Chy5G092340 (gene) Cucumber (hystrix) v1

Overview
NameChy5G092340
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionPectinesterase
LocationchrH05: 1495610 .. 1498671 (-)
RNA-Seq ExpressionChy5G092340
SyntenyChy5G092340
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACATATTGCATTAATGCTCTTCGAGTTACAAGATGGATAATTTTTCTTGAAGCTTCTGCAGCCATAATCCTCGCCGCATCGCATATGATTCCAACCGAGAAGTCGGAACTAGACGGTTGGTATTCAAAGCATGTGAAAACGTTTAACAAACGTAATAAGACGAAGCTTGATTCGGAGCTGGTGGCTGCAGAAGAAAGCGCCACCGTGATTAAGGTAAGGGGTGATGGAAGTGGAGATTTCAAGACAATTACTGAAGCCATTGAAAGCGTGCCAGCGTGTAACACGAAACGTGTAGTGATTTGGATAGGAGGAGGAGTTTATAAAGAGAAGCTTAAGATTGATAGAAATAAGCCTTTCGTTACTCTGTATGGATCTCCTAATAATATGCCTAACTTGACCTTCAATGGGGATGCTAGCAAATACGGCACCGTTTATAGTGCTACCTTGACGGTTGAAGCTGATTATTTCACTGCAGCTAATCTGATCATTGAGGTATAATTTGTTTATTAAATATGTTTTAAGGTTAAATTAGCAAAATTGTTTGGTAAAGTGTTAGGAATAATAATTTCTACCATAGATATTACTTATGAAATTTTTACAAAGTATAAGGACTATTATTTATCATGTTTTATTATGATATAACTAATATTGACTAGTCAACTTGTAAAGTTTTTGGAATTCTAAGTTTATCCTTTAATGCACATACGTCCATCATTAGTATCAACTATCGATGGTCGCAATTAATAACACGTTTTTCAGATTGAAAGCTAACCCTAATAGCAGTTATATCGGTGATATTCATTGATAGCAACAAAAAACACATTTCTCAAATTAAAAACTATCAGTAATTATCCATGAATATCATTGATAGCAACCGATAATACATTTTTCAAATTGATCAAAGCTACCACTAATAGCAGTTATCAGTTATAGCAATCGATAGTAGTGAGAAATGAAAGTTATCACTAATACCAATTACTTGTTTTTGTGCTAGACATGAGCTTTTTAATATTTTACCAATTCTTATGTTATATATGCAATTACTTTATCCTTTTACGGTATATATATAAATTATTTCATTATGATGAATAGCAAAATGAGAAATTATCAATAACTAAACTTAAATTTAACCCTTTTATAAAAATCATATTTGTTGAAAAACAATACACATAAACAGAATTCATCGCCAAGGCCAGATGGGAAAAGAAAAGGAGAGCAAGCATTAGCAGCAAGAATTTGGGGAAATAAAGTGGCAATTTATAATTGCAAATTCATTGGATTTCAAGATACCCTTTGCGACGACGAGGGTTTACATCTCTACAAAGATTGCTTCATTCAAGGCACTGTTGATTTCATTTTCGGAGGAGGAACTTCCCTTTATTTGGTATAATTTAATTATATTATAATTACTGTTCTTGTATAGATATTTAAATGAATATTATAATTTTAGTTTTGAGAAAATGATTCATTACAGAACACTCAGTTGGATGTGGTGGTCAATGGAGGATTGGGAGTGATAGCAGCCCATTCAAGAGAACAAGAATCTGAGTGCAGTGGATTCTCATTTGTGCATTGTAGCATTACAGGAACTGGCGGAAGAAACACTTATTTGGGAAGAGCTTGGAGGCCAAGATCTAGAGTTGTTTTTGCCTACACTACAATGGCTGATATTATTCACCCCGAAGGCTGGAACGACATGAACAATTTTGGTTACGATAAGTACGTAACAGCTTTCACATTCTTTTTACTTCATTTCTTTTCTAAAGATTAAAATTAGGACAAATATGATTTAAAGTACTTGGGGATTGTTACATTGAATGATACTTTTTCTTTTTCTACTTTTACAGAACGGTTTCGTTCGGAGAATACAAGTGTTGGGGGCCGGGTTCGAACTTGAGCAGGCGTGTGCAATACAGCAAGCAATTGAGTGATGAAGAAGTGAAGCCGTTTGTTAGTCTTGAATACTTGGAGAGTGAGAAGTGGCTACTTCCTCCTCCCAATCACGAAAATTTAGAAAATTAGGATATGTTTTTAATTGACTACGTCATGTGATCCTATGTTATATCATTAACTACTCTCGAGTTCTTAATAATATCGTATCATAAGCTTTCTCTTAATTTCTCATTTGAGAGTCCTTTGCATCATGTCTTCACAGTGATTCTTAAAAACATGTCTTGGTTAATTATGGATTTTTCTTTCTTTTTCGATATTTAAATCTTCCGTTCGCTTCGCTATTAAATAATTATATGTTAATCCCTACTTTCTTCATAAGTCGGATAGTACCTTCATCGTTCTTGTTTCAAATTTGTTTACCAAAACAAACTAAATCTTATGAAGCTAGCTAGCTGGTGAACTTGAGTTGTTGCTAAGGTGTGTGCAAGTAAAATTCACATGAACCTTTTACTTTCTTATAAATAATTTATATAGTACAAACCTATAGAGGCTAAAATCTAAGCACTCTCGTAACATTTTTACACGTTTATTATTTCAAAAGAAAAATCAATTTCAACCTAGAATTTTGAAAACACAATGAGTGACTTAGAGTCACGCTACTAAACCATAGAGAAGGAGAAAAATAAGTGTACAAAATTTTCTTCCTTTTGCATAAGTTAAAAAGAGAGAAGAATTTGATCCAAGAATAGAAACATAGATAATGAGAAGGAGGAACCGATCAGCTATTCACGAAAGTGAAATAATATTTAGGCACAAGAATAAAAAACGAAAAATAGATATTTGCAACGAGAAAGATGAACGAAAAATTATTAACGGAAGAAAAAAAAATATAAGTGACAAGAAATGGCCTTGCATACTCAAAGAATTGTTTTAGAAAATTTGCACCCAACTAAGGCTATTATAAGATAATTAATCAATTGGAACGGTGGGTCAGATTTCATTTTGGGCAAAAACCCGACTTAACTGAGCTTCTGTTCATATCACTCTCAGAATCAAGACTTGAGAGTATCGTGGCCCTCACTCTCCGGCGAGCTGACACCGGATCTTTAATCGTCTTCCCCAGCGCCGGCGATATCAGGATGGAACTGCCACTTTATCACCCATGA

mRNA sequence

ATGACATATTGCATTAATGCTCTTCGAGTTACAAGATGGATAATTTTTCTTGAAGCTTCTGCAGCCATAATCCTCGCCGCATCGCATATGATTCCAACCGAGAAGTCGGAACTAGACGGTTGGTATTCAAAGCATGTGAAAACGTTTAACAAACGTAATAAGACGAAGCTTGATTCGGAGCTGGTGGCTGCAGAAGAAAGCGCCACCGTGATTAAGGTAAGGGGTGATGGAAGTGGAGATTTCAAGACAATTACTGAAGCCATTGAAAGCGTGCCAGCGTGTAACACGAAACGTGTAGTGATTTGGATAGGAGGAGGAGTTTATAAAGAGAAGCTTAAGATTGATAGAAATAAGCCTTTCGTTACTCTGTATGGATCTCCTAATAATATGCCTAACTTGACCTTCAATGGGGATGCTAGCAAATACGGCACCGTTTATAGTGCTACCTTGACGGTTGAAGCTGATTATTTCACTGCAGCTAATCTGATCATTGAGAATTCATCGCCAAGGCCAGATGGGAAAAGAAAAGGAGAGCAAGCATTAGCAGCAAGAATTTGGGGAAATAAAGTGGCAATTTATAATTGCAAATTCATTGGATTTCAAGATACCCTTTGCGACGACGAGGGTTTACATCTCTACAAAGATTGCTTCATTCAAGGCACTAACACTCAGTTGGATGTGGTGGTCAATGGAGGATTGGGAGTGATAGCAGCCCATTCAAGAGAACAAGAATCTGAGTGCAGTGGATTCTCATTTGTGCATTGTAGCATTACAGGAACTGGCGGAAGAAACACTTATTTGGGAAGAGCTTGGAGGCCAAGATCTAGAGTTGTTTTTGCCTACACTACAATGGCTGATATTATTCACCCCGAAGGCTGGAACGACATGAACAATTTTGGTTACGATAAAACGGTTTCGTTCGGAGAATACAAGTGTTGGGGGCCGGGTTCGAACTTGAGCAGGCGTGTGCAATACAGCAAGCAATTGAGTGATGAAGAAGTGAAGCCGTTTGTTAGTCTTGAATACTTGGAGAAATCAAGACTTGAGAGTATCGTGGCCCTCACTCTCCGGCGAGCTGACACCGGATCTTTAATCGTCTTCCCCAGCGCCGGCGATATCAGGATGGAACTGCCACTTTATCACCCATGA

