTTGAAACTAACCAAAAACTGAACTGGCTTTCACCCTTAGTTCTTATAGTTAAATCATAGCAATATACGCTTTGATGGTTTAATTGATGTTATAATAGAAATCCATAAGGGGAGTGAAGTAGTTTGTCTGATGGTTTTTATTGGATTAATCAGTATTGAGATTTACAAGCACCCCAAGGAAGAAAGGATTGCAAGAACATGGGGAACAACAGCTTCAGGTCTCCCTTATGTTGATGAAGCTATTACGAATGCTGGAAATTGGCTAATAGGAGGTGATCTTGAGGTCATAGAACCAATTAAATACCACGATGGCCTTGATCGTTTTCGATTATCACCTGCACAACTTCGCAACGAGTTCACCAGCCGCAATGCCGATGCAGTGTTTGCTTTCCAGCTTCGCAACCCCGTACATAATGGTCACGCTCTTCTCATGACTGATACTCGTCGTCGTCTTCTTAATATGGGCTACAACAACCCCATCCTCTTGCTTAATCCATTGGGTGGATACACCAAGGCTGATGATGTTCCTCTTCACTGGAGGATCAAACAACACCAAAAGGTTCCCAGCTGGGCACCTCCCTTCATATCATATTTCCATTTTCCTCACCATAATGCAACTAAGTCAACTTTTGTAACTCACTTCATTAGGTTCTTGAGGATGGTGTTCTTGATCCCGAGACAACTGTTGTCTCCATTTTCCCGTCACCAATGCATTATGCTGGTCCAACTGAGGTCCAGTGGCACGCAAAGGCAAGGATCAATGCAGGGGCTAACTTTTACATCGTTGGTCGTGATCCGGCAGGCATGGGCCATCCAACTGAGAAAAGGGATTTGTATGATGCTGACCATGGGAAGAAAGTATTGAGCATGGCACCCGGTTTAGAGCGATTAAACATCCTTCCTTTCAAGGTTTTGCTGATTTGAACTTCTAACTCTTTTTTGCCACAATCACTTAATCTTCATTGCCTTTCCTATTAATCCCAACTTTCAACTTTAATATAGGTTGCTGCATATGACAAAACTCAAGGAAAAATGGCCTTTTTTGATCCCTCAAGACCTCAGGATTTCCTTTTCATATCAGGCACCAAGGTACTTTCTCATACCAATCTCCCCAATTCAAGAGAATCGATGCCAGCTTCGTAGCTTTTCCAACACAAAATAACTGTCTTTTGTCTCTAAAACTAAAACAAAAAAGAGGGAATAGAGATTCTGAGATGGAGTCTTTTCTTGTTATTTGTTAGATGCGAAATCTCGCAAGGAATAAGGAGAATCCACCAGATGGATTTATGTGTCCCAGTGGCTGGAACGTATTGGTCGAATACTACAAAAGTTTAGTTCCAGCAGACAATGGGAGGATCCCAAAGCCAGAAGCTGTAGCAGCTTAA

mRNA sequence

ATGGCGTCCATGGCCGCCGCCTTCATGAAAACTTCAAATCTCTTCCCCCAGTCTCCAATTACATTCACTAAAGCCGTAACACCGCCGATTATGCTCCCAATGTCTCTCAGAGTAAAAACCAAAACCCACCGTCGGATCCGCGTCTCGTGCGGGTTGATCGACCCGGACGGGGGAAAGCTTGTGGAACTTATTGTGGAGGAATCCATGAGGGGTTCCAAGAATCGGGAGGCTCTGAGTCTGCCCCGAATCAAGCTGTCGCGAATCGATCTGCAATGGGTTCACGTTCTGAGCGAGGGCTGGGCTAGCCCTCTTACCGGATTCATGAGAGAATCCGAGTTCCTCCAAACGCTTCATTTCAATTCGCTCCGTCTTCAAGATGGATCGGTTGTTAACATGTCGGTGCCGATCGTTTTGGCCATCGACGATGACCTCAAGAACCGAATCGGCGACTCCTCTATGGTTGCGCTCTTCGGCTCCGATGATCGCCCAGTTGCAATTCTCAATGATATTGAGATTTACAAGCACCCCAAGGAAGAAAGGATTGCAAGAACATGGGGAACAACAGCTTCAGGTCTCCCTTATGTTGATGAAGCTATTACGAATGCTGGAAATTGGCTAATAGGAGGTGATCTTGAGGTCATAGAACCAATTAAATACCACGATGGCCTTGATCGTTTTCGATTATCACCTGCACAACTTCGCAACGAGTTCACCAGCCGCAATGCCGATGCAGTGTTTGCTTTCCAGCTTCGCAACCCCGTACATAATGGTCACGCTCTTCTCATGACTGATACTCGTCGTCGTCTTCTTAATATGGGCTACAACAACCCCATCCTCTTGCTTAATCCATTGGGTGGATACACCAAGGCTGATGATGTTCCTCTTCACTGGAGGATCAAACAACACCAAAAGGTTCTTGAGGATGGTGTTCTTGATCCCGAGACAACTGTTGTCTCCATTTTCCCGTCACCAATGCATTATGCTGGTCCAACTGAGGTCCAGTGGCACGCAAAGGCAAGGATCAATGCAGGGGCTAACTTTTACATCGTTGGTCGTGATCCGGCAGGCATGGGCCATCCAACTGAGAAAAGGGATTTGTATGATGCTGACCATGGGAAGAAAGTATTGAGCATGGCACCCGGTTTAGAGCGATTAAACATCCTTCCTTTCAAGGTTGCTGCATATGACAAAACTCAAGGAAAAATGGCCTTTTTTGATCCCTCAAGACCTCAGGATTTCCTTTTCATATCAGGCACCAAGATGCGAAATCTCGCAAGGAATAAGGAGAATCCACCAGATGGATTTATGTGTCCCAGTGGCTGGAACGTATTGGTCGAATACTACAAAAGTTTAGTTCCAGCAGACAATGGGAGGATCCCAAAGCCAGAAGCTGTAGCAGCTTAA

Coding sequence (CDS)

ATGGCGTCCATGGCCGCCGCCTTCATGAAAACTTCAAATCTCTTCCCCCAGTCTCCAATTACATTCACTAAAGCCGTAACACCGCCGATTATGCTCCCAATGTCTCTCAGAGTAAAAACCAAAACCCACCGTCGGATCCGCGTCTCGTGCGGGTTGATCGACCCGGACGGGGGAAAGCTTGTGGAACTTATTGTGGAGGAATCCATGAGGGGTTCCAAGAATCGGGAGGCTCTGAGTCTGCCCCGAATCAAGCTGTCGCGAATCGATCTGCAATGGGTTCACGTTCTGAGCGAGGGCTGGGCTAGCCCTCTTACCGGATTCATGAGAGAATCCGAGTTCCTCCAAACGCTTCATTTCAATTCGCTCCGTCTTCAAGATGGATCGGTTGTTAACATGTCGGTGCCGATCGTTTTGGCCATCGACGATGACCTCAAGAACCGAATCGGCGACTCCTCTATGGTTGCGCTCTTCGGCTCCGATGATCGCCCAGTTGCAATTCTCAATGATATTGAGATTTACAAGCACCCCAAGGAAGAAAGGATTGCAAGAACATGGGGAACAACAGCTTCAGGTCTCCCTTATGTTGATGAAGCTATTACGAATGCTGGAAATTGGCTAATAGGAGGTGATCTTGAGGTCATAGAACCAATTAAATACCACGATGGCCTTGATCGTTTTCGATTATCACCTGCACAACTTCGCAACGAGTTCACCAGCCGCAATGCCGATGCAGTGTTTGCTTTCCAGCTTCGCAACCCCGTACATAATGGTCACGCTCTTCTCATGACTGATACTCGTCGTCGTCTTCTTAATATGGGCTACAACAACCCCATCCTCTTGCTTAATCCATTGGGTGGATACACCAAGGCTGATGATGTTCCTCTTCACTGGAGGATCAAACAACACCAAAAGGTTCTTGAGGATGGTGTTCTTGATCCCGAGACAACTGTTGTCTCCATTTTCCCGTCACCAATGCATTATGCTGGTCCAACTGAGGTCCAGTGGCACGCAAAGGCAAGGATCAATGCAGGGGCTAACTTTTACATCGTTGGTCGTGATCCGGCAGGCATGGGCCATCCAACTGAGAAAAGGGATTTGTATGATGCTGACCATGGGAAGAAAGTATTGAGCATGGCACCCGGTTTAGAGCGATTAAACATCCTTCCTTTCAAGGTTGCTGCATATGACAAAACTCAAGGAAAAATGGCCTTTTTTGATCCCTCAAGACCTCAGGATTTCCTTTTCATATCAGGCACCAAGATGCGAAATCTCGCAAGGAATAAGGAGAATCCACCAGATGGATTTATGTGTCCCAGTGGCTGGAACGTATTGGTCGAATACTACAAAAGTTTAGTTCCAGCAGACAATGGGAGGATCCCAAAGCCAGAAGCTGTAGCAGCTTAA

