Chy4G087380 (gene) Cucumber (hystrix) v1

Overview
NameChy4G087380
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionsplicing factor 3A subunit 2
LocationchrH04: 25889608 .. 25892320 (-)
RNA-Seq ExpressionChy4G087380
SyntenyChy4G087380
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATAGAGAGTGGGGGTCGAAGCCGGGAAGTGGAGGAGCGGCCACTGCACAAAATGAAGCTATCGATCGTAGAGAGCGTCTTCGACGGCTTGCTCTCGAGACCATCGATCTCGCCAAGGATCCTTATTTTATGCGCAATCATCTTGGAAGGTCCTTCCTTTCTCTTGCTCATTCTAGTTCTATCCGTTTTTTTTGGTAATTCTTGAGTTGAGTTTTATTGGTATTTGGGGTGTTTTTGTAGCTATGAGTGTAAGCTTTGCTTGACTCTGCACAACAATGAGGGAAATTACTTGGCGCATACGCAGGGGAAGCGGCATCAGACTAATTTGGCCAAGAGAGCTGCCCGTGAAGCTAAGGAGGCTCCTGCTCAACCTCAACCCCACAAGAGGAAGGTCTCTGTTCGCAAAACAGGCAAGTTTTTGTGCCCTTCTCCTTGTGTATTTATGTCTTCCACGTTAATTGTGACTGAGGGTGAACAGTAAGTTTATTGTTGCTTCTTTGTAATCCTTCTGAAGGGTCGCTGCCAAGTTAATTTTAATTTGCTTCTCGATAGTGAGGATTGATATGAAATGATTTTGGTATAATTCAATTCTTTGCAATTGGATTGGTAATACTCTTAGCTTGACCATTGTATCATAACGACGTAATCGGAACAGCGAGTTTAAGATTTCTGCTTTTAAGTCAAGTCACATTGATATGTATCCAATTTACTTCACGTCGAGTAAGAATTCGCAGTTGGAAATGGGTAGTGTTTTGGGGTAGCTTTGAAATTTGAAGTGTTGGTTGTAATTTACTGGATGTGCTCCTTATTGTTTGTGTTTGTACTTAAAAGTGGAGTAGTGCTAATTAACTTCTTCCCTGACATACTTTCAGTTAAAATTGGAAGGCCCGGTTATCGAGTAACAAAGCAATTTGATTCTGAAACAAAGCAGCGATCTCTCCTTTTTCAGGTATGGCTTTATAGTTCGTTCAACAGGCTTCTTTAGCATCTGTTGAAGATGATTTTCATTGAATTATTACTCAATTGTGTTGTGTCTGATTTGCCTCTCCTCTCATATATTGTTGTTATTCCATGCAGATTGAATATCCTGAAATTGAAGACCTTGCAAAGCCTAGGCATCGGTTTATGTCCTCTTACGAGCAGGTTAACTAAGTTTCACTATTACATGCTTCTGAAAGCACGATATTCCCTTTTATCATTTCTATTTTCTCATACAGCTAATTTTTCATATGTTCAGCGTGTGCAACCTTTTGACAAACGATACCAATATCTTCTGTTTGCAGCCGAACCATATGAGATAATTGCATTCAAGGTATTCTAGACATGATAATCGTAAAATATATTGATCATAGGTTTGGCTTTTTAGCTCCTACTGAGTACTGACAGATCTTTACTTGAATGGATTTTAGGTTCCAAGTACAGAGATAGACAAATCGACTCCAAAATTTTTTTCACACTGGGATCCGGACTCTAAAATGTTCACGGTTTAGCTCTTTTCCTTTCCTTCTTTCTAACTTATATAAATACGAATATTTTTTTTCTTGTTCATGTGACACTCTTAGCTCATTACTTGCAGTTCATCTGTATCTTAGGAAGTTAGGATTATTTACCGCCGACTTTAACTTTTTAGTGAATGAATTAAGTTTCTTTTTAAATCTATTTAGTATTAAACAAATCTATTGTAGTTTGGCTTCCCCGTGAGTAAGTATTAAGATAGAAAATATTGATCGGATAGATTGCATCATGATTCCCTTTTATTGTTACTACTTGTTTGAATTATTTGCATCATGATTCCCTTTTATTGTTACTACTTGTTTGAATTATTTTGGCTTTTGGGCCCCAAGTATCCGTGTATGCATTCTTGACTGGCTGATTGCATGCTTGGGAGTGGTTTTGAAATTGTCACTCTTTTAATCATGTTCGAAGTTTTAAATAAATGAGTAAATTGATTTTGAACAAGGTGTTTTAGGGTGTGATTTTGATAGAACATGACAAATGATTCTTCTCATTTCAACATCACTTTAAAACATATTTTTACGATTGGTTTTGAAATTGTTAATTGCCATTTCAAAATCACCTTCTCCTCGTGAGGCTACACCTTAGTTGTCAGCCCTTCATATCAGTGGTTTTTATTCTTTCATATTTAAAAATATGACACTCTTTCTTTTAGATATGGTTAATGATTTAGCACTTTTAATCTTGGAACCAGCAACCAAAATGGTTACAGTTTACTAGTGTCATTTAGGTTTTGATGATGGACTTTTCTAACGACTATACTGAATATGAAATTTGGCAGTTGCAATTGTATTTCAAATCCAAGCCACAGGAGGCAAACAAGCCACCACCTGTGCCTGCAGCCAATGGAACAGTGCCATCCGGTGCGCCCCCAAGGCCACTGCCGCCACCACCACAAGCTCCACCACCGCCACCACCTCCACCCCAGCAAGGAGCACCACCAAGAGTTCCTCCTCCTCCAATGCCAGGGTCCTTGCCGCCTCCACCTTCTGTAATGGCCAATGGCCCTCCTAGGCCAATGCCTCCTGGTGGGGCTCCGCCCATCCCTCCACCACCTCCCATTGGCAATAACACAATGGCGAATTTCACTCCTGGTACTCAGATGAATAGGCCTCCAATGCCGCCTCCTCCACAGGGCTTCCCAGGTCAGGGTATCCGGCAGCCACCACCACCACCACCACCCAATATGCAATAG