Coding sequence (CDS)

ATGACATATTGCATTAATGCTCTTCGAGTTACAAGATGGATAATTTTTCTTGAAGCTTCTGCAGCCATAATCCTCGCCGCATCGCATATGATTCCAACCGAGAAGTCGGAACTAGACGGTTGGTATTCAAAGCATGTGAAAACGTTTAACAAACGTAATAAGACGAAGCTTGATTCGGAGCTGGTGGCTGCAGAAGAAAGCGCCACCGTGATTAAGGTAAGGGGTGATGGAAGTGGAGATTTCAAGACAATTACTGAAGCCATTGAAAGCGTGCCAGCGTGTAACACGAAACGTGTAGTGATTTGGATAGGAGGAGGAGTTTATAAAGAGAAGCTTAAGATTGATAGAAATAAGCCTTTCGTTACTCTGTATGGATCTCCTAATAATATGCCTAACTTGACCTTCAATGGGGATGCTAGCAAATACGGCACCGTTTATAGTGCTACCTTGACGGTTGAAGCTGATTATTTCACTGCAGCTAATCTGATCATTGAGAATTCATCGCCAAGGCCAGATGGGAAAAGAAAAGGAGAGCAAGCATTAGCAGCAAGAATTTGGGGAAATAAAGTGGCAATTTATAATTGCAAATTCATTGGATTTCAAGATACCCTTTGCGACGACGAGGGTTTACATCTCTACAAAGATTGCTTCATTCAAGGCACTAACACTCAGTTGGATGTGGTGGTCAATGGAGGATTGGGAGTGATAGCAGCCCATTCAAGAGAACAAGAATCTGAGTGCAGTGGATTCTCATTTGTGCATTGTAGCATTACAGGAACTGGCGGAAGAAACACTTATTTGGGAAGAGCTTGGAGGCCAAGATCTAGAGTTGTTTTTGCCTACACTACAATGGCTGATATTATTCACCCCGAAGGCTGGAACGACATGAACAATTTTGGTTACGATAAAACGGTTTCGTTCGGAGAATACAAGTGTTGGGGGCCGGGTTCGAACTTGAGCAGGCGTGTGCAATACAGCAAGCAATTGAGTGATGAAGAAGTGAAGCCGTTTGTTAGTCTTGAATACTTGGAGAAATCAAGACTTGAGAGTATCGTGGCCCTCACTCTCCGGCGAGCTGACACCGGATCTTTAATCGTCTTCCCCAGCGCCGGCGATATCAGGATGGAACTGCCACTTTATCACCCATGA

Protein sequence

MTYCINALRVTRWIIFLEASAAIILAASHMIPTEKSELDGWYSKHVKTFNKRNKTKLDSELVAAEESATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKIDRNKPFVTLYGSPNNMPNLTFNGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKRKGEQALAARIWGNKVAIYNCKFIGFQDTLCDDEGLHLYKDCFIQGTNTQLDVVVNGGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTTMADIIHPEGWNDMNNFGYDKTVSFGEYKCWGPGSNLSRRVQYSKQLSDEEVKPFVSLEYLEKSRLESIVALTLRRADTGSLIVFPSAGDIRMELPLYHP*
Homology
BLAST of Chy5G092340 vs. ExPASy Swiss-Prot
Match: Q9LY19 (Probable pectinesterase 48 OS=Arabidopsis thaliana OX=3702 GN=PME48 PE=2 SV=2)

HSP 1 Score: 335.5 bits (859), Expect = 7.9e-91
Identity = 165/330 (50.00%), Postives = 225/330 (68.18%), Query Frame = 0

Query: 31  IPTEKSELDGWYSKHVKTFNKRNKTKLDSELVAAEESATVIKVRGDGSGDFKTITEAIES 90
           IP  K ++  W++ HV    +R    LD  LVAAE +  +I V   G G+FKT+T+AI+S
Sbjct: 29  IPEGKPQVVQWFNTHVGPLAQRK--GLDPALVAAEAAPRIINVNPKG-GEFKTLTDAIKS 88

Query: 91  VPACNTKRVVIWIGGGVYKEKLKIDRNKPFVTLYGSPNNMPNLTFNGDASKYGTVYSATL 150
           VPA NTKRV+I +  G Y+EK+ IDRNKPF+TL G PN MP +T++G A+KYGTV SA+L
Sbjct: 89  VPAGNTKRVIIKMAHGEYREKVTIDRNKPFITLMGQPNAMPVITYDGTAAKYGTVDSASL 148

Query: 151 TVEADYFTAANLIIENSSPRPDGKRKGEQALAARIWGNKVAIYNCKFIGFQDTLCDDEGL 210
            + +DYF A N++++N++P PDGK KG QAL+ RI GN  A YNCKF GFQDT+CDD G 
Sbjct: 149 IILSDYFMAVNIVVKNTAPAPDGKTKGAQALSMRISGNFAAFYNCKFYGFQDTICDDTGN 208

Query: 211 HLYKDCFIQGT-------------NTQLDVVVNGGLGVIAAHSREQESECSGFSFVHCSI 270
           H +KDC+++GT              TQL  VV  G+ VIAAH+ +   E SG+SFVHC +
Sbjct: 209 HFFKDCYVEGTFDFIFGSGTSMYLGTQLH-VVGDGIRVIAAHAGKSAEEKSGYSFVHCKV 268

Query: 271 TGTGGRNTYLGRAWRPRSRVVFAYTTMADIIHPEGWNDMNNFGYDKTVSFGEYKCWGPGS 330
           TGTGG   YLGRAW    +VV+AYT M  +++P GW +     +DKTV +GEYKC GPGS
Sbjct: 269 TGTGG-GIYLGRAWMSHPKVVYAYTEMTSVVNPTGWQENKTPAHDKTVFYGEYKCSGPGS 328

Query: 331 NLSRRVQYSKQLSDEEVKPFVSLEYLEKSR 348
           + ++RV +++ + D+E   F+SL Y++ S+
Sbjct: 329 HKAKRVPFTQDIDDKEANCFLSLGYIQGSK 353

BLAST of Chy5G092340 vs. ExPASy Swiss-Prot
Match: Q84WM7 (Pectinesterase PPME1 OS=Arabidopsis thaliana OX=3702 GN=PPME1 PE=1 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 1.4e-90
Identity = 165/330 (50.00%), Postives = 225/330 (68.18%), Query Frame = 0

Query: 31  IPTEKSELDGWYSKHVKTFNKRNKTKLDSELVAAEESATVIKVRGDGSGDFKTITEAIES 90
           IP  K ++  W++ +V    +R    LD  LVAAE +  +I V   G G+FKT+T+AI+S
Sbjct: 29  IPEGKPQVAQWFNANVGPLAQRK--GLDPALVAAEAAPRIINVNPKG-GEFKTLTDAIKS 88

Query: 91  VPACNTKRVVIWIGGGVYKEKLKIDRNKPFVTLYGSPNNMPNLTFNGDASKYGTVYSATL 150
           VPA NTKRV+I +  G YKEK+ IDRNKPF+TL G PN MP +T++G A+KYGTV SA+L
Sbjct: 89  VPAGNTKRVIIKMAPGEYKEKVTIDRNKPFITLMGQPNAMPVITYDGTAAKYGTVDSASL 148