Protein sequence

MASMAAAFMKTSNLFPQSPITFTKAVTPPIMLPMSLRVKTKTHRRIRVSCGLIDPDGGKLVELIVEESMRGSKNREALSLPRIKLSRIDLQWVHVLSEGWASPLTGFMRESEFLQTLHFNSLRLQDGSVVNMSVPIVLAIDDDLKNRIGDSSMVALFGSDDRPVAILNDIEIYKHPKEERIARTWGTTASGLPYVDEAITNAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAQLRNEFTSRNADAVFAFQLRNPVHNGHALLMTDTRRRLLNMGYNNPILLLNPLGGYTKADDVPLHWRIKQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPTEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGTKMRNLARNKENPPDGFMCPSGWNVLVEYYKSLVPADNGRIPKPEAVAA*
Homology
BLAST of Chy5G094230 vs. ExPASy Swiss-Prot
Match: Q9LIK9 (ATP sulfurylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS1 PE=1 SV=1)

HSP 1 Score: 761.9 bits (1966), Expect = 4.2e-219
Identity = 370/461 (80.26%), Postives = 398/461 (86.33%), Query Frame = 0

Query: 1   MASMAAAFMKTSNLFPQSPITFTKAVTPPIMLPMSLRVKTKTHRRIRVSCGLIDPDGGKL 60
           MASMAA   KT   F   P+T +   +      +S   K+   R   +  GLI PDGGKL
Sbjct: 1   MASMAAVLSKTP--FLSQPLTKSSPNSDLPFAAVSFPSKSLRRRVGSIRAGLIAPDGGKL 60

Query: 61  VELIVEESMRGSKNREALSLPRIKLSRIDLQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120
           VELIVEE  R  K  EA  LPR++L+ IDLQW+HVLSEGWASPL GFMRESEFLQTLHFN
Sbjct: 61  VELIVEEPKRREKKHEAADLPRVELTAIDLQWMHVLSEGWASPLGGFMRESEFLQTLHFN 120

Query: 121 SLRLQDGSVVNMSVPIVLAIDDDLKNRIGDSSMVALFGSDDRPVAILNDIEIYKHPKEER 180
           SLRL DGSVVNMSVPIVLAIDD+ K RIG+S+ VALF SD  PVAIL+DIEIYKHPKEER
Sbjct: 121 SLRLDDGSVVNMSVPIVLAIDDEQKARIGESTRVALFNSDGNPVAILSDIEIYKHPKEER 180

Query: 181 IARTWGTTASGLPYVDEAITNAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAQLRNEFTSR 240
           IARTWGTTA GLPYVDEAITNAGNWLIGGDLEV+EP+KY+DGLDRFRLSPA+LR E   R
Sbjct: 181 IARTWGTTAPGLPYVDEAITNAGNWLIGGDLEVLEPVKYNDGLDRFRLSPAELRKELEKR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLNMGYNNPILLLNPLGGYTKADDVPLHWRIK 300
           NADAVFAFQLRNPVHNGHALLMTDTRRRLL MGY NPILLL+PLGG+TKADDVPL WR+K
Sbjct: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGFTKADDVPLDWRMK 300

Query: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QH+KVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420
            EKRDLYDADHGKKVLSMAPGLERLNILPF+VAAYDKTQGKMAFFDPSRPQDFLFISGTK
Sbjct: 361 VEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420

Query: 421 MRNLARNKENPPDGFMCPSGWNVLVEYYKSLVPADNGRIPK 462
           MR LA+N ENPPDGFMCP GW VLV+YY+SL PA NGR+P+
Sbjct: 421 MRTLAKNNENPPDGFMCPGGWKVLVDYYESLTPAGNGRLPE 459

BLAST of Chy5G094230 vs. ExPASy Swiss-Prot
Match: O23324 (ATP-sulfurylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS3 PE=1 SV=1)

HSP 1 Score: 732.3 bits (1889), Expect = 3.6e-210
Identity = 357/464 (76.94%), Postives = 393/464 (84.70%), Query Frame = 0

Query: 1   MASMAAAFMKTSNLFPQSPIT---FTKAVTPPIMLPMSLRVKTKTHRRIRVSCGLIDPDG 60
           MASM+  F K ++   Q P+T    + +VT  I  P +   KT++ R I V  GLI+PDG
Sbjct: 1   MASMSTVFPKPTSFISQ-PLTKSHKSDSVTTSISFPSN--SKTRSLRTISVRAGLIEPDG 60

Query: 61  GKLVELIVEESMRGSKNREALSLPRIKLSRIDLQWVHVLSEGWASPLTGFMRESEFLQTL 120
           GKLV+L+V E  R  K  EA  LPR++L+ IDLQW+HVLSEGWASPL GFMRESEFLQTL
Sbjct: 61  GKLVDLVVPEPRRREKKHEAADLPRVRLTAIDLQWMHVLSEGWASPLRGFMRESEFLQTL 120

Query: 121 HFNSLRLQDGSVVNMSVPIVLAIDDDLKNRIGDSSMVALFGSDDRPVAILNDIEIYKHPK 180
           HFN L L DGSVVNMSVPIVLAIDD  K  IG+S  V+L  SDD P+AILNDIEIYKHPK
Sbjct: 121 HFNLLNLDDGSVVNMSVPIVLAIDDQQKALIGESKRVSLVDSDDNPIAILNDIEIYKHPK 180

Query: 181 EERIARTWGTTASGLPYVDEAITNAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAQLRNEF 240
           EERIARTWGTTA GLPYV+EAITNAG+WLIGGDLEV+EP+KY+DGLDRFRLSP +LR E 
Sbjct: 181 EERIARTWGTTAPGLPYVEEAITNAGDWLIGGDLEVLEPVKYNDGLDRFRLSPFELRKEL 240

Query: 241 TSRNADAVFAFQLRNPVHNGHALLMTDTRRRLLNMGYNNPILLLNPLGGYTKADDVPLHW 300
             R ADAVFAFQLRNPVHNGHALLMTDTRRRLL MGY NPILLL+PLGG+TKADDVPL W
Sbjct: 241 EKRGADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGFTKADDVPLSW 300

Query: 301 RIKQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGM 360
           R+KQH+KVLEDGVLDPETTVVSIFPSPM YAGPTEVQWHAKARINAGANFYIVGRDPAGM
Sbjct: 301 RMKQHEKVLEDGVLDPETTVVSIFPSPMLYAGPTEVQWHAKARINAGANFYIVGRDPAGM 360

Query: 361 GHPTEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFIS 420
           GHP EKRDLYDADHGKKVLSMAPGLERLNILPF+VAAYDKTQGKMAFFDPSR QDFLFIS
Sbjct: 361 GHPVEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFIS 420

Query: 421 GTKMRNLARNKENPPDGFMCPSGWNVLVEYYKSLVPADNGRIPK 462
           GTKMR LA+N+ENPPDGFMCP GW VLV+YY SL    N ++P+
Sbjct: 421 GTKMRALAKNRENPPDGFMCPGGWKVLVDYYDSLTLTGNTKLPE 461

BLAST of Chy5G094230 vs. ExPASy Swiss-Prot
Match: Q9S7D8 (ATP sulfurylase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS4 PE=2 SV=1)

HSP 1 Score: 726.9 bits (1875), Expect = 1.5e-208
Identity = 362/471 (76.86%), Postives = 396/471 (84.08%), Query Frame = 0

Query: 1   MASMAAAFMKTSNLFPQSPITFTKAVT--PPIMLPMSLRVKTKTHRRIRVSCGLIDPDGG 60
           MAS AAA +  S  F  SP+      +   P  + +    +  + R + V  GLI+PDGG
Sbjct: 1   MASSAAAIVSGSP-FRSSPLIHNHHASRYAPGSISVVSLPRQVSRRGLSVKSGLIEPDGG 60

Query: 61  KLVELIVEESMRGSKNREALSLP-RIKLSRIDLQWVHVLSEGWASPLTGFMRESEFLQTL 120
           KL+ L+VEES R     EA ++P RIKL+R+DL+WVHVLSEGWASPL GFMR+SEFLQTL
Sbjct: 61  KLMNLVVEESRRRVMKHEAETVPARIKLNRVDLEWVHVLSEGWASPLKGFMRQSEFLQTL 120

