Chy5G091750 (gene) Cucumber (hystrix) v1

Overview


Name	Chy5G091750
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	tRNA-5-taurinomethyluridine 2-sulfurtransferase
Location	chrH05: 999070 .. 1003580 (-)
RNA-Seq Expression	Chy5G091750
Synteny	Chy5G091750

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGCTGAGACTGAGAGTGAATCCCGCCGTTTGGTGCTCTGTTCCGATTTCCCATTTCACCTCTCTTCCCCTTTCCCCTTCAATTCTCAGACCTCTTCTCCGCCGCCACAACCCTCGTCTCTTCCTCACCCGATTCTCCGCTTCCTCTGCCGTGCATAATACCTCCCTATCTTCTTCACCCAAACCCCTCTCCGATGCCGACTTCCTCCCTTTCCTCTCATGTTCAATGCCTACTTATCCTCTCAAAGTCGCCGTTCTTCTCAGCGGCGGCGTCGATAGTAGCGTCGCACTCCGCCTTCTCCACGCTGCTGGCCACTCCTGCACTGCCTTCTACCTCAAAATCTGGTTCCAGGTGTGTTATGTTATATGGTTCTTTGTGTTTTCTTGCAAATTATGGTAACTGGTAAAGTGAATGCTGGCGCAGTTGTGAGTAATCAAATGGGCATTTCATCTCGAAGGTAATAACCTTTTTGTGTGAACGGACTTTAATGGGTTCTGTTTTCGTAGGAAGACTTCGAGAATTTTTGGTCAGAATGTCCATGGGACGATGATTTGAAGTATGCTAAAGCTGTTTGTGATCAGGTTTGTTACTATTTTAGCCTCTAAGTCCATCTAGACTTACAAAGGTTAATTTTTCCAACGAATTTCAAATGAAATAAAGGATTTGTTGGGAAAGTTGAATATAATTCAAGATACCCACAAGTAATGTCTTTCTTCTGTAGTTTGTGCTCAAGAGAGATCTTTGAGCTGAAAATGTTATGTAAATTGGTTTTCAGGTTGATGTACCTTTGGAAGTAGTTCATTTGACAGAGGAATATTGGAGGAACGTGGTAAGTGCATTTACAACTACAATTTTATCCTATAACTTTGTTGGATATCTTAATGATATATTAGTACATCCAATCAATTTACTTGTAGCTGTTCCGGCTGACCATCTTCCTCATTCATTCTTTTTCTAGCATGACAAACTGGTATTGCGTTCTCATTTATGTCGACTGAATATTGTTTATTTCATGCTTTTAGGTTTCTTATATAATTGAAGAATATCGGTGTGGCCGTACCCCAAACCCCGATGTTCTTTGCAACACAAGAATAAAGTTTGGTATGTTCATTAGATGGACATGAAAATTTGGTTATATTGTCAAGCTTTCTATTTATGTTTATGATTTCTTTTATATTAATCTGATATATGGCAAGCCCTGCTTTTTCCAGGAGCATTCATGGATGCAATAAGCAATATGGAGTTTGATTATGTTGCTTCAGGGCATTATGCGAATGTTATTCACCCATCTGCTGATCAAATGGATAAACCTTCTGTTTTAGAACTGTCCAAGGACATGGTATCTGGGAACGATTGCATGGACCTTTCTTTTAATCTAACCTGTCCAATAGGGAGTATCAGTTATTCCTTGCTTAACTTTATTATTTTGTGGTAGGGAAAGGGATCATAGTGAGGTTTGGTCTTTCATTAGATTTCATGTGTCTCTTTGGGCTTCAATTTCGATCTCCTTTTGTAATTATTCCATAGGTGATATCCTTCTCAGTTGGTCCCCCCTTCTTTAGTTGGAGTGATTTCTGTGGGCTGGTTTTTTTGTGGCCTTTTAGGTAGGACACTATTTTTCTATTTTCATGACATTTGTCTATATTCTACACTACTCATGGCAGTTACTAATGCCTTTAGTAATTTAACTTGAAGTTTCATGTATAAACTCAGTGGAATAGATATGTCTAATATTGCATTATACGGCCAATATTTTTGCAATCCTTCAGGAAACAACATCATTAATAAGCTTTATGATTTATGTATCGCTTACCTAGTTCAAGGTTTATCATCAAACACATTTATCCCTTCCTTGTTCCTTCTTCCTCCAAAATAAAGATTTCTTATTTTACTTTGAATATTGTTGTTCTTAGGTCAAGGATCAAACGTATTTTCTATCACATCTCTCACAGAATCAACTTAAACGGCTTTTATTCCCTCTTGGTTGTATTCCAAAGGTTTGTTCAAGCTAAATTTGTAGTATTTGTTACGTATTTTTTTTAGGTATCTCATATGTTTCAGACTTCAGGGTGTATTCCGAGAGGTTCAAATTCAACTATTGTAAATTTGTATGATTTGGTACTGGCCTTCTAAGCTTGTACAGACATAAACACCTAGTGGCTAACATTGGTTGCATTCAGTTGAGAAATTTATGTATTTAATTAAAGTTAAAAATTGTATTACAGACTTTGGCACGCCAGTTGAAGTCGATAACATTTTAGTGATTTGTGGATGTTATATTGCAGTTTGAACCTATTAAGTTTTTACTATTCTCGTGTGCAAAACTTATATAAGTGCCTCAATTTATTAGTTAATATCATGATAACTCCTATCCCAACTAGCTACATTCTTTCAACTTCTGCTCTATCTTTTCTGAAGGAAAAAGGAATACTTGTGTGCTTGTTATCTTGTTAATATGTTTTTCTAGGGTTGTGACTTCAGCAATATTTTTCTTCTCTCAGCCTTATATTCCCTCAACAATCATCTGCAGGATGATGTTCGCAAGCTTGCTGCAAAATTCAACCTACCAAACAAAGACAGAAAGGATTCACAAGGAATATGTTTCCTTGGGAAGGTACTTTGGATGTGGTGATTCATACATTTCTTTTGTTTGTCTTTTTTTTTTCGGGAGGCTAATGCCTTTCTCAAAATTCTTTGTTCAATGCTTATCTATTTCTAATATTTTGGTGTTGCAATTTTATATTTTAGAATATTTTTTACTACTTTTTAGAACTATTTATAGTAATAAAGGCTGGCTTAAAGGATTTAGTACAAGATTTGCCAACACTTGTTGAAATTTCCTGATGGCTATACTTTCCCTGAGCAAGTGTTTTATGTCCTTATTCTTCCTGACACCAACTATTTTCATTTATCCTGTTTCTGAAGATGGGTCCTGTAAGTTATCTTGTGGTAGAATTCATGTAGAACCTGTTTCTTTGATGTGTAGATAAAATTTAGTGAATTTGTTGCAAGACATATTGGGGAGGATGAAGGTGTGATATTGGAGGCAGAGTCAGGAGATTTTCTTGGCAAACATCGAGGATTTTGGTTCTACACGATTGGCCAACGCCAAGGTCTAAGACTTCCTGGAGGACCTTGGTATGCCTTTTCAGTTTCTTTTCTTTCAGAATAAATTTGATGATCTCTAGCTGTTGTATGACCATTTTACCAAAACTTCAAGAACATCTTCAACTGAGAGAATTGAGCATTCCGATGTGCCAGTTTAGGTTTTAGTGACGTGATTACATTGAGATTATTTCCAATCTGGTGCTTCACAAGCACACATTCTTTACTCACGTCTCTTGCTGAGCTATATTACAATGACGATTTCAGCTTTTTTGCCTTCATCTAATGCAGGTATGTCGTGGAAAAAGATATAAAGAACAATGTAGTATTTGTGTCGAGGAACTATTTCTCATTTGACAAAAGGAGGCGGGTGTTTCGTGTTGGATCTCTAAAATGGCTTAGTGGTTTGCCTCCAGACCAATTTACTGGGCTTCAATGTAAGGTATAGTTTCAGCTTCCATCTCCCATATTTCTTGTTAGTTTCACTACTTCAAGTTGGCCTGGCCGTAACGATTTAACATAAAAGAAATTTTTCTTAAACTTTTTTACAAACGTTTCCGGAGAACCCATCAGTTTATTCCTGCTAGTTTGTGTTGGAAGTTTGACAAGCATGCTTGGTAATATTGATAATCCGGAAATGTTGACTATTACATGAAAAAAAAAATCTTCCATGATTATTTAATAGAACAAGCATCTCGACATCATCTGTTTTTCTTTCAAACAAAGGATTTAAGTTTAGAATTCAATTCTATCTGTTAGGGGACTGTGGCCTTTCTCTTTGAGAAGGGAAAATGTAGTAAGATTAAGTAGATGATCTAGAAATGTTATGGGGAGGCTATGTCCGTTATATTCCGCCCATTTGCTTTGAGATTGTGTTATTGGACTCTAGTTCTCATCATGTCATTTCGACTTCATTGTTTCATAATTTTATACAATTCAATTTCTAGGTCCGACACGGCCCGAGTTTCTATAGTTGCAATTTGACAATGGAACACAATGAGGATGGACATGAAGACGTTGCAGTTGTCCACCTAAGCGAAGACGACCAAGGCCTCGCAGCAGGCCAGTTTGCAGCCTTTTACAAGGGAAGGAGCTGCATTGGGTCAGGTGTGATTTTGGAATCATGGGATGACCAAGGGTTTCCTGTATGTCAAAAGGCTATTGAAAATGCAAAAATGGAAGATAAATCGAAACTTGGCAAACCAATAAAGATAAAAGTGAAACCAGAGAGTGGTACGAAAGAATCTAAAGCCAAAGACGATACAAAAATACAAGACGAGTTAAGGAATGAAGAAGCCACTGTGGTTGAGCAAATAAGAGAAGTATCTCCAGATGAAGCAATGGCCACATCCTCAATGAAATGGCTTGGAAGTCTTAAGAAGAAGTTGCTGCAGATATTTTAG