Query: 151 TVEADYFTAANLIIENSSPRPDGKRKGEQALAARIWGNKVAIYNCKFIGFQDTLCDDEGL 210
            + +DYF A N++++N++P PDGK KG QAL+ RI GN  A YNCKF GFQDT+CDD G 
Sbjct: 149 IILSDYFMAVNIVVKNTAPAPDGKTKGAQALSMRISGNFAAFYNCKFYGFQDTICDDTGN 208

Query: 211 HLYKDCFIQGT-------------NTQLDVVVNGGLGVIAAHSREQESECSGFSFVHCSI 270
           H +KDC+++GT              TQL  VV  G+ VIAAH+ +   E SG+SFVHC +
Sbjct: 209 HFFKDCYVEGTFDFIFGSGTSMYLGTQLH-VVGDGIRVIAAHAGKSAEEKSGYSFVHCKV 268

Query: 271 TGTGGRNTYLGRAWRPRSRVVFAYTTMADIIHPEGWNDMNNFGYDKTVSFGEYKCWGPGS 330
           TGTGG   YLGRAW    +VV+AYT M  +++P GW +     +DKTV +GEYKC GPGS
Sbjct: 269 TGTGG-GIYLGRAWMSHPKVVYAYTEMTSVVNPTGWQENKTPAHDKTVFYGEYKCSGPGS 328

Query: 331 NLSRRVQYSKQLSDEEVKPFVSLEYLEKSR 348
           + ++RV +++ + D+E   F+SL Y++ S+
Sbjct: 329 HKAKRVPFTQDIDDKEANRFLSLGYIQGSK 353

BLAST of Chy5G092340 vs. ExPASy Swiss-Prot
Match: Q9FKF3 (Putative pectinesterase 63 OS=Arabidopsis thaliana OX=3702 GN=PME63 PE=3 SV=2)

HSP 1 Score: 329.3 bits (843), Expect = 5.7e-89
Identity = 160/287 (55.75%), Postives = 206/287 (71.78%), Query Frame = 0

Query: 73  VRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKIDRNKPFVTLYGSPNNMPN 132
           V+ +G G FKTITEAI SV A NT+RV+I IG GVYKEK+ IDR+KPF+TLYG PN MP 
Sbjct: 45  VKQNGRGHFKTITEAINSVRAGNTRRVIIKIGPGVYKEKVTIDRSKPFITLYGHPNAMPV 104

Query: 133 LTFNGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKRKGEQALAARIWGNKVAI 192
           LTF+G A++YGTV SATL V +DYF A N+I++NS+P PDGKRKG QAL+ RI GNK A 
Sbjct: 105 LTFDGTAAQYGTVDSATLIVLSDYFMAVNIILKNSAPMPDGKRKGAQALSMRISGNKAAF 164

Query: 193 YNCKFIGFQDTLCDDEGLHLYKDCFIQGT-------------NTQLDVVVNGGLGVIAAH 252
           YNCKF G+QDT+CDD G H +KDC+I+GT              TQL+ VV  G+ VI AH
Sbjct: 165 YNCKFYGYQDTICDDTGNHFFKDCYIEGTFDFIFGSGRSLYLGTQLN-VVGDGIRVITAH 224

Query: 253 SREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTTMADIIHPEGWNDMNNF 312
           + +  +E SG+SFVHC +TGT G   YLGR+W    +VV+AYT M+ +++P GW +    
Sbjct: 225 AGKSAAEKSGYSFVHCKVTGT-GTGIYLGRSWMSHPKVVYAYTDMSSVVNPSGWQENREA 284

Query: 313 GYDKTVSFGEYKCWGPGSNLSRRVQYSKQLSDEEVKPFVSLEYLEKS 347
           G DKTV +GEYKC G GS+  +RV+Y++ + D E K F+SL Y++ S
Sbjct: 285 GRDKTVFYGEYKCTGTGSHKEKRVKYTQDIDDIEAKYFISLGYIQGS 329

BLAST of Chy5G092340 vs. ExPASy Swiss-Prot
Match: B2VPR8 (Pectinesterase 2 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 3.6e-83
Identity = 162/331 (48.94%), Postives = 214/331 (64.65%), Query Frame = 0

Query: 31  IPTEKSELDGWYSKHVKTFNKRNKTKLDSELVAAEESATVIKVRGDGSGDFKTITEAIES 90
           IP   ++L+ W+   ++    R K  +D  LV AE  A VIK++ DGSGDFK+I EAI+S
Sbjct: 28  IPANSAQLNSWFDGIIQPVAVR-KATMDPALVTAEGQAKVIKLKSDGSGDFKSINEAIKS 87

Query: 91  VPACNTKRVVIWIGGGVYKEKLKIDRNKPFVTLYG-SPNNMPNLTFNGDASKYGTVYSAT 150
           +P  NTKRV++    G Y EK+KI   K ++T YG  PNNMP L F G A++YGTV SAT
Sbjct: 88  IPDDNTKRVILSFSPGNYSEKVKIGMYKHYITFYGEDPNNMPILVFGGTAAEYGTVDSAT 147

Query: 151 LTVEADYFTAANLIIENSSPRPDGKRKGEQALAARIWGNKVAIYNCKFIGFQDTLCDDEG 210
           L VE++YF+A NL I NS+PRPDGKR G QA A RI G+K + YN K  GFQDTLCDD+G
Sbjct: 148 LIVESNYFSAVNLKIVNSAPRPDGKRVGAQAAALRISGDKASFYNVKIYGFQDTLCDDKG 207

Query: 211 LHLYKDCFIQGT-------------NTQLDVVVNGGLGVIAAHSREQESECSGFSFVHCS 270
            H YKDC+I+GT             NT+L  V      +I A +R+ ESE +G+ FV+C 
Sbjct: 208 KHFYKDCYIEGTVDFIFGSGKSIFLNTELHAVPGDQPAIITAQARKTESEDTGYYFVNCR 267

Query: 271 ITGTGGRNTYLGRAWRPRSRVVFAYTTMADIIHPEGWNDMNNFGYDKTVSFGEYKCWGPG 330
           +TG G    +LGR+W P ++VVFAYT M D IHPEGW  +    ++ TV F EY   GPG
Sbjct: 268 VTGGG---AFLGRSWMPAAKVVFAYTEMGDAIHPEGW-ILVKPEHESTVRFPEYNNKGPG 327

Query: 331 SNLSRRVQYSKQLSDEEVKPFVSLEYLEKSR 348
           +N+ +R ++ K+LSD E K  +SL  +E S+
Sbjct: 328 ANMEKRAKFVKRLSDAEAKQSISLGSIEASK 353

BLAST of Chy5G092340 vs. ExPASy Swiss-Prot
Match: D8VPP5 (Pectinesterase 1 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 6.1e-83
Identity = 160/331 (48.34%), Postives = 215/331 (64.95%), Query Frame = 0

Query: 31  IPTEKSELDGWYSKHVKTFNKRNKTKLDSELVAAEESATVIKVRGDGSGDFKTITEAIES 90
           IP+  ++L+ W+   ++    R K  +D  LV AE    VIK++ DGSGDFK+I EAI+S
Sbjct: 28  IPSNSAQLNSWFDGIIQPVAVR-KATMDPALVTAEGQTKVIKLKSDGSGDFKSINEAIKS 87

Query: 91  VPACNTKRVVIWIGGGVYKEKLKIDRNKPFVTLYG-SPNNMPNLTFNGDASKYGTVYSAT 150
           +P  NTKRV++ +  G Y EK+KI   K ++T YG  PNNMP L F G A++YGTV SAT
Sbjct: 88  IPDDNTKRVILSLAPGNYSEKVKIGMYKHYITFYGEDPNNMPILVFGGTAAEYGTVDSAT 147

Query: 151 LTVEADYFTAANLIIENSSPRPDGKRKGEQALAARIWGNKVAIYNCKFIGFQDTLCDDEG 210
           L VE++YF+A NL I NS+PRPDGKR G QA A RI G+K + YN K  GFQDTLCDD+G
Sbjct: 148 LIVESNYFSAVNLKIVNSAPRPDGKRVGAQAAALRISGDKASFYNVKIYGFQDTLCDDKG 207