Query: 121 HFNSLRLQDGSVVNMSVPIVLAIDDDLKNRIGDSSMVALFGSDDRPVAILNDIEIYKHPK 180
           HFNS RL+DGSVVNMSVPIVLAIDDD K RIGDS+ V L  S   P+AILNDIEIYKHPK
Sbjct: 121 HFNSFRLEDGSVVNMSVPIVLAIDDDQKFRIGDSNQVTLVDSVGNPIAILNDIEIYKHPK 180

Query: 181 EERIARTWGTTASGLPYVDEAITNAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAQLRNEF 240
           EERIARTWGTTA GLPY +EAIT AGNWLIGGDL+V+EPIKY+DGLDRFRLSP+QLR EF
Sbjct: 181 EERIARTWGTTARGLPYAEEAITKAGNWLIGGDLQVLEPIKYNDGLDRFRLSPSQLREEF 240

Query: 241 TSRNADAVFAFQLRNPVHNGHALLMTDTRRRLLNMGYNNPILLLNPLGGYTKADDVPLHW 300
             R ADAVFAFQLRNPVHNGHALLMTDTRRRLL MGY NP+LLLNPLGG+TKADDVPL W
Sbjct: 241 IRRGADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPVLLLNPLGGFTKADDVPLSW 300

Query: 301 RIKQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGM 360
           R++QH+KVLEDGVLDPETTVVSIFPSPM YAGPTEVQWHAKARINAGANFYIVGRDPAGM
Sbjct: 301 RMRQHEKVLEDGVLDPETTVVSIFPSPMLYAGPTEVQWHAKARINAGANFYIVGRDPAGM 360

Query: 361 GHPTEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFIS 420
           GHPTEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSR QDFLFIS
Sbjct: 361 GHPTEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRSQDFLFIS 420

Query: 421 GTKMRNLARNKENPPDGFMCPSGWNVLVEYYKSL-VPADNGRIPKPEAVAA 468
           GTKMR LA+ KENPPDGFMCPSGW VLV+YY SL     NGR+   EAVA+
Sbjct: 421 GTKMRGLAKKKENPPDGFMCPSGWKVLVDYYDSLSAETGNGRV--SEAVAS 468

BLAST of Chy5G094230 vs. ExPASy Swiss-Prot
Match: Q43870 (ATP sulfurylase 2 OS=Arabidopsis thaliana OX=3702 GN=APS2 PE=1 SV=1)

HSP 1 Score: 658.3 bits (1697), Expect = 6.5e-188
Identity = 308/407 (75.68%), Postives = 352/407 (86.49%), Query Frame = 0

Query: 45  RIRVSCGLIDPDGGKLVELIVEESMRGSKNREALSLPRIKLSRIDLQWVHVLSEGWASPL 104
           ++ V   LIDPDGG+LVELIV E+  G K  E+ ++P++KL++IDL+WVHV+SEGWASPL
Sbjct: 57  KMTVKSSLIDPDGGELVELIVPETEIGVKKAESETMPKVKLNQIDLEWVHVISEGWASPL 116

Query: 105 TGFMRESEFLQTLHFNSLRLQDGSVVNMSVPIVLAIDDDLKNRIGDSSMVALFGSDDRPV 164
            GFMRE E+LQ+LHFNSLRL++G+ VNMS+PIVLAIDDD K +IG S  VAL       +
Sbjct: 117 KGFMREDEYLQSLHFNSLRLKNGTFVNMSLPIVLAIDDDTKEQIGSSENVALVCPQGDII 176

Query: 165 AILNDIEIYKHPKEERIARTWGTTASGLPYVDEAITNAGNWLIGGDLEVIEPIKYHDGLD 224
             L  +EIYKH KEERIARTWGTT+ GLPYV+E IT +GNWLIGGDLEV EPIKY+DGLD
Sbjct: 177 GSLRSVEIYKHNKEERIARTWGTTSPGLPYVEEYITPSGNWLIGGDLEVFEPIKYNDGLD 236

Query: 225 RFRLSPAQLRNEFTSRNADAVFAFQLRNPVHNGHALLMTDTRRRLLNMGYNNPILLLNPL 284
            +RLSP QLR EF +R ADAVFAFQLRNPVHNGHALLM DTR+RLL MGY NP+LLL+PL
Sbjct: 237 HYRLSPKQLREEFDNRQADAVFAFQLRNPVHNGHALLMNDTRKRLLEMGYKNPVLLLHPL 296

Query: 285 GGYTKADDVPLHWRIKQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAG 344
           GG+TKADDVPL  R++QH KVLEDGVLDP+TT+VSIFPSPMHYAGPTEVQWHAKARINAG
Sbjct: 297 GGFTKADDVPLDVRMEQHSKVLEDGVLDPKTTIVSIFPSPMHYAGPTEVQWHAKARINAG 356

Query: 345 ANFYIVGRDPAGMGHPTEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAF 404
           ANFYIVGRDPAGMGHPTEKRDLYD DHGK+VLSMAPGLE+LNILPF+VAAYD  + KMAF
Sbjct: 357 ANFYIVGRDPAGMGHPTEKRDLYDPDHGKRVLSMAPGLEKLNILPFRVAAYDTIEKKMAF 416

Query: 405 FDPSRPQDFLFISGTKMRNLARNKENPPDGFMCPSGWNVLVEYYKSL 452
           FDPSR ++FLFISGTKMR  AR  ENPPDGFMCPSGWNVLV+YY+SL
Sbjct: 417 FDPSRAKEFLFISGTKMRTYARTGENPPDGFMCPSGWNVLVKYYESL 463

BLAST of Chy5G094230 vs. ExPASy Swiss-Prot
Match: O95340 (Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2 OS=Homo sapiens OX=9606 GN=PAPSS2 PE=1 SV=2)

HSP 1 Score: 501.1 bits (1289), Expect = 1.3e-140
Identity = 237/391 (60.61%), Postives = 301/391 (76.98%), Query Frame = 0

Query: 62  ELIVEESMRGSKNREALSLPRIKLSRIDLQWVHVLSEGWASPLTGFMRESEFLQTLHFNS 121
           EL V E+       EA +LP + ++++DLQWV VLSEGWA+PL GFMRE E+LQ +HF++
Sbjct: 226 ELFVPENKLDHVRAEAETLPSLSITKLDLQWVQVLSEGWATPLKGFMREKEYLQVMHFDT 285

Query: 122 LRLQDGSVVNMSVPIVLAIDDDLKNRI-GDSSMVALFGSDDRPVAILNDIEIYKHPKEER 181
           L L DG V+NMS+PIVL +  + K R+ G S  V   G   R VAIL D E Y+H KEER
Sbjct: 286 L-LDDG-VINMSIPIVLPVSAEDKTRLEGCSKFVLAHGG--RRVAILRDAEFYEHRKEER 345

Query: 182 IARTWGTTASGLPYVDEAITNAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAQLRNEFTSR 241
            +R WGTT +  P++ + +  +G+WL+GGDL+V+E I+++DGLD++RL+P +L+ +    
Sbjct: 346 CSRVWGTTCTKHPHI-KMVMESGDWLVGGDLQVLEKIRWNDGLDQYRLTPLELKQKCKEM 405

Query: 242 NADAVFAFQLRNPVHNGHALLMTDTRRRLLNMGYNNPILLLNPLGGYTKADDVPLHWRIK 301
           NADAVFAFQLRNPVHNGHALLM DTRRRLL  GY +P+LLL+PLGG+TK DDVPL WR+K
Sbjct: 406 NADAVFAFQLRNPVHNGHALLMQDTRRRLLERGYKHPVLLLHPLGGWTKDDDVPLDWRMK 465

Query: 302 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 361
           QH  VLE+GVLDP++T+V+IFPSPM YAGPTEVQWH ++R+ AGANFYIVGRDPAGM HP
Sbjct: 466 QHAAVLEEGVLDPKSTIVAIFPSPMLYAGPTEVQWHCRSRMIAGANFYIVGRDPAGMPHP 525

Query: 362 TEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGTK 421
             K+DLY+  HG KVLSMAPGL  + I+PF+VAAY+K +  M F+DP+R  +F FISGT+
Sbjct: 526 ETKKDLYEPTHGGKVLSMAPGLTSVEIIPFRVAAYNKAKKAMDFYDPARHNEFDFISGTR 585

Query: 422 MRNLARNKENPPDGFMCPSGWNVLVEYYKSL 452
           MR LAR  ENPPDGFM P  W VL +YY+SL
Sbjct: 586 MRKLAREGENPPDGFMAPKAWKVLTDYYRSL 611

