Chy5G094180 (gene) Cucumber (hystrix) v1

Overview


Name	Chy5G094180
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Pyruvate kinase
Location	chrH05: 4031421 .. 4035646 (-)
RNA-Seq Expression	Chy5G094180
Synteny	Chy5G094180

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACACAATCTCTCCAAATTTTCACTTCCTCCGCCATTTCTCTGCCCAAACACTCCATCTCTAAACCCTCCTCTTCCTCCGCCGCTTTCCGCTTTCCTTTCTCCTTCTCCAAATCCTCAATTAGAGCTTCTTCTTCTTCGGATCTCAATCCTCTCTCATCCTCTTCCACTTCCCAAGTCTTGGTTTCTGAAAATGGCTCCAGTTCCGGTGGTGGGGTTGTGTTCTCTTCTGCTGCTACTAGGGAGTTTGTGTCTGTTGCCTCTGATTCCACCTCGATTGAGGTTGATGCTGTCACTGAGGCTGAGTTGAAGGAGAATGGGTTCAGGAGTACCAGGAGGACTAAGCTCGTGTGCACTATTGGCCCTGCTACTTGTGGATTCGAACAGCTCGAGGCGCTCGCTGTTGGTGGTATGAATGTCGCTAGGATCAATATGTGCCATGGGACTCGAGACTGGCATCGAACCGTTATTGAACGTGTGCGGAGGCTCAATGATGAGAAGGGATATGCTGTTGCTATTATGATGGACACCGAAGGGAGTGAAATTCACATGGGTGATCTTGGTGGAGCCTCCTCGGCTAAAGCGGAAGTGAGTTCTTCGATTCTTTTACCGTTTTTTTAATCTTATCGTCTCATAATCAGTCGAATTCTTGATAATCTTTGCCCCAGGTAAAAAGTTTTCTGATTGCATTGGGATACTGGGTTTTGTCTAGCTGAGAAACGTTTCTTCTGCCTGAAACTCTATAATCTGCTAGATTTCTAGATTCTTAAATTGGGTCATAGTTCACTTTCCTTGAAACCTAGTTTTCATAAAGATATTACCCATTTTGTGTTTTAAAACGAAGATGGGTCCTTTCATCATTAATCAAGTCGTGAACTTTTGTCAAGGTTCTTAAATATGTTGCTATCATAACGTACGTGTCACGATATCTTGGCTCAATGCATAAACTTTGATCACTGGATTAGTGAAAATGAGTTTACTTACTGTGCAGCTGTAAGTATTCTCGAAGTGGTACCCAGTGAGTCAGTGTTTTATAAAGCTCTCCCGGCACATGCCTAAGCGTTAGGCGTAAGTCTGGCGCTTCTTCTTGGAAAGGCAAGCTTACGAAATTAGGCGCGTCTTCTATGAAGTCCCAAGGTTAAAAGGTTCAAAGCCCTCGGTCTTGGAACATTTTCATTATTTTTATAATATAGTAATAATGGGGGGTTCTTTTTTTCTTTAACTATAAAAAACTAAACTTACTAATGTTACGTGCAAAATTTCTTGTGCGAAGGGATATTTTTTTCTTCCAACTTTCCACTTAATACTCAAAAAGTGATATATTGCAACTTGAGCTTGTCAACATTTGCGATACTTATGAATTAGCATAGTTCTTCTAATTATAAGCAAGGAAGATGGTCCTTCATTTCTGTTATGTTACTGCTATCTGACTCCGGTTTACATAGGGCGATGGGGAAGTCGTTGGGTGGGACATGTTTAACTGCTTGCTCTGTTCTATGTAGGATGGTGAAATATGGACATTCAGTGTCAGAGCTTTTGATTCAACCCTCCCAGAACGCACCATTAATGTGAACTATGAAGGGTTTGCAGAAGGTTACTGTTTCTATCCTAAATAAAAAATTCGCTTTGGCATTTACTGAATGCATGCACAATTTAATACAGCCTTTGGAAATGGCGTGTGTGGATTGGATGTATCTGTGTTCATTGTTATAGTAAGTTCCATGATATTGTTTTTGATCTGGAATTATCATTGATGTTGTGTAGATGTGAGAGTGGGTGATGACCTTCTTGTTGATGGTGGAATGGTGAGGTTTGAGGTAATCGAAAAAATTGGTCCAGATGTTAAATGCCTGTGTACTGACCCGGGATTGTTGTTGCCACGGGCTAATTTGACTTTTTGGAGGGATGGACATCTAGTACGAGAACGTAATGCCATGCTCCCTACAATTTCTTCTAAGGTAGGTGCCTCTCCTTCCTTAAAATGTCATTTCATTGGTTAAATTGAATTCATTTGATATACAATACCTAATAAAGTACCGATATACCATTAAATTGAACGTAACTAGTCAACTTAAGCTTTTGGATGCATCATTTAATTTTGTGTGATTTTTGAGTTGGGTGTTATGTGCTCGAATCTCTTTAATGTCATTTCTTTCCCGACTGATGACCAACTTGGGTTTTTTGCCAATATCTCTAACTCTGCAAGTACAGGGTTAAAGTATTGTTATAATATGAAAATTTCCATAATTTATGAGCTTAAGATCTTAGGTTAAGTAGTGATTTTACAATTACTAATAATTGTTGTTATGACTGATTGTGGAAAGAGTTTCAGACTGCGACAAGAGTTCCATCATCTAGGCTTGCCATATTTTTTTGAACCTGTGTAATATGTATAGTTAATTTTAGATCACGTTGTTTTAGAACTTGCAGACTTTATACTATTTTATTTTTTTAAGATATGTTATTGTCAGAAACTTTACCCTAGAATTAGAAAGGTTTTGCTCTCTGATCAGAATCTAACACTTTTGTTTAATTAGCTTAGATATTCAAACTTTCGATTTTGGCATTGCAACCAATTCACACTACATTTTGTAGAGTGACTAGATTTATGTTGAAAGATCATGTAGAGATAATTATAATTTGGACCATAAATCTTAGAACATTTTTCCTGGTGTGAGTAGTTGTATATTCACACTGCGTACTCTTCATGCAACCTAATGATTGCCTCTGTTCCTGGATCAAAGCCTCCTCATATTCTAGGTTAATCTTTTGTGCAACCTTATGGCACAAATTTCCCTTTTACAGTGGCTTCTTTTCTTGTCCGAATTTCTAATGCAAGTTTTACAAATCCAGGATTGGTTGGATATTGATTTTGGGATTGCTGAAGGTGTTGATTTTCTTGCCATATCATTTGTCAAGTCTGCTGAAGTAATTAAACATCTCAAAAGCTATATCGCTGCACGTTCTCGTGGCAGGTGAGCAGAATTATGGTATTGTGCTGATTTGCTCTTTTACTCCATTTGGGTCTGAAGCTTAGGACCTTTCATTGTTTGTTCTCACGGATTTAGAGGGACCAAATGTTTCTCAACTATGTGTTAGTATGTTTTGATTCATAAGCTAGGTATTGACAGGCTAGTTGATGGCTGTCACATTTTCCCTCCAATTCTGTAAATTTCATCCCTAAAATGTTCATATGTGCGCCTCCTAAATATTTGTGTTTCTAATTTCACATGCAGTGACATTTCCATCATTGCAAAAATAGAGAGTCTGGACTCGTTGAAAAACTTGGAAGAAATTATTTTAGCATCAGATGGAGCTATGGTAGCTAGAGGAGATTTAGGAGCTCAAATACCACTGGAACAGGTGCCATCAGTCCAGCAAAAGGTCGTCCAACTGTGCAGACAATTAAATAAACCAGTTATTGTTGCTTCTCAGCTGCTTGAGTCAATGATCGAATATCCTACACCCACCAGAGCTGAAGTGGCTGATGTTTCCGAGGCGGTTAGGCAGCGATCAGATGCTCTAATGCTGTCTGGTGAGTCCGCCATGGGCCAGTACCCAGACAAGGCATTGGCTGTTTTGAGAAGTGTCAGTCTAAGAATTGAAAAGTGGTGGAGGGATGAGAAACGCCATGAACCTATGGAACTCCCAGAAGTTGGATTTTCATTCTCAGATAGTATCTTAGAAGAGATCTGCAATTCGGCTGCTAAGATGGGTAAGCCTAAGTTTTATGATTCCTTTGTCCTGTTGTAGATAGTGTACCTTTAGAACCATAATTTAGATCTATGTTTTAGTACCATGAACTCAAATAACCTCATATCATTTCACAAAAGTGGTCATTAGGCTGTATAACAATGGTTTCTTGAGTTCTGTTCCTCTTTGTTTACTACGAATCCAGTCTAATTGTGAATTTTGTTTCTCTAGCTAATAATTTGGAGGTAGATGCGATTTTTGTTTACACAACGTCAGGCCACATGGCATCTCTGCTGTCCCGTTGCCGACCTGATTGCCCAATCTTTGCTTTTACTTCCACAACATCTGTTAGGAGACGTCTTAACTTGCAATGGGGATTGATACCCTTCCGACTGAGCTTCTCCGACGACATGGAAAACAACCTCAACAAAACATTTTTGCTGCTTAAAGCCAGAAACTTGATCAAATCAGGTGATCTTGTAATTGCTGTCTCAGACATGTTACAATCTATCCAAGTTATGAATGTTCCATAA

mRNA sequence

ATGACACAATCTCTCCAAATTTTCACTTCCTCCGCCATTTCTCTGCCCAAACACTCCATCTCTAAACCCTCCTCTTCCTCCGCCGCTTTCCGCTTTCCTTTCTCCTTCTCCAAATCCTCAATTAGAGCTTCTTCTTCTTCGGATCTCAATCCTCTCTCATCCTCTTCCACTTCCCAAGTCTTGGTTTCTGAAAATGGCTCCAGTTCCGGTGGTGGGGTTGTGTTCTCTTCTGCTGCTACTAGGGAGTTTGTGTCTGTTGCCTCTGATTCCACCTCGATTGAGGTTGATGCTGTCACTGAGGCTGAGTTGAAGGAGAATGGGTTCAGGAGTACCAGGAGGACTAAGCTCGTGTGCACTATTGGCCCTGCTACTTGTGGATTCGAACAGCTCGAGGCGCTCGCTGTTGGTGGTATGAATGTCGCTAGGATCAATATGTGCCATGGGACTCGAGACTGGCATCGAACCGTTATTGAACGTGTGCGGAGGCTCAATGATGAGAAGGGATATGCTGTTGCTATTATGATGGACACCGAAGGGAGTGAAATTCACATGGGTGATCTTGGTGGAGCCTCCTCGGCTAAAGCGGAAGATGGTGAAATATGGACATTCAGTGTCAGAGCTTTTGATTCAACCCTCCCAGAACGCACCATTAATGTGAACTATGAAGGGTTTGCAGAAGATGTGAGAGTGGGTGATGACCTTCTTGTTGATGGTGGAATGGTGAGGTTTGAGGTAATCGAAAAAATTGGTCCAGATGTTAAATGCCTGTGTACTGACCCGGGATTGTTGTTGCCACGGGCTAATTTGACTTTTTGGAGGGATGGACATCTAGTACGAGAACGTAATGCCATGCTCCCTACAATTTCTTCTAAGGATTGGTTGGATATTGATTTTGGGATTGCTGAAGGTGTTGATTTTCTTGCCATATCATTTGTCAAGTCTGCTGAAGTAATTAAACATCTCAAAAGCTATATCGCTGCACGTTCTCGTGGCAGTGACATTTCCATCATTGCAAAAATAGAGAGTCTGGACTCGTTGAAAAACTTGGAAGAAATTATTTTAGCATCAGATGGAGCTATGGTAGCTAGAGGAGATTTAGGAGCTCAAATACCACTGGAACAGGTGCCATCAGTCCAGCAAAAGGTCGTCCAACTGTGCAGACAATTAAATAAACCAGTTATTGTTGCTTCTCAGCTGCTTGAGTCAATGATCGAATATCCTACACCCACCAGAGCTGAAGTGGCTGATGTTTCCGAGGCGGTTAGGCAGCGATCAGATGCTCTAATGCTGTCTGGTGAGTCCGCCATGGGCCAGTACCCAGACAAGGCATTGGCTGTTTTGAGAAGTGTCAGTCTAAGAATTGAAAAGTGGTGGAGGGATGAGAAACGCCATGAACCTATGGAACTCCCAGAAGTTGGATTTTCATTCTCAGATAGTATCTTAGAAGAGATCTGCAATTCGGCTGCTAAGATGGCTAATAATTTGGAGGTAGATGCGATTTTTGTTTACACAACGTCAGGCCACATGGCATCTCTGCTGTCCCGTTGCCGACCTGATTGCCCAATCTTTGCTTTTACTTCCACAACATCTGTTAGGAGACGTCTTAACTTGCAATGGGGATTGATACCCTTCCGACTGAGCTTCTCCGACGACATGGAAAACAACCTCAACAAAACATTTTTGCTGCTTAAAGCCAGAAACTTGATCAAATCAGGTGATCTTGTAATTGCTGTCTCAGACATGTTACAATCTATCCAAGTTATGAATGTTCCATAA

Coding sequence (CDS)

ATGACACAATCTCTCCAAATTTTCACTTCCTCCGCCATTTCTCTGCCCAAACACTCCATCTCTAAACCCTCCTCTTCCTCCGCCGCTTTCCGCTTTCCTTTCTCCTTCTCCAAATCCTCAATTAGAGCTTCTTCTTCTTCGGATCTCAATCCTCTCTCATCCTCTTCCACTTCCCAAGTCTTGGTTTCTGAAAATGGCTCCAGTTCCGGTGGTGGGGTTGTGTTCTCTTCTGCTGCTACTAGGGAGTTTGTGTCTGTTGCCTCTGATTCCACCTCGATTGAGGTTGATGCTGTCACTGAGGCTGAGTTGAAGGAGAATGGGTTCAGGAGTACCAGGAGGACTAAGCTCGTGTGCACTATTGGCCCTGCTACTTGTGGATTCGAACAGCTCGAGGCGCTCGCTGTTGGTGGTATGAATGTCGCTAGGATCAATATGTGCCATGGGACTCGAGACTGGCATCGAACCGTTATTGAACGTGTGCGGAGGCTCAATGATGAGAAGGGATATGCTGTTGCTATTATGATGGACACCGAAGGGAGTGAAATTCACATGGGTGATCTTGGTGGAGCCTCCTCGGCTAAAGCGGAAGATGGTGAAATATGGACATTCAGTGTCAGAGCTTTTGATTCAACCCTCCCAGAACGCACCATTAATGTGAACTATGAAGGGTTTGCAGAAGATGTGAGAGTGGGTGATGACCTTCTTGTTGATGGTGGAATGGTGAGGTTTGAGGTAATCGAAAAAATTGGTCCAGATGTTAAATGCCTGTGTACTGACCCGGGATTGTTGTTGCCACGGGCTAATTTGACTTTTTGGAGGGATGGACATCTAGTACGAGAACGTAATGCCATGCTCCCTACAATTTCTTCTAAGGATTGGTTGGATATTGATTTTGGGATTGCTGAAGGTGTTGATTTTCTTGCCATATCATTTGTCAAGTCTGCTGAAGTAATTAAACATCTCAAAAGCTATATCGCTGCACGTTCTCGTGGCAGTGACATTTCCATCATTGCAAAAATAGAGAGTCTGGACTCGTTGAAAAACTTGGAAGAAATTATTTTAGCATCAGATGGAGCTATGGTAGCTAGAGGAGATTTAGGAGCTCAAATACCACTGGAACAGGTGCCATCAGTCCAGCAAAAGGTCGTCCAACTGTGCAGACAATTAAATAAACCAGTTATTGTTGCTTCTCAGCTGCTTGAGTCAATGATCGAATATCCTACACCCACCAGAGCTGAAGTGGCTGATGTTTCCGAGGCGGTTAGGCAGCGATCAGATGCTCTAATGCTGTCTGGTGAGTCCGCCATGGGCCAGTACCCAGACAAGGCATTGGCTGTTTTGAGAAGTGTCAGTCTAAGAATTGAAAAGTGGTGGAGGGATGAGAAACGCCATGAACCTATGGAACTCCCAGAAGTTGGATTTTCATTCTCAGATAGTATCTTAGAAGAGATCTGCAATTCGGCTGCTAAGATGGCTAATAATTTGGAGGTAGATGCGATTTTTGTTTACACAACGTCAGGCCACATGGCATCTCTGCTGTCCCGTTGCCGACCTGATTGCCCAATCTTTGCTTTTACTTCCACAACATCTGTTAGGAGACGTCTTAACTTGCAATGGGGATTGATACCCTTCCGACTGAGCTTCTCCGACGACATGGAAAACAACCTCAACAAAACATTTTTGCTGCTTAAAGCCAGAAACTTGATCAAATCAGGTGATCTTGTAATTGCTGTCTCAGACATGTTACAATCTATCCAAGTTATGAATGTTCCATAA

Protein sequence

MTQSLQIFTSSAISLPKHSISKPSSSSAAFRFPFSFSKSSIRASSSSDLNPLSSSSTSQVLVSENGSSSGGGVVFSSAATREFVSVASDSTSIEVDAVTEAELKENGFRSTRRTKLVCTIGPATCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGFSFSDSILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP*

Homology

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BLAST of Chy5G094180 vs. ExPASy Swiss-Prot
Match: Q9LIK0 (Plastidial pyruvate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PKP1 PE=1 SV=1)

HSP 1 Score: 922.5 bits (2383), Expect = 2.3e-267
Identity = 482/600 (80.33%), Postives = 533/600 (88.83%), Query Frame = 0


Query: 1   MTQSLQIFTSS----AISLPKHSISKPSSSSAAFRFPFSFSK-SSIRASSSS----DLNP 60
           M+QS+Q  T S     + LP    ++P SS +  RFP +  K +SIRASSSS    DL+ 
Sbjct: 1   MSQSIQFSTPSHTPHLLHLPHSQFNRPLSSISFRRFPLTTIKYTSIRASSSSSPSPDLDS 60

Query: 61  LSSSSTSQVLVSENGSSSGGGVVFSSAATREFVSVASDSTSIEVDAVTEAELKENGFRST 120
            SSSS+SQVL+S NG+    G V S   +    +V +D++ IEVD VTEAELKENGFRST
Sbjct: 61  SSSSSSSQVLLSPNGT----GAVKSDERSVVATAVTTDTSGIEVDTVTEAELKENGFRST 120

Query: 121 RRTKLVCTIGPATCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAV 180
           RRTKL+CTIGPATCGFEQLEALAVGGMNVAR+NMCHGTRDWHR VI  VRRLN+EKG+AV
Sbjct: 121 RRTKLICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDWHRGVIRSVRRLNEEKGFAV 180

Query: 181 AIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVG 240
           AIMMDTEGSEIHMGDLGG +SAKAEDGE+WTF+VRAFDS+ PERTI+V+Y+GFAEDVRVG
Sbjct: 181 AIMMDTEGSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSSRPERTISVSYDGFAEDVRVG 240

Query: 241 DDLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSK 300
           D+LLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDG LVRERNAMLPTISSK
Sbjct: 241 DELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGSLVRERNAMLPTISSK 300

Query: 301 DWLDIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEE 360
           DWLDIDFGIAEGVDF+A+SFVKSAEVI HLKSY+AARSRG +I +IAKIES+DSL NLEE
Sbjct: 301 DWLDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRGGEIGVIAKIESIDSLTNLEE 360

Query: 361 IILASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTR 420
           IILASDGAMVARGDLGAQIPLEQVP+ QQ++VQ+CR LNKPVIVASQLLESMIEYPTPTR
Sbjct: 361 IILASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNKPVIVASQLLESMIEYPTPTR 420

Query: 421 AEVADVSEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPE 480
           AEVADVSEAVRQRSDALMLSGESAMGQ+PDKAL VLR+VSLRIE+WWR+EKRHE + L  
Sbjct: 421 AEVADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVSLRIERWWREEKRHESVPLQA 480

Query: 481 VGFSFSDSILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSV 540
           +G SFSD I EEICNSAAKMANNL VDA+FVYTTSGHMASL+SRCRPDCPIFAFT+TTSV
Sbjct: 481 IGSSFSDKISEEICNSAAKMANNLGVDAVFVYTTSGHMASLVSRCRPDCPIFAFTTTTSV 540

Query: 541 RRRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 592
           RRRLNLQWGLIPFRLSFSDDME+NLNKTF LLK+R +IKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 RRRLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSRGMIKSGDLVIAVSDMLQSIQVMNVP 596

[Close]

BLAST of Chy5G094180 vs. ExPASy Swiss-Prot
Match: Q43117 (Pyruvate kinase isozyme A, chloroplastic OS=Ricinus communis OX=3988 PE=1 SV=1)

HSP 1 Score: 918.3 bits (2372), Expect = 4.4e-266
Identity = 487/595 (81.85%), Postives = 535/595 (89.92%), Query Frame = 0


Query: 1   MTQSLQI---FTSSAISLPKHSISKPSSSSAAFRFPFSFSKS-SIRASSSSDLNPLSSSS 60
           M+QSL      T +    PK  +  P+S+S   R+P +  KS SI+AS+S      SSSS
Sbjct: 1   MSQSLHFSPNLTFAKQPFPKLPLPFPTSNS---RYPVNNYKSLSIKASTSP-----SSSS 60

Query: 61  TSQVLVSENGSSSGGGVVFSSAATREFVSVASDSTSIEVDAVTEAELKENGFRSTRRTKL 120
             QVLV++NG+ +  GV++++    + V+V SD +SIEVDAVTE ELKENGFRSTRRTKL
Sbjct: 61  DPQVLVADNGTGN-SGVLYNN--NNKSVTV-SDPSSIEVDAVTETELKENGFRSTRRTKL 120

Query: 121 VCTIGPATCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMD 180
           VCTIGPATCGFE+LEALAVGGMNVARINMCHGTR+WH++VIERVRRLN+EKG+AVAIMMD
Sbjct: 121 VCTIGPATCGFEELEALAVGGMNVARINMCHGTREWHKSVIERVRRLNEEKGFAVAIMMD 180

Query: 181 TEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLV 240
           TEGSEIHMGDLGGASSAKAEDGEIWTFSVRA+DS  PERTINVNY+GFAEDV+VGD+LLV
Sbjct: 181 TEGSEIHMGDLGGASSAKAEDGEIWTFSVRAYDSPRPERTINVNYDGFAEDVKVGDELLV 240

Query: 241 DGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDI 300
           DGGMVRFEVIEKIGPDVKC CTDPGLLLPRANLTFWRDG LVRERNAMLPTISSKDWLDI
Sbjct: 241 DGGMVRFEVIEKIGPDVKCRCTDPGLLLPRANLTFWRDGSLVRERNAMLPTISSKDWLDI 300

Query: 301 DFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILAS 360
           DFGIAEGVDF+AISFVKSAEVI HLKSYIAARSR SDI++IAKIES+DSLKNLEEII AS
Sbjct: 301 DFGIAEGVDFIAISFVKSAEVINHLKSYIAARSRDSDIAVIAKIESIDSLKNLEEIIRAS 360

Query: 361 DGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVAD 420
           DGAMVARGDLGAQIPLEQVPS QQ +VQ+CRQLNKPVIVASQLLESMIEYPTPTRAEVAD
Sbjct: 361 DGAMVARGDLGAQIPLEQVPSAQQNIVQVCRQLNKPVIVASQLLESMIEYPTPTRAEVAD 420

Query: 421 VSEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGFSF 480
           VSEAVRQR+DALMLSGESAMGQYP+KALAVLRSVS+RIEKWWR+EK HE MELP +G ++
Sbjct: 421 VSEAVRQRADALMLSGESAMGQYPEKALAVLRSVSVRIEKWWREEKHHEAMELPAIGSTY 480

Query: 481 SDSILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLN 540
           SDSI EEICNSAAKMANNL VDA+FVYT  GHMASLLSRCRPDCPIFAFT+TTSVRRRLN
Sbjct: 481 SDSISEEICNSAAKMANNLGVDALFVYTKDGHMASLLSRCRPDCPIFAFTTTTSVRRRLN 540

Query: 541 LQWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 592
           LQWGLIPFRLSF+DDME+NLNKTF LLKAR +IKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 LQWGLIPFRLSFADDMESNLNKTFSLLKARGMIKSGDLVIAVSDMLQSIQVMNVP 583

[Close]

BLAST of Chy5G094180 vs. ExPASy Swiss-Prot
Match: Q40545 (Pyruvate kinase isozyme A, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 907.5 bits (2344), Expect = 7.8e-263
Identity = 477/598 (79.77%), Postives = 531/598 (88.80%), Query Frame = 0


Query: 1   MTQSLQIFTSSAISLP-KHSISKPS--SSSAAFRFPFSFS-KSSIRASSSSDLNPLSSSS 60
           M+Q+L  F SS+   P   +IS+PS   S+ + R     S ++ +  +SS+  + L    
Sbjct: 1   MSQALNFFVSSSSRSPATFTISRPSVFPSTGSLRLLVKKSLRTLVVEASSAAASDLDEPQ 60

Query: 61  TSQVLVSENGSSSGGGVVFSSAATREF---VSVASDSTSIEVDAVTEAELKENGFRSTRR 120
           +S VLVSENGS   GGV+  S+AT+E+    +  +DS+SIEVD VTEAELKENGFRSTRR
Sbjct: 61  SSPVLVSENGS---GGVL--SSATQEYGRNAAPGTDSSSIEVDTVTEAELKENGFRSTRR 120

Query: 121 TKLVCTIGPATCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAI 180
           TKL+CTIGPATCGFEQLE LA GGMNVARINMCHGTR+WHR VIER+RRLN+EKG+AVAI
Sbjct: 121 TKLICTIGPATCGFEQLERLAEGGMNVARINMCHGTREWHRMVIERLRRLNEEKGFAVAI 180

Query: 181 MMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDD 240
           MMDTEGSEIHMGDLGGASSAKAEDGEIW F+VR+FD  LPERT+ VNY+GFAEDV+VGD+
Sbjct: 181 MMDTEGSEIHMGDLGGASSAKAEDGEIWNFTVRSFDPPLPERTVTVNYDGFAEDVKVGDE 240

Query: 241 LLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDW 300
           LLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDG LVRERNAMLPTISSKDW
Sbjct: 241 LLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGKLVRERNAMLPTISSKDW 300

Query: 301 LDIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEII 360
           LDIDFGIAEGVDF+A+SFVKSAEVIKHLKSYI AR+R SDIS+IAKIES+DSLKNLEEII
Sbjct: 301 LDIDFGIAEGVDFIAVSFVKSAEVIKHLKSYIQARARDSDISVIAKIESIDSLKNLEEII 360

Query: 361 LASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAE 420
            ASDGAMVARGDLGAQIPLEQVPS QQK+VQ+CRQLN+PVIVASQLLESMIEYP PTRAE
Sbjct: 361 QASDGAMVARGDLGAQIPLEQVPSEQQKIVQICRQLNRPVIVASQLLESMIEYPIPTRAE 420

Query: 421 VADVSEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVG 480
           VADVSEAVRQR DALMLSGESAMGQ+P+KAL VLRSVSLRIE+ WR++KRHE +ELP + 
Sbjct: 421 VADVSEAVRQRGDALMLSGESAMGQFPEKALTVLRSVSLRIERMWREQKRHEVIELPSIA 480

Query: 481 FSFSDSILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRR 540
            SFSDSI EEICNSAAKMANNLEVDA+FVYT +GHMASLLSRCRPDCPIFAFT+TTSVRR
Sbjct: 481 SSFSDSISEEICNSAAKMANNLEVDALFVYTKNGHMASLLSRCRPDCPIFAFTTTTSVRR 540

Query: 541 RLNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 592
           RLNLQWGL+PFRLSFSDDME+NLNKTF LLKAR +IKSGDL+IAVSDMLQSIQVMNVP
Sbjct: 541 RLNLQWGLMPFRLSFSDDMESNLNKTFSLLKARGMIKSGDLIIAVSDMLQSIQVMNVP 593

[Close]

BLAST of Chy5G094180 vs. ExPASy Swiss-Prot
Match: Q40546 (Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 1.7e-100
Identity = 210/477 (44.03%), Postives = 296/477 (62.05%), Query Frame = 0


Query: 110 STRRTKLVCTIGPATCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDE-KG 169
           S R+TK+VCTIGP+T   E +  LA  GMNVAR+NM HG    H+  I+ V+  N + + 
Sbjct: 89  SPRKTKIVCTIGPSTSSREMIWKLAEAGMNVARLNMSHGDHASHQRTIDLVKEYNAQFED 148

Query: 170 YAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDV 229
             +AIM+DT+G E+  GD+        ++G+ + FS++   ST  E T++VNY+ F  DV
Sbjct: 149 KVIAIMLDTKGPEVISGDV--PKPILLKEGQEFNFSIKRGVST--EDTVSVNYDDFINDV 208

Query: 230 RVGDDLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTI 289
             GD LLVDGGM+   V  K    VKC   D G L  R +L        VR ++A LP+I
Sbjct: 209 EAGDILLVDGGMMSLAVKSKTSDIVKCEVIDGGELKSRRHLN-------VRGKSATLPSI 268

Query: 290 SSKDWLDIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKN 349
           + KDW DI FG+   VDF A+SFVK A+V+  LK Y+  +S  +DI +I KIES DS+ N
Sbjct: 269 TEKDWDDIKFGVNNQVDFYAVSFVKDAKVVHELKDYL--KSCNADIHVIVKIESADSIPN 328

Query: 350 LEEIILASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPT 409
           L  II ASDGAMVARGDLGA++P+E+VP +Q+ +++ C+ + KPVIVA+ +LESMI++PT
Sbjct: 329 LHSIISASDGAMVARGDLGAELPIEEVPLLQEDIIRRCQSMQKPVIVATNMLESMIDHPT 388

Query: 410 PTRAEVADVSEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPME 469
           PTRAEV+D+S AVR+ +DA+MLSGE+A G+YP KA+ V+  V+LR E         +   
Sbjct: 389 PTRAEVSDISIAVREGADAVMLSGETAHGKYPLKAVKVMHIVALRTE-----SSLQKSTS 448

Query: 470 LPEVGFSFSDSILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTST 529
            P    ++   + E     ++ MAN L    I V+T +G MA +LS  RP   +FAFT+ 
Sbjct: 449 SPSQSAAYKSHMGEMFAFHSSSMANTLST-PIIVFTRTGSMAIILSHNRPSSTVFAFTNN 508

Query: 530 TSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSI 586
             V++RL L  G++P  + FS D E   ++   LL +++L+K G  V  V    Q I
Sbjct: 509 ERVKQRLALYHGVVPIYMEFSSDAEETFSRAIKLLLSKSLVKDGQYVTLVQSGAQPI 546

[Close]

BLAST of Chy5G094180 vs. ExPASy Swiss-Prot
Match: Q9FLW9 (Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 3.2e-99
Identity = 207/475 (43.58%), Postives = 292/475 (61.47%), Query Frame = 0


Query: 112 RRTKLVCTIGPATCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDE-KGYA 171
           R+TK+VCT+GP+T   E +  LA  GMNVAR+NM HG    H+ VI+ V+  N + K   
Sbjct: 110 RKTKIVCTVGPSTNTREMIWKLAEAGMNVARMNMSHGDHASHKKVIDLVKEYNAQTKDNT 169

Query: 172 VAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRV 231
           +AIM+DT+G E+  GDL        + G+ +TF++    ST     ++VNY+ F  DV  
Sbjct: 170 IAIMLDTKGPEVRSGDL--PQPIMLDPGQEFTFTIERGVST--PSCVSVNYDDFVNDVEA 229

Query: 232 GDDLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISS 291
           GD LLVDGGM+ F V  K    VKC   D G L  R +L        VR ++A LP+I+ 
Sbjct: 230 GDMLLVDGGMMSFMVKSKTKDSVKCEVVDGGELKSRRHLN-------VRGKSATLPSITE 289

Query: 292 KDWLDIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLE 351
           KDW DI FG+   VDF A+SFVK A+V+  LK Y+  ++ G+DI +I KIES DS+ NL 
Sbjct: 290 KDWEDIKFGVENKVDFYAVSFVKDAQVVHELKKYL--QNSGADIHVIVKIESADSIPNLH 349

Query: 352 EIILASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPT 411
            II ASDGAMVARGDLGA++P+E+VP +Q++++ LCR + K VIVA+ +LESMI +PTPT
Sbjct: 350 SIITASDGAMVARGDLGAELPIEEVPILQEEIINLCRSMGKAVIVATNMLESMIVHPTPT 409

Query: 412 RAEVADVSEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELP 471
           RAEV+D++ AVR+ +DA+MLSGE+A G++P KA  V+ +V+LR E      +       P
Sbjct: 410 RAEVSDIAIAVREGADAVMLSGETAHGKFPLKAAGVMHTVALRTEATITSGEMP-----P 469

Query: 472 EVGFSFSDSILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTS 531
            +G +F + + E     A  M+N L    + V+T +G MA LLS  RP   I+AFT+   
Sbjct: 470 NLGQAFKNHMSEMFAYHATMMSNTLGTSTV-VFTRTGFMAILLSHYRPSGTIYAFTNEKK 529

Query: 532 VRRRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSI 586
           +++RL L  G+ P  + F+DD E         L  + ++K G+ +  V    Q I
Sbjct: 530 IQQRLALYQGVCPIYMEFTDDAEETFANALATLLKQGMVKKGEEIAIVQSGTQPI 565

[Close]

BLAST of Chy5G094180 vs. ExPASy TrEMBL
Match: A0A0A0LKS3 (Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_2G215480 PE=3 SV=1)

HSP 1 Score: 1125.9 bits (2911), Expect = 0.0e+00
Identity = 588/591 (99.49%), Postives = 589/591 (99.66%), Query Frame = 0


Query: 1   MTQSLQIFTSSAISLPKHSISKPSSSSAAFRFPFSFSKSSIRASSSSDLNPLSSSSTSQV 60
           MTQSLQ+FTSSAISLPKHSISKPSSSSAAFRFPFSFSKSSIRASSSSDLNPLSSSSTSQV
Sbjct: 1   MTQSLQLFTSSAISLPKHSISKPSSSSAAFRFPFSFSKSSIRASSSSDLNPLSSSSTSQV 60

Query: 61  LVSENGSSSGGGVVFSSAATREFVSVASDSTSIEVDAVTEAELKENGFRSTRRTKLVCTI 120
           LVSENGSSSGGGVVFSSAATREFVSVASDSTSIEVDAVTEAELKENGFRSTRRTKLVCTI
Sbjct: 61  LVSENGSSSGGGVVFSSAATREFVSVASDSTSIEVDAVTEAELKENGFRSTRRTKLVCTI 120

Query: 121 GPATCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMDTEGS 180
           GPATCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLND KGYAVAIMMDTEGS
Sbjct: 121 GPATCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDGKGYAVAIMMDTEGS 180

Query: 181 EIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVDGGM 240
           EIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVDGGM
Sbjct: 181 EIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVDGGM 240

Query: 241 VRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGI 300
           VRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGI
Sbjct: 241 VRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGI 300

Query: 301 AEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASDGAM 360
           AEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASDGAM
Sbjct: 301 AEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASDGAM 360

Query: 361 VARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEA 420
           VARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEA
Sbjct: 361 VARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEA 420

Query: 421 VRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGFSFSDSI 480
           VRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVG SFSDSI
Sbjct: 421 VRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSSFSDSI 480

Query: 481 LEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWG 540
           LEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWG
Sbjct: 481 LEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWG 540

Query: 541 LIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 592
           LIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 LIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 591

[Close]

BLAST of Chy5G094180 vs. ExPASy TrEMBL
Match: A0A5A7UTK2 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold216G001510 PE=3 SV=1)

HSP 1 Score: 1108.6 bits (2866), Expect = 0.0e+00
Identity = 582/591 (98.48%), Postives = 587/591 (99.32%), Query Frame = 0


Query: 1   MTQSLQIFTSSAISLPKHSISKPSSSSAAFRFPFSFSKSSIRASSSSDLNPLSSSSTSQV 60
           MTQSLQ+FTSSAISLPKHSISKP SS+AAFRFPFSFSKSSIRA SSSDLNPLSSSSTSQV
Sbjct: 1   MTQSLQLFTSSAISLPKHSISKPFSSTAAFRFPFSFSKSSIRA-SSSDLNPLSSSSTSQV 60

Query: 61  LVSENGSSSGGGVVFSSAATREFVSVASDSTSIEVDAVTEAELKENGFRSTRRTKLVCTI 120
           LVSENGSSSGGGVV SS+ATREFVSVASDSTSI+VDAVTEAELKENGFRSTRRTKLVCTI
Sbjct: 61  LVSENGSSSGGGVV-SSSATREFVSVASDSTSIDVDAVTEAELKENGFRSTRRTKLVCTI 120

Query: 121 GPATCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMDTEGS 180
           GPA+CGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMDTEGS
Sbjct: 121 GPASCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMDTEGS 180

Query: 181 EIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVDGGM 240
           EIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVDGGM
Sbjct: 181 EIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVDGGM 240

Query: 241 VRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGI 300
           VRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGI
Sbjct: 241 VRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGI 300

Query: 301 AEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASDGAM 360
           AEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASDGAM
Sbjct: 301 AEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASDGAM 360

Query: 361 VARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEA 420
           VARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEA
Sbjct: 361 VARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEA 420

Query: 421 VRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGFSFSDSI 480
           VRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVG SFSDSI
Sbjct: 421 VRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSSFSDSI 480

Query: 481 LEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWG 540
           LEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWG
Sbjct: 481 LEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWG 540

Query: 541 LIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 592
           LIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 LIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 589

[Close]

BLAST of Chy5G094180 vs. ExPASy TrEMBL
Match: A0A1S3BLQ9 (Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103491219 PE=3 SV=1)

HSP 1 Score: 1108.6 bits (2866), Expect = 0.0e+00
Identity = 582/591 (98.48%), Postives = 587/591 (99.32%), Query Frame = 0


Query: 1   MTQSLQIFTSSAISLPKHSISKPSSSSAAFRFPFSFSKSSIRASSSSDLNPLSSSSTSQV 60
           MTQSLQ+FTSSAISLPKHSISKP SS+AAFRFPFSFSKSSIRA SSSDLNPLSSSSTSQV
Sbjct: 1   MTQSLQLFTSSAISLPKHSISKPFSSTAAFRFPFSFSKSSIRA-SSSDLNPLSSSSTSQV 60

Query: 61  LVSENGSSSGGGVVFSSAATREFVSVASDSTSIEVDAVTEAELKENGFRSTRRTKLVCTI 120
           LVSENGSSSGGGVV SS+ATREFVSVASDSTSI+VDAVTEAELKENGFRSTRRTKLVCTI
Sbjct: 61  LVSENGSSSGGGVV-SSSATREFVSVASDSTSIDVDAVTEAELKENGFRSTRRTKLVCTI 120

Query: 121 GPATCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMDTEGS 180
           GPA+CGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMDTEGS
Sbjct: 121 GPASCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMDTEGS 180

Query: 181 EIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVDGGM 240
           EIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVDGGM
Sbjct: 181 EIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVDGGM 240

Query: 241 VRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGI 300
           VRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGI
Sbjct: 241 VRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGI 300

Query: 301 AEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASDGAM 360
           AEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASDGAM
Sbjct: 301 AEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASDGAM 360

Query: 361 VARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEA 420
           VARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEA
Sbjct: 361 VARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEA 420

Query: 421 VRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGFSFSDSI 480
           VRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVG SFSDSI
Sbjct: 421 VRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSSFSDSI 480

Query: 481 LEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWG 540
           LEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWG
Sbjct: 481 LEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWG 540

Query: 541 LIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 592
           LIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 LIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 589

[Close]

BLAST of Chy5G094180 vs. ExPASy TrEMBL
Match: A0A6J1KDQ9 (Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111494735 PE=3 SV=1)

HSP 1 Score: 1056.6 bits (2731), Expect = 3.8e-305
Identity = 551/597 (92.29%), Postives = 571/597 (95.64%), Query Frame = 0


Query: 1   MTQSLQIFTSS-----AISLPKHSISK-PSSSSAAFRFPFSFSKSSIRASSSSDLNPLSS 60
           M QSLQ+FTSS     +ISLPKHS+SK PSSS+AAFR P SF+K SIRASSS DLNPLSS
Sbjct: 1   MAQSLQLFTSSTTRSPSISLPKHSLSKTPSSSTAAFRLPVSFNKFSIRASSSPDLNPLSS 60

Query: 61  SSTSQVLVSENGSSSGGGVVFSSAATREFVSVASDSTSIEVDAVTEAELKENGFRSTRRT 120
           +STSQVLVSENGSSSGGG V +SA TREFV   SD +SI+VD VTEAELKENGFRSTRRT
Sbjct: 61  ASTSQVLVSENGSSSGGGFVSTSATTREFVPAVSDGSSIDVDTVTEAELKENGFRSTRRT 120

Query: 121 KLVCTIGPATCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIM 180
           KLVCTIGP TCGFEQLEALAVGGMNVARINMCHGTR+WHR VIERVRRLN+EKGYAVAIM
Sbjct: 121 KLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGYAVAIM 180

Query: 181 MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL 240
           MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL
Sbjct: 181 MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL 240

Query: 241 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 300
           LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL
Sbjct: 241 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 300

Query: 301 DIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIL 360
           DIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEII+
Sbjct: 301 DIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIV 360

Query: 361 ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEV 420
           ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCR+LNKPVIVASQLLESMIEYPTPTRAEV
Sbjct: 361 ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTPTRAEV 420

Query: 421 ADVSEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGF 480
           ADVSEAVRQR+DALMLSGESAMGQ+P+KAL VLRSVSLRIEKWWRDEKRHE MELPEVG 
Sbjct: 421 ADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMELPEVGS 480

Query: 481 SFSDSILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRR 540
           S+SDSILEEICNS AKMANNLEVDAIFVYT +GHMASLLSRCRPDCPIFAFTSTTSVRRR
Sbjct: 481 SYSDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTTSVRRR 540

Query: 541 LNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 592
           LNLQWGLIPFRLSFSDDMENNLNKTF+LLKARNLIKSGDLV+AVSDMLQSIQVMNVP
Sbjct: 541 LNLQWGLIPFRLSFSDDMENNLNKTFMLLKARNLIKSGDLVLAVSDMLQSIQVMNVP 597

[Close]

BLAST of Chy5G094180 vs. ExPASy TrEMBL
Match: A0A6J1L0W9 (Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111499365 PE=3 SV=1)

HSP 1 Score: 1056.2 bits (2730), Expect = 5.0e-305
Identity = 552/595 (92.77%), Postives = 568/595 (95.46%), Query Frame = 0


Query: 1   MTQSLQIFTSS----AISLPKHSISKPSSSSAAFRFPFSFSKSSIRASSSSDLNPLSSSS 60
           MTQSLQIFTS+     ISL KHS+SKPS S+AAFRFP SF+KSSIRASSS D NPLSS+ 
Sbjct: 1   MTQSLQIFTSATRSPGISLSKHSLSKPSPSTAAFRFPLSFTKSSIRASSSPDSNPLSSAF 60

Query: 61  TSQVLVSENGSSSGGGVVFSSAATREFVSVASDSTSIEVDAVTEAELKENGFRSTRRTKL 120
           TSQVLVSENGS+ GGG + SS+ TREFV V SDST+IEVD VTEAELKENGFRSTRRTKL
Sbjct: 61  TSQVLVSENGSTGGGGAL-SSSVTREFVPVMSDSTAIEVDTVTEAELKENGFRSTRRTKL 120

Query: 121 VCTIGPATCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMD 180
           VCTIGP TCGFEQLEALAVGGMNVARINMCHGTRDWHR VIERVRRLN+EKGYAVAIMMD
Sbjct: 121 VCTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAVAIMMD 180

Query: 181 TEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLV 240
           TEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLV
Sbjct: 181 TEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLV 240

Query: 241 DGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDI 300
           DGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDI
Sbjct: 241 DGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDI 300

Query: 301 DFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILAS 360
           DFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISI+AKIESLDSL NLEEIILAS
Sbjct: 301 DFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISILAKIESLDSLNNLEEIILAS 360

Query: 361 DGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVAD 420
           DGAMVARGDLGAQIPLEQVP VQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVAD
Sbjct: 361 DGAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVAD 420

Query: 421 VSEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGFSF 480
           +SEAVRQRSDALMLSGESAMGQYPDKALAVLR+VSLRIEKWWR+EKRHEPMELPEVG S 
Sbjct: 421 ISEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGSSS 480

Query: 481 SDSILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLN 540
           SDSILEEICNSAAKMANNLEVDAIFVYT +GHMASLLSRCRPDCPIFAFT TTSVRRRLN
Sbjct: 481 SDSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRRLN 540

Query: 541 LQWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 592
           LQWGLIPFR+SFSDDMENNLNK FLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 LQWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 594

[Close]

BLAST of Chy5G094180 vs. NCBI nr
Match: XP_004150564.1 (plastidial pyruvate kinase 1, chloroplastic [Cucumis sativus] >KGN61654.1 hypothetical protein Csa_006584 [Cucumis sativus])

HSP 1 Score: 1121 bits (2899), Expect = 0.0
Identity = 588/591 (99.49%), Postives = 589/591 (99.66%), Query Frame = 0


Query: 1   MTQSLQIFTSSAISLPKHSISKPSSSSAAFRFPFSFSKSSIRASSSSDLNPLSSSSTSQV 60
           MTQSLQ+FTSSAISLPKHSISKPSSSSAAFRFPFSFSKSSIRASSSSDLNPLSSSSTSQV
Sbjct: 1   MTQSLQLFTSSAISLPKHSISKPSSSSAAFRFPFSFSKSSIRASSSSDLNPLSSSSTSQV 60

Query: 61  LVSENGSSSGGGVVFSSAATREFVSVASDSTSIEVDAVTEAELKENGFRSTRRTKLVCTI 120
           LVSENGSSSGGGVVFSSAATREFVSVASDSTSIEVDAVTEAELKENGFRSTRRTKLVCTI
Sbjct: 61  LVSENGSSSGGGVVFSSAATREFVSVASDSTSIEVDAVTEAELKENGFRSTRRTKLVCTI 120

Query: 121 GPATCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMDTEGS 180
           GPATCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLND KGYAVAIMMDTEGS
Sbjct: 121 GPATCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDGKGYAVAIMMDTEGS 180

Query: 181 EIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVDGGM 240
           EIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVDGGM
Sbjct: 181 EIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVDGGM 240

Query: 241 VRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGI 300
           VRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGI
Sbjct: 241 VRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGI 300

Query: 301 AEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASDGAM 360
           AEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASDGAM
Sbjct: 301 AEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASDGAM 360

Query: 361 VARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEA 420
           VARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEA
Sbjct: 361 VARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEA 420

Query: 421 VRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGFSFSDSI 480
           VRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVG SFSDSI
Sbjct: 421 VRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSSFSDSI 480

Query: 481 LEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWG 540
           LEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWG
Sbjct: 481 LEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWG 540

Query: 541 LIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 591
           LIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 LIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 591

[Close]

BLAST of Chy5G094180 vs. NCBI nr
Match: XP_008449299.1 (PREDICTED: plastidial pyruvate kinase 1, chloroplastic [Cucumis melo] >KAA0057466.1 plastidial pyruvate kinase 1 [Cucumis melo var. makuwa] >TYK30163.1 plastidial pyruvate kinase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1104 bits (2855), Expect = 0.0
Identity = 582/591 (98.48%), Postives = 587/591 (99.32%), Query Frame = 0


Query: 1   MTQSLQIFTSSAISLPKHSISKPSSSSAAFRFPFSFSKSSIRASSSSDLNPLSSSSTSQV 60
           MTQSLQ+FTSSAISLPKHSISKP SS+AAFRFPFSFSKSSIRA SSSDLNPLSSSSTSQV
Sbjct: 1   MTQSLQLFTSSAISLPKHSISKPFSSTAAFRFPFSFSKSSIRA-SSSDLNPLSSSSTSQV 60

Query: 61  LVSENGSSSGGGVVFSSAATREFVSVASDSTSIEVDAVTEAELKENGFRSTRRTKLVCTI 120
           LVSENGSSSGGGVV SS+ATREFVSVASDSTSI+VDAVTEAELKENGFRSTRRTKLVCTI
Sbjct: 61  LVSENGSSSGGGVV-SSSATREFVSVASDSTSIDVDAVTEAELKENGFRSTRRTKLVCTI 120

Query: 121 GPATCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMDTEGS 180
           GPA+CGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMDTEGS
Sbjct: 121 GPASCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMDTEGS 180

Query: 181 EIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVDGGM 240
           EIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVDGGM
Sbjct: 181 EIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVDGGM 240

Query: 241 VRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGI 300
           VRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGI
Sbjct: 241 VRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGI 300

Query: 301 AEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASDGAM 360
           AEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASDGAM
Sbjct: 301 AEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASDGAM 360

Query: 361 VARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEA 420
           VARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEA
Sbjct: 361 VARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEA 420

Query: 421 VRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGFSFSDSI 480
           VRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVG SFSDSI
Sbjct: 421 VRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSSFSDSI 480

Query: 481 LEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWG 540
           LEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWG
Sbjct: 481 LEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWG 540

Query: 541 LIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 591
           LIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 LIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 589

[Close]

BLAST of Chy5G094180 vs. NCBI nr
Match: XP_038874381.1 (pyruvate kinase isozyme A, chloroplastic [Benincasa hispida])

HSP 1 Score: 1086 bits (2808), Expect = 0.0
Identity = 572/595 (96.13%), Postives = 584/595 (98.15%), Query Frame = 0


Query: 1   MTQSLQIFTSSA----ISLPKHSISKPSSSSAAFRFPFSFSKSSIRASSSSDLNPLSSSS 60
           MTQSLQ+FTSS     ISLP+ S+SKPSSSSAAFRFPFSFSKSSIRASSS DLNPLSSSS
Sbjct: 1   MTQSLQLFTSSTRSPGISLPRRSLSKPSSSSAAFRFPFSFSKSSIRASSSPDLNPLSSSS 60

Query: 61  TSQVLVSENGSSSGGGVVFSSAATREFVSVASDSTSIEVDAVTEAELKENGFRSTRRTKL 120
           TSQVLVSENGSSSG GVV SS+AT+EFVS+ASDSTSI+VDAVTEAELKENGFRSTRRTKL
Sbjct: 61  TSQVLVSENGSSSGTGVV-SSSATKEFVSLASDSTSIDVDAVTEAELKENGFRSTRRTKL 120

Query: 121 VCTIGPATCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMD 180
           VCTIGPA+CGFEQLEALAVGGMNVARINMCHGTR+WHRTVIE VRRLNDEKGYAVAIMMD
Sbjct: 121 VCTIGPASCGFEQLEALAVGGMNVARINMCHGTREWHRTVIEYVRRLNDEKGYAVAIMMD 180

Query: 181 TEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLV 240
           TEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLV
Sbjct: 181 TEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLV 240

Query: 241 DGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDI 300
           DGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDI
Sbjct: 241 DGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDI 300

Query: 301 DFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILAS 360
           DFGIAEGVDF+AISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEII+AS
Sbjct: 301 DFGIAEGVDFIAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIVAS 360

Query: 361 DGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVAD 420
           DGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVAD
Sbjct: 361 DGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVAD 420

Query: 421 VSEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGFSF 480
           VSEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVG SF
Sbjct: 421 VSEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSSF 480

Query: 481 SDSILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLN 540
           SD+ILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLN
Sbjct: 481 SDTILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLN 540

Query: 541 LQWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 591
           LQWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 LQWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 594

[Close]

BLAST of Chy5G094180 vs. NCBI nr
Match: XP_023006710.1 (pyruvate kinase isozyme A, chloroplastic-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1051 bits (2719), Expect = 0.0
Identity = 552/595 (92.77%), Postives = 568/595 (95.46%), Query Frame = 0


Query: 1   MTQSLQIFTSSA----ISLPKHSISKPSSSSAAFRFPFSFSKSSIRASSSSDLNPLSSSS 60
           MTQSLQIFTS+     ISL KHS+SKPS S+AAFRFP SF+KSSIRASSS D NPLSS+ 
Sbjct: 1   MTQSLQIFTSATRSPGISLSKHSLSKPSPSTAAFRFPLSFTKSSIRASSSPDSNPLSSAF 60

Query: 61  TSQVLVSENGSSSGGGVVFSSAATREFVSVASDSTSIEVDAVTEAELKENGFRSTRRTKL 120
           TSQVLVSENGS+ GGG + SS+ TREFV V SDST+IEVD VTEAELKENGFRSTRRTKL
Sbjct: 61  TSQVLVSENGSTGGGGAL-SSSVTREFVPVMSDSTAIEVDTVTEAELKENGFRSTRRTKL 120

Query: 121 VCTIGPATCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMD 180
           VCTIGP TCGFEQLEALAVGGMNVARINMCHGTRDWHR VIERVRRLN+EKGYAVAIMMD
Sbjct: 121 VCTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAVAIMMD 180

Query: 181 TEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLV 240
           TEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLV
Sbjct: 181 TEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLV 240

Query: 241 DGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDI 300
           DGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDI
Sbjct: 241 DGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDI 300

Query: 301 DFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILAS 360
           DFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISI+AKIESLDSL NLEEIILAS
Sbjct: 301 DFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISILAKIESLDSLNNLEEIILAS 360

Query: 361 DGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVAD 420
           DGAMVARGDLGAQIPLEQVP VQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVAD
Sbjct: 361 DGAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVAD 420

Query: 421 VSEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGFSF 480
           +SEAVRQRSDALMLSGESAMGQYPDKALAVLR+VSLRIEKWWR+EKRHEPMELPEVG S 
Sbjct: 421 ISEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGSSS 480

Query: 481 SDSILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLN 540
           SDSILEEICNSAAKMANNLEVDAIFVYT +GHMASLLSRCRPDCPIFAFT TTSVRRRLN
Sbjct: 481 SDSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRRLN 540

Query: 541 LQWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 591
           LQWGLIPFR+SFSDDMENNLNK FLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 LQWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 594

[Close]

BLAST of Chy5G094180 vs. NCBI nr
Match: XP_023000477.1 (pyruvate kinase isozyme A, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 1051 bits (2719), Expect = 0.0
Identity = 551/597 (92.29%), Postives = 571/597 (95.64%), Query Frame = 0


Query: 1   MTQSLQIFTSS-----AISLPKHSISK-PSSSSAAFRFPFSFSKSSIRASSSSDLNPLSS 60
           M QSLQ+FTSS     +ISLPKHS+SK PSSS+AAFR P SF+K SIRASSS DLNPLSS
Sbjct: 1   MAQSLQLFTSSTTRSPSISLPKHSLSKTPSSSTAAFRLPVSFNKFSIRASSSPDLNPLSS 60

Query: 61  SSTSQVLVSENGSSSGGGVVFSSAATREFVSVASDSTSIEVDAVTEAELKENGFRSTRRT 120
           +STSQVLVSENGSSSGGG V +SA TREFV   SD +SI+VD VTEAELKENGFRSTRRT
Sbjct: 61  ASTSQVLVSENGSSSGGGFVSTSATTREFVPAVSDGSSIDVDTVTEAELKENGFRSTRRT 120

Query: 121 KLVCTIGPATCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIM 180
           KLVCTIGP TCGFEQLEALAVGGMNVARINMCHGTR+WHR VIERVRRLN+EKGYAVAIM
Sbjct: 121 KLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGYAVAIM 180

Query: 181 MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL 240
           MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL
Sbjct: 181 MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL 240

Query: 241 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 300
           LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL
Sbjct: 241 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 300

Query: 301 DIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIL 360
           DIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEII+
Sbjct: 301 DIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIV 360

Query: 361 ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEV 420
           ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCR+LNKPVIVASQLLESMIEYPTPTRAEV
Sbjct: 361 ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTPTRAEV 420

Query: 421 ADVSEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGF 480
           ADVSEAVRQR+DALMLSGESAMGQ+P+KAL VLRSVSLRIEKWWRDEKRHE MELPEVG 
Sbjct: 421 ADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMELPEVGS 480

Query: 481 SFSDSILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRR 540
           S+SDSILEEICNS AKMANNLEVDAIFVYT +GHMASLLSRCRPDCPIFAFTSTTSVRRR
Sbjct: 481 SYSDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTTSVRRR 540

Query: 541 LNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 591
           LNLQWGLIPFRLSFSDDMENNLNKTF+LLKARNLIKSGDLV+AVSDMLQSIQVMNVP
Sbjct: 541 LNLQWGLIPFRLSFSDDMENNLNKTFMLLKARNLIKSGDLVLAVSDMLQSIQVMNVP 597

[Close]

BLAST of Chy5G094180 vs. TAIR 10
Match: AT3G22960.1 (Pyruvate kinase family protein )

HSP 1 Score: 922.5 bits (2383), Expect = 1.7e-268
Identity = 482/600 (80.33%), Postives = 533/600 (88.83%), Query Frame = 0


Query: 1   MTQSLQIFTSS----AISLPKHSISKPSSSSAAFRFPFSFSK-SSIRASSSS----DLNP 60
           M+QS+Q  T S     + LP    ++P SS +  RFP +  K +SIRASSSS    DL+ 
Sbjct: 1   MSQSIQFSTPSHTPHLLHLPHSQFNRPLSSISFRRFPLTTIKYTSIRASSSSSPSPDLDS 60

Query: 61  LSSSSTSQVLVSENGSSSGGGVVFSSAATREFVSVASDSTSIEVDAVTEAELKENGFRST 120
            SSSS+SQVL+S NG+    G V S   +    +V +D++ IEVD VTEAELKENGFRST
Sbjct: 61  SSSSSSSQVLLSPNGT----GAVKSDERSVVATAVTTDTSGIEVDTVTEAELKENGFRST 120

Query: 121 RRTKLVCTIGPATCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAV 180
           RRTKL+CTIGPATCGFEQLEALAVGGMNVAR+NMCHGTRDWHR VI  VRRLN+EKG+AV
Sbjct: 121 RRTKLICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDWHRGVIRSVRRLNEEKGFAV 180

Query: 181 AIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVG 240
           AIMMDTEGSEIHMGDLGG +SAKAEDGE+WTF+VRAFDS+ PERTI+V+Y+GFAEDVRVG
Sbjct: 181 AIMMDTEGSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSSRPERTISVSYDGFAEDVRVG 240

Query: 241 DDLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSK 300
           D+LLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDG LVRERNAMLPTISSK
Sbjct: 241 DELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGSLVRERNAMLPTISSK 300

Query: 301 DWLDIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEE 360
           DWLDIDFGIAEGVDF+A+SFVKSAEVI HLKSY+AARSRG +I +IAKIES+DSL NLEE
Sbjct: 301 DWLDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRGGEIGVIAKIESIDSLTNLEE 360

Query: 361 IILASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTR 420
           IILASDGAMVARGDLGAQIPLEQVP+ QQ++VQ+CR LNKPVIVASQLLESMIEYPTPTR
Sbjct: 361 IILASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNKPVIVASQLLESMIEYPTPTR 420

Query: 421 AEVADVSEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPE 480
           AEVADVSEAVRQRSDALMLSGESAMGQ+PDKAL VLR+VSLRIE+WWR+EKRHE + L  
Sbjct: 421 AEVADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVSLRIERWWREEKRHESVPLQA 480

Query: 481 VGFSFSDSILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSV 540
           +G SFSD I EEICNSAAKMANNL VDA+FVYTTSGHMASL+SRCRPDCPIFAFT+TTSV
Sbjct: 481 IGSSFSDKISEEICNSAAKMANNLGVDAVFVYTTSGHMASLVSRCRPDCPIFAFTTTTSV 540

Query: 541 RRRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 592
           RRRLNLQWGLIPFRLSFSDDME+NLNKTF LLK+R +IKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 RRRLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSRGMIKSGDLVIAVSDMLQSIQVMNVP 596

[Close]

BLAST of Chy5G094180 vs. TAIR 10
Match: AT5G52920.1 (plastidic pyruvate kinase beta subunit 1 )

HSP 1 Score: 364.0 bits (933), Expect = 2.3e-100
Identity = 207/475 (43.58%), Postives = 292/475 (61.47%), Query Frame = 0


Query: 112 RRTKLVCTIGPATCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDE-KGYA 171
           R+TK+VCT+GP+T   E +  LA  GMNVAR+NM HG    H+ VI+ V+  N + K   
Sbjct: 110 RKTKIVCTVGPSTNTREMIWKLAEAGMNVARMNMSHGDHASHKKVIDLVKEYNAQTKDNT 169

Query: 172 VAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRV 231
           +AIM+DT+G E+  GDL        + G+ +TF++    ST     ++VNY+ F  DV  
Sbjct: 170 IAIMLDTKGPEVRSGDL--PQPIMLDPGQEFTFTIERGVST--PSCVSVNYDDFVNDVEA 229

Query: 232 GDDLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISS 291
           GD LLVDGGM+ F V  K    VKC   D G L  R +L        VR ++A LP+I+ 
Sbjct: 230 GDMLLVDGGMMSFMVKSKTKDSVKCEVVDGGELKSRRHLN-------VRGKSATLPSITE 289

Query: 292 KDWLDIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLE 351
           KDW DI FG+   VDF A+SFVK A+V+  LK Y+  ++ G+DI +I KIES DS+ NL 
Sbjct: 290 KDWEDIKFGVENKVDFYAVSFVKDAQVVHELKKYL--QNSGADIHVIVKIESADSIPNLH 349

Query: 352 EIILASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPT 411
            II ASDGAMVARGDLGA++P+E+VP +Q++++ LCR + K VIVA+ +LESMI +PTPT
Sbjct: 350 SIITASDGAMVARGDLGAELPIEEVPILQEEIINLCRSMGKAVIVATNMLESMIVHPTPT 409

Query: 412 RAEVADVSEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELP 471
           RAEV+D++ AVR+ +DA+MLSGE+A G++P KA  V+ +V+LR E      +       P
Sbjct: 410 RAEVSDIAIAVREGADAVMLSGETAHGKFPLKAAGVMHTVALRTEATITSGEMP-----P 469

Query: 472 EVGFSFSDSILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTS 531
            +G +F + + E     A  M+N L    + V+T +G MA LLS  RP   I+AFT+   
Sbjct: 470 NLGQAFKNHMSEMFAYHATMMSNTLGTSTV-VFTRTGFMAILLSHYRPSGTIYAFTNEKK 529

Query: 532 VRRRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSI 586
           +++RL L  G+ P  + F+DD E         L  + ++K G+ +  V    Q I
Sbjct: 530 IQQRLALYQGVCPIYMEFTDDAEETFANALATLLKQGMVKKGEEIAIVQSGTQPI 565

[Close]

BLAST of Chy5G094180 vs. TAIR 10
Match: AT1G32440.1 (plastidial pyruvate kinase 3 )

HSP 1 Score: 360.5 bits (924), Expect = 2.5e-99
Identity = 214/490 (43.67%), Postives = 304/490 (62.04%), Query Frame = 0


Query: 101 AELKENGFRSTRRTKLVCTIGPATCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERV 160
           A+ + +   S R+TK+VCTIGP++   E +  LA  GMNVAR+NM HG    H+  I+ V
Sbjct: 88  ADSRTSSNDSRRKTKIVCTIGPSSSSREMIWKLAEAGMNVARLNMSHGDHASHQITIDLV 147

Query: 161 RRLND-EKGYAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINV 220
           +  N      A+AIM+DT+G E+  GD+        E+G+ + F+++   S   + T++V
Sbjct: 148 KEYNSLFVDKAIAIMLDTKGPEVRSGDV--PQPIFLEEGQEFNFTIKRGVSL--KDTVSV 207

Query: 221 NYEGFAEDVRVGDDLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVR 280
           NY+ F  DV VGD LLVDGGM+   V  K    VKC+  D G L  R +L        VR
Sbjct: 208 NYDDFVNDVEVGDILLVDGGMMSLAVKSKTSDLVKCVVIDGGELQSRRHLN-------VR 267

Query: 281 ERNAMLPTISSKDWLDIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAK 340
            ++A LP+I+ KDW DI FG+   VDF A+SFVK A+V+  LK+Y+  ++  +DIS+I K
Sbjct: 268 GKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDAKVVHELKNYL--KTCSADISVIVK 327

Query: 341 IESLDSLKNLEEIILASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQL 400
           IES DS+KNL  II A DGAMVARGDLGA++P+E+VP +Q+++++ CR ++KPVIVA+ +
Sbjct: 328 IESADSIKNLPSIISACDGAMVARGDLGAELPIEEVPLLQEEIIRRCRSIHKPVIVATNM 387

Query: 401 LESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWW- 460
           LESMI +PTPTRAEV+D++ AVR+ +DA+MLSGE+A G++P KA+ V+ +V+LR E    
Sbjct: 388 LESMINHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFPLKAVNVMHTVALRTEASLP 447

Query: 461 -RDEKRHEPMELPEVG--FSFSDSILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSR 520
            R            +G  F+F  SI          MAN L    + V+T +G MA LLS 
Sbjct: 448 VRTSASRTTAYKGHMGQMFAFHASI----------MANTLS-SPLIVFTRTGSMAVLLSH 507

Query: 521 CRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLV 580
            RP   IFAFT+   + +RL L  G++P  + FSDD E+   ++  LL+  N++K G  V
Sbjct: 508 YRPSATIFAFTNQRRIMQRLALYQGVMPIYMEFSDDAEDTYARSLKLLQDENMLKEGQHV 553

Query: 581 IAVSDMLQSI 586
             V    Q I
Sbjct: 568 TLVQSGSQPI 553

[Close]

BLAST of Chy5G094180 vs. TAIR 10
Match: AT5G63680.1 (Pyruvate kinase family protein )

HSP 1 Score: 240.4 bits (612), Expect = 3.8e-63
Identity = 160/516 (31.01%), Postives = 270/516 (52.33%), Query Frame = 0


Query: 91  TSIEVDAVTEAELKENGFRSTRRTKLVCTIGPATCGFEQLEALAVGGMNVARINMCHGTR 150
           ++I+++ + + EL  +G   T +TK+VCT+GPA+     +E L   GMNVAR N  HG+ 
Sbjct: 2   SNIDIEGILK-ELPNDG--RTPKTKIVCTLGPASRSVTMIEKLLKAGMNVARFNFSHGSH 61

Query: 151 DWHRTVIERVRRLNDEKGYAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDS 210
           ++H+  ++ +R      G   A+M+DT+G EI  G L   +  + ++G+  T +   +D 
Sbjct: 62  EYHQETLDNLRTAMQNTGILAAVMLDTKGPEIRTGFLKDGNPIQLKEGQEITITT-DYDI 121

Query: 211 TLPERTINVNYEGFAEDVRVGDDLLVDGGMVRFEVI--EKIGPDVKCLCTDPGLLLPRAN 270
              E+TI+++Y+    DV+ G+ +L   G +   V+  +     V C C +  +L  R N
Sbjct: 122 KGDEKTISMSYKKLPVDVKPGNTILCADGSISLAVVSCDPNAGTVICRCENTAMLGERKN 181

Query: 271 LTFWRDGHLVRERNAMLPTISSKDWLDI-DFGIAEGVDFLAISFVKSAEVIKHLKSYIAA 330
           +     G +V      LPT++ KD  DI  +G+   +D +A+SFV+    + +++  + +
Sbjct: 182 VNL--PGVVVD-----LPTLTDKDVEDILKWGVPNNIDMIALSFVRKGSDLVNVRKVLGS 241

Query: 331 RSRGSDISIIAKIESLDSLKNLEEIILASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCR 390
            S+   I +++K+E+ + + N +EI+  +D  MVARGDLG +IP+E++   Q+ ++  C 
Sbjct: 242 HSK--SIMLMSKVENQEGVLNFDEILRETDAFMVARGDLGMEIPIEKIFLAQKMMIYKCN 301

Query: 391 QLNKPVIVASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQYPDKALAVL 450
              KPV+ A+Q+LESMI+ P PTRAE  DV+ AV   +D +MLSGESA G YP+ A+  +
Sbjct: 302 LAGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGAYPEIAVKTM 361

Query: 451 RSVSLRIEK------WWRDEKRHEPMELPEVGFSFSDSILEEICNSAAKMANNLEVDAIF 510
             + +  E        +++  R  P+ +         S LE + +SA + AN  +   I 
Sbjct: 362 AKICIEAESSLDYNTIFKEMIRATPLPM---------STLESLASSAVRTANKAKAKLII 421

Query: 511 VYTTSGHMASLLSRCRPDCPIFA-----FTSTT--------SVRRRLNLQWGLIPF---- 570
           V T  G  A L+++ RP  PI +     FTS T        S  R   +  GLIP     
Sbjct: 422 VLTRGGTTAKLVAKYRPAVPILSVVVPVFTSDTFNWSCSDESPARHSLIYRGLIPVLGEG 481

Query: 571 --RLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAV 579
             + + S+  E  +         + L   GD V+A+
Sbjct: 482 SAKATDSESTEEIIESALKSATEKGLCNHGDAVVAL 495

[Close]

BLAST of Chy5G094180 vs. TAIR 10
Match: AT5G08570.1 (Pyruvate kinase family protein )

HSP 1 Score: 233.0 bits (593), Expect = 6.1e-61
Identity = 157/516 (30.43%), Postives = 265/516 (51.36%), Query Frame = 0


Query: 91  TSIEVDAVTEAELKENGFRSTRRTKLVCTIGPATCGFEQLEALAVGGMNVARINMCHGTR 150
           ++I+++ + + EL  +G     +TK+VCT+GPA+     +E L   GMNVAR N  HG+ 
Sbjct: 2   SNIDIEGILK-ELPNDG--RIPKTKIVCTLGPASRTVSMIEKLLKAGMNVARFNFSHGSH 61

Query: 151 DWHRTVIERVRRLNDEKGYAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDS 210
           ++H+  ++ +R      G   A+M+DT+G EI  G L   +  + ++G+  T +   +D 
Sbjct: 62  EYHQETLDNLRSAMHNTGILAAVMLDTKGPEIRTGFLKDGNPIQLKEGQEITITT-DYDI 121

Query: 211 TLPERTINVNYEGFAEDVRVGDDLLVDGGMVRFEVI--EKIGPDVKCLCTDPGLLLPRAN 270
              E TI+++Y+    DV+ G+ +L   G +   V+  +     V+C C +  +L  R N
Sbjct: 122 QGDESTISMSYKKLPLDVKPGNTILCADGSISLAVLSCDPESGTVRCRCENSAMLGERKN 181

Query: 271 LTFWRDGHLVRERNAMLPTISSKDWLDI-DFGIAEGVDFLAISFVKSAEVIKHLKSYIAA 330
           +     G +V      LPT++ KD  DI  +G+   +D +A+SFV+    + +++  +  
Sbjct: 182 VNL--PGVVVD-----LPTLTDKDIEDILGWGVPNSIDMIALSFVRKGSDLVNVRKVLG- 241

Query: 331 RSRGSDISIIAKIESLDSLKNLEEIILASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCR 390
            S    I +++K+E+ + + N +EI+  +D  MVARGDLG +IP+E++   Q+ ++  C 
Sbjct: 242 -SHAKSIMLMSKVENQEGVINFDEILRETDAFMVARGDLGMEIPIEKIFLAQKLMIYKCN 301

Query: 391 QLNKPVIVASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQYPDKALAVL 450
              KPV+ A+Q+LESMI+ P PTRAE  DV+ AV   +D +MLSGESA G YP+ A+ V+
Sbjct: 302 LAGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGAYPEIAVKVM 361

Query: 451 RSVSLRIEK------WWRDEKRHEPMELPEVGFSFSDSILEEICNSAAKMANNLEVDAIF 510
             + +  E        +++  R  P+ +         S LE + +SA + AN      I 
Sbjct: 362 AKICIEAESSLDYNTIFKEMIRATPLPM---------SPLESLASSAVRTANKARAKLII 421

Query: 511 VYTTSGHMASLLSRCRPDCPIFAFT-------------STTSVRRRLNLQWGLIPF---- 570
           V T  G  A+L+++ RP  PI +               S  S  R   +  GLIP     
Sbjct: 422 VLTRGGSTANLVAKYRPAVPILSVVVPVMTTDSFDWSCSDESPARHSLIYRGLIPMLAEG 481

Query: 571 --RLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAV 579
             + + S+  E  +         R L   GD ++A+
Sbjct: 482 SAKATDSEATEVIIEAALKSATQRGLCNRGDAIVAL 495

The following BLAST results are available for this feature:

BLAST of Chy5G094180 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LIK0	2.3e-267	80.33	Plastidial pyruvate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=P...	[more]
Q43117	4.4e-266	81.85	Pyruvate kinase isozyme A, chloroplastic OS=Ricinus communis OX=3988 PE=1 SV=1	[more]
Q40545	7.8e-263	79.77	Pyruvate kinase isozyme A, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
Q40546	1.7e-100	44.03	Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
Q9FLW9	3.2e-99	43.58	Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1	[more]

BLAST of Chy5G094180 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LKS3	0.0e+00	99.49	Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_2G215480 PE=3 SV=1	[more]
A0A5A7UTK2	0.0e+00	98.48	Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold216G0015...	[more]
A0A1S3BLQ9	0.0e+00	98.48	Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103491219 PE=3 SV=1	[more]
A0A6J1KDQ9	3.8e-305	92.29	Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111494735 PE=3 SV=1	[more]
A0A6J1L0W9	5.0e-305	92.77	Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111499365 PE=3 SV=1	[more]

BLAST of Chy5G094180 vs. NCBI nr


Match Name	Identity	Description
XP_004150564.1	99.49	plastidial pyruvate kinase 1, chloroplastic [Cucumis sativus] >KGN61654.1 hypoth...	[more]
XP_008449299.1	98.48	PREDICTED: plastidial pyruvate kinase 1, chloroplastic [Cucumis melo] >KAA005746...	[more]
XP_038874381.1	96.13	pyruvate kinase isozyme A, chloroplastic [Benincasa hispida]	[more]
XP_023006710.1	92.77	pyruvate kinase isozyme A, chloroplastic-like isoform X2 [Cucurbita maxima]	[more]
XP_023000477.1	92.29	pyruvate kinase isozyme A, chloroplastic-like [Cucurbita maxima]	[more]

BLAST of Chy5G094180 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G22960.1	1.7e-268	80.33	Pyruvate kinase family protein	[more]
AT5G52920.1	2.3e-100	43.58	plastidic pyruvate kinase beta subunit 1	[more]
AT1G32440.1	2.5e-99	43.67	plastidial pyruvate kinase 3	[more]
AT5G63680.1	3.8e-63	31.01	Pyruvate kinase family protein	[more]
AT5G08570.1	6.1e-61	30.43	Pyruvate kinase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001697	Pyruvate kinase	PRINTS	PR01050	PYRUVTKNASE	coord: 169..185 score: 45.07 coord: 413..431 score: 51.34 coord: 304..318 score: 41.74 coord: 336..362 score: 52.14 coord: 432..448 score: 41.81 coord: 388..412 score: 60.31 coord: 363..387 score: 45.91
IPR001697	Pyruvate kinase	TIGRFAM	TIGR01064	TIGR01064	coord: 112..580 e-value: 1.4E-127 score: 424.1
IPR001697	Pyruvate kinase	PANTHER	PTHR11817	PYRUVATE KINASE	coord: 74..591
IPR036918	Pyruvate kinase, C-terminal domain superfamily	GENE3D	3.40.1380.20		coord: 455..581 e-value: 1.2E-150 score: 504.1
IPR036918	Pyruvate kinase, C-terminal domain superfamily	SUPERFAMILY	52935	PK C-terminal domain-like	coord: 443..589
IPR015793	Pyruvate kinase, barrel	PFAM	PF00224	PK	coord: 112..457 e-value: 1.8E-91 score: 306.6
IPR015795	Pyruvate kinase, C-terminal	PFAM	PF02887	PK_C	coord: 482..581 e-value: 3.2E-23 score: 82.2
IPR015806	Pyruvate kinase, insert domain superfamily	GENE3D	2.40.33.10		coord: 180..286 e-value: 1.2E-150 score: 504.1
IPR040442	Pyruvate kinase-like domain superfamily	GENE3D	3.20.20.60		coord: 112..454 e-value: 1.2E-150 score: 504.1
None	No IPR available	PANTHER	PTHR11817:SF14	PLASTIDIAL PYRUVATE KINASE 1, CHLOROPLASTIC	coord: 74..591
IPR018209	Pyruvate kinase, active site	PROSITE	PS00110	PYRUVATE_KINASE	coord: 334..346
IPR011037	Pyruvate kinase-like, insert domain superfamily	SUPERFAMILY	50800	PK beta-barrel domain-like	coord: 181..272
IPR015813	Pyruvate/Phosphoenolpyruvate kinase-like domain superfamily	SUPERFAMILY	51621	Phosphoenolpyruvate/pyruvate domain	coord: 97..458

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy5G094180.1	Chy5G094180.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006096	glycolytic process
biological_process	GO:0006629	lipid metabolic process
biological_process	GO:0010431	seed maturation
cellular_component	GO:0009570	chloroplast stroma
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016301	kinase activity
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0030955	potassium ion binding
molecular_function	GO:0004743	pyruvate kinase activity
molecular_function	GO:0003824	catalytic activity