mRNA sequence

ATGGATAGAGAGTGGGGGTCGAAGCCGGGAAGTGGAGGAGCGGCCACTGCACAAAATGAAGCTATCGATCGTAGAGAGCGTCTTCGACGGCTTGCTCTCGAGACCATCGATCTCGCCAAGGATCCTTATTTTATGCGCAATCATCTTGGAAGCTATGAGTGTAAGCTTTGCTTGACTCTGCACAACAATGAGGGAAATTACTTGGCGCATACGCAGGGGAAGCGGCATCAGACTAATTTGGCCAAGAGAGCTGCCCGTGAAGCTAAGGAGGCTCCTGCTCAACCTCAACCCCACAAGAGGAAGGTCTCTGTTCGCAAAACAGTTAAAATTGGAAGGCCCGGTTATCGAGTAACAAAGCAATTTGATTCTGAAACAAAGCAGCGATCTCTCCTTTTTCAGATTGAATATCCTGAAATTGAAGACCTTGCAAAGCCTAGGCATCGGTTTATGTCCTCTTACGAGCAGCGTGTGCAACCTTTTGACAAACGATACCAATATCTTCTGTTTGCAGCCGAACCATATGAGATAATTGCATTCAAGGTTCCAAGTACAGAGATAGACAAATCGACTCCAAAATTTTTTTCACACTGGGATCCGGACTCTAAAATGTTCACGTTGCAATTGTATTTCAAATCCAAGCCACAGGAGGCAAACAAGCCACCACCTGTGCCTGCAGCCAATGGAACAGTGCCATCCGGTGCGCCCCCAAGGCCACTGCCGCCACCACCACAAGCTCCACCACCGCCACCACCTCCACCCCAGCAAGGAGCACCACCAAGAGTTCCTCCTCCTCCAATGCCAGGGTCCTTGCCGCCTCCACCTTCTGTAATGGCCAATGGCCCTCCTAGGCCAATGCCTCCTGGTGGGGCTCCGCCCATCCCTCCACCACCTCCCATTGGCAATAACACAATGGCGAATTTCACTCCTGGTACTCAGATGAATAGGCCTCCAATGCCGCCTCCTCCACAGGGCTTCCCAGGTCAGGGTATCCGGCAGCCACCACCACCACCACCACCCAATATGCAATAG

Coding sequence (CDS)

ATGGATAGAGAGTGGGGGTCGAAGCCGGGAAGTGGAGGAGCGGCCACTGCACAAAATGAAGCTATCGATCGTAGAGAGCGTCTTCGACGGCTTGCTCTCGAGACCATCGATCTCGCCAAGGATCCTTATTTTATGCGCAATCATCTTGGAAGCTATGAGTGTAAGCTTTGCTTGACTCTGCACAACAATGAGGGAAATTACTTGGCGCATACGCAGGGGAAGCGGCATCAGACTAATTTGGCCAAGAGAGCTGCCCGTGAAGCTAAGGAGGCTCCTGCTCAACCTCAACCCCACAAGAGGAAGGTCTCTGTTCGCAAAACAGTTAAAATTGGAAGGCCCGGTTATCGAGTAACAAAGCAATTTGATTCTGAAACAAAGCAGCGATCTCTCCTTTTTCAGATTGAATATCCTGAAATTGAAGACCTTGCAAAGCCTAGGCATCGGTTTATGTCCTCTTACGAGCAGCGTGTGCAACCTTTTGACAAACGATACCAATATCTTCTGTTTGCAGCCGAACCATATGAGATAATTGCATTCAAGGTTCCAAGTACAGAGATAGACAAATCGACTCCAAAATTTTTTTCACACTGGGATCCGGACTCTAAAATGTTCACGTTGCAATTGTATTTCAAATCCAAGCCACAGGAGGCAAACAAGCCACCACCTGTGCCTGCAGCCAATGGAACAGTGCCATCCGGTGCGCCCCCAAGGCCACTGCCGCCACCACCACAAGCTCCACCACCGCCACCACCTCCACCCCAGCAAGGAGCACCACCAAGAGTTCCTCCTCCTCCAATGCCAGGGTCCTTGCCGCCTCCACCTTCTGTAATGGCCAATGGCCCTCCTAGGCCAATGCCTCCTGGTGGGGCTCCGCCCATCCCTCCACCACCTCCCATTGGCAATAACACAATGGCGAATTTCACTCCTGGTACTCAGATGAATAGGCCTCCAATGCCGCCTCCTCCACAGGGCTTCCCAGGTCAGGGTATCCGGCAGCCACCACCACCACCACCACCCAATATGCAATAG

Protein sequence

MDREWGSKPGSGGAATAQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTLHNNEGNYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKTVKIGRPGYRVTKQFDSETKQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAAEPYEIIAFKVPSTEIDKSTPKFFSHWDPDSKMFTLQLYFKSKPQEANKPPPVPAANGTVPSGAPPRPLPPPPQAPPPPPPPPQQGAPPRVPPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPIGNNTMANFTPGTQMNRPPMPPPPQGFPGQGIRQPPPPPPPNMQ*
Homology
BLAST of Chy4G087380 vs. ExPASy Swiss-Prot
Match: Q15428 (Splicing factor 3A subunit 2 OS=Homo sapiens OX=9606 GN=SF3A2 PE=1 SV=2)

HSP 1 Score: 294.7 bits (753), Expect = 1.4e-78
Identity = 204/361 (56.51%), Postives = 236/361 (65.37%), Query Frame = 0

Query: 6   GSKPGSGGAATAQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTLHNNEG 65
           G K GSGG A++     DRRERLR+LALETID+ KDPYFM+NHLGSYECKLCLTLHNNEG
Sbjct: 8   GGKTGSGGVASSSESNRDRRERLRQLALETIDINKDPYFMKNHLGSYECKLCLTLHNNEG 67

Query: 66  NYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKTVKIGRPGYRVTKQFDSET 125
           +YLAHTQGK+HQTNLA+RAA+EAKEAPAQP P K KV V+K VKIGRPGY+VTKQ DSE 
Sbjct: 68  SYLAHTQGKKHQTNLARRAAKEAKEAPAQPAPEKVKVEVKKFVKIGRPGYKVTKQRDSEM 127

Query: 126 KQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAAEPYEIIAFKVPSTE 185
            Q+SLLFQI+YPEI +   PRHRFMS+YEQR++P D+R+QYLL AAEPYE IAFKVPS E
Sbjct: 128 GQQSLLFQIDYPEIAEGIMPRHRFMSAYEQRIEPPDRRWQYLLMAAEPYETIAFKVPSRE 187

Query: 186 IDKSTPKFFSHWDPDSKMFTLQLYFKSKPQEAN----------KPPPVPAANGTVPSGAP 245
           IDK+  KF++HW+ ++K F LQ +FK +   A           K PP P  NG  P    
Sbjct: 188 IDKAEGKFWTHWNRETKQFFLQFHFKMEKPPAPPSLPAGPPGVKRPPPPLMNGLPPRPPL 247

Query: 246 PRPLPPPPQA----PPPPPPPPQQGAPPRVP--PPPMPGSLPPPPSV--MANG--PPRPM 305
           P  LPPPP      PP PP  P    PP  P  PPP PG  PP P V   A+G  PP P 
Sbjct: 248 PESLPPPPPGGLPLPPMPPTGPAPSGPPGPPQLPPPAPGVHPPAPVVHPPASGVHPPAPG 307

Query: 306 PPGGAPPIPPPPPIGNNTMANFTPGTQMNRPPMP---PPPQGF--PGQGIRQPPP---PP 339
               AP + PP P  +   +   P      PP P   PP  G   P  G+  P P   PP
Sbjct: 308 VHPPAPGVHPPAPGVHPPTSGVHPPAPGVHPPAPGVHPPAPGVHPPAPGVHPPAPGVHPP 367

BLAST of Chy4G087380 vs. ExPASy Swiss-Prot
Match: Q6AXT8 (Splicing factor 3A subunit 2 OS=Rattus norvegicus OX=10116 GN=Sf3a2 PE=2 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 6.9e-78
Identity = 200/350 (57.14%), Postives = 239/350 (68.29%), Query Frame = 0

Query: 6   GSKPGSGGAATAQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTLHNNEG 65
           G K GSGG A++     DRRERLR+LALETID+ KDPYFM+NHLGSYECKLCLTLHNNEG
Sbjct: 8   GGKTGSGGVASSSESNRDRRERLRQLALETIDINKDPYFMKNHLGSYECKLCLTLHNNEG 67

Query: 66  NYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKTVKIGRPGYRVTKQFDSET 125
           +YLAHTQGK+HQTNLA+RAA+EAKEAPAQP P K KV V+K VKIGRPGY+VTKQ D+E 
Sbjct: 68  SYLAHTQGKKHQTNLARRAAKEAKEAPAQPAPEKVKVEVKKFVKIGRPGYKVTKQRDTEM 127

Query: 126 KQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAAEPYEIIAFKVPSTE 185
            Q+SLLFQI+YPEI +   PRHRFMS+YEQR++P D+R+QYLL AAEPYE IAFKVPS E
Sbjct: 128 GQQSLLFQIDYPEIAEGVMPRHRFMSAYEQRIEPPDRRWQYLLMAAEPYETIAFKVPSRE 187

Query: 186 IDKSTPKFFSHWDPDSKMFTLQLYFKSKPQEANKPPPVPAANGTVPSGAPPRPLPPPPQA 245
           IDK+  KF++HW+ ++K F LQ +FK +     KPP  P    ++P+G P    PPPP  
Sbjct: 188 IDKAEGKFWTHWNRETKQFFLQFHFKME-----KPPAPP----SLPAGPPGVKRPPPPLM 247

Query: 246 PPPPPPPPQQGAPPRVPPPPMPGSLP----PPPSVMANGPPRP--MPPGGAPPIPPPPPI 305
              PP PP    P  +PPPP PG LP    PP     +GPP P  MPP  AP + PP P+
Sbjct: 248 NGLPPRPP---LPDALPPPP-PGGLPLPPMPPTGPAPSGPPGPPQMPP-PAPGVHPPAPV 307

Query: 306 GNNTMANFTPGTQMNRPPMP---PPPQGF--PGQGIRQPPP---PPPPNM 342
            +   +   P      PP P   PP  G   P  G+  P P   PP P +
Sbjct: 308 VHPPTSGVHPPAPGVHPPAPVVHPPTSGVHPPAPGVHPPAPGVHPPAPGV 343

BLAST of Chy4G087380 vs. ExPASy Swiss-Prot
Match: A5PJN8 (Splicing factor 3A subunit 2 OS=Bos taurus OX=9913 GN=SF3A2 PE=2 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 4.5e-77
Identity = 196/339 (57.82%), Postives = 231/339 (68.14%), Query Frame = 0

Query: 6   GSKPGSGGAATAQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTLHNNEG 65
           G K GSGG A++     DRRERLR+LALETID+ KDPYFM+NHLGSYECKLCLTLHNNEG
Sbjct: 8   GGKTGSGGVASSSESNRDRRERLRQLALETIDINKDPYFMKNHLGSYECKLCLTLHNNEG 67

Query: 66  NYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKTVKIGRPGYRVTKQFDSET 125
           +YLAHTQGK+HQTNLA+RAA+EAKEAPAQP P K KV V+K VKIGRPGY+VTKQ D+E 
Sbjct: 68  SYLAHTQGKKHQTNLARRAAKEAKEAPAQPAPEKVKVEVKKFVKIGRPGYKVTKQRDTEM 127

Query: 126 KQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAAEPYEIIAFKVPSTE 185
            Q+SLLFQI+YPEI +   PRHRFMS+YEQR++P D+R+QYLL AAEPYE IAFKVPS E
Sbjct: 128 GQQSLLFQIDYPEIAEGIMPRHRFMSAYEQRIEPPDRRWQYLLMAAEPYETIAFKVPSRE 187

Query: 186 IDKSTPKFFSHWDPDSKMFTLQLYFKSKPQEANKPPPVPAANGTVPSGAPPRPLPPPPQA 245
           IDK+  KF++HW+ ++K F LQ +FK +     KPP  P    ++P+G P    PPPP  
Sbjct: 188 IDKAEGKFWTHWNRETKQFFLQFHFKME-----KPPAPP----SLPAGPPGVKRPPPPLM 247

Query: 246 PPPPPPPPQQGAPPRVPPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPIGNNTMA 305
              PP PP    P  +PPPP PG LP PP  M    P P  P G P +PPP         
Sbjct: 248 NGLPPRPP---LPESLPPPP-PGGLPLPP--MPPSGPAPSGPPGPPQLPPP--------- 307

Query: 306 NFTPGTQMNRPPMPPPPQGF--PGQGIRQPPP---PPPP 340
              PG     P + PP  G   P  G+  P P   PP P
Sbjct: 308 --APGVHPPAPVVHPPASGVHPPAPGVHPPAPGVHPPAP 320

BLAST of Chy4G087380 vs. ExPASy Swiss-Prot
Match: Q62203 (Splicing factor 3A subunit 2 OS=Mus musculus OX=10090 GN=Sf3a2 PE=1 SV=2)

HSP 1 Score: 270.8 bits (691), Expect = 2.1e-71
Identity = 200/363 (55.10%), Postives = 228/363 (62.81%), Query Frame = 0

Query: 6   GSKPGSGGAATAQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTLHNNEG 65
           G K GSGG A++     DRRERLR+LALETID+ KDPYFM+NHLGSYECKLCLTLHNNEG
Sbjct: 8   GGKTGSGGVASSSESNRDRRERLRQLALETIDINKDPYFMKNHLGSYECKLCLTLHNNEG 67

Query: 66  NYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKTVKIGRPGYRVTKQFDSET 125
           +YLAHTQGK+HQTNLA+RAA+EAKEAPAQP P + KV V+K VKIGRPGY+VTKQ D+E 
Sbjct: 68  SYLAHTQGKKHQTNLARRAAKEAKEAPAQPAPEQVKVEVKKFVKIGRPGYKVTKQRDTEM 127

Query: 126 KQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAAEPYEIIAFKVPSTE 185
            Q+SLLFQI+YPEI +   PRHRFMS+YEQR++P D+R+QYLL AAEPYE IAFKVPS E
Sbjct: 128 GQQSLLFQIDYPEIAEGIMPRHRFMSAYEQRIEPPDRRWQYLLMAAEPYETIAFKVPSRE 187

Query: 186 IDKSTPKFFSHWDPDSKMFTLQLYFKSKPQEAN----------KPPPVPAANGTVPSGAP 245
           IDK+  KF          F LQ +FK +   A           K PP P  NG  P    
Sbjct: 188 IDKAEGKF----------FFLQFHFKMEKPPAPPSLPAGPPGVKRPPPPLMNGLPPRPPL 247

Query: 246 PRPLPPPPQA----PPPPPPPPQQGAPPRVP--PPPMPGSLPPPPSVMANGPP----RPM 305
           P  LPPPP      PP PP  P    PP  P  PPP PG  PP P V    PP     P 
Sbjct: 248 PDALPPPPPGGLPLPPMPPTGPAPSGPPGPPQMPPPAPGVHPPAPVV---HPPTSGVHPP 307

Query: 306 PPGGAPPIP--PPPPIGNNTMANFTPGTQMNRPPMPPPPQGF--PGQGIRQPPP---PPP 342
            PG  PP P   PP  G +  A   PG     P + PP  G   P  G+  P P   PP 
Sbjct: 308 APGVHPPAPVVHPPTSGVHPPA---PGVHPPTPGVHPPAPGVHPPAPGVHPPAPGVHPPT 354

BLAST of Chy4G087380 vs. ExPASy Swiss-Prot
Match: Q54B65 (Splicing factor 3A subunit 2 OS=Dictyostelium discoideum OX=44689 GN=sf3a2 PE=3 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 1.0e-65
Identity = 130/214 (60.75%), Postives = 170/214 (79.44%), Query Frame = 0

Query: 4   EWGSKPGSGGAATAQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTLHNN 63
           E+G K GSGG  ++Q + IDRRER ++L LE +D++KDPY + NH+GS+EC+LCLT+HNN
Sbjct: 3   EYG-KAGSGGLQSSQYDNIDRRERQKQLVLEHVDVSKDPYIISNHIGSFECRLCLTVHNN 62

Query: 64  EGNYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPH---KRKVSVRKTVKIGRPGYRVTKQ 123
            GNYLAHTQGK+HQT+LA+RAA+E +E P+  + +     +V  +KT+KIGRPGY++ KQ
Sbjct: 63  VGNYLAHTQGKKHQTHLARRAAKEQRENPSVSKNNYIQTTRVIHKKTIKIGRPGYKIIKQ 122

Query: 124 FDSETKQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAAEPYEIIAFK 183
            DS+T Q SLLFQI+YPEIE   +PRHR MS++EQRV+  +K YQYLLFAAEPYE IAFK
Sbjct: 123 RDSKTGQLSLLFQIDYPEIESGLQPRHRIMSAFEQRVEQPNKDYQYLLFAAEPYETIAFK 182

Query: 184 VPSTEIDKST---PKFFSHWDPDSKMFTLQLYFK 212
           +P+ EID++T    KFF+HWD  +K FTLQLYFK
Sbjct: 183 IPNKEIDRTTGPDGKFFTHWD-RNKTFTLQLYFK 214

BLAST of Chy4G087380 vs. ExPASy TrEMBL
Match: A0A0A0LEB1 (Matrin-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G743440 PE=3 SV=1)

HSP 1 Score: 644.4 bits (1661), Expect = 2.6e-181
Identity = 338/338 (100.00%), Postives = 338/338 (100.00%), Query Frame = 0

Query: 1   MDREWGSKPGSGGAATAQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTL 60
           MDREWGSKPGSGGAATAQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTL
Sbjct: 1   MDREWGSKPGSGGAATAQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTL 60

Query: 61  HNNEGNYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKTVKIGRPGYRVTKQ 120
           HNNEGNYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKTVKIGRPGYRVTKQ
Sbjct: 61  HNNEGNYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKTVKIGRPGYRVTKQ 120

Query: 121 FDSETKQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAAEPYEIIAFK 180
           FDSETKQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAAEPYEIIAFK
Sbjct: 121 FDSETKQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAAEPYEIIAFK 180

Query: 181 VPSTEIDKSTPKFFSHWDPDSKMFTLQLYFKSKPQEANKPPPVPAANGTVPSGAPPRPLP 240
           VPSTEIDKSTPKFFSHWDPDSKMFTLQLYFKSKPQEANKPPPVPAANGTVPSGAPPRPLP
Sbjct: 181 VPSTEIDKSTPKFFSHWDPDSKMFTLQLYFKSKPQEANKPPPVPAANGTVPSGAPPRPLP 240

Query: 241 PPPQAPPPPPPPPQQGAPPRVPPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPIG 300
           PPPQAPPPPPPPPQQGAPPRVPPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPIG
Sbjct: 241 PPPQAPPPPPPPPQQGAPPRVPPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPIG 300

Query: 301 NNTMANFTPGTQMNRPPMPPPPQGFPGQGIRQPPPPPP 339
           NNTMANFTPGTQMNRPPMPPPPQGFPGQGIRQPPPPPP
Sbjct: 301 NNTMANFTPGTQMNRPPMPPPPQGFPGQGIRQPPPPPP 338

BLAST of Chy4G087380 vs. ExPASy TrEMBL
Match: A0A5D3DPC7 (Splicing factor 3A subunit 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002950 PE=3 SV=1)

HSP 1 Score: 640.2 bits (1650), Expect = 5.0e-180
Identity = 335/338 (99.11%), Postives = 337/338 (99.70%), Query Frame = 0

Query: 1   MDREWGSKPGSGGAATAQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTL 60
           MDREWGSKPGSGGAA+AQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTL
Sbjct: 1   MDREWGSKPGSGGAASAQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTL 60

Query: 61  HNNEGNYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKTVKIGRPGYRVTKQ 120
           HNNEGNYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKTVKIGRPGYRVTKQ
Sbjct: 61  HNNEGNYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKTVKIGRPGYRVTKQ 120

Query: 121 FDSETKQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAAEPYEIIAFK 180
           FDSETKQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAAEPYEIIAFK
Sbjct: 121 FDSETKQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAAEPYEIIAFK 180

Query: 181 VPSTEIDKSTPKFFSHWDPDSKMFTLQLYFKSKPQEANKPPPVPAANGTVPSGAPPRPLP 240
           VPSTEIDKSTPKFFSHWDPDSKMFTLQLYFKSKPQEANKPPPVPAANGTVPSGAPPRPLP
Sbjct: 181 VPSTEIDKSTPKFFSHWDPDSKMFTLQLYFKSKPQEANKPPPVPAANGTVPSGAPPRPLP 240

Query: 241 PPPQAPPPPPPPPQQGAPPRVPPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPIG 300
           PPPQAPPPPPPPPQQGAPPRVPPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPP+G
Sbjct: 241 PPPQAPPPPPPPPQQGAPPRVPPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPVG 300

Query: 301 NNTMANFTPGTQMNRPPMPPPPQGFPGQGIRQPPPPPP 339
           NNTMANFTPGTQMNRPPMPPPPQ FPGQGIRQPPPPPP
Sbjct: 301 NNTMANFTPGTQMNRPPMPPPPQSFPGQGIRQPPPPPP 338

BLAST of Chy4G087380 vs. ExPASy TrEMBL
Match: A0A1S3B729 (splicing factor 3A subunit 2 OS=Cucumis melo OX=3656 GN=LOC103486522 PE=3 SV=1)

HSP 1 Score: 640.2 bits (1650), Expect = 5.0e-180
Identity = 335/338 (99.11%), Postives = 337/338 (99.70%), Query Frame = 0

Query: 1   MDREWGSKPGSGGAATAQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTL 60
           MDREWGSKPGSGGAA+AQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTL
Sbjct: 1   MDREWGSKPGSGGAASAQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTL 60

Query: 61  HNNEGNYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKTVKIGRPGYRVTKQ 120
           HNNEGNYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKTVKIGRPGYRVTKQ
Sbjct: 61  HNNEGNYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKTVKIGRPGYRVTKQ 120

Query: 121 FDSETKQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAAEPYEIIAFK 180
           FDSETKQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAAEPYEIIAFK
Sbjct: 121 FDSETKQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAAEPYEIIAFK 180

Query: 181 VPSTEIDKSTPKFFSHWDPDSKMFTLQLYFKSKPQEANKPPPVPAANGTVPSGAPPRPLP 240
           VPSTEIDKSTPKFFSHWDPDSKMFTLQLYFKSKPQEANKPPPVPAANGTVPSGAPPRPLP
Sbjct: 181 VPSTEIDKSTPKFFSHWDPDSKMFTLQLYFKSKPQEANKPPPVPAANGTVPSGAPPRPLP 240

Query: 241 PPPQAPPPPPPPPQQGAPPRVPPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPIG 300
           PPPQAPPPPPPPPQQGAPPRVPPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPP+G
Sbjct: 241 PPPQAPPPPPPPPQQGAPPRVPPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPVG 300

Query: 301 NNTMANFTPGTQMNRPPMPPPPQGFPGQGIRQPPPPPP 339
           NNTMANFTPGTQMNRPPMPPPPQ FPGQGIRQPPPPPP
Sbjct: 301 NNTMANFTPGTQMNRPPMPPPPQSFPGQGIRQPPPPPP 338

BLAST of Chy4G087380 vs. ExPASy TrEMBL
Match: A0A5A7TQW8 (Splicing factor 3A subunit 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G003160 PE=3 SV=1)

HSP 1 Score: 630.6 bits (1625), Expect = 3.9e-177
Identity = 334/347 (96.25%), Postives = 336/347 (96.83%), Query Frame = 0

Query: 1   MDREWGSKPGSGGAATAQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTL 60
           MDREWGSKPGSGGAA+AQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTL
Sbjct: 1   MDREWGSKPGSGGAASAQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTL 60

Query: 61  HNNEGNYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKT---------VKIG 120
           HNNEGNYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKT          KIG
Sbjct: 61  HNNEGNYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKTGMLLCPSPLFKIG 120

Query: 121 RPGYRVTKQFDSETKQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAA 180
           RPGYRVTKQFDSETKQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAA
Sbjct: 121 RPGYRVTKQFDSETKQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAA 180

Query: 181 EPYEIIAFKVPSTEIDKSTPKFFSHWDPDSKMFTLQLYFKSKPQEANKPPPVPAANGTVP 240
           EPYEIIAFKVPSTEIDKSTPKFFSHWDPDSKMFTLQLYFKSKPQEANKPPPVPAANGTVP
Sbjct: 181 EPYEIIAFKVPSTEIDKSTPKFFSHWDPDSKMFTLQLYFKSKPQEANKPPPVPAANGTVP 240

Query: 241 SGAPPRPLPPPPQAPPPPPPPPQQGAPPRVPPPPMPGSLPPPPSVMANGPPRPMPPGGAP 300
           SGAPPRPLPPPPQAPPPPPPPPQQGAPPRVPPPPMPGSLPPPPSVMANGPPRPMPPGGAP
Sbjct: 241 SGAPPRPLPPPPQAPPPPPPPPQQGAPPRVPPPPMPGSLPPPPSVMANGPPRPMPPGGAP 300

Query: 301 PIPPPPPIGNNTMANFTPGTQMNRPPMPPPPQGFPGQGIRQPPPPPP 339
           PIPPPPP+GNNTMANFTPGTQMNRPPMPPPPQ FPGQGIRQPPPPPP
Sbjct: 301 PIPPPPPVGNNTMANFTPGTQMNRPPMPPPPQSFPGQGIRQPPPPPP 347

BLAST of Chy4G087380 vs. ExPASy TrEMBL
Match: A0A6J1KWM2 (splicing factor 3A subunit 2 OS=Cucurbita maxima OX=3661 GN=LOC111498269 PE=3 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 1.1e-171
Identity = 324/338 (95.86%), Postives = 327/338 (96.75%), Query Frame = 0

Query: 1   MDREWGSKPGSGGAATAQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTL 60
           MDREWGSKPGSGGAA+AQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTL
Sbjct: 1   MDREWGSKPGSGGAASAQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTL 60

Query: 61  HNNEGNYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKTVKIGRPGYRVTKQ 120
           HNNEGNYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKTVKIGRPGYRVTKQ
Sbjct: 61  HNNEGNYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKTVKIGRPGYRVTKQ 120

Query: 121 FDSETKQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAAEPYEIIAFK 180
           FDSETKQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAAEPYEIIAFK
Sbjct: 121 FDSETKQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAAEPYEIIAFK 180

Query: 181 VPSTEIDKSTPKFFSHWDPDSKMFTLQLYFKSKPQEANKPPPVPAANGTVPSGAPPRPLP 240
           VPSTEIDKSTPKFFSHWDPDSKMFTLQLYFKSKPQEANKP  VPAANGTV       PLP
Sbjct: 181 VPSTEIDKSTPKFFSHWDPDSKMFTLQLYFKSKPQEANKPQTVPAANGTV-------PLP 240

Query: 241 PPPQAPPPPPPPPQQGAPPRVPPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPIG 300
           PPPQAPPPPPPPPQQGAPPRVPPPPMPGSLPPPP VMANGPPRPMPPGGAPPIPPPPP+G
Sbjct: 241 PPPQAPPPPPPPPQQGAPPRVPPPPMPGSLPPPPPVMANGPPRPMPPGGAPPIPPPPPVG 300

Query: 301 NNTMANFTPGTQMNRPPMPPPPQGFPGQGIRQPPPPPP 339
           NNTMANFTPGTQMNR PMPPPPQGFPGQG+RQPPPPPP
Sbjct: 301 NNTMANFTPGTQMNRLPMPPPPQGFPGQGVRQPPPPPP 331

BLAST of Chy4G087380 vs. NCBI nr
Match: XP_004137848.1 (splicing factor 3A subunit 2 [Cucumis sativus] >KGN59007.1 hypothetical protein Csa_002498 [Cucumis sativus])

HSP 1 Score: 638 bits (1646), Expect = 4.60e-230
Identity = 340/341 (99.71%), Postives = 340/341 (99.71%), Query Frame = 0

Query: 1   MDREWGSKPGSGGAATAQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTL 60
           MDREWGSKPGSGGAATAQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTL
Sbjct: 1   MDREWGSKPGSGGAATAQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTL 60

Query: 61  HNNEGNYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKTVKIGRPGYRVTKQ 120
           HNNEGNYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKTVKIGRPGYRVTKQ
Sbjct: 61  HNNEGNYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKTVKIGRPGYRVTKQ 120

Query: 121 FDSETKQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAAEPYEIIAFK 180
           FDSETKQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAAEPYEIIAFK
Sbjct: 121 FDSETKQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAAEPYEIIAFK 180

Query: 181 VPSTEIDKSTPKFFSHWDPDSKMFTLQLYFKSKPQEANKPPPVPAANGTVPSGAPPRPLP 240
           VPSTEIDKSTPKFFSHWDPDSKMFTLQLYFKSKPQEANKPPPVPAANGTVPSGAPPRPLP
Sbjct: 181 VPSTEIDKSTPKFFSHWDPDSKMFTLQLYFKSKPQEANKPPPVPAANGTVPSGAPPRPLP 240

Query: 241 PPPQAPPPPPPPPQQGAPPRVPPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPIG 300
           PPPQAPPPPPPPPQQGAPPRVPPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPIG
Sbjct: 241 PPPQAPPPPPPPPQQGAPPRVPPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPIG 300

Query: 301 NNTMANFTPGTQMNRPPMPPPPQGFPGQGIRQPPPPPPPNM 341
           NNTMANFTPGTQMNRPPMPPPPQGFPGQGIRQPPPP PPNM
Sbjct: 301 NNTMANFTPGTQMNRPPMPPPPQGFPGQGIRQPPPP-PPNM 340

BLAST of Chy4G087380 vs. NCBI nr
Match: XP_008442733.1 (PREDICTED: splicing factor 3A subunit 2 [Cucumis melo] >TYK25169.1 splicing factor 3A subunit 2 [Cucumis melo var. makuwa])

HSP 1 Score: 634 bits (1635), Expect = 2.18e-228
Identity = 337/341 (98.83%), Postives = 339/341 (99.41%), Query Frame = 0

Query: 1   MDREWGSKPGSGGAATAQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTL 60
           MDREWGSKPGSGGAA+AQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTL
Sbjct: 1   MDREWGSKPGSGGAASAQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTL 60

Query: 61  HNNEGNYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKTVKIGRPGYRVTKQ 120
           HNNEGNYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKTVKIGRPGYRVTKQ
Sbjct: 61  HNNEGNYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKTVKIGRPGYRVTKQ 120

Query: 121 FDSETKQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAAEPYEIIAFK 180
           FDSETKQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAAEPYEIIAFK
Sbjct: 121 FDSETKQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAAEPYEIIAFK 180

Query: 181 VPSTEIDKSTPKFFSHWDPDSKMFTLQLYFKSKPQEANKPPPVPAANGTVPSGAPPRPLP 240
           VPSTEIDKSTPKFFSHWDPDSKMFTLQLYFKSKPQEANKPPPVPAANGTVPSGAPPRPLP
Sbjct: 181 VPSTEIDKSTPKFFSHWDPDSKMFTLQLYFKSKPQEANKPPPVPAANGTVPSGAPPRPLP 240

Query: 241 PPPQAPPPPPPPPQQGAPPRVPPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPIG 300
           PPPQAPPPPPPPPQQGAPPRVPPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPP+G
Sbjct: 241 PPPQAPPPPPPPPQQGAPPRVPPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPVG 300

Query: 301 NNTMANFTPGTQMNRPPMPPPPQGFPGQGIRQPPPPPPPNM 341
           NNTMANFTPGTQMNRPPMPPPPQ FPGQGIRQPPPP PPNM
Sbjct: 301 NNTMANFTPGTQMNRPPMPPPPQSFPGQGIRQPPPP-PPNM 340

BLAST of Chy4G087380 vs. NCBI nr
Match: XP_038905938.1 (splicing factor 3A subunit 2 [Benincasa hispida])

HSP 1 Score: 630 bits (1626), Expect = 5.14e-227
Identity = 335/341 (98.24%), Postives = 338/341 (99.12%), Query Frame = 0

Query: 1   MDREWGSKPGSGGAATAQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTL 60
           MDREWGSKPGSGGAA+AQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTL
Sbjct: 1   MDREWGSKPGSGGAASAQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTL 60

Query: 61  HNNEGNYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKTVKIGRPGYRVTKQ 120
           HNNEGNYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKTVKIGRPGYRVTKQ
Sbjct: 61  HNNEGNYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKTVKIGRPGYRVTKQ 120

Query: 121 FDSETKQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAAEPYEIIAFK 180
           FDSETKQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAAEPYEIIAFK
Sbjct: 121 FDSETKQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAAEPYEIIAFK 180

Query: 181 VPSTEIDKSTPKFFSHWDPDSKMFTLQLYFKSKPQEANKPPPVPAANGTVPSGAPPRPLP 240
           VPSTEIDKSTPKFFSHWDPDSKMFTLQLYFKSKPQEANKPPPVPAANGTVPSGAPPRP+P
Sbjct: 181 VPSTEIDKSTPKFFSHWDPDSKMFTLQLYFKSKPQEANKPPPVPAANGTVPSGAPPRPMP 240

Query: 241 PPPQAPPPPPPPPQQGAPPRVPPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPIG 300
           PPPQAP PPPPPPQQG PPRVPPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPP+G
Sbjct: 241 PPPQAPAPPPPPPQQGPPPRVPPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPVG 300

Query: 301 NNTMANFTPGTQMNRPPMPPPPQGFPGQGIRQPPPPPPPNM 341
           NNTMANFTPGTQMNRPPMPPPPQGFPGQGIRQPPPP PPNM
Sbjct: 301 NNTMANFTPGTQMNRPPMPPPPQGFPGQGIRQPPPP-PPNM 340

BLAST of Chy4G087380 vs. NCBI nr
Match: KAA0043971.1 (splicing factor 3A subunit 2 [Cucumis melo var. makuwa])

HSP 1 Score: 624 bits (1610), Expect = 1.97e-224
Identity = 336/350 (96.00%), Postives = 338/350 (96.57%), Query Frame = 0

Query: 1   MDREWGSKPGSGGAATAQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTL 60
           MDREWGSKPGSGGAA+AQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTL
Sbjct: 1   MDREWGSKPGSGGAASAQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTL 60

Query: 61  HNNEGNYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKT---------VKIG 120
           HNNEGNYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKT          KIG
Sbjct: 61  HNNEGNYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKTGMLLCPSPLFKIG 120

Query: 121 RPGYRVTKQFDSETKQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAA 180
           RPGYRVTKQFDSETKQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAA
Sbjct: 121 RPGYRVTKQFDSETKQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAA 180

Query: 181 EPYEIIAFKVPSTEIDKSTPKFFSHWDPDSKMFTLQLYFKSKPQEANKPPPVPAANGTVP 240
           EPYEIIAFKVPSTEIDKSTPKFFSHWDPDSKMFTLQLYFKSKPQEANKPPPVPAANGTVP
Sbjct: 181 EPYEIIAFKVPSTEIDKSTPKFFSHWDPDSKMFTLQLYFKSKPQEANKPPPVPAANGTVP 240

Query: 241 SGAPPRPLPPPPQAPPPPPPPPQQGAPPRVPPPPMPGSLPPPPSVMANGPPRPMPPGGAP 300
           SGAPPRPLPPPPQAPPPPPPPPQQGAPPRVPPPPMPGSLPPPPSVMANGPPRPMPPGGAP
Sbjct: 241 SGAPPRPLPPPPQAPPPPPPPPQQGAPPRVPPPPMPGSLPPPPSVMANGPPRPMPPGGAP 300

Query: 301 PIPPPPPIGNNTMANFTPGTQMNRPPMPPPPQGFPGQGIRQPPPPPPPNM 341
           PIPPPPP+GNNTMANFTPGTQMNRPPMPPPPQ FPGQGIRQPPPP PPNM
Sbjct: 301 PIPPPPPVGNNTMANFTPGTQMNRPPMPPPPQSFPGQGIRQPPPP-PPNM 349

BLAST of Chy4G087380 vs. NCBI nr
Match: XP_023005145.1 (splicing factor 3A subunit 2 [Cucurbita maxima])

HSP 1 Score: 606 bits (1563), Expect = 1.58e-217
Identity = 326/341 (95.60%), Postives = 329/341 (96.48%), Query Frame = 0

Query: 1   MDREWGSKPGSGGAATAQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTL 60
           MDREWGSKPGSGGAA+AQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTL
Sbjct: 1   MDREWGSKPGSGGAASAQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTL 60

Query: 61  HNNEGNYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKTVKIGRPGYRVTKQ 120
           HNNEGNYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKTVKIGRPGYRVTKQ
Sbjct: 61  HNNEGNYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKTVKIGRPGYRVTKQ 120

Query: 121 FDSETKQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAAEPYEIIAFK 180
           FDSETKQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAAEPYEIIAFK
Sbjct: 121 FDSETKQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAAEPYEIIAFK 180

Query: 181 VPSTEIDKSTPKFFSHWDPDSKMFTLQLYFKSKPQEANKPPPVPAANGTVPSGAPPRPLP 240
           VPSTEIDKSTPKFFSHWDPDSKMFTLQLYFKSKPQEANKP  VPAANGTVP       LP
Sbjct: 181 VPSTEIDKSTPKFFSHWDPDSKMFTLQLYFKSKPQEANKPQTVPAANGTVP-------LP 240

Query: 241 PPPQAPPPPPPPPQQGAPPRVPPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPIG 300
           PPPQAPPPPPPPPQQGAPPRVPPPPMPGSLPPPP VMANGPPRPMPPGGAPPIPPPPP+G
Sbjct: 241 PPPQAPPPPPPPPQQGAPPRVPPPPMPGSLPPPPPVMANGPPRPMPPGGAPPIPPPPPVG 300

Query: 301 NNTMANFTPGTQMNRPPMPPPPQGFPGQGIRQPPPPPPPNM 341
           NNTMANFTPGTQMNR PMPPPPQGFPGQG+RQPPPP PPNM
Sbjct: 301 NNTMANFTPGTQMNRLPMPPPPQGFPGQGVRQPPPP-PPNM 333

BLAST of Chy4G087380 vs. TAIR 10
Match: AT2G32600.1 (hydroxyproline-rich glycoprotein family protein )

HSP 1 Score: 416.0 bits (1068), Expect = 2.9e-116
Identity = 227/289 (78.55%), Postives = 237/289 (82.01%), Query Frame = 0

Query: 1   MDREWGSKPGSGGAATAQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTL 60
           MDREWGSKPGSGGAA+ QNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTL
Sbjct: 1   MDREWGSKPGSGGAASGQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTL 60

Query: 61  HNNEGNYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKTVKIGRPGYRVTKQ 120
           HNNEGNYLAHTQGKRHQTNLAKRAAREAK+AP +PQP KR VSVR+TVKIGRPGYRVTKQ
Sbjct: 61  HNNEGNYLAHTQGKRHQTNLAKRAAREAKDAPTKPQPLKRNVSVRRTVKIGRPGYRVTKQ 120

Query: 121 FDSETKQRSLLFQIEYPEIEDLAKPRHRFMSSYEQRVQPFDKRYQYLLFAAEPYEIIAFK 180
           +D E +QRSLLFQIEYPEIED  KPRHRFMSSYEQ+VQP+DK YQYLLFAAEPYEIIAFK
Sbjct: 121 YDPELQQRSLLFQIEYPEIEDNIKPRHRFMSSYEQKVQPYDKSYQYLLFAAEPYEIIAFK 180

Query: 181 VPSTEIDKSTPKFFSHWDPDSKMFTLQLYFKSKPQEANKPPPVPAANGTVPSGAPPRPLP 240
           VPSTE+DKSTPKFFSHWDPDSKMFTLQ+YFK    E NKP     ANG            
Sbjct: 181 VPSTEVDKSTPKFFSHWDPDSKMFTLQVYFKPTKPEPNKPQSAVGANG------------ 240

Query: 241 PPPQAPPPPPPPPQQGAPPRVPPPPMPGSLPPPPSVMANGPPRPMPPGG 290
                PPPPPPPP Q  P   PPPP  G  PPPP  MAN   RPMPP G
Sbjct: 241 ----LPPPPPPPPHQAQP---PPPPPSGLFPPPPPPMANNGFRPMPPAG 270

BLAST of Chy4G087380 vs. TAIR 10
Match: AT5G07740.1 (actin binding )

HSP 1 Score: 47.0 bits (110), Expect = 3.6e-05
Identity = 64/126 (50.79%), Postives = 68/126 (53.97%), Query Frame = 0

Query: 220  PPPVPAANGTVPSGAPPRPLPPPPQ--APPPPPPPPQQ-GAPPRVPPPPMPGSLPPPPSV 279
            PPP P  +G    GAPP P PPP    APPPPPPPP   GAPP  PPPPM G  PPPP  
Sbjct: 1046 PPPPPPMHG----GAPPPPPPPPMHGGAPPPPPPPPMHGGAPPPPPPPPMHGGAPPPPPP 1105

Query: 280  MANGPPRPMPP----GGAPPIPPPPPIGNNTMANFTPGTQMNRPPMPPPPQGFPGQGIRQ 339
               G  +P PP    GGAPP PPPPP+         P  +   PP PPPP      G   
Sbjct: 1106 PMFGGAQPPPPPPMRGGAPP-PPPPPMRGGAPPPPPPPMRGGAPPPPPPPM----HGGAP 1162

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q154281.4e-7856.51Splicing factor 3A subunit 2 OS=Homo sapiens OX=9606 GN=SF3A2 PE=1 SV=2[more]
Q6AXT86.9e-7857.14Splicing factor 3A subunit 2 OS=Rattus norvegicus OX=10116 GN=Sf3a2 PE=2 SV=1[more]
A5PJN84.5e-7757.82Splicing factor 3A subunit 2 OS=Bos taurus OX=9913 GN=SF3A2 PE=2 SV=1[more]
Q622032.1e-7155.10Splicing factor 3A subunit 2 OS=Mus musculus OX=10090 GN=Sf3a2 PE=1 SV=2[more]
Q54B651.0e-6560.75Splicing factor 3A subunit 2 OS=Dictyostelium discoideum OX=44689 GN=sf3a2 PE=3 ... [more]
Match NameE-valueIdentityDescription
A0A0A0LEB12.6e-181100.00Matrin-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G743440... [more]
A0A5D3DPC75.0e-18099.11Splicing factor 3A subunit 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3B7295.0e-18099.11splicing factor 3A subunit 2 OS=Cucumis melo OX=3656 GN=LOC103486522 PE=3 SV=1[more]
A0A5A7TQW83.9e-17796.25Splicing factor 3A subunit 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca... [more]
A0A6J1KWM21.1e-17195.86splicing factor 3A subunit 2 OS=Cucurbita maxima OX=3661 GN=LOC111498269 PE=3 SV... [more]
Match NameE-valueIdentityDescription
XP_004137848.14.60e-23099.71splicing factor 3A subunit 2 [Cucumis sativus] >KGN59007.1 hypothetical protein ... [more]
XP_008442733.12.18e-22898.83PREDICTED: splicing factor 3A subunit 2 [Cucumis melo] >TYK25169.1 splicing fact... [more]
XP_038905938.15.14e-22798.24splicing factor 3A subunit 2 [Benincasa hispida][more]
KAA0043971.11.97e-22496.00splicing factor 3A subunit 2 [Cucumis melo var. makuwa][more]
XP_023005145.11.58e-21795.60splicing factor 3A subunit 2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT2G32600.12.9e-11678.55hydroxyproline-rich glycoprotein family protein [more]
AT5G07740.13.6e-0550.79actin binding [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR01217PRICHEXTENSNcoord: 236..252
score: 50.59
coord: 255..272
score: 33.33
coord: 215..236
score: 30.91
coord: 277..302
score: 27.69
NoneNo IPR availableSMARTSM01050CactinC_cactus_3coord: 89..216
e-value: 6.7E-19
score: 78.8
NoneNo IPR availablePFAMPF12874zf-metcoord: 52..76
e-value: 1.9E-6
score: 28.0
NoneNo IPR availableGENE3D2.60.40.2690coord: 93..219
e-value: 1.7E-38
score: 133.4
NoneNo IPR availableGENE3D3.30.160.60Classic Zinc Fingercoord: 46..89
e-value: 3.4E-5
score: 25.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 313..342
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 217..302
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 214..342
NoneNo IPR availablePANTHERPTHR23205SPLICING FACTOR 3A SUBUNIT 2coord: 1..335
IPR003604Matrin/U1-C-like, C2H2-type zinc fingerSMARTSM00451ZnF_U1_5coord: 49..83
e-value: 9.0E-8
score: 41.8
IPR031781SF3A2 domainPFAMPF16835SF3A2coord: 110..204
e-value: 7.7E-35
score: 119.1
IPR000690Matrin/U1-C, C2H2-type zinc fingerPROSITEPS50171ZF_MATRINcoord: 52..82
score: 9.295713
IPR036236Zinc finger C2H2 superfamilySUPERFAMILY57667beta-beta-alpha zinc fingerscoord: 48..83

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy4G087380.1Chy4G087380.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000245 spliceosomal complex assembly
cellular_component GO:0071013 catalytic step 2 spliceosome
cellular_component GO:0005686 U2 snRNP
cellular_component GO:0071004 U2-type prespliceosome
cellular_component GO:0005634 nucleus
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008270 zinc ion binding