Chy4G087460 (gene) Cucumber (hystrix) v1

Overview
NameChy4G087460
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionCarbonic anhydrase
LocationchrH04: 25930742 .. 25932599 (-)
RNA-Seq ExpressionChy4G087460
SyntenyChy4G087460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGGCAGTAAGCCAAAGAACTTTTTCTTTGGCTTACCTTACCATCCTAACCTCTACCCTGCTCTGGGCATCTGTCTTATCACACCAACCTGGTAAGTACTTCTTTGAAGACTTAATAAAGCCAATATTACATGAAAATAGTTCAATTCCAAATTTCATTTCTAAGGTTATTTGGAAAAGGTTAATATTTAATTTTTATAGTTTATAATTACAATTTAGCTTTCTAAGGTTTGATAAAACTGAATATTTTGTTTTTCAAATTATAAGAACAGTGTGTGTTTGGAAGAAATTTGAGTAATGAAAATTTCAATTTAATCTTTGCTTCTTTTGATCCTAATGTCAAATTTGTAGGGGAGGTATGTGAGTTTGATTACATAGGAGGGAGCAATAAAGGGCCAACGAAATGGGGTGCAATCAAGGGAGAATGGTCCAAGTGTAACCTTGGCAAAATGCAATCTCCCATTGATCTATCAACCAATACAATCAATGTTGTTAATAGTTTGGGTACACTTCAGAGAAAATACAATCCCTTTTCTGCCATACTTAAGAATCGCGGCCACGATATTTCGGTATAAAAGAAAGAAACTAATAGATGTTAATTACCACTCAAGTTATACTCACAATTTAACACGTATACATTTAACACATATACAAGTTAAATACTTTTACCAATGAATTGATAATGATTGGATTCAAACTATACGCATCTAAATCATTACTTAAACTAAAATTTCAACTTCTGTCTAAGGTAATGAATCAATGTAACCTTTTGTTGTATGATTTTATGTGGTCGATAATTTTTTTTTTTTAATTATTAGAATGAGGATACTTAAAGTTAGTATTTTTCCTCTTGAAATAATACGATTGAATAGGTTGCATGGGAAGGAAATGTGGGATCAATTGAGATCAATGGCACAAAATATCTTCTTAAACAAATTCATTGGCACTCTCCTTCCGAACATACTCTTAATGGAAGAAGGTGATTATCAATATTTTTTCTCTTTCAACTTTTTAAAGTGTTGTTATGCATTTAATAATTTTTTTTTTTATTGAATGAATTTAGGTACGATTTAGAACTTCATGTGGTGCATGAAAGTTTAAATCCAAATGCCAAGTACGCTGTTGTTTCCTACTTGTACGAGATTGGTCTTCCTGATTTTTTCCTTTCAAAGGTAACAAATAATTGTAAATTTCAAGAACTTACTATATATATTGTGGCTAACACAATAGTTTTACTATGAGAAAAAGGTGAAATAAATAGAATATTAAATTAATGAAGACTAATTAACAAATAAATGAAATTGCAGGTGAGTAGAGGAATAAAGGAATTGAGTACTGGAAAGAAAGAGATAAAATTAGGAAGCATAAATCCTAATGAGATAAGAAACGCGGGTGGGATTAATTATTATCGATACATTGGCTCCCTCACTGTCCCTCCTTGTACTGAAGGTGTCGTTTGGAGCATTAACAAACAGGTGCTCTCTTTCTTCTTTCATTTCTTTCTTCTTCCCCATATATTTTTCATTATAAGTAATTGTTTTTTTTTTCTTAAATGGATATATATCAGATAGGAACTGTCTCACAAGAACAAGTGATGCTTCTGAAATCGGCTGTTGAACATGTAAGTTCTATAAGACTTCAAACTTTTCATCTTTTCTATTATAATTAAGTTGACATATATATATATATATTGGTTCATGATCTTATTATACTCAAATTATTTTTATAATTATTATAACTTTTAATATGTGAAACTGCAGTGTGCAGAGACAAATGCAAGACCAGTCCAACCACTTAATGGACGACAAGTTCAACTCTATTGTCCAAACCCATCATCCATTCCCATGCCCAATTGA

mRNA sequence

ATGTTGGCAGTAAGCCAAAGAACTTTTTCTTTGGCTTACCTTACCATCCTAACCTCTACCCTGCTCTGGGCATCTGTCTTATCACACCAACCTGGGGAGGTATGTGAGTTTGATTACATAGGAGGGAGCAATAAAGGGCCAACGAAATGGGGTGCAATCAAGGGAGAATGGTCCAAGTGTAACCTTGGCAAAATGCAATCTCCCATTGATCTATCAACCAATACAATCAATGTTGTTAATAGTTTGGGTACACTTCAGAGAAAATACAATCCCTTTTCTGCCATACTTAAGAATCGCGGCCACGATATTTCGGTTGCATGGGAAGGAAATGTGGGATCAATTGAGATCAATGGCACAAAATATCTTCTTAAACAAATTCATTGGCACTCTCCTTCCGAACATACTCTTAATGGAAGAAGGTACGATTTAGAACTTCATGTGGTGCATGAAAGTTTAAATCCAAATGCCAAGTACGCTGTTGTTTCCTACTTGTACGAGATTGGTCTTCCTGATTTTTTCCTTTCAAAGGTGAGTAGAGGAATAAAGGAATTGAGTACTGGAAAGAAAGAGATAAAATTAGGAAGCATAAATCCTAATGAGATAAGAAACGCGGGTGGGATTAATTATTATCGATACATTGGCTCCCTCACTGTCCCTCCTTGTACTGAAGGTGTCGTTTGGAGCATTAACAAACAGATAGGAACTGTCTCACAAGAACAAGTGATGCTTCTGAAATCGGCTGTTGAACATTGTGCAGAGACAAATGCAAGACCAGTCCAACCACTTAATGGACGACAAGTTCAACTCTATTGTCCAAACCCATCATCCATTCCCATGCCCAATTGA

Coding sequence (CDS)

ATGTTGGCAGTAAGCCAAAGAACTTTTTCTTTGGCTTACCTTACCATCCTAACCTCTACCCTGCTCTGGGCATCTGTCTTATCACACCAACCTGGGGAGGTATGTGAGTTTGATTACATAGGAGGGAGCAATAAAGGGCCAACGAAATGGGGTGCAATCAAGGGAGAATGGTCCAAGTGTAACCTTGGCAAAATGCAATCTCCCATTGATCTATCAACCAATACAATCAATGTTGTTAATAGTTTGGGTACACTTCAGAGAAAATACAATCCCTTTTCTGCCATACTTAAGAATCGCGGCCACGATATTTCGGTTGCATGGGAAGGAAATGTGGGATCAATTGAGATCAATGGCACAAAATATCTTCTTAAACAAATTCATTGGCACTCTCCTTCCGAACATACTCTTAATGGAAGAAGGTACGATTTAGAACTTCATGTGGTGCATGAAAGTTTAAATCCAAATGCCAAGTACGCTGTTGTTTCCTACTTGTACGAGATTGGTCTTCCTGATTTTTTCCTTTCAAAGGTGAGTAGAGGAATAAAGGAATTGAGTACTGGAAAGAAAGAGATAAAATTAGGAAGCATAAATCCTAATGAGATAAGAAACGCGGGTGGGATTAATTATTATCGATACATTGGCTCCCTCACTGTCCCTCCTTGTACTGAAGGTGTCGTTTGGAGCATTAACAAACAGATAGGAACTGTCTCACAAGAACAAGTGATGCTTCTGAAATCGGCTGTTGAACATTGTGCAGAGACAAATGCAAGACCAGTCCAACCACTTAATGGACGACAAGTTCAACTCTATTGTCCAAACCCATCATCCATTCCCATGCCCAATTGA

