Chy5G092220 (gene) Cucumber (hystrix) v1

Overview
NameChy5G092220
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionpre-rRNA-processing protein las1
LocationchrH05: 1381983 .. 1386840 (+)
RNA-Seq ExpressionChy5G092220
SyntenyChy5G092220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTCGCTGTTAGGATTTGAAGAGGTGCCGGGAGAGAATGAAGAAGAGAATTTGTCGACGTACAGCCGCAAATTGGTACCCTGGTCAACTTGGAACGAATGGCTTTTCGTTAGTCAGTCACTCTTCTCTGACTCTCCAGACTCCGTCGCCGCCGCTCTGAGCAGAGTAAGCTTACTTATCTCCCCCGCATGTTTATGCTCCATTGTGCTAACTTTCGAAGTTGCATTTGGGACCTGTAACTATAAATTTCACTATGATGAACTGTTGTGATAAACTTTGAGCACCTTGGACTCTCCACAATGCCATGTACCATAGTTTCCTTGCCTCAGTACTTTCCATCCAATTCTATTTTTGTTGTCTGGATTCAAATTGCGTATTTTCTGTTTTTTTTTTTTTTGCTTATGTTTTGTGATTTTGAATTCAGATATCAACATGGAGAAGCAGAGGATGTCTTCCAGTTGTCATTGAAGTTACAGCGTCGATTATCGAAATCCAACAAAAGGATCCTTACTTCATGTAAGCTTCAAGTTCTAGTTCTTACTATCTTATTTCATGGTAATATTCACTGTATTTTCCTGCTCGAGCAATTTTTTTCCCTAGTTCTTCCATGCCATGTTAAAAAAAGAAAAGAATGAAAGTAGAAGCATGCATTCTGAGATTAGAAATTACGAGAATTTGAACGTCAAAAGATCTATAATCTATCAAATGGTTTGATATCTTAAAGATCGCAAACAATCAAACTGCGCATTGACTATTTCCTTCTATTGCATATCTCACTAATATTAATGCTGCTATGTGTTTGAGTACAAATATTCTTTAATATTATATAGTGTGTTTTTTATGTTCAATATTTTCGTGTGCAAGAAAAACTCTTTGTGTTCAATATTTTCATTTATTCATTCATTGCAGAAAGAACCAATCAATGGATGCTTCAGTTCGTGGTACAGAGAACCAATTAAATCTTGTTTCACTTTCAGAGGAAGCACTAGCCATGTTATATTGCATGGCCATTATGAGGTATATTTTCTTTTTCTTTTATAGTAAAGTACCATGCAAAGCTTTCTTAACTTGTTACTTGTTATTCTAACCCTCTAAGTATGAATATTAAGAGTGGTTATCATATGGTGAAACTACCTTTCTTTTTTTGGTATTACTTATCCACGAAATAGTCTTGCCATTGGTTTTGAAAGAAATACTGAAGTATATTTATTGGTGTAAAGTTCCTATTTCCTAGAATGTCAATGGCCATGTTCATTTATATTACTTAGTTTCTGGGATGCACTTACTATATTATGGCCTTGAACTGATTACATGAGCTCAGGGTTGCCATATAGAAAAAAGGTTAATTAGAATATTGGAACATGCCTTCCACGTTAACCTATTTGCATTAACTGTACAAGTTTGAGGTAAGATGTTTTATAGCCTGGAAATATTCATTCCACACGCATCATTATTTTACTGATTAAAGATCAGATAGAGCCAGCTAGCTGTTATGTGGACCTTTATATTGATTTTAAATAAGATTGTGGAACAGAAGTTAACTTCTTCCGAAGAATTACAAAGTCCTCACTATATTCTTGATTTGAAGGCTTGTAAATGGTGTTGTCGAGAAGACACGTAAGAAAACAGAAGTTTCAATTGCCGTGGCAGCTGATGCTATTGGTTTACCCCGTTTACTGATTGATATTCGTCATGGTAAGTATCATCAAACATCATTTTGTATGCTATCATTTTCTTTTTTCTAGTTTTATGAACCTTTTTTGTAGTCTGCAAGTTTTATCACCCCGAAGTATATATTGCATTGAGATTATTTTTCTTTCTTTCTTTTAATATTGTTGGAGCTTACCATAACTGTGTGACGGTTTATTGTACATTTGTCCTCACTGCTGGGTTATCTTACATTGACTGATTGTAATTATTTTCACGTTTCTCTTGGCATTGGTTTTAGAGGGTTCTCATCGTGAACTTCCAGCTCTTCAGGTGGTTCGACATGCCTCAGCCAAGGTATGACTATGAAGTATGAACTCTCAACATTTGATAGTTTCCATCTTCATTAGCGCCTTTTCTTTATAGTGATTTGTTTCTGTTTAAGATTGTTTCATTTGACATCATTTGAAAAGTGGAATACGAAATCATTCGCGAATTTTCATCACTTGTCTATTCTATCCTTTACATATTTTGTTTACATCATTTTGTACTTGAACATGTCACTTTGCCGTAGAAGTATTTTAAATATTATTAGATTAACTGAGTTGGGAGGTAAACTGAAACCTTCTTGAGTTTGGTGATTTCAGGCGCTTTGTTGGTTGAAATCTTACTATTGGGAACCTCAGGAGAAAGCTATTCCGTTTCAAGGTGATATAAGTACTTCCATTAGAAAGGAAATCAAATCCAAACTTCGTGGTTTGGCTTTCAATTTAGAAATGAAGCAAAATCCTCAGCTGGAATCTGCCCTGGTCAAGCCAAAGCGTATGTATTTAATTTCTCCCTTTCTCATGACCACATTTTATATATCCTTTAAACTCTTTTGTTATTTTGTGAAGCAGTCTTGTAAATGGTCTTGTTTCTATCTTTAACTAATTTGTGGAGGCATTGATTTTATCTTAAACGGAAGATCTTGAGATGAGACAGGTCATTCAAAGAAAGCTAAATTAGTATTATGCATCCAACATAATAGACAGGATCGAGAGATTTTAGAACACGGACTTTTTTAGTCCTGCTTTCTAACATAGTCTTTTGGTTTTTAATTTTGTTAGAATAAGGTTATTTGCTAGGCTTTTTTTTCCTTGTTGAAATGAATTAGGAGTTTTCACTGAATAAGTGCAAATTAAAAGTTTAATCAAACAGTGTTTTAATAATCAAACCAAGAATCCTCACCTTACCTTCTGTGGGTAGCTCTCTTGCTTTCTTGAGAACACTTCGATTCCTTTCTATCTATAGACCCAAACAAAGCTTTTGACCATGTTGACCCATAACACTCTTGCTTTCCTTTTCTCAATTTGTCACATCAAGGACGCCGATTGTATTTATTCTTTTTGGAATTCAAATAAAATGCAGCCAAACATCAAACCTTAACGTCCCAAGTTCTCATCTTGGAGGAATAATGGAAAAAGAGGAAACCAGGAATATCCTCCTCTGCAACCAAACAGAGCACATAACCATTGGTACTAAATGTCCAAAAAAGTTCCCTTTATACTTCGATCACCTATCAAAGTCTTTAGTGTTACCTGACTAATAGGCGATCTATAGTTTGTCATTCGATTTTCTCTTAATCTCAATGTCTCTGTACAAATAATTAAACGCTGTTTTACTATTCTTCCTCACTGTTGATTCTAACCGATAGATCTTTAGATTTAGCTGATAAAATGTAATACTAACAGATACCTCCTGGTACCTTTTCAGGTTTGAAGACAAAGACTGGCAAGACACTGAAGGTTCTCATTCAGTTATATTCTACTTTCTCTTCAGAATTTGTCTCTGTATTGTTGGAATTCTTGCTAAAGGCGATGAGGTCATCAGACTTGGCATTTCCCAAAAGTTCCAAAAAATCTAGCAGCTCTGTATTGGATGGTTGGATGCTTACGGTTGCTAAGATCTCAAATAAGGAACCAGAATTGCTTTTAGATCTGCTTGAGGCAGTTCTTGAGAAGATTAAGACTCAAGTAGCCTTGGAATATGGTATGATTAAATTTCAACTTTAGACTTTACAGCCTTGTCCTTTTCTAATTTTCTGATCCTTGAGGCATATAATATACTTGGCCAGTGACCGGGTATAACATTACTTTTAGGAGATAGCTTTTGGACAATTATAGAACTACACGTATAACATATTTTTATTGGGAACGATTTACTTCCTTTTATAATCTATTGATTCTATCTTTGTTTTTATATTTTGATTTAGTTTGATTAATGATACCGGTTTGCTAACAAGTTTGTTTCTTCCCCCACTCTTCACGCTGCTCTTTTCGTGTGAGCATGGTTTTCTTATTTTCTTTTTCTGGTTCTTTCAGAAAGTCAGTACTTCTTGCCCTCAGACTACAAAATGGAAAGCTGTCATCAAGTGGCACAACTTTCCTCTTTGTTTGCATGGCTCGTTGACAAACTTAATGGAATAAAACTTAAGCGCAAGGGAGAAGTCTTTTCCGGGAAACACATTTCGAAAGGTGTTCTCATGGAGTTGCTACGTAAATGCCTTCTGGTTTCAGCTCCTTGGAACAAGCGGCTTCTGGATTCAGCCACCAATCTTGCACAGTTATTGGACGACGATTCTCTAGTGAAGAAACTGAACAAGCTATCCTTTGTGAATGCACCGATTTCATCTATCCCAGCTGAAGAAAATCCCAACCAAACCGCATCTGAGAACAGTTTTAATCAGGCGGAGTCGCTCCGTGAAGCAAGTCGGAAACTAGAGACGATTAAACTCCGCAAAAGAACGAACAACATGGCATCAAATCCAGTAGACAGTAATGTTGGAGGTTCCAGTAGATGGACTGTGGTAAGTTCATGGACTCCATGTCCAATTGGTATGTTGCCTCGTTCAATTGGCTGTTCTGGTCGGCCTTCCGTTCTCGATCTCAATCCCGAGAACGAACCAGCTTCAGAAGAGCTGAGAAGCAAAGAGAAGTGTGAACTCAACAACTTTAGTCACAAGAGGGAAGCCAGTTCTGATATTCAGCATCTTGATAACTCCATTGTCAAGAAAATGAAGGAAACAAATGATAAATCTCAGTTCGAGACCGAAGATGTCGAGTCACCTGAAGGTATCAAAGGGCGTTTGTTGATAGGTGGAATATGGAAGAATGTTGGGGAAGAAGAATTATCTGCCATTCAATCTGCGGTAAGGATATTAATATAA