BLAST of Chy5G094230 vs. ExPASy TrEMBL
Match: A0A0A0LIM7 (Sulfate adenylyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G215520 PE=4 SV=1)

HSP 1 Score: 950.7 bits (2456), Expect = 2.3e-273
Identity = 461/467 (98.72%), Postives = 463/467 (99.14%), Query Frame = 0

Query: 1   MASMAAAFMKTSNLFPQSPITFTKAVTPPIMLPMSLRVKTKTHRRIRVSCGLIDPDGGKL 60
           MASMAAAF+K SNLFPQSPITFTKAVTPPIMLPMSLRVKTKTHRRIRVSCGLIDPDGGKL
Sbjct: 1   MASMAAAFIKPSNLFPQSPITFTKAVTPPIMLPMSLRVKTKTHRRIRVSCGLIDPDGGKL 60

Query: 61  VELIVEESMRGSKNREALSLPRIKLSRIDLQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120
           VELIVEESMRGSKNREALSLPRIKLSRIDLQWVHVLSEGWASPLTGFMRESEFLQTLHFN
Sbjct: 61  VELIVEESMRGSKNREALSLPRIKLSRIDLQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120

Query: 121 SLRLQDGSVVNMSVPIVLAIDDDLKNRIGDSSMVALFGSDDRPVAILNDIEIYKHPKEER 180
           SLRLQDGSVVNMSVPIVLAIDDD KNRIGDSS VALFGSDDRPVAILNDIEIYKHPKEER
Sbjct: 121 SLRLQDGSVVNMSVPIVLAIDDDFKNRIGDSSKVALFGSDDRPVAILNDIEIYKHPKEER 180

Query: 181 IARTWGTTASGLPYVDEAITNAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAQLRNEFTSR 240
           IARTWGTTASGLPYVDEAI NAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAQLRNEFTSR
Sbjct: 181 IARTWGTTASGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAQLRNEFTSR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLNMGYNNPILLLNPLGGYTKADDVPLHWRIK 300
           NADAVFAFQLRNPVHNGHALLMTDTRRRLLNMGYNNPILLLNPLGGYTKADDVPLHWRIK
Sbjct: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLNMGYNNPILLLNPLGGYTKADDVPLHWRIK 300

Query: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420
           TEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGTK
Sbjct: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420

Query: 421 MRNLARNKENPPDGFMCPSGWNVLVEYYKSLVPADNGRIPKPEAVAA 468
           MRNLARNKENPPDGFMCPSGWNVLVEYYKSLVPA+NGRIPKPEAVAA
Sbjct: 421 MRNLARNKENPPDGFMCPSGWNVLVEYYKSLVPAENGRIPKPEAVAA 467

BLAST of Chy5G094230 vs. ExPASy TrEMBL
Match: A0A1S3BMN3 (Sulfate adenylyltransferase OS=Cucumis melo OX=3656 GN=LOC103491225 PE=4 SV=1)

HSP 1 Score: 926.4 bits (2393), Expect = 4.7e-266
Identity = 451/467 (96.57%), Postives = 456/467 (97.64%), Query Frame = 0

Query: 1   MASMAAAFMKTSNLFPQSPITFTKAVTPPIMLPMSLRVKTKTHRRIRVSCGLIDPDGGKL 60
           MASMAAAF+K SN F +SPITF+KAVTPPI LPMSLRVK+ T RRIRVS GLIDPDGGKL
Sbjct: 1   MASMAAAFIKPSNPFSKSPITFSKAVTPPIKLPMSLRVKSNTRRRIRVSGGLIDPDGGKL 60

Query: 61  VELIVEESMRGSKNREALSLPRIKLSRIDLQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120
           VELIVEESMRGSKNREALSLPRIKLSRID+QWVHVLSEGWASPLTGFMRESEFLQTLHFN
Sbjct: 61  VELIVEESMRGSKNREALSLPRIKLSRIDVQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120

Query: 121 SLRLQDGSVVNMSVPIVLAIDDDLKNRIGDSSMVALFGSDDRPVAILNDIEIYKHPKEER 180
           SLRLQDGSVVNMSVPIVLAIDDDLKNRIGDSS VALFGSDDRPVAILNDIEIYKHPKEER
Sbjct: 121 SLRLQDGSVVNMSVPIVLAIDDDLKNRIGDSSKVALFGSDDRPVAILNDIEIYKHPKEER 180

Query: 181 IARTWGTTASGLPYVDEAITNAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAQLRNEFTSR 240
           IARTWGTTASGLPYVDEAI NAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAQLRNEFTSR
Sbjct: 181 IARTWGTTASGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAQLRNEFTSR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLNMGYNNPILLLNPLGGYTKADDVPLHWRIK 300
           NADAVFAFQLRNPVHNGHALLMTDTRRRLLNMGYNNPILLLNPLGGYTKADDVPLHWRIK
Sbjct: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLNMGYNNPILLLNPLGGYTKADDVPLHWRIK 300

Query: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420
            EKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGTK
Sbjct: 361 IEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420

Query: 421 MRNLARNKENPPDGFMCPSGWNVLVEYYKSLVPADNGRIPKPEAVAA 468
           MRNLARNKENPPDGFMCP GWNVLVEYYKSLVPADNGRIPKPEAVAA
Sbjct: 421 MRNLARNKENPPDGFMCPGGWNVLVEYYKSLVPADNGRIPKPEAVAA 467

BLAST of Chy5G094230 vs. ExPASy TrEMBL
Match: A0A6J1H3X2 (Sulfate adenylyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111459921 PE=4 SV=1)

HSP 1 Score: 874.0 bits (2257), Expect = 2.8e-250
Identity = 424/467 (90.79%), Postives = 436/467 (93.36%), Query Frame = 0

Query: 1   MASMAAAFMKTSNLFPQSPITFTKAVTPPIMLPMSLRVKTKTHRRIRVSCGLIDPDGGKL 60
           MASMAAAF+K SN FP SPITF K  +P I LP+SLR+K   HRRIRVSCGLIDPDGGKL
Sbjct: 1   MASMAAAFIKPSNAFPHSPITFIKTTSPSIKLPISLRIKNNAHRRIRVSCGLIDPDGGKL 60

Query: 61  VELIVEESMRGSKNREALSLPRIKLSRIDLQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120
           V+LIV+ESMR  KNREALSLPRIKLSRIDLQWVHVLSEGWASPL+GFMRESEFLQTLHFN
Sbjct: 61  VDLIVDESMRAFKNREALSLPRIKLSRIDLQWVHVLSEGWASPLSGFMRESEFLQTLHFN 120

Query: 121 SLRLQDGSVVNMSVPIVLAIDDDLKNRIGDSSMVALFGSDDRPVAILNDIEIYKHPKEER 180
           SLRL DGSVVNMSVPIVLAIDD +K RIG SS VALFGSDDR VAILNDIEIYK PKEER
Sbjct: 121 SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRLVAILNDIEIYKQPKEER 180

Query: 181 IARTWGTTASGLPYVDEAITNAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAQLRNEFTSR 240
           IARTWGTTA GLPYVDEAI NAGNWLIGGDLEVIEPIKYHDGLD FRLSPAQLRNEF SR
Sbjct: 181 IARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFISR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLNMGYNNPILLLNPLGGYTKADDVPLHWRIK 300
           NA AVFAFQLRNPVHNGHALLM DTRRRLL+MGY NPILLLNPLGGYTKADDVPLHWRIK
Sbjct: 241 NAGAVFAFQLRNPVHNGHALLMNDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRIK 300

Query: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420
           TEKRDLYD DHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGTK
Sbjct: 361 TEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420

Query: 421 MRNLARNKENPPDGFMCPSGWNVLVEYYKSLVPADNGRIPKPEAVAA 468
           MR LARNKENPPDGFMCP GW+VLVEYY+SLVPA+NG IPKPEAVAA
Sbjct: 421 MRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANNGSIPKPEAVAA 467

BLAST of Chy5G094230 vs. ExPASy TrEMBL
Match: A0A6J1L5Q7 (Sulfate adenylyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111499372 PE=4 SV=1)

HSP 1 Score: 872.5 bits (2253), Expect = 8.1e-250
Identity = 419/466 (89.91%), Postives = 438/466 (93.99%), Query Frame = 0

Query: 1   MASMAAAFMKTSNLFPQSPITFTKAVTPPIMLPMSLRVKTKTHRRIRVSCGLIDPDGGKL 60
           MASMAAAF+K +N FPQSPITF K ++P I LP+SLR++    RRIRVSCGLIDPDGGKL
Sbjct: 1   MASMAAAFIKPTNAFPQSPITFVKTISPSIQLPISLRIRNNARRRIRVSCGLIDPDGGKL 60