mRNA sequence

ATGATGCTGAGACTGAGAGTGAATCCCGCCGTTTGGTGCTCTGTTCCGATTTCCCATTTCACCTCTCTTCCCCTTTCCCCTTCAATTCTCAGACCTCTTCTCCGCCGCCACAACCCTCGTCTCTTCCTCACCCGATTCTCCGCTTCCTCTGCCGTGCATAATACCTCCCTATCTTCTTCACCCAAACCCCTCTCCGATGCCGACTTCCTCCCTTTCCTCTCATGTTCAATGCCTACTTATCCTCTCAAAGTCGCCGTTCTTCTCAGCGGCGGCGTCGATAGTAGCGTCGCACTCCGCCTTCTCCACGCTGCTGGCCACTCCTGCACTGCCTTCTACCTCAAAATCTGGTTCCAGGAAGACTTCGAGAATTTTTGGTCAGAATGTCCATGGGACGATGATTTGAAGTATGCTAAAGCTGTTTGTGATCAGGTTGATGTACCTTTGGAAGTAGTTCATTTGACAGAGGAATATTGGAGGAACGTGGTTTCTTATATAATTGAAGAATATCGGTGTGGCCGTACCCCAAACCCCGATGTTCTTTGCAACACAAGAATAAAGTTTGGAGCATTCATGGATGCAATAAGCAATATGGAGTTTGATTATGTTGCTTCAGGGCATTATGCGAATGTTATTCACCCATCTGCTGATCAAATGGATAAACCTTCTGTTTTAGAACTGTCCAAGGACATGGTCAAGGATCAAACGTATTTTCTATCACATCTCTCACAGAATCAACTTAAACGGCTTTTATTCCCTCTTGGTTGTATTCCAAAGGATGATGTTCGCAAGCTTGCTGCAAAATTCAACCTACCAAACAAAGACAGAAAGGATTCACAAGGAATATGTTTCCTTGGGAAGATAAAATTTAGTGAATTTGTTGCAAGACATATTGGGGAGGATGAAGGTGTGATATTGGAGGCAGAGTCAGGAGATTTTCTTGGCAAACATCGAGGATTTTGGTTCTACACGATTGGCCAACGCCAAGGTCTAAGACTTCCTGGAGGACCTTGGTATGTCGTGGAAAAAGATATAAAGAACAATGTAGTATTTGTGTCGAGGAACTATTTCTCATTTGACAAAAGGAGGCGGGTGTTTCGTGTTGGATCTCTAAAATGGCTTAGTGGTTTGCCTCCAGACCAATTTACTGGGCTTCAATGTAAGGTCCGACACGGCCCGAGTTTCTATAGTTGCAATTTGACAATGGAACACAATGAGGATGGACATGAAGACGTTGCAGTTGTCCACCTAAGCGAAGACGACCAAGGCCTCGCAGCAGGCCAGTTTGCAGCCTTTTACAAGGGAAGGAGCTGCATTGGGTCAGGTGTGATTTTGGAATCATGGGATGACCAAGGGTTTCCTGTATGTCAAAAGGCTATTGAAAATGCAAAAATGGAAGATAAATCGAAACTTGGCAAACCAATAAAGATAAAAGTGAAACCAGAGAGTGGTACGAAAGAATCTAAAGCCAAAGACGATACAAAAATACAAGACGAGTTAAGGAATGAAGAAGCCACTGTGGTTGAGCAAATAAGAGAAGTATCTCCAGATGAAGCAATGGCCACATCCTCAATGAAATGGCTTGGAAGTCTTAAGAAGAAGTTGCTGCAGATATTTTAG

Coding sequence (CDS)

ATGATGCTGAGACTGAGAGTGAATCCCGCCGTTTGGTGCTCTGTTCCGATTTCCCATTTCACCTCTCTTCCCCTTTCCCCTTCAATTCTCAGACCTCTTCTCCGCCGCCACAACCCTCGTCTCTTCCTCACCCGATTCTCCGCTTCCTCTGCCGTGCATAATACCTCCCTATCTTCTTCACCCAAACCCCTCTCCGATGCCGACTTCCTCCCTTTCCTCTCATGTTCAATGCCTACTTATCCTCTCAAAGTCGCCGTTCTTCTCAGCGGCGGCGTCGATAGTAGCGTCGCACTCCGCCTTCTCCACGCTGCTGGCCACTCCTGCACTGCCTTCTACCTCAAAATCTGGTTCCAGGAAGACTTCGAGAATTTTTGGTCAGAATGTCCATGGGACGATGATTTGAAGTATGCTAAAGCTGTTTGTGATCAGGTTGATGTACCTTTGGAAGTAGTTCATTTGACAGAGGAATATTGGAGGAACGTGGTTTCTTATATAATTGAAGAATATCGGTGTGGCCGTACCCCAAACCCCGATGTTCTTTGCAACACAAGAATAAAGTTTGGAGCATTCATGGATGCAATAAGCAATATGGAGTTTGATTATGTTGCTTCAGGGCATTATGCGAATGTTATTCACCCATCTGCTGATCAAATGGATAAACCTTCTGTTTTAGAACTGTCCAAGGACATGGTCAAGGATCAAACGTATTTTCTATCACATCTCTCACAGAATCAACTTAAACGGCTTTTATTCCCTCTTGGTTGTATTCCAAAGGATGATGTTCGCAAGCTTGCTGCAAAATTCAACCTACCAAACAAAGACAGAAAGGATTCACAAGGAATATGTTTCCTTGGGAAGATAAAATTTAGTGAATTTGTTGCAAGACATATTGGGGAGGATGAAGGTGTGATATTGGAGGCAGAGTCAGGAGATTTTCTTGGCAAACATCGAGGATTTTGGTTCTACACGATTGGCCAACGCCAAGGTCTAAGACTTCCTGGAGGACCTTGGTATGTCGTGGAAAAAGATATAAAGAACAATGTAGTATTTGTGTCGAGGAACTATTTCTCATTTGACAAAAGGAGGCGGGTGTTTCGTGTTGGATCTCTAAAATGGCTTAGTGGTTTGCCTCCAGACCAATTTACTGGGCTTCAATGTAAGGTCCGACACGGCCCGAGTTTCTATAGTTGCAATTTGACAATGGAACACAATGAGGATGGACATGAAGACGTTGCAGTTGTCCACCTAAGCGAAGACGACCAAGGCCTCGCAGCAGGCCAGTTTGCAGCCTTTTACAAGGGAAGGAGCTGCATTGGGTCAGGTGTGATTTTGGAATCATGGGATGACCAAGGGTTTCCTGTATGTCAAAAGGCTATTGAAAATGCAAAAATGGAAGATAAATCGAAACTTGGCAAACCAATAAAGATAAAAGTGAAACCAGAGAGTGGTACGAAAGAATCTAAAGCCAAAGACGATACAAAAATACAAGACGAGTTAAGGAATGAAGAAGCCACTGTGGTTGAGCAAATAAGAGAAGTATCTCCAGATGAAGCAATGGCCACATCCTCAATGAAATGGCTTGGAAGTCTTAAGAAGAAGTTGCTGCAGATATTTTAG

Protein sequence

MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRRHNPRLFLTRFSASSAVHNTSLSSSPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDDVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGLPPDQFTGLQCKVRHGPSFYSCNLTMEHNEDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAKMEDKSKLGKPIKIKVKPESGTKESKAKDDTKIQDELRNEEATVVEQIREVSPDEAMATSSMKWLGSLKKKLLQIF*

Homology

[Close]

BLAST of Chy5G091750 vs. ExPASy Swiss-Prot
Match: Q0SMH1 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia afzelii (strain PKo) OX=390236 GN=mnmA PE=3 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 3.5e-100
Identity = 169/365 (46.30%), Postives = 249/365 (68.22%), Query Frame = 0


Query: 82  LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 141
           +K+AVLLSGGVDSSVAL R+++    S   +YLKIW +++  ++   CPW +DL Y +AV
Sbjct: 1   MKIAVLLSGGVDSSVALYRIINKGYTSIKCYYLKIWLEDEL-SYIGSCPWQEDLNYVEAV 60

Query: 142 CDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 201
           C + +VP E+++  +EY+  VVSY I+E + G TP+PD+ CN RIKFGAF + I N ++D
Sbjct: 61  CSKFNVPYEIINFQKEYYNKVVSYTIKELKNGNTPSPDIFCNQRIKFGAFFEKI-NEQYD 120

Query: 202 YVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDD 261
            V +GHYA +        DK   L+ +KD +KDQ+YFLSHLSQ Q+ +L FPLG + K +
Sbjct: 121 LVVTGHYAKI-----QTKDKKFFLKQAKDKIKDQSYFLSHLSQKQMSKLYFPLGTLLKSE 180

Query: 262 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 321
           VR++A   NLPNK+RKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +G H G+W
Sbjct: 181 VRQIAKNINLPNKNRKDSQGICFLGKIKYNEFIKYHLGEKKGNIIEKETGKIIGTHNGYW 240

Query: 322 FYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGLPPDQ 381
           F+T+GQR+G++L  GPW+V+EKD++ N++++S N     + +R F V  + W++G P D 
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYSKQAKRKFLVHEIHWINGTPSD- 300

Query: 382 FTGLQCKVRHGPSFYSCNLTMEHNEDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGS 441
           F   + K+RHG   YSC L +       +++  + L++ D G++ GQFA FYK   C+G 
Sbjct: 301 FENFKIKIRHGEKKYSCKLKLI-----AKNLIEISLNKKDYGISPGQFAIFYKNTECLGG 352

Query: 442 GVILE 446
             I +
Sbjct: 361 AKIFK 352

[Close]

BLAST of Chy5G091750 vs. ExPASy Swiss-Prot
Match: O51625 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680) OX=224326 GN=mnmA PE=3 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 7.7e-100
Identity = 167/365 (45.75%), Postives = 250/365 (68.49%), Query Frame = 0


Query: 82  LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 141
           +K+AVLLSGGVDSSVAL R+++    +   +YLKIW +++  ++   CPW +DL Y +A+
Sbjct: 1   MKIAVLLSGGVDSSVALYRIINKGYSNIKCYYLKIWVEDEL-SYIGNCPWQEDLNYVEAI 60

Query: 142 CDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 201
           C++ +VP E+++  +EY+  VVSY IEE + G TP+PD+ CN RIKFGAF + I N ++D
Sbjct: 61  CNKFNVPYEIINFQKEYYNKVVSYTIEELKNGNTPSPDIFCNQRIKFGAFFEKI-NSQYD 120

Query: 202 YVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDD 261
            V +GHYA +        +   +L+ +KD +KDQ+YFLSHLSQ Q+ +L FPLG + K +
Sbjct: 121 LVVTGHYAKI-----QIKESKFLLKQAKDKIKDQSYFLSHLSQKQMSKLYFPLGTLLKSE 180

Query: 262 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 321
           VR++A   NLPNKDRKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +G H G+W
Sbjct: 181 VRQIAKNINLPNKDRKDSQGICFLGKIKYNEFIKYHLGEKKGNIIEKETGKIIGIHNGYW 240

Query: 322 FYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGLPPDQ 381
           F+T+GQR+G++L  GPW+V+EKD++ N++++S N     + +R F V  + W++  P + 
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYLKQAKRKFLVHEIHWINDTPTN- 300

Query: 382 FTGLQCKVRHGPSFYSCNLTMEHNEDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGS 441
           F   + K+RHG   YSC L +  N     ++  + L++ DQG++ GQFA FYK   C+G 
Sbjct: 301 FENFKIKIRHGEKKYSCKLKLITN-----NLMEISLNKKDQGISPGQFAIFYKNTECLGG 352

Query: 442 GVILE 446
             I +
Sbjct: 361 AKIFK 352

[Close]

BLAST of Chy5G091750 vs. ExPASy Swiss-Prot
Match: B7J2N7 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ZS7) OX=445985 GN=mnmA PE=3 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 7.7e-100
Identity = 167/365 (45.75%), Postives = 250/365 (68.49%), Query Frame = 0


Query: 82  LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 141
           +K+AVLLSGGVDSSVAL R+++    +   +YLKIW +++  ++   CPW +DL Y +A+
Sbjct: 1   MKIAVLLSGGVDSSVALYRIINKGYSNIKCYYLKIWVEDEL-SYIGNCPWQEDLNYVEAI 60

Query: 142 CDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 201
           C++ +VP E+++  +EY+  VVSY IEE + G TP+PD+ CN RIKFGAF + I N ++D
Sbjct: 61  CNKFNVPYEIINFQKEYYNKVVSYTIEELKNGNTPSPDIFCNQRIKFGAFFEKI-NSQYD 120

Query: 202 YVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDD 261
            V +GHYA +        +   +L+ +KD +KDQ+YFLSHLSQ Q+ +L FPLG + K +
Sbjct: 121 LVVTGHYAKI-----QIKESKFLLKQAKDKIKDQSYFLSHLSQKQISKLYFPLGTLLKSE 180

Query: 262 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 321
           VR++A   NLPNKDRKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +G H G+W
Sbjct: 181 VRQIAKNINLPNKDRKDSQGICFLGKIKYNEFIKYHLGEKKGNIIEKETGKIIGIHNGYW 240

Query: 322 FYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGLPPDQ 381
           F+T+GQR+G++L  GPW+V+EKD++ N++++S N     + +R F V  + W++  P + 
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYLKQAKRKFLVHEIHWINDTPTN- 300

Query: 382 FTGLQCKVRHGPSFYSCNLTMEHNEDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGS 441
           F   + K+RHG   YSC L +  N     ++  + L++ DQG++ GQFA FYK   C+G 
Sbjct: 301 FENFKIKIRHGEKKYSCKLKLITN-----NLMEISLNKKDQGISPGQFAIFYKNTECLGG 352

Query: 442 GVILE 446
             I +
Sbjct: 361 AKIFK 352

[Close]

BLAST of Chy5G091750 vs. ExPASy Swiss-Prot
Match: Q660I8 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia bavariensis (strain ATCC BAA-2496 / DSM 23469 / PBi) OX=290434 GN=mnmA PE=3 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 1.0e-99
Identity = 166/365 (45.48%), Postives = 250/365 (68.49%), Query Frame = 0


Query: 82  LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 141
           +K+AVLLSGGVDSS+AL ++++    +   +YLKIW +++  ++   CPW +DL Y +AV
Sbjct: 1   MKIAVLLSGGVDSSIALYKVINKGYTNIKCYYLKIWLEDEL-SYIGNCPWQEDLNYVEAV 60

Query: 142 CDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 201
           C++ ++P E+++  +EY+  VV Y IEE + G TP+PD+ CN RIKFGAF + I N ++D
Sbjct: 61  CNKFNIPYEIINFQKEYYNKVVIYTIEELKNGNTPSPDIFCNQRIKFGAFFEKI-NEQYD 120

Query: 202 YVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDD 261
            V +GHYA +        +K   L+ +KD +KDQ+YFLSHLSQ Q+ +L FPLG + K +
Sbjct: 121 LVVTGHYARI-----QIKEKKFFLKQAKDKIKDQSYFLSHLSQEQMSKLYFPLGTLFKSE 180

Query: 262 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 321
           VR++A   NLPNKDRKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +G H G+W
Sbjct: 181 VRQIAKSINLPNKDRKDSQGICFLGKIKYNEFIKYHLGEKKGNIVEKETGKIIGIHNGYW 240

Query: 322 FYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGLPPDQ 381
           F+T+GQR+G++L  GPW+V+EKD++ N++++S N     + +R F V  + W++G+P D 
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYLKQAKRKFLVHEIHWINGMPSD- 300

Query: 382 FTGLQCKVRHGPSFYSCNLTMEHNEDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGS 441
           F   + K+RHG   YSC L +  N     ++  + L++ D G++ GQFA FYK   C+G 
Sbjct: 301 FENFKIKIRHGEKKYSCKLRLITN-----NLIEIFLNKKDHGISPGQFAIFYKNTECLGG 352

Query: 442 GVILE 446
             I +
Sbjct: 361 AKIFK 352

[Close]

BLAST of Chy5G091750 vs. ExPASy Swiss-Prot
Match: B2S125 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia hermsii (strain HS1 / DAH) OX=314723 GN=mnmA PE=3 SV=2)

HSP 1 Score: 353.2 bits (905), Expect = 5.2e-96
Identity = 166/366 (45.36%), Postives = 244/366 (66.67%), Query Frame = 0


Query: 82  LKVAVLLSGGVDSSVALRLLHAAGH-SCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 141
           +K+AVLLSGGVDSSVAL  +   G+ +   +YLKIW +++  ++  ECPW++D+ Y +AV
Sbjct: 1   MKIAVLLSGGVDSSVALYTMIQKGYKNIKCYYLKIWLEDEL-SYIGECPWEEDINYVEAV 60

Query: 142 CDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 201
           C + +VP E++ L +EY++ VV+Y IEE + G TP+PD+ CN RIKFGAF D I N  +D
Sbjct: 61  CTKFNVPYEIISLQDEYYKRVVTYAIEELKIGNTPSPDIFCNQRIKFGAFFDKI-NEHYD 120

Query: 202 YVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDD 261
            + +GHYA +     +  +   +L+ +KD +KDQ+YFLSHLS+ Q+ +L FPLG + K +
Sbjct: 121 LIVTGHYAKI-----ENKNNHYILKQAKDKIKDQSYFLSHLSREQISKLHFPLGDLLKTE 180

Query: 262 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 321
           +R++A K +LPNK+RKDSQGICFLGKIK+ EF+  H+GE +G I+E E+G  LG H G+W
Sbjct: 181 IRQIAHKIDLPNKNRKDSQGICFLGKIKYDEFIKYHLGELKGNIIEQETGKILGTHNGYW 240

Query: 322 FYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGLPPDQ 381
           F+TIGQR+G++L  GPW+V EKDI+NN++++S +     + +  F V    W++   P  
Sbjct: 241 FFTIGQRKGIKLSHGPWFVTEKDIQNNIIYISNSVNYLAQGKHQFLVHKTNWIN--KPVN 300

Query: 382 FTGLQCKVRHGPSFYSCNLTMEHNEDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGS 441
              L  K+RHG     C + M  N     D+  V L E D G++ GQF  FY+   C+G 
Sbjct: 301 NIDLSAKIRHGEKKIKCKIEMLKN-----DIIKVDLEEKDYGISPGQFCIFYQEDECLGG 352

Query: 442 GVILES 447
             IL++
Sbjct: 361 AQILQT 352

[Close]

BLAST of Chy5G091750 vs. ExPASy TrEMBL
Match: A0A0A0KX16 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis sativus OX=3659 GN=Csa_4G291390 PE=3 SV=1)

HSP 1 Score: 1077.8 bits (2786), Expect = 0.0e+00
Identity = 526/538 (97.77%), Postives = 532/538 (98.88%), Query Frame = 0


Query: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRRHNPRLFLTRFSASSAVHNTSLSSS 60
           MMLRLRVNPAVWCSVPISHFTSLPL+PSILRPLLRRHNPRLFLTRFSASSAVHNTSLSSS
Sbjct: 1   MMLRLRVNPAVWCSVPISHFTSLPLTPSILRPLLRRHNPRLFLTRFSASSAVHNTSLSSS 60

Query: 61  PKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120
            KPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED
Sbjct: 61  SKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120

Query: 121 FENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 180
           FENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL
Sbjct: 121 FENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 180

Query: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 240
           CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH
Sbjct: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 240

Query: 241 LSQNQLKRLLFPLGCIPKDDVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 300
           LSQNQLKRLLFPLGCIPKD+VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED
Sbjct: 241 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 300

Query: 301 EGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 360
           EGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK
Sbjct: 301 EGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 360

Query: 361 RRRVFRVGSLKWLSGLPPDQFTGLQCKVRHGPSFYSCNLTMEHNEDGHEDVAVVHLSEDD 420
           RRRVFRVGSLKWLSG PPDQFT LQCKVRHGPSFYSCNLTMEH+EDGHEDVAVVHLSEDD
Sbjct: 361 RRRVFRVGSLKWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSEDD 420

Query: 421 QGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAKMEDKSKLGKPIKIKVKP 480
           QGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENA+MEDKSKLGKPIKIKVKP
Sbjct: 421 QGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKP 480

Query: 481 ESGTKESKAKDDTKIQDELRNEEATVVEQIREVSPDEAMATSSMKWLGSLKKKLLQIF 539
           ESG KESKAKDDT+I D+LRNEEATVVEQIREVSPDEAMAT SMKWLGSLKKKLLQIF
Sbjct: 481 ESGMKESKAKDDTEIHDKLRNEEATVVEQIREVSPDEAMATPSMKWLGSLKKKLLQIF 538

[Close]

BLAST of Chy5G091750 vs. ExPASy TrEMBL
Match: A0A5A7TDN6 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00570 PE=3 SV=1)

HSP 1 Score: 1065.8 bits (2755), Expect = 5.8e-308
Identity = 519/539 (96.29%), Postives = 529/539 (98.14%), Query Frame = 0


Query: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRRHNPRLFLTRFSASSAV-HNTSLSS 60
           MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLR HNPRLF TRFSASSAV HNTS SS
Sbjct: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60

Query: 61  SPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120
            PKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE
Sbjct: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120

Query: 121 DFENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180
           DFENFWSECPWDDDLKYAKAVCDQ+DVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV
Sbjct: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180

Query: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240
           LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS
Sbjct: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240

Query: 241 HLSQNQLKRLLFPLGCIPKDDVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300
           HLSQNQLKRLLFPLGCIPKD+VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE
Sbjct: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300

Query: 301 DEGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360
           DEGVILEAESGDFLGKHRGFWFYT+GQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD
Sbjct: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360

Query: 361 KRRRVFRVGSLKWLSGLPPDQFTGLQCKVRHGPSFYSCNLTMEHNEDGHEDVAVVHLSED 420
           KRRRVFRVGSLKWLSG+PPDQFTGLQCKVRHGP FYSCNLTMEHNEDGHEDVAVVHLSED
Sbjct: 361 KRRRVFRVGSLKWLSGMPPDQFTGLQCKVRHGPGFYSCNLTMEHNEDGHEDVAVVHLSED 420

Query: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAKMEDKSKLGKPIKIKVK 480
           DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENA+MEDKSKLGKPIKIKVK
Sbjct: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480

Query: 481 PESGTKESKAKDDTKIQDELRNEEATVVEQIREVSPDEAMATSSMKWLGSLKKKLLQIF 539
           P+SG  ESKAKDDTKIQDE RNEEATVVEQ+R+VSPDEAMAT+ MKWLGSL+KKLLQIF
Sbjct: 481 PDSGMNESKAKDDTKIQDESRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 539

[Close]

BLAST of Chy5G091750 vs. ExPASy TrEMBL
Match: A0A5D3B8G8 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G001100 PE=3 SV=1)

HSP 1 Score: 1061.2 bits (2743), Expect = 1.4e-306
Identity = 516/539 (95.73%), Postives = 529/539 (98.14%), Query Frame = 0


Query: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRRHNPRLFLTRFSASSAV-HNTSLSS 60
           MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLR HNPRLF TRFSASSAV HNTS SS
Sbjct: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60

Query: 61  SPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120
            PKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE
Sbjct: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120

Query: 121 DFENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180
           DFENFWSECPWDDDLKYAKAVCDQ+DVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV
Sbjct: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180

Query: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240
           LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS
Sbjct: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240

Query: 241 HLSQNQLKRLLFPLGCIPKDDVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300
           HLSQNQLKRLLFPLGCIPKD+VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE
Sbjct: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300

Query: 301 DEGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360
           DEGVILEAESGDFLGKHRGFWFYT+GQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD
Sbjct: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360

Query: 361 KRRRVFRVGSLKWLSGLPPDQFTGLQCKVRHGPSFYSCNLTMEHNEDGHEDVAVVHLSED 420
           KRRRVFRVGSLKWLSG+PPD+FTGLQCKVRHGP FYSCNLTMEHN+DGHEDVAVVHLSED
Sbjct: 361 KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED 420

Query: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAKMEDKSKLGKPIKIKVK 480
           DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENA+MEDKSKLGKPIKIKVK
Sbjct: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480

Query: 481 PESGTKESKAKDDTKIQDELRNEEATVVEQIREVSPDEAMATSSMKWLGSLKKKLLQIF 539
           P+SG  ESKAKDDTKIQD+ RNEEATVVEQ+R+VSPDEAMAT+ MKWLGSL+KKLLQIF
Sbjct: 481 PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 539

[Close]

BLAST of Chy5G091750 vs. ExPASy TrEMBL
Match: A0A1S3BNL8 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo OX=3656 GN=LOC103491516 PE=3 SV=1)

HSP 1 Score: 1061.2 bits (2743), Expect = 1.4e-306
Identity = 516/539 (95.73%), Postives = 529/539 (98.14%), Query Frame = 0


Query: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRRHNPRLFLTRFSASSAV-HNTSLSS 60
           MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLR HNPRLF TRFSASSAV HNTS SS
Sbjct: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60

Query: 61  SPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120
            PKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE
Sbjct: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120

Query: 121 DFENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180
           DFENFWSECPWDDDLKYAKAVCDQ+DVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV
Sbjct: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180

Query: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240
           LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS
Sbjct: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240

Query: 241 HLSQNQLKRLLFPLGCIPKDDVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300
           HLSQNQLKRLLFPLGCIPKD+VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE
Sbjct: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300

Query: 301 DEGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360
           DEGVILEAESGDFLGKHRGFWFYT+GQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD
Sbjct: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360

Query: 361 KRRRVFRVGSLKWLSGLPPDQFTGLQCKVRHGPSFYSCNLTMEHNEDGHEDVAVVHLSED 420
           KRRRVFRVGSLKWLSG+PPD+FTGLQCKVRHGP FYSCNLTMEHN+DGHEDVAVVHLSED
Sbjct: 361 KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED 420

Query: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAKMEDKSKLGKPIKIKVK 480
           DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENA+MEDKSKLGKPIKIKVK
Sbjct: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480

Query: 481 PESGTKESKAKDDTKIQDELRNEEATVVEQIREVSPDEAMATSSMKWLGSLKKKLLQIF 539
           P+SG  ESKAKDDTKIQD+ RNEEATVVEQ+R+VSPDEAMAT+ MKWLGSL+KKLLQIF
Sbjct: 481 PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 539

[Close]

BLAST of Chy5G091750 vs. ExPASy TrEMBL
Match: A0A6J1FY63 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucurbita moschata OX=3662 GN=LOC111448983 PE=3 SV=1)

HSP 1 Score: 988.0 bits (2553), Expect = 1.5e-284
Identity = 482/537 (89.76%), Postives = 504/537 (93.85%), Query Frame = 0


Query: 2   MLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRRHNPRLFLTRFSASSAVHNTSLSSSP 61
           MLRLRVNP VWC++PISHFTSLPL PSILRP+LRRH PRLFLTRFSASSAVH  S SSS 
Sbjct: 1   MLRLRVNP-VWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESSSA 60

Query: 62  KPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 121
           K LSD D  PFLSCSMP +PLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF
Sbjct: 61  KGLSDGDLEPFLSCSMPRHPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 120

Query: 122 ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 181
           ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC
Sbjct: 121 ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 180

Query: 182 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHL 241
           NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQM+KPSVLELSKDMVKDQTYFLSHL
Sbjct: 181 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSHL 240

Query: 242 SQNQLKRLLFPLGCIPKDDVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 301
           SQNQLKRLLFPLGCIPKD+VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE
Sbjct: 241 SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 300

Query: 302 GVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKR 361
           GVILEAESGDFLGKHRGFWFYTIGQRQGL+LPGGPWYVVEKDI+NNVVFVSRNYFS DKR
Sbjct: 301 GVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKR 360

Query: 362 RRVFRVGSLKWLSGLPPDQFTGLQCKVRHGPSFYSCNLTMEHNEDGHEDVAVVHLSEDDQ 421
           RR+FRVGSLKWLSGL PDQFT LQCKVRHGPSFYSCNLTME NED  EDVAVVHLSEDDQ
Sbjct: 361 RRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVHLSEDDQ 420

Query: 422 GLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAKMEDKSKLGKPIKIKVKPE 481
           GLAAGQFAAFY+GR CIGSGVILESWDDQGFPVC KA+E A+MEDKSKLGKPIKIKVKPE
Sbjct: 421 GLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPE 480

Query: 482 SGTKESKAKDDTKIQDELRNEEATVVEQIREVSPDEAMATSSMKWLGSLKKKLLQIF 539
           + TKE + KDDTKI   L+N++ TVVEQ  EV+ DEA+ T+ MKWLGSL+KKLLQ+F
Sbjct: 481 NATKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 536

[Close]

BLAST of Chy5G091750 vs. NCBI nr
Match: XP_004142116.1 (uncharacterized protein LOC101204779 isoform X1 [Cucumis sativus] >KGN54175.1 hypothetical protein Csa_017959 [Cucumis sativus])

HSP 1 Score: 1073 bits (2774), Expect = 0.0
Identity = 526/538 (97.77%), Postives = 532/538 (98.88%), Query Frame = 0


Query: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRRHNPRLFLTRFSASSAVHNTSLSSS 60
           MMLRLRVNPAVWCSVPISHFTSLPL+PSILRPLLRRHNPRLFLTRFSASSAVHNTSLSSS
Sbjct: 1   MMLRLRVNPAVWCSVPISHFTSLPLTPSILRPLLRRHNPRLFLTRFSASSAVHNTSLSSS 60

Query: 61  PKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120
            KPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED
Sbjct: 61  SKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120

Query: 121 FENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 180
           FENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL
Sbjct: 121 FENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 180

Query: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 240
           CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH
Sbjct: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 240

Query: 241 LSQNQLKRLLFPLGCIPKDDVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 300
           LSQNQLKRLLFPLGCIPKD+VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED
Sbjct: 241 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 300

Query: 301 EGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 360
           EGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK
Sbjct: 301 EGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 360

Query: 361 RRRVFRVGSLKWLSGLPPDQFTGLQCKVRHGPSFYSCNLTMEHNEDGHEDVAVVHLSEDD 420
           RRRVFRVGSLKWLSG PPDQFT LQCKVRHGPSFYSCNLTMEH+EDGHEDVAVVHLSEDD
Sbjct: 361 RRRVFRVGSLKWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSEDD 420

Query: 421 QGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAKMEDKSKLGKPIKIKVKP 480
           QGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENA+MEDKSKLGKPIKIKVKP
Sbjct: 421 QGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKP 480

Query: 481 ESGTKESKAKDDTKIQDELRNEEATVVEQIREVSPDEAMATSSMKWLGSLKKKLLQIF 538
           ESG KESKAKDDT+I D+LRNEEATVVEQIREVSPDEAMAT SMKWLGSLKKKLLQIF
Sbjct: 481 ESGMKESKAKDDTEIHDKLRNEEATVVEQIREVSPDEAMATPSMKWLGSLKKKLLQIF 538

[Close]

BLAST of Chy5G091750 vs. NCBI nr
Match: KAA0041410.1 (tRNA-specific 2-thiouridylase MnmA [Cucumis melo var. makuwa])

HSP 1 Score: 1061 bits (2743), Expect = 0.0
Identity = 519/539 (96.29%), Postives = 529/539 (98.14%), Query Frame = 0


Query: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRRHNPRLFLTRFSASSAV-HNTSLSS 60
           MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLR HNPRLF TRFSASSAV HNTS SS
Sbjct: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60

Query: 61  SPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120
            PKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE
Sbjct: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120

Query: 121 DFENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180
           DFENFWSECPWDDDLKYAKAVCDQ+DVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV
Sbjct: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180

Query: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240
           LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS
Sbjct: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240

Query: 241 HLSQNQLKRLLFPLGCIPKDDVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300
           HLSQNQLKRLLFPLGCIPKD+VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE
Sbjct: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300

Query: 301 DEGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360
           DEGVILEAESGDFLGKHRGFWFYT+GQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD
Sbjct: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360

Query: 361 KRRRVFRVGSLKWLSGLPPDQFTGLQCKVRHGPSFYSCNLTMEHNEDGHEDVAVVHLSED 420
           KRRRVFRVGSLKWLSG+PPDQFTGLQCKVRHGP FYSCNLTMEHNEDGHEDVAVVHLSED
Sbjct: 361 KRRRVFRVGSLKWLSGMPPDQFTGLQCKVRHGPGFYSCNLTMEHNEDGHEDVAVVHLSED 420

Query: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAKMEDKSKLGKPIKIKVK 480
           DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENA+MEDKSKLGKPIKIKVK
Sbjct: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480

Query: 481 PESGTKESKAKDDTKIQDELRNEEATVVEQIREVSPDEAMATSSMKWLGSLKKKLLQIF 538
           P+SG  ESKAKDDTKIQDE RNEEATVVEQ+R+VSPDEAMAT+ MKWLGSL+KKLLQIF
Sbjct: 481 PDSGMNESKAKDDTKIQDESRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 539

[Close]

BLAST of Chy5G091750 vs. NCBI nr
Match: XP_008449720.1 (PREDICTED: tRNA-specific 2-thiouridylase MnmA [Cucumis melo] >TYJ96142.1 tRNA-specific 2-thiouridylase MnmA [Cucumis melo var. makuwa])

HSP 1 Score: 1056 bits (2731), Expect = 0.0
Identity = 516/539 (95.73%), Postives = 529/539 (98.14%), Query Frame = 0


Query: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRRHNPRLFLTRFSASSAV-HNTSLSS 60
           MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLR HNPRLF TRFSASSAV HNTS SS
Sbjct: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60

Query: 61  SPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120
            PKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE
Sbjct: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120

Query: 121 DFENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180
           DFENFWSECPWDDDLKYAKAVCDQ+DVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV
Sbjct: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180

Query: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240
           LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS
Sbjct: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240

Query: 241 HLSQNQLKRLLFPLGCIPKDDVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300
           HLSQNQLKRLLFPLGCIPKD+VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE
Sbjct: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300

Query: 301 DEGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360
           DEGVILEAESGDFLGKHRGFWFYT+GQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD
Sbjct: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360

Query: 361 KRRRVFRVGSLKWLSGLPPDQFTGLQCKVRHGPSFYSCNLTMEHNEDGHEDVAVVHLSED 420
           KRRRVFRVGSLKWLSG+PPD+FTGLQCKVRHGP FYSCNLTMEHN+DGHEDVAVVHLSED
Sbjct: 361 KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED 420

Query: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAKMEDKSKLGKPIKIKVK 480
           DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENA+MEDKSKLGKPIKIKVK
Sbjct: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480

Query: 481 PESGTKESKAKDDTKIQDELRNEEATVVEQIREVSPDEAMATSSMKWLGSLKKKLLQIF 538
           P+SG  ESKAKDDTKIQD+ RNEEATVVEQ+R+VSPDEAMAT+ MKWLGSL+KKLLQIF
Sbjct: 481 PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 539

[Close]

BLAST of Chy5G091750 vs. NCBI nr
Match: XP_038901654.1 (tRNA-specific 2-thiouridylase MnmA [Benincasa hispida])

HSP 1 Score: 1015 bits (2624), Expect = 0.0
Identity = 494/537 (91.99%), Postives = 514/537 (95.72%), Query Frame = 0


Query: 2   MLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRRHNPRLFLTRFSASSAVHNTSLSSSP 61
           MLRLRV P VWCS+PISHFTSLPLSPSILRP+LRR NPRLFLTRFSASSA+  T  SSSP
Sbjct: 1   MLRLRVKPVVWCSLPISHFTSLPLSPSILRPVLRRRNPRLFLTRFSASSALCKTLESSSP 60

Query: 62  KPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 121
           K LSDAD +PFLSCSMP+YPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF
Sbjct: 61  KGLSDADLVPFLSCSMPSYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 120

Query: 122 ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 181
           ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC
Sbjct: 121 ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 180

Query: 182 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHL 241
           NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHL
Sbjct: 181 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHL 240

Query: 242 SQNQLKRLLFPLGCIPKDDVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 301
           SQNQLKRLLFPLGCIPKD+VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE
Sbjct: 241 SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 300

Query: 302 GVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKR 361
           GVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKR
Sbjct: 301 GVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKR 360

Query: 362 RRVFRVGSLKWLSGLPPDQFTGLQCKVRHGPSFYSCNLTMEHNEDGHEDVAVVHLSEDDQ 421
           RRVFRVGSLKWL+G+PPDQF+GLQCKVRHGPSFYSCNLTMEHNEDG ED+AVVHLSEDDQ
Sbjct: 361 RRVFRVGSLKWLTGMPPDQFSGLQCKVRHGPSFYSCNLTMEHNEDGREDIAVVHLSEDDQ 420

Query: 422 GLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAKMEDKSKLGKPIKIKVKPE 481
           GLAAGQFAAFYKGR CIGSGVILESWDDQGFPVC KA+ENA+MEDKSKLGKPIKIKVKPE
Sbjct: 421 GLAAGQFAAFYKGRRCIGSGVILESWDDQGFPVCPKAVENAQMEDKSKLGKPIKIKVKPE 480

Query: 482 SGTKESKAKDDTKIQDELRNEEATVVEQIREVSPDEAMATSSMKWLGSLKKKLLQIF 538
           S  KES+AKDDTKI  E  N+E TV++QI EVS DEA+ T+ MKWLGSL+KKLLQ+F
Sbjct: 481 SVLKESEAKDDTKIHGESNNQETTVIKQIGEVSSDEAITTAPMKWLGSLRKKLLQVF 537

[Close]

BLAST of Chy5G091750 vs. NCBI nr
Match: XP_022944568.1 (uncharacterized protein LOC111448983 [Cucurbita moschata])

HSP 1 Score: 983 bits (2541), Expect = 0.0
Identity = 482/537 (89.76%), Postives = 504/537 (93.85%), Query Frame = 0


Query: 2   MLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRRHNPRLFLTRFSASSAVHNTSLSSSP 61
           MLRLRVNP VWC++PISHFTSLPL PSILRP+LRRH PRLFLTRFSASSAVH  S SSS 
Sbjct: 1   MLRLRVNP-VWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESSSA 60

Query: 62  KPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 121
           K LSD D  PFLSCSMP +PLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF
Sbjct: 61  KGLSDGDLEPFLSCSMPRHPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 120

Query: 122 ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 181
           ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC
Sbjct: 121 ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 180

Query: 182 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHL 241
           NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQM+KPSVLELSKDMVKDQTYFLSHL
Sbjct: 181 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSHL 240

Query: 242 SQNQLKRLLFPLGCIPKDDVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 301
           SQNQLKRLLFPLGCIPKD+VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE
Sbjct: 241 SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 300

Query: 302 GVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKR 361
           GVILEAESGDFLGKHRGFWFYTIGQRQGL+LPGGPWYVVEKDI+NNVVFVSRNYFS DKR
Sbjct: 301 GVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKR 360

Query: 362 RRVFRVGSLKWLSGLPPDQFTGLQCKVRHGPSFYSCNLTMEHNEDGHEDVAVVHLSEDDQ 421
           RR+FRVGSLKWLSGL PDQFT LQCKVRHGPSFYSCNLTME NED  EDVAVVHLSEDDQ
Sbjct: 361 RRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVHLSEDDQ 420

Query: 422 GLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAKMEDKSKLGKPIKIKVKPE 481
           GLAAGQFAAFY+GR CIGSGVILESWDDQGFPVC KA+E A+MEDKSKLGKPIKIKVKPE
Sbjct: 421 GLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPE 480

Query: 482 SGTKESKAKDDTKIQDELRNEEATVVEQIREVSPDEAMATSSMKWLGSLKKKLLQIF 538
           + TKE + KDDTKI   L+N++ TVVEQ  EV+ DEA+ T+ MKWLGSL+KKLLQ+F
Sbjct: 481 NATKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 536

[Close]

BLAST of Chy5G091750 vs. TAIR 10
Match: AT1G51310.1 (transferases;tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferases )

HSP 1 Score: 708.4 bits (1827), Expect = 4.5e-204
Identity = 347/459 (75.60%), Postives = 385/459 (83.88%), Query Frame = 0


Query: 40  RLFLTRFSASSAVH---------NTSLSSSPKPLSDADFL--PFLSCSMPTYPLKVAVLL 99
           R+F + FS+SS+            T L SS +  S   F+   +LSCSMP  PL+VAVLL
Sbjct: 29  RVFPSFFSSSSSSQLPSPQSSNPITPLISSNRNYSRHAFIVDQYLSCSMPEKPLRVAVLL 88

Query: 100 SGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAVCDQVDVPL 159
           SGGVDSSVALRLLHAAGHSCTAFYLKIWFQE FENFW++CPW+DDLKYAK VC+QVDVPL
Sbjct: 89  SGGVDSSVALRLLHAAGHSCTAFYLKIWFQEGFENFWNQCPWEDDLKYAKHVCEQVDVPL 148

Query: 160 EVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYVASGHYA 219
           EVVHLT+EYW  VVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAIS+ME+DYV SGHYA
Sbjct: 149 EVVHLTDEYWERVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISDMEYDYVGSGHYA 208

Query: 220 NVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDDVRKLAAKF 279
            V+HP ADQ D  SVLELS+DMVKDQTYFLSHLSQ QLKRLLFPLGC+ KD+VRKLA +F
Sbjct: 209 KVVHPPADQNDASSVLELSQDMVKDQTYFLSHLSQTQLKRLLFPLGCVKKDEVRKLATQF 268

Query: 280 NLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFWFYTIGQRQ 339
           +LPNKDRKDSQGICFLGKIKFS+FV RHIGE EG+ILEAESGDFLG HRGFWFYTIGQRQ
Sbjct: 269 DLPNKDRKDSQGICFLGKIKFSDFVCRHIGEMEGIILEAESGDFLGNHRGFWFYTIGQRQ 328

Query: 340 GLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGLPPDQFTGLQCKV 399
           GLRLPGGPWYVVEKD KNNVVFVSRNY+S DKRRR+FRVGSL+W SG P  +   L+CKV
Sbjct: 329 GLRLPGGPWYVVEKDTKNNVVFVSRNYYSIDKRRRIFRVGSLRWNSGKPSGKVRELRCKV 388

Query: 400 RHGPSFYSCNLTMEHNEDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGSGVILESWD 459
           RHGP FYSC+  ME    G  DVAVVHL EDDQGLAAGQFAAFY+G +CIGSGVILESWD
Sbjct: 389 RHGPGFYSCSFEME----GDGDVAVVHLDEDDQGLAAGQFAAFYEGTTCIGSGVILESWD 448

Query: 460 DQGFPVCQKAIENAKMEDKSKLGKPIKIKVKPESGTKES 488
           DQ FPVC KA++ A +EDK+KLGKP+KI   P   + E+
Sbjct: 449 DQCFPVCAKALQLAALEDKTKLGKPVKIMTMPTMSSVEA 483

The following BLAST results are available for this feature:

BLAST of Chy5G091750 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0SMH1	3.5e-100	46.30	tRNA-specific 2-thiouridylase MnmA OS=Borrelia afzelii (strain PKo) OX=390236 GN...	[more]
O51625	7.7e-100	45.75	tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ATCC 35210 / ...	[more]
B7J2N7	7.7e-100	45.75	tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ZS7) OX=44598...	[more]
Q660I8	1.0e-99	45.48	tRNA-specific 2-thiouridylase MnmA OS=Borrelia bavariensis (strain ATCC BAA-2496...	[more]
B2S125	5.2e-96	45.36	tRNA-specific 2-thiouridylase MnmA OS=Borrelia hermsii (strain HS1 / DAH) OX=314...	[more]

BLAST of Chy5G091750 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KX16	0.0e+00	97.77	tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis sativus OX=3659 GN=Cs...	[more]
A0A5A7TDN6	5.8e-308	96.29	tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo var. makuwa OX=1...	[more]
A0A5D3B8G8	1.4e-306	95.73	tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo var. makuwa OX=1...	[more]
A0A1S3BNL8	1.4e-306	95.73	tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A6J1FY63	1.5e-284	89.76	tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucurbita moschata OX=3662 GN...	[more]

BLAST of Chy5G091750 vs. NCBI nr


Match Name	Identity	Description
XP_004142116.1	97.77	uncharacterized protein LOC101204779 isoform X1 [Cucumis sativus] >KGN54175.1 hy...	[more]
KAA0041410.1	96.29	tRNA-specific 2-thiouridylase MnmA [Cucumis melo var. makuwa]	[more]
XP_008449720.1	95.73	PREDICTED: tRNA-specific 2-thiouridylase MnmA [Cucumis melo] >TYJ96142.1 tRNA-sp...	[more]
XP_038901654.1	91.99	tRNA-specific 2-thiouridylase MnmA [Benincasa hispida]	[more]
XP_022944568.1	89.76	uncharacterized protein LOC111448983 [Cucurbita moschata]	[more]

BLAST of Chy5G091750 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G51310.1	4.5e-204	75.60	transferases;tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferases	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	2.40.30.10	Translation factors	coord: 362..451 e-value: 1.6E-13 score: 52.0
None	No IPR available	PFAM	PF03054	tRNA_Me_trans	coord: 83..443 e-value: 5.1E-126 score: 420.2
None	No IPR available	PANTHER	PTHR43052	FAMILY NOT NAMED	coord: 72..476
None	No IPR available	SUPERFAMILY	52402	Adenine nucleotide alpha hydrolases-like	coord: 83..328
IPR004506	tRNA-specific 2-thiouridylase	TIGRFAM	TIGR00420	TIGR00420	coord: 83..443 e-value: 2.8E-109 score: 363.1
IPR004506	tRNA-specific 2-thiouridylase	HAMAP	MF_00144	tRNA_thiouridyl_MnmA	coord: 82..444 score: 33.204365
IPR004506	tRNA-specific 2-thiouridylase	CDD	cd01998	tRNA_Me_trans	coord: 83..443 e-value: 1.4476E-159 score: 456.213
IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	GENE3D	3.40.50.620	HUPs	coord: 74..295 e-value: 4.7E-85 score: 286.5
IPR023382	Adenine nucleotide alpha hydrolase-like domains	GENE3D	2.30.30.280		coord: 296..355 e-value: 7.0E-19 score: 69.0

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy5G091750.1	Chy5G091750.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008033	tRNA processing
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0061708	tRNA-5-taurinomethyluridine 2-sulfurtransferase
molecular_function	GO:0000049	tRNA binding
molecular_function	GO:0016783	sulfurtransferase activity
molecular_function	GO:0016740	transferase activity