Chy5G092360 (gene) Cucumber (hystrix) v1

Overview
NameChy5G092360
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionPectinesterase
LocationchrH05: 1506943 .. 1513938 (+)
RNA-Seq ExpressionChy5G092360
SyntenyChy5G092360
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAACTTCGGATAAATGCACTCGCTATTACAAGATTGGTAACTTTCCTAGCAACTTTTACTGCCATCTTGGCCGTATCGCCGCCGATCCCAGTGGAGAAGTCGCAAATAGCCGGGTGGTTTGCAGAAAATGTGAAGCCGTTTGCCGTTCGTAATAAGGCAGAGCTTGATCCCGCGCTGGCGACTGCAGAAGAAAACGCAACCGTAATTAAGGTAATGGGCGATGGAACGGGAAATTTCAAGACAGTCACTGAAGCTATTGCGAGCGTGCCAGCGGACAACAAGAAACGTGTAGTGATTTGGATTGGAGGAGGCGTTTATAAAGAGAAGCTTAAGATTGATAGAAATAAGGCTTTTGTTACTCTTTATGGCTCTGATCCTAAGAATATGCCTAAGTTAACTTTTGATGGCGATGCTGCTAAATACGGTACCGTTTATAGTGCTACATTGATTGTTGAGGCGGATTACTTCACTGCGGCTAATCTGATCATTGAGGTATAATTTGTTCATTATTAAATATGTTTTAAGCTTAATTAGATTAGCAAACTGGTTTCTATAATTTGAAAAAAAATAGAATTTAATTCTTTTGGTTTGATCATCCATTGTTTTCGCATTTATGTTAATTTTTTAAAAATCAAAATCTAAGAACTATTTATTATATTTGATGATATTTCATCAAACCATAAGAATTTAACCCATTAAAAAATATTAGACACAATTTAAATTTATTTCTAACTAATTATTGTAACTTTTCATAGAATGTTGATATTTTCTTGTTATTCATCTTTAGAATTACAAGTATAATTATAGACAATACAAGTGCAACATAAGGAAAGTGTAATACAAGTGCAACATAAGGAAAGTATCAATCACAAAAATATACACACACATAATTGTGGCATAATTACGGTAATATCCTCCCTCAAACTCAAGATGGTAGAGTCGACACCACCTTGAGTTTGTCAAGTAGCTGAGTGAAACGAGGAGAATGGAGAGCTTTGGTGAAAATATCTGCAGGTTGATCAGTAGTGGAGATGGATTGGAGATGGAGAGTGTTGCTCTGAAGATGATGGCGAACAAAGTGACAATCGTTCTCAATATGCTTTGTTCGTTCATGAAAGACATCATTGTGTGCAATCTGAATAGCACTGCGGTTATCACAATGAAGAATAGTGGGTGATGTCTGTGGGACTCCCATATCAGTAAGAAGCCAACGAAGCCATAATAGTTCAGAAGTAGCATCAGCTAGAGCACGATATTCAGATTCTGTGCTGGAACGGGAGACAACAGATTGTTTCTTGCTTTGCCAGGAGATAAGGGCATCACCTAAATAGAAGCAATAACCAGTTGTGGATCGTCTATCAGTAGGGTCACCTGCCCAATCAGCATCAGAGAATCCAGAGAGAACCAAAGAGGACTTGGAGGCAAATTGTAAACCGTGACCCAAAGTTCCTTTAATATAGCGAAGAATGCGAAGAACAGCAGTGAAGTGAATAGTGCGAGGAGCAGCCATGAATTGACTAACAATGTGAACTGCATATGCAATATCTGGACGGGTCACAGTTAAGTAAATTAGACTGCCAACAAGTTGTCGGTACAAAGTGGGATCGGTAAGAGGAACACCATCAAAAGGAGTGAGTCGTACATTTGGATCTAGAGGCGTTGATGATGTGGTAGAATCAGTAATACCAGATCGACTGAGGAGATCAGATGCATATTTGGCTTGAGATAAGTAGTAGCCATTAGTTGAGGATGAGATCTCAAGACCCAAAAAATAGTTGAGAGTTCCTAAGTCCTTCATCTCAAAGTGCTTACCCAGATAATATTGTAATTCAGTTATGGCTTGAGGGTCATCTCCTGTAATAATCATGTCATCAACATATAAGAGGAGAAGAACTAGACCACGAGGTGTCTGACAAGTAAATAAGGCCGAATCATGAGAGCTGGAAGTGAACCCAAGTTGGGTAATAGTGGAACTGAATGTTGCAAACCAAGCTCGAGGAGCCTGTTTGAGGCCGTAGAGAGCACGACGGAGCAGACACACTTTCTGAGGTGGCGGAGTAGTACCAGGTGGTGGTTTCATATAGACTTCTTCAGATAGTGTTCCATTGAGGAACACATTTTTGACATCCATCTGAAGGAGGGGCCATTGCTTTGCAGCTGCAATGGCCAGAAGACTGCGAACAGACGTCATTCGAGCTACAGGAGCAAACGTTTCTTCATAATCAATGCCATATTCCTGAGCATAACCTTTGGCCACAAGTCGTGCTTTGTATCGTTCAATAGAGCCATCAGAGTGTGTTTTTATTTTAAAAATCCACTTGCAACCGATAGGTTTCTTGCCAGGAGGTAAATCAACATAGTCCCAAGTATGAGCCTTTTCTAAGGCTTGGACTTCTTCATTCACTGCTTGCTGCCACAATGGGTTAGTAATGGCCTCCTTATACGAGGACGGTTCAACCAAAGACATGATAGTGGAAAAACAATGATAATCTGTAAGATGAGAAGGAGATTCCCTTACCCGGGTAGACCGTCGGACCGGTGCAGATTCAGGTTCCTCACAAGGGGCAGATGAGAGATCCGGGAGCTCAGAGATAGGATCAGATTCAGTGAGGTCAGATATGGGTAGAGGACAAGGTGTAGTGTTGGATGATGAGTCAGAGGACAAGGTGTAGTGTTGTGTTCAGTACCGTAACCCAAGAAACAACAAAGACGAGCCCTTGGTTCAAGTTTGGTATGTTCATGAGGATGTAATAATACAAAACATGCACAACCAAAGACCTTGAGATTAGAGTACGAGGGGGAAGTACTGTATAGTCGCTCAAACGGGGAAACATTATGAATGACATGAGAAGGAATGCGGTTGATGACATAGACTGAGGTGAGAGCAGCCTCTCCCCAAAATTTTTCAGGACATGAAGCAGAGAGAAGTTGAGCGCGAACAGAGTCTAGAATGTGACGGTGTTTGCGTTCAGCTCTTCCATTCTGCTGGGAGGTATGAGGACATGAGCGTTGAATCAAAGTGCCTTGATGGGCACAAAATGAAAGAAGGCGGGAGTCTTTATATTCCATCGCATTGTCAGTGCGAAGGATTTTAATTTTTCTAGAAAATTGTGTGTGAACCATATTTGCAAAATCAACATAAGTTTGATATAACGAGGAACGGTGTTTAAGAAAGTAAATCCAAGTGAAACGAGAATAGTCATCAATAAATAAGACAAAATATCGATAACCATTAACAGTAGTACAAGGAGCAGGACCCCAAATGTCAGAATGAACAAGACCAAAAGGAGTATCACATAATGAAGCGGAATTAGAAAATGATAAAGCAGGTTGTTTCGCAAGTTTGCAATTTAAACAGTCAAAAGGACTAAACTTTGAAATATTATTCAAATTGCCAGTAGAAACTAAAGTACGAATTTTGTCAGAGGAAGCATGACCTAGACGGAGATGCCACTGATATATAGTGTTATCAGTGACTGGTGCTGAGATGGGTGGGAACAGAGGAGAATGCTGAAGAGATGTGAGCTCAAATAATCGTCCCACCTTCCGTCCCGTACCAATCACTTGTCCCGTCTGAGAATCCTGAACCTGACACCCATGAGAAGAAAAGATAATGGTTAGTCCGAGATCACATAGTTGGCCAACAGAGGCTAGGTTAAATGTGAGGTTTGGAACATGATATGTGTTTGGCAGATTTATGGTGGTTGTATTAACAGATCCAATGTGAGAAATAGACATATGATTACCATCAGCTGAATGAATGGGGGGAAGGGATTGAACAGGAATAGGGGAAGTCAATAATGAAATGTCAGAAGTCATGTGATTACAACAAGCCGAATCAAGAAGCCAAGAGGTACCTGGAGTGACTGCAAGAGCAGTAGAGTTGGAAGAGATCACCTGTTTCAGTAAGTCATGCAGATTATTTAACTGAGGTGTAGTGATATCAGATGGGGTAGCAGCAACAACCACAGGGTGAGAACCAGTTTTAGAAGAAGACTTGGATTTGTAGGAGTGACCAGAAGGGCGGGGTGGGCGAGTGGGACAATTTTCCAGGATGTGACCTCGCTTGTGACAATACCTGCACTCAATAGCAGGACAATTAGCAAATTTATGTCCATGAAGTTTACAATTTTTGCAAAAAATGGTCTCAGTAGTAAGGCGTGGATGGGTGGTTGCAAGAGCGACATCAGAGGGTAGAGGAGAGACAATGCCAAGACGTTTTTCTTCAAATAAAATTTCTTGAATGGCGGCGTCGAGGGATGGCAGAGGGTTGCGATGTAAAATAGCAGCTCGAACTGATTCATACTCTGGGCGCAGGCCCATGAGGACTTTAATGAGGCGAAGATGATCTGGACTTATTTTTGCCTGGGCTAGCTGAGTCCAAATTGGCTGAAGGGTGGCAAGATACTCATTCACAGATTGTCCTCCCTCCTGATTTAGACTAACAAGGCGAGAGTGTAATTGATAATAATGAGCAAGTCCAATAGACTGAAAACGTGTGGACAGAAAATCCCAAAGATTTTTGGCAGTATCAAATGCATCAAATTGAGTATGAATTGCCGGGATAGAAGTGTTACCCAACCAAGTGATGATTTGATGATTTTTACTATCCCAGTCCTCAAGTTTTTCAATGTACTTGTCGTCCTCTGCCGAAGTGTCTTCAAGAATAACATCAGAAGGGTCTGTTTTATTTTCATCATTGGTGTCTGTACAAATTGTTTTCTTGGGAGGAGTGGGTTTGGTGATATCGCCGGTAACGATACGCCATAGCTTTCGACCAATCAGAAAGCTTCTCATTTGGTGAGCCCAAGTAATATAGTTGGAGCCATCAAGTATTGTGCTAATAGGACGAGCAATATCATGTTTCTCCATCTACACAGAAATCACAGAGCACACACCACAAAGATATAAGAAGAAACTGTGGATCTTCAGCAAGGTGAGTGAGGTTATGGATCTGGTTCAGCAACCGATTGGGAAACGTGAATATGTGCGTGACAGAAACGTGAAGTTCTTGACGGACGAGGACGAAGACGAACGTGAATCGGAGCTGCGAATGGATGCGGATGAGGACAGACGTGAATCGGAGCTGCGAACGGACGCGCGATGTGGGTTCGACAGATCTGAGTTCGTCGGATTTGATTGACGAAGACAGACGTGAATCGAAGCTGCGAGTGGACGCGGATGAGGACAGACGTGAATTGGAGCTGCGAACGGACGCGCGATCTGGGTTCGATAGATCTGAGTTCGTTGGATCTGATTGACGAAGACGGACGTGAATCGAAGCTGCGAGTGGACGCGGATGAGGACAGACGTGAATCGGAGCTGCGAACGGACGCGCGATTCGGGTTCGTCGGATTTTATTTGAACGGAGACGTTGAACGGAGACGAACTTCGTTGAATGGAGACGAACTTCTCGGCTGTGAGATCTATTCTTCAGATCTGAACAAGAGACTTGCAGACAGATCGGCGGCGGCGGCGGCGGCTATACAGCTTTACGACAGAAATCAGCGGCGACGACGGCTATACAGCTTCACGACAGAGATCGACGGCGGCGACGGCTATACAGTAACCCTAGATTAGAGGCTCTGATACCATGTAACTTTTCATAGAATGTTGATATTTTCTTGTTATTCATCTTTAGAATTACAAGTATAATTATAGACAATACAAGAAGTGTAATACAAGTGCAACATAAGGAAAGTATCAATCACAAAAATATACACACACATAATTGTGGCATAATTACGGTAATAATTATTATTTTAATATCTTATTTTCCATAGATTATCATAAATCTGGATCCAAATTGTTGAATTCTTAGTTTAAAAAAATAAATCTTTTTAAGTTTTCTAGAAAATTATAACAAATAATACCATTAGAAAAATAATATAAAAATATTGCACAATTTTAAAATATTTAAAAGAAATTAAATCCATAAATTTTTTAAAATTCTATGACTATGAATAACAACACGATAGCCACATTTTTCAAATTGAAAACTATTGCTAGCTATCGAAGGTAGTAACCGATAGCCATCTCTTAACTGTTATATATATATATATATATCAATTATTTTATTTTATGGTTGTATTATATATTGTGTTAACTTGGTTATATATTTGTTGAAAAATGGTATGAACAGAACTCATCACCAAGGCCAGATGGGGTAAGAAAAGGAGCACAAGCATTAGCGGCAAGATTTATGGGCACTAAAGCCGCAATTTACAATTGCAAATTCCTTGGATTTCAAGATACCCTTTGTGACGATGACGGTTTACATTTCTACAAAGATTGCTTCATTCAAGGCACTGTTGATTTCATTTTCGGAAAAGGAACTTCCCTTTATTTGGTATAATTTAATTATCTTATTAACATAATGTTGTTGTTGTTTTATTATTATTCTAGCTTATATAAATATTTAAATGAATATTCTAAATAATTAATTACAGAACACCCAATTGGATGTGGCTGGAGATGGAGGATTAGCAGTGATAACAGCCCATTCAAGAGAACAAGAAGCTGACACCAGTGGTTATTCGTTTGTTCATTGTAGCATTACTGGAACTGGCGGTAAAAACACTTACTTGGGAAGAGCTTGGATGCCAAGATCTAGAGTTGTCTTTGCCTACACTACAATAGCTGATATCATTCATCCCGAAGGTTGGAATGACATGAATCATGCTGGTTTCGACAAGTAAGTACTAACTTTCAAATTCTTTTTACCCTTTTTCAGACTAGGATTATATTATGCTTAGTAATGACATGAGTTTTACATTCAAAATGCATGGTGGTCAAATTGAATGCTTTTTTTCCTTGTATTTTTACAGGACGGTTATGTTCGGAGAATACAAGTGTTCAGGGCCAGGAGCGGTCTCGACGGGACGTGTAGCCTACGGCAAGCAATTGACTGATGTAGAAGCGAAGCCATTTCTTGGTCTGGAATACGTGCAAAGTGAGAAGTGGTTGCTTCCTCCTCCCAGTCCTCAAGTTTAG