Protein sequence

MLAVSQRTFSLAYLTILTSTLLWASVLSHQPGEVCEFDYIGGSNKGPTKWGAIKGEWSKCNLGKMQSPIDLSTNTINVVNSLGTLQRKYNPFSAILKNRGHDISVAWEGNVGSIEINGTKYLLKQIHWHSPSEHTLNGRRYDLELHVVHESLNPNAKYAVVSYLYEIGLPDFFLSKVSRGIKELSTGKKEIKLGSINPNEIRNAGGINYYRYIGSLTVPPCTEGVVWSINKQIGTVSQEQVMLLKSAVEHCAETNARPVQPLNGRQVQLYCPNPSSIPMPN*
Homology
BLAST of Chy4G087460 vs. ExPASy Swiss-Prot
Match: Q8L817 (Alpha carbonic anhydrase 7 OS=Arabidopsis thaliana OX=3702 GN=ACA7 PE=2 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 1.1e-65
Identity = 134/270 (49.63%), Postives = 176/270 (65.19%), Query Frame = 0

Query: 9   FSLAYLTILTSTLLWASVLSHQPGEV---CEFDYIGGSNKGPTKWGAIKGEWSKCNLGKM 68
           F +A  +I T   + ++  SH  GEV    EF+Y     KGP +WG +K EW  C  G+M
Sbjct: 11  FFVALFSIFTIVSISSAASSH--GEVEDEREFNYKKNDEKGPERWGELKPEWEMCGKGEM 70

Query: 69  QSPIDLSTNTINVVNSLGTLQRKYNPFSAILKNRGHDISVAWEGNVGSIEINGTKYLLKQ 128
           QSPIDL    +N+V+ LG L R YNP +A LKNRGHDI + +E   G+I+ING +Y L+Q
Sbjct: 71  QSPIDLMNERVNIVSHLGRLNRDYNPSNATLKNRGHDIMLKFEDGAGTIKINGFEYELQQ 130

Query: 129 IHWHSPSEHTLNGRRYDLELHVVHESLNPNAKYAVVSYLYEIGLPDFF---LSKVSRGIK 188
           +HWHSPSEHT+NGRR+ LELH+VHE    N + AVV+ LY+IG  D F   L K   GI 
Sbjct: 131 LHWHSPSEHTINGRRFALELHMVHE--GRNRRMAVVTVLYKIGRADTFIRSLEKELEGIA 190

Query: 189 ELSTGKKEIKLGSINPNEIRNAGGINYYRYIGSLTVPPCTEGVVWSINKQIGTVSQEQVM 248
           E+   +K +  G I+P +I+  G   YYRY GSLT PPCT+ V WS+ +++ TV+++QV 
Sbjct: 191 EMEEAEKNV--GMIDPTKIK-IGSRKYYRYTGSLTTPPCTQNVTWSVVRKVRTVTRKQVK 250

Query: 249 LLKSAVEHCAETNARPVQPLNGRQVQLYCP 273
           LL+ AV   A +NARPVQP N R V LY P
Sbjct: 251 LLRVAVHDDANSNARPVQPTNKRIVHLYRP 273

BLAST of Chy4G087460 vs. ExPASy Swiss-Prot
Match: F4JIK2 (Alpha carbonic anhydrase 4 OS=Arabidopsis thaliana OX=3702 GN=ACA4 PE=3 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 8.8e-63
Identity = 122/250 (48.80%), Postives = 162/250 (64.80%), Query Frame = 0

Query: 27  LSHQPGEVCE---FDYIGGSNKGPTKWGAIKGEWSKCNLGKMQSPIDLSTNTINVVNSLG 86
           +SH   EV +   F Y   + KGP  WG I   W  CN G+ QSPIDL+   +++++   
Sbjct: 23  ISHAHSEVDDETPFTYEQKTEKGPEGWGKINPHWKVCNTGRYQSPIDLTNERVSLIHD-Q 82

Query: 87  TLQRKYNPFSAILKNRGHDISVAWEGNVGSIEINGTKYLLKQIHWHSPSEHTLNGRRYDL 146
              R+Y P  A++ NRGHDI V+W+G+ G + I  T + L Q HWHSPSEHT+NG RYDL
Sbjct: 83  AWTRQYKPAPAVITNRGHDIMVSWKGDAGKMTIRKTDFNLVQCHWHSPSEHTVNGTRYDL 142

Query: 147 ELHVVHESLNPNAKYAVVSYLYEIGLPDFFLSKVSRGIKELSTGKKEIKLGSINPNEIRN 206
           ELH+VH S     + AV+  LY++G P+ FL+K+  GIK  + G KEI LG I+P EIR 
Sbjct: 143 ELHMVHTS--ARGRTAVIGVLYKLGEPNEFLTKLLNGIK--AVGNKEINLGMIDPREIRF 202

Query: 207 AGGINYYRYIGSLTVPPCTEGVVWSINKQIGTVSQEQVMLLKSAVEHCAETNARPVQPLN 266
                +YRYIGSLTVPPCTEGV+W++ K++ T+S EQ+  L+ AV+   ETN+RPVQ   
Sbjct: 203 QTR-KFYRYIGSLTVPPCTEGVIWTVVKRVNTISMEQITALRQAVDDGFETNSRPVQDSK 262

Query: 267 GRQVQLYCPN 274
           GR V  Y PN
Sbjct: 263 GRSVWFYDPN 266

BLAST of Chy4G087460 vs. ExPASy Swiss-Prot
Match: Q9FM99 (Alpha carbonic anhydrase 8 OS=Arabidopsis thaliana OX=3702 GN=ACA8 PE=3 SV=3)

HSP 1 Score: 235.0 bits (598), Expect = 1.1e-60
Identity = 116/240 (48.33%), Postives = 164/240 (68.33%), Query Frame = 0

Query: 36  EFDYIGGSNKGPTKWGAIKGEWSKCNLGKMQSPIDLSTNTINVVNSLGTLQRKYNPFSAI 95
           EF Y    NKGP KWG +  EW  C +GKMQSPIDL    + V N  G L+ +Y P +  
Sbjct: 139 EFSYETKGNKGPAKWGTLDAEWKMCGIGKMQSPIDLRDKNVVVSNKFGLLRSQYLPSNTT 198

Query: 96  LKNRGHDISVAWE-GNVG-SIEINGTKYLLKQIHWHSPSEHTLNGRRYDLELHVVHESLN 155
           +KNRGHDI + ++ GN G  + I GT+Y L+Q+HWHSPSEHT+NG+R+ LE H+VHES  
Sbjct: 199 IKNRGHDIMLKFKGGNKGIGVTIRGTRYQLQQLHWHSPSEHTINGKRFALEEHLVHES-- 258

Query: 156 PNAKYAVVSYLYEIGLPDFFLSKVSRGIKELS-TGKKEIKLGSINPNEIRNAGGINYYRY 215
            + +YAVV++LY +G  D FL  + + +K+++ T   E  +G I+P ++ +    +YYRY
Sbjct: 259 KDKRYAVVAFLYNLGASDPFLFSLEKQLKKITDTHASEEHVGIIDPKKL-SFESKHYYRY 318