Query: 61  VELIVEESMRGSKNREALSLPRIKLSRIDLQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120
           ++LIV+ESMR  KNREALSLPR+KLSRIDLQWVH+LSEGWASPL+GFMRESEFLQTLHFN
Sbjct: 61  MDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHILSEGWASPLSGFMRESEFLQTLHFN 120

Query: 121 SLRLQDGSVVNMSVPIVLAIDDDLKNRIGDSSMVALFGSDDRPVAILNDIEIYKHPKEER 180
           SLRL DGSVVNMSVPIVLAIDD +K RIG SS VALFGSDDRPVAILNDIEIYK PKEER
Sbjct: 121 SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRPVAILNDIEIYKQPKEER 180

Query: 181 IARTWGTTASGLPYVDEAITNAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAQLRNEFTSR 240
           IARTWGTTA GLPYVDEAI NAGNWLIGGDLEVIEPIKYHDGLD FRLSPAQLRNEF SR
Sbjct: 181 IARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFISR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLNMGYNNPILLLNPLGGYTKADDVPLHWRIK 300
           NADAVFAFQLRNPVHNGHALLMTDTRRRLL+MGY NPILLLNPLGGYTKADDVPLHWRIK
Sbjct: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRIK 300

Query: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420
           TEKRDLYD DHGKKVLSMAPGLERLNILPFKVAAYDKT+GKMAFFDPSRPQDFLFISGTK
Sbjct: 361 TEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISGTK 420

Query: 421 MRNLARNKENPPDGFMCPSGWNVLVEYYKSLVPADNGRIPKPEAVA 467
           MR LARNKENPPDGFMCP GW+VLVEYY+SLVPA+ G IPKPEAVA
Sbjct: 421 MRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANIGSIPKPEAVA 466

BLAST of Chy5G094230 vs. ExPASy TrEMBL
Match: A0A6J1CE70 (Sulfate adenylyltransferase OS=Momordica charantia OX=3673 GN=LOC111009889 PE=4 SV=1)

HSP 1 Score: 830.9 bits (2145), Expect = 2.7e-237
Identity = 407/469 (86.78%), Postives = 429/469 (91.47%), Query Frame = 0

Query: 1   MASMAAAFMKTSNLFPQSPITFTKAVTPPIMLPMSLRVKTKT--HRRIRVSCGLIDPDGG 60
           MASMAA F K S+   Q+P+ FTK   PP   P +LRV+T T  HR++RVS GLI+PDGG
Sbjct: 1   MASMAAVFCKPSH---QAPLRFTK--IPPSPPPPALRVRTNTTHHRQLRVSAGLIEPDGG 60

Query: 61  KLVELIVEESMRGSKNREALSLPRIKLSRIDLQWVHVLSEGWASPLTGFMRESEFLQTLH 120
           KLVEL+V+ESMRG K REALS PRIKLSRIDLQWVHVLSEGWA+PL+GFMRE EFLQTLH
Sbjct: 61  KLVELLVDESMRGLKKREALSQPRIKLSRIDLQWVHVLSEGWATPLSGFMREPEFLQTLH 120

Query: 121 FNSLRLQDGSVVNMSVPIVLAIDDDLKNRIGDSSMVALFGSDDRPVAILNDIEIYKHPKE 180
           FNSLRL    VVNMSVPIVLAIDD +K+RIG SS VALF SDDRPVAIL DIEIYKHPKE
Sbjct: 121 FNSLRLAGAGVVNMSVPIVLAIDDAVKHRIGASSDVALFASDDRPVAILKDIEIYKHPKE 180

Query: 181 ERIARTWGTTASGLPYVDEAITNAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAQLRNEFT 240
           ERIARTWGTTASGLPY DEAI NAGNWLIGGDLEVIEPIKYHDGLDRFRLSPA+LR EFT
Sbjct: 181 ERIARTWGTTASGLPYADEAIKNAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAELRKEFT 240

Query: 241 SRNADAVFAFQLRNPVHNGHALLMTDTRRRLLNMGYNNPILLLNPLGGYTKADDVPLHWR 300
            RNADAVFAFQLRNPVHNGHALLMTDTRRRLL+MGY NPILLLNPLGGYTKADDVPL+WR
Sbjct: 241 RRNADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYENPILLLNPLGGYTKADDVPLNWR 300

Query: 301 IKQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMG 360
           +KQH+KVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMG
Sbjct: 301 MKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMG 360

Query: 361 HPTEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISG 420
           HPTEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISG
Sbjct: 361 HPTEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISG 420

Query: 421 TKMRNLARNKENPPDGFMCPSGWNVLVEYYKSLVPADNGRIPKPEAVAA 468
           TKMR LARNKENPPDGFMCP GW+VLVEYY SL+PA+NGRIPKPEAV A
Sbjct: 421 TKMRTLARNKENPPDGFMCPGGWSVLVEYYDSLIPANNGRIPKPEAVPA 464

BLAST of Chy5G094230 vs. NCBI nr
Match: XP_004150566.1 (ATP sulfurylase 1, chloroplastic [Cucumis sativus] >KGN61658.1 hypothetical protein Csa_006298 [Cucumis sativus])

HSP 1 Score: 943 bits (2438), Expect = 0.0
Identity = 461/467 (98.72%), Postives = 463/467 (99.14%), Query Frame = 0

Query: 1   MASMAAAFMKTSNLFPQSPITFTKAVTPPIMLPMSLRVKTKTHRRIRVSCGLIDPDGGKL 60
           MASMAAAF+K SNLFPQSPITFTKAVTPPIMLPMSLRVKTKTHRRIRVSCGLIDPDGGKL
Sbjct: 1   MASMAAAFIKPSNLFPQSPITFTKAVTPPIMLPMSLRVKTKTHRRIRVSCGLIDPDGGKL 60

Query: 61  VELIVEESMRGSKNREALSLPRIKLSRIDLQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120
           VELIVEESMRGSKNREALSLPRIKLSRIDLQWVHVLSEGWASPLTGFMRESEFLQTLHFN
Sbjct: 61  VELIVEESMRGSKNREALSLPRIKLSRIDLQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120

Query: 121 SLRLQDGSVVNMSVPIVLAIDDDLKNRIGDSSMVALFGSDDRPVAILNDIEIYKHPKEER 180
           SLRLQDGSVVNMSVPIVLAIDDD KNRIGDSS VALFGSDDRPVAILNDIEIYKHPKEER
Sbjct: 121 SLRLQDGSVVNMSVPIVLAIDDDFKNRIGDSSKVALFGSDDRPVAILNDIEIYKHPKEER 180

Query: 181 IARTWGTTASGLPYVDEAITNAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAQLRNEFTSR 240
           IARTWGTTASGLPYVDEAI NAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAQLRNEFTSR
Sbjct: 181 IARTWGTTASGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAQLRNEFTSR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLNMGYNNPILLLNPLGGYTKADDVPLHWRIK 300
           NADAVFAFQLRNPVHNGHALLMTDTRRRLLNMGYNNPILLLNPLGGYTKADDVPLHWRIK
Sbjct: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLNMGYNNPILLLNPLGGYTKADDVPLHWRIK 300

Query: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420
           TEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGTK
Sbjct: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420

Query: 421 MRNLARNKENPPDGFMCPSGWNVLVEYYKSLVPADNGRIPKPEAVAA 467
           MRNLARNKENPPDGFMCPSGWNVLVEYYKSLVPA+NGRIPKPEAVAA
Sbjct: 421 MRNLARNKENPPDGFMCPSGWNVLVEYYKSLVPAENGRIPKPEAVAA 467

BLAST of Chy5G094230 vs. NCBI nr
Match: XP_008449310.1 (PREDICTED: ATP sulfurylase 1, chloroplastic [Cucumis melo])

HSP 1 Score: 919 bits (2375), Expect = 0.0
Identity = 451/467 (96.57%), Postives = 456/467 (97.64%), Query Frame = 0

Query: 1   MASMAAAFMKTSNLFPQSPITFTKAVTPPIMLPMSLRVKTKTHRRIRVSCGLIDPDGGKL 60
           MASMAAAF+K SN F +SPITF+KAVTPPI LPMSLRVK+ T RRIRVS GLIDPDGGKL
Sbjct: 1   MASMAAAFIKPSNPFSKSPITFSKAVTPPIKLPMSLRVKSNTRRRIRVSGGLIDPDGGKL 60