mRNA sequence

ATGCAACTTCGGATAAATGCACTCGCTATTACAAGATTGGTAACTTTCCTAGCAACTTTTACTGCCATCTTGGCCGTATCGCCGCCGATCCCAGTGGAGAAGTCGCAAATAGCCGGGTGGTTTGCAGAAAATGTGAAGCCGTTTGCCGTTCGTAATAAGGCAGAGCTTGATCCCGCGCTGGCGACTGCAGAAGAAAACGCAACCGTAATTAAGGTAATGGGCGATGGAACGGGAAATTTCAAGACAGTCACTGAAGCTATTGCGAGCGTGCCAGCGGACAACAAGAAACGTGTAGTGATTTGGATTGGAGGAGGCGTTTATAAAGAGAAGCTTAAGATTGATAGAAATAAGGCTTTTGTTACTCTTTATGGCTCTGATCCTAAGAATATGCCTAAGTTAACTTTTGATGGCGATGCTGCTAAATACGGTACCGTTTATAGTGCTACATTGATTGTTGAGGCGGATTACTTCACTGCGGCTAATCTGATCATTGAGAACTCATCACCAAGGCCAGATGGGGTAAGAAAAGGAGCACAAGCATTAGCGGCAAGATTTATGGGCACTAAAGCCGCAATTTACAATTGCAAATTCCTTGGATTTCAAGATACCCTTTGTGACGATGACGGTTTACATTTCTACAAAGATTGCTTCATTCAAGGCACTGTTGATTTCATTTTCGGAAAAGGAACTTCCCTTTATTTGAACACCCAATTGGATGTGGCTGGAGATGGAGGATTAGCAGTGATAACAGCCCATTCAAGAGAACAAGAAGCTGACACCAGTGGTTATTCGTTTGTTCATTGTAGCATTACTGGAACTGGCGGTAAAAACACTTACTTGGGAAGAGCTTGGATGCCAAGATCTAGAGTTGTCTTTGCCTACACTACAATAGCTGATATCATTCATCCCGAAGGTTGGAATGACATGAATCATGCTGGTTTCGACAAGACGGTTATGTTCGGAGAATACAAGTGTTCAGGGCCAGGAGCGGTCTCGACGGGACGTGTAGCCTACGGCAAGCAATTGACTGATGTAGAAGCGAAGCCATTTCTTGGTCTGGAATACGTGCAAAGTGAGAAGTGGTTGCTTCCTCCTCCCAGTCCTCAAGTTTAG

Coding sequence (CDS)