Query: 216 IGSLTVPPCTEGVVWSINKQIGTVSQEQVMLLKSAVEHCAETNARPVQPLNGRQVQLYCP 273
            GSLT PPC+E V+WS++K+I TVS +QV LL+ AV   +++NARP+Q +N R+V LY P
Sbjct: 319 SGSLTAPPCSENVIWSVSKEIRTVSSKQVKLLRVAVHDASDSNARPLQAVNKRKVYLYKP 375

BLAST of Chy4G087460 vs. ExPASy Swiss-Prot
Match: Q84UV8 (Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3 OS=Nicotiana langsdorffii x Nicotiana sanderae OX=164110 GN=NEC3 PE=1 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 2.9e-58
Identity = 124/258 (48.06%), Postives = 169/258 (65.50%), Query Frame = 0

Query: 16  ILTSTLLWASVLSHQPGEV---CEFDYIGGSNKGPTKWGAIKGEWSKCNLGKMQSPIDLS 75
           +  S L  +SV   + GEV    EF Y   S  GP  WG I+ +W +C+ GK+QSPID+ 
Sbjct: 10  LFISFLFLSSVFLARSGEVDDESEFSYDEKSENGPANWGNIRPDWKECS-GKLQSPIDIF 69

Query: 76  TNTINVVNSLGTLQRKYNPFSAILKNRGHDISVAWEGNVGSIEINGTKYLLKQIHWHSPS 135
                VV++L  LQ+ Y P +A L NRGHDI +  +   G ++IN T+Y LKQ+HWH+PS
Sbjct: 70  DLRAEVVSNLRILQKDYKPSNATLLNRGHDIMLRLDDG-GYLKINETQYQLKQLHWHTPS 129

Query: 136 EHTLNGRRYDLELHVVHESLNPNAKYAVVSYLYEIGL-PDFFLSKVSRGIKELSTGKKEI 195
           EHT+NG R++LE H+VHES   N K+ V+  +YEIGL PD FLS +   +K +   KK I
Sbjct: 130 EHTINGERFNLEAHLVHES--NNGKFVVIGIVYEIGLWPDPFLSMIENDLK-VPANKKGI 189

Query: 196 K--LGSINPNEIRNAGGINYYRYIGSLTVPPCTEGVVWSINKQIGTVSQEQVMLLKSAVE 255
           +  +G I+PN+I+   G  Y+RYIGSLT PPCTEGVVW I++++ TV++ Q+ LL+ AV 
Sbjct: 190 ERGIGIIDPNQIK-LDGKKYFRYIGSLTTPPCTEGVVWIIDRKVKTVTRRQIKLLQEAVH 249

Query: 256 HCAETNARPVQPLNGRQV 268
              ETNARP QP N R +
Sbjct: 250 DGFETNARPTQPENERYI 261

BLAST of Chy4G087460 vs. ExPASy Swiss-Prot
Match: A7MAQ2 (Dioscorin dioA3 OS=Dioscorea japonica OX=4673 GN=dioA3 PE=1 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 1.9e-57
Identity = 121/259 (46.72%), Postives = 160/259 (61.78%), Query Frame = 0

Query: 10  SLAYLTILTSTLLWASVLSHQPGEVCEFDYIGGSNKGPTKWGAIKGEWSKCNLGKMQSPI 69
           +L +L +L+S L   S LS       EF YI G+  GP  WG +K EW  C  G  QSPI
Sbjct: 5   TLLHLLLLSSLLF--SCLSCLTNVEDEFSYIEGNPNGPENWGNLKPEWETCGKGMEQSPI 64

Query: 70  DLSTNTINVVNSLGTLQRKYNPFSAILKNRGHDISVAWEGNVGSIEINGTKYLLKQIHWH 129
            L  N +    +LG L+R Y    A L+N GHD+ V ++GN GS+ IN  +Y LK+IH+H
Sbjct: 65  QLRDNRVIFDQTLGKLRRNYRAVDARLRNSGHDVLVDFKGNAGSLSINRVEYQLKRIHFH 124

Query: 130 SPSEHTLNGRRYDLELHVVHESLNPNAKYAVVSYLYEIGLPDFFLSKVSRGIKELSTGKK 189
           SPSEH +NG R+DLE  +VHES   + K AVVS L+  G  D FLS +   IK+ S  +K
Sbjct: 125 SPSEHEMNGERFDLEAQLVHES--QDQKRAVVSILFRFGRADPFLSDLEDFIKQFSNSQK 184

Query: 190 -EIKLGSINPNEIRNAGGINYYRYIGSLTVPPCTEGVVWSINKQIGTVSQEQVMLLKSAV 249
            EI  G ++PN+++      YYRY+GS T PPCTEG+ W++ +++ TVS  QV+LLK AV
Sbjct: 185 NEINAGVVDPNQLQ-IDDSAYYRYMGSFTAPPCTEGISWTVMRKVATVSPRQVLLLKQAV 244

Query: 250 EHCAETNARPVQPLNGRQV 268
              A  NARP+QP N R V
Sbjct: 245 NENAINNARPLQPTNFRSV 258

BLAST of Chy4G087460 vs. ExPASy TrEMBL
Match: A0A0A0LDR6 (Carbonic anhydrase OS=Cucumis sativus OX=3659 GN=Csa_3G742870 PE=3 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 5.8e-142
Identity = 250/279 (89.61%), Postives = 262/279 (93.91%), Query Frame = 0

Query: 2   LAVSQRTFSLAYLTILTSTLLWASVLSHQPGEVCEFDYIGGSNKGPTKWGAIKGEWSKCN 61
           +A++QRTFSLA+L ILTSTLLWASVLSHQPGEVCEFDYIGGSNKGPTKWGAIK EWSKCN
Sbjct: 1   MALNQRTFSLAFLIILTSTLLWASVLSHQPGEVCEFDYIGGSNKGPTKWGAIKEEWSKCN 60

Query: 62  LGKMQSPIDLSTNTINVVNSLGTLQRKYNPFSAILKNRGHDISVAWEGNVGSIEINGTKY 121
            GKMQSPIDLSTNTINVVNSLG L R+YNPFSA L+NRGHDISVAWEGNVGSIEIN TKY
Sbjct: 61  NGKMQSPIDLSTNTINVVNSLGPLNREYNPFSATLRNRGHDISVAWEGNVGSIEINWTKY 120

Query: 122 LLKQIHWHSPSEHTLNGRRYDLELHVVHESLNPNAKYAVVSYLYEIGLPDFFLSKVSRGI 181
           LLKQIHWHSPSEHTLNGRRYDLELHVVHESLN NAKYAVVS+LYEIG PD FLSKVS GI
Sbjct: 121 LLKQIHWHSPSEHTLNGRRYDLELHVVHESLNSNAKYAVVSHLYEIGPPDAFLSKVSGGI 180

Query: 182 KELSTGKKEIKLGSINPNEIRNAGGINYYRYIGSLTVPPCTEGVVWSINKQIGTVSQEQV 241
           KELSTGKKEIKLGSINP+EIRNA GINYYRYIGSLT PPCTEGVVWSINKQIGTVSQEQV
Sbjct: 181 KELSTGKKEIKLGSINPDEIRNASGINYYRYIGSLTAPPCTEGVVWSINKQIGTVSQEQV 240

Query: 242 MLLKSAVEHCAETNARPVQPLNGRQVQLYCPNPSSIPMP 281
           MLL+S VEHCAETNARPVQPLNGR VQLY  +PSS+P+P
Sbjct: 241 MLLRSTVEHCAETNARPVQPLNGRHVQLYSQDPSSMPIP 279