Query: 61  VELIVEESMRGSKNREALSLPRIKLSRIDLQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120
           VELIVEESMRGSKNREALSLPRIKLSRID+QWVHVLSEGWASPLTGFMRESEFLQTLHFN
Sbjct: 61  VELIVEESMRGSKNREALSLPRIKLSRIDVQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120

Query: 121 SLRLQDGSVVNMSVPIVLAIDDDLKNRIGDSSMVALFGSDDRPVAILNDIEIYKHPKEER 180
           SLRLQDGSVVNMSVPIVLAIDDDLKNRIGDSS VALFGSDDRPVAILNDIEIYKHPKEER
Sbjct: 121 SLRLQDGSVVNMSVPIVLAIDDDLKNRIGDSSKVALFGSDDRPVAILNDIEIYKHPKEER 180

Query: 181 IARTWGTTASGLPYVDEAITNAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAQLRNEFTSR 240
           IARTWGTTASGLPYVDEAI NAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAQLRNEFTSR
Sbjct: 181 IARTWGTTASGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAQLRNEFTSR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLNMGYNNPILLLNPLGGYTKADDVPLHWRIK 300
           NADAVFAFQLRNPVHNGHALLMTDTRRRLLNMGYNNPILLLNPLGGYTKADDVPLHWRIK
Sbjct: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLNMGYNNPILLLNPLGGYTKADDVPLHWRIK 300

Query: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420
            EKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGTK
Sbjct: 361 IEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420

Query: 421 MRNLARNKENPPDGFMCPSGWNVLVEYYKSLVPADNGRIPKPEAVAA 467
           MRNLARNKENPPDGFMCP GWNVLVEYYKSLVPADNGRIPKPEAVAA
Sbjct: 421 MRNLARNKENPPDGFMCPGGWNVLVEYYKSLVPADNGRIPKPEAVAA 467

BLAST of Chy5G094230 vs. NCBI nr
Match: XP_038907157.1 (ATP sulfurylase 1, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 879 bits (2270), Expect = 0.0
Identity = 434/467 (92.93%), Postives = 444/467 (95.07%), Query Frame = 0

Query: 1   MASMAAAFMKTSNLFPQSPITFTKAVTPPIMLPMSLRVKTKTHRRIRVSCGLIDPDGGKL 60
           MASMAAAF+K SN   QSPITFTK  TPPI LP+ L +KT  H RIRVS GLIDPDGGKL
Sbjct: 1   MASMAAAFIKPSNPISQSPITFTKTFTPPIKLPIPL-IKTSRHHRIRVSGGLIDPDGGKL 60

Query: 61  VELIVEESMRGSKNREALSLPRIKLSRIDLQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120
           VELIVEESMR SKNREALSLPRIKLSRIDLQWVHVLSEGWASPLTGFMRESEFLQTLHFN
Sbjct: 61  VELIVEESMRASKNREALSLPRIKLSRIDLQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120

Query: 121 SLRLQDGSVVNMSVPIVLAIDDDLKNRIGDSSMVALFGSDDRPVAILNDIEIYKHPKEER 180
           SLR+ +G VVNMSVPIVLAIDDDLKNRI  SS VALFGSDDRPVAIL +IEIYKHPKEER
Sbjct: 121 SLRV-NGLVVNMSVPIVLAIDDDLKNRIAASSKVALFGSDDRPVAILKNIEIYKHPKEER 180

Query: 181 IARTWGTTASGLPYVDEAITNAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAQLRNEFTSR 240
           IARTWGTTASGLPYVDEAI NAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAQLRNEFTSR
Sbjct: 181 IARTWGTTASGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAQLRNEFTSR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLNMGYNNPILLLNPLGGYTKADDVPLHWRIK 300
           NADAVFAFQLRNPVHNGHALLMTDTRRRLLNMGYNNPILLLNPLGGYTKADDVPLHWRIK
Sbjct: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLNMGYNNPILLLNPLGGYTKADDVPLHWRIK 300

Query: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHQKVLE GVLDP+TTV+SIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHQKVLEGGVLDPKTTVISIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420
           TEKRDLYDADHGKKVLS+APGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGTK
Sbjct: 361 TEKRDLYDADHGKKVLSLAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420

Query: 421 MRNLARNKENPPDGFMCPSGWNVLVEYYKSLVPADNGRIPKPEAVAA 467
           MRNLARNKENPPDGFMCP GWNVLVEYY+SLVPADNGRIPKPEAVAA
Sbjct: 421 MRNLARNKENPPDGFMCPGGWNVLVEYYESLVPADNGRIPKPEAVAA 465

BLAST of Chy5G094230 vs. NCBI nr
Match: XP_023548667.1 (ATP sulfurylase 1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 874 bits (2257), Expect = 0.0
Identity = 426/467 (91.22%), Postives = 439/467 (94.00%), Query Frame = 0

Query: 1   MASMAAAFMKTSNLFPQSPITFTKAVTPPIMLPMSLRVKTKTHRRIRVSCGLIDPDGGKL 60
           MASMAAAF+K SN FP SPITF K  +P I LP+SLR+K    RRIRVSCGLIDPDGGKL
Sbjct: 1   MASMAAAFIKPSNAFPHSPITFVKTTSPWIQLPISLRIKNNARRRIRVSCGLIDPDGGKL 60

Query: 61  VELIVEESMRGSKNREALSLPRIKLSRIDLQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120
           V+LIV+ESMR  KNREALSLPR+KLSRIDLQWVHVLSEGWASPL+GFMRESEFLQTLHFN
Sbjct: 61  VDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHVLSEGWASPLSGFMRESEFLQTLHFN 120

Query: 121 SLRLQDGSVVNMSVPIVLAIDDDLKNRIGDSSMVALFGSDDRPVAILNDIEIYKHPKEER 180
           SLRL DGSVVNMSVPIVLAIDD +K RIG SS VALFGSDDRPVAILNDIEIYK PKEER
Sbjct: 121 SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRPVAILNDIEIYKQPKEER 180

Query: 181 IARTWGTTASGLPYVDEAITNAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAQLRNEFTSR 240
           IARTWGTTA GLPYVDEAI NAGNWLIGGDLEVIEPIKYHDGLD FRLSPAQLRNEF SR
Sbjct: 181 IARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFISR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLNMGYNNPILLLNPLGGYTKADDVPLHWRIK 300
           NADAVFAFQLRNPVHNGHALLMTDTRRRLL+MGY NPILLLNPLGGYTKADDVPLHWRIK
Sbjct: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRIK 300

Query: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420
           TEKRDLYD DHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGTK
Sbjct: 361 TEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420

Query: 421 MRNLARNKENPPDGFMCPSGWNVLVEYYKSLVPADNGRIPKPEAVAA 467
           MR LARNKENPPDGFMCP GW+VLVEYY+SLVPA+NGRIPKPEAVAA
Sbjct: 421 MRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANNGRIPKPEAVAA 467

BLAST of Chy5G094230 vs. NCBI nr
Match: KAG6574955.1 (ATP sulfurylase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 869 bits (2245), Expect = 0.0
Identity = 424/467 (90.79%), Postives = 437/467 (93.58%), Query Frame = 0

Query: 1   MASMAAAFMKTSNLFPQSPITFTKAVTPPIMLPMSLRVKTKTHRRIRVSCGLIDPDGGKL 60
           MASMAAAF+K SN FP SPITF K  +P I LP+SLR+K   HRRIRVSCGLIDPDGGKL
Sbjct: 1   MASMAAAFIKPSNAFPHSPITFIKTTSPSIKLPISLRIKNNAHRRIRVSCGLIDPDGGKL 60

Query: 61  VELIVEESMRGSKNREALSLPRIKLSRIDLQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120
           V+LIV+ESMR  KNREALSLPR+KLSRIDLQWVHVLSEGWASPL+GFMRESEFLQTLHFN
Sbjct: 61  VDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHVLSEGWASPLSGFMRESEFLQTLHFN 120

Query: 121 SLRLQDGSVVNMSVPIVLAIDDDLKNRIGDSSMVALFGSDDRPVAILNDIEIYKHPKEER 180
           SLRL DGSVVNMSVPIVLAIDD +K RIG SS VALFGSDDR VAILNDIEIYK PKEER
Sbjct: 121 SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRLVAILNDIEIYKQPKEER 180

Query: 181 IARTWGTTASGLPYVDEAITNAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAQLRNEFTSR 240
           IARTWGTTA GLPYVDEAI NAGNWLIGGDLEVIEPIKYHDGLD FRLSPAQLRNEF SR
Sbjct: 181 IARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFISR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLNMGYNNPILLLNPLGGYTKADDVPLHWRIK 300
           NADAVFAFQLRNPVHNGHALLM DTRRRLL+MGY NPILLLNPLGGYTKADDVPLHWRIK
Sbjct: 241 NADAVFAFQLRNPVHNGHALLMNDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRIK 300