ATGCAACTTCGGATAAATGCACTCGCTATTACAAGATTGGTAACTTTCCTAGCAACTTTTACTGCCATCTTGGCCGTATCGCCGCCGATCCCAGTGGAGAAGTCGCAAATAGCCGGGTGGTTTGCAGAAAATGTGAAGCCGTTTGCCGTTCGTAATAAGGCAGAGCTTGATCCCGCGCTGGCGACTGCAGAAGAAAACGCAACCGTAATTAAGGTAATGGGCGATGGAACGGGAAATTTCAAGACAGTCACTGAAGCTATTGCGAGCGTGCCAGCGGACAACAAGAAACGTGTAGTGATTTGGATTGGAGGAGGCGTTTATAAAGAGAAGCTTAAGATTGATAGAAATAAGGCTTTTGTTACTCTTTATGGCTCTGATCCTAAGAATATGCCTAAGTTAACTTTTGATGGCGATGCTGCTAAATACGGTACCGTTTATAGTGCTACATTGATTGTTGAGGCGGATTACTTCACTGCGGCTAATCTGATCATTGAGAACTCATCACCAAGGCCAGATGGGGTAAGAAAAGGAGCACAAGCATTAGCGGCAAGATTTATGGGCACTAAAGCCGCAATTTACAATTGCAAATTCCTTGGATTTCAAGATACCCTTTGTGACGATGACGGTTTACATTTCTACAAAGATTGCTTCATTCAAGGCACTGTTGATTTCATTTTCGGAAAAGGAACTTCCCTTTATTTGAACACCCAATTGGATGTGGCTGGAGATGGAGGATTAGCAGTGATAACAGCCCATTCAAGAGAACAAGAAGCTGACACCAGTGGTTATTCGTTTGTTCATTGTAGCATTACTGGAACTGGCGGTAAAAACACTTACTTGGGAAGAGCTTGGATGCCAAGATCTAGAGTTGTCTTTGCCTACACTACAATAGCTGATATCATTCATCCCGAAGGTTGGAATGACATGAATCATGCTGGTTTCGACAAGACGGTTATGTTCGGAGAATACAAGTGTTCAGGGCCAGGAGCGGTCTCGACGGGACGTGTAGCCTACGGCAAGCAATTGACTGATGTAGAAGCGAAGCCATTTCTTGGTCTGGAATACGTGCAAAGTGAGAAGTGGTTGCTTCCTCCTCCCAGTCCTCAAGTTTAG

Protein sequence

MQLRINALAITRLVTFLATFTAILAVSPPIPVEKSQIAGWFAENVKPFAVRNKAELDPALATAEENATVIKVMGDGTGNFKTVTEAIASVPADNKKRVVIWIGGGVYKEKLKIDRNKAFVTLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDGVRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYLNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFAYTTIADIIHPEGWNDMNHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEAKPFLGLEYVQSEKWLLPPPSPQV*
Homology
BLAST of Chy5G092360 vs. ExPASy Swiss-Prot
Match: Q84WM7 (Pectinesterase PPME1 OS=Arabidopsis thaliana OX=3702 GN=PPME1 PE=1 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 2.9e-106
Identity = 190/339 (56.05%), Postives = 235/339 (69.32%), Query Frame = 0

Query: 29  PIPVEKSQIAGWFAENVKPFAVRNKAELDPALATAEENATVIKVMGDGTGNFKTVTEAIA 88
           PIP  K Q+A WF  NV P A R    LDPAL  AE    +I V   G G FKT+T+AI 
Sbjct: 28  PIPEGKPQVAQWFNANVGPLAQRK--GLDPALVAAEAAPRIINVNPKG-GEFKTLTDAIK 87

Query: 89  SVPADNKKRVVIWIGGGVYKEKLKIDRNKAFVTLYGSDPKNMPKLTFDGDAAKYGTVYSA 148
           SVPA N KRV+I +  G YKEK+ IDRNK F+TL G  P  MP +T+DG AAKYGTV SA
Sbjct: 88  SVPAGNTKRVIIKMAPGEYKEKVTIDRNKPFITLMG-QPNAMPVITYDGTAAKYGTVDSA 147

Query: 149 TLIVEADYFTAANLIIENSSPRPDGVRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDD 208
           +LI+ +DYF A N++++N++P PDG  KGAQAL+ R  G  AA YNCKF GFQDT+CDD 
Sbjct: 148 SLIILSDYFMAVNIVVKNTAPAPDGKTKGAQALSMRISGNFAAFYNCKFYGFQDTICDDT 207

Query: 209 GLHFYKDCFIQGTVDFIFGKGTSLYLNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHC 268
           G HF+KDC+++GT DFIFG GTS+YL TQL V GD G+ VI AH+ +   + SGYSFVHC
Sbjct: 208 GNHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVVGD-GIRVIAAHAGKSAEEKSGYSFVHC 267

Query: 269 SITGTGGKNTYLGRAWMPRSRVVFAYTTIADIIHPEGWNDMNHAGFDKTVMFGEYKCSGP 328
            +TGTGG   YLGRAWM   +VV+AYT +  +++P GW +      DKTV +GEYKCSGP
Sbjct: 268 KVTGTGG-GIYLGRAWMSHPKVVYAYTEMTSVVNPTGWQENKTPAHDKTVFYGEYKCSGP 327

Query: 329 GAVSTGRVAYGKQLTDVEAKPFLGLEYVQSEKWLLPPPS 368
           G+    RV + + + D EA  FL L Y+Q  KWLLPPP+
Sbjct: 328 GSHKAKRVPFTQDIDDKEANRFLSLGYIQGSKWLLPPPA 360

BLAST of Chy5G092360 vs. ExPASy Swiss-Prot
Match: Q9LY19 (Probable pectinesterase 48 OS=Arabidopsis thaliana OX=3702 GN=PME48 PE=2 SV=2)

HSP 1 Score: 381.7 bits (979), Expect = 9.3e-105
Identity = 187/339 (55.16%), Postives = 234/339 (69.03%), Query Frame = 0

Query: 29  PIPVEKSQIAGWFAENVKPFAVRNKAELDPALATAEENATVIKVMGDGTGNFKTVTEAIA 88
           PIP  K Q+  WF  +V P A R    LDPAL  AE    +I V   G G FKT+T+AI 
Sbjct: 28  PIPEGKPQVVQWFNTHVGPLAQRK--GLDPALVAAEAAPRIINVNPKG-GEFKTLTDAIK 87

Query: 89  SVPADNKKRVVIWIGGGVYKEKLKIDRNKAFVTLYGSDPKNMPKLTFDGDAAKYGTVYSA 148
           SVPA N KRV+I +  G Y+EK+ IDRNK F+TL G  P  MP +T+DG AAKYGTV SA
Sbjct: 88  SVPAGNTKRVIIKMAHGEYREKVTIDRNKPFITLMG-QPNAMPVITYDGTAAKYGTVDSA 147

Query: 149 TLIVEADYFTAANLIIENSSPRPDGVRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDD 208
           +LI+ +DYF A N++++N++P PDG  KGAQAL+ R  G  AA YNCKF GFQDT+CDD 
Sbjct: 148 SLIILSDYFMAVNIVVKNTAPAPDGKTKGAQALSMRISGNFAAFYNCKFYGFQDTICDDT 207

Query: 209 GLHFYKDCFIQGTVDFIFGKGTSLYLNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHC 268
           G HF+KDC+++GT DFIFG GTS+YL TQL V GD G+ VI AH+ +   + SGYSFVHC
Sbjct: 208 GNHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVVGD-GIRVIAAHAGKSAEEKSGYSFVHC 267

Query: 269 SITGTGGKNTYLGRAWMPRSRVVFAYTTIADIIHPEGWNDMNHAGFDKTVMFGEYKCSGP 328
            +TGTGG   YLGRAWM   +VV+AYT +  +++P GW +      DKTV +GEYKCSGP
Sbjct: 268 KVTGTGG-GIYLGRAWMSHPKVVYAYTEMTSVVNPTGWQENKTPAHDKTVFYGEYKCSGP 327

Query: 329 GAVSTGRVAYGKQLTDVEAKPFLGLEYVQSEKWLLPPPS 368
           G+    RV + + + D EA  FL L Y+Q  KWLLPPP+
Sbjct: 328 GSHKAKRVPFTQDIDDKEANCFLSLGYIQGSKWLLPPPA 360

BLAST of Chy5G092360 vs. ExPASy Swiss-Prot
Match: Q9FKF3 (Putative pectinesterase 63 OS=Arabidopsis thaliana OX=3702 GN=PME63 PE=3 SV=2)

HSP 1 Score: 379.4 bits (973), Expect = 4.6e-104
Identity = 192/362 (53.04%), Postives = 240/362 (66.30%), Query Frame = 0

Query: 14  VTFLATFTAILAVSP--------PIPVEKSQIAGWFAENVKPFAVRNKAELDPALATAEE 73
           V+ + T   ++  SP        PIP  K +I  WF  NVK                   
Sbjct: 6   VSLIVTILLVVITSPVVFGNDAAPIPENKGRIEQWFNTNVKQ------------------ 65

Query: 74  NATVIKVMGDGTGNFKTVTEAIASVPADNKKRVVIWIGGGVYKEKLKIDRNKAFVTLYGS 133
                    +G G+FKT+TEAI SV A N +RV+I IG GVYKEK+ IDR+K F+TLYG 
Sbjct: 66  ---------NGRGHFKTITEAINSVRAGNTRRVIIKIGPGVYKEKVTIDRSKPFITLYG- 125

Query: 134 DPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDGVRKGAQALAARF 193
            P  MP LTFDG AA+YGTV SATLIV +DYF A N+I++NS+P PDG RKGAQAL+ R 
Sbjct: 126 HPNAMPVLTFDGTAAQYGTVDSATLIVLSDYFMAVNIILKNSAPMPDGKRKGAQALSMRI 185

Query: 194 MGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYLNTQLDVAGDGG 253
            G KAA YNCKF G+QDT+CDD G HF+KDC+I+GT DFIFG G SLYL TQL+V GD G
Sbjct: 186 SGNKAAFYNCKFYGYQDTICDDTGNHFFKDCYIEGTFDFIFGSGRSLYLGTQLNVVGD-G 245

Query: 254 LAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFAYTTIADIIHPEG 313
           + VITAH+ +  A+ SGYSFVHC +TGT G   YLGR+WM   +VV+AYT ++ +++P G
Sbjct: 246 IRVITAHAGKSAAEKSGYSFVHCKVTGT-GTGIYLGRSWMSHPKVVYAYTDMSSVVNPSG 305