Query: 211 LHLYKDCFIQGT-------------NTQLDVVVNGGLGVIAAHSREQESECSGFSFVHCS 270
            H YKDC+I+GT             NT+L  V      +I A +R+ +SE +G+ FV+C 
Sbjct: 208 KHFYKDCYIEGTVDFIFGSGKSIFLNTELHAVPGDQPAIITAQARKTDSEDTGYYFVNCR 267

Query: 271 ITGTGGRNTYLGRAWRPRSRVVFAYTTMADIIHPEGWNDMNNFGYDKTVSFGEYKCWGPG 330
           +TG G    +LGR+W P ++VVFAYT M D IHPEGW  +    ++ TV F EY   GPG
Sbjct: 268 VTGGG---AFLGRSWMPAAKVVFAYTEMVDAIHPEGW-ILVKPEHESTVRFSEYNNKGPG 327

Query: 331 SNLSRRVQYSKQLSDEEVKPFVSLEYLEKSR 348
           +N+ +R ++ K+LSD E K  +SL  +E S+
Sbjct: 328 ANMEKRAKFVKRLSDAEAKQSISLGSIEASK 353

BLAST of Chy5G092340 vs. ExPASy TrEMBL
Match: A0A0A0KEY2 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G428540 PE=4 SV=1)

HSP 1 Score: 658.7 bits (1698), Expect = 1.5e-185
Identity = 322/357 (90.20%), Postives = 335/357 (93.84%), Query Frame = 0

Query: 1   MTYCINALRVTRWIIFLEASAAIILAASHMIPTEKSELDGWYSKHVKTFNKRNKTKLDSE 60
           MTYC NALRVTRWI+FLE SAAIILAASHMIPTEKSELDGWYS+HVK+FNKR+K KLDSE
Sbjct: 1   MTYCNNALRVTRWILFLEVSAAIILAASHMIPTEKSELDGWYSEHVKSFNKRDKMKLDSE 60

Query: 61  LVAAEESATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKIDRNKPF 120
           LVAAEE+ATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKIDRNKPF
Sbjct: 61  LVAAEENATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKIDRNKPF 120

Query: 121 VTLYGSPNNMPNLTFNGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKRKGEQA 180
           VTLYGSPNNMPNLTF+GDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKRKGEQA
Sbjct: 121 VTLYGSPNNMPNLTFDGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKRKGEQA 180

Query: 181 LAARIWGNKVAIYNCKFIGFQDTLCDDEGLHLYKDCFIQGT-------------NTQLDV 240
           LAARIWGNKVAIYNCKFIGFQDTLCDD GLHLYKDCFIQGT             NTQLDV
Sbjct: 181 LAARIWGNKVAIYNCKFIGFQDTLCDDRGLHLYKDCFIQGTVDFIFGGGTSLYLNTQLDV 240

Query: 241 VVNGGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTTMADI 300
           VV+GGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTTMADI
Sbjct: 241 VVDGGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTTMADI 300

Query: 301 IHPEGWNDMNNFGYDKTVSFGEYKCWGPGSNLSRRVQYSKQLSDEEVKPFVSLEYLE 345
           IHP+GWNDM+NFGYDKTVSFGEYKC G GSN S+RVQY KQLSDE+V+ FVSLEYL+
Sbjct: 301 IHPKGWNDMDNFGYDKTVSFGEYKCSGLGSNFSKRVQYGKQLSDEKVQQFVSLEYLK 357

BLAST of Chy5G092340 vs. ExPASy TrEMBL
Match: A0A1S3BMG4 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103491453 PE=4 SV=1)

HSP 1 Score: 602.8 bits (1553), Expect = 9.8e-169
Identity = 298/360 (82.78%), Postives = 318/360 (88.33%), Query Frame = 0

Query: 1   MTYCINALRVTRWIIFLEASAAIILAASHMIPTEKSELDGWYSKHVKTFNKRNKTKLDSE 60
           M YCINALR+TRWI+FLEA AA ILAAS +IPTEKS+LD WY ++VK F  RNK +LD E
Sbjct: 1   MKYCINALRLTRWIMFLEAFAA-ILAASPLIPTEKSQLDEWYWENVKAFGNRNKAELDPE 60

Query: 61  LVAAEESATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKIDRNKPF 120
           LVAAEESATVIKVRGDGSGDFKTI EAIESVP  NTKR+VIWIGGGVYKEKLKIDRNKPF
Sbjct: 61  LVAAEESATVIKVRGDGSGDFKTIAEAIESVPIGNTKRIVIWIGGGVYKEKLKIDRNKPF 120

Query: 121 VTLYGSPNNMPNLTFNGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKRKGEQA 180
           VTLYGSPNNMPNLTF+GDASKYGTVYSATL VEADYFTAANL+IENSSPRPDG RKG QA
Sbjct: 121 VTLYGSPNNMPNLTFDGDASKYGTVYSATLIVEADYFTAANLVIENSSPRPDGVRKGAQA 180

Query: 181 LAARIWGNKVAIYNCKFIGFQDTLCDDEGLHLYKDCFIQGT-------------NTQLDV 240
           LAARI GNK AIYNCKFIGFQDTLCDD+GLHLYKDCFIQGT             NTQLDV
Sbjct: 181 LAARIRGNKAAIYNCKFIGFQDTLCDDDGLHLYKDCFIQGTVDFIFGKGTSLYLNTQLDV 240

Query: 241 VVNGGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTTMADI 300
           V NGGLGVIAAHSR+QES+ SG+SFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTTMADI
Sbjct: 241 VGNGGLGVIAAHSRDQESDGSGYSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTTMADI 300

Query: 301 IHPEGWNDMNNFGYDKTVSFGEYKCWGPGSNLSRRVQYSKQLSDEEVKPFVSLEYLEKSR 348
           IHPEGWNDMN+FGYDKTV FGEYKCWGPGS  S RV+YSKQLS EEV+PFVSL Y++  +
Sbjct: 301 IHPEGWNDMNHFGYDKTVLFGEYKCWGPGSKSSERVKYSKQLSYEEVEPFVSLGYVQSEK 359

BLAST of Chy5G092340 vs. ExPASy TrEMBL
Match: A0A5D3B8A8 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G00490 PE=4 SV=1)

HSP 1 Score: 577.4 bits (1487), Expect = 4.4e-161
Identity = 286/346 (82.66%), Postives = 305/346 (88.15%), Query Frame = 0

Query: 15  IFLEASAAIILAASHMIPTEKSELDGWYSKHVKTFNKRNKTKLDSELVAAEESATVIKVR 74
           +FLEA AA ILAAS +IPTEKS+LD WY ++VK F  RNK +LD ELVAAEESATVIKVR
Sbjct: 1   MFLEAFAA-ILAASPLIPTEKSQLDEWYWENVKAFGNRNKAELDPELVAAEESATVIKVR 60

Query: 75  GDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKIDRNKPFVTLYGSPNNMPNLT 134
           GDGSGDFKTI EAIESVP  NTKR+VIWIGGGVYKEKLKIDRNKPFVTLYGSPNNMPNLT
Sbjct: 61  GDGSGDFKTIAEAIESVPIGNTKRIVIWIGGGVYKEKLKIDRNKPFVTLYGSPNNMPNLT 120

Query: 135 FNGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKRKGEQALAARIWGNKVAIYN 194
           F+GDASKYGTVYSATL VEADYFTAANL+IENSSPRPDG RKG QALAARI GNK AIYN
Sbjct: 121 FDGDASKYGTVYSATLIVEADYFTAANLVIENSSPRPDGVRKGAQALAARIRGNKAAIYN 180

Query: 195 CKFIGFQDTLCDDEGLHLYKDCFIQGT-------------NTQLDVVVNGGLGVIAAHSR 254
           CKFIGFQDTLCDD+GLHLYKDCFIQGT             NTQLDVV NGGLGVIAAHSR
Sbjct: 181 CKFIGFQDTLCDDDGLHLYKDCFIQGTVDFIFGKGTSLYLNTQLDVVGNGGLGVIAAHSR 240

Query: 255 EQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTTMADIIHPEGWNDMNNFGY 314
           +QES+ SG+SFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTTMADIIHPEGWNDMN+FGY
Sbjct: 241 DQESDGSGYSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTTMADIIHPEGWNDMNHFGY 300