mRNA sequence

ATGGATTCGCTGTTAGGATTTGAAGAGGTGCCGGGAGAGAATGAAGAAGAGAATTTGTCGACGTACAGCCGCAAATTGGTACCCTGGTCAACTTGGAACGAATGGCTTTTCGTTAGTCAGTCACTCTTCTCTGACTCTCCAGACTCCGTCGCCGCCGCTCTGAGCAGAATATCAACATGGAGAAGCAGAGGATGTCTTCCAGTTGTCATTGAAGTTACAGCGTCGATTATCGAAATCCAACAAAAGGATCCTTACTTCATAAAGAACCAATCAATGGATGCTTCAGTTCGTGGTACAGAGAACCAATTAAATCTTGTTTCACTTTCAGAGGAAGCACTAGCCATGTTATATTGCATGGCCATTATGAGGCTTGTAAATGGTGTTGTCGAGAAGACACGTAAGAAAACAGAAGTTTCAATTGCCGTGGCAGCTGATGCTATTGGTTTACCCCGTTTACTGATTGATATTCGTCATGAGGGTTCTCATCGTGAACTTCCAGCTCTTCAGGTGGTTCGACATGCCTCAGCCAAGGCGCTTTGTTGGTTGAAATCTTACTATTGGGAACCTCAGGAGAAAGCTATTCCGTTTCAAGGTGATATAAGTACTTCCATTAGAAAGGAAATCAAATCCAAACTTCGTGGTTTGGCTTTCAATTTAGAAATGAAGCAAAATCCTCAGCTGGAATCTGCCCTGGTCAAGCCAAAGCGTTTGAAGACAAAGACTGGCAAGACACTGAAGGTTCTCATTCAGTTATATTCTACTTTCTCTTCAGAATTTGTCTCTGTATTGTTGGAATTCTTGCTAAAGGCGATGAGGTCATCAGACTTGGCATTTCCCAAAAGTTCCAAAAAATCTAGCAGCTCTGTATTGGATGGTTGGATGCTTACGGTTGCTAAGATCTCAAATAAGGAACCAGAATTGCTTTTAGATCTGCTTGAGGCAGTTCTTGAGAAGATTAAGACTCAAGTAGCCTTGGAATATGAAAGTCAGTACTTCTTGCCCTCAGACTACAAAATGGAAAGCTGTCATCAAGTGGCACAACTTTCCTCTTTGTTTGCATGGCTCGTTGACAAACTTAATGGAATAAAACTTAAGCGCAAGGGAGAAGTCTTTTCCGGGAAACACATTTCGAAAGGTGTTCTCATGGAGTTGCTACGTAAATGCCTTCTGGTTTCAGCTCCTTGGAACAAGCGGCTTCTGGATTCAGCCACCAATCTTGCACAGTTATTGGACGACGATTCTCTAGTGAAGAAACTGAACAAGCTATCCTTTGTGAATGCACCGATTTCATCTATCCCAGCTGAAGAAAATCCCAACCAAACCGCATCTGAGAACAGTTTTAATCAGGCGGAGTCGCTCCGTGAAGCAAGTCGGAAACTAGAGACGATTAAACTCCGCAAAAGAACGAACAACATGGCATCAAATCCAGTAGACAGTAATGTTGGAGGTTCCAGTAGATGGACTGTGGTAAGTTCATGGACTCCATGTCCAATTGGTATGTTGCCTCGTTCAATTGGCTGTTCTGGTCGGCCTTCCGTTCTCGATCTCAATCCCGAGAACGAACCAGCTTCAGAAGAGCTGAGAAGCAAAGAGAAGTGTGAACTCAACAACTTTAGTCACAAGAGGGAAGCCAGTTCTGATATTCAGCATCTTGATAACTCCATTGTCAAGAAAATGAAGGAAACAAATGATAAATCTCAGTTCGAGACCGAAGATGTCGAGTCACCTGAAGGTATCAAAGGGCGTTTGTTGATAGGTGGAATATGGAAGAATGTTGGGGAAGAAGAATTATCTGCCATTCAATCTGCGGTAAGGATATTAATATAA

Coding sequence (CDS)

ATGGATTCGCTGTTAGGATTTGAAGAGGTGCCGGGAGAGAATGAAGAAGAGAATTTGTCGACGTACAGCCGCAAATTGGTACCCTGGTCAACTTGGAACGAATGGCTTTTCGTTAGTCAGTCACTCTTCTCTGACTCTCCAGACTCCGTCGCCGCCGCTCTGAGCAGAATATCAACATGGAGAAGCAGAGGATGTCTTCCAGTTGTCATTGAAGTTACAGCGTCGATTATCGAAATCCAACAAAAGGATCCTTACTTCATAAAGAACCAATCAATGGATGCTTCAGTTCGTGGTACAGAGAACCAATTAAATCTTGTTTCACTTTCAGAGGAAGCACTAGCCATGTTATATTGCATGGCCATTATGAGGCTTGTAAATGGTGTTGTCGAGAAGACACGTAAGAAAACAGAAGTTTCAATTGCCGTGGCAGCTGATGCTATTGGTTTACCCCGTTTACTGATTGATATTCGTCATGAGGGTTCTCATCGTGAACTTCCAGCTCTTCAGGTGGTTCGACATGCCTCAGCCAAGGCGCTTTGTTGGTTGAAATCTTACTATTGGGAACCTCAGGAGAAAGCTATTCCGTTTCAAGGTGATATAAGTACTTCCATTAGAAAGGAAATCAAATCCAAACTTCGTGGTTTGGCTTTCAATTTAGAAATGAAGCAAAATCCTCAGCTGGAATCTGCCCTGGTCAAGCCAAAGCGTTTGAAGACAAAGACTGGCAAGACACTGAAGGTTCTCATTCAGTTATATTCTACTTTCTCTTCAGAATTTGTCTCTGTATTGTTGGAATTCTTGCTAAAGGCGATGAGGTCATCAGACTTGGCATTTCCCAAAAGTTCCAAAAAATCTAGCAGCTCTGTATTGGATGGTTGGATGCTTACGGTTGCTAAGATCTCAAATAAGGAACCAGAATTGCTTTTAGATCTGCTTGAGGCAGTTCTTGAGAAGATTAAGACTCAAGTAGCCTTGGAATATGAAAGTCAGTACTTCTTGCCCTCAGACTACAAAATGGAAAGCTGTCATCAAGTGGCACAACTTTCCTCTTTGTTTGCATGGCTCGTTGACAAACTTAATGGAATAAAACTTAAGCGCAAGGGAGAAGTCTTTTCCGGGAAACACATTTCGAAAGGTGTTCTCATGGAGTTGCTACGTAAATGCCTTCTGGTTTCAGCTCCTTGGAACAAGCGGCTTCTGGATTCAGCCACCAATCTTGCACAGTTATTGGACGACGATTCTCTAGTGAAGAAACTGAACAAGCTATCCTTTGTGAATGCACCGATTTCATCTATCCCAGCTGAAGAAAATCCCAACCAAACCGCATCTGAGAACAGTTTTAATCAGGCGGAGTCGCTCCGTGAAGCAAGTCGGAAACTAGAGACGATTAAACTCCGCAAAAGAACGAACAACATGGCATCAAATCCAGTAGACAGTAATGTTGGAGGTTCCAGTAGATGGACTGTGGTAAGTTCATGGACTCCATGTCCAATTGGTATGTTGCCTCGTTCAATTGGCTGTTCTGGTCGGCCTTCCGTTCTCGATCTCAATCCCGAGAACGAACCAGCTTCAGAAGAGCTGAGAAGCAAAGAGAAGTGTGAACTCAACAACTTTAGTCACAAGAGGGAAGCCAGTTCTGATATTCAGCATCTTGATAACTCCATTGTCAAGAAAATGAAGGAAACAAATGATAAATCTCAGTTCGAGACCGAAGATGTCGAGTCACCTGAAGGTATCAAAGGGCGTTTGTTGATAGGTGGAATATGGAAGAATGTTGGGGAAGAAGAATTATCTGCCATTCAATCTGCGGTAAGGATATTAATATAA

Protein sequence

MDSLLGFEEVPGENEEENLSTYSRKLVPWSTWNEWLFVSQSLFSDSPDSVAAALSRISTWRSRGCLPVVIEVTASIIEIQQKDPYFIKNQSMDASVRGTENQLNLVSLSEEALAMLYCMAIMRLVNGVVEKTRKKTEVSIAVAADAIGLPRLLIDIRHEGSHRELPALQVVRHASAKALCWLKSYYWEPQEKAIPFQGDISTSIRKEIKSKLRGLAFNLEMKQNPQLESALVKPKRLKTKTGKTLKVLIQLYSTFSSEFVSVLLEFLLKAMRSSDLAFPKSSKKSSSSVLDGWMLTVAKISNKEPELLLDLLEAVLEKIKTQVALEYESQYFLPSDYKMESCHQVAQLSSLFAWLVDKLNGIKLKRKGEVFSGKHISKGVLMELLRKCLLVSAPWNKRLLDSATNLAQLLDDDSLVKKLNKLSFVNAPISSIPAEENPNQTASENSFNQAESLREASRKLETIKLRKRTNNMASNPVDSNVGGSSRWTVVSSWTPCPIGMLPRSIGCSGRPSVLDLNPENEPASEELRSKEKCELNNFSHKREASSDIQHLDNSIVKKMKETNDKSQFETEDVESPEGIKGRLLIGGIWKNVGEEELSAIQSAVRILI*
Homology
BLAST of Chy5G092220 vs. ExPASy Swiss-Prot
Match: Q9Y4W2 (Ribosomal biogenesis protein LAS1L OS=Homo sapiens OX=9606 GN=LAS1L PE=1 SV=2)

HSP 1 Score: 75.1 bits (183), Expect = 3.1e-12
Identity = 50/164 (30.49%), Postives = 78/164 (47.56%), Query Frame = 0

Query: 26  LVPWSTWNEWLFVSQSLFSDSPDSVAAALSRISTWRSRGC--LPVVIEVTASIIEIQQKD 85
           +V W +  EW  V+  LF D       AL+RI+ WRSR    LP+ +  TA +I  +  D
Sbjct: 42  VVAWLSRAEWDQVTVYLFCDDHKLQRYALNRITVWRSRSGNELPLAVASTADLIRCKLLD 101

Query: 86  PYFIKNQSMDASVRGTENQLNLVSLSEEALAMLYCMAIMRLVNGVVEKTRKKTEVSIAVA 145
                       V G         L  + L +LY MA++R VN + E+  K  +V +   
Sbjct: 102 ------------VTG--------GLGTDELRLLYGMALVRFVNLISERKTKFAKVPLKCL 161

Query: 146 ADAIGLPRLLIDIRHEGSHRELPALQVVRHASAKALCWLKSYYW 188
           A  + +P  ++D+RHE +H+++P +   R      L WL+  YW
Sbjct: 162 AQEVNIPDWIVDLRHELTHKKMPHINDCRRGCYFVLDWLQKTYW 185

BLAST of Chy5G092220 vs. ExPASy Swiss-Prot
Match: A2BE28 (Ribosomal biogenesis protein LAS1L OS=Mus musculus OX=10090 GN=Las1l PE=1 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 2.0e-11
Identity = 51/180 (28.33%), Postives = 84/180 (46.67%), Query Frame = 0

Query: 13  ENEEENLSTYSRKLVPWSTWNEWLFVSQSLFSDSPDSVAAALSRISTWRSR--GCLPVVI 72
           EN+ E+ S     +V W +  EW  V+  LF D       AL+RI+ WRSR    LP+ +
Sbjct: 15  ENQPESPSARG-IVVSWLSRAEWEQVTVYLFCDDHKLQQYALNRITVWRSRLGNELPLAV 74

Query: 73  EVTASIIEIQQKDPYFIKNQSMDASVRGTENQLNLVSLSEEALAMLYCMAIMRLVNGVVE 132
             TA ++  +  D              GT        L  + L +LY MA++R VN + E
Sbjct: 75  ASTADLVRCKLID------------AAGT--------LGTDELRLLYGMALVRFVNLISE 134

Query: 133 KTRKKTEVSIAVAADAIGLPRLLIDIRHEGSHRELPALQVVRHASAKALCWLKSYYWEPQ 191
           +  K + + +   A  + +P  ++++RH  +H+++P +   R      L WL+  YW  Q
Sbjct: 135 RKTKCSNLPLKYLAQEVNIPDWIVELRHNLTHKKMPHINECRRGCYFVLNWLQKTYWSRQ 173

BLAST of Chy5G092220 vs. ExPASy Swiss-Prot
Match: P36146 (Protein LAS1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=LAS1 PE=1 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 3.7e-10
Identity = 51/178 (28.65%), Postives = 87/178 (48.88%), Query Frame = 0

Query: 25  KLVPWSTWNE------WLFVSQSLFSDSPDSVAAALSRISTWRSRGC--LPVVIEVTASI 84
           ++VPW  + E      W +          D    A+ R+ ++R +G   LP V++ TA I
Sbjct: 5   RIVPWRDFAELEELKLWFYPKSK--GTIEDKRQRAVQRVQSYRLKGSQYLPHVVDSTAQI 64

Query: 85  IEIQQKDPYFIKNQSMDASVRGTENQLNL-VSLSEEALAMLYCMAIMRLVNGVVEKTRK- 144
                             +V   E +  L V      + + Y MA++R VNG+++ T++ 
Sbjct: 65  ----------------TCAVLLDEKEACLGVHQDSIPIRLSYVMALIRFVNGLLDPTQQS 124

Query: 145 KTEVSIAVAADAIGLPRLLIDIRHEGSH-RELPALQVVRHASAKALCWLKSYYWEPQE 192
           +  + +   A  IGLP   +D+RH G+H R+LP L+++R A+ +AL WL  +YW  +E
Sbjct: 125 QFAIPLHTLAAKIGLPSWFVDLRHWGTHERDLPGLEMLRWAANEALSWLYDHYWNDEE 164

BLAST of Chy5G092220 vs. ExPASy Swiss-Prot
Match: O42936 (Pre-rRNA-processing protein las1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=las1 PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 3.5e-08
Identity = 105/517 (20.31%), Postives = 207/517 (40.04%), Query Frame = 0

Query: 25  KLVPWSTWNEWLFVSQSLFSDSPDSV--AAALSR----ISTWRSRGCLPVVIEVTASIIE 84
           K+VPW    ++L++    +++  + +   AAL R    I  W +RG +P  +E T+ ++ 
Sbjct: 4   KVVPWRLKEDFLYLMSCFYNEGQEGIPDLAALFRGVEIIQAWSTRGRIPHSVESTSQLVS 63

Query: 85  IQQKDPYFIKNQSMDASVRGTENQLNLVSLSEEALAMLYCMAIMRLVNGVVEKTRK-KTE 144
                                    +L+S ++ +  +   ++I R V+G+++  ++ +  
Sbjct: 64  -------------------------SLIS-NDRSQKLALAVSISRFVSGLLDPIQQSQYA 123

Query: 145 VSIAVAADAIGLPRLLIDIRHEGSHRELPALQVVRHASAKALCWLKSYYWEP---QEKAI 204
           + +AV A +I LP   +++RH  +H ELP+L V+R A+ +AL WL  +YW P    E   
Sbjct: 124 IPMAVLAKSIDLPTYFVELRHAITHEELPSLPVLRQAAQRALSWLYDHYWNPAATAESED 183

Query: 205 PFQGDISTSIRK-EIKSKLRGLAFNLEMKQNPQLESALVKPKRLKTKTGKTLKVLIQLYS 264
            +    +  + K EIK K++ L       +   + + +  P        +   ++ +L +
Sbjct: 184 TYDYTETNELHKFEIKRKVKDLLKQWRSWRKVNVSANMFLPVEEHDYIQQFEALVQELVT 243

Query: 265 T-------FSSEFV-----------SVLLEFLLKAMRSSDLAFPKSSKKSS--SSVLDGW 324
           T         + FV           +  L+ ++       +  P  +   S    + + W
Sbjct: 244 TANLRLPYIPASFVDDEKDLDFAIETTNLDIVVSCFLEKRVLIPSRTMPISFFPKLKNVW 303

Query: 325 MLTVAKISNKEPELLLDLLEAVLEKIKTQVALEYESQYFLPSDYKMESCHQVAQLSSLFA 384
           +  +  I++K    L  L   +  +I  +++   +S  FL    K E   + +    L  
Sbjct: 304 LPLLQSIASKHSFFLPALFTTLWSEI-LEISQTVDSLVFLDLKEKEELTDKESAGCYLAK 363

Query: 385 WLVDKLNGIKLKRKGEVFSGK------HISKGVLMELLRKCLLVSAPWNKRLLDSATNLA 444
           W    +         E ++G+       +++  L  +L  CL  S P+ K ++D  + L 
Sbjct: 364 WYAYLMR--------EAYTGQPWTSTLSVTQTDLASVLEICLQRSDPFTKIIIDELSILD 423

Query: 445 QLLDDDSLVKKLNKLSFVNAPISSIPAEENPNQTASENSFNQAESLREASRKLETIKLRK 504
           + L+        NK S    P+    ++   ++   E  F   E +     K E  K   
Sbjct: 424 KELE--------NKFS----PLFQYRSDMFDSRILDEQEF---EDITLEQMKTELNKFSV 470

BLAST of Chy5G092220 vs. ExPASy TrEMBL
Match: A0A0A0KI89 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G430640 PE=4 SV=1)

HSP 1 Score: 1119.4 bits (2894), Expect = 0.0e+00
Identity = 586/608 (96.38%), Postives = 595/608 (97.86%), Query Frame = 0

Query: 1   MDSLLGFEEVPGENEEENLSTYSRKLVPWSTWNEWLFVSQSLFSDSPDSVAAALSRISTW 60
           MDSLLGFEEVPGENEEENLSTYSRKLVPWSTWNEWLFVSQSLFSDSPDSVAAALSRISTW
Sbjct: 127 MDSLLGFEEVPGENEEENLSTYSRKLVPWSTWNEWLFVSQSLFSDSPDSVAAALSRISTW 186

Query: 61  RSRGCLPVVIEVTASIIEIQQKDPYFIKNQSMDASVRGTENQLNLVSLSEEALAMLYCMA 120
           RS+GCLPVVIEVTASIIEIQQKDPYFIKNQSMDASVRGTENQLNLVSLSEEALAMLYCMA
Sbjct: 187 RSKGCLPVVIEVTASIIEIQQKDPYFIKNQSMDASVRGTENQLNLVSLSEEALAMLYCMA 246

Query: 121 IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRLLIDIRHEGSHRELPALQVVRHASAKALC 180
           IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRLLIDIRHEGSHRELPALQ+VRHASAKALC
Sbjct: 247 IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRLLIDIRHEGSHRELPALQMVRHASAKALC 306

Query: 181 WLKSYYWEPQEKAIPFQGDISTSIRKEIKSKLRGLAFNLEMKQNPQLESALVKPKRLKTK 240
           WLKSYYWEPQEKAIPFQGDISTSIRKEIKSKLRGLAFNLEMKQNPQLESA+VKPKRLKTK
Sbjct: 307 WLKSYYWEPQEKAIPFQGDISTSIRKEIKSKLRGLAFNLEMKQNPQLESAVVKPKRLKTK 366

Query: 241 TGKTLKVLIQLYSTFSSEFVSVLLEFLLKAMRSSDLAFPKSSKKSSSSVLDGWMLTVAKI 300
           TGKTLKVLIQLYSTFSSEFVSVLLEFLLKAMRSSDLAFPK+SKKSSSSVLDGW LTVAKI
Sbjct: 367 TGKTLKVLIQLYSTFSSEFVSVLLEFLLKAMRSSDLAFPKNSKKSSSSVLDGWKLTVAKI 426

Query: 301 SNKEPELLLDLLEAVLEKIKTQVALEYESQYFLPSDYKMESCHQVAQLSSLFAWLVDKLN 360
           SNKEPELLLDLLEAVLEKIKTQVALEYESQYFLPSD+KMESC QVAQLSSLFAWLVDKLN
Sbjct: 427 SNKEPELLLDLLEAVLEKIKTQVALEYESQYFLPSDHKMESC-QVAQLSSLFAWLVDKLN 486

Query: 361 GIKLKRKGEVFSGKHISKGVLMELLRKCLLVSAPWNKRLLDSATNLAQLLDDDSLVKKLN 420
           GIKLKRKGEVFSGKHI KGVLMELLRKCLLVSAPWNKRLLDSATNLAQLLDDDSLVKKLN
Sbjct: 487 GIKLKRKGEVFSGKHIPKGVLMELLRKCLLVSAPWNKRLLDSATNLAQLLDDDSLVKKLN 546

Query: 421 KLSFVNAPISSIPAEENPNQTASENSFNQAESLREASRKLETIKLRKRTNNMASNPVDSN 480
           KL FVNAPISSIP EENPN +ASE+SFNQAESLREASRKLETIKLRKR NN+ASNPVD N
Sbjct: 547 KLFFVNAPISSIPDEENPNHSASEDSFNQAESLREASRKLETIKLRKRKNNLASNPVDGN 606

Query: 481 VGGSSRWTVVSSWTPCPIGMLPRSIGCSGRPSVLDLNPENEPASEELRSKEKCELNNFSH 540
           VGGSSRWTVVSSWTPCPIGMLPRSIGCSGRPSVLDLNPENEP SEELRSKEKCELNN S 
Sbjct: 607 VGGSSRWTVVSSWTPCPIGMLPRSIGCSGRPSVLDLNPENEPTSEELRSKEKCELNNSSQ 666

Query: 541 KREASSDIQHLDNSIVKKMKETNDKSQFETEDVESPEGIKGRLLIGGIWKNVGEEELSAI 600
           KREASSDI HLDNSIVKKMK+TNDKSQ ETEDVESPEGIKGRLLIGGIWKNVGEEELSAI
Sbjct: 667 KREASSDIHHLDNSIVKKMKDTNDKSQLETEDVESPEGIKGRLLIGGIWKNVGEEELSAI 726

Query: 601 QSAVRILI 609
           QSAVRILI
Sbjct: 727 QSAVRILI 733

BLAST of Chy5G092220 vs. ExPASy TrEMBL
Match: A0A1S3BNF1 (pre-rRNA-processing protein las1 OS=Cucumis melo OX=3656 GN=LOC103491463 PE=4 SV=1)

HSP 1 Score: 1082.4 bits (2798), Expect = 0.0e+00
Identity = 569/608 (93.59%), Postives = 585/608 (96.22%), Query Frame = 0

Query: 1   MDSLLGFEEVPGENEEENLSTYSRKLVPWSTWNEWLFVSQSLFSDSPDSVAAALSRISTW 60
           MDSLLGFEEV GENEEENLSTYSRKLVPWSTWNEWLFVSQSLFSDSPD VAAALSRISTW
Sbjct: 1   MDSLLGFEEVLGENEEENLSTYSRKLVPWSTWNEWLFVSQSLFSDSPDFVAAALSRISTW 60

Query: 61  RSRGCLPVVIEVTASIIEIQQKDPYFIKNQSMDASVRGTENQLNLVSLSEEALAMLYCMA 120
           RSRGCLPVV+EVTASIIEIQQKDPYFIKNQS+DASVRGTENQLN+VSLSEEALAMLYCMA
Sbjct: 61  RSRGCLPVVVEVTASIIEIQQKDPYFIKNQSIDASVRGTENQLNIVSLSEEALAMLYCMA 120

Query: 121 IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRLLIDIRHEGSHRELPALQVVRHASAKALC 180
           IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRLLIDIRHEGSHRELPALQVVRHASAKALC
Sbjct: 121 IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRLLIDIRHEGSHRELPALQVVRHASAKALC 180

Query: 181 WLKSYYWEPQEKAIPFQGDISTSIRKEIKSKLRGLAFNLEMKQNPQLESALVKPKRLKTK 240
           WLKSYYWEPQEKAIPFQGDISTSIRKEIKSKLR LAFNLE+KQNPQLESALVKPKRLKTK
Sbjct: 181 WLKSYYWEPQEKAIPFQGDISTSIRKEIKSKLRVLAFNLEVKQNPQLESALVKPKRLKTK 240

Query: 241 TGKTLKVLIQLYSTFSSEFVSVLLEFLLKAMRSSDLAFPKSSKKSSSSVLDGWMLTVAKI 300
           TGKT+KVLIQLYSTF SEFVSVLLEFLLKAMRSSDLAFPK+SKKSSSSVLDGW LTVAKI
Sbjct: 241 TGKTMKVLIQLYSTFPSEFVSVLLEFLLKAMRSSDLAFPKNSKKSSSSVLDGWKLTVAKI 300

Query: 301 SNKEPELLLDLLEAVLEKIKTQVALEYESQYFLPSDYKMESCHQVAQLSSLFAWLVDKLN 360
           SNKEPELLLDLLEAVLEKIKTQVALEYESQYF+PSD+K+ESC QV QLSSLFAWLVDKLN
Sbjct: 301 SNKEPELLLDLLEAVLEKIKTQVALEYESQYFMPSDHKVESC-QVVQLSSLFAWLVDKLN 360

Query: 361 GIKLKRKGEVFSGKHISKGVLMELLRKCLLVSAPWNKRLLDSATNLAQLLDDDSLVKKLN 420
           GIKLKRKGEVFSGKHI KG+LMELLRKCLLVSAPWNKRLLDSAT+LAQLLDDDSLVKKLN
Sbjct: 361 GIKLKRKGEVFSGKHIPKGILMELLRKCLLVSAPWNKRLLDSATDLAQLLDDDSLVKKLN 420

Query: 421 KLSFVNAPISSIPAEENPNQTASENSFNQAESLREASRKLETIKLRKRTNNMASNPVDSN 480
           KLSFVN PIS IP  ENPNQTASENSFNQAESLREASRKLE IKLRKR NN+ASNPVD N
Sbjct: 421 KLSFVNVPISPIPDGENPNQTASENSFNQAESLREASRKLEMIKLRKRKNNLASNPVDGN 480

Query: 481 VGGSSRWTVVSSWTPCPIGMLPRSIGCSGRPSVLDLNPENEPASEELRSKEKCELNNFSH 540
           VG SSRWTVVSSWTPCPIGMLPRSIGCSGR SVLDLNPENEPASEE RSK+   LNN SH
Sbjct: 481 VGCSSRWTVVSSWTPCPIGMLPRSIGCSGRLSVLDLNPENEPASEEPRSKDNRALNNSSH 540

Query: 541 KREASSDIQHLDNSIVKKMKETNDKSQFETEDVESPEGIKGRLLIGGIWKNVGEEELSAI 600
           KREASSDIQHLD+SIVKKMKETNDKSQFETEDVESPEGI+GRLLIGGIWK VGEEELSAI
Sbjct: 541 KREASSDIQHLDSSIVKKMKETNDKSQFETEDVESPEGIEGRLLIGGIWKKVGEEELSAI 600

Query: 601 QSAVRILI 609
           QS+VRILI
Sbjct: 601 QSSVRILI 607

BLAST of Chy5G092220 vs. ExPASy TrEMBL
Match: A0A5A7V7Q2 (Pre-rRNA-processing protein las1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold212G001390 PE=4 SV=1)

HSP 1 Score: 1075.8 bits (2781), Expect = 0.0e+00
Identity = 572/631 (90.65%), Postives = 587/631 (93.03%), Query Frame = 0

Query: 1   MDSLLGFEEVPGENEEENLSTYSRKLVPWSTWNEWLFVSQSLFSDSPDSVAAALSR---- 60
           MDSLLGFEEV GENEEENLSTYSRKLVPWSTWNEWLFVSQSLFSDSPDSVAAALSR    
Sbjct: 1   MDSLLGFEEVLGENEEENLSTYSRKLVPWSTWNEWLFVSQSLFSDSPDSVAAALSRVSLL 60

Query: 61  -------------------ISTWRSRGCLPVVIEVTASIIEIQQKDPYFIKNQSMDASVR 120
                              ISTWRSRGCLPVV+EVTASIIEIQQKDPYFIKNQS+DASVR
Sbjct: 61  ISAACLCSVVLTFEVALGPISTWRSRGCLPVVVEVTASIIEIQQKDPYFIKNQSIDASVR 120

Query: 121 GTENQLNLVSLSEEALAMLYCMAIMRLVNGVVEKTRKKTEVSIAVAADAIGLPRLLIDIR 180
           GTENQLN+VSLSEEALAMLYCMAIMRLVNGVVEKTRKKTEVSIAVAADAIGLPRLLIDIR
Sbjct: 121 GTENQLNIVSLSEEALAMLYCMAIMRLVNGVVEKTRKKTEVSIAVAADAIGLPRLLIDIR 180

Query: 181 HEGSHRELPALQVVRHASAKALCWLKSYYWEPQEKAIPFQGDISTSIRKEIKSKLRGLAF 240
           HEGSHRELPALQVVRHASAKALCWLKSYYWEPQEKAIPFQGDISTSIRKEIKSKLR LAF
Sbjct: 181 HEGSHRELPALQVVRHASAKALCWLKSYYWEPQEKAIPFQGDISTSIRKEIKSKLRVLAF 240

Query: 241 NLEMKQNPQLESALVKPKRLKTKTGKTLKVLIQLYSTFSSEFVSVLLEFLLKAMRSSDLA 300
           NLE+KQNPQLESALVKPKRLKTKTGK +KVLIQLYSTFSSEFVSVLLEFLLKAMRSSDLA
Sbjct: 241 NLEVKQNPQLESALVKPKRLKTKTGKIMKVLIQLYSTFSSEFVSVLLEFLLKAMRSSDLA 300

Query: 301 FPKSSKKSSSSVLDGWMLTVAKISNKEPELLLDLLEAVLEKIKTQVALEYESQYFLPSDY 360
           FPK+SKKSSSSVLDGW LTVAKISNKEPELLLDLLEAVLEKIKTQVALEYESQYF+PSD+
Sbjct: 301 FPKNSKKSSSSVLDGWKLTVAKISNKEPELLLDLLEAVLEKIKTQVALEYESQYFMPSDH 360

Query: 361 KMESCHQVAQLSSLFAWLVDKLNGIKLKRKGEVFSGKHISKGVLMELLRKCLLVSAPWNK 420
           K+ESC QV QLSSLFAWLVDKLNGIKLKRKGEVFSGKHI KG+LMELLRKCLLVSAPWNK
Sbjct: 361 KVESC-QVVQLSSLFAWLVDKLNGIKLKRKGEVFSGKHIPKGILMELLRKCLLVSAPWNK 420

Query: 421 RLLDSATNLAQLLDDDSLVKKLNKLSFVNAPISSIPAEENPNQTASENSFNQAESLREAS 480
           RLLDSAT+LAQLLDDDSLVKKLNKLSFVN PIS IP  ENPNQTASENSFNQAESLREAS
Sbjct: 421 RLLDSATDLAQLLDDDSLVKKLNKLSFVNVPISPIPDGENPNQTASENSFNQAESLREAS 480

Query: 481 RKLETIKLRKRTNNMASNPVDSNVGGSSRWTVVSSWTPCPIGMLPRSIGCSGRPSVLDLN 540
           RKLE IKLRKR NN+ASNPVD NVGGSSRWTVVSSWTPCPIGMLPRSIGCSGR SVLDLN
Sbjct: 481 RKLEMIKLRKRKNNLASNPVDGNVGGSSRWTVVSSWTPCPIGMLPRSIGCSGRLSVLDLN 540

Query: 541 PENEPASEELRSKEKCELNNFSHKREASSDIQHLDNSIVKKMKETNDKSQFETEDVESPE 600
           PENEPASEE RSKE   LNN SHKREASSDIQHLD+SIVKKMKETNDKSQFETEDVESPE
Sbjct: 541 PENEPASEEPRSKENRALNNSSHKREASSDIQHLDSSIVKKMKETNDKSQFETEDVESPE 600

Query: 601 GIKGRLLIGGIWKNVGEEELSAIQSAVRILI 609
           GI+GRLLIGGIWK VGEEELSAIQS+VRILI
Sbjct: 601 GIEGRLLIGGIWKKVGEEELSAIQSSVRILI 630

BLAST of Chy5G092220 vs. ExPASy TrEMBL
Match: A0A5D3B9Y3 (Pre-rRNA-processing protein las1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G00650 PE=4 SV=1)

HSP 1 Score: 1069.3 bits (2764), Expect = 5.9e-309
Identity = 569/631 (90.17%), Postives = 585/631 (92.71%), Query Frame = 0

Query: 1   MDSLLGFEEVPGENEEENLSTYSRKLVPWSTWNEWLFVSQSLFSDSPDSVAAALSR---- 60
           MDSLLGFEEV GENEEENLSTYSRKLVPWSTWNEWLFVSQSLFSDSPD VAAALSR    
Sbjct: 1   MDSLLGFEEVLGENEEENLSTYSRKLVPWSTWNEWLFVSQSLFSDSPDFVAAALSRVSLL 60

Query: 61  -------------------ISTWRSRGCLPVVIEVTASIIEIQQKDPYFIKNQSMDASVR 120
                              ISTWRSRGCLPVV+EVTASIIEIQQKDPYFIKNQS+DASVR
Sbjct: 61  ISAACLCSVVLTFEVALGPISTWRSRGCLPVVVEVTASIIEIQQKDPYFIKNQSIDASVR 120

Query: 121 GTENQLNLVSLSEEALAMLYCMAIMRLVNGVVEKTRKKTEVSIAVAADAIGLPRLLIDIR 180
           GTENQLN+VSLSEEALAMLYCMAIMRLVNGVVEKTRKKTEVSIAVAADAIGLPRLLIDIR
Sbjct: 121 GTENQLNIVSLSEEALAMLYCMAIMRLVNGVVEKTRKKTEVSIAVAADAIGLPRLLIDIR 180

Query: 181 HEGSHRELPALQVVRHASAKALCWLKSYYWEPQEKAIPFQGDISTSIRKEIKSKLRGLAF 240
           HEGSHRELPALQVVRHASAKALCWLKSYYWEPQEKAIPFQGDISTSIRKEIKSKLR LAF
Sbjct: 181 HEGSHRELPALQVVRHASAKALCWLKSYYWEPQEKAIPFQGDISTSIRKEIKSKLRVLAF 240

Query: 241 NLEMKQNPQLESALVKPKRLKTKTGKTLKVLIQLYSTFSSEFVSVLLEFLLKAMRSSDLA 300
           NLE+KQNPQLESALVKPKRLKTKTGKT+KVLIQLYSTF SEFVSVLLEFLLKAMRSSDLA
Sbjct: 241 NLEVKQNPQLESALVKPKRLKTKTGKTMKVLIQLYSTFPSEFVSVLLEFLLKAMRSSDLA 300

Query: 301 FPKSSKKSSSSVLDGWMLTVAKISNKEPELLLDLLEAVLEKIKTQVALEYESQYFLPSDY 360
           FPK+SKKSSSSVLDGW LTVAKISNKEPELLLDLLEAVLEKIKTQVALEYESQYF+PSD+
Sbjct: 301 FPKNSKKSSSSVLDGWKLTVAKISNKEPELLLDLLEAVLEKIKTQVALEYESQYFMPSDH 360

Query: 361 KMESCHQVAQLSSLFAWLVDKLNGIKLKRKGEVFSGKHISKGVLMELLRKCLLVSAPWNK 420
           K+ESC QV QLSSLFAWLVDKLNGIKLKRKGEVFSGKHI KG+LMELLRKCLLVSAPWNK
Sbjct: 361 KVESC-QVVQLSSLFAWLVDKLNGIKLKRKGEVFSGKHIPKGILMELLRKCLLVSAPWNK 420

Query: 421 RLLDSATNLAQLLDDDSLVKKLNKLSFVNAPISSIPAEENPNQTASENSFNQAESLREAS 480
           RLLDSAT+LAQLLDDDSLVKKLNKLSFVN PIS IP  ENPNQTASENSFNQAESLREAS
Sbjct: 421 RLLDSATDLAQLLDDDSLVKKLNKLSFVNVPISPIPDGENPNQTASENSFNQAESLREAS 480

Query: 481 RKLETIKLRKRTNNMASNPVDSNVGGSSRWTVVSSWTPCPIGMLPRSIGCSGRPSVLDLN 540
           RKLE IKLRKR NN+ASNPVD NVG SSRWTVVSSWTPCPIGMLPRSIGCSGR SVLDLN
Sbjct: 481 RKLEMIKLRKRKNNLASNPVDGNVGCSSRWTVVSSWTPCPIGMLPRSIGCSGRLSVLDLN 540

Query: 541 PENEPASEELRSKEKCELNNFSHKREASSDIQHLDNSIVKKMKETNDKSQFETEDVESPE 600
           PENEPASEE RSK+   LNN SHKREASSDIQHLD+SIVKKMKETNDKSQFETEDVESPE
Sbjct: 541 PENEPASEEPRSKDNRALNNSSHKREASSDIQHLDSSIVKKMKETNDKSQFETEDVESPE 600

Query: 601 GIKGRLLIGGIWKNVGEEELSAIQSAVRILI 609
           GI+GRLLIGGIWK VGEEELSAIQS+VRILI
Sbjct: 601 GIEGRLLIGGIWKKVGEEELSAIQSSVRILI 630

BLAST of Chy5G092220 vs. ExPASy TrEMBL
Match: A0A6J1H0C5 (uncharacterized protein LOC111459262 OS=Cucurbita moschata OX=3662 GN=LOC111459262 PE=4 SV=1)

HSP 1 Score: 948.0 bits (2449), Expect = 2.0e-272
Identity = 505/608 (83.06%), Postives = 542/608 (89.14%), Query Frame = 0

Query: 1   MDSLLGFEEVPGENEEENLSTYSRKLVPWSTWNEWLFVSQSLFSDSPDSVAAALSRISTW 60
           M+SLLGFEEVP E EE+N STYSRKLVPWSTW+EWLFVSQSLFSDS DSVAAAL RISTW
Sbjct: 1   MESLLGFEEVPVETEEDNSSTYSRKLVPWSTWSEWLFVSQSLFSDSSDSVAAALGRISTW 60

Query: 61  RSRGCLPVVIEVTASIIEIQQKDPYFIKNQSMDASVRGTENQLNLVSLSEEALAMLYCMA 120
           RSRGCLPVV+EVTASIIEIQQKDPYFIKNQS+DASVRGTE QLN VSLSEEALAMLYCMA
Sbjct: 61  RSRGCLPVVVEVTASIIEIQQKDPYFIKNQSVDASVRGTEEQLNSVSLSEEALAMLYCMA 120

Query: 121 IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRLLIDIRHEGSHRELPALQVVRHASAKALC 180
           IMRLVNGVVEKTRKKTEVSIAVAADAIGLPR+LIDIRHEGSHRELPALQVVR AS KAL 
Sbjct: 121 IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRVLIDIRHEGSHRELPALQVVRCASIKALH 180

Query: 181 WLKSYYWEPQEKAIPFQGDISTSIRKEIKSKLRGLAFNLEMKQNPQLESALVKPKRLKTK 240
           WLK+YYW+PQEKAIPF GDIST+IRKEIKS+LR LAFNL  KQ+ Q   ++VKPKRLKTK
Sbjct: 181 WLKAYYWDPQEKAIPFLGDISTTIRKEIKSRLRDLAFNLSEKQDSQ-RGSVVKPKRLKTK 240

Query: 241 TGKTLKVLIQLYSTFSSEFVSVLLEFLLKAMRSSDLAFPKSSKKSSSSVLDGWMLTVAKI 300
           TGKTLK LIQLYSTFSSEF+SVLLEFLLKAMRSSDLAFP++SKKS S+VL+GW L +AKI
Sbjct: 241 TGKTLKFLIQLYSTFSSEFLSVLLEFLLKAMRSSDLAFPENSKKSRSAVLEGWKLAIAKI 300

Query: 301 SNKEPELLLDLLEAVLEKIKTQVALEYESQYFLPSDYKMESCHQVAQLSSLFAWLVDKLN 360
           SNKEPELLLDLLEA+LEKIKTQ ALEYESQYF  SD+KM+SC QVAQLSSLFAWLV+KL 
Sbjct: 301 SNKEPELLLDLLEAILEKIKTQEALEYESQYFTSSDHKMKSC-QVAQLSSLFAWLVEKLK 360

Query: 361 GIKLKRKGEVFSGKHISKGVLMELLRKCLLVSAPWNKRLLDSATNLAQLLDDDSLVKKLN 420
           GIKLKR GEVFSGKHI KGVLMELLRKCLLVSAP NKRL+DSAT+LAQLLDD SL+KKLN
Sbjct: 361 GIKLKRNGEVFSGKHIPKGVLMELLRKCLLVSAPGNKRLMDSATDLAQLLDDGSLMKKLN 420

Query: 421 KLSFVNAPISSIPAEENPNQTASENSFNQAESLREASRKLETIKLRKRTNNMASNPVDSN 480
           KLSF+N   S IP EENPNQT SEN+ N  ESL EASRKLE IKL KR NN+A+NPVD N
Sbjct: 421 KLSFLNLLNSDIPDEENPNQTTSENNLNHVESLHEASRKLEMIKLHKRKNNLAANPVDGN 480

Query: 481 VGGSSRWTVVSSWTPCPIGMLPRSIGCSGRPSVLDLNPENEPASEELRSKEKCELNNFSH 540
           VG S+RWTVV+SWTPCPIGMLPR IG SGR  VLDLNPENEPASE+LRSKE  EL   S 
Sbjct: 481 VGSSTRWTVVNSWTPCPIGMLPRPIGSSGRLPVLDLNPENEPASEDLRSKENIELGKCSL 540

Query: 541 KREASSDIQHLDNSIVKKMKETNDKSQFETEDVESPEGIKGRLLIGGIWKNVGEEELSAI 600
           KREASSD Q LD+  VKKMKETN   Q+E +DVES EG+KGRLLIGGIWK VGEEELSAI
Sbjct: 541 KREASSDTQQLDSGTVKKMKETNGNFQYEMDDVESREGVKGRLLIGGIWKKVGEEELSAI 600

Query: 601 QSAVRILI 609
           QSAVRILI
Sbjct: 601 QSAVRILI 606

BLAST of Chy5G092220 vs. NCBI nr
Match: XP_004140277.1 (pre-rRNA-processing protein las1 [Cucumis sativus] >XP_031744103.1 pre-rRNA-processing protein las1 [Cucumis sativus] >KAE8647368.1 hypothetical protein Csa_003855 [Cucumis sativus])

HSP 1 Score: 1119 bits (2894), Expect = 0.0
Identity = 586/608 (96.38%), Postives = 595/608 (97.86%), Query Frame = 0

Query: 1   MDSLLGFEEVPGENEEENLSTYSRKLVPWSTWNEWLFVSQSLFSDSPDSVAAALSRISTW 60
           MDSLLGFEEVPGENEEENLSTYSRKLVPWSTWNEWLFVSQSLFSDSPDSVAAALSRISTW
Sbjct: 1   MDSLLGFEEVPGENEEENLSTYSRKLVPWSTWNEWLFVSQSLFSDSPDSVAAALSRISTW 60

Query: 61  RSRGCLPVVIEVTASIIEIQQKDPYFIKNQSMDASVRGTENQLNLVSLSEEALAMLYCMA 120
           RS+GCLPVVIEVTASIIEIQQKDPYFIKNQSMDASVRGTENQLNLVSLSEEALAMLYCMA
Sbjct: 61  RSKGCLPVVIEVTASIIEIQQKDPYFIKNQSMDASVRGTENQLNLVSLSEEALAMLYCMA 120

Query: 121 IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRLLIDIRHEGSHRELPALQVVRHASAKALC 180
           IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRLLIDIRHEGSHRELPALQ+VRHASAKALC
Sbjct: 121 IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRLLIDIRHEGSHRELPALQMVRHASAKALC 180

Query: 181 WLKSYYWEPQEKAIPFQGDISTSIRKEIKSKLRGLAFNLEMKQNPQLESALVKPKRLKTK 240
           WLKSYYWEPQEKAIPFQGDISTSIRKEIKSKLRGLAFNLEMKQNPQLESA+VKPKRLKTK
Sbjct: 181 WLKSYYWEPQEKAIPFQGDISTSIRKEIKSKLRGLAFNLEMKQNPQLESAVVKPKRLKTK 240

Query: 241 TGKTLKVLIQLYSTFSSEFVSVLLEFLLKAMRSSDLAFPKSSKKSSSSVLDGWMLTVAKI 300
           TGKTLKVLIQLYSTFSSEFVSVLLEFLLKAMRSSDLAFPK+SKKSSSSVLDGW LTVAKI
Sbjct: 241 TGKTLKVLIQLYSTFSSEFVSVLLEFLLKAMRSSDLAFPKNSKKSSSSVLDGWKLTVAKI 300

Query: 301 SNKEPELLLDLLEAVLEKIKTQVALEYESQYFLPSDYKMESCHQVAQLSSLFAWLVDKLN 360
           SNKEPELLLDLLEAVLEKIKTQVALEYESQYFLPSD+KMESC QVAQLSSLFAWLVDKLN
Sbjct: 301 SNKEPELLLDLLEAVLEKIKTQVALEYESQYFLPSDHKMESC-QVAQLSSLFAWLVDKLN 360

Query: 361 GIKLKRKGEVFSGKHISKGVLMELLRKCLLVSAPWNKRLLDSATNLAQLLDDDSLVKKLN 420
           GIKLKRKGEVFSGKHI KGVLMELLRKCLLVSAPWNKRLLDSATNLAQLLDDDSLVKKLN
Sbjct: 361 GIKLKRKGEVFSGKHIPKGVLMELLRKCLLVSAPWNKRLLDSATNLAQLLDDDSLVKKLN 420

Query: 421 KLSFVNAPISSIPAEENPNQTASENSFNQAESLREASRKLETIKLRKRTNNMASNPVDSN 480
           KL FVNAPISSIP EENPN +ASE+SFNQAESLREASRKLETIKLRKR NN+ASNPVD N
Sbjct: 421 KLFFVNAPISSIPDEENPNHSASEDSFNQAESLREASRKLETIKLRKRKNNLASNPVDGN 480

Query: 481 VGGSSRWTVVSSWTPCPIGMLPRSIGCSGRPSVLDLNPENEPASEELRSKEKCELNNFSH 540
           VGGSSRWTVVSSWTPCPIGMLPRSIGCSGRPSVLDLNPENEP SEELRSKEKCELNN S 
Sbjct: 481 VGGSSRWTVVSSWTPCPIGMLPRSIGCSGRPSVLDLNPENEPTSEELRSKEKCELNNSSQ 540

Query: 541 KREASSDIQHLDNSIVKKMKETNDKSQFETEDVESPEGIKGRLLIGGIWKNVGEEELSAI 600
           KREASSDI HLDNSIVKKMK+TNDKSQ ETEDVESPEGIKGRLLIGGIWKNVGEEELSAI
Sbjct: 541 KREASSDIHHLDNSIVKKMKDTNDKSQLETEDVESPEGIKGRLLIGGIWKNVGEEELSAI 600

Query: 601 QSAVRILI 608
           QSAVRILI
Sbjct: 601 QSAVRILI 607

BLAST of Chy5G092220 vs. NCBI nr
Match: XP_008449642.1 (PREDICTED: pre-rRNA-processing protein las1 [Cucumis melo])

HSP 1 Score: 1082 bits (2798), Expect = 0.0
Identity = 569/608 (93.59%), Postives = 585/608 (96.22%), Query Frame = 0

Query: 1   MDSLLGFEEVPGENEEENLSTYSRKLVPWSTWNEWLFVSQSLFSDSPDSVAAALSRISTW 60
           MDSLLGFEEV GENEEENLSTYSRKLVPWSTWNEWLFVSQSLFSDSPD VAAALSRISTW
Sbjct: 1   MDSLLGFEEVLGENEEENLSTYSRKLVPWSTWNEWLFVSQSLFSDSPDFVAAALSRISTW 60

Query: 61  RSRGCLPVVIEVTASIIEIQQKDPYFIKNQSMDASVRGTENQLNLVSLSEEALAMLYCMA 120
           RSRGCLPVV+EVTASIIEIQQKDPYFIKNQS+DASVRGTENQLN+VSLSEEALAMLYCMA
Sbjct: 61  RSRGCLPVVVEVTASIIEIQQKDPYFIKNQSIDASVRGTENQLNIVSLSEEALAMLYCMA 120

Query: 121 IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRLLIDIRHEGSHRELPALQVVRHASAKALC 180
           IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRLLIDIRHEGSHRELPALQVVRHASAKALC
Sbjct: 121 IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRLLIDIRHEGSHRELPALQVVRHASAKALC 180

Query: 181 WLKSYYWEPQEKAIPFQGDISTSIRKEIKSKLRGLAFNLEMKQNPQLESALVKPKRLKTK 240
           WLKSYYWEPQEKAIPFQGDISTSIRKEIKSKLR LAFNLE+KQNPQLESALVKPKRLKTK
Sbjct: 181 WLKSYYWEPQEKAIPFQGDISTSIRKEIKSKLRVLAFNLEVKQNPQLESALVKPKRLKTK 240

Query: 241 TGKTLKVLIQLYSTFSSEFVSVLLEFLLKAMRSSDLAFPKSSKKSSSSVLDGWMLTVAKI 300
           TGKT+KVLIQLYSTF SEFVSVLLEFLLKAMRSSDLAFPK+SKKSSSSVLDGW LTVAKI
Sbjct: 241 TGKTMKVLIQLYSTFPSEFVSVLLEFLLKAMRSSDLAFPKNSKKSSSSVLDGWKLTVAKI 300

Query: 301 SNKEPELLLDLLEAVLEKIKTQVALEYESQYFLPSDYKMESCHQVAQLSSLFAWLVDKLN 360
           SNKEPELLLDLLEAVLEKIKTQVALEYESQYF+PSD+K+ESC QV QLSSLFAWLVDKLN
Sbjct: 301 SNKEPELLLDLLEAVLEKIKTQVALEYESQYFMPSDHKVESC-QVVQLSSLFAWLVDKLN 360

Query: 361 GIKLKRKGEVFSGKHISKGVLMELLRKCLLVSAPWNKRLLDSATNLAQLLDDDSLVKKLN 420
           GIKLKRKGEVFSGKHI KG+LMELLRKCLLVSAPWNKRLLDSAT+LAQLLDDDSLVKKLN
Sbjct: 361 GIKLKRKGEVFSGKHIPKGILMELLRKCLLVSAPWNKRLLDSATDLAQLLDDDSLVKKLN 420

Query: 421 KLSFVNAPISSIPAEENPNQTASENSFNQAESLREASRKLETIKLRKRTNNMASNPVDSN 480
           KLSFVN PIS IP  ENPNQTASENSFNQAESLREASRKLE IKLRKR NN+ASNPVD N
Sbjct: 421 KLSFVNVPISPIPDGENPNQTASENSFNQAESLREASRKLEMIKLRKRKNNLASNPVDGN 480

Query: 481 VGGSSRWTVVSSWTPCPIGMLPRSIGCSGRPSVLDLNPENEPASEELRSKEKCELNNFSH 540
           VG SSRWTVVSSWTPCPIGMLPRSIGCSGR SVLDLNPENEPASEE RSK+   LNN SH
Sbjct: 481 VGCSSRWTVVSSWTPCPIGMLPRSIGCSGRLSVLDLNPENEPASEEPRSKDNRALNNSSH 540

Query: 541 KREASSDIQHLDNSIVKKMKETNDKSQFETEDVESPEGIKGRLLIGGIWKNVGEEELSAI 600
           KREASSDIQHLD+SIVKKMKETNDKSQFETEDVESPEGI+GRLLIGGIWK VGEEELSAI
Sbjct: 541 KREASSDIQHLDSSIVKKMKETNDKSQFETEDVESPEGIEGRLLIGGIWKKVGEEELSAI 600

Query: 601 QSAVRILI 608
           QS+VRILI
Sbjct: 601 QSSVRILI 607

BLAST of Chy5G092220 vs. NCBI nr
Match: KAA0061761.1 (pre-rRNA-processing protein las1 [Cucumis melo var. makuwa])

HSP 1 Score: 1075 bits (2781), Expect = 0.0
Identity = 572/631 (90.65%), Postives = 587/631 (93.03%), Query Frame = 0

Query: 1   MDSLLGFEEVPGENEEENLSTYSRKLVPWSTWNEWLFVSQSLFSDSPDSVAAALSR---- 60
           MDSLLGFEEV GENEEENLSTYSRKLVPWSTWNEWLFVSQSLFSDSPDSVAAALSR    
Sbjct: 1   MDSLLGFEEVLGENEEENLSTYSRKLVPWSTWNEWLFVSQSLFSDSPDSVAAALSRVSLL 60

Query: 61  -------------------ISTWRSRGCLPVVIEVTASIIEIQQKDPYFIKNQSMDASVR 120
                              ISTWRSRGCLPVV+EVTASIIEIQQKDPYFIKNQS+DASVR
Sbjct: 61  ISAACLCSVVLTFEVALGPISTWRSRGCLPVVVEVTASIIEIQQKDPYFIKNQSIDASVR 120

Query: 121 GTENQLNLVSLSEEALAMLYCMAIMRLVNGVVEKTRKKTEVSIAVAADAIGLPRLLIDIR 180
           GTENQLN+VSLSEEALAMLYCMAIMRLVNGVVEKTRKKTEVSIAVAADAIGLPRLLIDIR
Sbjct: 121 GTENQLNIVSLSEEALAMLYCMAIMRLVNGVVEKTRKKTEVSIAVAADAIGLPRLLIDIR 180

Query: 181 HEGSHRELPALQVVRHASAKALCWLKSYYWEPQEKAIPFQGDISTSIRKEIKSKLRGLAF 240
           HEGSHRELPALQVVRHASAKALCWLKSYYWEPQEKAIPFQGDISTSIRKEIKSKLR LAF
Sbjct: 181 HEGSHRELPALQVVRHASAKALCWLKSYYWEPQEKAIPFQGDISTSIRKEIKSKLRVLAF 240

Query: 241 NLEMKQNPQLESALVKPKRLKTKTGKTLKVLIQLYSTFSSEFVSVLLEFLLKAMRSSDLA 300
           NLE+KQNPQLESALVKPKRLKTKTGK +KVLIQLYSTFSSEFVSVLLEFLLKAMRSSDLA
Sbjct: 241 NLEVKQNPQLESALVKPKRLKTKTGKIMKVLIQLYSTFSSEFVSVLLEFLLKAMRSSDLA 300

Query: 301 FPKSSKKSSSSVLDGWMLTVAKISNKEPELLLDLLEAVLEKIKTQVALEYESQYFLPSDY 360
           FPK+SKKSSSSVLDGW LTVAKISNKEPELLLDLLEAVLEKIKTQVALEYESQYF+PSD+
Sbjct: 301 FPKNSKKSSSSVLDGWKLTVAKISNKEPELLLDLLEAVLEKIKTQVALEYESQYFMPSDH 360

Query: 361 KMESCHQVAQLSSLFAWLVDKLNGIKLKRKGEVFSGKHISKGVLMELLRKCLLVSAPWNK 420
           K+ESC QV QLSSLFAWLVDKLNGIKLKRKGEVFSGKHI KG+LMELLRKCLLVSAPWNK
Sbjct: 361 KVESC-QVVQLSSLFAWLVDKLNGIKLKRKGEVFSGKHIPKGILMELLRKCLLVSAPWNK 420

Query: 421 RLLDSATNLAQLLDDDSLVKKLNKLSFVNAPISSIPAEENPNQTASENSFNQAESLREAS 480
           RLLDSAT+LAQLLDDDSLVKKLNKLSFVN PIS IP  ENPNQTASENSFNQAESLREAS
Sbjct: 421 RLLDSATDLAQLLDDDSLVKKLNKLSFVNVPISPIPDGENPNQTASENSFNQAESLREAS 480

Query: 481 RKLETIKLRKRTNNMASNPVDSNVGGSSRWTVVSSWTPCPIGMLPRSIGCSGRPSVLDLN 540
           RKLE IKLRKR NN+ASNPVD NVGGSSRWTVVSSWTPCPIGMLPRSIGCSGR SVLDLN
Sbjct: 481 RKLEMIKLRKRKNNLASNPVDGNVGGSSRWTVVSSWTPCPIGMLPRSIGCSGRLSVLDLN 540

Query: 541 PENEPASEELRSKEKCELNNFSHKREASSDIQHLDNSIVKKMKETNDKSQFETEDVESPE 600
           PENEPASEE RSKE   LNN SHKREASSDIQHLD+SIVKKMKETNDKSQFETEDVESPE
Sbjct: 541 PENEPASEEPRSKENRALNNSSHKREASSDIQHLDSSIVKKMKETNDKSQFETEDVESPE 600

Query: 601 GIKGRLLIGGIWKNVGEEELSAIQSAVRILI 608
           GI+GRLLIGGIWK VGEEELSAIQS+VRILI
Sbjct: 601 GIEGRLLIGGIWKKVGEEELSAIQSSVRILI 630

BLAST of Chy5G092220 vs. NCBI nr
Match: TYJ96103.1 (pre-rRNA-processing protein las1 [Cucumis melo var. makuwa])

HSP 1 Score: 1069 bits (2764), Expect = 0.0
Identity = 569/631 (90.17%), Postives = 585/631 (92.71%), Query Frame = 0

Query: 1   MDSLLGFEEVPGENEEENLSTYSRKLVPWSTWNEWLFVSQSLFSDSPDSVAAALSR---- 60
           MDSLLGFEEV GENEEENLSTYSRKLVPWSTWNEWLFVSQSLFSDSPD VAAALSR    
Sbjct: 1   MDSLLGFEEVLGENEEENLSTYSRKLVPWSTWNEWLFVSQSLFSDSPDFVAAALSRVSLL 60

Query: 61  -------------------ISTWRSRGCLPVVIEVTASIIEIQQKDPYFIKNQSMDASVR 120
                              ISTWRSRGCLPVV+EVTASIIEIQQKDPYFIKNQS+DASVR
Sbjct: 61  ISAACLCSVVLTFEVALGPISTWRSRGCLPVVVEVTASIIEIQQKDPYFIKNQSIDASVR 120

Query: 121 GTENQLNLVSLSEEALAMLYCMAIMRLVNGVVEKTRKKTEVSIAVAADAIGLPRLLIDIR 180
           GTENQLN+VSLSEEALAMLYCMAIMRLVNGVVEKTRKKTEVSIAVAADAIGLPRLLIDIR
Sbjct: 121 GTENQLNIVSLSEEALAMLYCMAIMRLVNGVVEKTRKKTEVSIAVAADAIGLPRLLIDIR 180

Query: 181 HEGSHRELPALQVVRHASAKALCWLKSYYWEPQEKAIPFQGDISTSIRKEIKSKLRGLAF 240
           HEGSHRELPALQVVRHASAKALCWLKSYYWEPQEKAIPFQGDISTSIRKEIKSKLR LAF
Sbjct: 181 HEGSHRELPALQVVRHASAKALCWLKSYYWEPQEKAIPFQGDISTSIRKEIKSKLRVLAF 240

Query: 241 NLEMKQNPQLESALVKPKRLKTKTGKTLKVLIQLYSTFSSEFVSVLLEFLLKAMRSSDLA 300
           NLE+KQNPQLESALVKPKRLKTKTGKT+KVLIQLYSTF SEFVSVLLEFLLKAMRSSDLA
Sbjct: 241 NLEVKQNPQLESALVKPKRLKTKTGKTMKVLIQLYSTFPSEFVSVLLEFLLKAMRSSDLA 300

Query: 301 FPKSSKKSSSSVLDGWMLTVAKISNKEPELLLDLLEAVLEKIKTQVALEYESQYFLPSDY 360
           FPK+SKKSSSSVLDGW LTVAKISNKEPELLLDLLEAVLEKIKTQVALEYESQYF+PSD+
Sbjct: 301 FPKNSKKSSSSVLDGWKLTVAKISNKEPELLLDLLEAVLEKIKTQVALEYESQYFMPSDH 360

Query: 361 KMESCHQVAQLSSLFAWLVDKLNGIKLKRKGEVFSGKHISKGVLMELLRKCLLVSAPWNK 420
           K+ESC QV QLSSLFAWLVDKLNGIKLKRKGEVFSGKHI KG+LMELLRKCLLVSAPWNK
Sbjct: 361 KVESC-QVVQLSSLFAWLVDKLNGIKLKRKGEVFSGKHIPKGILMELLRKCLLVSAPWNK 420

Query: 421 RLLDSATNLAQLLDDDSLVKKLNKLSFVNAPISSIPAEENPNQTASENSFNQAESLREAS 480
           RLLDSAT+LAQLLDDDSLVKKLNKLSFVN PIS IP  ENPNQTASENSFNQAESLREAS
Sbjct: 421 RLLDSATDLAQLLDDDSLVKKLNKLSFVNVPISPIPDGENPNQTASENSFNQAESLREAS 480

Query: 481 RKLETIKLRKRTNNMASNPVDSNVGGSSRWTVVSSWTPCPIGMLPRSIGCSGRPSVLDLN 540
           RKLE IKLRKR NN+ASNPVD NVG SSRWTVVSSWTPCPIGMLPRSIGCSGR SVLDLN
Sbjct: 481 RKLEMIKLRKRKNNLASNPVDGNVGCSSRWTVVSSWTPCPIGMLPRSIGCSGRLSVLDLN 540

Query: 541 PENEPASEELRSKEKCELNNFSHKREASSDIQHLDNSIVKKMKETNDKSQFETEDVESPE 600
           PENEPASEE RSK+   LNN SHKREASSDIQHLD+SIVKKMKETNDKSQFETEDVESPE
Sbjct: 541 PENEPASEEPRSKDNRALNNSSHKREASSDIQHLDSSIVKKMKETNDKSQFETEDVESPE 600

Query: 601 GIKGRLLIGGIWKNVGEEELSAIQSAVRILI 608
           GI+GRLLIGGIWK VGEEELSAIQS+VRILI
Sbjct: 601 GIEGRLLIGGIWKKVGEEELSAIQSSVRILI 630

BLAST of Chy5G092220 vs. NCBI nr
Match: XP_038902156.1 (pre-rRNA-processing protein las1 isoform X1 [Benincasa hispida] >XP_038902157.1 pre-rRNA-processing protein las1 isoform X1 [Benincasa hispida])

HSP 1 Score: 996 bits (2575), Expect = 0.0
Identity = 525/609 (86.21%), Postives = 567/609 (93.10%), Query Frame = 0

Query: 1   MDSLLGFEEVPGENEEENLSTYSRKLVPWSTWNEWLFVSQSLFSDSPDSVAAALSRISTW 60
           M+SLLGF+EV  ENEEEN STYSRKLVPWSTW+EWLFV QSLFSDSPDSVAAALSRISTW
Sbjct: 1   MESLLGFQEVLVENEEENSSTYSRKLVPWSTWSEWLFVGQSLFSDSPDSVAAALSRISTW 60

Query: 61  RSRGCLPVVIEVTASIIEIQQKDPYFIKNQSMDASVRGTENQLNLVSLSEEALAMLYCMA 120
           RSRGCLPVV+EVTASI+EIQQKDPYFIKNQSM+ASV GTENQL+ VSLSEEALAMLYCMA
Sbjct: 61  RSRGCLPVVVEVTASIVEIQQKDPYFIKNQSMNASVCGTENQLSNVSLSEEALAMLYCMA 120

Query: 121 IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRLLIDIRHEGSHRELPALQVVRHASAKALC 180
           IMRLVNGVVEKTRKKTEVSIAVAADAIGLPR+LIDIRHEGSHRELPALQVVR AS KAL 
Sbjct: 121 IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRVLIDIRHEGSHRELPALQVVRSASIKALR 180

Query: 181 WLKSYYWEPQEKAIPFQGDISTSIRKEIKSKLRGLAFNLEMKQNPQLESALVKPKRLKTK 240
           WLKSYYW+PQEKAIPFQGDIST+IRKEIKSKLR LAFNL++KQNP+LESALVKPKRLKTK
Sbjct: 181 WLKSYYWQPQEKAIPFQGDISTTIRKEIKSKLRDLAFNLKVKQNPELESALVKPKRLKTK 240

Query: 241 TGKTLKVLIQLYSTFSSEFVSVLLEFLLKAMRSSDLAFPKSSKKSSSSVLDGWMLTVAKI 300
           +GKTLKVLIQLYSTFSSEFVSVLLEFLLKAMRSSDLAFPKSSKKSSSSVLDGW L ++KI
Sbjct: 241 SGKTLKVLIQLYSTFSSEFVSVLLEFLLKAMRSSDLAFPKSSKKSSSSVLDGWKLAISKI 300

Query: 301 SNKEPELLLDLLEAVLEKIKTQVALEYESQYFLPSDYKMESCHQVAQLSSLFAWLVDKLN 360
           SNKEPELLLDLLEA+LEKIKTQVALEYES+YF+ SD+KMESC QVAQLSSLFAWLV+KLN
Sbjct: 301 SNKEPELLLDLLEAILEKIKTQVALEYESKYFMSSDHKMESC-QVAQLSSLFAWLVNKLN 360

Query: 361 GIKLKRKGEVFSGKHISKGVLMELLRKCLLVSAPWNKRLLDSATNLAQLLDDDSLVKKLN 420
           GIK KRKGEV SGKHI K +L+ELLRKCLLVSAP NKRL+DSAT+LAQLLDDDSLVKKLN
Sbjct: 361 GIKPKRKGEVISGKHIPKSILVELLRKCLLVSAPCNKRLMDSATDLAQLLDDDSLVKKLN 420

Query: 421 KLSFVNAPISSIPAEENPNQTASENSFNQAESLREASRKLETIKLRKRTNNMASNPVDSN 480
           KLSF+N  IS IP EENP QT  ENSFNQ ESL EAS+KLE IKL KR NN+A+NPVD N
Sbjct: 421 KLSFLNELISPIPDEENPIQTMPENSFNQVESLHEASQKLEMIKLHKRKNNLAANPVDGN 480

Query: 481 VGGSSRWTVVSSWTPCPIGMLPRSIGCSGRPSVLDLNPENEPASEELRSKEKCELNNFSH 540
           VG SSRWTVVSSWTPCPIGMLPRSIGCSGRP+VL+LNPE+EP+SEEL++KE CEL+N S+
Sbjct: 481 VGSSSRWTVVSSWTPCPIGMLPRSIGCSGRPAVLNLNPESEPSSEELKTKENCELDNCSN 540

Query: 541 K-REASSDIQHLDNSIVKKMKETNDKSQFETEDVESPEGIKGRLLIGGIWKNVGEEELSA 600
           K REASSDI+HLD S VKKMKETN+  Q ET++VESPEG+KGRLLIGGIWK VGEEELSA
Sbjct: 541 KKREASSDIRHLDRSTVKKMKETNENVQSETQNVESPEGVKGRLLIGGIWKKVGEEELSA 600

Query: 601 IQSAVRILI 608
           IQSAVRILI
Sbjct: 601 IQSAVRILI 608

BLAST of Chy5G092220 vs. TAIR 10
Match: AT5G12220.1 (las1-like family protein )

HSP 1 Score: 381.3 bits (978), Expect = 1.4e-105
Identity = 262/643 (40.75%), Postives = 367/643 (57.08%), Query Frame = 0

Query: 1   MDSLLG----FEEVPGENEEENLSTYSRKLVPWSTWNEWLFVSQSLFSDSPDSVAAALSR 60
           M++L G     E   GE +++  S    K VPW +W EW  V +SLFS SPD +A+AL R
Sbjct: 1   MEALAGIEADIESYFGEQDQQKSSPDGCKQVPWLSWEEWDSVRESLFSSSPDRIASALER 60

Query: 61  ISTWRSRGCLPVVIEVTASIIEIQQKDPYFIK-NQSMDASVRGTENQLNLVSLSEEALAM 120
           ++TWRSRG LP  ++VT S+IEIQ KD +  +  QS DA              SE  L M
Sbjct: 61  VATWRSRGSLPAPVDVTCSLIEIQLKDGFIPREKQSADA------------LYSEHLLQM 120

Query: 121 LYCMAIMRLVNGVVEKTRKKTEVSIAVAADAIGLPRLLIDIRHEGSHRELPALQVVRHAS 180
           LYCM I+RLVN V+EKTR++ +VSIA AA AIG+PR LID+RHEGSHRELPAL V+R A+
Sbjct: 121 LYCMGILRLVNCVIEKTRRREDVSIADAARAIGIPRKLIDLRHEGSHRELPALSVLRDAA 180

Query: 181 AKALCWLKSYYWEPQEKAIPFQGDISTSIRKEIKSKLRGLAFNLEMKQNPQLESALVKPK 240
            +AL WLKSYYWE Q+  IP + D + SIR+E+KSKLR LAF+L++K+NPQ +S LVK K
Sbjct: 181 DEALEWLKSYYWEQQKYQIPLKRDGTASIRREVKSKLRKLAFSLQLKRNPQFDSPLVKEK 240

Query: 241 RLKTKTGKTLKVLIQLYSTFSSEFVSVLLEFLLKAMRSSDLAFPKSSKKSSSSV-LDGWM 300
               +  K +  L++LY +FS+E  SVLLEFLLKA+ SS     ++       V LD W 
Sbjct: 241 CSNKRIRKIVSSLVELYPSFSAEISSVLLEFLLKALDSSKSTDLQNKAGQDFRVFLDEWK 300

Query: 301 LTVAKISNKEPELLLDLLEAVLEKIKTQVALEYESQYFLPSDYKMESCHQVAQLSSLFAW 360
             + ++SN+EPELLL LL+ VL+ I+      +E      ++  +E   QV Q+  LFAW
Sbjct: 301 PVIMELSNREPELLLTLLKEVLDMIQNNERRRHE------TEKPVEEVSQVEQVPFLFAW 360

Query: 361 LVDKLNGIKLKRKGEVFSGKHISKGVLMELLRKCLLVSAPWNKRLLDSATNLAQLLDDDS 420
           LV  LNG K  ++      K  S   LMEL+R+CL++ A   + +L SA  LA+++    
Sbjct: 361 LVSLLNGSKHFQRNSSLEVKPPSI-FLMELIRRCLVLGALGYELVLKSAFLLAEIVGGRV 420

Query: 421 LVKKLNKLSFVNAPISSIPAEENPN-QTASENSFNQAESLREASRKLETIKLRKRTNNMA 480
           L +KL KL  ++   + +P +++    TAS     Q ++L  A ++LE +KL+       
Sbjct: 421 LKEKLIKLPLMHKSSAIVPLKQSSTLVTASTTLLEQEKNLGNAGKRLEFVKLQL----SK 480

Query: 481 SNPVDSNVGGSSRWTVVSSWTPCPIGMLPRSIGCSGRPSVLDLNPENEPASEELRSKEKC 540
              +D++  G +RW    +W+PCPIGMLPR IG SGR  +LD        ++  +S  K 
Sbjct: 481 KKGIDTDKTG-NRWRKAKTWSPCPIGMLPRIIGSSGRLPLLD--------NQNAKSISKQ 540

Query: 541 ELNNFSHKREASSDIQHLDNSIVKKMK------ETND---------------KSQFETED 600
              N + KR A  +   L+ S  K+ +      E+ND                +  ETE 
Sbjct: 541 AQGNNNAKRGAECNRNQLEKSPCKRARKSAGDSESNDVTLESYEEEAEMEIEHAYEETET 600

Query: 601 V-------ESPEGIKGRLLIGGIWKNVGEEELSAIQSAVRILI 609
           V          E  +  L+I G WK V + ELS + S+V I +
Sbjct: 601 VAEENLMWNDEEESRSCLMIDGEWKRVNDGELSGMASSVTICV 611

BLAST of Chy5G092220 vs. TAIR 10
Match: AT5G12220.2 (las1-like family protein )

HSP 1 Score: 354.8 bits (909), Expect = 1.4e-97
Identity = 254/643 (39.50%), Postives = 357/643 (55.52%), Query Frame = 0

Query: 1   MDSLLG----FEEVPGENEEENLSTYSRKLVPWSTWNEWLFVSQSLFSDSPDSVAAALSR 60
           M++L G     E   GE +++  S    K VPW +W EW  V +SLFS SPD +A+AL R
Sbjct: 1   MEALAGIEADIESYFGEQDQQKSSPDGCKQVPWLSWEEWDSVRESLFSSSPDRIASALER 60

Query: 61  ISTWRSRGCLPVVIEVTASIIEIQQKDPYFIK-NQSMDASVRGTENQLNLVSLSEEALAM 120
           ++TWRSRG LP  ++VT S+IEIQ KD +  +  QS DA              SE  L M
Sbjct: 61  VATWRSRGSLPAPVDVTCSLIEIQLKDGFIPREKQSADA------------LYSEHLLQM 120

Query: 121 LYCMAIMRLVNGVVEKTRKKTEVSIAVAADAIGLPRLLIDIRHEGSHRELPALQVVRHAS 180
           LYCM I+RLVN V+EKTR++ +VSIA AA AI           EGSHRELPAL V+R A+
Sbjct: 121 LYCMGILRLVNCVIEKTRRREDVSIADAARAI-----------EGSHRELPALSVLRDAA 180

Query: 181 AKALCWLKSYYWEPQEKAIPFQGDISTSIRKEIKSKLRGLAFNLEMKQNPQLESALVKPK 240
            +AL WLKSYYWE Q+  IP + D + SIR+E+KSKLR LAF+L++K+NPQ +S LVK K
Sbjct: 181 DEALEWLKSYYWEQQKYQIPLKRDGTASIRREVKSKLRKLAFSLQLKRNPQFDSPLVKEK 240

Query: 241 RLKTKTGKTLKVLIQLYSTFSSEFVSVLLEFLLKAMRSSDLAFPKSSKKSSSSV-LDGWM 300
               +  K +  L++LY +FS+E  SVLLEFLLKA+ SS     ++       V LD W 
Sbjct: 241 CSNKRIRKIVSSLVELYPSFSAEISSVLLEFLLKALDSSKSTDLQNKAGQDFRVFLDEWK 300

Query: 301 LTVAKISNKEPELLLDLLEAVLEKIKTQVALEYESQYFLPSDYKMESCHQVAQLSSLFAW 360
             + ++SN+EPELLL LL+ VL+ I+      +E      ++  +E   QV Q+  LFAW
Sbjct: 301 PVIMELSNREPELLLTLLKEVLDMIQNNERRRHE------TEKPVEEVSQVEQVPFLFAW 360

Query: 361 LVDKLNGIKLKRKGEVFSGKHISKGVLMELLRKCLLVSAPWNKRLLDSATNLAQLLDDDS 420
           LV  LNG K  ++      K  S   LMEL+R+CL++ A   + +L SA  LA+++    
Sbjct: 361 LVSLLNGSKHFQRNSSLEVKPPSI-FLMELIRRCLVLGALGYELVLKSAFLLAEIVGGRV 420

Query: 421 LVKKLNKLSFVNAPISSIPAEENPN-QTASENSFNQAESLREASRKLETIKLRKRTNNMA 480
           L +KL KL  ++   + +P +++    TAS     Q ++L  A ++LE +KL+       
Sbjct: 421 LKEKLIKLPLMHKSSAIVPLKQSSTLVTASTTLLEQEKNLGNAGKRLEFVKLQL----SK 480

Query: 481 SNPVDSNVGGSSRWTVVSSWTPCPIGMLPRSIGCSGRPSVLDLNPENEPASEELRSKEKC 540
              +D++  G +RW    +W+PCPIGMLPR IG SGR  +LD        ++  +S  K 
Sbjct: 481 KKGIDTDKTG-NRWRKAKTWSPCPIGMLPRIIGSSGRLPLLD--------NQNAKSISKQ 540

Query: 541 ELNNFSHKREASSDIQHLDNSIVKKMK------ETND---------------KSQFETED 600
              N + KR A  +   L+ S  K+ +      E+ND                +  ETE 
Sbjct: 541 AQGNNNAKRGAECNRNQLEKSPCKRARKSAGDSESNDVTLESYEEEAEMEIEHAYEETET 600

Query: 601 V-------ESPEGIKGRLLIGGIWKNVGEEELSAIQSAVRILI 609
           V          E  +  L+I G WK V + ELS + S+V I +
Sbjct: 601 VAEENLMWNDEEESRSCLMIDGEWKRVNDGELSGMASSVTICV 600

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9Y4W23.1e-1230.49Ribosomal biogenesis protein LAS1L OS=Homo sapiens OX=9606 GN=LAS1L PE=1 SV=2[more]
A2BE282.0e-1128.33Ribosomal biogenesis protein LAS1L OS=Mus musculus OX=10090 GN=Las1l PE=1 SV=1[more]
P361463.7e-1028.65Protein LAS1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 ... [more]
O429363.5e-0820.31Pre-rRNA-processing protein las1 OS=Schizosaccharomyces pombe (strain 972 / ATCC... [more]
Match NameE-valueIdentityDescription
A0A0A0KI890.0e+0096.38Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G430640 PE=4 SV=1[more]
A0A1S3BNF10.0e+0093.59pre-rRNA-processing protein las1 OS=Cucumis melo OX=3656 GN=LOC103491463 PE=4 SV... [more]
A0A5A7V7Q20.0e+0090.65Pre-rRNA-processing protein las1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A5D3B9Y35.9e-30990.17Pre-rRNA-processing protein las1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A6J1H0C52.0e-27283.06uncharacterized protein LOC111459262 OS=Cucurbita moschata OX=3662 GN=LOC1114592... [more]
Match NameE-valueIdentityDescription
XP_004140277.10.096.38pre-rRNA-processing protein las1 [Cucumis sativus] >XP_031744103.1 pre-rRNA-proc... [more]
XP_008449642.10.093.59PREDICTED: pre-rRNA-processing protein las1 [Cucumis melo][more]
KAA0061761.10.090.65pre-rRNA-processing protein las1 [Cucumis melo var. makuwa][more]
TYJ96103.10.090.17pre-rRNA-processing protein las1 [Cucumis melo var. makuwa][more]
XP_038902156.10.086.21pre-rRNA-processing protein las1 isoform X1 [Benincasa hispida] >XP_038902157.1 ... [more]
Match NameE-valueIdentityDescription
AT5G12220.11.4e-10540.75las1-like family protein [more]
AT5G12220.21.4e-9739.50las1-like family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007174Las1PFAMPF04031Las1coord: 27..188
e-value: 2.1E-40
score: 138.3
IPR007174Las1PANTHERPTHR15002UNCHARACTERIZEDcoord: 19..606

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy5G092220.1Chy5G092220.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0006364 rRNA processing
cellular_component GO:0090730 Las1 complex
molecular_function GO:0004519 endonuclease activity