Query: 314 WNDMNHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEAKPFLGLEYVQSEKWLLPP 368
           W +   AG DKTV +GEYKC+G G+    RV Y + + D+EAK F+ L Y+Q   WLLPP
Sbjct: 306 WQENREAGRDKTVFYGEYKCTGTGSHKEKRVKYTQDIDDIEAKYFISLGYIQGSSWLLPP 337

BLAST of Chy5G092360 vs. ExPASy Swiss-Prot
Match: B2VPR8 (Pectinesterase 2 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 9.0e-100
Identity = 182/337 (54.01%), Postives = 231/337 (68.55%), Query Frame = 0

Query: 29  PIPVEKSQIAGWFAENVKPFAVRNKAELDPALATAEENATVIKVMGDGTGNFKTVTEAIA 88
           PIP   +Q+  WF   ++P AVR KA +DPAL TAE  A VIK+  DG+G+FK++ EAI 
Sbjct: 27  PIPANSAQLNSWFDGIIQPVAVR-KATMDPALVTAEGQAKVIKLKSDGSGDFKSINEAIK 86

Query: 89  SVPADNKKRVVIWIGGGVYKEKLKIDRNKAFVTLYGSDPKNMPKLTFDGDAAKYGTVYSA 148
           S+P DN KRV++    G Y EK+KI   K ++T YG DP NMP L F G AA+YGTV SA
Sbjct: 87  SIPDDNTKRVILSFSPGNYSEKVKIGMYKHYITFYGEDPNNMPILVFGGTAAEYGTVDSA 146

Query: 149 TLIVEADYFTAANLIIENSSPRPDGVRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDD 208
           TLIVE++YF+A NL I NS+PRPDG R GAQA A R  G KA+ YN K  GFQDTLCDD 
Sbjct: 147 TLIVESNYFSAVNLKIVNSAPRPDGKRVGAQAAALRISGDKASFYNVKIYGFQDTLCDDK 206

Query: 209 GLHFYKDCFIQGTVDFIFGKGTSLYLNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHC 268
           G HFYKDC+I+GTVDFIFG G S++LNT+L        A+ITA +R+ E++ +GY FV+C
Sbjct: 207 GKHFYKDCYIEGTVDFIFGSGKSIFLNTELHAVPGDQPAIITAQARKTESEDTGYYFVNC 266

Query: 269 SITGTGGKNTYLGRAWMPRSRVVFAYTTIADIIHPEGWNDMNHAGFDKTVMFGEYKCSGP 328
            +TG G    +LGR+WMP ++VVFAYT + D IHPEGW  +     + TV F EY   GP
Sbjct: 267 RVTGGG---AFLGRSWMPAAKVVFAYTEMGDAIHPEGW-ILVKPEHESTVRFPEYNNKGP 326

Query: 329 GAVSTGRVAYGKQLTDVEAKPFLGLEYVQSEKWLLPP 366
           GA    R  + K+L+D EAK  + L  +++ KWLLPP
Sbjct: 327 GANMEKRAKFVKRLSDAEAKQSISLGSIEASKWLLPP 358

BLAST of Chy5G092360 vs. ExPASy Swiss-Prot
Match: D8VPP5 (Pectinesterase 1 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 3.4e-99
Identity = 180/337 (53.41%), Postives = 231/337 (68.55%), Query Frame = 0

Query: 29  PIPVEKSQIAGWFAENVKPFAVRNKAELDPALATAEENATVIKVMGDGTGNFKTVTEAIA 88
           PIP   +Q+  WF   ++P AVR KA +DPAL TAE    VIK+  DG+G+FK++ EAI 
Sbjct: 27  PIPSNSAQLNSWFDGIIQPVAVR-KATMDPALVTAEGQTKVIKLKSDGSGDFKSINEAIK 86

Query: 89  SVPADNKKRVVIWIGGGVYKEKLKIDRNKAFVTLYGSDPKNMPKLTFDGDAAKYGTVYSA 148
           S+P DN KRV++ +  G Y EK+KI   K ++T YG DP NMP L F G AA+YGTV SA
Sbjct: 87  SIPDDNTKRVILSLAPGNYSEKVKIGMYKHYITFYGEDPNNMPILVFGGTAAEYGTVDSA 146

Query: 149 TLIVEADYFTAANLIIENSSPRPDGVRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDD 208
           TLIVE++YF+A NL I NS+PRPDG R GAQA A R  G KA+ YN K  GFQDTLCDD 
Sbjct: 147 TLIVESNYFSAVNLKIVNSAPRPDGKRVGAQAAALRISGDKASFYNVKIYGFQDTLCDDK 206

Query: 209 GLHFYKDCFIQGTVDFIFGKGTSLYLNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHC 268
           G HFYKDC+I+GTVDFIFG G S++LNT+L        A+ITA +R+ +++ +GY FV+C
Sbjct: 207 GKHFYKDCYIEGTVDFIFGSGKSIFLNTELHAVPGDQPAIITAQARKTDSEDTGYYFVNC 266

Query: 269 SITGTGGKNTYLGRAWMPRSRVVFAYTTIADIIHPEGWNDMNHAGFDKTVMFGEYKCSGP 328
            +TG G    +LGR+WMP ++VVFAYT + D IHPEGW  +     + TV F EY   GP
Sbjct: 267 RVTGGG---AFLGRSWMPAAKVVFAYTEMVDAIHPEGW-ILVKPEHESTVRFSEYNNKGP 326

Query: 329 GAVSTGRVAYGKQLTDVEAKPFLGLEYVQSEKWLLPP 366
           GA    R  + K+L+D EAK  + L  +++ KWLLPP
Sbjct: 327 GANMEKRAKFVKRLSDAEAKQSISLGSIEASKWLLPP 358

BLAST of Chy5G092360 vs. ExPASy TrEMBL
Match: A0A0A0KEH2 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G428030 PE=4 SV=1)

HSP 1 Score: 734.6 bits (1895), Expect = 2.1e-208
Identity = 362/370 (97.84%), Postives = 363/370 (98.11%), Query Frame = 0

Query: 1   MQLRINALAITRLVTFLATFTAILAVSPPIPVEKSQIAGWFAENVKPFAVRNKAELDPAL 60
           MQ RINALAITRLVTFLATFTAILAVSPPIPVEKSQ+AGWF ENVKPFAVRNKAELDPAL
Sbjct: 1   MQPRINALAITRLVTFLATFTAILAVSPPIPVEKSQLAGWFTENVKPFAVRNKAELDPAL 60

Query: 61  ATAEENATVIKVMGDGTGNFKTVTEAIASVPADNKKRVVIWIGGGVYKEKLKIDRNKAFV 120
           ATAEENATVIKVM DGTGNFKTVTEAIASVPADNKKRVVIWIG GVYKEKLKIDRNK FV
Sbjct: 61  ATAEENATVIKVMSDGTGNFKTVTEAIASVPADNKKRVVIWIGVGVYKEKLKIDRNKPFV 120

Query: 121 TLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDGVRKGAQA 180
           TLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDGVRKGAQA
Sbjct: 121 TLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDGVRKGAQA 180

Query: 181 LAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYLNTQLDV 240
           LAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYLNTQLDV
Sbjct: 181 LAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYLNTQLDV 240

Query: 241 AGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFAYTTIADI 300
           AGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFAYTTIADI
Sbjct: 241 AGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFAYTTIADI 300

Query: 301 IHPEGWNDMNHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEAKPFLGLEYVQSEK 360
           IHPEGWNDM HAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVE KPFLGLEYVQSEK
Sbjct: 301 IHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEVKPFLGLEYVQSEK 360

Query: 361 WLLPPPSPQV 371
           WLLPPPSPQV
Sbjct: 361 WLLPPPSPQV 370

BLAST of Chy5G092360 vs. ExPASy TrEMBL
Match: A0A5A7V7V3 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G00480 PE=4 SV=1)

HSP 1 Score: 661.4 bits (1705), Expect = 2.3e-186
Identity = 326/370 (88.11%), Postives = 345/370 (93.24%), Query Frame = 0

Query: 1   MQLRINALAITRLVTFLATFTAILAVSPPIPVEKSQIAGWFAENVKPFAVRNKAELDPAL 60
           M+LR++ALAITRLVTFLATFT ILAVS PIPVEKSQI  WF++NVKP A R KAELDPAL
Sbjct: 1   MKLRMDALAITRLVTFLATFTTILAVSTPIPVEKSQIDAWFSQNVKPLADR-KAELDPAL 60

Query: 61  ATAEENATVIKVMGDGTGNFKTVTEAIASVPADNKKRVVIWIGGGVYKEKLKIDRNKAFV 120
             AEENAT+IKVM DGTG+FKT+TEAIASVPADNKKRV+I IG G+YKEKLKIDRNK FV
Sbjct: 61  VAAEENATIIKVMSDGTGDFKTITEAIASVPADNKKRVMISIGDGIYKEKLKIDRNKPFV 120

Query: 121 TLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDGVRKGAQA 180
           TLYGS PK MPKLTFDGDAAK+GTVYSATLIVEADYFTAANL+IENSSPRPDGVRKGAQA
Sbjct: 121 TLYGS-PKGMPKLTFDGDAAKFGTVYSATLIVEADYFTAANLVIENSSPRPDGVRKGAQA 180

Query: 181 LAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYLNTQLDV 240
           LAARFMGTKAAIYNCKF GFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLY+NTQLDV
Sbjct: 181 LAARFMGTKAAIYNCKFTGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYVNTQLDV 240

Query: 241 AGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFAYTTIADI 300
           AGDGGLAVITAHSREQE+D SGYSFVHCSITGTGGKNT+LGRAWMPRSRVVFAYTT+ADI
Sbjct: 241 AGDGGLAVITAHSREQESDGSGYSFVHCSITGTGGKNTFLGRAWMPRSRVVFAYTTMADI 300

Query: 301 IHPEGWNDMNHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEAKPFLGLEYVQSEK 360
           IHPEGWNDM HAGFDKTVMFGEYKCSGPG+VST RV YGKQL+D E  PFL L YVQSEK
Sbjct: 301 IHPEGWNDMKHAGFDKTVMFGEYKCSGPGSVSTERVPYGKQLSDAEVNPFLSLAYVQSEK 360

Query: 361 WLLPPPSPQV 371
           WLLPPP+PQV
Sbjct: 361 WLLPPPNPQV 368

BLAST of Chy5G092360 vs. ExPASy TrEMBL
Match: A0A1S4DXM9 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103491454 PE=4 SV=1)

HSP 1 Score: 655.6 bits (1690), Expect = 1.2e-184
Identity = 323/366 (88.25%), Postives = 341/366 (93.17%), Query Frame = 0

Query: 5   INALAITRLVTFLATFTAILAVSPPIPVEKSQIAGWFAENVKPFAVRNKAELDPALATAE 64
           ++ALAITRLVTFLATFT ILAVS PIPVEKSQI  WF++NVKP A R KAELDPAL  AE
Sbjct: 1   MDALAITRLVTFLATFTTILAVSTPIPVEKSQIDAWFSQNVKPLADR-KAELDPALVAAE 60

Query: 65  ENATVIKVMGDGTGNFKTVTEAIASVPADNKKRVVIWIGGGVYKEKLKIDRNKAFVTLYG 124
           ENAT+IKVM DGTG+FKT+TEAIASVPADNKKRV+I IG G+YKEKLKIDRNK FVTLYG
Sbjct: 61  ENATIIKVMSDGTGDFKTITEAIASVPADNKKRVMISIGDGIYKEKLKIDRNKPFVTLYG 120

Query: 125 SDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDGVRKGAQALAAR 184
           S PK MPKLTFDGDAAK+GTVYSATLIVEADYFTAANL+IENSSPRPDGVRKGAQALAAR
Sbjct: 121 S-PKGMPKLTFDGDAAKFGTVYSATLIVEADYFTAANLVIENSSPRPDGVRKGAQALAAR 180

Query: 185 FMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYLNTQLDVAGDG 244
           FMGTKAAIYNCKF GFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLY+NTQLDVAGDG
Sbjct: 181 FMGTKAAIYNCKFTGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYVNTQLDVAGDG 240

Query: 245 GLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFAYTTIADIIHPE 304
           GLAVITAHSREQE+D SGYSFVHCSITGTGGKNT+LGRAWMPRSRVVFAYTT+ADIIHPE
Sbjct: 241 GLAVITAHSREQESDGSGYSFVHCSITGTGGKNTFLGRAWMPRSRVVFAYTTMADIIHPE 300

Query: 305 GWNDMNHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEAKPFLGLEYVQSEKWLLP 364
           GWNDM HAGFDKTVMFGEYKCSGPG+VST RV YGKQL+D E  PFL L YVQSEKWLLP
Sbjct: 301 GWNDMKHAGFDKTVMFGEYKCSGPGSVSTERVPYGKQLSDAEVNPFLSLAYVQSEKWLLP 360

Query: 365 PPSPQV 371
           PP+PQV
Sbjct: 361 PPNPQV 364

BLAST of Chy5G092360 vs. ExPASy TrEMBL
Match: A0A0A0KI78 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G428290 PE=4 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 1.1e-169
Identity = 297/370 (80.27%), Postives = 332/370 (89.73%), Query Frame = 0

Query: 1   MQLRINALAITRLVTFLATFTAILAV-SPPIPVEKSQIAGWFAENVKPFAVRNKAELDPA 60
           M+LR++ALAIT LVTFLATFTAILAV SP +PV+K+Q+  WF+ENVKPFA RNKAELDPA
Sbjct: 1   MKLRMDALAITTLVTFLATFTAILAVPSPMVPVDKAQLEAWFSENVKPFADRNKAELDPA 60

Query: 61  LATAEENATVIKVMGDGTGNFKTVTEAIASVPADNKKRVVIWIGGGVYKEKLKIDRNKAF 120
           +  AE N T++KVM DGTG+FKTVTEAIASVP +NK RVVIWIG GVYKEKL I++NK F
Sbjct: 61  VVAAEGNVTIVKVMSDGTGDFKTVTEAIASVPVNNKNRVVIWIGEGVYKEKLTIEKNKPF 120

Query: 121 VTLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDGVRKGAQ 180
           +TL G+ PKN+P LTFDG A+KYGTVYSATLIVEADYF AANLIIEN+SPRP+G RK AQ
Sbjct: 121 ITLCGT-PKNVPTLTFDGVASKYGTVYSATLIVEADYFVAANLIIENTSPRPNG-RKEAQ 180

Query: 181 ALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYLNTQLD 240
           ALAARF GTK+A YNCKFLGFQDTLCDDDGLH YKDCFIQGTVDF+FGKGTSLYLNT+L+
Sbjct: 181 ALAARFRGTKSAFYNCKFLGFQDTLCDDDGLHLYKDCFIQGTVDFVFGKGTSLYLNTELN 240

Query: 241 VAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFAYTTIAD 300
           V G+G  AVITAHSREQEAD SGYSFVHCSITG  GK+T+LGRAWMPRSRV+FAYT++ D
Sbjct: 241 VVGEGQFAVITAHSREQEADASGYSFVHCSITG-NGKDTFLGRAWMPRSRVIFAYTSMID 300

Query: 301 IIHPEGWNDMNHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEAKPFLGLEYVQSE 360
           IIHPEGWNDM HAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLT+ E KP+L LE+VQS 
Sbjct: 301 IIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTEAEVKPYLSLEFVQSA 360

Query: 361 KWLLPPPSPQ 370
           KWLLPPP+PQ
Sbjct: 361 KWLLPPPNPQ 367

BLAST of Chy5G092360 vs. ExPASy TrEMBL
Match: A0A1S3BMG4 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103491453 PE=4 SV=1)

HSP 1 Score: 602.8 bits (1553), Expect = 9.5e-169
Identity = 292/370 (78.92%), Postives = 320/370 (86.49%), Query Frame = 0

Query: 1   MQLRINALAITRLVTFLATFTAILAVSPPIPVEKSQIAGWFAENVKPFAVRNKAELDPAL 60
           M+  INAL +TR + FL  F AILA SP IP EKSQ+  W+ ENVK F  RNKAELDP L
Sbjct: 1   MKYCINALRLTRWIMFLEAFAAILAASPLIPTEKSQLDEWYWENVKAFGNRNKAELDPEL 60

Query: 61  ATAEENATVIKVMGDGTGNFKTVTEAIASVPADNKKRVVIWIGGGVYKEKLKIDRNKAFV 120
             AEE+ATVIKV GDG+G+FKT+ EAI SVP  N KR+VIWIGGGVYKEKLKIDRNK FV
Sbjct: 61  VAAEESATVIKVRGDGSGDFKTIAEAIESVPIGNTKRIVIWIGGGVYKEKLKIDRNKPFV 120

Query: 121 TLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDGVRKGAQA 180
           TLYGS P NMP LTFDGDA+KYGTVYSATLIVEADYFTAANL+IENSSPRPDGVRKGAQA
Sbjct: 121 TLYGS-PNNMPNLTFDGDASKYGTVYSATLIVEADYFTAANLVIENSSPRPDGVRKGAQA 180

Query: 181 LAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYLNTQLDV 240
           LAAR  G KAAIYNCKF+GFQDTLCDDDGLH YKDCFIQGTVDFIFGKGTSLYLNTQLDV
Sbjct: 181 LAARIRGNKAAIYNCKFIGFQDTLCDDDGLHLYKDCFIQGTVDFIFGKGTSLYLNTQLDV 240

Query: 241 AGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFAYTTIADI 300
            G+GGL VI AHSR+QE+D SGYSFVHCSITGTGG+NTYLGRAW PRSRVVFAYTT+ADI
Sbjct: 241 VGNGGLGVIAAHSRDQESDGSGYSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTTMADI 300

Query: 301 IHPEGWNDMNHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEAKPFLGLEYVQSEK 360
           IHPEGWNDMNH G+DKTV+FGEYKC GPG+ S+ RV Y KQL+  E +PF+ L YVQSEK
Sbjct: 301 IHPEGWNDMNHFGYDKTVLFGEYKCWGPGSKSSERVKYSKQLSYEEVEPFVSLGYVQSEK 360

Query: 361 WLLPPPSPQV 371
           WLLPPP+P++
Sbjct: 361 WLLPPPNPEI 369

BLAST of Chy5G092360 vs. NCBI nr
Match: XP_011657599.1 (putative pectinesterase 63 [Cucumis sativus])

HSP 1 Score: 729 bits (1882), Expect = 4.52e-265
Identity = 362/370 (97.84%), Postives = 363/370 (98.11%), Query Frame = 0

Query: 1   MQLRINALAITRLVTFLATFTAILAVSPPIPVEKSQIAGWFAENVKPFAVRNKAELDPAL 60
           MQ RINALAITRLVTFLATFTAILAVSPPIPVEKSQ+AGWF ENVKPFAVRNKAELDPAL
Sbjct: 1   MQPRINALAITRLVTFLATFTAILAVSPPIPVEKSQLAGWFTENVKPFAVRNKAELDPAL 60

Query: 61  ATAEENATVIKVMGDGTGNFKTVTEAIASVPADNKKRVVIWIGGGVYKEKLKIDRNKAFV 120
           ATAEENATVIKVM DGTGNFKTVTEAIASVPADNKKRVVIWIG GVYKEKLKIDRNK FV
Sbjct: 61  ATAEENATVIKVMSDGTGNFKTVTEAIASVPADNKKRVVIWIGVGVYKEKLKIDRNKPFV 120

Query: 121 TLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDGVRKGAQA 180
           TLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDGVRKGAQA
Sbjct: 121 TLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDGVRKGAQA 180

Query: 181 LAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYLNTQLDV 240
           LAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYLNTQLDV
Sbjct: 181 LAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYLNTQLDV 240

Query: 241 AGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFAYTTIADI 300
           AGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFAYTTIADI
Sbjct: 241 AGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFAYTTIADI 300

Query: 301 IHPEGWNDMNHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEAKPFLGLEYVQSEK 360
           IHPEGWNDM HAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVE KPFLGLEYVQSEK
Sbjct: 301 IHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEVKPFLGLEYVQSEK 360

Query: 361 WLLPPPSPQV 370
           WLLPPPSPQV
Sbjct: 361 WLLPPPSPQV 370

BLAST of Chy5G092360 vs. NCBI nr
Match: KAA0061749.1 (pectinesterase PPME1-like [Cucumis melo var. makuwa] >TYJ96089.1 pectinesterase PPME1-like [Cucumis melo var. makuwa])

HSP 1 Score: 656 bits (1692), Expect = 3.78e-236
Identity = 326/370 (88.11%), Postives = 345/370 (93.24%), Query Frame = 0

Query: 1   MQLRINALAITRLVTFLATFTAILAVSPPIPVEKSQIAGWFAENVKPFAVRNKAELDPAL 60
           M+LR++ALAITRLVTFLATFT ILAVS PIPVEKSQI  WF++NVKP A R KAELDPAL
Sbjct: 1   MKLRMDALAITRLVTFLATFTTILAVSTPIPVEKSQIDAWFSQNVKPLADR-KAELDPAL 60

Query: 61  ATAEENATVIKVMGDGTGNFKTVTEAIASVPADNKKRVVIWIGGGVYKEKLKIDRNKAFV 120
             AEENAT+IKVM DGTG+FKT+TEAIASVPADNKKRV+I IG G+YKEKLKIDRNK FV
Sbjct: 61  VAAEENATIIKVMSDGTGDFKTITEAIASVPADNKKRVMISIGDGIYKEKLKIDRNKPFV 120

Query: 121 TLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDGVRKGAQA 180
           TLYGS PK MPKLTFDGDAAK+GTVYSATLIVEADYFTAANL+IENSSPRPDGVRKGAQA
Sbjct: 121 TLYGS-PKGMPKLTFDGDAAKFGTVYSATLIVEADYFTAANLVIENSSPRPDGVRKGAQA 180

Query: 181 LAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYLNTQLDV 240
           LAARFMGTKAAIYNCKF GFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLY+NTQLDV
Sbjct: 181 LAARFMGTKAAIYNCKFTGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYVNTQLDV 240

Query: 241 AGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFAYTTIADI 300
           AGDGGLAVITAHSREQE+D SGYSFVHCSITGTGGKNT+LGRAWMPRSRVVFAYTT+ADI
Sbjct: 241 AGDGGLAVITAHSREQESDGSGYSFVHCSITGTGGKNTFLGRAWMPRSRVVFAYTTMADI 300

Query: 301 IHPEGWNDMNHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEAKPFLGLEYVQSEK 360
           IHPEGWNDM HAGFDKTVMFGEYKCSGPG+VST RV YGKQL+D E  PFL L YVQSEK
Sbjct: 301 IHPEGWNDMKHAGFDKTVMFGEYKCSGPGSVSTERVPYGKQLSDAEVNPFLSLAYVQSEK 360

Query: 361 WLLPPPSPQV 370
           WLLPPP+PQV
Sbjct: 361 WLLPPPNPQV 368

BLAST of Chy5G092360 vs. NCBI nr
Match: XP_016900732.1 (PREDICTED: pectinesterase PPME1-like [Cucumis melo])

HSP 1 Score: 650 bits (1678), Expect = 4.42e-234
Identity = 323/365 (88.49%), Postives = 340/365 (93.15%), Query Frame = 0

Query: 6   NALAITRLVTFLATFTAILAVSPPIPVEKSQIAGWFAENVKPFAVRNKAELDPALATAEE 65
           +ALAITRLVTFLATFT ILAVS PIPVEKSQI  WF++NVKP A R KAELDPAL  AEE
Sbjct: 2   DALAITRLVTFLATFTTILAVSTPIPVEKSQIDAWFSQNVKPLADR-KAELDPALVAAEE 61

Query: 66  NATVIKVMGDGTGNFKTVTEAIASVPADNKKRVVIWIGGGVYKEKLKIDRNKAFVTLYGS 125
           NAT+IKVM DGTG+FKT+TEAIASVPADNKKRV+I IG G+YKEKLKIDRNK FVTLYGS
Sbjct: 62  NATIIKVMSDGTGDFKTITEAIASVPADNKKRVMISIGDGIYKEKLKIDRNKPFVTLYGS 121

Query: 126 DPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDGVRKGAQALAARF 185
            PK MPKLTFDGDAAK+GTVYSATLIVEADYFTAANL+IENSSPRPDGVRKGAQALAARF
Sbjct: 122 -PKGMPKLTFDGDAAKFGTVYSATLIVEADYFTAANLVIENSSPRPDGVRKGAQALAARF 181

Query: 186 MGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYLNTQLDVAGDGG 245
           MGTKAAIYNCKF GFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLY+NTQLDVAGDGG
Sbjct: 182 MGTKAAIYNCKFTGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYVNTQLDVAGDGG 241

Query: 246 LAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFAYTTIADIIHPEG 305
           LAVITAHSREQE+D SGYSFVHCSITGTGGKNT+LGRAWMPRSRVVFAYTT+ADIIHPEG
Sbjct: 242 LAVITAHSREQESDGSGYSFVHCSITGTGGKNTFLGRAWMPRSRVVFAYTTMADIIHPEG 301

Query: 306 WNDMNHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEAKPFLGLEYVQSEKWLLPP 365
           WNDM HAGFDKTVMFGEYKCSGPG+VST RV YGKQL+D E  PFL L YVQSEKWLLPP
Sbjct: 302 WNDMKHAGFDKTVMFGEYKCSGPGSVSTERVPYGKQLSDAEVNPFLSLAYVQSEKWLLPP 361

Query: 366 PSPQV 370
           P+PQV
Sbjct: 362 PNPQV 364

BLAST of Chy5G092360 vs. NCBI nr
Match: XP_038902684.1 (putative pectinesterase 63 [Benincasa hispida])

HSP 1 Score: 620 bits (1600), Expect = 3.91e-222
Identity = 303/370 (81.89%), Postives = 332/370 (89.73%), Query Frame = 0

Query: 1   MQLRINALAITRLVTFLATFTAILAVSPPIPVEKSQIAGWFAENVKPFAVRNKAELDPAL 60
           M+  I+AL +TRLV FLA F AI AVSPP+P EKSQ+ GWF+ENVKPFA   KAELDPAL
Sbjct: 1   MKYCIDALGLTRLVIFLAAFAAIFAVSPPVPAEKSQLEGWFSENVKPFA-DCKAELDPAL 60

Query: 61  ATAEENATVIKVMGDGTGNFKTVTEAIASVPADNKKRVVIWIGGGVYKEKLKIDRNKAFV 120
             AEENATV+KV  DG+G+FKTVTEAI SVPA N +RVVIWIG GVYKEKLKIDRNK FV
Sbjct: 61  VAAEENATVVKVRADGSGDFKTVTEAINSVPAGNTRRVVIWIGEGVYKEKLKIDRNKPFV 120

Query: 121 TLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDGVRKGAQA 180
           TLYGS P NMP LTFDGDAAKYGTVYSATLIVE+DYF AANL+IENSSPRPDG+RKGAQA
Sbjct: 121 TLYGS-PNNMPNLTFDGDAAKYGTVYSATLIVESDYFVAANLVIENSSPRPDGIRKGAQA 180

Query: 181 LAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYLNTQLDV 240
           LAAR MGTKAAIYNCKF+GFQDTLCDD+GLH YKDCFIQGTVDFIFGKGTSLYLN++LDV
Sbjct: 181 LAARLMGTKAAIYNCKFIGFQDTLCDDEGLHLYKDCFIQGTVDFIFGKGTSLYLNSRLDV 240

Query: 241 AGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFAYTTIADI 300
            GDGGLAVITAHSRE+E+D+SGYSF HCSITGTGGKNTYLGRAWMPRSRVVFAYTT+ADI
Sbjct: 241 VGDGGLAVITAHSREEESDSSGYSFAHCSITGTGGKNTYLGRAWMPRSRVVFAYTTMADI 300