Query: 315 DKTVSFGEYKCWGPGSNLSRRVQYSKQLSDEEVKPFVSLEYLEKSR 348
           DKTV FGEYKCWGPGS  S RV+YSKQLS EEV+PFVSL Y++  +
Sbjct: 301 DKTVLFGEYKCWGPGSKSSERVKYSKQLSYEEVEPFVSLGYVQSEK 345

BLAST of Chy5G092340 vs. ExPASy TrEMBL
Match: A0A6J1DHP7 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111020595 PE=4 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 3.2e-143
Identity = 260/360 (72.22%), Postives = 297/360 (82.50%), Query Frame = 0

Query: 1   MTYCINALRVTRWIIFLEASAAIILAASHMIPTEKSELDGWYSKHVKTFNKRNKTKLDSE 60
           M YCI+AL +TR ++ L A+ A+ILAAS  +P +KS+++ W+S +VK    R K +LD  
Sbjct: 1   MKYCIDALSLTR-LLTLLATFALILAASPPVPADKSQVNKWFSDNVKPLADR-KAELDPA 60

Query: 61  LVAAEESATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKIDRNKPF 120
           LVAAEE ATV+KVR DGSGDFKT+TEAI SVP  NTKRVVIWIGGGVYKEKLKI+R KPF
Sbjct: 61  LVAAEEGATVVKVRADGSGDFKTVTEAINSVPTGNTKRVVIWIGGGVYKEKLKIERTKPF 120

Query: 121 VTLYGSPNNMPNLTFNGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKRKGEQA 180
           VTLYGSPNNMPNLTF+GDA+K+GTVYSATLTVE+DYFTAANLII NSSPRPDGKRKG QA
Sbjct: 121 VTLYGSPNNMPNLTFDGDANKFGTVYSATLTVESDYFTAANLIIVNSSPRPDGKRKGAQA 180

Query: 181 LAARIWGNKVAIYNCKFIGFQDTLCDDEGLHLYKDCFIQGT-------------NTQLDV 240
           LAAR+ G K A+YNCKFIGFQDTLCDD+GLHLYKDCFIQGT             N+QLDV
Sbjct: 181 LAARLMGTKAAVYNCKFIGFQDTLCDDDGLHLYKDCFIQGTVDFIFGKGTSLYLNSQLDV 240

Query: 241 VVNGGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTTMADI 300
           V +GGL VI AHSRE+ES+ SG+SFVH SITGTGG+NTYLGRAW  RSR VFAYTTMADI
Sbjct: 241 VGDGGLAVITAHSREEESDPSGYSFVHGSITGTGGKNTYLGRAWMARSRTVFAYTTMADI 300

Query: 301 IHPEGWNDMNNFGYDKTVSFGEYKCWGPGSNLSRRVQYSKQLSDEEVKPFVSLEYLEKSR 348
           I+PEGWNDM + G+D TV FGEYKC GPGS  S RV+YSKQLSD EVKPFVSL +L+  +
Sbjct: 301 INPEGWNDMKHPGFDSTVLFGEYKCSGPGSEPSGRVKYSKQLSDVEVKPFVSLGFLQSDK 358

BLAST of Chy5G092340 vs. ExPASy TrEMBL
Match: A0A0A0KEH2 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G428030 PE=4 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 1.3e-141
Identity = 256/357 (71.71%), Postives = 288/357 (80.67%), Query Frame = 0

Query: 5   INALRVTRWIIFLEASAAIILAASHMIPTEKSELDGWYSKHVKTFNKRNKTKLDSELVAA 64
           INAL +TR + FL A+   ILA S  IP EKS+L GW++++VK F  RNK +LD  L  A
Sbjct: 5   INALAITRLVTFL-ATFTAILAVSPPIPVEKSQLAGWFTENVKPFAVRNKAELDPALATA 64

Query: 65  EESATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKIDRNKPFVTLY 124
           EE+ATVIKV  DG+G+FKT+TEAI SVPA N KRVVIWIG GVYKEKLKIDRNKPFVTLY
Sbjct: 65  EENATVIKVMSDGTGNFKTVTEAIASVPADNKKRVVIWIGVGVYKEKLKIDRNKPFVTLY 124

Query: 125 GS-PNNMPNLTFNGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKRKGEQALAA 184
           GS P NMP LTF+GDA+KYGTVYSATL VEADYFTAANLIIENSSPRPDG RKG QALAA
Sbjct: 125 GSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDGVRKGAQALAA 184

Query: 185 RIWGNKVAIYNCKFIGFQDTLCDDEGLHLYKDCFIQGT-------------NTQLDVVVN 244
           R  G K AIYNCKF+GFQDTLCDD+GLH YKDCFIQGT             NTQLDV  +
Sbjct: 185 RFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYLNTQLDVAGD 244

Query: 245 GGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTTMADIIHP 304
           GGL VI AHSREQE++ SG+SFVHCSITGTGG+NTYLGRAW PRSRVVFAYTT+ADIIHP
Sbjct: 245 GGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFAYTTIADIIHP 304

Query: 305 EGWNDMNNFGYDKTVSFGEYKCWGPGSNLSRRVQYSKQLSDEEVKPFVSLEYLEKSR 348
           EGWNDM + G+DKTV FGEYKC GPG+  + RV Y KQL+D EVKPF+ LEY++  +
Sbjct: 305 EGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEVKPFLGLEYVQSEK 360

BLAST of Chy5G092340 vs. NCBI nr
Match: KGN48073.1 (hypothetical protein Csa_002957 [Cucumis sativus])

HSP 1 Score: 657 bits (1696), Expect = 1.81e-236
Identity = 322/357 (90.20%), Postives = 335/357 (93.84%), Query Frame = 0

Query: 1   MTYCINALRVTRWIIFLEASAAIILAASHMIPTEKSELDGWYSKHVKTFNKRNKTKLDSE 60
           MTYC NALRVTRWI+FLE SAAIILAASHMIPTEKSELDGWYS+HVK+FNKR+K KLDSE
Sbjct: 1   MTYCNNALRVTRWILFLEVSAAIILAASHMIPTEKSELDGWYSEHVKSFNKRDKMKLDSE 60

Query: 61  LVAAEESATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKIDRNKPF 120
           LVAAEE+ATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKIDRNKPF
Sbjct: 61  LVAAEENATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKIDRNKPF 120

Query: 121 VTLYGSPNNMPNLTFNGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKRKGEQA 180
           VTLYGSPNNMPNLTF+GDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKRKGEQA
Sbjct: 121 VTLYGSPNNMPNLTFDGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKRKGEQA 180

Query: 181 LAARIWGNKVAIYNCKFIGFQDTLCDDEGLHLYKDCFIQGT-------------NTQLDV 240
           LAARIWGNKVAIYNCKFIGFQDTLCDD GLHLYKDCFIQGT             NTQLDV
Sbjct: 181 LAARIWGNKVAIYNCKFIGFQDTLCDDRGLHLYKDCFIQGTVDFIFGGGTSLYLNTQLDV 240

Query: 241 VVNGGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTTMADI 300
           VV+GGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTTMADI
Sbjct: 241 VVDGGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTTMADI 300

Query: 301 IHPEGWNDMNNFGYDKTVSFGEYKCWGPGSNLSRRVQYSKQLSDEEVKPFVSLEYLE 344
           IHP+GWNDM+NFGYDKTVSFGEYKC G GSN S+RVQY KQLSDE+V+ FVSLEYL+
Sbjct: 301 IHPKGWNDMDNFGYDKTVSFGEYKCSGLGSNFSKRVQYGKQLSDEKVQQFVSLEYLK 357

BLAST of Chy5G092340 vs. NCBI nr
Match: XP_008449623.1 (PREDICTED: putative pectinesterase 63 [Cucumis melo])

HSP 1 Score: 602 bits (1551), Expect = 1.89e-214
Identity = 298/360 (82.78%), Postives = 318/360 (88.33%), Query Frame = 0