BLAST of Chy4G087460 vs. ExPASy TrEMBL
Match: A0A5A7TRY7 (Carbonic anhydrase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G003240 PE=3 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 4.2e-124
Identity = 224/278 (80.58%), Postives = 245/278 (88.13%), Query Frame = 0

Query: 1   MLAVSQRTFSLAYLTILTSTLLWASVLSHQPGEVCEFDYIGGSNKGPTKWGAIKGEWSKC 60
           ML +SQ +F+LA+LT LTSTLLWASV+SHQ  E CEFDYIGGSNKGPTKWGAIK EW+ C
Sbjct: 1   MLRLSQSSFTLAFLTFLTSTLLWASVISHQLQEECEFDYIGGSNKGPTKWGAIKREWANC 60

Query: 61  NLGKMQSPIDLSTNTINVVNSLGTLQRKYNPFSAILKNRGHDISVAWEGNVGSIEINGTK 120
           N GKMQSPIDLST TI VVNS+G L ++Y PF AILKNRGHD+SV WEGN GSIEINGT+
Sbjct: 61  NYGKMQSPIDLSTKTIQVVNSMGQLNKRYIPFGAILKNRGHDVSVGWEGNAGSIEINGTQ 120

Query: 121 YLLKQIHWHSPSEHTLNGRRYDLELHVVHESLNPNAKY--AVVSYLYEIGLPDFFLSKVS 180
           Y LKQ HWHSPSEHT+NG+RYDLELHVVH+S NPNAKY  AVVS+LYEIG PD FLSKVS
Sbjct: 121 YFLKQTHWHSPSEHTINGKRYDLELHVVHQSSNPNAKYPLAVVSFLYEIGPPDSFLSKVS 180

Query: 181 RGIKELSTGKK-EIKLGSINPNEIRNAGGINYYRYIGSLTVPPCTEGVVWSINKQIGTVS 240
           R IKELS GKK ++KLGSINPNEIR  GGINYYRY+GSLTVPPCTEGV+WSINKQIGTVS
Sbjct: 181 REIKELSHGKKNQMKLGSINPNEIRR-GGINYYRYVGSLTVPPCTEGVIWSINKQIGTVS 240

Query: 241 QEQVMLLKSAVEHCAETNARPVQPLNGRQ-VQLYCPNP 275
           QEQVMLL+S VEHCAE NARP+QPLNGRQ VQLY PNP
Sbjct: 241 QEQVMLLRSVVEHCAEKNARPIQPLNGRQVVQLYDPNP 277

BLAST of Chy4G087460 vs. ExPASy TrEMBL
Match: A0A1S3B8C9 (Carbonic anhydrase OS=Cucumis melo OX=3656 GN=LOC103487135 PE=3 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 4.2e-124
Identity = 224/278 (80.58%), Postives = 245/278 (88.13%), Query Frame = 0

Query: 1   MLAVSQRTFSLAYLTILTSTLLWASVLSHQPGEVCEFDYIGGSNKGPTKWGAIKGEWSKC 60
           ML +SQ +F+LA+LT LTSTLLWASV+SHQ  E CEFDYIGGSNKGPTKWGAIK EW+ C
Sbjct: 1   MLRLSQSSFTLAFLTFLTSTLLWASVISHQLQEECEFDYIGGSNKGPTKWGAIKREWANC 60

Query: 61  NLGKMQSPIDLSTNTINVVNSLGTLQRKYNPFSAILKNRGHDISVAWEGNVGSIEINGTK 120
           N GKMQSPIDLST TI VVNS+G L ++Y PF AILKNRGHD+SV WEGN GSIEINGT+
Sbjct: 61  NYGKMQSPIDLSTKTIQVVNSMGQLNKRYIPFGAILKNRGHDVSVGWEGNAGSIEINGTQ 120

Query: 121 YLLKQIHWHSPSEHTLNGRRYDLELHVVHESLNPNAKY--AVVSYLYEIGLPDFFLSKVS 180
           Y LKQ HWHSPSEHT+NG+RYDLELHVVH+S NPNAKY  AVVS+LYEIG PD FLSKVS
Sbjct: 121 YFLKQTHWHSPSEHTINGKRYDLELHVVHQSSNPNAKYPLAVVSFLYEIGPPDSFLSKVS 180

Query: 181 RGIKELSTGKK-EIKLGSINPNEIRNAGGINYYRYIGSLTVPPCTEGVVWSINKQIGTVS 240
           R IKELS GKK ++KLGSINPNEIR  GGINYYRY+GSLTVPPCTEGV+WSINKQIGTVS
Sbjct: 181 REIKELSHGKKDQMKLGSINPNEIRR-GGINYYRYVGSLTVPPCTEGVIWSINKQIGTVS 240

Query: 241 QEQVMLLKSAVEHCAETNARPVQPLNGRQ-VQLYCPNP 275
           QEQVMLL+S VEHCAE NARP+QPLNGRQ VQLY PNP
Sbjct: 241 QEQVMLLRSVVEHCAEKNARPIQPLNGRQVVQLYDPNP 277

BLAST of Chy4G087460 vs. ExPASy TrEMBL
Match: A0A5D3DNH9 (Carbonic anhydrase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002870 PE=3 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 9.3e-124
Identity = 223/278 (80.22%), Postives = 245/278 (88.13%), Query Frame = 0

Query: 1   MLAVSQRTFSLAYLTILTSTLLWASVLSHQPGEVCEFDYIGGSNKGPTKWGAIKGEWSKC 60
           ML +SQ +F+LA+LT LTSTLLWASV+SHQ  E CEFDYIGGSNKGPTKWGAIK EW+ C
Sbjct: 1   MLRLSQSSFTLAFLTFLTSTLLWASVISHQLQEECEFDYIGGSNKGPTKWGAIKREWANC 60

Query: 61  NLGKMQSPIDLSTNTINVVNSLGTLQRKYNPFSAILKNRGHDISVAWEGNVGSIEINGTK 120
           N GKMQSPIDLST TI VVN++G L ++Y PF AILKNRGHD+SV WEGN GSIEINGT+
Sbjct: 61  NYGKMQSPIDLSTKTIQVVNNMGQLNKRYIPFGAILKNRGHDVSVGWEGNAGSIEINGTQ 120

Query: 121 YLLKQIHWHSPSEHTLNGRRYDLELHVVHESLNPNAKY--AVVSYLYEIGLPDFFLSKVS 180
           Y LKQ HWHSPSEHT+NG+RYDLELHVVH+S NPNAKY  AVVS+LYEIG PD FLSKVS
Sbjct: 121 YFLKQTHWHSPSEHTINGKRYDLELHVVHQSSNPNAKYPLAVVSFLYEIGPPDSFLSKVS 180

Query: 181 RGIKELSTGKK-EIKLGSINPNEIRNAGGINYYRYIGSLTVPPCTEGVVWSINKQIGTVS 240
           R IKELS GKK ++KLGSINPNEIR  GGINYYRY+GSLTVPPCTEGV+WSINKQIGTVS
Sbjct: 181 REIKELSHGKKNQMKLGSINPNEIRR-GGINYYRYVGSLTVPPCTEGVIWSINKQIGTVS 240

Query: 241 QEQVMLLKSAVEHCAETNARPVQPLNGRQ-VQLYCPNP 275
           QEQVMLL+S VEHCAE NARP+QPLNGRQ VQLY PNP
Sbjct: 241 QEQVMLLRSVVEHCAEKNARPIQPLNGRQVVQLYDPNP 277

BLAST of Chy4G087460 vs. ExPASy TrEMBL
Match: A0A0A0LAM4 (Carbonic anhydrase OS=Cucumis sativus OX=3659 GN=Csa_3G742880 PE=3 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 3.9e-90
Identity = 167/253 (66.01%), Postives = 196/253 (77.47%), Query Frame = 0

Query: 25  SVLSHQPGEVCEFDYIGGSNKGPTKWGAIKGEWSKCNLGKMQSPIDLSTNTINVVNSLGT 84
           S ++ +  +  EFDY  GS KGP+KWG IK EW KCN G MQSPIDLS   + VV  LG 
Sbjct: 21  SAIAQEVEDESEFDYRKGSKKGPSKWGDIKREWEKCNNGNMQSPIDLSNKRVKVVKHLGQ 80

Query: 85  LQRKYNPFSAILKNRGHDISVAWEGNVGSIEINGTKYLLKQIHWHSPSEHTLNGRRYDLE 144
           L+ KY P  AI+KNRGHDISV WE + GSIEINGT YLL+Q HWHSPSEHTLNGRRYDLE
Sbjct: 81  LKNKYKPSIAIIKNRGHDISVKWEEDAGSIEINGTDYLLQQAHWHSPSEHTLNGRRYDLE 140

Query: 145 LHVVHESLNPNAKY--AVVSYLYEIGLPDFFLSKVSRGIKELSTGKKEIKLGSINPNEIR 204
           +H+VH+S NPNAKY  AVV + Y+IG PD FLSK++R IK L+  KKEIK G INP  I+
Sbjct: 141 VHLVHQSSNPNAKYPIAVVGFFYKIGRPDSFLSKLNRKIKALNE-KKEIKAGIINPRWIK 200

Query: 205 NAGGINYYRYIGSLTVPPCTEGVVWSINKQIGTVSQEQVMLLKSAVEHCAETNARPVQPL 264
           N GG+ YYRYIGSLTVPPCTEGV+W+I K+IGTVS++QV LL+SAV   AE NARP+QP 
Sbjct: 201 N-GGMKYYRYIGSLTVPPCTEGVIWNIKKKIGTVSRKQVKLLRSAVHDSAEKNARPIQPH 260

Query: 265 NGRQVQLYCPNPS 276
           NGR + LY PNP+
Sbjct: 261 NGRHIDLYDPNPT 271

BLAST of Chy4G087460 vs. NCBI nr
Match: XP_011652843.1 (alpha carbonic anhydrase 7 [Cucumis sativus] >KGN58999.1 hypothetical protein Csa_001951 [Cucumis sativus])

HSP 1 Score: 508 bits (1307), Expect = 1.91e-180
Identity = 250/279 (89.61%), Postives = 262/279 (93.91%), Query Frame = 0

Query: 2   LAVSQRTFSLAYLTILTSTLLWASVLSHQPGEVCEFDYIGGSNKGPTKWGAIKGEWSKCN 61
           +A++QRTFSLA+L ILTSTLLWASVLSHQPGEVCEFDYIGGSNKGPTKWGAIK EWSKCN
Sbjct: 1   MALNQRTFSLAFLIILTSTLLWASVLSHQPGEVCEFDYIGGSNKGPTKWGAIKEEWSKCN 60

Query: 62  LGKMQSPIDLSTNTINVVNSLGTLQRKYNPFSAILKNRGHDISVAWEGNVGSIEINGTKY 121
            GKMQSPIDLSTNTINVVNSLG L R+YNPFSA L+NRGHDISVAWEGNVGSIEIN TKY
Sbjct: 61  NGKMQSPIDLSTNTINVVNSLGPLNREYNPFSATLRNRGHDISVAWEGNVGSIEINWTKY 120

Query: 122 LLKQIHWHSPSEHTLNGRRYDLELHVVHESLNPNAKYAVVSYLYEIGLPDFFLSKVSRGI 181
           LLKQIHWHSPSEHTLNGRRYDLELHVVHESLN NAKYAVVS+LYEIG PD FLSKVS GI
Sbjct: 121 LLKQIHWHSPSEHTLNGRRYDLELHVVHESLNSNAKYAVVSHLYEIGPPDAFLSKVSGGI 180

Query: 182 KELSTGKKEIKLGSINPNEIRNAGGINYYRYIGSLTVPPCTEGVVWSINKQIGTVSQEQV 241
           KELSTGKKEIKLGSINP+EIRNA GINYYRYIGSLT PPCTEGVVWSINKQIGTVSQEQV
Sbjct: 181 KELSTGKKEIKLGSINPDEIRNASGINYYRYIGSLTAPPCTEGVVWSINKQIGTVSQEQV 240

Query: 242 MLLKSAVEHCAETNARPVQPLNGRQVQLYCPNPSSIPMP 280
           MLL+S VEHCAETNARPVQPLNGR VQLY  +PSS+P+P
Sbjct: 241 MLLRSTVEHCAETNARPVQPLNGRHVQLYSQDPSSMPIP 279

BLAST of Chy4G087460 vs. NCBI nr
Match: KAA0043979.1 (alpha carbonic anhydrase 7-like [Cucumis melo var. makuwa])

HSP 1 Score: 449 bits (1154), Expect = 3.50e-157
Identity = 224/278 (80.58%), Postives = 245/278 (88.13%), Query Frame = 0

Query: 1   MLAVSQRTFSLAYLTILTSTLLWASVLSHQPGEVCEFDYIGGSNKGPTKWGAIKGEWSKC 60
           ML +SQ +F+LA+LT LTSTLLWASV+SHQ  E CEFDYIGGSNKGPTKWGAIK EW+ C
Sbjct: 1   MLRLSQSSFTLAFLTFLTSTLLWASVISHQLQEECEFDYIGGSNKGPTKWGAIKREWANC 60

Query: 61  NLGKMQSPIDLSTNTINVVNSLGTLQRKYNPFSAILKNRGHDISVAWEGNVGSIEINGTK 120
           N GKMQSPIDLST TI VVNS+G L ++Y PF AILKNRGHD+SV WEGN GSIEINGT+
Sbjct: 61  NYGKMQSPIDLSTKTIQVVNSMGQLNKRYIPFGAILKNRGHDVSVGWEGNAGSIEINGTQ 120

Query: 121 YLLKQIHWHSPSEHTLNGRRYDLELHVVHESLNPNAKY--AVVSYLYEIGLPDFFLSKVS 180
           Y LKQ HWHSPSEHT+NG+RYDLELHVVH+S NPNAKY  AVVS+LYEIG PD FLSKVS
Sbjct: 121 YFLKQTHWHSPSEHTINGKRYDLELHVVHQSSNPNAKYPLAVVSFLYEIGPPDSFLSKVS 180

Query: 181 RGIKELSTGKK-EIKLGSINPNEIRNAGGINYYRYIGSLTVPPCTEGVVWSINKQIGTVS 240
           R IKELS GKK ++KLGSINPNEIR  GGINYYRY+GSLTVPPCTEGV+WSINKQIGTVS
Sbjct: 181 REIKELSHGKKNQMKLGSINPNEIRR-GGINYYRYVGSLTVPPCTEGVIWSINKQIGTVS 240

Query: 241 QEQVMLLKSAVEHCAETNARPVQPLNGRQV-QLYCPNP 274
           QEQVMLL+S VEHCAE NARP+QPLNGRQV QLY PNP
Sbjct: 241 QEQVMLLRSVVEHCAEKNARPIQPLNGRQVVQLYDPNP 277

BLAST of Chy4G087460 vs. NCBI nr
Match: XP_008443579.1 (PREDICTED: alpha carbonic anhydrase 7-like [Cucumis melo])

HSP 1 Score: 449 bits (1154), Expect = 3.50e-157
Identity = 224/278 (80.58%), Postives = 245/278 (88.13%), Query Frame = 0

Query: 1   MLAVSQRTFSLAYLTILTSTLLWASVLSHQPGEVCEFDYIGGSNKGPTKWGAIKGEWSKC 60
           ML +SQ +F+LA+LT LTSTLLWASV+SHQ  E CEFDYIGGSNKGPTKWGAIK EW+ C
Sbjct: 1   MLRLSQSSFTLAFLTFLTSTLLWASVISHQLQEECEFDYIGGSNKGPTKWGAIKREWANC 60

Query: 61  NLGKMQSPIDLSTNTINVVNSLGTLQRKYNPFSAILKNRGHDISVAWEGNVGSIEINGTK 120
           N GKMQSPIDLST TI VVNS+G L ++Y PF AILKNRGHD+SV WEGN GSIEINGT+
Sbjct: 61  NYGKMQSPIDLSTKTIQVVNSMGQLNKRYIPFGAILKNRGHDVSVGWEGNAGSIEINGTQ 120

Query: 121 YLLKQIHWHSPSEHTLNGRRYDLELHVVHESLNPNAKY--AVVSYLYEIGLPDFFLSKVS 180
           Y LKQ HWHSPSEHT+NG+RYDLELHVVH+S NPNAKY  AVVS+LYEIG PD FLSKVS
Sbjct: 121 YFLKQTHWHSPSEHTINGKRYDLELHVVHQSSNPNAKYPLAVVSFLYEIGPPDSFLSKVS 180

Query: 181 RGIKELSTGKKE-IKLGSINPNEIRNAGGINYYRYIGSLTVPPCTEGVVWSINKQIGTVS 240
           R IKELS GKK+ +KLGSINPNEIR  GGINYYRY+GSLTVPPCTEGV+WSINKQIGTVS
Sbjct: 181 REIKELSHGKKDQMKLGSINPNEIRR-GGINYYRYVGSLTVPPCTEGVIWSINKQIGTVS 240

Query: 241 QEQVMLLKSAVEHCAETNARPVQPLNGRQV-QLYCPNP 274
           QEQVMLL+S VEHCAE NARP+QPLNGRQV QLY PNP
Sbjct: 241 QEQVMLLRSVVEHCAEKNARPIQPLNGRQVVQLYDPNP 277

BLAST of Chy4G087460 vs. NCBI nr
Match: TYK25161.1 (alpha carbonic anhydrase 7-like [Cucumis melo var. makuwa])

HSP 1 Score: 447 bits (1151), Expect = 1.00e-156
Identity = 223/278 (80.22%), Postives = 245/278 (88.13%), Query Frame = 0

Query: 1   MLAVSQRTFSLAYLTILTSTLLWASVLSHQPGEVCEFDYIGGSNKGPTKWGAIKGEWSKC 60
           ML +SQ +F+LA+LT LTSTLLWASV+SHQ  E CEFDYIGGSNKGPTKWGAIK EW+ C
Sbjct: 1   MLRLSQSSFTLAFLTFLTSTLLWASVISHQLQEECEFDYIGGSNKGPTKWGAIKREWANC 60

Query: 61  NLGKMQSPIDLSTNTINVVNSLGTLQRKYNPFSAILKNRGHDISVAWEGNVGSIEINGTK 120
           N GKMQSPIDLST TI VVN++G L ++Y PF AILKNRGHD+SV WEGN GSIEINGT+
Sbjct: 61  NYGKMQSPIDLSTKTIQVVNNMGQLNKRYIPFGAILKNRGHDVSVGWEGNAGSIEINGTQ 120

Query: 121 YLLKQIHWHSPSEHTLNGRRYDLELHVVHESLNPNAKY--AVVSYLYEIGLPDFFLSKVS 180
           Y LKQ HWHSPSEHT+NG+RYDLELHVVH+S NPNAKY  AVVS+LYEIG PD FLSKVS
Sbjct: 121 YFLKQTHWHSPSEHTINGKRYDLELHVVHQSSNPNAKYPLAVVSFLYEIGPPDSFLSKVS 180

Query: 181 RGIKELSTGKK-EIKLGSINPNEIRNAGGINYYRYIGSLTVPPCTEGVVWSINKQIGTVS 240
           R IKELS GKK ++KLGSINPNEIR  GGINYYRY+GSLTVPPCTEGV+WSINKQIGTVS
Sbjct: 181 REIKELSHGKKNQMKLGSINPNEIRR-GGINYYRYVGSLTVPPCTEGVIWSINKQIGTVS 240

Query: 241 QEQVMLLKSAVEHCAETNARPVQPLNGRQV-QLYCPNP 274
           QEQVMLL+S VEHCAE NARP+QPLNGRQV QLY PNP
Sbjct: 241 QEQVMLLRSVVEHCAEKNARPIQPLNGRQVVQLYDPNP 277

BLAST of Chy4G087460 vs. NCBI nr
Match: XP_038905903.1 (alpha carbonic anhydrase 7-like [Benincasa hispida])

HSP 1 Score: 343 bits (879), Expect = 2.19e-115
Identity = 178/280 (63.57%), Postives = 210/280 (75.00%), Query Frame = 0

Query: 1   MLAVSQRTFSLAYLTILTSTLLWASVLSHQPGEVCEFDYIGGSNKGPTKWGAIKGEWSKC 60
           ML ++QR  +LA+       L+  S ++ +  +  EF+YI GS KGP +WG IK EWSKC
Sbjct: 1   MLTLNQRASALAFFI-----LILTSAIAQEVEDESEFNYIRGSEKGPKRWGEIKREWSKC 60

Query: 61  NLGKMQSPIDLSTNTINVVNSLGTLQRKYNPFSAILKNRGHDISVAWEGNVGSIEINGTK 120
           N G MQSPIDLST  + VV +LG L++KY P  AI+KNRGHDISV WE N G IEINGT 
Sbjct: 61  NNGDMQSPIDLSTKRVKVVKNLGQLKKKYKPSIAIVKNRGHDISVKWEENAGLIEINGTD 120

Query: 121 YLLKQIHWHSPSEHTLNGRRYDLELHVVHESLNPNAKY--AVVSYLYEIGLPDFFLSKVS 180
           YLL+Q HWHSPSEHTLNGRRYDLELH+VH+S NPNAKY  AVV   Y IG PD FLSK+S
Sbjct: 121 YLLQQAHWHSPSEHTLNGRRYDLELHLVHQSSNPNAKYPIAVVGIFYMIGRPDSFLSKMS 180

Query: 181 RGIKELSTGKKEIKLGSINPNEIRNAGGINYYRYIGSLTVPPCTEGVVWSINKQIGTVSQ 240
           R IK L+   KE+K GSI+P  I+  GG  YYRY+GSLTVPPCTEGV+W+INK+IGTVS+
Sbjct: 181 RKIKALNE-VKEMKAGSIDPRWIK-MGGKKYYRYMGSLTVPPCTEGVIWNINKKIGTVSR 240

Query: 241 EQVMLLKSAVEHCAETNARPVQPLNGRQVQLYCPNPSSIP 278
           EQV LL+SAV   AE NARP+QP NGRQV LY PNP+  P
Sbjct: 241 EQVKLLRSAVHDSAEKNARPIQPHNGRQVDLYNPNPTQTP 273

BLAST of Chy4G087460 vs. TAIR 10
Match: AT1G08080.1 (alpha carbonic anhydrase 7 )

HSP 1 Score: 251.5 bits (641), Expect = 7.9e-67
Identity = 134/270 (49.63%), Postives = 176/270 (65.19%), Query Frame = 0

Query: 9   FSLAYLTILTSTLLWASVLSHQPGEV---CEFDYIGGSNKGPTKWGAIKGEWSKCNLGKM 68
           F +A  +I T   + ++  SH  GEV    EF+Y     KGP +WG +K EW  C  G+M
Sbjct: 11  FFVALFSIFTIVSISSAASSH--GEVEDEREFNYKKNDEKGPERWGELKPEWEMCGKGEM 70

Query: 69  QSPIDLSTNTINVVNSLGTLQRKYNPFSAILKNRGHDISVAWEGNVGSIEINGTKYLLKQ 128
           QSPIDL    +N+V+ LG L R YNP +A LKNRGHDI + +E   G+I+ING +Y L+Q
Sbjct: 71  QSPIDLMNERVNIVSHLGRLNRDYNPSNATLKNRGHDIMLKFEDGAGTIKINGFEYELQQ 130

Query: 129 IHWHSPSEHTLNGRRYDLELHVVHESLNPNAKYAVVSYLYEIGLPDFF---LSKVSRGIK 188
           +HWHSPSEHT+NGRR+ LELH+VHE    N + AVV+ LY+IG  D F   L K   GI 
Sbjct: 131 LHWHSPSEHTINGRRFALELHMVHE--GRNRRMAVVTVLYKIGRADTFIRSLEKELEGIA 190

Query: 189 ELSTGKKEIKLGSINPNEIRNAGGINYYRYIGSLTVPPCTEGVVWSINKQIGTVSQEQVM 248
           E+   +K +  G I+P +I+  G   YYRY GSLT PPCT+ V WS+ +++ TV+++QV 
Sbjct: 191 EMEEAEKNV--GMIDPTKIK-IGSRKYYRYTGSLTTPPCTQNVTWSVVRKVRTVTRKQVK 250

Query: 249 LLKSAVEHCAETNARPVQPLNGRQVQLYCP 273
           LL+ AV   A +NARPVQP N R V LY P
Sbjct: 251 LLRVAVHDDANSNARPVQPTNKRIVHLYRP 273

BLAST of Chy4G087460 vs. TAIR 10
Match: AT4G20990.1 (alpha carbonic anhydrase 4 )

HSP 1 Score: 241.9 bits (616), Expect = 6.2e-64
Identity = 122/250 (48.80%), Postives = 162/250 (64.80%), Query Frame = 0

Query: 27  LSHQPGEVCE---FDYIGGSNKGPTKWGAIKGEWSKCNLGKMQSPIDLSTNTINVVNSLG 86
           +SH   EV +   F Y   + KGP  WG I   W  CN G+ QSPIDL+   +++++   
Sbjct: 23  ISHAHSEVDDETPFTYEQKTEKGPEGWGKINPHWKVCNTGRYQSPIDLTNERVSLIHD-Q 82

Query: 87  TLQRKYNPFSAILKNRGHDISVAWEGNVGSIEINGTKYLLKQIHWHSPSEHTLNGRRYDL 146
              R+Y P  A++ NRGHDI V+W+G+ G + I  T + L Q HWHSPSEHT+NG RYDL
Sbjct: 83  AWTRQYKPAPAVITNRGHDIMVSWKGDAGKMTIRKTDFNLVQCHWHSPSEHTVNGTRYDL 142

Query: 147 ELHVVHESLNPNAKYAVVSYLYEIGLPDFFLSKVSRGIKELSTGKKEIKLGSINPNEIRN 206
           ELH+VH S     + AV+  LY++G P+ FL+K+  GIK  + G KEI LG I+P EIR 
Sbjct: 143 ELHMVHTS--ARGRTAVIGVLYKLGEPNEFLTKLLNGIK--AVGNKEINLGMIDPREIRF 202

Query: 207 AGGINYYRYIGSLTVPPCTEGVVWSINKQIGTVSQEQVMLLKSAVEHCAETNARPVQPLN 266
                +YRYIGSLTVPPCTEGV+W++ K++ T+S EQ+  L+ AV+   ETN+RPVQ   
Sbjct: 203 QTR-KFYRYIGSLTVPPCTEGVIWTVVKRVNTISMEQITALRQAVDDGFETNSRPVQDSK 262

Query: 267 GRQVQLYCPN 274
           GR V  Y PN
Sbjct: 263 GRSVWFYDPN 266

BLAST of Chy4G087460 vs. TAIR 10
Match: AT1G08065.1 (alpha carbonic anhydrase 5 )

HSP 1 Score: 219.9 bits (559), Expect = 2.5e-57
Identity = 112/263 (42.59%), Postives = 174/263 (66.16%), Query Frame = 0

Query: 13  YLTILTSTLLWASVLSHQPGEVCEFDYIGGSNKGPTKWGAIKGEWSKCNLGKMQSPIDLS 72
           +L  ++ T++ +S    +  +  +F+Y     KGP  WG +K EW+ C  G MQSPIDL+
Sbjct: 11  FLIFISITIVSSSPDHGEVEDETQFNYEKKGEKGPENWGRLKPEWAMCGKGNMQSPIDLT 70

Query: 73  TNTINVVNSLGTLQRKYNPFSAILKNRGHDISVAWE-GNVG-SIEINGTKYLLKQIHWHS 132
              + + ++LG L+ +Y P +A +KNRGHDI + +E GN G  I INGT+Y L+QIHWHS
Sbjct: 71  DKRVLIDHNLGYLRSQYLPSNATIKNRGHDIMMKFEGGNAGLGITINGTEYKLQQIHWHS 130

Query: 133 PSEHTLNGRRYDLELHVVHESLNPNAKYAVVSYLYEIGLPDFFLSKVSRGIKELS-TGKK 192
           PSEHTLNG+R+ LE H+VH+S   + + AVV++ Y++G PD+FL  + R +K ++ T + 
Sbjct: 131 PSEHTLNGKRFVLEEHMVHQS--KDGRNAVVAFFYKLGKPDYFLLTLERYLKRITDTHES 190

Query: 193 EIKLGSINPNEIRNAGGINYYRYIGSLTVPPCTEGVVWSINKQIGTVSQEQVMLLKSAVE 252
           +  +  ++P         +YYR+IGSLT PPC+E V+W+I+K++ TV+ +Q+++L+  V 
Sbjct: 191 QEFVEMVHPRTF-GFESKHYYRFIGSLTTPPCSENVIWTISKEMRTVTLKQLIMLRVTVH 250

Query: 253 HCAETNARPVQPLNGRQVQLYCP 273
             + +NARP+Q  N R V LY P
Sbjct: 251 DQSNSNARPLQRKNERPVALYIP 270

BLAST of Chy4G087460 vs. TAIR 10
Match: AT4G21000.1 (alpha carbonic anhydrase 6 )

HSP 1 Score: 210.3 bits (534), Expect = 2.0e-54
Identity = 102/225 (45.33%), Postives = 150/225 (66.67%), Query Frame = 0

Query: 9   FSLAYLTILTSTLLWASVLSHQPGEVCEFDYIGGSNKGPTKWGAIKGEWSKCNLGKMQSP 68
           F + ++ + +  +L+  V + + G    F Y   + KGP +WG +  +W  C+ GK+QSP
Sbjct: 11  FVVFFIDLFSPNILF--VYAREIGNKPLFTYKQKTEKGPAEWGKLDPQWKVCSTGKIQSP 70

Query: 69  IDLSTNTINVVNSLGTLQRKYNPFSAILKNRGHDISVAWEGNVGSIEINGTKYLLKQIHW 128
           IDL+   +++++    L + Y P SA++++RGHD+ V+W+G+ G I I+ T Y L Q HW
Sbjct: 71  IDLTDERVSLIHD-QALSKHYKPASAVIQSRGHDVMVSWKGDGGKITIHQTDYKLVQCHW 130

Query: 129 HSPSEHTLNGRRYDLELHVVHESLNPNAKYAVVSYLYEIGLPDFFLSKVSRGIKELSTGK 188
           HSPSEHT+NG  YDLELH+VH S   + K  VV  LY++G PD FL+K+  GIK    GK
Sbjct: 131 HSPSEHTINGTSYDLELHMVHTS--ASGKTTVVGVLYKLGEPDEFLTKILNGIK--GVGK 190

Query: 189 KEIKLGSINPNEIRNAGGINYYRYIGSLTVPPCTEGVVWSINKQI 234
           KEI LG ++P +IR     N+YRYIGSLT+PPCTEGV+W++ K++
Sbjct: 191 KEIDLGIVDPRDIRFETN-NFYRYIGSLTIPPCTEGVIWTVQKRV 227

BLAST of Chy4G087460 vs. TAIR 10
Match: AT5G04180.1 (alpha carbonic anhydrase 3 )

HSP 1 Score: 207.2 bits (526), Expect = 1.7e-53
Identity = 119/267 (44.57%), Postives = 154/267 (57.68%), Query Frame = 0

Query: 16  ILTSTLLWASVLSHQPGEVCEFDYIGGSNKGPTKWGAIKGEWSKCNLGKMQSPIDLSTNT 75
           IL  T L  S  S       EF Y  G    P+KW +IK EW  C  GK QSPI+L+   
Sbjct: 5   ILFVTFLALSSSSLADETETEFHYKPGEIADPSKWSSIKAEWKICGTGKRQSPINLTPKI 64

Query: 76  INVV-NSLGTLQRKYNPFSAILKNRGHDISVAWEGNVGSIEINGTKYLLKQIHWHSPSEH 135
             +V NS   LQ  Y P  AILKNRG D+ V WE + G I IN T Y L Q HWH+PSEH
Sbjct: 65  ARIVHNSTEILQTYYKPVEAILKNRGFDMKVKWEDDAGKIVINDTDYKLVQSHWHAPSEH 124

Query: 136 TLNGRRYDLELHVVHESLNPNAKYAVVSYLYEIGLPDFFLSKVSRGIKELSTGKK-EIKL 195
            L+G+R  +ELH+VH+S+      AV+  L+  G P+ F+S++   I +++  +  E+ +
Sbjct: 125 FLDGQRLAMELHMVHKSV--EGHLAVIGVLFREGEPNAFISRIMDKIHKIADVQDGEVSI 184

Query: 196 GSINPNEIRNAGGINYYRYIGSLTVPPCTEGVVWSINKQIGTVSQEQVMLLKSAVEHCAE 255
           G I+P E        +Y Y GSLT PPCTE V+W+I  ++GTVS+EQ+ +L  A     E
Sbjct: 185 GKIDPREF-GWDLTKFYEYRGSLTTPPCTEDVMWTIINKVGTVSREQIDVLTDARRGGYE 244

Query: 256 TNARPVQPLNGRQVQLYCPNPSSIPMP 281
            NARP QPLNGR V L   N  S P P
Sbjct: 245 KNARPAQPLNGRLVYL---NEQSSPSP 265

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8L8171.1e-6549.63Alpha carbonic anhydrase 7 OS=Arabidopsis thaliana OX=3702 GN=ACA7 PE=2 SV=1[more]
F4JIK28.8e-6348.80Alpha carbonic anhydrase 4 OS=Arabidopsis thaliana OX=3702 GN=ACA4 PE=3 SV=1[more]
Q9FM991.1e-6048.33Alpha carbonic anhydrase 8 OS=Arabidopsis thaliana OX=3702 GN=ACA8 PE=3 SV=3[more]
Q84UV82.9e-5848.06Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3 OS... [more]
A7MAQ21.9e-5746.72Dioscorin dioA3 OS=Dioscorea japonica OX=4673 GN=dioA3 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LDR65.8e-14289.61Carbonic anhydrase OS=Cucumis sativus OX=3659 GN=Csa_3G742870 PE=3 SV=1[more]
A0A5A7TRY74.2e-12480.58Carbonic anhydrase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G0... [more]
A0A1S3B8C94.2e-12480.58Carbonic anhydrase OS=Cucumis melo OX=3656 GN=LOC103487135 PE=3 SV=1[more]
A0A5D3DNH99.3e-12480.22Carbonic anhydrase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G0... [more]
A0A0A0LAM43.9e-9066.01Carbonic anhydrase OS=Cucumis sativus OX=3659 GN=Csa_3G742880 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_011652843.11.91e-18089.61alpha carbonic anhydrase 7 [Cucumis sativus] >KGN58999.1 hypothetical protein Cs... [more]
KAA0043979.13.50e-15780.58alpha carbonic anhydrase 7-like [Cucumis melo var. makuwa][more]
XP_008443579.13.50e-15780.58PREDICTED: alpha carbonic anhydrase 7-like [Cucumis melo][more]
TYK25161.11.00e-15680.22alpha carbonic anhydrase 7-like [Cucumis melo var. makuwa][more]
XP_038905903.12.19e-11563.57alpha carbonic anhydrase 7-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G08080.17.9e-6749.63alpha carbonic anhydrase 7 [more]
AT4G20990.16.2e-6448.80alpha carbonic anhydrase 4 [more]
AT1G08065.12.5e-5742.59alpha carbonic anhydrase 5 [more]
AT4G21000.12.0e-5445.33alpha carbonic anhydrase 6 [more]
AT5G04180.11.7e-5344.57alpha carbonic anhydrase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001148Alpha carbonic anhydrase domainSMARTSM01057Carb_anhydrase_2acoord: 37..271
e-value: 1.1E-63
score: 227.6
IPR001148Alpha carbonic anhydrase domainPFAMPF00194Carb_anhydrasecoord: 44..268
e-value: 7.5E-42
score: 143.6
IPR001148Alpha carbonic anhydrase domainPROSITEPS51144ALPHA_CA_2coord: 35..271
score: 44.240723
IPR036398Alpha carbonic anhydrase domain superfamilyGENE3D3.10.200.10Alpha carbonic anhydrasecoord: 34..273
e-value: 2.9E-69
score: 235.2
IPR036398Alpha carbonic anhydrase domain superfamilySUPERFAMILY51069Carbonic anhydrasecoord: 36..267
IPR023561Carbonic anhydrase, alpha-classPANTHERPTHR18952CARBONIC ANHYDRASEcoord: 12..274
NoneNo IPR availablePANTHERPTHR18952:SF201ALPHA CARBONIC ANHYDRASE 3coord: 12..274
IPR018338Carbonic anhydrase, alpha-class, conserved sitePROSITEPS00162ALPHA_CA_1coord: 132..148
IPR041891Carbonic anhydrase, prokaryotic-likeCDDcd03124alpha_CA_prokaryotic_likecoord: 46..269
e-value: 1.62914E-87
score: 257.97

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy4G087460.1Chy4G087460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006730 one-carbon metabolic process
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0004089 carbonate dehydratase activity
molecular_function GO:0008270 zinc ion binding