Query: 301 IHPEGWNDMNHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEAKPFLGLEYVQSEK 360
           IH EGWNDMNH+G+D TV+FGEYKCSGPGA  +GRV Y KQL+DVE KPF+ L Y+QSEK
Sbjct: 301 IHAEGWNDMNHSGYDATVLFGEYKCSGPGAELSGRVKYSKQLSDVEVKPFISLGYLQSEK 360

Query: 361 WLLPPPSPQV 370
           WLLPPP+PQV
Sbjct: 361 WLLPPPNPQV 368

BLAST of Chy5G092360 vs. NCBI nr
Match: KGN48350.2 (hypothetical protein Csa_003568 [Cucumis sativus])

HSP 1 Score: 613 bits (1582), Expect = 2.16e-219
Identity = 305/371 (82.21%), Postives = 336/371 (90.57%), Query Frame = 0

Query: 1   MQLRINALAITRLVTFLATFTAILAV-SPPIPVEKSQIAGWFAENVKPFAVRNKAELDPA 60
           M+LR++ALAIT LVTFLATFTAILAV SP +PV+K+Q+  WF+ENVKPFA RNKAELDPA
Sbjct: 1   MKLRMDALAITTLVTFLATFTAILAVPSPMVPVDKAQLEAWFSENVKPFADRNKAELDPA 60

Query: 61  LATAEENATVIKVMGDGTGNFKTVTEAIASVPADNKKRVVIWIGGGVYKEKLKIDRNKAF 120
           +  AE N T++KVM DGTG+FKTVTEAIASVP +NK RVVIWIG GVYKEKL I++NK F
Sbjct: 61  VVAAEGNVTIVKVMSDGTGDFKTVTEAIASVPVNNKNRVVIWIGEGVYKEKLTIEKNKPF 120

Query: 121 VTLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDGVRKGAQ 180
           +TL G+ PKN+P LTFDG A+KYGTVYSATLIVEADYF AANLIIEN+SPRP+G RK AQ
Sbjct: 121 ITLCGT-PKNVPTLTFDGVASKYGTVYSATLIVEADYFVAANLIIENTSPRPNG-RKEAQ 180

Query: 181 ALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYLNTQLD 240
           ALAARF GTK+A YNCKFLGFQDTLCDDDGLH YKDCFIQGTVDF+FGKGTSLYLNT+L+
Sbjct: 181 ALAARFRGTKSAFYNCKFLGFQDTLCDDDGLHLYKDCFIQGTVDFVFGKGTSLYLNTELN 240

Query: 241 VAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFAYTTIAD 300
           V G+G  AVITAHSREQEAD SGYSFVHCSITG G K+T+LGRAWMPRSRV+FAYT++ D
Sbjct: 241 VVGEGQFAVITAHSREQEADASGYSFVHCSITGNG-KDTFLGRAWMPRSRVIFAYTSMID 300

Query: 301 IIHPEGWNDMNHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEAKPFLGLEYVQSE 360
           IIHPEGWNDM HAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVE KPFLGLEYVQSE
Sbjct: 301 IIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEVKPFLGLEYVQSE 360

Query: 361 KWLLPPPSPQV 370
           KWLLPPPSPQV
Sbjct: 361 KWLLPPPSPQV 368

BLAST of Chy5G092360 vs. TAIR 10
Match: AT1G69940.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 386.7 bits (992), Expect = 2.1e-107
Identity = 190/339 (56.05%), Postives = 235/339 (69.32%), Query Frame = 0

Query: 29  PIPVEKSQIAGWFAENVKPFAVRNKAELDPALATAEENATVIKVMGDGTGNFKTVTEAIA 88
           PIP  K Q+A WF  NV P A R    LDPAL  AE    +I V   G G FKT+T+AI 
Sbjct: 28  PIPEGKPQVAQWFNANVGPLAQRK--GLDPALVAAEAAPRIINVNPKG-GEFKTLTDAIK 87

Query: 89  SVPADNKKRVVIWIGGGVYKEKLKIDRNKAFVTLYGSDPKNMPKLTFDGDAAKYGTVYSA 148
           SVPA N KRV+I +  G YKEK+ IDRNK F+TL G  P  MP +T+DG AAKYGTV SA
Sbjct: 88  SVPAGNTKRVIIKMAPGEYKEKVTIDRNKPFITLMG-QPNAMPVITYDGTAAKYGTVDSA 147

Query: 149 TLIVEADYFTAANLIIENSSPRPDGVRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDD 208
           +LI+ +DYF A N++++N++P PDG  KGAQAL+ R  G  AA YNCKF GFQDT+CDD 
Sbjct: 148 SLIILSDYFMAVNIVVKNTAPAPDGKTKGAQALSMRISGNFAAFYNCKFYGFQDTICDDT 207

Query: 209 GLHFYKDCFIQGTVDFIFGKGTSLYLNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHC 268
           G HF+KDC+++GT DFIFG GTS+YL TQL V GD G+ VI AH+ +   + SGYSFVHC
Sbjct: 208 GNHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVVGD-GIRVIAAHAGKSAEEKSGYSFVHC 267

Query: 269 SITGTGGKNTYLGRAWMPRSRVVFAYTTIADIIHPEGWNDMNHAGFDKTVMFGEYKCSGP 328
            +TGTGG   YLGRAWM   +VV+AYT +  +++P GW +      DKTV +GEYKCSGP
Sbjct: 268 KVTGTGG-GIYLGRAWMSHPKVVYAYTEMTSVVNPTGWQENKTPAHDKTVFYGEYKCSGP 327

Query: 329 GAVSTGRVAYGKQLTDVEAKPFLGLEYVQSEKWLLPPPS 368
           G+    RV + + + D EA  FL L Y+Q  KWLLPPP+
Sbjct: 328 GSHKAKRVPFTQDIDDKEANRFLSLGYIQGSKWLLPPPA 360

BLAST of Chy5G092360 vs. TAIR 10
Match: AT5G07410.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 381.7 bits (979), Expect = 6.6e-106
Identity = 187/339 (55.16%), Postives = 234/339 (69.03%), Query Frame = 0

Query: 29  PIPVEKSQIAGWFAENVKPFAVRNKAELDPALATAEENATVIKVMGDGTGNFKTVTEAIA 88
           PIP  K Q+  WF  +V P A R    LDPAL  AE    +I V   G G FKT+T+AI 
Sbjct: 28  PIPEGKPQVVQWFNTHVGPLAQRK--GLDPALVAAEAAPRIINVNPKG-GEFKTLTDAIK 87

Query: 89  SVPADNKKRVVIWIGGGVYKEKLKIDRNKAFVTLYGSDPKNMPKLTFDGDAAKYGTVYSA 148
           SVPA N KRV+I +  G Y+EK+ IDRNK F+TL G  P  MP +T+DG AAKYGTV SA
Sbjct: 88  SVPAGNTKRVIIKMAHGEYREKVTIDRNKPFITLMG-QPNAMPVITYDGTAAKYGTVDSA 147

Query: 149 TLIVEADYFTAANLIIENSSPRPDGVRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDD 208
           +LI+ +DYF A N++++N++P PDG  KGAQAL+ R  G  AA YNCKF GFQDT+CDD 
Sbjct: 148 SLIILSDYFMAVNIVVKNTAPAPDGKTKGAQALSMRISGNFAAFYNCKFYGFQDTICDDT 207

Query: 209 GLHFYKDCFIQGTVDFIFGKGTSLYLNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHC 268
           G HF+KDC+++GT DFIFG GTS+YL TQL V GD G+ VI AH+ +   + SGYSFVHC
Sbjct: 208 GNHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVVGD-GIRVIAAHAGKSAEEKSGYSFVHC 267

Query: 269 SITGTGGKNTYLGRAWMPRSRVVFAYTTIADIIHPEGWNDMNHAGFDKTVMFGEYKCSGP 328
            +TGTGG   YLGRAWM   +VV+AYT +  +++P GW +      DKTV +GEYKCSGP
Sbjct: 268 KVTGTGG-GIYLGRAWMSHPKVVYAYTEMTSVVNPTGWQENKTPAHDKTVFYGEYKCSGP 327

Query: 329 GAVSTGRVAYGKQLTDVEAKPFLGLEYVQSEKWLLPPPS 368
           G+    RV + + + D EA  FL L Y+Q  KWLLPPP+
Sbjct: 328 GSHKAKRVPFTQDIDDKEANCFLSLGYIQGSKWLLPPPA 360

BLAST of Chy5G092360 vs. TAIR 10
Match: AT5G61680.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 379.4 bits (973), Expect = 3.3e-105
Identity = 192/362 (53.04%), Postives = 240/362 (66.30%), Query Frame = 0

Query: 14  VTFLATFTAILAVSP--------PIPVEKSQIAGWFAENVKPFAVRNKAELDPALATAEE 73
           V+ + T   ++  SP        PIP  K +I  WF  NVK                   
Sbjct: 6   VSLIVTILLVVITSPVVFGNDAAPIPENKGRIEQWFNTNVKQ------------------ 65

Query: 74  NATVIKVMGDGTGNFKTVTEAIASVPADNKKRVVIWIGGGVYKEKLKIDRNKAFVTLYGS 133
                    +G G+FKT+TEAI SV A N +RV+I IG GVYKEK+ IDR+K F+TLYG 
Sbjct: 66  ---------NGRGHFKTITEAINSVRAGNTRRVIIKIGPGVYKEKVTIDRSKPFITLYG- 125

Query: 134 DPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDGVRKGAQALAARF 193
            P  MP LTFDG AA+YGTV SATLIV +DYF A N+I++NS+P PDG RKGAQAL+ R 
Sbjct: 126 HPNAMPVLTFDGTAAQYGTVDSATLIVLSDYFMAVNIILKNSAPMPDGKRKGAQALSMRI 185

Query: 194 MGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYLNTQLDVAGDGG 253
            G KAA YNCKF G+QDT+CDD G HF+KDC+I+GT DFIFG G SLYL TQL+V GD G
Sbjct: 186 SGNKAAFYNCKFYGYQDTICDDTGNHFFKDCYIEGTFDFIFGSGRSLYLGTQLNVVGD-G 245

Query: 254 LAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFAYTTIADIIHPEG 313
           + VITAH+ +  A+ SGYSFVHC +TGT G   YLGR+WM   +VV+AYT ++ +++P G
Sbjct: 246 IRVITAHAGKSAAEKSGYSFVHCKVTGT-GTGIYLGRSWMSHPKVVYAYTDMSSVVNPSG 305

Query: 314 WNDMNHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEAKPFLGLEYVQSEKWLLPP 368
           W +   AG DKTV +GEYKC+G G+    RV Y + + D+EAK F+ L Y+Q   WLLPP
Sbjct: 306 WQENREAGRDKTVFYGEYKCTGTGSHKEKRVKYTQDIDDIEAKYFISLGYIQGSSWLLPP 337

BLAST of Chy5G092360 vs. TAIR 10
Match: AT5G07430.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 352.8 bits (904), Expect = 3.3e-97
Identity = 184/357 (51.54%), Postives = 236/357 (66.11%), Query Frame = 0

Query: 13  LVTFLATFTA--ILAV-SPPIPVEKSQIAGWFAENVKPFAVRNKAELDPALATAEENATV 72
           +V FL  F +  +LA  + PIP  ++QI  WF  NVKP++ R K  LDPAL  AE    +
Sbjct: 8   VVAFLVVFASPVVLATDTDPIPENRAQIPQWFKTNVKPYSQR-KGTLDPALEAAEAARQI 67

Query: 73  IKVMGDGTGNFKTVTEAIASVPADNKKRVVIWIGGGVYKEKLKIDRNKAFVTLYGSDPKN 132
           I V   G  NFKT+ EAI S+P  NK RV+I +  GVY EK+ ID  + F+TL G  P  
Sbjct: 68  ITVNQKGGANFKTLNEAIKSIPTGNKNRVIIKLAPGVYNEKVTIDIARPFITLLG-QPGA 127

Query: 133 MPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDGVRKGAQALAARFMGTK 192
              LT+ G AA+YGTV SATLIV A+YF AA+L I+N++P P    +G QALA R    K
Sbjct: 128 ETVLTYHGTAAQYGTVESATLIVWAEYFQAAHLTIKNTAPMPKPGSQG-QALAMRINADK 187

Query: 193 AAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYLNTQLDVAGDGGLAVI 252
           AA Y+C+F GFQDTLCDD G HF+KDC+I+GT DFIFG+G SLYLNTQL   GD GL VI
Sbjct: 188 AAFYSCRFHGFQDTLCDDKGNHFFKDCYIEGTYDFIFGRGASLYLNTQLHAVGD-GLRVI 247

Query: 253 TAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFAYTTIADIIHPEGWNDM 312
           TA  R+   + +GY+FVHC +TGT G   YLGR+WM   +VV+A+T +  +++P GW + 
Sbjct: 248 TAQGRQSATEQNGYTFVHCKVTGT-GTGIYLGRSWMSHPKVVYAFTEMTSVVNPSGWREN 307

Query: 313 NHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEAKPFLGLEYVQSEKWLLPPP 367
            + G+DKTV +GEYKC GPG+    RV Y + +   E  PFL L Y++   WLLPPP
Sbjct: 308 LNRGYDKTVFYGEYKCFGPGSHLEKRVPYTQDIDKNEVTPFLTLGYIKGSTWLLPPP 359

BLAST of Chy5G092360 vs. TAIR 10
Match: AT5G07420.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 352.8 bits (904), Expect = 3.3e-97
Identity = 182/361 (50.42%), Postives = 237/361 (65.65%), Query Frame = 0

Query: 7   ALAITRLVTFLATFTAILAVSPPIPVEKSQIAGWFAENVKPFAVRNKAELDPALATAEEN 66
           +LA+  L+ F A+   +     PIP +++QI  WF  NVKPF+ R +  LDP L  AE +
Sbjct: 5   SLALVALLVFFASPVVLADDITPIPADRAQIPQWFMANVKPFSQR-RGTLDPELEAAEAS 64

Query: 67  ATVIKVMGDGTGNFKTVTEAIASVPADNKKRVVIWIGGGVYKEKLKIDRNKAFVTLYGSD 126
             VI V  +G G+FKT+  AI S+P  NK RV+I +  G+Y EK+ +D  + +VTL G  
Sbjct: 65  RRVIIVNQNGGGDFKTINAAIKSIPLANKNRVIIKLAPGIYHEKVTVDVGRPYVTLLGK- 124

Query: 127 PKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDGVRKGAQALAARFM 186
           P     LT+ G AAKYGTV SATLIV A  F AANL I N+SP P    +G QALA R  
Sbjct: 125 PGAETNLTYAGTAAKYGTVESATLIVWATNFLAANLNIINTSPMPKPGTQG-QALAMRIN 184

Query: 187 GTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYLNTQLDVAGDGGL 246
           G KAA YNC+F GFQDTLCDD G HF+K+C+I+GT DFIFG+G SLYL TQL   GD GL
Sbjct: 185 GDKAAFYNCRFYGFQDTLCDDRGNHFFKNCYIEGTYDFIFGRGASLYLTTQLHAVGD-GL 244

Query: 247 AVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFAYTTIADIIHPEGW 306
            VI AH+R+   + +GYSFVHC +TG  G   YLGRAWM   +VV++YT ++ +++P GW
Sbjct: 245 RVIAAHNRQSTTEQNGYSFVHCKVTGV-GTGIYLGRAWMSHPKVVYSYTEMSSVVNPSGW 304

Query: 307 NDMNHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEAKPFLGLEYVQSEKWLLPPP 366
            +      DKTV +GEY C+GPG+    RVA+ + + + EA  FL L Y++  KWLLPPP
Sbjct: 305 QENRVRAHDKTVFYGEYMCTGPGSHKAKRVAHTQDIDNKEASQFLTLGYIKGSKWLLPPP 360

Query: 367 S 368
           +
Sbjct: 365 A 360

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84WM72.9e-10656.05Pectinesterase PPME1 OS=Arabidopsis thaliana OX=3702 GN=PPME1 PE=1 SV=1[more]
Q9LY199.3e-10555.16Probable pectinesterase 48 OS=Arabidopsis thaliana OX=3702 GN=PME48 PE=2 SV=2[more]
Q9FKF34.6e-10453.04Putative pectinesterase 63 OS=Arabidopsis thaliana OX=3702 GN=PME63 PE=3 SV=2[more]
B2VPR89.0e-10054.01Pectinesterase 2 OS=Olea europaea OX=4146 PE=1 SV=1[more]
D8VPP53.4e-9953.41Pectinesterase 1 OS=Olea europaea OX=4146 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KEH22.1e-20897.84Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G428030 PE=4 SV=1[more]
A0A5A7V7V32.3e-18688.11Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G00480... [more]
A0A1S4DXM91.2e-18488.25Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103491454 PE=4 SV=1[more]
A0A0A0KI781.1e-16980.27Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G428290 PE=4 SV=1[more]
A0A1S3BMG49.5e-16978.92Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103491453 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_011657599.14.52e-26597.84putative pectinesterase 63 [Cucumis sativus][more]
KAA0061749.13.78e-23688.11pectinesterase PPME1-like [Cucumis melo var. makuwa] >TYJ96089.1 pectinesterase ... [more]
XP_016900732.14.42e-23488.49PREDICTED: pectinesterase PPME1-like [Cucumis melo][more]
XP_038902684.13.91e-22281.89putative pectinesterase 63 [Benincasa hispida][more]
KGN48350.22.16e-21982.21hypothetical protein Csa_003568 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT1G69940.12.1e-10756.05Pectin lyase-like superfamily protein [more]
AT5G07410.16.6e-10655.16Pectin lyase-like superfamily protein [more]
AT5G61680.13.3e-10553.04Pectin lyase-like superfamily protein [more]
AT5G07430.13.3e-9751.54Pectin lyase-like superfamily protein [more]
AT5G07420.13.3e-9750.42Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 71..357
e-value: 7.1E-59
score: 199.0
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 64..365
e-value: 2.4E-94
score: 318.3
NoneNo IPR availablePANTHERPTHR31321ACYL-COA THIOESTER HYDROLASE YBHC-RELATEDcoord: 34..363
NoneNo IPR availablePANTHERPTHR31321:SF87PECTINESTERASE 63-RELATEDcoord: 34..363
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 218..227
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 67..363

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy5G092360.1Chy5G092360.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0045490 pectin catabolic process
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0004672 protein kinase activity