Query: 1   MTYCINALRVTRWIIFLEASAAIILAASHMIPTEKSELDGWYSKHVKTFNKRNKTKLDSE 60
           M YCINALR+TRWI+FLEA AAI LAAS +IPTEKS+LD WY ++VK F  RNK +LD E
Sbjct: 1   MKYCINALRLTRWIMFLEAFAAI-LAASPLIPTEKSQLDEWYWENVKAFGNRNKAELDPE 60

Query: 61  LVAAEESATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKIDRNKPF 120
           LVAAEESATVIKVRGDGSGDFKTI EAIESVP  NTKR+VIWIGGGVYKEKLKIDRNKPF
Sbjct: 61  LVAAEESATVIKVRGDGSGDFKTIAEAIESVPIGNTKRIVIWIGGGVYKEKLKIDRNKPF 120

Query: 121 VTLYGSPNNMPNLTFNGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKRKGEQA 180
           VTLYGSPNNMPNLTF+GDASKYGTVYSATL VEADYFTAANL+IENSSPRPDG RKG QA
Sbjct: 121 VTLYGSPNNMPNLTFDGDASKYGTVYSATLIVEADYFTAANLVIENSSPRPDGVRKGAQA 180

Query: 181 LAARIWGNKVAIYNCKFIGFQDTLCDDEGLHLYKDCFIQGT-------------NTQLDV 240
           LAARI GNK AIYNCKFIGFQDTLCDD+GLHLYKDCFIQGT             NTQLDV
Sbjct: 181 LAARIRGNKAAIYNCKFIGFQDTLCDDDGLHLYKDCFIQGTVDFIFGKGTSLYLNTQLDV 240

Query: 241 VVNGGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTTMADI 300
           V NGGLGVIAAHSR+QES+ SG+SFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTTMADI
Sbjct: 241 VGNGGLGVIAAHSRDQESDGSGYSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTTMADI 300

Query: 301 IHPEGWNDMNNFGYDKTVSFGEYKCWGPGSNLSRRVQYSKQLSDEEVKPFVSLEYLEKSR 347
           IHPEGWNDMN+FGYDKTV FGEYKCWGPGS  S RV+YSKQLS EEV+PFVSL Y++  +
Sbjct: 301 IHPEGWNDMNHFGYDKTVLFGEYKCWGPGSKSSERVKYSKQLSYEEVEPFVSLGYVQSEK 359

BLAST of Chy5G092340 vs. NCBI nr
Match: KAA0061750.1 (putative pectinesterase 63 [Cucumis melo var. makuwa] >TYJ96090.1 putative pectinesterase 63 [Cucumis melo var. makuwa])

HSP 1 Score: 575 bits (1483), Expect = 2.55e-204
Identity = 286/345 (82.90%), Postives = 304/345 (88.12%), Query Frame = 0

Query: 16  FLEASAAIILAASHMIPTEKSELDGWYSKHVKTFNKRNKTKLDSELVAAEESATVIKVRG 75
           FLEA AAI LAAS +IPTEKS+LD WY ++VK F  RNK +LD ELVAAEESATVIKVRG
Sbjct: 2   FLEAFAAI-LAASPLIPTEKSQLDEWYWENVKAFGNRNKAELDPELVAAEESATVIKVRG 61

Query: 76  DGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKIDRNKPFVTLYGSPNNMPNLTF 135
           DGSGDFKTI EAIESVP  NTKR+VIWIGGGVYKEKLKIDRNKPFVTLYGSPNNMPNLTF
Sbjct: 62  DGSGDFKTIAEAIESVPIGNTKRIVIWIGGGVYKEKLKIDRNKPFVTLYGSPNNMPNLTF 121

Query: 136 NGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKRKGEQALAARIWGNKVAIYNC 195
           +GDASKYGTVYSATL VEADYFTAANL+IENSSPRPDG RKG QALAARI GNK AIYNC
Sbjct: 122 DGDASKYGTVYSATLIVEADYFTAANLVIENSSPRPDGVRKGAQALAARIRGNKAAIYNC 181

Query: 196 KFIGFQDTLCDDEGLHLYKDCFIQGT-------------NTQLDVVVNGGLGVIAAHSRE 255
           KFIGFQDTLCDD+GLHLYKDCFIQGT             NTQLDVV NGGLGVIAAHSR+
Sbjct: 182 KFIGFQDTLCDDDGLHLYKDCFIQGTVDFIFGKGTSLYLNTQLDVVGNGGLGVIAAHSRD 241

Query: 256 QESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTTMADIIHPEGWNDMNNFGYD 315
           QES+ SG+SFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTTMADIIHPEGWNDMN+FGYD
Sbjct: 242 QESDGSGYSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTTMADIIHPEGWNDMNHFGYD 301

Query: 316 KTVSFGEYKCWGPGSNLSRRVQYSKQLSDEEVKPFVSLEYLEKSR 347
           KTV FGEYKCWGPGS  S RV+YSKQLS EEV+PFVSL Y++  +
Sbjct: 302 KTVLFGEYKCWGPGSKSSERVKYSKQLSYEEVEPFVSLGYVQSEK 345

BLAST of Chy5G092340 vs. NCBI nr
Match: XP_004140274.3 (putative pectinesterase 63 [Cucumis sativus])

HSP 1 Score: 560 bits (1444), Expect = 5.87e-199
Identity = 274/302 (90.73%), Postives = 283/302 (93.71%), Query Frame = 0

Query: 56  KLDSELVAAEESATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKID 115
           KLDSELVAAEE+ATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKID
Sbjct: 2   KLDSELVAAEENATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKID 61

Query: 116 RNKPFVTLYGSPNNMPNLTFNGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKR 175
           RNKPFVTLYGSPNNMPNLTF+GDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKR
Sbjct: 62  RNKPFVTLYGSPNNMPNLTFDGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKR 121

Query: 176 KGEQALAARIWGNKVAIYNCKFIGFQDTLCDDEGLHLYKDCFIQGT-------------N 235
           KGEQALAARIWGNKVAIYNCKFIGFQDTLCDD GLHLYKDCFIQGT             N
Sbjct: 122 KGEQALAARIWGNKVAIYNCKFIGFQDTLCDDRGLHLYKDCFIQGTVDFIFGGGTSLYLN 181

Query: 236 TQLDVVVNGGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYT 295
           TQLDVVV+GGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYT
Sbjct: 182 TQLDVVVDGGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYT 241

Query: 296 TMADIIHPEGWNDMNNFGYDKTVSFGEYKCWGPGSNLSRRVQYSKQLSDEEVKPFVSLEY 344
           TMADIIHP+GWNDM+NFGYDKTVSFGEYKC G GSN S+RVQY KQLSDE+V+ FVSLEY
Sbjct: 242 TMADIIHPKGWNDMDNFGYDKTVSFGEYKCSGLGSNFSKRVQYGKQLSDEKVQQFVSLEY 301

BLAST of Chy5G092340 vs. NCBI nr
Match: XP_038902684.1 (putative pectinesterase 63 [Benincasa hispida])

HSP 1 Score: 537 bits (1383), Expect = 6.77e-189
Identity = 268/360 (74.44%), Postives = 300/360 (83.33%), Query Frame = 0

Query: 1   MTYCINALRVTRWIIFLEASAAIILAASHMIPTEKSELDGWYSKHVKTFNKRNKTKLDSE 60
           M YCI+AL +TR +IFL A AAI  A S  +P EKS+L+GW+S++VK F    K +LD  
Sbjct: 1   MKYCIDALGLTRLVIFLAAFAAIF-AVSPPVPAEKSQLEGWFSENVKPFADC-KAELDPA 60

Query: 61  LVAAEESATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKIDRNKPF 120
           LVAAEE+ATV+KVR DGSGDFKT+TEAI SVPA NT+RVVIWIG GVYKEKLKIDRNKPF
Sbjct: 61  LVAAEENATVVKVRADGSGDFKTVTEAINSVPAGNTRRVVIWIGEGVYKEKLKIDRNKPF 120

Query: 121 VTLYGSPNNMPNLTFNGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKRKGEQA 180
           VTLYGSPNNMPNLTF+GDA+KYGTVYSATL VE+DYF AANL+IENSSPRPDG RKG QA
Sbjct: 121 VTLYGSPNNMPNLTFDGDAAKYGTVYSATLIVESDYFVAANLVIENSSPRPDGIRKGAQA 180