Query: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420
           TEKRDLYD DHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGTK
Sbjct: 361 TEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420

Query: 421 MRNLARNKENPPDGFMCPSGWNVLVEYYKSLVPADNGRIPKPEAVAA 467
           MR LARNKENPPDGFMCP GW+VLVEYY+SLVPA+NG IPKPEAVAA
Sbjct: 421 MRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANNGSIPKPEAVAA 467

BLAST of Chy5G094230 vs. TAIR 10
Match: AT3G22890.1 (ATP sulfurylase 1 )

HSP 1 Score: 761.9 bits (1966), Expect = 3.0e-220
Identity = 370/461 (80.26%), Postives = 398/461 (86.33%), Query Frame = 0

Query: 1   MASMAAAFMKTSNLFPQSPITFTKAVTPPIMLPMSLRVKTKTHRRIRVSCGLIDPDGGKL 60
           MASMAA   KT   F   P+T +   +      +S   K+   R   +  GLI PDGGKL
Sbjct: 1   MASMAAVLSKTP--FLSQPLTKSSPNSDLPFAAVSFPSKSLRRRVGSIRAGLIAPDGGKL 60

Query: 61  VELIVEESMRGSKNREALSLPRIKLSRIDLQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120
           VELIVEE  R  K  EA  LPR++L+ IDLQW+HVLSEGWASPL GFMRESEFLQTLHFN
Sbjct: 61  VELIVEEPKRREKKHEAADLPRVELTAIDLQWMHVLSEGWASPLGGFMRESEFLQTLHFN 120

Query: 121 SLRLQDGSVVNMSVPIVLAIDDDLKNRIGDSSMVALFGSDDRPVAILNDIEIYKHPKEER 180
           SLRL DGSVVNMSVPIVLAIDD+ K RIG+S+ VALF SD  PVAIL+DIEIYKHPKEER
Sbjct: 121 SLRLDDGSVVNMSVPIVLAIDDEQKARIGESTRVALFNSDGNPVAILSDIEIYKHPKEER 180

Query: 181 IARTWGTTASGLPYVDEAITNAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAQLRNEFTSR 240
           IARTWGTTA GLPYVDEAITNAGNWLIGGDLEV+EP+KY+DGLDRFRLSPA+LR E   R
Sbjct: 181 IARTWGTTAPGLPYVDEAITNAGNWLIGGDLEVLEPVKYNDGLDRFRLSPAELRKELEKR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLNMGYNNPILLLNPLGGYTKADDVPLHWRIK 300
           NADAVFAFQLRNPVHNGHALLMTDTRRRLL MGY NPILLL+PLGG+TKADDVPL WR+K
Sbjct: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGFTKADDVPLDWRMK 300

Query: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QH+KVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420
            EKRDLYDADHGKKVLSMAPGLERLNILPF+VAAYDKTQGKMAFFDPSRPQDFLFISGTK
Sbjct: 361 VEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420

Query: 421 MRNLARNKENPPDGFMCPSGWNVLVEYYKSLVPADNGRIPK 462
           MR LA+N ENPPDGFMCP GW VLV+YY+SL PA NGR+P+
Sbjct: 421 MRTLAKNNENPPDGFMCPGGWKVLVDYYESLTPAGNGRLPE 459

BLAST of Chy5G094230 vs. TAIR 10
Match: AT4G14680.1 (Pseudouridine synthase/archaeosine transglycosylase-like family protein )

HSP 1 Score: 732.3 bits (1889), Expect = 2.5e-211
Identity = 357/464 (76.94%), Postives = 393/464 (84.70%), Query Frame = 0

Query: 1   MASMAAAFMKTSNLFPQSPIT---FTKAVTPPIMLPMSLRVKTKTHRRIRVSCGLIDPDG 60
           MASM+  F K ++   Q P+T    + +VT  I  P +   KT++ R I V  GLI+PDG
Sbjct: 1   MASMSTVFPKPTSFISQ-PLTKSHKSDSVTTSISFPSN--SKTRSLRTISVRAGLIEPDG 60

Query: 61  GKLVELIVEESMRGSKNREALSLPRIKLSRIDLQWVHVLSEGWASPLTGFMRESEFLQTL 120
           GKLV+L+V E  R  K  EA  LPR++L+ IDLQW+HVLSEGWASPL GFMRESEFLQTL
Sbjct: 61  GKLVDLVVPEPRRREKKHEAADLPRVRLTAIDLQWMHVLSEGWASPLRGFMRESEFLQTL 120

Query: 121 HFNSLRLQDGSVVNMSVPIVLAIDDDLKNRIGDSSMVALFGSDDRPVAILNDIEIYKHPK 180
           HFN L L DGSVVNMSVPIVLAIDD  K  IG+S  V+L  SDD P+AILNDIEIYKHPK
Sbjct: 121 HFNLLNLDDGSVVNMSVPIVLAIDDQQKALIGESKRVSLVDSDDNPIAILNDIEIYKHPK 180

Query: 181 EERIARTWGTTASGLPYVDEAITNAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAQLRNEF 240
           EERIARTWGTTA GLPYV+EAITNAG+WLIGGDLEV+EP+KY+DGLDRFRLSP +LR E 
Sbjct: 181 EERIARTWGTTAPGLPYVEEAITNAGDWLIGGDLEVLEPVKYNDGLDRFRLSPFELRKEL 240

Query: 241 TSRNADAVFAFQLRNPVHNGHALLMTDTRRRLLNMGYNNPILLLNPLGGYTKADDVPLHW 300
             R ADAVFAFQLRNPVHNGHALLMTDTRRRLL MGY NPILLL+PLGG+TKADDVPL W
Sbjct: 241 EKRGADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGFTKADDVPLSW 300

Query: 301 RIKQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGM 360
           R+KQH+KVLEDGVLDPETTVVSIFPSPM YAGPTEVQWHAKARINAGANFYIVGRDPAGM
Sbjct: 301 RMKQHEKVLEDGVLDPETTVVSIFPSPMLYAGPTEVQWHAKARINAGANFYIVGRDPAGM 360

Query: 361 GHPTEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFIS 420
           GHP EKRDLYDADHGKKVLSMAPGLERLNILPF+VAAYDKTQGKMAFFDPSR QDFLFIS
Sbjct: 361 GHPVEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFIS 420

Query: 421 GTKMRNLARNKENPPDGFMCPSGWNVLVEYYKSLVPADNGRIPK 462
           GTKMR LA+N+ENPPDGFMCP GW VLV+YY SL    N ++P+
Sbjct: 421 GTKMRALAKNRENPPDGFMCPGGWKVLVDYYDSLTLTGNTKLPE 461

BLAST of Chy5G094230 vs. TAIR 10
Match: AT5G43780.1 (Pseudouridine synthase/archaeosine transglycosylase-like family protein )

HSP 1 Score: 726.9 bits (1875), Expect = 1.1e-209
Identity = 362/471 (76.86%), Postives = 396/471 (84.08%), Query Frame = 0

Query: 1   MASMAAAFMKTSNLFPQSPITFTKAVT--PPIMLPMSLRVKTKTHRRIRVSCGLIDPDGG 60
           MAS AAA +  S  F  SP+      +   P  + +    +  + R + V  GLI+PDGG
Sbjct: 1   MASSAAAIVSGSP-FRSSPLIHNHHASRYAPGSISVVSLPRQVSRRGLSVKSGLIEPDGG 60

Query: 61  KLVELIVEESMRGSKNREALSLP-RIKLSRIDLQWVHVLSEGWASPLTGFMRESEFLQTL 120
           KL+ L+VEES R     EA ++P RIKL+R+DL+WVHVLSEGWASPL GFMR+SEFLQTL
Sbjct: 61  KLMNLVVEESRRRVMKHEAETVPARIKLNRVDLEWVHVLSEGWASPLKGFMRQSEFLQTL 120

Query: 121 HFNSLRLQDGSVVNMSVPIVLAIDDDLKNRIGDSSMVALFGSDDRPVAILNDIEIYKHPK 180
           HFNS RL+DGSVVNMSVPIVLAIDDD K RIGDS+ V L  S   P+AILNDIEIYKHPK
Sbjct: 121 HFNSFRLEDGSVVNMSVPIVLAIDDDQKFRIGDSNQVTLVDSVGNPIAILNDIEIYKHPK 180

Query: 181 EERIARTWGTTASGLPYVDEAITNAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAQLRNEF 240
           EERIARTWGTTA GLPY +EAIT AGNWLIGGDL+V+EPIKY+DGLDRFRLSP+QLR EF
Sbjct: 181 EERIARTWGTTARGLPYAEEAITKAGNWLIGGDLQVLEPIKYNDGLDRFRLSPSQLREEF 240

Query: 241 TSRNADAVFAFQLRNPVHNGHALLMTDTRRRLLNMGYNNPILLLNPLGGYTKADDVPLHW 300
             R ADAVFAFQLRNPVHNGHALLMTDTRRRLL MGY NP+LLLNPLGG+TKADDVPL W
Sbjct: 241 IRRGADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPVLLLNPLGGFTKADDVPLSW 300

Query: 301 RIKQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGM 360
           R++QH+KVLEDGVLDPETTVVSIFPSPM YAGPTEVQWHAKARINAGANFYIVGRDPAGM
Sbjct: 301 RMRQHEKVLEDGVLDPETTVVSIFPSPMLYAGPTEVQWHAKARINAGANFYIVGRDPAGM 360

Query: 361 GHPTEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFIS 420
           GHPTEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSR QDFLFIS
Sbjct: 361 GHPTEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRSQDFLFIS 420

Query: 421 GTKMRNLARNKENPPDGFMCPSGWNVLVEYYKSL-VPADNGRIPKPEAVAA 468
           GTKMR LA+ KENPPDGFMCPSGW VLV+YY SL     NGR+   EAVA+
Sbjct: 421 GTKMRGLAKKKENPPDGFMCPSGWKVLVDYYDSLSAETGNGRV--SEAVAS 468

BLAST of Chy5G094230 vs. TAIR 10
Match: AT1G19920.1 (Pseudouridine synthase/archaeosine transglycosylase-like family protein )

HSP 1 Score: 658.3 bits (1697), Expect = 4.6e-189
Identity = 308/407 (75.68%), Postives = 352/407 (86.49%), Query Frame = 0

Query: 45  RIRVSCGLIDPDGGKLVELIVEESMRGSKNREALSLPRIKLSRIDLQWVHVLSEGWASPL 104
           ++ V   LIDPDGG+LVELIV E+  G K  E+ ++P++KL++IDL+WVHV+SEGWASPL
Sbjct: 57  KMTVKSSLIDPDGGELVELIVPETEIGVKKAESETMPKVKLNQIDLEWVHVISEGWASPL 116

Query: 105 TGFMRESEFLQTLHFNSLRLQDGSVVNMSVPIVLAIDDDLKNRIGDSSMVALFGSDDRPV 164
            GFMRE E+LQ+LHFNSLRL++G+ VNMS+PIVLAIDDD K +IG S  VAL       +
Sbjct: 117 KGFMREDEYLQSLHFNSLRLKNGTFVNMSLPIVLAIDDDTKEQIGSSENVALVCPQGDII 176

Query: 165 AILNDIEIYKHPKEERIARTWGTTASGLPYVDEAITNAGNWLIGGDLEVIEPIKYHDGLD 224
             L  +EIYKH KEERIARTWGTT+ GLPYV+E IT +GNWLIGGDLEV EPIKY+DGLD
Sbjct: 177 GSLRSVEIYKHNKEERIARTWGTTSPGLPYVEEYITPSGNWLIGGDLEVFEPIKYNDGLD 236

Query: 225 RFRLSPAQLRNEFTSRNADAVFAFQLRNPVHNGHALLMTDTRRRLLNMGYNNPILLLNPL 284
            +RLSP QLR EF +R ADAVFAFQLRNPVHNGHALLM DTR+RLL MGY NP+LLL+PL
Sbjct: 237 HYRLSPKQLREEFDNRQADAVFAFQLRNPVHNGHALLMNDTRKRLLEMGYKNPVLLLHPL 296

Query: 285 GGYTKADDVPLHWRIKQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAG 344
           GG+TKADDVPL  R++QH KVLEDGVLDP+TT+VSIFPSPMHYAGPTEVQWHAKARINAG
Sbjct: 297 GGFTKADDVPLDVRMEQHSKVLEDGVLDPKTTIVSIFPSPMHYAGPTEVQWHAKARINAG 356

Query: 345 ANFYIVGRDPAGMGHPTEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAF 404
           ANFYIVGRDPAGMGHPTEKRDLYD DHGK+VLSMAPGLE+LNILPF+VAAYD  + KMAF
Sbjct: 357 ANFYIVGRDPAGMGHPTEKRDLYDPDHGKRVLSMAPGLEKLNILPFRVAAYDTIEKKMAF 416

Query: 405 FDPSRPQDFLFISGTKMRNLARNKENPPDGFMCPSGWNVLVEYYKSL 452
           FDPSR ++FLFISGTKMR  AR  ENPPDGFMCPSGWNVLV+YY+SL
Sbjct: 417 FDPSRAKEFLFISGTKMRTYARTGENPPDGFMCPSGWNVLVKYYESL 463

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LIK94.2e-21980.26ATP sulfurylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS1 PE=1 SV... [more]
O233243.6e-21076.94ATP-sulfurylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS3 PE=1 SV... [more]
Q9S7D81.5e-20876.86ATP sulfurylase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS4 PE=2 SV... [more]
Q438706.5e-18875.68ATP sulfurylase 2 OS=Arabidopsis thaliana OX=3702 GN=APS2 PE=1 SV=1[more]
O953401.3e-14060.61Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2 OS=Homo sapiens OX... [more]
Match NameE-valueIdentityDescription
A0A0A0LIM72.3e-27398.72Sulfate adenylyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G215520 PE=4 SV=1[more]
A0A1S3BMN34.7e-26696.57Sulfate adenylyltransferase OS=Cucumis melo OX=3656 GN=LOC103491225 PE=4 SV=1[more]
A0A6J1H3X22.8e-25090.79Sulfate adenylyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111459921 PE=4 S... [more]
A0A6J1L5Q78.1e-25089.91Sulfate adenylyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111499372 PE=4 SV=... [more]
A0A6J1CE702.7e-23786.78Sulfate adenylyltransferase OS=Momordica charantia OX=3673 GN=LOC111009889 PE=4 ... [more]
Match NameE-valueIdentityDescription
XP_004150566.10.098.72ATP sulfurylase 1, chloroplastic [Cucumis sativus] >KGN61658.1 hypothetical prot... [more]
XP_008449310.10.096.57PREDICTED: ATP sulfurylase 1, chloroplastic [Cucumis melo][more]
XP_038907157.10.092.93ATP sulfurylase 1, chloroplastic-like [Benincasa hispida][more]
XP_023548667.10.091.22ATP sulfurylase 1, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
KAG6574955.10.090.79ATP sulfurylase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia... [more]
Match NameE-valueIdentityDescription
AT3G22890.13.0e-22080.26ATP sulfurylase 1 [more]
AT4G14680.12.5e-21176.94Pseudouridine synthase/archaeosine transglycosylase-like family protein [more]
AT5G43780.11.1e-20976.86Pseudouridine synthase/archaeosine transglycosylase-like family protein [more]
AT1G19920.14.6e-18975.68Pseudouridine synthase/archaeosine transglycosylase-like family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR024951Sulphate adenylyltransferase catalytic domainPFAMPF01747ATP-sulfurylasecoord: 225..447
e-value: 2.9E-66
score: 223.0
NoneNo IPR availableGENE3D3.10.400.10Sulfate adenylyltransferasecoord: 51..219
e-value: 3.2E-57
score: 194.7
NoneNo IPR availablePANTHERPTHR11055:SF41ATP SULFURYLASE 1, CHLOROPLASTICcoord: 1..462
NoneNo IPR availablePANTHERPTHR11055BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHASEcoord: 1..462
NoneNo IPR availableSUPERFAMILY52374Nucleotidylyl transferasecoord: 225..448
IPR002650Sulphate adenylyltransferaseTIGRFAMTIGR00339TIGR00339coord: 55..446
e-value: 2.9E-137
score: 455.5
IPR002650Sulphate adenylyltransferaseCDDcd00517ATPScoord: 80..447
e-value: 3.02231E-164
score: 465.569
IPR025980ATP-sulfurylase PUA-like domainPFAMPF14306PUA_2coord: 53..215
e-value: 5.9E-44
score: 149.5
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 220..454
e-value: 9.6E-95
score: 318.3
IPR015947PUA-like superfamilySUPERFAMILY88697PUA domain-likecoord: 55..219

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy5G094230.1Chy5G094230.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
biological_process GO:0000103 sulfate assimilation
molecular_function GO:0004020 adenylylsulfate kinase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0004781 sulfate adenylyltransferase (ATP) activity