Query: 181 LAARIWGNKVAIYNCKFIGFQDTLCDDEGLHLYKDCFIQGT-------------NTQLDV 240
           LAAR+ G K AIYNCKFIGFQDTLCDDEGLHLYKDCFIQGT             N++LDV
Sbjct: 181 LAARLMGTKAAIYNCKFIGFQDTLCDDEGLHLYKDCFIQGTVDFIFGKGTSLYLNSRLDV 240

Query: 241 VVNGGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTTMADI 300
           V +GGL VI AHSRE+ES+ SG+SF HCSITGTGG+NTYLGRAW PRSRVVFAYTTMADI
Sbjct: 241 VGDGGLAVITAHSREEESDSSGYSFAHCSITGTGGKNTYLGRAWMPRSRVVFAYTTMADI 300

Query: 301 IHPEGWNDMNNFGYDKTVSFGEYKCWGPGSNLSRRVQYSKQLSDEEVKPFVSLEYLEKSR 347
           IH EGWNDMN+ GYD TV FGEYKC GPG+ LS RV+YSKQLSD EVKPF+SL YL+  +
Sbjct: 301 IHAEGWNDMNHSGYDATVLFGEYKCSGPGAELSGRVKYSKQLSDVEVKPFISLGYLQSEK 358

BLAST of Chy5G092340 vs. TAIR 10
Match: AT5G07410.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 335.5 bits (859), Expect = 5.6e-92
Identity = 165/330 (50.00%), Postives = 225/330 (68.18%), Query Frame = 0

Query: 31  IPTEKSELDGWYSKHVKTFNKRNKTKLDSELVAAEESATVIKVRGDGSGDFKTITEAIES 90
           IP  K ++  W++ HV    +R    LD  LVAAE +  +I V   G G+FKT+T+AI+S
Sbjct: 29  IPEGKPQVVQWFNTHVGPLAQRK--GLDPALVAAEAAPRIINVNPKG-GEFKTLTDAIKS 88

Query: 91  VPACNTKRVVIWIGGGVYKEKLKIDRNKPFVTLYGSPNNMPNLTFNGDASKYGTVYSATL 150
           VPA NTKRV+I +  G Y+EK+ IDRNKPF+TL G PN MP +T++G A+KYGTV SA+L
Sbjct: 89  VPAGNTKRVIIKMAHGEYREKVTIDRNKPFITLMGQPNAMPVITYDGTAAKYGTVDSASL 148

Query: 151 TVEADYFTAANLIIENSSPRPDGKRKGEQALAARIWGNKVAIYNCKFIGFQDTLCDDEGL 210
            + +DYF A N++++N++P PDGK KG QAL+ RI GN  A YNCKF GFQDT+CDD G 
Sbjct: 149 IILSDYFMAVNIVVKNTAPAPDGKTKGAQALSMRISGNFAAFYNCKFYGFQDTICDDTGN 208

Query: 211 HLYKDCFIQGT-------------NTQLDVVVNGGLGVIAAHSREQESECSGFSFVHCSI 270
           H +KDC+++GT              TQL  VV  G+ VIAAH+ +   E SG+SFVHC +
Sbjct: 209 HFFKDCYVEGTFDFIFGSGTSMYLGTQLH-VVGDGIRVIAAHAGKSAEEKSGYSFVHCKV 268

Query: 271 TGTGGRNTYLGRAWRPRSRVVFAYTTMADIIHPEGWNDMNNFGYDKTVSFGEYKCWGPGS 330
           TGTGG   YLGRAW    +VV+AYT M  +++P GW +     +DKTV +GEYKC GPGS
Sbjct: 269 TGTGG-GIYLGRAWMSHPKVVYAYTEMTSVVNPTGWQENKTPAHDKTVFYGEYKCSGPGS 328

Query: 331 NLSRRVQYSKQLSDEEVKPFVSLEYLEKSR 348
           + ++RV +++ + D+E   F+SL Y++ S+
Sbjct: 329 HKAKRVPFTQDIDDKEANCFLSLGYIQGSK 353

BLAST of Chy5G092340 vs. TAIR 10
Match: AT1G69940.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 334.7 bits (857), Expect = 9.6e-92
Identity = 165/330 (50.00%), Postives = 225/330 (68.18%), Query Frame = 0

Query: 31  IPTEKSELDGWYSKHVKTFNKRNKTKLDSELVAAEESATVIKVRGDGSGDFKTITEAIES 90
           IP  K ++  W++ +V    +R    LD  LVAAE +  +I V   G G+FKT+T+AI+S
Sbjct: 29  IPEGKPQVAQWFNANVGPLAQRK--GLDPALVAAEAAPRIINVNPKG-GEFKTLTDAIKS 88

Query: 91  VPACNTKRVVIWIGGGVYKEKLKIDRNKPFVTLYGSPNNMPNLTFNGDASKYGTVYSATL 150
           VPA NTKRV+I +  G YKEK+ IDRNKPF+TL G PN MP +T++G A+KYGTV SA+L
Sbjct: 89  VPAGNTKRVIIKMAPGEYKEKVTIDRNKPFITLMGQPNAMPVITYDGTAAKYGTVDSASL 148

Query: 151 TVEADYFTAANLIIENSSPRPDGKRKGEQALAARIWGNKVAIYNCKFIGFQDTLCDDEGL 210
            + +DYF A N++++N++P PDGK KG QAL+ RI GN  A YNCKF GFQDT+CDD G 
Sbjct: 149 IILSDYFMAVNIVVKNTAPAPDGKTKGAQALSMRISGNFAAFYNCKFYGFQDTICDDTGN 208

Query: 211 HLYKDCFIQGT-------------NTQLDVVVNGGLGVIAAHSREQESECSGFSFVHCSI 270
           H +KDC+++GT              TQL  VV  G+ VIAAH+ +   E SG+SFVHC +
Sbjct: 209 HFFKDCYVEGTFDFIFGSGTSMYLGTQLH-VVGDGIRVIAAHAGKSAEEKSGYSFVHCKV 268

Query: 271 TGTGGRNTYLGRAWRPRSRVVFAYTTMADIIHPEGWNDMNNFGYDKTVSFGEYKCWGPGS 330
           TGTGG   YLGRAW    +VV+AYT M  +++P GW +     +DKTV +GEYKC GPGS
Sbjct: 269 TGTGG-GIYLGRAWMSHPKVVYAYTEMTSVVNPTGWQENKTPAHDKTVFYGEYKCSGPGS 328

Query: 331 NLSRRVQYSKQLSDEEVKPFVSLEYLEKSR 348
           + ++RV +++ + D+E   F+SL Y++ S+
Sbjct: 329 HKAKRVPFTQDIDDKEANRFLSLGYIQGSK 353

BLAST of Chy5G092340 vs. TAIR 10
Match: AT5G61680.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 329.3 bits (843), Expect = 4.0e-90
Identity = 160/287 (55.75%), Postives = 206/287 (71.78%), Query Frame = 0

Query: 73  VRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKIDRNKPFVTLYGSPNNMPN 132
           V+ +G G FKTITEAI SV A NT+RV+I IG GVYKEK+ IDR+KPF+TLYG PN MP 
Sbjct: 45  VKQNGRGHFKTITEAINSVRAGNTRRVIIKIGPGVYKEKVTIDRSKPFITLYGHPNAMPV 104

Query: 133 LTFNGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKRKGEQALAARIWGNKVAI 192
           LTF+G A++YGTV SATL V +DYF A N+I++NS+P PDGKRKG QAL+ RI GNK A 
Sbjct: 105 LTFDGTAAQYGTVDSATLIVLSDYFMAVNIILKNSAPMPDGKRKGAQALSMRISGNKAAF 164

Query: 193 YNCKFIGFQDTLCDDEGLHLYKDCFIQGT-------------NTQLDVVVNGGLGVIAAH 252
           YNCKF G+QDT+CDD G H +KDC+I+GT              TQL+ VV  G+ VI AH
Sbjct: 165 YNCKFYGYQDTICDDTGNHFFKDCYIEGTFDFIFGSGRSLYLGTQLN-VVGDGIRVITAH 224

Query: 253 SREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTTMADIIHPEGWNDMNNF 312
           + +  +E SG+SFVHC +TGT G   YLGR+W    +VV+AYT M+ +++P GW +    
Sbjct: 225 AGKSAAEKSGYSFVHCKVTGT-GTGIYLGRSWMSHPKVVYAYTDMSSVVNPSGWQENREA 284

Query: 313 GYDKTVSFGEYKCWGPGSNLSRRVQYSKQLSDEEVKPFVSLEYLEKS 347
           G DKTV +GEYKC G GS+  +RV+Y++ + D E K F+SL Y++ S
Sbjct: 285 GRDKTVFYGEYKCTGTGSHKEKRVKYTQDIDDIEAKYFISLGYIQGS 329

BLAST of Chy5G092340 vs. TAIR 10
Match: AT5G07430.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 308.9 bits (790), Expect = 5.6e-84
Identity = 156/341 (45.75%), Postives = 221/341 (64.81%), Query Frame = 0

Query: 19  ASAAIILAASHMIPTEKSELDGWYSKHVKTFNKRNKTKLDSELVAAEESATVIKVRGDGS 78
           AS  ++   +  IP  ++++  W+  +VK +++R  T LD  L AAE +  +I V   G 
Sbjct: 16  ASPVVLATDTDPIPENRAQIPQWFKTNVKPYSQRKGT-LDPALEAAEAARQIITVNQKGG 75

Query: 79  GDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKIDRNKPFVTLYGSPNNMPNLTFNGD 138
            +FKT+ EAI+S+P  N  RV+I +  GVY EK+ ID  +PF+TL G P     LT++G 
Sbjct: 76  ANFKTLNEAIKSIPTGNKNRVIIKLAPGVYNEKVTIDIARPFITLLGQPGAETVLTYHGT 135

Query: 139 ASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKRKGEQALAARIWGNKVAIYNCKFI 198
           A++YGTV SATL V A+YF AA+L I+N++P P    +G QALA RI  +K A Y+C+F 
Sbjct: 136 AAQYGTVESATLIVWAEYFQAAHLTIKNTAPMPKPGSQG-QALAMRINADKAAFYSCRFH 195

Query: 199 GFQDTLCDDEGLHLYKDCFIQGT-------------NTQLDVVVNGGLGVIAAHSREQES 258
           GFQDTLCDD+G H +KDC+I+GT             NTQL   V  GL VI A  R+  +
Sbjct: 196 GFQDTLCDDKGNHFFKDCYIEGTYDFIFGRGASLYLNTQLH-AVGDGLRVITAQGRQSAT 255

Query: 259 ECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTTMADIIHPEGWNDMNNFGYDKTV 318
           E +G++FVHC +TGT G   YLGR+W    +VV+A+T M  +++P GW +  N GYDKTV
Sbjct: 256 EQNGYTFVHCKVTGT-GTGIYLGRSWMSHPKVVYAFTEMTSVVNPSGWRENLNRGYDKTV 315

Query: 319 SFGEYKCWGPGSNLSRRVQYSKQLSDEEVKPFVSLEYLEKS 347
            +GEYKC+GPGS+L +RV Y++ +   EV PF++L Y++ S
Sbjct: 316 FYGEYKCFGPGSHLEKRVPYTQDIDKNEVTPFLTLGYIKGS 352

BLAST of Chy5G092340 vs. TAIR 10
Match: AT5G07420.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 303.9 bits (777), Expect = 1.8e-82
Identity = 160/354 (45.20%), Postives = 225/354 (63.56%), Query Frame = 0

Query: 7   ALRVTRWIIFLEASAAIILAASHMIPTEKSELDGWYSKHVKTFNKRNKTKLDSELVAAEE 66
           +L +   ++F  AS  ++      IP +++++  W+  +VK F++R  T LD EL AAE 
Sbjct: 5   SLALVALLVFF-ASPVVLADDITPIPADRAQIPQWFMANVKPFSQRRGT-LDPELEAAEA 64

Query: 67  SATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKIDRNKPFVTLYGS 126
           S  VI V  +G GDFKTI  AI+S+P  N  RV+I +  G+Y EK+ +D  +P+VTL G 
Sbjct: 65  SRRVIIVNQNGGGDFKTINAAIKSIPLANKNRVIIKLAPGIYHEKVTVDVGRPYVTLLGK 124

Query: 127 PNNMPNLTFNGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGKRKGEQALAARIW 186
           P    NLT+ G A+KYGTV SATL V A  F AANL I N+SP P    +G QALA RI 
Sbjct: 125 PGAETNLTYAGTAAKYGTVESATLIVWATNFLAANLNIINTSPMPKPGTQG-QALAMRIN 184

Query: 187 GNKVAIYNCKFIGFQDTLCDDEGLHLYKDCFIQGT-------------NTQLDVVVNGGL 246
           G+K A YNC+F GFQDTLCDD G H +K+C+I+GT              TQL   V  GL
Sbjct: 185 GDKAAFYNCRFYGFQDTLCDDRGNHFFKNCYIEGTYDFIFGRGASLYLTTQLH-AVGDGL 244

Query: 247 GVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAYTTMADIIHPEGW 306
            VIAAH+R+  +E +G+SFVHC +TG  G   YLGRAW    +VV++YT M+ +++P GW
Sbjct: 245 RVIAAHNRQSTTEQNGYSFVHCKVTGV-GTGIYLGRAWMSHPKVVYSYTEMSSVVNPSGW 304

Query: 307 NDMNNFGYDKTVSFGEYKCWGPGSNLSRRVQYSKQLSDEEVKPFVSLEYLEKSR 348
            +     +DKTV +GEY C GPGS+ ++RV +++ + ++E   F++L Y++ S+
Sbjct: 305 QENRVRAHDKTVFYGEYMCTGPGSHKAKRVAHTQDIDNKEASQFLTLGYIKGSK 353

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LY197.9e-9150.00Probable pectinesterase 48 OS=Arabidopsis thaliana OX=3702 GN=PME48 PE=2 SV=2[more]
Q84WM71.4e-9050.00Pectinesterase PPME1 OS=Arabidopsis thaliana OX=3702 GN=PPME1 PE=1 SV=1[more]
Q9FKF35.7e-8955.75Putative pectinesterase 63 OS=Arabidopsis thaliana OX=3702 GN=PME63 PE=3 SV=2[more]
B2VPR83.6e-8348.94Pectinesterase 2 OS=Olea europaea OX=4146 PE=1 SV=1[more]
D8VPP56.1e-8348.34Pectinesterase 1 OS=Olea europaea OX=4146 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KEY21.5e-18590.20Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G428540 PE=4 SV=1[more]
A0A1S3BMG49.8e-16982.78Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103491453 PE=4 SV=1[more]
A0A5D3B8A84.4e-16182.66Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G00490... [more]
A0A6J1DHP73.2e-14372.22Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111020595 PE=4 SV=1[more]
A0A0A0KEH21.3e-14171.71Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G428030 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
KGN48073.11.81e-23690.20hypothetical protein Csa_002957 [Cucumis sativus][more]
XP_008449623.11.89e-21482.78PREDICTED: putative pectinesterase 63 [Cucumis melo][more]
KAA0061750.12.55e-20482.90putative pectinesterase 63 [Cucumis melo var. makuwa] >TYJ96090.1 putative pecti... [more]
XP_004140274.35.87e-19990.73putative pectinesterase 63 [Cucumis sativus][more]
XP_038902684.16.77e-18974.44putative pectinesterase 63 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G07410.15.6e-9250.00Pectin lyase-like superfamily protein [more]
AT1G69940.19.6e-9250.00Pectin lyase-like superfamily protein [more]
AT5G61680.14.0e-9055.75Pectin lyase-like superfamily protein [more]
AT5G07430.15.6e-8445.75Pectin lyase-like superfamily protein [more]
AT5G07420.11.8e-8245.20Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 72..344
e-value: 4.3E-47
score: 160.3
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 64..353
e-value: 1.5E-84
score: 286.1
NoneNo IPR availablePANTHERPTHR31321:SF87PECTINESTERASE 63-RELATEDcoord: 34..346
NoneNo IPR availablePANTHERPTHR31321ACYL-COA THIOESTER HYDROLASE YBHC-RELATEDcoord: 34..346
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 67..345

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy5G092340.1Chy5G092340.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity